CN102676383B - Device for quickly detecting pathogen of infectious diseases on site and method thereof - Google Patents
Device for quickly detecting pathogen of infectious diseases on site and method thereof Download PDFInfo
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- CN102676383B CN102676383B CN201210191160XA CN201210191160A CN102676383B CN 102676383 B CN102676383 B CN 102676383B CN 201210191160X A CN201210191160X A CN 201210191160XA CN 201210191160 A CN201210191160 A CN 201210191160A CN 102676383 B CN102676383 B CN 102676383B
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Abstract
The invention discloses a device for quickly detecting pathogen of infectious diseases on site and a method thereof, and relates to a medical detection device. The device consists of a nucleic acid extracting bin (1), a funnel-shaped conduit (2), a nucleic acid amplification reaction bin (3), a cylindrical conduit (4) and a product development bin (5) which are connected from top to bottom in sequence. The method comprises the following steps: 1, adding a sample solution of pathogen of infectious diseases into the nucleic acid extracting bin (1), and extracting nucleic acid by using a sediment boiling method; 2, allowing the nucleic acid to enter the nucleic acid amplification reaction bin (3) through the funnel-shaped conduit (2), and amplifying the nucleic acid through constant temperature water bath to obtain a nucleic acid product; and 3, allowing the nucleic acid product to enter the product development bin (5) through the cylindrical conduit (4), performing nucleic acid dye reaction, developing, and judging a final result according to the visual color change. The device can quickly detects the pathogen of infectious diseases on site, is simple, portable, easy to operate and low in cost, and does not require special instruments or reagents.
Description
Technical field
The present invention relates to a kind of medical science proofing unit, relate in particular to a kind of devices and methods therefor of field quick detection infectious disease pathogens.
Background technology
The invention of polymerase chain reaction (Polymerase Chain Reaction, PCR) has epoch-making meaning, makes the people can be at the specific nucleic acid fragment of external on purpose unlimited amplification.Its principle is similar in the body of DNA and copies, and external synthetic a kind of conditions suitable that provides of DNA just is provided in centrifuge tube.Through development for many years, this technology is very ripe, has derived simultaneously other a lot of correlation technique:, as fluorescent quantitative PCR technique etc., has been most important and the most frequently used technology in molecular biology research and clinical diagnosis field now; Wherein the appearance of PCR instrument makes this technology realize automatization, make the technology application of PCR more extensive, for example in the diagnosing hereditary illness, detect clinical samples in pathogenic agent nucleic acid, legal medical expert's sample made genetics identify, and the aspects such as sudden change situation of analyzing in activated oncogene all are widely used.
Because existing whole proliferation time is long, also more complicated of amplification procedure, result is not easy repetition, instrument costliness and be inconvenient to carry, can not diagnose timely and effectively the related diseases substance as certain outbreaks of infectious diseases, delayed the time, during epidemic situation may, with uncontrollable, cause situation out of control, cause unnecessary loss, so the instrument and equipment that existing nucleic acid amplification technologies uses are restricted in the context of detection of new sudden transmissible disease.
So developing a kind of simple and easy, portable, easy-operating proofing unit is very urgent and necessary new burst infectious disease pathogens are detected, control and try to gain time precious to one for the treatment of new sudden transmissible disease and epidemic situation, this will be following main developing direction.
Summary of the invention:
Purpose of the present invention just is to overcome the shortcoming and defect that prior art exists, and a kind of devices and methods therefor of field quick detection infectious disease pathogens simple and easy, portable, simple to operate is provided.
For achieving the above object, the present invention is by the following technical solutions:
One, the device of field quick detection infectious disease pathogens (abbreviation device)
This device is comprised of the nucleic acid extraction storehouse 1 that connects successively up and down, funnel-form conduit 2, nucleic acid amplification reaction storehouse 3, cylindric conduit 4 and product colour developing storehouse 5.
Two, the method for field quick detection infectious disease pathogens (abbreviation method)
Present method comprises the following steps:
1. add the infectious disease pathogens sample solution in nucleic acid extraction storehouse 1, utilize the precipitation boiling method to extract nucleic acid;
2. nucleic acid is entered nucleic acid amplification reaction storehouse 3 through funnel-form conduit 2,, through water bath with thermostatic control, make nucleic acid amplification, obtain nucleic acid product;
3. nucleic acid product is entered product colour developing storehouse 5 through cylindric conduit 4, colour developing after reacting by nucleic acid dye, according to the colour-change judgement net result of visual colour developing.
The present invention has following advantages and positively effect:
1. can realize the field quick detection infectious disease pathogens;
2. install simple and easy, portable, easy to operate;
3. with low cost, need not specific apparatus and reagent.
Description of drawings:
Fig. 1 is the structural representation of this device;
In figure:
1-nucleic acid extraction storehouse;
2-funnel-form conduit;
3-nucleic acid amplification reaction storehouse;
The cylindric conduit of 4-;
5-product colour developing storehouse.
Embodiment
One, device
1, overall
As Fig. 1, this device is comprised of the nucleic acid extraction storehouse 1 that connects successively up and down, funnel-form conduit 2, nucleic acid amplification reaction storehouse 3, cylindric conduit 4 and product colour developing storehouse 5.
2, functional component
1) the nucleic acid extraction storehouse 1
Nucleic acid extraction storehouse 1 is a kind of container that is provided with upper and lower opening.
2) the funnel-form conduit 2
Funnel-form conduit 2 is a kind of general parts.
3) the nucleic acid amplification reaction storehouse 3
Nucleic acid amplification reaction storehouse 3 is a kind of containers that are provided with upper and lower opening.
4) cylindric conduit 4
5) product colour developing storehouse 5
Product colour developing storehouse 5 is a kind of containers that are provided with upper shed.
3, using method
Utilize this device rapid detection infectious disease pathogens:
During detection, the 40 microlitre infectious disease pathogens samples of getting are joined in nucleic acid extraction storehouse 1, then add 10 microlitre lysis buffers, precipitation is boiled after 10 minutes and is extracted nucleic acid;
Nucleic acid is imported to nucleic acid amplification reaction storehouse 3 by funnel-form conduit 2, be placed in water-bath (being the adjustable equipment of a kind of temperature, is liquid in it) and react with the previously prepd reaction solution, 65 ℃, 60min, obtain nucleic acid product;
Nucleic acid product is imported product colour developing storehouse 5 by cylindric conduit 4, by dyestuff, developed the color detection;
Colour-change judgement net result according to visual colour developing.
Claims (1)
1. the device of field quick detection infectious disease pathogens is characterized in that:
Formed by the nucleic acid extraction storehouse (1) that connects successively up and down, funnel-form conduit (2), nucleic acid amplification reaction storehouse (3), cylindric conduit (4) and product colour developing storehouse (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210191160XA CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210191160XA CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102676383A CN102676383A (en) | 2012-09-19 |
| CN102676383B true CN102676383B (en) | 2013-11-20 |
Family
ID=46808939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210191160XA Expired - Fee Related CN102676383B (en) | 2012-06-11 | 2012-06-11 | Device for quickly detecting pathogen of infectious diseases on site and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102676383B (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7608399B2 (en) * | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| WO2009002511A1 (en) * | 2007-06-26 | 2008-12-31 | Applera Corporation | Nucleic acid sample preparation by exclusion of dna |
| CN202658156U (en) * | 2012-06-11 | 2013-01-09 | 武汉大学 | Device for quickly detecting infectious pathogen on site |
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2012
- 2012-06-11 CN CN201210191160XA patent/CN102676383B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102676383A (en) | 2012-09-19 |
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| C06 | Publication | ||
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| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Yingle Inventor after: Shi Kang Inventor after: Xiang Rong Inventor before: Xiang Rong |
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| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: XIANG RONG TO: LIU YINGLE SHI KANG XIANG RONG |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131120 Termination date: 20140611 |
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| EXPY | Termination of patent right or utility model |