CN102657298A - Body color intensifier for large yellow croaker - Google Patents

Body color intensifier for large yellow croaker Download PDF

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Publication number
CN102657298A
CN102657298A CN2012101788037A CN201210178803A CN102657298A CN 102657298 A CN102657298 A CN 102657298A CN 2012101788037 A CN2012101788037 A CN 2012101788037A CN 201210178803 A CN201210178803 A CN 201210178803A CN 102657298 A CN102657298 A CN 102657298A
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large yellow
yellow croaker
body colour
reinforcing agent
body color
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CN2012101788037A
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CN102657298B (en
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张文兵
易新文
麦康森
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Ocean University of China
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Ocean University of China
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Abstract

The invention relates to a body color intensifier for large yellow croakers. The body color intensifier is prepared from the following components in percentage by weight: 20-45wt% of astaxanthin, 20-45wt% of lutein and 20-45wt% of canthaxanthin. The body color intensifier disclosed by the invention is used as a feed additive used in the artificial compound feed for the large yellow croakers. The additive amount is only 0.01-0.15%, and the body color of the artificially cultured large yellow croakers can be obviously improved after the body color intensifier is used about 8 weeks. Meanwhile, the body color intensifier disclosed by the invention is used together with a large yellow croaker feed prepared by the applicant to obtain a preferable effect.

Description

A kind of large yellow croaker body colour reinforcing agent
Technical field
The invention belongs to the feeding additive aquatic animal field, be specifically related to a kind of body colour reinforcing agent that can effectively improve the cultured large yellow croaker body colour.
Technical background
Large yellow croaker is that three the highest big seawater of Chinese output are propagated one of fish artificially, and 2011 annual productions reach 80,000 tons.Wild large yellow croaker back and side present yellowish-brown, and belly is golden yellow, each fin yellow or lark, lip Chinese red.Shortage large yellow croaker body colour forms necessary and carotenoid that himself can not synthesize in the employed bait of large yellow croaker owing to propagate artificially at present; Cause the large yellow croaker body colour serious degradation of propagating artificially; Body colour is thin out to bleach, and has had a strong impact on quality and the market price of propagating large yellow croaker artificially.The research report of at present relevant large yellow croaker body colour seldom only limit to the research report of curcumin on large yellow croaker, but curcumin is a kind of dyestuff, is not that the fish body colour forms necessary material naturally.Therefore industry is badly in need of providing a kind of and can be significantly improved the body colour reinforcing agent of propagating the large yellow croaker body colour artificially.
Summary of the invention
The purpose of this invention is to provide a kind of large yellow croaker body colour reinforcing agent, can significantly improve the body colour of propagating large yellow croaker artificially, thereby remedy the deficiency of prior art.
A kind of large yellow croaker body colour reinforcing agent includes astaxanthin, lutein and canthaxanthin, and its mass percent composition is respectively: 20-45%, 20-45% and 20-45%.
The mass ratio that above-mentioned body colour reinforcing agent, a kind of preferred group become astaxanthin, lutein and canthaxanthin is 1:1:1.
The application process of above-mentioned large yellow croaker body colour reinforcing agent, the addition mass percent in the large yellow croaker feed is 0.01-0.15%.
Above-mentioned large yellow croaker feeding quality percentage is formed as follows: white fish meal 43%, dregs of beans 21%, wheat flour 20-21%, brewer's yeast 2%, fish oil 7.1%, soybean lecithin 1.5%, multidimensional 2%, many ore deposits 2%, phagostimulant 0.3%, ethoxyquinoline 0.1%, mould inhibitor 0.1%.
Phagostimulant is selected the mixture of glycine and betaine for use, and the mass ratio of glycine and betaine is 1:1.
Mould inhibitor is selected the mixture of calcium propionate and fumaric acid for use, and the mass ratio of calcium propionate and fumaric acid is 1:1.
Body colour reinforcing agent of the present invention uses in the large yellow croaker artifical compound feed as feed addictive, and addition is merely 0.01-0.15%, uses about 8 weeks and just can significantly improve the body colour of propagating large yellow croaker artificially.Simultaneously, when body colour reinforcing agent of the present invention cooperates the large yellow croaker feed of applicant's preparation to use, can obtain better effect.
Description of drawings
Fig. 1: the large yellow croaker skin of abdomen brightness value (L that the present invention detects *);
Fig. 2: the large yellow croaker skin of back brightness value (L that the present invention detects *);
Fig. 3: the large yellow croaker skin of abdomen red value (a that the present invention detects *);
Fig. 4: the large yellow croaker skin of back red value (a that the present invention detects *);
Fig. 5: the yellow value of the large yellow croaker belly that the present invention detects (b *);
Fig. 6: the large yellow croaker back that the present invention detects yellow value (b *);
Fig. 7: the large yellow croaker skin of abdomen carotenoid content that the present invention detects;
Fig. 8: the large yellow croaker skin of back carotenoid content that the present invention detects.
The specific embodiment
Body colour reinforcing agent of the present invention is that astaxanthin, lutein and canthaxanthin is formulated according to applicant's definite proportioning that studies for a long period of time.Astaxanthin, lutein and canthaxanthin can be selected commercially available product for use, for example astaxanthin be DSM N. V. produce to add beautiful element pink, lutein and canthaxanthin select for use the auroxanthin and the intelligence spy of Guangzhou Zhiteqi Biotechnology Co., Ltd's production red.
Below in conjunction with concrete embodiment the component of body colour reinforcing agent of the present invention is described.
Embodiment 1:
Astaxanthin, lutein and canthaxanthin are mixed, and its mass percent is respectively 30%, 30% and 35%, also includes the adhesive of bait in addition.
Embodiment 2:
Astaxanthin, lutein and canthaxanthin are mixed, and its mass percent is respectively 20%, 40%, 40%.
Embodiment 3:
Astaxanthin, lutein and canthaxanthin are mixed, mix by the proportioning of 1:1:1.
With the large yellow croaker feed that do not add body colour reinforcing agent of the present invention as control group; The body colour reinforcing agent of the embodiment of the invention 3 addition by 0.01-0.15% is added in the large yellow croaker feed the same with control group; As the experiment feed, carry out culture experiment with above-mentioned feed.
The same a collection of large yellow croaker of selecting artificial incubation then for use is as the experiment fish, and average weight is 13.56 ± 0.05g.Pick out the specification unanimity, athletic large yellow croaker is put in a suitable place to breed in the seawater floating cage that 6 specifications are 1m * 1m * 1.5m, puts 60 tails in each net cage in a suitable place to breed.3 net cages of every kind of feedstuff feeding, culture experiment carried out for 8 weeks altogether.Culture experiment finishes the back and measures carotenoid content in large yellow croaker body colour value and the skin, and with wild large yellow croaker as reference.
The skin volume colour is measured: the L that adopts the international luminous lighting CIE of the committee (1976) regulation *, a *, b *The colour space is represented large yellow croaker skin body colour, wherein L *The expression brightness value ,-b *The expression blue valve ,+b *The yellow value of expression ,-a *The expression green value ,+a *The expression red value.Use colour difference meter in the 8th night in week 19:30~23:30 carry out body colour and measure, colour difference meter is calibrated instrument with blank before using.Measure the body colour value of large yellow croaker back and skin of abdomen respectively, the back is the zone between dorsal fin leading edge below and the side line top, and belly is that the abdomeinal fin of large yellow croaker belly is to the zone between the anal fin.Each net cage is measured 4 tail fishes at random.
(2) skin carotenoid Determination on content: 5 tail fish dorsals got by each net cage of experiment end back and skin of abdomen is preserved in-20 ℃ of refrigerators, is used for measuring the carotenoid content of skin.Get 0.1000g (being accurate to 0.0001g) skin in test tube, add 10ml ethyl acetate: (1:1, V/V) homogenate pipette supernatant behind the centrifugal 5min of 4000g to ethanol; Extract respectively once with 5ml ethyl acetate and 10ml n-hexane respectively then, concentrate the back to dry up three's supernatant at last with high purity nitrogen.Pigment after drying up is dissolved in the 4ml chromatographically pure acetone (containing 0.05%BHT) mixing, the centrifugal 5min of 10000g.Measure at the 448nm place with honourable photometer.Carotenoid content computing formula=10000*V*A/W/E 1%, 1 cm, wherein V is the volume of extract, ml; A is an absorbance; W is an example weight, g; E 1%, 1 cmBe extinction coefficient.
Adopt SPSS15.0 that data are carried out variance analysis.When difference reaches remarkable, carry out multiple comparative analysis with the Tukey check, level of signifiance P < 0.05.
Experimental result shows: as shown in Figure 1, and experimental group large yellow croaker skin of abdomen brightness value and control group there was no significant difference (P>0.05), but be significantly higher than wild fish (P < 0.05).Skin of back brightness value and wild fish there was no significant difference (P>0.05), but be significantly higher than control group (P < 0.05) (Fig. 2).Experimental group large yellow croaker belly red value and control group there was no significant difference (P>0.05), and significantly be lower than wild fish (P < 0.05) (Fig. 3).Experimental group skin of back red value and control group and wild fish there are no significant difference (P>0.05) (Fig. 4).The yellow value of experimental group large yellow croaker skin of abdomen and skin of back is lower than wild fish, but there was no significant difference (P>0.05), and is significantly higher than control group (P < 0.05) (Fig. 5 and Fig. 6).Similar with yellow value, experimental group large yellow croaker skin of back and skin of abdomen pigment content are significantly higher than control group (P < 0.05), are lower than wild group but there was no significant difference (P>0.05) (Fig. 7 and Fig. 8).
Experimental result shows that large yellow croaker body colour reinforcing agent of the present invention can significantly improve the body colour of propagating large yellow croaker artificially, makes it to approach wild large yellow croaker.And the proportioning through between three kinds of compositions can reduce cost effectively, has good market popularization value.
In addition, in order better to embody the effect of body colour reinforcing agent of the present invention, the present invention has also prepared the feed of large yellow croaker specially, includes following component by mass percent: white fish meal 43%; Dregs of beans 21%, wheat flour 20.75-20.9%, brewer's yeast 2%; Fish oil 7.1%, soybean lecithin 1.5%, large yellow croaker is with multidimensional 2%; Large yellow croaker is with many ore deposits 2%, phagostimulant 0.3%, anti-oxidant 0.1%, mould inhibitor 0.1%.
Wherein large yellow croaker can be selected the vitamin of arbitrary money market sale use for use with multidimensional, and its a kind of component is (kg) as follows: Cobastab 1, 25mg; Riboflavin, 45mg; Cobastab 6(puridoxine hydrochloride), 20mg; Cobastab 12, 0.1mg; Vitamin K 3, 10mg; Inositol, 800mg; Cobastab 3(pantothenic acid), 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D 3, 5mg; Vitamin E, 120mg; Vitamin C, 2000mg; Ethoxyquinoline, 150mg; Inferior powder, 16.51g
Large yellow croaker can select for use arbitrary money market sale to use with many ore deposits, and its a kind of composition is (kg) as follows: sodium fluoride, 2mg; KI, 18mg; Cobalt chloride (1 %), 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Manganese sulfate, 60mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 10g; Sodium chloride, 100mg; Zeolite powder, 8.45g.
Phagostimulant is selected the mixture of glycine and betaine for use, and its mass ratio is 1:1.
Mould inhibitor is selected the mixture of calcium propionate and fumaric acid for use, and its mass ratio is 1:1.
The addition of large yellow croaker body colour reinforcing agent prescription of the present invention by 0.01-0.15% joined in this feed, use content of carotenoid in the body colour value that just can significantly improve about 8 weeks in the large yellow croaker skin and the skin, obviously improve the exterior quality of large yellow croaker.Compare with other feed, body colour and the wild large yellow croaker of the large yellow croaker of the body colour reinforcing agent of the present invention of feeding simultaneously are more approaching, make the large yellow croaker of raising that better economic worth arranged.

Claims (6)

1. a large yellow croaker body colour reinforcing agent is characterized in that, includes astaxanthin, lutein and canthaxanthin, and its mass percent composition is respectively: 20-45%, 20-45% and 20-45%.
2. body colour reinforcing agent as claimed in claim 1 is characterized in that being made up of astaxanthin, lutein and canthaxanthin, and the mass ratio of astaxanthin, lutein and canthaxanthin is 1:1:1.
3. the method for using of the described body colour reinforcing agent of claim 1 is to add in the large yellow croaker feed, and the mass percent of interpolation is 0.01-0.15%.
4. the method for using of body colour reinforcing agent as claimed in claim 3 is characterized in that described large yellow croaker feed, and its mass percent is formed as follows: white fish meal 43%; Dregs of beans 21%, wheat flour 20-21%, brewer's yeast 2%; Fish oil 7.1%, soybean lecithin 1.5%, multidimensional 2%; Many ore deposits 2%, phagostimulant 0.3%, ethoxyquinoline 0.1%, mould inhibitor 0.1%.
5. the method for using of body colour reinforcing agent as claimed in claim 3 is characterized in that described phagostimulant is the mixture of glycine and betaine, and wherein the mass ratio of glycine and betaine is 1:1.
6. the method for using of body colour reinforcing agent as claimed in claim 3 is characterized in that described mould inhibitor is the mixture of calcium propionate and fumaric acid, and wherein the mass ratio of calcium propionate and fumaric acid is 1:1.
CN201210178803.7A 2012-06-01 2012-06-01 Body color intensifier for large yellow croaker Expired - Fee Related CN102657298B (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103141673A (en) * 2013-02-04 2013-06-12 上海农好饲料有限公司 Large yellow croaker buoyant puffing feed without fish meal and preparation method thereof
CN104082214A (en) * 2014-07-16 2014-10-08 宁德市富发水产有限公司 Method capable of promoting colors of body surfaces of large yellow croakers to get yellow
CN104757285A (en) * 2015-03-16 2015-07-08 中国水产科学研究院南海水产研究所深圳试验基地 Trachinotus ovatus body color improving compound feed
CN108208472A (en) * 2018-03-21 2018-06-29 集美大学 A kind of feed addictive and rheum officinale fish meal and its preparation method and application
CN108813146A (en) * 2018-05-31 2018-11-16 中国科学院海洋研究所 A kind of Pseudosciaena crocea body colour modifier and preparation method thereof
CN108902566A (en) * 2018-09-18 2018-11-30 中国海洋大学 A kind of rainbow trout culturing feed and its application
CN109090376A (en) * 2018-08-24 2018-12-28 天邦食品股份有限公司 A kind of functional feed for imitating wild cultivation suitable for Larimichthys crocea
CN113678961A (en) * 2021-08-13 2021-11-23 中国海洋大学 Compound feed for improving body color of large yellow croaker and preparation method thereof

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CN101028042A (en) * 2007-02-06 2007-09-05 福建天马饲料有限公司 Slow-settling swelling granular complex feed for large-yellow croaker
CN101411395A (en) * 2008-11-05 2009-04-22 广东恒兴集团有限公司 Feedstuff additive capable of improving body color of aquatic animal
CN101897384A (en) * 2009-05-31 2010-12-01 中国热带农业科学院热带作物品种资源研究所 Method for processing natural plant feed coloring agent

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103141673A (en) * 2013-02-04 2013-06-12 上海农好饲料有限公司 Large yellow croaker buoyant puffing feed without fish meal and preparation method thereof
CN104082214A (en) * 2014-07-16 2014-10-08 宁德市富发水产有限公司 Method capable of promoting colors of body surfaces of large yellow croakers to get yellow
CN104082214B (en) * 2014-07-16 2016-05-11 宁德市富发水产有限公司 A kind of method that can promote large yellow croaker body surface its colour changed into yellow
CN104757285A (en) * 2015-03-16 2015-07-08 中国水产科学研究院南海水产研究所深圳试验基地 Trachinotus ovatus body color improving compound feed
CN108208472A (en) * 2018-03-21 2018-06-29 集美大学 A kind of feed addictive and rheum officinale fish meal and its preparation method and application
CN108208472B (en) * 2018-03-21 2021-07-23 集美大学 Feed additive, large yellow croaker feed and preparation method and application of feed additive and large yellow croaker feed
CN108813146A (en) * 2018-05-31 2018-11-16 中国科学院海洋研究所 A kind of Pseudosciaena crocea body colour modifier and preparation method thereof
CN109090376A (en) * 2018-08-24 2018-12-28 天邦食品股份有限公司 A kind of functional feed for imitating wild cultivation suitable for Larimichthys crocea
CN108902566A (en) * 2018-09-18 2018-11-30 中国海洋大学 A kind of rainbow trout culturing feed and its application
CN113678961A (en) * 2021-08-13 2021-11-23 中国海洋大学 Compound feed for improving body color of large yellow croaker and preparation method thereof

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