CN102630830A - Feed for improving immunity of sea cucumber and preparation method thereof - Google Patents
Feed for improving immunity of sea cucumber and preparation method thereof Download PDFInfo
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- CN102630830A CN102630830A CN2012101003883A CN201210100388A CN102630830A CN 102630830 A CN102630830 A CN 102630830A CN 2012101003883 A CN2012101003883 A CN 2012101003883A CN 201210100388 A CN201210100388 A CN 201210100388A CN 102630830 A CN102630830 A CN 102630830A
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Abstract
Disclosed are feed for improving immunity of sea cucumber and a preparation method thereof. The feed comprises, by weight, 30-50% of sea mud, 30-50% of sargassum weed, 3.5-4.5% of soybean meal, 4.5-5.5% of shrimp chaff, 4.5-5.5% of whole wheat flour, 0.4-0.8% of vitamin complex, 0.4-0.8% of composite minerals, 4.0-5.0% of fish meal and 0.5-1.0% of antimicrobial peptide. The preparation method includes: weighing the materials, superfinely grinding the materials into 120-150-mesh powder, mixing the materials, adding hot water at 65 DEG C during mixing, cooking after even mixing, making granulated feed 1mm in diameter, recooking, and drying at 40 DEG C to obtain the feed. Compared with the prior art, the feed and the preparation method have the advantages that the feed has balanced nutrition, tastes good, has no toxic and side effects, and is environment-friendly, pollution-free and low-cost, growth rate of sea cucumber is fast, weight gain rate is high and the like.
Description
Technical field
The present invention relates to a kind of holothurian feed and preparation method.
Background technology
Sea cucumber is as one of the world's eight big treasures, and it is not only precious food, also is famous and precious medicinal material, shows after deliberation, and sea cucumber has the memory of raising, delays the sexual gland aging, prevents artery sclerosis, diabetes and effect such as antitumor.Along with improving constantly and the further development and use of sea cucumber in medical field of living standards of the people, the demand of sea cucumber significantly rises, thereby has promoted the development of holothruian cultures industries greatly.
Stichopus japonicus is top-quality a kind of in the Chinese 20 multiple eating sea cucumbers, and it also is one of aquaculture kind of the tool economic worth of northern China, in recent years; Propagating artificially of stichopus japonicus is flourish; But the disease problem also is on the rise, and becomes the bottleneck of the development of this industry of puzzlement, though antibiotic a large amount of use has reached the purpose of preventing and curing diseases to a great extent; But the negative effect of bringing thus also is conspicuous; Therefore, how to reduce or do not use antibiotic and make and culture object and keep the healthy aquaculture mode of higher premunition to become a kind of new concept of modern holothruian cultures industry, well-known; Antibacterial peptide is a kind of ancient bioactive molecule; As the important composition composition of host defense system, host's originality of antibacterial peptide, innate immunity activity, broad spectrum antibiotic activity and characteristics such as molecular mass is little, mechanism of action uniqueness give its traditional antibiotic not available plurality of advantages.
Summary of the invention
The object of the present invention is to provide a kind of not only balanced in nutritionly, mouthfeel is good, has no side effect, and is environment friendly and pollution-free, and cost is low, and the sea cucumber growth rate is fast, and rate of body weight gain is high, a kind of feed that improves sea cucumber immunity that immunity and anti-infection ability are strong and preparation method thereof.
A kind of feed that improves sea cucumber immunity that the present invention proposes comprises following component, each component by weight:
Ooze 30～50%, sargassum 30～50%, dregs of beans 3.5～4.5%, shrimp chaff 4.5～5.5%, wholemeal 4.5～5.5%, B B-complex 0.4～0.8%, composite mineral matter 0.4～0.8%, fish meal 4.0～5.0%, antibacterial peptide 0.5～1.0%.
Said a kind of feed that improves sea cucumber immunity is composed of the following components, each component by weight: ooze 40%, sargassum 40%, dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%, composite mineral matter 0.5%, fish meal 4.5%, antibacterial peptide 0.5%.
Said a kind of feed that improves sea cucumber immunity is composed of the following components, each component by weight: ooze 50%, sargassum 30%, dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%, composite mineral matter 0.5%, fish meal 4%, antibacterial peptide 1%.
A kind of feed that improves sea cucumber immunity that the present invention proposes, its preparation method is following:
Take by weighing each raw material by said components by weight percent, and, each raw material is mixed each raw material ultramicro grinding to 120～150 orders; In mixed process, add an amount of 65 ℃ hot water; Carry out first slaking after mixing, the pellet that diameter is 1mm is processed in granulation then; 60 ℃ of air blast 20min slakings once more, 40 ℃ of dryings obtain the finished particle feed at last then.Above-mentioned mixed process is carried out in the three function mixers at a high speed at B10G; First slaking is that the mode of hot water with spraying joined in the raw material; The raw material that mixes utilizes JZL type Squeezinggranulator to carry out granulation through after the first slaking, and maturing process carries out in air dry oven once more; After the slaking that feed is dry once more, sealing refrigeration.
Among the above-mentioned preparation method, raw material is through twice maturing process, and one is to use 65 ℃ hot water to add with the form of spraying, carries out slaking, and the 2nd, slaking once more in baking oven helps improving the palatability of feed like this.
The holothurian feed that the present invention proposes is balanced in nutrition, comprehensive, good palatability, the sea cucumber of feeding; Fast growth, rate of body weight gain is high, can improve immunity and the anti-Vibrio splindidus infection ability of sea cucumber; The present invention simultaneously adds antibacterial peptide targetedly, and rate of body weight gain can improve more than 7%, and specific growth rate improves more than 10%; Not only shorten the culture-cycle of sea cucumber greatly, and promoted the significantly raising of holothruian cultures efficient; In addition; Toxic and side effect and medicament residue that holothurian feed of the present invention can avoid in the holothruian cultures process using chemical agent such as antibiotic to cause, the sea cucumber death rate can reduce by 57.23%, and immune protective rate has reached 58.82%; So not only help lend some impetus to the sound development of holothruian cultures industry; And promote the commercial value of culturing sea cucumber comprehensively, and the part fish meal in the alternative feed of antibacterial peptide, aquaculture cost practiced thrift greatly.
Description of drawingsThe present invention does not have accompanying drawing.
The specific embodimentEmbodiment 1: a kind of feed that improves sea cucumber immunity is composed of the following components, each component by weight: ooze 40%, sargassum 40%, dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%, composite mineral matter 0.5%, fish meal 4.5%, antibacterial peptide 0.5%; Take by weighing each raw material by said components by weight percent, and, each raw material is mixed each raw material ultramicro grinding to 130 order; In mixed process, add an amount of 65 ℃ hot water; Carry out first slaking after mixing, the pellet that diameter is 1mm is processed in granulation then; 60 ℃ of air blast 20min slakings once more, 40 ℃ of dryings obtain the finished particle feed at last then.
Embodiment 2: a kind of feed that improves sea cucumber immunity is composed of the following components, each component by weight: ooze 50%, sargassum 30%, dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%, composite mineral matter 0.5%, fish meal 4%, antibacterial peptide 1%; Take by weighing each raw material by said components by weight percent, and, each raw material is mixed each raw material ultramicro grinding to 140 order; In mixed process, add an amount of 65 ℃ hot water; Carry out first slaking after mixing, the pellet that diameter is 1mm is processed in granulation then; 60 ℃ of air blast 20min slakings once more, 40 ℃ of dryings obtain the finished particle feed at last then.
Embodiment 3: a kind of feed that improves sea cucumber immunity, composed of the following components, each component by weight: ooze 30%, sargassum 50%, dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%, composite mineral matter 0.5%, fish meal 4.3%, antibacterial peptide 0.7%; Take by weighing each raw material by said components by weight percent, and, each raw material is mixed each raw material ultramicro grinding to 120 order; In mixed process, add an amount of 65 ℃ hot water; Carry out first slaking after mixing, the pellet that diameter is 1mm is processed in granulation then; 60 ℃ of air blast 20min slakings once more, 40 ℃ of dryings obtain the finished particle feed at last then.
Test Example: choose the foregoing description 1 and carry out relevant experiment as follows with the feed of embodiment 2:
Test place: Dalian Ocean University;
Test period: totally 8 weeks experiment, growth experiment was 6 weeks, attacking the poison experiment was 2 weeks;
Test explanation: under identical cultivating condition, identical management condition;
Test group 1: the feed that uses the foregoing description 1 to make, directly throw something and feed and culture sea cucumber;
Test group 2: the feed that uses the foregoing description 2 to make, directly throw something and feed and culture sea cucumber;
Control group: use the market purchasing feed, directly throw something and feed and culture sea cucumber;
Table 1 growth experiment
Table 2 immune indexes
Immune indexes after table 3 challenge test finishes
Table 4 is attacked poison experiment cumulative mortality and immune protective rate
Culture experiment through 6 weeks can find out that holothurian feed of the present invention is balanced in nutrition, comprehensive, good palatability; The sea cucumber of feeding, fast growth, rate of body weight gain is high; Can improve immunity and the anti-Vibrio splindidus infection ability of sea cucumber, the present invention simultaneously adds antibacterial peptide targetedly, compares with the control group holothurian feed; Rate of body weight gain can improve more than 7%, and specific growth rate has improved more than 10%.Lysozyme, acid phosphatase, alkaline phosphatase and the nitricoxide synthase of test group 1 sea cucumber all is significantly increased (P < 0.05), improved 46.06%, 280.85% and 46.30% respectively; After the Vibrio splindidus challenge test finished, acid phosphatase, nitricoxide synthase, the peroxidase of test group 1 sea cucumber all were significantly increased, and have improved 74.23%, 612.00%, 171.04% respectively; Challenge test shows, compares with control group, and test group 1 sea cucumber cumulative mortality has reduced by 57.23%, and immune protective rate is the highest with experimental group 1, reaches more than 58.82 %.Above result verification feed of the present invention have market prospects preferably, can not only promote sea cucumber growth, and can also improve immunity and the anti-Vibrio splindidus infection ability of sea cucumber, be a kind of green feed that can improve sea cucumber immunity and disease-resistant double effects.
Claims (5)
1. feed that improves sea cucumber immunity is characterized in that: comprise following component, each component by weight: ooze 30～50%; Sargassum 30～50%, dregs of beans 3.5～4.5%, shrimp chaff 4.5～5.5%; Wholemeal 4.5～5.5%, B B-complex 0.4～0.8%, composite mineral matter 0.4～0.8%; Fish meal 4.0～5.0%, antibacterial peptide 0.5～1.0%.
2. a kind of feed that improves sea cucumber immunity according to claim 1 is characterized in that: composed of the following components, each component by weight: ooze 40%, sargassum 40%; Dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%; Composite mineral matter 0.5%, fish meal 4.5%, antibacterial peptide 0.5%.
3. a kind of feed that improves sea cucumber immunity according to claim 1 is characterized in that: composed of the following components, each component by weight: ooze 50%, sargassum 30%; Dregs of beans 4%, shrimp chaff 5%, wholemeal 5%, B B-complex 0.5%; Composite mineral matter 0.5%, fish meal 4%, antibacterial peptide 1%.
4. preparation method who improves the feed of sea cucumber immunity, it is characterized in that: component takes by weighing each raw material by weight, and with each raw material ultramicro grinding to 120～150 orders; Each raw material is mixed, in mixed process, add an amount of 65 ℃ hot water, carry out first slaking after mixing; The pellet that diameter is 1mm is processed in granulation then, then 60 ℃ of air blast 20min slakings once more; 40 ℃ of dryings obtain the finished particle feed at last, sealing refrigeration.
5. a kind of preparation method who improves the feed of sea cucumber immunity according to claim 4; It is characterized in that: mixed process is carried out in the three function mixers at a high speed at B10G, and first slaking is that the mode of hot water with spraying joined in the raw material, and the raw material that mixes is after the first slaking of process; Utilize JZL type Squeezinggranulator to carry out granulation; Maturing process carries out in air dry oven once more, and is after the slaking that feed is dry once more, sealing refrigeration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101003883A CN102630830A (en) | 2012-04-09 | 2012-04-09 | Feed for improving immunity of sea cucumber and preparation method thereof |
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| CN2012101003883A CN102630830A (en) | 2012-04-09 | 2012-04-09 | Feed for improving immunity of sea cucumber and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103082154A (en) * | 2013-02-07 | 2013-05-08 | 大连海洋大学 | Feed for improving meat quality of sea cucumber |
| CN103549195A (en) * | 2013-10-31 | 2014-02-05 | 辽宁省农业科学院大连生物技术研究所 | Stichopus japonicus feed and preparation method and application thereof |
| CN104738325A (en) * | 2013-12-26 | 2015-07-01 | 王宝燕 | Feed for juvenile sea cucumber |
| CN106213035A (en) * | 2016-07-22 | 2016-12-14 | 大连海洋大学 | A kind of apostichopus japonicus Selenka feed reducing oligochitosan dissolution velocity and preparation method thereof |
| CN111972543A (en) * | 2020-09-23 | 2020-11-24 | 盐城师范学院 | Slow-release pellet feed used in wintering period of south-shifted stichopus japonicus and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579057A (en) * | 2009-06-12 | 2009-11-18 | 中国水产科学研究院黄海水产研究所 | Method for preparing sea cucumber compound feed with additional Enteromorpha |
| CN102232493A (en) * | 2011-07-19 | 2011-11-09 | 青岛胶南市金龙饲料有限公司 | High-efficient sea cucumber feed and preparation method thereof |
-
2012
- 2012-04-09 CN CN2012101003883A patent/CN102630830A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579057A (en) * | 2009-06-12 | 2009-11-18 | 中国水产科学研究院黄海水产研究所 | Method for preparing sea cucumber compound feed with additional Enteromorpha |
| CN102232493A (en) * | 2011-07-19 | 2011-11-09 | 青岛胶南市金龙饲料有限公司 | High-efficient sea cucumber feed and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| 张家国: "水产动物抗菌肽研究的最新进展", 《大连水产学院学报》, vol. 24, 31 December 2009 (2009-12-31), pages 130 - 133 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103082154A (en) * | 2013-02-07 | 2013-05-08 | 大连海洋大学 | Feed for improving meat quality of sea cucumber |
| CN103549195A (en) * | 2013-10-31 | 2014-02-05 | 辽宁省农业科学院大连生物技术研究所 | Stichopus japonicus feed and preparation method and application thereof |
| CN103549195B (en) * | 2013-10-31 | 2016-02-10 | 辽宁省农业科学院大连生物技术研究所 | A kind of extra large apostichopus japonicus Selenka feed and its preparation method and application |
| CN104738325A (en) * | 2013-12-26 | 2015-07-01 | 王宝燕 | Feed for juvenile sea cucumber |
| CN106213035A (en) * | 2016-07-22 | 2016-12-14 | 大连海洋大学 | A kind of apostichopus japonicus Selenka feed reducing oligochitosan dissolution velocity and preparation method thereof |
| CN111972543A (en) * | 2020-09-23 | 2020-11-24 | 盐城师范学院 | Slow-release pellet feed used in wintering period of south-shifted stichopus japonicus and preparation method thereof |
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Application publication date: 20120815 |