CN102599336A - Composite aqueous extract of Fu brick tea and application thereof - Google Patents
Composite aqueous extract of Fu brick tea and application thereof Download PDFInfo
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- CN102599336A CN102599336A CN2012100690046A CN201210069004A CN102599336A CN 102599336 A CN102599336 A CN 102599336A CN 2012100690046 A CN2012100690046 A CN 2012100690046A CN 201210069004 A CN201210069004 A CN 201210069004A CN 102599336 A CN102599336 A CN 102599336A
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Abstract
Composite aqueous extract of Fu brick tea is made by steps: breaking Fu brick tea, mixing the broken Fu brick tea with leaves of sweet wormwood and mint, soaking with distilled water, heating under mixing to the temperature of from 70 DEG C to 100 DEG C for 10-30 minutes, allowing the mixture to stand, cooling to room temperature, and filtering. The composite aqueous extract of Fu brick tea can be orally taken to treat bacterial diarrhea according to the degree of diarrhea.
Description
Technical field
The present invention relates to technical field of food biotechnology, refer to a kind of compound Fu brick tea water extract and the effect in preparation treatment bacterial diarrhea medicine thereof especially.
Background technology
The Fu brick tea is claimed " lake tea " in early days, because of in processing on the hot summer days, claims again " volt tea ", and be the black tea that the production and processing cycle is the longest in all teas, technology is the most complicated, unique.The Fu brick tea is a raw material with 3～4 grades of black wool tea, through screening, decatize, wet heap, claim tea, steam tea, compacting, pack, grow dim, operations such as drying, check make with extra care and form." wet heap " is the critical process during the Fu brick tea is produced, and can form the distinctive color, smell and taste of Fu brick tea.Black tea can improve the gastrointestinal function of human body.This has obtained confirmation for a long time among the people; " herbal classic is met the source " claimed: " ginger tea is controlled dysentery---pay no attention to red white, cold and hot use all should ", " close vinegar control rush down dysentery very imitate "; Become extract with old tea (depositing the black tea of decades) infusion; Be aided with ginger splices, brown sugar is drunk, and generally just As the medicine took effect, the symptoms lessened less than two division of day and night.
Antibiotic invention is a human much progress, but along with antibiotic abuse phenomenon is more and more serious, a lot of unavoidable problem occurred, even the superbacteria that no medicine can be cured occurred.People wait in expectation and develop more anti-infective, new antiviral drugs that the resistance to the action of a drug is low, toxic and side effect is little that have more.Tealeaves comes from natural, and the nearly all pathogen of occurring in nature is all had certain inhibition ability, and verified its has the obvious suppression effect to multiple microbial growth, and for different bacteriums, tealeaves shows different fungistatic effects.A lot of scholars utilize the active component such as Tea Polyphenols, the tea saponin etc. that extract in tealeaves or the tealeaves that food borne bacteria, intestinal, source, oral cavity property bacterium, skin source property bacterium etc. are carried out bacteriostatic experiment, have obtained effect preferably.
Summary of the invention
Technical problem to be solved by this invention is: to the deficiency of above-mentioned prior art; A kind of compound Fu brick tea water extract that adopts pure plant extracts to process is provided; This therapeutic action of compound Fu brick tea water extract in bacterial diarrhea also is provided simultaneously; Can reduce antibiotic use, reduce the drug resistance of food-borne pathogenic microorganism.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is: a kind of compound Fu brick tea water extract is the ratio that adds 10g sweet wormwood and 2g dried peppermint leaf in every 288g Fu brick tea; Get the Fu brick tea and break into pieces, mix, add the ratio of 8-30ml distilled water again in every 3g Fu brick tea, sweet wormwood and dried peppermint leaf with sweet wormwood and dried peppermint leaf; Mixed Fu brick tea, sweet wormwood and dried peppermint leaf are soaked in the distilled water, heat while stirring again, to temperature be 70-100 ℃; And in 70-100 ℃ of maintenance 10-30 minute, leave standstill, be cooled to room temperature; Filter, gained tea juice is compound Fu brick tea water extract.
The above-mentioned Fu brick tea of using is meant according to traditional processing technology production and the old old tea stored through 2-10.
The compound Fu brick tea water extract that the present invention makes has therapeutic action in bacterial diarrhea, administering mode is an oral administration.Wherein, bacterial diarrhea is meant by Escherichia coli O 157, the animal diarrhoea that salmonella, staphylococcus aureus or the inferior Salmonella of enteritis Yale cause.
Specify the effect of compound Fu brick tea water extract of the present invention in the treatment bacterial diarrhea with pharmacodynamics test below.
Bacterial diarrhea with due to the water extract treatment Escherichia coli O 157 of the present invention infection is that example describes.
200 of Kunming kind SPF mouse, male and female half and half, body weight 18-22g.Adaptability is divided into 5 groups after feeding at random: dose groups (d group), tea juice high dose group (e group) in normal group (a group), model group (b group), tea juice low dose group (c group), the tea juice; Every group 40; Be 1g/kg/day according to low dose group respectively; Middle dose groups is 10g/kg/day, and high dose group is after the amount of 20g/kg/day is given basic, normal, high dose groups mouse stomach corresponding dosage tea juice 3d, these 3 groups and model group mouse stomach Escherichia coli O 157: the modeling of H7 bacterium liquid; Escherichia coli O 157: H7 is available from Chinese industrial microorganism fungus kind preservation administrative center, and bacterium numbering is 21530.Selection can make mouse infection E.coli O157:H7 but non-lethal dosage for attacking toadstool liquid, attack malicious approach and be per os and irritate stomach, every mouse is attacked malicious OD
600=1.0 bacterium liquid 0.4mL.Attack aseptic the getting of poison back 3h and respectively organize the stool in mice sample, detect E.coli O157 in the ight soil, confirm that attacking malicious mouse infects with E.coli O157 kit.
Regularly disposable give basic, normal, high dose groups mouse stomach corresponding dosage tea juice every day behind the model copy, irritates stomach physiological saline to model group, irritates the stomach amount and only be 0.4mL/, and normal group is left intact.Experimental session is respectively organized mouse and is freely drunk water and search for food, and puts to death behind the 10d continuously.
Get 10 mouse for every group and detect the PBC discovery, the mouse platelets of ehec infection O157:H7 obviously reduces, and irritates the mouse platelets content raising of the middle and high dosage Fu of stomach brick tea, even returns to normal level, and is tangible dose-effect relationship.Blood biochemistry detection to mouse is found, compares with model group, and the mice serum triglycerides of irritating the basic, normal, high dosage tea of stomach juice descends; Irritate the middle and high dosage tea of stomach juice mouse platelets counting and raise, AST, Scr and BUN reduce in the serum; Irritate dosage tea juice total serum protein and albumin rising in the stomach.
Every group of 10 mouse are got gastric tissue, duodenum, ileum, and the sample of colon, process pathological section.Mirror is observed down, can know that after the modeling of model group small white mouse, the gastric tissue pathological change mainly shows as oedema, gastric gland atrophy, cells of superficial layer come off (referring to Fig. 2 b).Nephron damage shows as renal tubular cell necrosis, pathology messangial cell liquefaction and necrosis, vacuolation.Visible neutrophil leucocyte and monocyte infiltration (referring to Fig. 1 b) in the capillary.Duodenum and ileum congestion and edema phenomenon are obvious, and lamina propria and submucosa have tangible inflammation oedema, and inflammatory cell is focal or diffuse infiltrating, and villous fracture, obscission (referring to Fig. 3 b, and Fig. 4 b).Colon intestines wall thickening, mucous membrane are congested down, and enteraden reduces, a large amount of inflammatory cell infiltration to lamina proprias even flesh layer (referring to Fig. 5 b).After irritating the compound Fu brick tea of stomach water extract, irritate food low dosage tea juice group each target organ of mouse damage than the model group mouse take a favorable turn slightly (referring to Fig. 1 c, Fig. 2 c, Fig. 3 c, Fig. 4 c, and Fig. 5 c); The renal tissue of most of mouse of middle and high dose groups is normal, and renal tubule and glomerulus institutional framework are intact.Stomach, intestinal tissue inflammation, edema extinction, duodenum and ileum fine hair are recovered normally basically, have extremely individually the limitation inflammatory reaction (referring to Fig. 1 d-Fig. 1 e, Fig. 2 d-Fig. 2 e, Fig. 3 d-Fig. 3 e, Fig. 4 d-Fig. 4 e, and Fig. 5 d-Fig. 5 e).This shows that the compound Fu brick tea water extract of middle and high dosage can effectively be repaired the pathology damage that Escherichia coli O 157 causes.
Every group of 10 mouse detect immunologic function respectively, and the method for weighing with immune organ detects the immune organ index, and the method for cleaning up with carbon detects the mononuclear macrophage function, detects the serum hemolysin index with the blood clotting method, detects CD4 on the intestinal mucosa with the SABC method
+T cell and CD8
+The expression of T cell.The result shows, compares with model group, and the mouse thymus index of irritating the middle and high dosage tea of stomach juice increases, and the serum hemolysin antibody titer improves, CD4 on the intestinal mucosa
+And CD8
+Developed by molecule shows its CD4
+T cell and CD8
+The T cell quantity increases.Three dosage tea juice group mouse monokaryon-macrophage functions improve.Through proving that from non-specific immune function, humoral immune function and enteron aisle local immunity function three aspect detection model immune function of mice compound Fu brick tea water extract can effectively improve the immunologic function of the model mice of ehec infection O157.
Detecting three aspects from hematology detection, pathology detection and immunologic function all confirms; Fu brick tea compound can effectively be alleviated the infection symptoms of model mice; Protection is because of the impaired liver of infection, renal function; Improve platelet count in the peripheral blood, repair the damage of main target organ such as intestines and stomach and kidney, improve immunologic function.So, compound Fu brick tea water extract of the present invention can recover the impaired hepatic and renal function because of ehec infection O157:H7, improves the peripheral blood platelet count; Can prevent and repair the pathological change of gastrointestinal tract mucosa and kidney, the protection target organ; Improve immunologic function; And can adjust gut flora, help to recover the microecological balance of enteron aisle.
Description of drawings:
Fig. 1 is that microscopically amplifies 40 times ephrosis reason slice map.
Wherein: a representes normal group, b representation model group (the A district shows pathology messangial cell liquefaction and necrosis, vacuolation), and c representes low dose group, dose groups during d representes, e representes high dose group.
Fig. 2 is that microscopically amplifies 10 times stomach trouble reason slice map.
Wherein: a representes normal group, b representation model group (the B district shows the obvious broadening in fundus gland body of gland gap, and oedema is arranged), and c representes low dose group, dose groups during d representes, e representes high dose group.
Fig. 3 is that microscopically amplifies 10 times duodenum pathology slice map.
Wherein: a representes normal group, b representation model group (C district representation model group mouse duodenum congestion and edema phenomenon is obvious, and villous ruptures, comes off), and c representes low dose group, dose groups during d representes, e representes high dose group.
Fig. 4 is that microscopically amplifies 10 times ileum pathological section figure.
Wherein: a representes normal group, b representation model group (D district representation model group mouse ileum fine hair ruptures, comes off and a large amount of inflammatory cell infiltrations is arranged), and c representes low dose group, dose groups during d representes, e representes high dose group.
Fig. 5 is that microscopically amplifies 10 times colon pathology slice map.
Wherein: a representes normal group, b representation model group (a large amount of inflammatory cell infiltrations of E district representation model group mouse colonic lamina propria), and c representes low dose group, dose groups during d representes, e representes high dose group.
The specific embodiment:
Embodiment 1
Get by traditional processing technology production and through the old Fu brick tea 288kg of storage in 8 years and break into pieces, mix, be soaked in the 2000l distilled water with 10kg sweet wormwood and 2kg dried peppermint leaf; Heat while stirring, to temperature be 85 ℃, kept 20 minutes; Leave standstill; Be cooled to room temperature, filter, gained tea juice is compound Fu brick tea water extract.
Embodiment 2
Get by traditional processing technology production and through the old Fu brick tea 288kg of storage in 2 years and break into pieces, mix, be soaked in the 3000l distilled water with 10kg sweet wormwood and 2kg dried peppermint leaf; Heat while stirring, to temperature be 100 ℃, kept 10 minutes; Leave standstill; Be cooled to room temperature, filter, gained tea juice is compound Fu brick tea water extract.
Embodiment 3
Get by traditional processing technology production and through the old Fu brick tea 288kg of storage in 10 years and break into pieces, mix, be soaked in the 800l distilled water with 10kg sweet wormwood and 2kg dried peppermint leaf; Heat while stirring, to temperature be 70 ℃, kept 30 minutes; Leave standstill; Be cooled to room temperature, filter, gained tea juice is compound Fu brick tea water extract.
Claims (4)
1. compound Fu brick tea water extract, it is characterized in that: this water extract is the ratio that adds 10g sweet wormwood and 2g dried peppermint leaf in every 288g Fu brick tea, gets the Fu brick tea and breaks into pieces; Mix with sweet wormwood and dried peppermint leaf, add the ratio of 8-30ml distilled water again in every 3g Fu brick tea, sweet wormwood and dried peppermint leaf, mixed Fu brick tea, sweet wormwood and dried peppermint leaf are soaked in the distilled water; Heat while stirring again, to temperature be 70-100 ℃, kept 10-30 minute; Leave standstill; Be cooled to room temperature, filter, gained tea juice is compound Fu brick tea water extract.
2. compound Fu brick tea water extract as claimed in claim 1 is characterized in that: said Fu brick tea is meant according to traditional processing technology production and the old old tea stored through 2-10.
3. like the effect of the described compound Fu brick tea water extract of above-mentioned each claim in preparation treatment bacterial diarrhea medicine.
4. application as claimed in claim 3 is characterized in that: said bacterial diarrhea is meant the animal diarrhoea that is caused by Escherichia coli O 157, salmonella, staphylococcus aureus or the inferior Salmonella of enteritis Yale.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100690046A CN102599336B (en) | 2012-03-15 | 2012-03-15 | Composite aqueous extract of Fu brick tea and application thereof |
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| CN2012100690046A CN102599336B (en) | 2012-03-15 | 2012-03-15 | Composite aqueous extract of Fu brick tea and application thereof |
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| CN102599336A true CN102599336A (en) | 2012-07-25 |
| CN102599336B CN102599336B (en) | 2013-03-13 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093264A (en) * | 1993-04-06 | 1994-10-12 | 李维永 | Control swine fever medication and preparation method thereof |
| CN101708275A (en) * | 2009-11-27 | 2010-05-19 | 北京市卫生局临床药学研究所 | Chinese medicament for treating flu caused by influenza A(H1N1) and the like |
-
2012
- 2012-03-15 CN CN2012100690046A patent/CN102599336B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093264A (en) * | 1993-04-06 | 1994-10-12 | 李维永 | Control swine fever medication and preparation method thereof |
| CN101708275A (en) * | 2009-11-27 | 2010-05-19 | 北京市卫生局临床药学研究所 | Chinese medicament for treating flu caused by influenza A(H1N1) and the like |
Non-Patent Citations (3)
| Title |
|---|
| 余智勇: "茯砖茶抗腹泻作用研究", 《中国优秀硕士学位论文全文数据库(电子期刊）医药卫生科技辑》, no. 3, 31 December 2011 (2011-12-31) * |
| 刘平等: "茯砖茶水提物对大肠杆菌感染小鼠的免疫调节作用", 《湖南农业大学学报(自然科学版)》, vol. 37, no. 5, 31 October 2011 (2011-10-31) * |
| 无: "痢疾", 《医学文选》, no. 6, 31 December 1997 (1997-12-31) * |
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