CN102640742A - Improved manufacturing method for plant wireworm permanent slide - Google Patents
Improved manufacturing method for plant wireworm permanent slide Download PDFInfo
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- CN102640742A CN102640742A CN2012101175998A CN201210117599A CN102640742A CN 102640742 A CN102640742 A CN 102640742A CN 2012101175998 A CN2012101175998 A CN 2012101175998A CN 201210117599 A CN201210117599 A CN 201210117599A CN 102640742 A CN102640742 A CN 102640742A
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Abstract
The invention relates to an improved manufacturing method for a plant wireworm permanent slide, and belongs to the technical field of plant wireworm specimen manufacture. The manufacturing method comprises the following step of: (1) the kill and fixation of wireworm: using a fixation fluid to thermally kill and meanwhile fix the wireworm; (2) dehydration of the wireworm: adopting a gradient dehydrolyzing method in a dehydration process of the wireworm, and operating each link of the dehydration process of the wireworm under a stereomicroscope; and (3) the manufacture of the wireworm permanent slide: using a piece of tissue paper in a glass slide packing box as an upholder in a tabletting process, and adopting colourless transparent enamel for sealing. The improved manufacturing method provided by the invention has the advantages that the operational process and the required materials are simplified compared with the general wireworm permanent slide tabletting method, the manufacturing difficulty is reduced, the tabletting quality and the success rate are improved, and the manufacturing method is a new method which has the advantages of simplicity in operation, low cost and high efficiency and is used for manufacturing a plant wireworm permanent slide specimen.
Description
Technical field
The invention belongs to Plant nematode preparation of specimen technical field, be specially a kind of improved Plant nematode permanent slide preparation method.
Background technology
Plant nematode belongs to the nematode door in the invertebrate, is one type of nematode that parasitizes positions such as plant roots, stem, leaf, fruit, seed, and it is of a great variety, distribution is extensive, the host is various, harm is serious.According to incompletely statistics, the farm-forestry crop economic loss that causes because of Plant nematode in whole world every year is more than hundreds billion of units.In recent years, frequent day by day along with international trade contacts, pass in and out seedling and plant propagation material thereof increase, and the various plant diseases especially chance propagated of nematodiasis increase greatly, make Plant nematode become the object of countries in the world extensive concern.
In the Plant nematode research work; Permanent slide is made as one of the most classical experimental technique; Can nematode sample long preservation be got off, so that the measurement of further nematode morphosis observation, various data and classification evaluation are especially even more important to the novel species type specimen of identifying.If the slide poor effect, the then follow-up credible result degree that obtains will be had a greatly reduced quality, and the Plant nematode permanent slide of therefore making is that carrying out traditional is the classification on basis and the precondition of identification research with the morphological feature.
At present, the production process of nematode permanent slide sample is comparatively complicated, and concrete steps are following: 1. kill the target nematode: need with add in the target nematode suspension boiling water make temperature be 56-58 ℃ with the heat killed nematode.The target nematode need be alive in this method, and is preferably just isolated from soil or plant tissue, if isolated target nematode was placed for a long time and unfixing or the set time is short, can make the nematode distortion.2. nematode is fixing: after room temperature is lowered the temperature naturally, add TAF fixer (formalin 10ml+ glacial acetic acid 1ml+ glycerine 2ml+ distilled water 87ml) again with fixing nematode form.3. the dehydration of nematode: have lactophenol oil process, glycerine-Ethanol Method and 3 kinds of methods of 4:1FG fixer method comparatively commonly used.The lactophenol oil process is promptly got a clean concave slide, in depression, fills it up with lactophenol oil (phenol 20ml+ lactic acid 20ml+ glycerine 40ml+ distilled water 20m1); Concave slide is placed preheating on the 65-70 ℃ of electric hot plate; Choose the above nematode of fixing 1d that 2-3min gets final product to the lactophenol oil of heat.This method relates to strong toxic chemical phenol in operation, the danger that tool is certain, and sample places under the light and is prone to go to pot.Glycerine-Ethanol Method is about to fixing nematode and chooses in the capsule of 0.5-1ml solution I (95% ethanol 20ml+ glycerine 1ml+ distilled water 79ml); In drier, inject 95% ethanol to device long-pending about 1/10, place above-mentioned capsule drier interior and cover dryer door; Put into 35-40 ℃ of baking oven to drier, shift out behind the 12h; Open dryer door, remove in the capsule after the most solutions, with solution II (glycerine 5ml+95% alcohol 95 ml) add to capsule full till; Capsule is put in the big culture dish, and 40 ℃ of baking oven l2h are in last capsule till the only remaining glycerine.Mainly rely on experience during this normal worm dehydration, be prone to form the too fast nematode torsional deformation that causes of evaporation.4:1FG fixer method is promptly put into 40 ℃ of baking ovens to the fixing capsule of nematode, and lid leaves the slit, lets moisture constantly evaporate; Look the evaporation situation, in time add this fixer of preheating under the equality of temperature, consumption generally accounts for 2/3 of capsule volume; Through constantly " evaporating an interpolation ", moisture evaporates fully in capsule, only retains till a small amount of glycerine.The in operation main dependence experience of this method, and consuming time long, need 5-6 week approximately.4. film-making: use a diameter on alcolhol burner flame, to heat as the clean card punch of 1.5cm; Dip in the paraffin vaseline amalgam that takes a morsel (8 parts of paraffin and 3 parts of vaseline get final product at 65 ℃ of following mixings) immediately; And, be a wax ring after cooling at clean glass slide central authorities flicking; In the wax ring, drip a droplet pure glycerin; Under stereomicroscope with choose pin will be fully the nematode of dehydration move to the central authorities that pure glycerin drips on the slide, evenly distributed and with pin with its end of avaling; Dip in 3 glass fibers approaching with choosing pin, be triangularly arranged, in case sample is shrivelled or flatten polypide at the edge of nematode with the nematode thickness; Get the cover glass of a cleaning, gently baking the several seconds bonnet on the alcolhol burner flame above pure glycerin drips; This slide moved on the 65-70 ℃ of heating plate heat, melt until paraffin vaseline intermixture; Slide is moved to the experimental bench cooling, use mountant mountings such as resinene, gum Arabic again; Treat labelled getting final product after the mountant drying.
Summary of the invention
To the problems referred to above that exist in the prior art, the objective of the invention is to design the technical scheme that a kind of improved Plant nematode permanent slide preparation method is provided, its method is easy, environmental protection, workable.
Described a kind of improved Plant nematode permanent slide preparation method is characterized in that may further comprise the steps:
1) killing with fixing of nematode: the fresh nematode that will separate is chosen in the capsule that fills suitable quantity of water; It is 3-5min that the test tube that fills fixer is placed the beaker water-bath that fills boiling water; Under stereomicroscope with at the bottom of water layer to the ware in the careful sucking-off nematode of the pipettor capsule; Fixer during water intaking is bathed adds rapidly in the capsule, heat killed also fixedly nematode 7-9 days; Described fixer is made up of the following material of volume ratio: 40% formaldehyde 6-10%, pure glycerin 1-5%, distilled water 85-93%;
2) dehydration of nematode: under stereomicroscope, carefully remove fixer with pipettor; In capsule, add an amount of dehydration liquid I; And its uncovered airtight crisper 39-43 ℃ of filling alcohol of placing spent the night, the careful suction gone the dehydration liquid I in the capsule under stereomicroscope, adds dehydration liquid II; Cover the capsule mouth with cover glass, place the airtight crisper 39-43 ℃ of held 2-4h that is added with pure glycerin; Directly in capsule, add 1-2 then and drip pure glycerin; Add cover glass and continue the capping capsule; And capsule placed under airtight crisper 39-43 ℃ that is added with pure glycerin spend the night, remove the cover glass on the capsule, capsule is placed again the airtight crisper 39-43 ℃ of held 4-6h that is added with pure glycerin; The capsule opening moves in the airtight drier, and room temperature held 1-1.5 is after week, and nematode sample wherein promptly can be used for making permanent slide;
Described dehydration liquid I is made up of the following material of volume ratio: 96% ethanol 15-24%, pure glycerin 0.5-2.5%, distilled water 75-84%; Described adding dehydration liquid II is made up of the following material of volume ratio: 96% ethanol 94-96%, pure glycerin 4-6%;
3) making of nematode permanent slide: the clean card punch I end of a diameter 1-2cm is heated to be 8-12s on alcolhol burner flame, is inserted into rapidly in the low melting point cake wax while hot and dips in the paraffin that takes a morsel,, promptly make a wax ring at clean slide central authorities flicking; Collect the slim paper in the slide packing box; And the clean card punch II that uses a diameter to be slightly less than clean card punch I beats and is circular thin paper; According to the size of sample purpose nematode, select circular thin paper number, and with scissors therein the heart cut a little circular port to be placed on the wax ring inner; In circular port, drip a droplet pure glycerin; Under stereomicroscope with choose pin will be fully the nematode of dehydration move to the central authorities that above-mentioned pure glycerin drips, it is sunk to the bottom and evenly distributed; Get the cover glass of a cleaning, gently baking the several seconds bonnet on the alcolhol burner flame above the wax ring; This slide moved on the 65-70 ℃ of heating plate heat, melt until paraffin; Slide is moved to the experimental bench cooling, use water white transparency nail polish mounting again, labelled getting final product.
Described a kind of improved Plant nematode permanent slide preparation method, it is characterized in that in the step 1): the water-bath time is 3.5-4min.
Described a kind of improved Plant nematode permanent slide preparation method, it is characterized in that in the step 1): the volume ratio of each material is in the described fixer: 40% formaldehyde 7-9%, preferred 7.5-8%; Pure glycerin 2-4%, preferred 2.5-3%; Distilled water 87-92%, preferred 89-91%.
Described a kind of improved Plant nematode permanent slide preparation method is characterized in that step 2) in: add that the temperature in the airtight crisper is 40-42 ℃ after the dehydration liquid I; Add that the temperature in the airtight crisper is 40-42 ℃ after the dehydration liquid II, be 2.5-3h standing time; The temperature that is added with then in the airtight crisper of pure glycerin is 40-42 ℃; Remove 40-42 ℃ in the airtight crisper behind the cover glass, standing time 4.5-5h.
Described a kind of improved Plant nematode permanent slide preparation method is characterized in that step 2) in: the volume ratio of each material is in the described dehydration liquid I: 96% ethanol 17-23%, preferred 19-22%; Pure glycerin 0.8-2.2%, preferred 1-1.8%; Distilled water 78-82%, preferred 80-81%.
Described a kind of improved Plant nematode permanent slide preparation method is characterized in that step 2) in: the volume ratio of each material is in the described dehydration liquid II: 96% ethanol 92-97%, preferred 93-95%; Pure glycerin 3-7%, preferred 4-6%.
Described a kind of improved Plant nematode permanent slide preparation method is characterized in that in the step 3): be 9-10s the heat time heating time of clean card punch I, and the heating-up temperature of heating plate is 66-68 ℃.
Described a kind of improved Plant nematode permanent slide preparation method, it is characterized in that in the step 3): described slim paper is interior lining paper or wrapping paper.
Above-mentioned a kind of improved Plant nematode permanent slide preparation method; Its operating process and material requested have been simplified many than conventional nematode permanent slide flaking method; Reduced manufacture difficulty; Improved slice-making quality and success rate, a kind of new method easy and simple to handle, low-cost, high efficiency is provided for making Plant nematode permanent slide sample.
In the present patent application, except other have explanation, other percentage composition is weight percentage.
Embodiment
Below in conjunction with specific embodiment the present invention is described further.
Embodiment 1: a kind of improved Plant nematode permanent slide preparation method may further comprise the steps:
1) killing with fixing of nematode: the fresh nematode that will separate is chosen in the capsule that fills suitable quantity of water, the test tube that fills about 10ml fixer is placed the about 3min of beaker water-bath that fills boiling water; With at the bottom of water layer to the ware in the careful sucking-off nematode of the pipettor capsule, the about 1.5ml of fixer during water intaking is bathed adds rapidly in the capsule under stereomicroscope, and heat killed is also fixedly more than the nematode 7d; Described fixer is made up of the following material of volume ratio: 40% formaldehyde 8ml, pure glycerin 2ml, distilled water 90ml.
2) dehydration of nematode: under stereomicroscope, carefully remove fixer, in capsule, add an amount of dehydration liquid I, and with its uncovered airtight crisper that fills 1/3 volume alcohol that places, 41 ℃ are spent the night with pipettor; The careful suction gone the dehydration liquid I in the capsule under stereomicroscope, covers the capsule mouth with cover glass after the adding dehydration liquid II, places the airtight crisper that is added with pure glycerin, 41 ℃ of held 3h; Directly in capsule, add 1 pure glycerin, add cover glass and continue the capping capsule, and capsule is placed the airtight crisper that is added with pure glycerin, spend the night under 41 ℃; Remove on the capsule cover glass, capsule is placed again the 41 ℃ of held 5h of airtight crisper that are added with pure glycerin; The capsule opening moves in the airtight drier, and the room temperature held is after 1 week, and nematode sample wherein promptly can be used for making permanent slide; Described dehydration liquid I is made up of the following material of volume ratio: 96% ethanol 20ml, pure glycerin 1ml, distilled water 79ml; Described adding dehydration liquid II is made up of the following material of volume ratio: 96% alcohol 95 ml, pure glycerin 5ml.
3) making of nematode permanent slide: about 10s is heated in the clean card punch end of a diameter 1.5cm on alcolhol burner flame, be inserted into rapidly in the low melting point cake wax while hot and dip in the paraffin that takes a morsel,, promptly make a wax ring at clean slide central authorities flicking; Collect the slim paper in the slide packing box; And the clean card punch that uses a diameter to be slightly less than 1.5cm is beaten and is circular thin paper; According to the size of sample purpose nematode, select circular thin paper number, and with scissors therein the heart cut a little circular port to be placed on the wax ring inner; In circular port, drip a droplet pure glycerin; Under stereomicroscope with choose pin will be fully the nematode of dehydration move to the central authorities that above-mentioned pure glycerin drips, it is sunk to the bottom and evenly distributed; Get the cover glass of a cleaning, gently baking the several seconds bonnet on the alcolhol burner flame above the wax ring; This slide moved on 68 ℃ of heating plates heat, melt until paraffin; Slide is moved to the experimental bench cooling, use colourless nail polish mounting again, labelled getting final product.
Above-mentioned steps 1) in: the water-bath time is 3min, 3.5min or 4min.The volume ratio of each material is in the described fixer: 40% formaldehyde 7%, pure glycerin 2%, distilled water 91%; Or 40% formaldehyde 10%, pure glycerin 5%, distilled water 85%; Or 40% formaldehyde 7%, pure glycerin 1%, distilled water 92%;
Above-mentioned steps 2) adds in that the temperature in the airtight crisper is 40 ℃, 42 ℃ or 43 ℃ after the dehydration liquid I; Add that the temperature in the airtight crisper is 40 ℃, 42 ℃ or 43 ℃ after the dehydration liquid II, be 2h or 4h standing time; The temperature that is added with then in the airtight crisper of pure glycerin is 40 ℃, 42 ℃ or 43 ℃; Remove 40 ℃, 42 ℃ or 43 ℃ in the airtight crisper behind the cover glass, standing time 4h or 6h.The volume ratio of each material is in the described dehydration liquid I: 96% ethanol 15%, pure glycerin 2.5%, distilled water 82.5%; Or 96% ethanol 24%, pure glycerin 0.5%, distilled water 75.5%; Or 96% ethanol 18%, pure glycerin 1.5%, distilled water 80.5%.The volume ratio of each material is in the described dehydration liquid II: 96% ethanol 94%, pure glycerin 6%; Or 96% ethanol 96%, pure glycerin 4%.
Above-mentioned steps 3) in: be 8s, 9s, 11s or 12s the heat time heating time of clean card punch I, and the heating-up temperature of heating plate is 65 ℃ or 70 ℃; Placing the slim paper in the wax ring can be micron thin papers such as interior lining paper, wrapping paper, thin paper in the preferred cover glass packing box; Replace mountant sealing slides such as resinene, gum Arabic with the water white transparency nail polish.
Other also can obtain beneficial effect of the present invention all with embodiment 1.
Above-mentioned Plant nematode permanent slide preparation method, from kill, fix, the overall process of dehydration and film-making still need about 20d, but required time of concrete operations is short, only needs 3-4d; Nematode kill with fixation procedure in selected fixer for use, can kill simultaneously and fixing nematode, obviously reduced the usage amount and the operating procedure of reagent; In the dehydration of nematode, adopt the gradient evaporation, nematode is changed in the higher glycerine of concentration step by step, guaranteed the continuity of polypide environment of living in whole film-making process, avoided the distortion of polypide and the distortion of observing effect; In each link to the nematode dehydration; Changing dehydration liquid or dropping glycerine etc. all will operate under stereomicroscope; Can avoid nematode sucking-off with dehydration; Whether have nematode sample generation shrinkage or metaboly, when shrinkage or distortion in various degree took place, the dehydration link that can in time repeat back made the nematode sample that shrinkage takes place be able to a certain extent recover if also can in time check; In the film-making process, do supporter, adopt colourless nail polish mounting with the slim paper instead of glass filament in the slide packing box, can increase the utilization of resources rate, reduce cost of manufacture, improve preparation of specimen's efficient.
Claims (8)
1. improved Plant nematode permanent slide preparation method is characterized in that may further comprise the steps:
1) killing with fixing of nematode: the fresh nematode that will separate is chosen in the capsule that fills suitable quantity of water; It is 3-5min that the test tube that fills fixer is placed the beaker water-bath that fills boiling water; Under stereomicroscope with at the bottom of water layer to the ware in the careful sucking-off nematode of the pipettor capsule; Fixer during water intaking is bathed adds rapidly in the capsule, heat killed also fixedly nematode 7-9 days; Described fixer is made up of the following material of volume ratio: 40% formaldehyde 6-10%, pure glycerin 1-5%, distilled water 85-93%;
2) dehydration of nematode: under stereomicroscope, carefully remove fixer with pipettor; In capsule, add an amount of dehydration liquid I; And its uncovered airtight crisper 39-43 ℃ of filling alcohol of placing spent the night, the careful suction gone the dehydration liquid I in the capsule under stereomicroscope, adds dehydration liquid II; Cover the capsule mouth with cover glass, place the airtight crisper 39-43 ℃ of held 2-4h that is added with pure glycerin; Directly in capsule, add 1-2 then and drip pure glycerin; Add cover glass and continue the capping capsule; And capsule placed under airtight crisper 39-43 ℃ that is added with pure glycerin spend the night, remove the cover glass on the capsule, capsule is placed again the airtight crisper 39-43 ℃ of held 4-6h that is added with pure glycerin; The capsule opening moves in the airtight drier, and room temperature held 1-1.5 is after week, and nematode sample wherein promptly can be used for making permanent slide;
Described dehydration liquid I is made up of the following material of volume ratio: 96% ethanol 15-24%, pure glycerin 0.5-2.5%, distilled water 75-84%; Described adding dehydration liquid II is made up of the following material of volume ratio: 96% ethanol 94-96%, pure glycerin 4-6%;
3) making of nematode permanent slide: the clean card punch I end of a diameter 1-2cm is heated to be 8-12s on alcolhol burner flame, is inserted into rapidly in the low melting point cake wax while hot and dips in the paraffin that takes a morsel,, promptly make a wax ring at clean slide central authorities flicking; Collect the slim paper in the slide packing box; And the clean card punch II that uses a diameter to be slightly less than clean card punch I beats and is circular thin paper; According to the size of sample purpose nematode, select circular thin paper number, and with scissors therein the heart cut a little circular port to be placed on the wax ring inner; In circular port, drip a droplet pure glycerin; Under stereomicroscope with choose pin will be fully the nematode of dehydration move to the central authorities that above-mentioned pure glycerin drips, it is sunk to the bottom and evenly distributed; Get the cover glass of a cleaning, gently baking the several seconds bonnet on the alcolhol burner flame above the wax ring; This slide moved on the 65-70 ℃ of heating plate heat, melt until paraffin; Slide is moved to the experimental bench cooling, use water white transparency nail polish mounting again, labelled getting final product.
2. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1, it is characterized in that in the step 1): the water-bath time is 3.5-4min.
3. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1, it is characterized in that in the step 1): the volume ratio of each material is in the described fixer: 40% formaldehyde 7-9%, preferred 7.5-8%; Pure glycerin 2-4%, preferred 2.5-3%; Distilled water 87-92%, preferred 89-91%.
4. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1 is characterized in that step 2) in: add that the temperature in the airtight crisper is 40-42 ℃ after the dehydration liquid I; Add that the temperature in the airtight crisper is 40-42 ℃ after the dehydration liquid II, be 2.5-3h standing time; The temperature that is added with then in the airtight crisper of pure glycerin is 40-42 ℃; Remove 40-42 ℃ in the airtight crisper behind the cover glass, standing time 4.5-5h.
5. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1 is characterized in that step 2) in: the volume ratio of each material is in the described dehydration liquid I: 96% ethanol 17-23%, preferred 19-22%; Pure glycerin 0.8-2.2%, preferred 1-1.8%; Distilled water 78-82%, preferred 80-81%.
6. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1 is characterized in that step 2) in: the volume ratio of each material is in the described dehydration liquid II: 96% ethanol 92-97%, preferred 93-95%; Pure glycerin 3-7%, preferred 4-6%.
7. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1 is characterized in that in the step 3): be 9-10s the heat time heating time of clean card punch I, and the heating-up temperature of heating plate is 66-68 ℃.
8. a kind of improved Plant nematode permanent slide preparation method as claimed in claim 1, it is characterized in that in the step 3): described slim paper is interior lining paper or wrapping paper.
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| CN2012101175998A CN102640742A (en) | 2012-04-20 | 2012-04-20 | Improved manufacturing method for plant wireworm permanent slide |
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| CN2012101175998A CN102640742A (en) | 2012-04-20 | 2012-04-20 | Improved manufacturing method for plant wireworm permanent slide |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416393A (en) * | 2013-07-30 | 2013-12-04 | 宁波检验检疫科学技术研究院 | Plant nematode temporary glass slide preparation method |
| CN104082275A (en) * | 2014-07-02 | 2014-10-08 | 青岛农业大学 | Preparation technology of aquatic animal embryo microscope slide specimen |
| CN104390840A (en) * | 2014-11-14 | 2015-03-04 | 吴福中 | Device for producing glass slide specimen |
| CN106614520A (en) * | 2016-11-29 | 2017-05-10 | 宁波检验检疫科学技术研究院 | Manufacturing method of plant nematode permanent slide |
| CN108663250A (en) * | 2018-05-14 | 2018-10-16 | 河南林业职业学院 | A kind of coccid slide sample production method |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN109556933A (en) * | 2018-12-12 | 2019-04-02 | 宁波中盛产品检测有限公司 | Root-knot nematode perineal pattern High-speed for preparing Slides |
| CN111157312A (en) * | 2020-01-08 | 2020-05-15 | 中华人民共和国京唐港海关 | Method for manufacturing permanent fungus slide specimen |
| CN115152738A (en) * | 2022-08-04 | 2022-10-11 | 宁波海关技术中心 | Quick sealing piece propping agent for plant nematodes |
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| CN101305709A (en) * | 2008-06-13 | 2008-11-19 | 云南农业大学 | Application of micrometre flimsy in making the nematode permanent glassslide |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416393A (en) * | 2013-07-30 | 2013-12-04 | 宁波检验检疫科学技术研究院 | Plant nematode temporary glass slide preparation method |
| CN104082275A (en) * | 2014-07-02 | 2014-10-08 | 青岛农业大学 | Preparation technology of aquatic animal embryo microscope slide specimen |
| CN104082275B (en) * | 2014-07-02 | 2016-03-23 | 青岛农业大学 | A kind of aquatic livestock embryo microscope slide sample manufacturing technology |
| CN104390840A (en) * | 2014-11-14 | 2015-03-04 | 吴福中 | Device for producing glass slide specimen |
| CN106614520A (en) * | 2016-11-29 | 2017-05-10 | 宁波检验检疫科学技术研究院 | Manufacturing method of plant nematode permanent slide |
| CN108663250A (en) * | 2018-05-14 | 2018-10-16 | 河南林业职业学院 | A kind of coccid slide sample production method |
| CN109085028A (en) * | 2018-06-07 | 2018-12-25 | 西南大学 | The production method that citrus leaves disease solidifies sample |
| CN109556933A (en) * | 2018-12-12 | 2019-04-02 | 宁波中盛产品检测有限公司 | Root-knot nematode perineal pattern High-speed for preparing Slides |
| CN109556933B (en) * | 2018-12-12 | 2021-08-17 | 宁波中盛产品检测有限公司 | Method for quickly preparing perineal pattern of root-knot nematode |
| CN111157312A (en) * | 2020-01-08 | 2020-05-15 | 中华人民共和国京唐港海关 | Method for manufacturing permanent fungus slide specimen |
| CN115152738A (en) * | 2022-08-04 | 2022-10-11 | 宁波海关技术中心 | Quick sealing piece propping agent for plant nematodes |
| CN115152738B (en) * | 2022-08-04 | 2023-11-17 | 宁波海关技术中心 | Plant nematode quick-sealing propping agent |
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Application publication date: 20120822 |