CN102660498A - Hair follicle activating agent containing PRP (Platelet Rich Plasma) and preparation method thereof as well as method and device for activating hair follicle - Google Patents
Hair follicle activating agent containing PRP (Platelet Rich Plasma) and preparation method thereof as well as method and device for activating hair follicle Download PDFInfo
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- CN102660498A CN102660498A CN2012101625608A CN201210162560A CN102660498A CN 102660498 A CN102660498 A CN 102660498A CN 2012101625608 A CN2012101625608 A CN 2012101625608A CN 201210162560 A CN201210162560 A CN 201210162560A CN 102660498 A CN102660498 A CN 102660498A
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Abstract
The invention relates to the field of biology, in particular to a hair follicle activating agent containing PRP (Platelet Rich Plasma) and a preparation method thereof as well as a method and device for activating hair follicle. The hair follicle activating agent containing the PRP comprises platelet-rich plasma; the preparation method comprises the following steps of: A, adding a blood extraction agent into in-vitro blood; B, carrying out centrifugal layering on the blood to which the blood extraction agent is added , the blood is divided into low-concentration platelet-rich plasma layer, a high-concentration platelet-rich plasma layer, a colloid layer and a red blood cell layer ; and C, extracting the high-concentration platelet-rich plasma layer. According to the method for activating the hair follicle, in-vitro hair follicle is cultured by using the high-concentration platelet-rich plasma layer extracted in the step C. The device for activating the hair follicle is matched with the activating method. According to the hair follicle activating agent and the method and device disclosed by the invention, the activation of the activity of the hair follicle can be promoted.
Description
Technical field
The present invention relates to biological field, be specifically related to hair follicle activator of a kind of PRP of containing and preparation method thereof, activate the hair follicle method and apparatus.
Background technology
" FUE " (Follicular Unit Extraction) technology is the revolutionary hair transplant technology of international the next item up.This technology need not be transplanted flap or used suturing skill from the donor district, therefore can avoid the scar of donor areas, and can further shorten time of recovery.To point out that especially " FUE " technology is a kind ofly to obtain the fresh approach of " FU " from hair loss patient donor district through precise and tiny apparatus, also is to the minimum hair transplant operation of hair follicle invasive.This is a kind of hair transplant technology of not operating on, and is called seamless hair transplant technology again, and it need not to extract flap or use suturing skill from rear pillow part.Add the seamless hair transplant of standing grain FUE and adopt specific apparatus to take out single hair follicle from rear pillow part exactly dispersedly, the direction of growth according to hair is planted in the alopecia district then.Because FUE gets to plant and need not operate on, need not to sew up, so just avoided the linear scar in donor district.
But; Exsomatize the in advance processing of hair follicle of this hair transplant Technology Need; Processing to the hair follicle that exsomatizes only is simple isolated ex vivo usually at present; In this treating processes, often the hair follicle cell damage that when hair follicle extracts, has caused is brought bigger damage, the hair follicle that has greatly slackened after handling is effectively survived.
Summary of the invention
The present invention provides a kind of hair follicle activator that contains high density thrombocyte blood plasma (PRP) and preparation method thereof, activates the hair follicle method and apparatus, can solve the problems of the technologies described above.
The invention provides the hair follicle activator of a kind of PRP of containing, comprising: thrombocyte blood plasma.
Said thrombocyte blood plasma is preferably the high density thrombocyte blood plasma after the blood separation.
The present invention also provides a kind of preparation method who extracts the hair follicle activator of above-mentioned any said PRP of containing, comprises the steps:
A. in blood ex-vivo, add the blood extraction agent;
B. the said blood that adds said blood extraction agent is carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells;
C. extract said high density thrombocyte plasma layer.
Among the said step B, centrifugal stratified centrifugal speed is preferably 3000 rev/mins-5000 rev/mins, is preferably 4500 rev/mins; Centrifugation time was preferably 13 minutes-25 minutes, was preferably 20 minutes.
The present invention also provides preparing method's the method for activation hair follicle of the hair follicle activator of a kind of hair follicle activator that utilizes above-mentioned any said PRP of containing and above-mentioned any described PRP of containing, comprises step:
D. the hair follicle that exsomatizes being put into the said high density thrombocyte blood plasma that step C extracts cultivates.
Among the said step D, the culture temperature of said cultivation is preferably 0 ℃-25 ℃, is preferably 4 ℃; Incubation time was preferably 10 minutes-60 minutes, was preferably 15 minutes-20 minutes; Said hair follicle is preferably the rear pillow part hair follicle.
The present invention also provides a kind of device that utilizes the method for above-mentioned any described activation hair follicle, it is characterized in that, comprising:
Extract centrifugal assembly; Be used for adding the blood extraction agent and the said blood that adds said blood extraction agent being carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells in blood ex-vivo;
Extraction assembly is used to extract the said high density thrombocyte plasma layer that obtains after the centrifugal assembly of said extraction is handled;
Cultivate assembly, be used for the said high density thrombocyte plasma layer culture of ex vivo hair follicle that extracts with said extraction assembly.
The centrifugal assembly of said extraction preferably includes: extraction agent adder and sedimentator; Said extraction agent adder is used for adding the blood extraction agent to blood ex-vivo; Said extraction agent adder preferably is provided with scale; Said sedimentator is used for the said blood that has added said blood extraction agent is carried out centrifugal layering; Said sedimentator is provided with the centrifugation time regulation and control module that is used to regulate centrifugation time and/or is used to regulate the centrifugal speed regulation and control module of centrifugal speed;
And/or,
Said extraction assembly preferably includes: suction pipe, said suction pipe are preferably and are provided with scale; And/or,
Said cultivation assembly preferably includes: incubator, said incubator preferably are provided with incubation time regulation and control module, to regulate incubation time; Also preferably be provided with culture temperature regulation and control module, to regulate culture temperature.
Said device preferably further comprises: automatic control unit, it links to each other with the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly respectively, is used for the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly are controlled automatically.
Said automatic control unit preferably includes cpu or two-position controller.
Through hair follicle activator that contains PRP provided by the invention and preparation method thereof, activation hair follicle method and apparatus, can reach following beneficial effect:
1. the present invention contains in the hair follicle activator of PRP and contains thrombocyte blood plasma; Through being extracted spinning, the blood that exsomatizes obtains; With the thrombocyte blood plasma culture of ex vivo hair follicle that extracts; Thrombocyte blood plasma is that the growth of hair follicle provides certain nutrition, and the damaging cells in the hair follicle that exsomatizes is repaired, the grown cell of the hair follicle that exsomatizes is stimulated, and promotes the activity of hair follicle to activate; And impel the direct entering vegetative period that needs through the hair follicle nearly about 45% of the phase that comes off, make the survival rate of the hair follicle after the cultivation reach 98.5%.
2. the present invention adopts high density thrombocyte blood plasma that hair follicle is cultivated under 4 ℃ envrionment temperature, and making hair follicle is 2-3 hour from traditional three-dimensional survival time, and directly bringing up to the survival time is 17 hours, has further improved the survival rate of hair follicle.
Description of drawings
In order to be illustrated more clearly in the embodiment of the invention or technical scheme of the prior art; Below will do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art; Obviously, the accompanying drawing in below describing only is some embodiments of the present invention, for those of ordinary skills; Under the prerequisite of not paying creative work, can also obtain other embodiment and accompanying drawing thereof according to these accompanying drawing illustrated embodiments.
Fig. 1 is the schematic flow sheet that activates the method for hair follicle in the one embodiment of the invention;
Fig. 2 is the basic block diagram that activates the device of hair follicle in the another embodiment of the present invention.
Embodiment
Below will combine accompanying drawing that the technical scheme of various embodiments of the present invention is carried out clear, complete description, obviously, described embodiment only is a part of embodiment of the present invention, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills are resulting all other embodiment under the prerequisite of not making creative work, the scope that all belongs to the present invention and protected.
The present invention provides the hair follicle activator of a kind of PRP of containing, comprising: thrombocyte blood plasma.
The present invention contains in the hair follicle activator of PRP and contains thrombocyte blood plasma; Through being extracted spinning, the blood that exsomatizes obtains; With the thrombocyte blood plasma culture of ex vivo hair follicle that extracts; Thrombocyte blood plasma is that the growth of hair follicle provides certain nutrition, and the damaging cells in the hair follicle that exsomatizes is repaired, the grown cell of the hair follicle that exsomatizes is stimulated, and promotes hair follicle; And impel the direct entering vegetative period that needs through the hair follicle nearly about 45% of the phase that comes off, make the survival rate of the hair follicle after the cultivation reach 98.5%.Annotate: PRR is a high density thrombocyte blood plasma.
Because the layering of lower concentration thrombocyte blood plasma and high density thrombocyte blood plasma can appear in blood in separation; And high density thrombocyte blood plasma (PRP) is far superior to lower concentration thrombocyte blood plasma to the culture effect of hair follicle; Therefore said thrombocyte blood plasma is preferably the high density thrombocyte blood plasma after the blood separation; High density is generally more than 90%, and lower concentration is generally below 10%.
For above-mentioned thrombocyte blood plasma better is described, the present invention also provides a kind of preparation method who extracts the hair follicle activator of above-mentioned any said PRP of containing, comprises the steps:
A. in blood ex-vivo, add the blood extraction agent;
B. the said blood that adds said blood extraction agent is carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells;
C. extract said high density thrombocyte plasma layer.
Wherein, In order to promote the better centrifugal layering of blood, at first need in blood ex-vivo, add the blood extraction agent, this blood extraction agent can be selected existing any blood extraction agent for use; Selection principle only is to carry out follow-up centrifugal layering, obtains follow-up the layered effect that will obtain.
Need to prove that stripped blood preferably comes from by the hair transplant person.
Among the said step B; Centrifugal speed adopts high speed centrifugation; Centrifugal stratified centrifugal speed is 3000 rev/mins-5000 rev/mins; Such as: 3000 rev/mins, 3500 rev/mins, 4000 rev/mins, 4500 rev/mins, 5000 rev/mins or wherein any two the value between scope, be preferably 4500 rev/mins; Centrifugation time can define through centrifugal effect, and centrifugation time is 13 minutes-25 minutes, such as: 13 minutes, 15 minutes, 18 minutes, 20 minutes, 23 minutes, 25 minutes or wherein any two the value between scope, be preferably 20 minutes.
In conjunction with the equipment that this step can adopt, to recommend to use blood bank's special centrifugal machine, this whizzer can satisfy top rotating speed requirement, reaches centrifugal blood effect preferably.
Such as: TD500 blood bank special centrifugal machine; Maximum speed: 5000 rev/mins; Peak capacity: 15 milliliters * 12; In the operating process; 4500 rev/mins of maximum speeds, Max RCF 3555 * g angle rotor 15 milliliters * 12 (5000 rev/mins) the shortest pick-up period≤5 of RCF:3555 * g second rotary speed precision ± 50 rev/mins, timing range 0～99 minute is centrifugal in short-term.
Extraction among the said step C is promptly directly come out the high density thrombocyte plasma separation after the layering; Can adopt suction pipe absorption mode or the liquid above the high density thrombocyte blood plasma to outwell or sop up; The mode that lower liquid is missed or other modes; In a word, the mode of extraction does not have limitation, as long as high density thrombocyte blood plasma is effectively extracted.
In order will effectively to use through the hair follicle activator that contains PRP of above-mentioned preparing method's preparation; The present invention also provides preparing method's the method for activation hair follicle of the hair follicle activator of a kind of hair follicle activator that utilizes above-mentioned any said PRP of containing and above-mentioned any described PRP of containing, comprises step:
D. the hair follicle that exsomatizes being put into the said high density thrombocyte blood plasma that above-mentioned steps C extracts cultivates.
Simultaneously, the method for this activation hair follicle can be included the step among the preparation method of the hair follicle activator that contains PRP among one of them embodiment, like Fig. 1, shown in.
Among the said step D, the culture temperature of hair follicle has determined the survival time and the survival rate of hair follicle, in order to improve survival rate; Must prolong the survival time, hair follicle at normal temperatures the survival time shorter, the different survival time can appear in the different culture temperature; Therefore; The culture temperature of said cultivation is 0 ℃-25 ℃, learns that through experiment culture temperature is preferably 4 ℃; Under this temperature, can have 2-3 hour of common stripped survival time directly to bring up to the survival time is about 17 hours.The extraction of incubation time and hair follicle and the implantation of cultured hair follicle have very big relation, therefore will be according to this three's mutual relationship and influence decision incubation time, and incubation time is 10 minutes-60 minutes usually, wherein, is preferably 15 minutes-20 minutes; In this scope, hair follicle can be implanted.Because according to the check of people's physiological condition, the hair of the people's rear pillow part probability that drops basically is less, and its hair follicle is commonly called long-lived hair follicle, and therefore, choosing this part hair follicle is reasonable selection, and therefore, said hair follicle is the rear pillow part hair follicle.
In order to make activation hair follicle method obtain concrete operational applications, the present invention also provides a kind of device that utilizes the method for above-mentioned any described activation hair follicle, like Fig. 2, shown in, comprising:
Extract centrifugal assembly; Be used for adding the blood extraction agent and the said blood that adds said blood extraction agent being carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells in blood ex-vivo;
Extraction assembly is used to extract the said high density thrombocyte plasma layer that obtains after the centrifugal assembly of said extraction is handled;
Cultivate assembly, be used for the said high density thrombocyte plasma layer culture of ex vivo hair follicle that extracts with said extraction assembly.
The centrifugal assembly of said extraction comprises: extraction agent adder and sedimentator; Said extraction agent adder is used for adding the blood extraction agent to blood ex-vivo; Said extraction agent adder preferably is provided with scale; Said sedimentator is used for the said blood that has added said blood extraction agent is carried out centrifugal layering; Said sedimentator is provided with the centrifugation time regulation and control module that is used to regulate centrifugation time and/or is used to regulate the centrifugal speed regulation and control module of centrifugal speed;
And/or,
Said extraction assembly comprises: suction pipe, said suction pipe are preferably and are provided with scale; And/or,
Said cultivation assembly comprises: incubator, said incubator preferably are provided with incubation time regulation and control module, to regulate incubation time; Also preferably be provided with culture temperature regulation and control module, to regulate culture temperature.
Said device further comprises: automatic control unit, it links to each other with the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly respectively, is used for the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly are controlled automatically.
Said automatic control unit comprises cpu or two-position controller.
Through hair follicle activator that contains PRP provided by the invention and preparation method thereof, activation hair follicle method and apparatus, can reach following beneficial effect:
1. the present invention contains in the hair follicle activator of PRP and contains thrombocyte blood plasma; Through being extracted spinning, the blood that exsomatizes obtains; With the thrombocyte blood plasma culture of ex vivo hair follicle that extracts; Thrombocyte blood plasma is that the growth of hair follicle provides certain nutrition, and the damaging cells in the hair follicle that exsomatizes is repaired, the grown cell of the hair follicle that exsomatizes is stimulated, and promotes hair follicle; And impel the direct entering vegetative period that needs through the hair follicle nearly about 45% of the phase that comes off, make the survival rate of the hair follicle after the cultivation reach 98.5%.
2. the present invention adopts high density thrombocyte blood plasma that hair follicle is cultivated under 4 ℃ envrionment temperature, and making hair follicle is 2-3 hour from traditional stripped survival time, and directly bringing up to the survival time is 17 hours, has further improved the survival rate of hair follicle.
Various embodiment provided by the invention can make up with any-mode as required each other, the technical scheme that obtains through this combination, also within the scope of the invention.
Obviously, those skilled in the art can carry out various changes and modification to the present invention and not break away from the spirit and scope of the present invention.Like this, belong within the scope of claim of the present invention and equivalent technologies thereof if of the present invention these are revised with modification, then the present invention also comprises these changes and modification interior.
Claims (10)
1. a hair follicle activator that contains PRP is characterized in that, comprising: thrombocyte blood plasma.
2. the hair follicle activator that contains PRP as claimed in claim 1 is characterized in that, said thrombocyte blood plasma is the high density thrombocyte blood plasma after the blood separation.
3. a preparation method who extracts the hair follicle activator of any said PRP of containing of claim 1-2 is characterized in that, comprises the steps:
A. in blood ex-vivo, add the blood extraction agent;
B. the said blood that adds said blood extraction agent is carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells;
C. extract said high density thrombocyte plasma layer.
4. the preparation method who contains the hair follicle activator of PRP as claimed in claim 3 is characterized in that, among the said step B, centrifugal stratified centrifugal speed is 3000 rev/mins-5000 rev/mins, is preferably 4500 rev/mins; Centrifugation time is 13 minutes-25 minutes, is preferably 20 minutes.
5. the method for the preparing method's of the hair follicle activator of a hair follicle activator that utilizes any said PRP of containing of claim 1-2 and any described PRP of containing of claim 3-4 activation hair follicle is characterized in that, comprises step:
D. the hair follicle that exsomatizes being put into the said high density thrombocyte blood plasma that the step C of claim 3-4 extracts cultivates.
6. the method for activation hair follicle as claimed in claim 5 is characterized in that,
Among the said step D, the culture temperature of said cultivation is 0 ℃-25 ℃, is preferably 4 ℃; Incubation time is 10 minutes-60 minutes, is preferably 15 minutes-20 minutes; Said hair follicle is the rear pillow part hair follicle.
7. a device that utilizes the method for claim 5 or 6 described activation hair follicles is characterized in that, comprising:
Extract centrifugal assembly; Be used for adding the blood extraction agent and the said blood that adds said blood extraction agent being carried out centrifugal layering, this blood is divided into lower concentration thrombocyte plasma layer, high density thrombocyte plasma layer, colloid layer, layer of red blood cells in blood ex-vivo;
Extraction assembly is used to extract the said high density thrombocyte plasma layer that obtains after the centrifugal assembly of said extraction is handled;
Cultivate assembly, be used for the said high density thrombocyte plasma layer culture of ex vivo hair follicle that extracts with said extraction assembly.
8. the device of activation hair follicle as claimed in claim 7 is characterized in that,
The centrifugal assembly of said extraction comprises: extraction agent adder and sedimentator; Said extraction agent adder is used for adding the blood extraction agent to blood ex-vivo; Said extraction agent adder preferably is provided with scale; Said sedimentator is used for the said blood that has added said blood extraction agent is carried out centrifugal layering; Said sedimentator is provided with the centrifugation time regulation and control module that is used to regulate centrifugation time and/or is used to regulate the centrifugal speed regulation and control module of centrifugal speed;
And/or,
Said extraction assembly comprises: suction pipe, said suction pipe are preferably and are provided with scale; And/or,
Said cultivation assembly comprises: incubator, said incubator preferably are provided with incubation time regulation and control module, to regulate incubation time; Also preferably be provided with culture temperature regulation and control module, to regulate culture temperature.
9. like the device of claim 7 or 8 described activation hair follicles; It is characterized in that; Said device further comprises: automatic control unit; It links to each other with the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly respectively, is used for the centrifugal assembly of said extraction, said extraction assembly, said cultivation assembly are controlled automatically.
10. the device of activation hair follicle as claimed in claim 9 is characterized in that, said automatic control unit comprises cpu or two-position controller.
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| CN2012101625608A CN102660498A (en) | 2012-05-23 | 2012-05-23 | Hair follicle activating agent containing PRP (Platelet Rich Plasma) and preparation method thereof as well as method and device for activating hair follicle |
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| CN2012101625608A CN102660498A (en) | 2012-05-23 | 2012-05-23 | Hair follicle activating agent containing PRP (Platelet Rich Plasma) and preparation method thereof as well as method and device for activating hair follicle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114159467A (en) * | 2021-10-20 | 2022-03-11 | 江苏省苏北人民医院 | Red platelet-rich fibrin biological injection material and application thereof |
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| US5178883A (en) * | 1984-11-29 | 1993-01-12 | Regents Of The University Of Minnesota | Method for promoting hair growth |
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| CN202595150U (en) * | 2012-05-23 | 2012-12-12 | 北京雍禾植发技术研究院 | Device for activating hair follicle activity through platelet-rich plasma (PRP) |
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Patent Citations (3)
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| US5178883A (en) * | 1984-11-29 | 1993-01-12 | Regents Of The University Of Minnesota | Method for promoting hair growth |
| US20070258956A1 (en) * | 2006-05-02 | 2007-11-08 | Biomet Manufacturing Corp. | Methods and apparatus for promoting hair growth using adipose cell based therapies |
| CN202595150U (en) * | 2012-05-23 | 2012-12-12 | 北京雍禾植发技术研究院 | Device for activating hair follicle activity through platelet-rich plasma (PRP) |
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Cited By (1)
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| CN114159467A (en) * | 2021-10-20 | 2022-03-11 | 江苏省苏北人民医院 | Red platelet-rich fibrin biological injection material and application thereof |
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Application publication date: 20120912 |