CN102630803A - Humanized milk protein and preparation method thereof - Google Patents
Humanized milk protein and preparation method thereof Download PDFInfo
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- CN102630803A CN102630803A CN2012101489425A CN201210148942A CN102630803A CN 102630803 A CN102630803 A CN 102630803A CN 2012101489425 A CN2012101489425 A CN 2012101489425A CN 201210148942 A CN201210148942 A CN 201210148942A CN 102630803 A CN102630803 A CN 102630803A
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Abstract
The invention discloses humanized milk protein and a preparation method thereof. Cow milk is used as a raw material, the cow milk is filtered in different grades through different films for intercepting different molecular weights, concentrated liquid is mixed according to a proportion, and the humanized milk protein can be directly prepared through atomizing and drying. The humanized milk protein can be directly used as a protein raw material to be added into infant formula milk powder and infant food. The humanized milk protein has advantages that ratio of casein and whey protein is close to the level of the breast milk, so that the humanized milk protein is easy to assimilate by infants.
Description
Technical field
The present invention relates to a kind of cow's milk protein and preparation method thereof, be specifically related to a kind of cow's milk protein that can be used for breast milk infants formula milk and food and preparation method thereof.
Background technology
In breast milk proteins matter, casein accounts for 30%, and lactalbumin accounts for 70%, and wherein lactoalbumin accounts for 26%, does not contain lactoglobulin basically.Cow's milk is as the primary raw material of producing baby milk, and the composition of its albumen and breast milk have very big difference, and casein accounts for 80%, and lactalbumin accounts for 20%, and wherein lactoalbumin accounts for 17%, and lactoglobulin accounts for 43%.The milk powder that directly utilizes cow's milk to produce, in human stomach, because the effect of sour and enzyme forms harder gel, digestibility is relatively poor, and contains more lactoglobulin in the milk powder, and lactoglobulin is allergic former material.Neonate and infant's digestion function of stomach and intestine a little less than, too much casein and lactoglobulin not only can cause the decline of baby to protein utilization rate, also cause allergic reaction easily.
Adopt the gradocol membrane technology that cow's milk is handled; Can be according to the difference of Ruzhong protein molecular weight; Albumen to different separates, and concentrate is mixed in proportion, through concentrating and spray-drying; Can directly make albumen and form protein product, can directly apply in baby milk and the food near breast milk.
Summary of the invention
The purpose of this invention is to provide a kind of emulsified milk albumen and preparation method thereof.
Emulsified milk albumen provided by the present invention, its protein is formed, and the ratio of casein and lactalbumin is 40:60 ~ 30:70; The content that lactoglobulin accounts for lactalbumin is less than 5%.
The present invention also provides the preparation method of emulsified milk albumen, and its preparation method may further comprise the steps:
(a) with raw material milk carry out degreasing obtain defatted milk;
(b) skimmed milk must be rich in caseic concentrate through micro-filtration;
(c) micro-filtration is seen through liquid and get lactalbumin through liquid through ultrafiltration;
(d) ultrafiltration is seen through liquid and get the lactoalbumin concentrate through ultrafiltration
(e) with (b) and (d) the gained concentrate mix;
(f) with the sterilization of (e) gained mixed liquor homogeneous;
(g) with the spray-dried emulsified milk albumen that obtains of the mixed liquor after the sterilization.
In the said method, also be included in the said step (a) before the degreasing raw milk is filtered, be preheated to 35 ~ 40 ℃.
In the said method, defatting step described in the said step (a), the fat content of gained skimmed milk should be less than 0.1%.
In the said method, micro-filtration membrane described in the said step (b) adopts ceramic membrane to handle, and membrane aperture is 70 ~ 80nm.Said micro-filtration thickening temperature is 20 ~ 25 ℃, and pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
In the said method, also be included in ultrafiltration in the said step (c) before, utilize citric acid that the pH value that micro-filtration sees through liquid is transferred to 5.0 ~ 5.5.
In the said method, ultra-filtration process described in the said step (c), the material of milipore filter are polyether sulfone, and molecular cut off is 30kDa.Said ultrafiltration thickening temperature is 40 ~ 50 ℃, and ultrafiltration pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
In the said method, ultra-filtration process described in the said step (d), the material of milipore filter are polyether sulfone, and molecular cut off is 10kDa.Said ultrafiltration thickening temperature is 40 ~ 50 ℃, and ultrafiltration pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
In the said method, mixed process described in the said step (e), micro-filtration concentrate and lactoalbumin concentrate are pressed 1.5:10 ~ 1:10 mixed.
In the said method, homogenizing process described in the said step (f), homogenization pressure are 15 ~ 20MPa.
In the said method, sterilization process described in the said step (f), sterilization temperature are 70 ~ 75 ℃, and the time is 15s.
In the said method, also be included in the said step (g) and will obtain mixed liquor before the spray-drying to be concentrated into dry matter content be 40%, carry out spray-drying again.
In the said method, spray-drying adopts vertical spray drying tower in the said step (g), and said spray-drying EAT is 110 ~ 200 ℃, and preferred 120 ~ 150 ℃, leaving air temp is 90 ~ 105 ℃, and atomisation pressure is 0.18 ~ 0.20MPa.
The specific embodiment
Instance 1
Raw material is a fresh raw milk on the same day, 4 ℃ of refrigeration storages down,
After filtering, be preheated to 40 ℃, carry out fat with cream-milk separator and separate, the fat content of gained skimmed milk is 0.06%.
Micro-filtration concentrates used ceramic membrane, and membrane aperture is 80nm, and the micro-filtration temperature is 25 ℃, and micro-filtration pressure is 0.3MPa, and cycles of concentration is 4 times.
Ultrafiltration concentrates micro-filtration and sees through liquid, adopts poly (ether-sulfone) ultrafiltration membrane, and molecular cut off is 30kDa, and the ultrafiltration temperature is 50 ℃, and ultrafiltration pressure is 0.25MPa, and cycles of concentration is 5 times.
The ultrafiltration ultrafiltration concentration sees through liquid, adopts poly (ether-sulfone) ultrafiltration membrane, and molecular cut off is 10kDa, and the ultrafiltration temperature is 50 ℃, and ultrafiltration pressure is 0.25MPa, and cycles of concentration is 5.5.
The micro-filtration concentrate and the second step ultrafiltration concentrate be the 1:10 mixed by volume.
Mixed liquor carries out homogeneous, and homogenization pressure is 18MPa; Carry out sterilization behind the homogeneous, sterilization conditions is 72 ℃, 15s.
The EAT of spray drying tower is 130 ℃, and leaving air temp is 90 ℃, and atomisation pressure is 0.18MPa.Products obtained therefrom is emulsified milk albumen.
Adopt national standard method to analyze its albumen compositions indicator in above-mentioned gained emulsified milk albumen, the result is as shown in table 1:
The albumen of table
emulsified milk albumen is formed
The organoleptic indicator of the above-mentioned emulsified milk albumen that makes:
1. color and luster: the milky of uniformity or have a little yellow;
2. taste smell: have the distinctive milk fragrance of this product, do not have putrid and deteriorated smell;
3. structural state: uniformity Powdered, no-sundries, no block, the visible impurity of no naked eyes.
Claims (9)
1. emulsified milk albumen, its albumen is formed:
The ratio of casein and lactalbumin is 40:60 ~ 30:70; Wherein lactoglobulin content accounts for protein content of whey and is less than 5%.
2. prepare the method for emulsified milk albumen, comprise the steps:
(a) with raw material milk carry out degreasing obtain defatted milk;
(b) skimmed milk must be rich in caseic concentrate through micro-filtration;
(c) micro-filtration is seen through liquid and get lactalbumin through liquid through ultrafiltration;
(d) ultrafiltration is seen through liquid and get the lactoalbumin concentrate through ultrafiltration
(e) with (b) and (d) the gained concentrate mix;
(f) with the sterilization of (e) gained mixed liquor homogeneous;
(g) with the spray-dried emulsified milk albumen that obtains of the mixed liquor after the sterilization.
3. according to the said method of claim 2, it is characterized in that:
Before also being included in degreasing in the step (a) raw milk is filtered, be preheated to 35 ~ 40 ℃, the fat content of gained skimmed milk should be less than 0.1%.
4. according to the said method of claim 2, it is characterized in that:
Micro-filtration membrane described in the step (b) adopts ceramic membrane to handle, and membrane aperture is 70 ~ 80nm, and said micro-filtration thickening temperature is 20 ~ 25 ℃, and pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
5. according to the said method of claim 2, it is characterized in that:
Before also being included in ultrafiltration in the step (c), utilize citric acid that the pH value that micro-filtration sees through liquid is transferred to 5.0 ~ 5.5, said ultra-filtration process, the material of milipore filter are polyether sulfone, and molecular cut off is 30kDa; The ultrafiltration thickening temperature is 40 ~ 50 ℃, and ultrafiltration pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
6. according to the said method of claim 2, it is characterized in that:
Ultra-filtration process described in the step (d), the material of milipore filter are polyether sulfone, and molecular cut off is 10kDa, and the ultrafiltration thickening temperature is 40 ~ 50 ℃, and ultrafiltration pressure is 0.2 ~ 0.3MPa, and cycles of concentration is 4 ~ 5.5 times.
7. according to the said method of claim 2, it is characterized in that:
Mixed process described in the step (e), micro-filtration concentrate and lactoalbumin concentrate are pressed 1.5:10 ~ 1:10 mixed.
8. according to the said method of claim 2, it is characterized in that:
Homogenizing process described in the step (f), homogenization pressure are 15 ~ 20MPa, and said sterilization process, sterilization temperature are 70 ~ 75 ℃, and the time is 15s.
9. according to the said method of claim 2, it is characterized in that:
Also be included in the step (g) and will obtain mixed liquor before the spray-drying to be concentrated into dry matter content be 40%; Carry out spray-drying again; Spray-drying adopts vertical spray drying tower in the step (g), and said spray-drying EAT is 110 ~ 200 ℃, preferred 120 ~ 150 ℃; Leaving air temp is 90 ~ 105 ℃, and atomisation pressure is 0.18 ~ 0.20MPa.
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| CN2012101489425A CN102630803A (en) | 2012-05-15 | 2012-05-15 | Humanized milk protein and preparation method thereof |
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| CN2012101489425A CN102630803A (en) | 2012-05-15 | 2012-05-15 | Humanized milk protein and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783256A (en) * | 2013-12-30 | 2014-05-14 | 东北农业大学 | Method for preparing high-solubility cow milk concentrated protein powder by continuous volume-changing percolation |
| WO2015041529A2 (en) | 2013-09-19 | 2015-03-26 | N.V. Nutricia | Improved process for the humanization of animal skim milk |
| RU2608151C1 (en) * | 2015-12-07 | 2017-01-16 | Дмитрий Геннадьевич Бурцев | Method for production of milk product |
| CN106417888A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein |
| CN106666068A (en) * | 2017-01-12 | 2017-05-17 | 宁夏塞尚乳业有限公司 | Milk protein concentrate produced by membrane treatment technology, and preparation method of milk protein concentrate |
| CN107920541A (en) * | 2015-08-31 | 2018-04-17 | 瓦利奥有限公司 | The preparation method for the dairy products that plasmin activity reduces |
| CN111387292A (en) * | 2020-04-02 | 2020-07-10 | 黑龙江飞鹤乳业有限公司 | Infant formula milk powder base material and preparation method thereof |
| US10993454B2 (en) | 2011-02-18 | 2021-05-04 | Valio Ltd. | Milk-based product and a method for its preparation |
| CN114586847A (en) * | 2022-03-18 | 2022-06-07 | 中国农业科学院农产品加工研究所 | Fresh milk grade mother emulsified milk protein base material and preparation method thereof |
| CN114805547A (en) * | 2022-04-13 | 2022-07-29 | 吉林大学 | Method for preparing alpha-lactalbumin by combining membrane technology with enzyme method |
| CN117121946A (en) * | 2023-08-24 | 2023-11-28 | 安徽天凯生物科技有限公司 | Method and device for preparing mother emulsified infant formula milk powder and functional milk powder |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169666A (en) * | 1991-11-14 | 1992-12-08 | The United States Of America As Represented By The Secretary Of Agriculture | Preparation of simulated human milk protein by low temperature microfiltration |
| WO1994013148A1 (en) * | 1992-12-11 | 1994-06-23 | Immunotec Research Corporation Ltd. | Process for producing an undernatured whey protein concentrate |
| TWI301052B (en) * | 2001-12-21 | 2008-09-21 | Wyeth Corp | Infant formula compositions comprising increased amounts of alpha-lactalbumin |
| CN101491287A (en) * | 2008-01-24 | 2009-07-29 | 滕国新 | Method for extracting lactose and lactoalbumin from whey and producing formulation milk powder |
| CN101686706A (en) * | 2007-04-16 | 2010-03-31 | 菲仕兰产品有限公司 | Functional serum protein product for use in infant food and therapeutic compositions and methods for the preparation thereof |
| CN101926408A (en) * | 2010-02-23 | 2010-12-29 | 北京三元食品股份有限公司 | Method and device for producing casein and whey protein by separating milk |
| WO2011051557A1 (en) * | 2009-10-28 | 2011-05-05 | Valio Ltd | Whey protein product and a method for its preparation |
-
2012
- 2012-05-15 CN CN2012101489425A patent/CN102630803A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5169666A (en) * | 1991-11-14 | 1992-12-08 | The United States Of America As Represented By The Secretary Of Agriculture | Preparation of simulated human milk protein by low temperature microfiltration |
| WO1994013148A1 (en) * | 1992-12-11 | 1994-06-23 | Immunotec Research Corporation Ltd. | Process for producing an undernatured whey protein concentrate |
| TWI301052B (en) * | 2001-12-21 | 2008-09-21 | Wyeth Corp | Infant formula compositions comprising increased amounts of alpha-lactalbumin |
| CN101686706A (en) * | 2007-04-16 | 2010-03-31 | 菲仕兰产品有限公司 | Functional serum protein product for use in infant food and therapeutic compositions and methods for the preparation thereof |
| CN101491287A (en) * | 2008-01-24 | 2009-07-29 | 滕国新 | Method for extracting lactose and lactoalbumin from whey and producing formulation milk powder |
| WO2011051557A1 (en) * | 2009-10-28 | 2011-05-05 | Valio Ltd | Whey protein product and a method for its preparation |
| CN101926408A (en) * | 2010-02-23 | 2010-12-29 | 北京三元食品股份有限公司 | Method and device for producing casein and whey protein by separating milk |
Non-Patent Citations (3)
| Title |
|---|
| 张和平 等: "《乳品工艺学》", 31 January 2007, 中国轻工业出版社 * |
| 张国农 等: "新一代母乳化乳（或乳粉）及其研究概况", 《中国奶牛》 * |
| 汤远会: "《新型母乳化奶粉的研究》", 《食品科学》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10993454B2 (en) | 2011-02-18 | 2021-05-04 | Valio Ltd. | Milk-based product and a method for its preparation |
| WO2015041529A2 (en) | 2013-09-19 | 2015-03-26 | N.V. Nutricia | Improved process for the humanization of animal skim milk |
| CN105848486A (en) * | 2013-09-19 | 2016-08-10 | N·V·努特里奇亚 | Improved process for the humanization of animal skim milk |
| EP3837985A3 (en) * | 2013-09-19 | 2021-09-29 | N.V. Nutricia | Improved process for the humanization of animal skim milk |
| CN103783256A (en) * | 2013-12-30 | 2014-05-14 | 东北农业大学 | Method for preparing high-solubility cow milk concentrated protein powder by continuous volume-changing percolation |
| US11800877B2 (en) | 2015-08-31 | 2023-10-31 | Valio Ltd. | Method for producing a milk-based product with reduced plasmin-activity |
| CN107920541A (en) * | 2015-08-31 | 2018-04-17 | 瓦利奥有限公司 | The preparation method for the dairy products that plasmin activity reduces |
| RU2608151C1 (en) * | 2015-12-07 | 2017-01-16 | Дмитрий Геннадьевич Бурцев | Method for production of milk product |
| CN106417888A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein |
| CN106666068A (en) * | 2017-01-12 | 2017-05-17 | 宁夏塞尚乳业有限公司 | Milk protein concentrate produced by membrane treatment technology, and preparation method of milk protein concentrate |
| CN111387292A (en) * | 2020-04-02 | 2020-07-10 | 黑龙江飞鹤乳业有限公司 | Infant formula milk powder base material and preparation method thereof |
| CN111387292B (en) * | 2020-04-02 | 2023-07-04 | 黑龙江飞鹤乳业有限公司 | Infant formula powder milk base material and preparation method thereof |
| CN114586847A (en) * | 2022-03-18 | 2022-06-07 | 中国农业科学院农产品加工研究所 | Fresh milk grade mother emulsified milk protein base material and preparation method thereof |
| CN114586847B (en) * | 2022-03-18 | 2024-04-26 | 中国农业科学院农产品加工研究所 | Fresh-milk-grade breast milk-like milk protein base material and preparation method thereof |
| CN114805547A (en) * | 2022-04-13 | 2022-07-29 | 吉林大学 | Method for preparing alpha-lactalbumin by combining membrane technology with enzyme method |
| CN117121946A (en) * | 2023-08-24 | 2023-11-28 | 安徽天凯生物科技有限公司 | Method and device for preparing mother emulsified infant formula milk powder and functional milk powder |
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Application publication date: 20120815 |