CN102674543A - Magnetoelectric induction bioreactor for rejuvenating bacteria - Google Patents

Magnetoelectric induction bioreactor for rejuvenating bacteria Download PDF

Info

Publication number
CN102674543A
CN102674543A CN2012101657740A CN201210165774A CN102674543A CN 102674543 A CN102674543 A CN 102674543A CN 2012101657740 A CN2012101657740 A CN 2012101657740A CN 201210165774 A CN201210165774 A CN 201210165774A CN 102674543 A CN102674543 A CN 102674543A
Authority
CN
China
Prior art keywords
magnetic field
phase separation
electric field
reactor
separation area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012101657740A
Other languages
Chinese (zh)
Inventor
李天煜
吴宇
韦峰
杨孔萍
刘成良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guilin University of Electronic Technology
Original Assignee
Guilin University of Electronic Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guilin University of Electronic Technology filed Critical Guilin University of Electronic Technology
Priority to CN2012101657740A priority Critical patent/CN102674543A/en
Publication of CN102674543A publication Critical patent/CN102674543A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06Magnetic means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18Flow directing inserts
    • C12M27/20Baffles; Ribs; Ribbons; Auger vanes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Clinical Laboratory Science (AREA)
  • Water Treatment By Electricity Or Magnetism (AREA)

Abstract

The invention discloses a magnetoelectric induction bioreactor for rejuvenating bacteria. The magnetoelectric induction bioreactor for rejuvenating the bacteria comprises a reactor body, a water inlet buffer region, an up-flow reactor, a three-phase separation region and a peripheral water bath heat-preserving sleeve, wherein the water inlet buffer region, the up-flow reactor and the three-phase separation region are arranged in the reactor from bottom to top. The magnetoelectric induction bioreactor for rejuvenating the bacteria is characterized in that the three-phase separation region comprises a primary three-phase separation region and a secondary three-phase separation region; a magnetic field generation system and an electric field generation system are arranged in the reactor body; a circulating mixing system is arranged at the bottom of the pool; and the magnetic field generation system and the electric field generation system are connected with a control system. The reactor can be used for activating the bacteria with reduced bioactivity or close to inactivation, reversing the inactivation process and recovering the bioactivity of bacterial cells so as to achieve the aim of rejuvenation.

Description

A kind of magnetoelectricity that is used for the rejuvenation bacterium is induced bio-reactor
Technical field
The invention belongs to the bio-reactor field, relate in particular to a kind of bio-reactor that adopts magnetoelectricity inductive method rejuvenation bacterium.
Background technology
A lot of bacteriums; Particularly those mankind need utilize its certain specific character behaviour class that the bacterium of certain service is provided; Can appear at efficient operation for some time; The situation that certain given activity descends even loses; Need introduce the service function that new bacteria could keep this performance efficiently repeatedly, for example people will run into such situation when research Anammox denitrifying bacteria biological denitrificaion is active: the denitrification activity of anaerobic ammonium oxidizing bacteria can be with the lengthening of bacterium denitrogenation time inactivation so that completely lose even blackening, fouling gradually, therefore timely rejuvenation bacterium; The stability that keeps its denitrification activity is the prerequisite of carrying out practical applications, eliminating polluted by nitrogen, assurance nitrogen cycle for the mankind.
Anammox is the important nitrogen cycle stage of nature; It also is the frontier of wastewater biological denitrificaion research; It has the effect that can not be substituted in the engineering utilization of eliminating the water body polluted by nitrogen; Having extensively and profound application prospect, is one of maximum research focus in denitride technology basin in the world at present.For utilizing the Anammox effect to carry out the research of engineering practice, though very popular in the world, in China; These research many places are in laboratory stage; One of them subject matter is after operation for some time, the phenomenon that denitrification activity is lost gradually often to take place, if the untimely certain measure of taking at anaerobic ammonia oxidizing bacteria; Mass mortality can take place and disintegrate the consequence that the thalline blackout fouls in the bacterium of last inactivation.
Electrotransformation has easy and simple to handle, quick, transformation efficiency advantages of higher as a kind of foreign DNA transfer method of routine.The method that transforms through electricity realizes the transfer of genetic material, makes the bacterium that loses certain specific character regain this specific character, also can accelerate the heritable variation of bacterium, is the rejuvenation bacterium, obtains the domestic method that certain has the specified property bacterium.Electrotransformation is existing many successful application in the actinomycetes genetic manipulation, as muta lead mycillin ( Streptomyces lividans), streptomyces parvus ( S. parvulus), the wine red streptomyces ( S. vinaceus), streptomyces rimosus ( S. rimosus) wait the report that success is all arranged.
The mangneto biological effect is the magnetic effect hot research fields always, and big quantity research shows both at home and abroad, and the action of a magnetic field can cause the series of biological reaction in vivo behind organism.Part expert thinks that magnetic field can influence biological intravital protein macromolecule and enzyme, makes organism endoenzyme increased activity, improves enzyme reaction rate; In addition, the permeability of biological cell membrane also can be improved in magnetic field, makes water and oxygen molecule and some can receive magnetic action activatory metals ion etc. to be easy to get into cell.Through the biological effect experiment of magnetization in sewage treatment, in the time of can finding that magneticflux-density is 0.37T, aerobic microbiological is active to increase by 17.3%, and the primary productivity of algae is by 8.4-10g/m every day of control samples 3Bring up to 16.9g/m every day 3The desaturase Decolorant Test shows that the existence in magnetic field is favourable to microbial growth and metabolism, thereby promotes organic absorption and utilization are strengthened the ability of microbiological oxidation degradation of organic substances.Therefore, mangneto biological effect and electricity (field) cause biological effect and in the rejuvenation bacterium, have vital role.
High-voltage electric field that use adds and magnetic field; Based on the biological effect of biological dielectric substance to the stress response research magnetoelectricity effect of compound magnetoelectric field; In experiment, confirm and obtain " frequency effect window ", " power (amplitude) the effect window " and " window action time " of certain bacterial strain, find the biological effect rule, activation or rejuvenation are losing certain active bacterial strain gradually; Make bacterial strain recover active again; Keeping its certain active state of being beneficial to man, is human productive life service to its specific trait of human use, is the effective way that often runs into and put the axe in the helve during mikrobe utilizes.
At present; Domestic " device for electro-biologic treatment of the organic wastewater with difficult degradation thereby " patent (publication number: CN1435379) that is useful on; The patent that " is used to handle the magnetic bioreaction separation device of organic waste water " (publication number: CN201593005U) etc., but still useless in bacterium being carried out magnetoelectricity inductive bio-reactor.And research shows, no matter is magnetic field treatment and electric field treatment, as long as intensity is suitable, can produce useful effect to biology, particularly to the rejuvenation process of some degenerative strain, has promoter action preferably.
Summary of the invention:
Technical problem to be solved by this invention provides a kind of magnetoelectricity induction type bio-reactor that can produce the specific magnetic fields electric field; This reactor drum can play the stimulation inducing action to the inactivation bacterial cell; Cause that the bacterium that is in inactivation is in certain change of physiological and biochemical procedure generation; Thereby the reverse inactivation makes the bacterial cell activity of positive inactivation be able to recover, and reaches the purpose of rejuvenation.
The technical scheme that realizes the object of the invention is following:
A kind of magnetoelectricity that is used for the rejuvenation bacterium is induced bio-reactor; Comprise that reactor body and reactor body water inlet buffer zone, flow lifting type reaction chamber, three-phase separation area, magnetic field and electric field from bottom to top produces the water bath heat preservation cover of system and periphery; Different with prior art is: three-phase separation area comprises one-level three-phase separation area and secondary three-phase separation area; In reactor body, also be arranged on magnetic field and produce system and electric field generation system; Also be provided with the internal recycle mixing and stirring system at the bottom of the pond, magnetic field produces system and links to each other with system with electric field generation system.
The tripping device of the one-level three-phase separation area of described three-phase separation area is an active type, and its underpart is nested in flow lifting type reaction zone inwall, and two ears are extended on top, and back-off can separate with reactor body when needing in the inwall of secondary three-phase separation area lower end; Totally 6 on secondary three-phase separation area set inside baffle plate, baffle plate is an active type, is fixed on the secondary triphase separator with draw-in groove, and the number of internal baffle can reduce or increase in case of necessity, and subregion communicates with thrust-augmenting nozzle between baffle plate.
Described electric field generation system comprise be contained in the reaction zone bottom relatively to energising carbon-point and pole plate, feed the stable DC electric current during use, produce stable radial pattern electric field.Through changing size of current, can produce the electric field of varying strength; Through changing pole plate and carbon-point direction, can produce the electric field of different directions, electric field generation system links to each other with system, external system can freely be controlled the power of strength of electric field, and the time of electric field action, in proper order, mode.
Described magnetic field generation system comprise be loaded on the reaction zone bottom another relatively to or the energising solenoid of upward and downward, feed the stable DC electric current during use, coil inside produces stable uniform magnetic field.Through changing size of current, the magnetic field that can produce varying strength; Through changing energising solenoid direction, the magnetic field that can produce different directions, magnetic field produce system and link to each other with system, the power that external system can free controlling magnetic field intensity, and the time of the action of a magnetic field, in proper order, mode.
The internal recycle whipping appts is that characteristic is at the bottom of the described pond; Obtain of the suction of internal-suction type internal recycle whipping appts through being submerged in the bottom to mud mixture; Comprise location suction and portable suction, can make to be deposited in the into mud and the continuous tumble mixed of top mud of water buffer zone bottom, guarantee that sedimentary mud can both fully get into magnetoelectricity and induce the district; Accept the magnetoelectricity inducing action, make the top layer heavy-oxygen-enriched water be difficult for sneaking into lower floor again.
Advantage of the present invention and beneficial effect
1) technology is new: the magnetoelectricity inductive technology combines to realize cultivation, the rejuvenation effect to bacterium, reaches the purpose that activates the inactivation anaerobic ammonia oxidizing bacteria.Single electric inductive technology or magnetic inductive technology are difficult to activate inactivation or the just anaerobic ammonia oxidizing bacteria of inactivation.This reactor drum is through providing a kind of magnetoelectricity induction type bio-reactor that can produce specific electric field magnetic field; Combination activation technique through electric field, magnetic field; Play stimulation inducing action, cause the bacterium that is in inactivation in certain change of physiological and biochemical procedure generation, thereby reverse inactivation the inactivation bacterial cell; Thereby the bacterial cell activity that makes positive inactivation is able to recover, and reaches the purpose of rejuvenation.
2) abundant, the uniform mixing of mud of magnetoelectricity inducing action is accepted in assurance: through inhaling the suction of recirculation unit in the sinking of internal recycle whipping appts at the bottom of the pond; Make and be deposited in the into mud and the continuous tumble mixed of top mud of water buffer zone bottom; Guarantee that sedimentary mud can both fully get into magnetoelectricity and induce the district; Accept the magnetoelectricity inducing action, and reduce sneaking into of oxygen as far as possible.
3) be beneficial to research each item operational factors, indicator reaction: through the regulation and control combination of reaction conditions such as electric field, magnetic field, temperature control or parameter; Help exploring the parameters of mobilizing function inactivation bacterium, like the righttest voltage reach, the galvanic action scope; The frequency effect scope; To obtain " the righttest effect window " of each parameter,, guarantee activation efficiency to greatest extent for reactor drum provides condition in the operation of the best use of window; And make under the same terms such as temperature, influent quality, the comparative study magnetoelectricity induces the difference on bacterial classification and the non-inducing culture bacterial classification Physiology and biochemistry activity to become possibility.
Description of drawings
Fig. 1 is the structural representation of reactor drum among the embodiment.
Embodiment
Below in conjunction with accompanying drawing the present invention is described further:
See Fig. 1, reactor lower part water inlet buffer zone 1 of the present invention is a quadrilateral pyramid, and the bottom is provided with water inlet pipe 10; Buffer zone 1 is a flow lifting type reaction zone 2 up, is square, and up be three-phase separation area again; Three phase separation is divided into one-level three-phase separation area 3 and secondary three-phase separation area 4, and three-phase separation area top is provided with rising pipe 11, and the top is equipped with sealing cover and is provided with air outlet 6; The magnetoelectricity apparatus for deivation is equipped with in flow lifting type reaction zone 2 bottoms; Comprise the major portion energising carbon-point 7-1 and the pole plate 7-2 that produce electric field, produce the major portion energising solenoid 8 in magnetic field, internal recycle whipping appts 9.Anaerobic ammonia oxidizing bacteria is present in the flow lifting type reaction zone 2 with the form of granule sludge.The reactor body outside is provided with water bath heat preservation cover 5, and its length and width are 1.5-2 times of flow lifting type reaction zone 2, and are highly equal, can be used for keeping the reactor cell temperature.
Described pyramid water inlet buffer zone 1 length and width height ratio is 1:1:1.5, and the lower bevel inclination angle is 65o-75o, and the flow lifting type reaction zone connects into water buffer zone 1 upper end for 2 times, on connect the three-phase separation area lower end, it highly accounts for the 2/3-3/5 of reactor body height.One-level three-phase separating device 3 is built between reaction zone 2 and the secondary three-phase separation area 4, is both ends open, the middle two ends symmetric form rib body that shrinks, and its overall length and width height ratio is 1:1: (2-2.5).Secondary three-phase separation area 4 directly is connected on the flow lifting type reaction zone 2, highly is the 1/3-1/2 of reaction zone 2 height, and the upper end is provided with the active type sealing cover, and sealing cover is provided with air outlet 6.
Described three phase separation zone device is divided into active type one-level three-phase separating device 3 and fixed three-phase separation area 4 two portions; Active type 3 its underparts are nested in flow lifting type reaction zone 2 inwalls; Two ear 3-1 are extended on top; Back-off can separate with reactor body when needing in the inwall of secondary three-phase separation area lower end.Constriction 3-2 sectional area is the 1/3-2/5 of port area.Fixed triphase separator 4 is (0.3-0.4) with the TV ratio of said reactor body: 1; The angle β of its flaring wall and datum water level is 45o-70o, totally 6 of set inside baffle plate 4-2, and baffle plate is all fixed with draw-in groove; Detachable in case of necessity; The angle γ of itself and datum water level is 65o-85o, and outer side shield and interior side shield vertex and rising pipe height maintain an equal level, and middle baffle plate height and reactor head are fair.The baffle plate bottom is 3-7cm with flaring wall inwall spacing.Subregion communicates with thrust-augmenting nozzle between baffle plate.The taking-up of magnetoelectricity apparatus for deivation and the putting into when design of active type triphase separator and detachable baffle plate is mainly overhauled the magnetoelectricity apparatus for deivation for ease.
Said magnetoelectricity induces part to be divided into energising carbon-point 7-1 and the pole plate 7-2 part that produces electric field, produces the energising solenoid part 8 in magnetic field.Plate material is high purity graphite plate or conductive metal sheet, and coil method is the insulated type metal wire.Energising carbon-point 7-1 is positioned at energising solenoid 8 centers; Both highly are the 1/3-2/5 of flow lifting type reaction zone 2, and solenoid 8 peripheral diameters are inscribed within flow lifting type reaction zone 2 inwalls, and carbon-point 7-1 diameter is the 1/5-1/6 of solenoid 8 external diameters; Energising pole plate 7-2 is affixed on reaction zone 2 outsides; Height equates highly that with carbon-point 7-1 width equates that with reaction zone 2 outer walls thickness is 3-5mm.Carbon-point 7-1 cooperates generation electric field, solenoid 8 generation magnetic fields with pole plate 7-2.
Said internal recycle whipping appts 9 comprises inhales circulation mixing stirring device and system in the special sinking; Inhaling the circulation mixing stirring device in the sinking can be under system control; Carry out suction with particular flow rate, also can be shifted as required, be mainly used in and make in the reactor drum all mud carry out internal recycle to mix; Thereby make most mud accept the magnetoelectricity inducing action, and reduce sneaking into of oxygen as far as possible.
The present invention constitutes with synthetic glass, and during use, waste water is got into by bottom water inlet pipe 10; Get into flow lifting type reaction zone 2 through water inlet buffer zone 1; After anaerobic ammonia oxidizing bacteria contact reacts in buffer zone 1 and the reaction zone 2, the three-phase separation area of flowing through flows out from rising pipe 11 at last.Anaerobic ammonia oxidizing bacteria receives the stirring of internal recycle whipping appts 9 in buffer zone 1, roll, mix with reaction zone 2 bottom mud, accepts the effect of magnetoelectricity apparatus for deivation.Periphery water-bath part 5 is the water bath heat preservation covers that are used to keep the reactor cell temperature, can effectively control the mud temperature environment.
Magnetoelectricity induced reaction pond body can be used for activating the anaerobic ammonia oxidizing bacteria of active reduction or inactivation; Can study the active condition of magnetic field, electric field or the magnetoelectricity field anaerobic ammonia oxidizing bacteria under acting on simultaneously intuitively, contrast, help exploring the parameters of reactor drum operation with anaerobic ammonia oxidizing bacteria under the normal state; As the righttest voltage reach; The galvanic action scope, the frequency effect scope is to obtain " the righttest effect window " of each parameter; For reactor drum provides condition in the operation of the best use of window, guarantee activation efficiency to greatest extent; And make under the same terms such as temperature, influent quality, the comparative study magnetoelectricity induces the difference on bacterial classification and the non-inducing culture bacterial classification Physiology and biochemistry activity to become possibility.
Through changing energising carbon-point 7-1-pole plate 7-2, the stereo directional of energising solenoid 8 can have following several kinds and induce mode according to the mode of action and the direction in electric field, magnetic field behind the reactor start-up:
1, independent electric field action, about the radial effect direction can be divided into, all around, each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
2, independent the action of a magnetic field, about the radial effect direction can be divided into, all around, each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
3, electric field, magnetic field acts on simultaneously, radial direction: electric field is a fore-and-aft direction, and magnetic field is fore-and-aft direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
4, electric field, magnetic field acts on simultaneously, radial direction: electric field is a fore-and-aft direction, and magnetic field is left and right directions, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
5, electric field, magnetic field acts on simultaneously, radial direction: electric field is a fore-and-aft direction, and magnetic field is above-below direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
6, electric field, magnetic field acts on simultaneously, radial direction: electric field is a left and right directions, and magnetic field is fore-and-aft direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
7, electric field, magnetic field acts on simultaneously, radial direction: electric field is a left and right directions, and magnetic field is left and right directions, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
8, electric field, magnetic field acts on simultaneously, radial direction: electric field is a left and right directions, and magnetic field is above-below direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
9, electric field, magnetic field acts on simultaneously, radial direction: electric field is an above-below direction, and magnetic field is fore-and-aft direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
10, electric field, magnetic field acts on simultaneously, radial direction: electric field is an above-below direction, and magnetic field is left and right directions, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously;
11, electric field, magnetic field acts on simultaneously, radial direction: electric field is an above-below direction, and magnetic field is above-below direction, and each direction all has forward and reverse two kinds of embodiments of electric current or magnetic field simultaneously.

Claims (5)

1. a magnetoelectricity that is used for the rejuvenation bacterium is induced bio-reactor; Comprise reactor body and reactor body water inlet buffer zone, flow lifting type reaction chamber, three-phase separation area, periphery water bath heat preservation cover from bottom to top; It is characterized in that: three-phase separation area comprises one-level three-phase separation area and secondary three-phase separation area; In reactor body, also be provided with magnetic field and produce system and electric field generation system; Also be provided with the internal recycle mixing and stirring system at the bottom of the pond, magnetic field produces system and links to each other with system with electric field generation system.
2. bio-reactor according to claim 1; It is characterized in that: the tripping device of the one-level three-phase separation area of described three-phase separation area is an active type; Its underpart is nested in flow lifting type reaction zone inwall; Two ears are extended on top, and back-off can separate with reactor body when needing in the inwall of secondary three-phase separation area lower end; Totally 6 on secondary three-phase separation area set inside baffle plate, baffle plate is an active type, is fixed on the secondary triphase separator with draw-in groove, and the number of internal baffle can reduce or increase in case of necessity, and subregion communicates with thrust-augmenting nozzle between baffle plate.
3. bio-reactor according to claim 1 is characterized in that: described electric field generation system comprise be contained in the reaction zone bottom relatively to energising carbon-point and pole plate, feed galvanic current stream during use, produce stable radial pattern electric field; Through changing size of current, can produce the electric field of varying strength; Through changing pole plate and carbon-point direction, can produce the electric field of different directions, electric field generation system links to each other with system, external system can freely be controlled the power of strength of electric field, and the time of electric field action, in proper order, mode.
4. bio-reactor according to claim 1; It is characterized in that: described magnetic field generation system comprise be loaded on the reaction zone bottom another relatively to or the energising solenoid of upward and downward; Feed galvanic current stream during use, coil inside produces stable uniform magnetic field; Through changing size of current, the magnetic field that can produce varying strength; Through changing energising solenoid direction, the magnetic field that can produce different directions, magnetic field produce system and link to each other with system, the power that external system can free controlling magnetic field intensity, and the time of the action of a magnetic field, in proper order, mode.
5. bio-reactor according to claim 1 is characterized in that: at the bottom of the described pond internal recycle whipping appts be through be submerged at the bottom of the pond internal-suction type internal recycle whipping appts to mud mixture suction, comprise location suction and portable suction.
CN2012101657740A 2012-05-25 2012-05-25 Magnetoelectric induction bioreactor for rejuvenating bacteria Pending CN102674543A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012101657740A CN102674543A (en) 2012-05-25 2012-05-25 Magnetoelectric induction bioreactor for rejuvenating bacteria

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012101657740A CN102674543A (en) 2012-05-25 2012-05-25 Magnetoelectric induction bioreactor for rejuvenating bacteria

Publications (1)

Publication Number Publication Date
CN102674543A true CN102674543A (en) 2012-09-19

Family

ID=46807185

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2012101657740A Pending CN102674543A (en) 2012-05-25 2012-05-25 Magnetoelectric induction bioreactor for rejuvenating bacteria

Country Status (1)

Country Link
CN (1) CN102674543A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUA20164613A1 (en) * 2016-06-24 2017-12-24 Claudio Poggi Process and related device based on the use of Electromagnetic Field to make the fermentation process more efficient, rapid and repeatable, through the action of micro-organisms, in food or beverages or other liquids for human, animal, agricultural and depolluting use.
CN109665685A (en) * 2019-02-25 2019-04-23 长安大学 A kind of electromagnetism activated sludge reactor and application method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2732729Y (en) * 2004-09-01 2005-10-12 帕克环保技术(上海)有限公司 Internal-circulation anaerobic reactor
JP4162234B2 (en) * 2001-11-15 2008-10-08 株式会社荏原製作所 Anaerobic treatment equipment
CN101928066A (en) * 2010-01-30 2010-12-29 大连理工大学 Electrically enhanced internal anaerobic zero-valent iron reactor
CN102336472A (en) * 2011-09-08 2012-02-01 大连理工大学 Electrically enhanced ANAMMOX biologic nitrogen removal method
CN202576087U (en) * 2012-05-25 2012-12-05 桂林电子科技大学 Bioreactor adopting magnetoelectric induction

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4162234B2 (en) * 2001-11-15 2008-10-08 株式会社荏原製作所 Anaerobic treatment equipment
CN2732729Y (en) * 2004-09-01 2005-10-12 帕克环保技术(上海)有限公司 Internal-circulation anaerobic reactor
CN101928066A (en) * 2010-01-30 2010-12-29 大连理工大学 Electrically enhanced internal anaerobic zero-valent iron reactor
CN102336472A (en) * 2011-09-08 2012-02-01 大连理工大学 Electrically enhanced ANAMMOX biologic nitrogen removal method
CN202576087U (en) * 2012-05-25 2012-12-05 桂林电子科技大学 Bioreactor adopting magnetoelectric induction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘思彤: "电/磁场强化厌氧氨氧化及多菌群协同自养生物脱氮", 《中国博士学位论文全文数据库工程科技Ⅰ辑》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUA20164613A1 (en) * 2016-06-24 2017-12-24 Claudio Poggi Process and related device based on the use of Electromagnetic Field to make the fermentation process more efficient, rapid and repeatable, through the action of micro-organisms, in food or beverages or other liquids for human, animal, agricultural and depolluting use.
CN109665685A (en) * 2019-02-25 2019-04-23 长安大学 A kind of electromagnetism activated sludge reactor and application method

Similar Documents

Publication Publication Date Title
Komolafe et al. Biodiesel production from indigenous microalgae grown in wastewater
CN104628090B (en) A kind of bio-electrochemical reactor and the application in degraded Fluoronitrobenzene class waste water thereof
CN102923866A (en) Device for implementing strengthened treatment for sewage through changing magnetic field and sewage treatment method
CN102674529A (en) Method and special device for treating organic wastewater by combination of microbial fuel cell and microalgae culture
CN112919641B (en) Method and treatment device for denitrification and dephosphorization by utilizing microalgae
CN202576087U (en) Bioreactor adopting magnetoelectric induction
Zhang et al. Biological improvement on combined mycelial pellet for aniline treatment by tourmaline in SBR process
CN102897942A (en) Integrated Fenton advanced oxidation system
CN106396246A (en) A comprehensive sewage treatment plant
CN102502946A (en) Method for treating chemical wastewater by utilizing three-dimensional electrode-biological membrane process
Ye et al. The mechanism of enhanced wastewater nitrogen removal by photo-sequencing batch reactors based on comprehensive analysis of system dynamics within a cycle
CN102417272A (en) Coking waste water treatment method and A / O technology apparatus
CN109354188B (en) Device and method for treating sewage by synchronous nitrification and denitrification biomembrane method
CN102674543A (en) Magnetoelectric induction bioreactor for rejuvenating bacteria
CN104556410A (en) Marine ammonia-oxidizing archaea enrichment device capable of automatically controlling dissolved oxygen
CN107585854B (en) Biological aerated filter, construction method thereof and method for treating water by using biological aerated filter constructed by method
CN105948374A (en) Automatic sewage treatment device
CN105800780B (en) A kind of circulation type sulfate type anaerobic ammonia oxidation microbiological reactor
CN202415301U (en) Printing and dyeing wastewater treatment system
CN104310671A (en) Three-dimensional electrode electro-catalytic reactor wastewater treatment method employing intermittent power supply
CN202594897U (en) Integrated Fenton oxidation system
CN105293694B (en) The stack anaerobic treatment equipment for wastewater of built-in biological catalytic electrolysis system and the method that waste water is handled using it
CN109231443A (en) A kind of industrial wastewater anaerobism desulfurizer and technique based on magnetic iron ore reinforcing
CN203007021U (en) In-situ electro-coagulated membrane bioreactor
CN103449612A (en) Reactor used for obtaining high-concentration yeast to quickly remove wastewater COD (chemical oxygen demand)

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20120919