CN102586182A - 人源干细胞分泌生物活性因子与裂解液的制备与应用 - Google Patents
人源干细胞分泌生物活性因子与裂解液的制备与应用 Download PDFInfo
- Publication number
- CN102586182A CN102586182A CN2010105926605A CN201010592660A CN102586182A CN 102586182 A CN102586182 A CN 102586182A CN 2010105926605 A CN2010105926605 A CN 2010105926605A CN 201010592660 A CN201010592660 A CN 201010592660A CN 102586182 A CN102586182 A CN 102586182A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- biologically active
- cell
- stem
- active factors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000000975 bioactive effect Effects 0.000 title abstract 6
- 230000009089 cytolysis Effects 0.000 title abstract 2
- 210000000130 stem cell Anatomy 0.000 claims abstract description 166
- 210000004027 cell Anatomy 0.000 claims abstract description 40
- 210000003954 umbilical cord Anatomy 0.000 claims abstract description 27
- 230000003321 amplification Effects 0.000 claims abstract description 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 20
- 210000005059 placental tissue Anatomy 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 241001465754 Metazoa Species 0.000 claims abstract description 9
- 230000003796 beauty Effects 0.000 claims abstract description 8
- 230000036541 health Effects 0.000 claims abstract description 8
- 230000008439 repair process Effects 0.000 claims abstract description 5
- 229940088623 biologically active substance Drugs 0.000 claims description 28
- 230000004069 differentiation Effects 0.000 claims description 19
- 230000028327 secretion Effects 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 11
- 239000013592 cell lysate Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 238000000108 ultra-filtration Methods 0.000 claims description 7
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 210000005056 cell body Anatomy 0.000 claims description 4
- 239000006166 lysate Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 230000007170 pathology Effects 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 3
- 230000035790 physiological processes and functions Effects 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 239000012510 hollow fiber Substances 0.000 claims description 2
- 206010020718 hyperplasia Diseases 0.000 claims description 2
- 230000001771 impaired effect Effects 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000010257 thawing Methods 0.000 claims description 2
- 230000006870 function Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000032683 aging Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract 1
- 230000006037 cell lysis Effects 0.000 abstract 1
- 230000019522 cellular metabolic process Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000003902 lesion Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 11
- 230000035755 proliferation Effects 0.000 description 10
- 210000002826 placenta Anatomy 0.000 description 7
- 108010019160 Pancreatin Proteins 0.000 description 6
- 229940055695 pancreatin Drugs 0.000 description 6
- 210000003491 skin Anatomy 0.000 description 6
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 5
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 4
- 102000004889 Interleukin-6 Human genes 0.000 description 4
- 108090001005 Interleukin-6 Proteins 0.000 description 4
- 102100021592 Interleukin-7 Human genes 0.000 description 4
- 108010002586 Interleukin-7 Proteins 0.000 description 4
- 102000036693 Thrombopoietin Human genes 0.000 description 4
- 108010041111 Thrombopoietin Proteins 0.000 description 4
- 102000018594 Tumour necrosis factor Human genes 0.000 description 4
- 108050007852 Tumour necrosis factor Proteins 0.000 description 4
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 4
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 4
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 2
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 2
- 102000014150 Interferons Human genes 0.000 description 2
- 108010050904 Interferons Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 210000001691 amnion Anatomy 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000003915 cell function Effects 0.000 description 2
- 210000001136 chorion Anatomy 0.000 description 2
- 210000001671 embryonic stem cell Anatomy 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 229940079322 interferon Drugs 0.000 description 2
- 229940100601 interleukin-6 Drugs 0.000 description 2
- 229940100994 interleukin-7 Drugs 0.000 description 2
- 230000004719 natural immunity Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 102000005606 Activins Human genes 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000006909 anti-apoptosis Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000002608 insulinlike Effects 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 210000002444 unipotent stem cell Anatomy 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
Claims (12)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105926605A CN102586182A (zh) | 2010-12-17 | 2010-12-17 | 人源干细胞分泌生物活性因子与裂解液的制备与应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105926605A CN102586182A (zh) | 2010-12-17 | 2010-12-17 | 人源干细胞分泌生物活性因子与裂解液的制备与应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN102586182A true CN102586182A (zh) | 2012-07-18 |
Family
ID=46475418
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2010105926605A Pending CN102586182A (zh) | 2010-12-17 | 2010-12-17 | 人源干细胞分泌生物活性因子与裂解液的制备与应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102586182A (zh) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103550117A (zh) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | 可促进皮肤修复与再生的美容护肤品及其制备方法和应用 |
CN103767985A (zh) * | 2012-10-22 | 2014-05-07 | 吉林省霍普金斯药物研究院有限责任公司 | 人源血液或间充质干细胞分泌exosome的制备与应用 |
CN104099294A (zh) * | 2013-04-03 | 2014-10-15 | 北京京蒙高科干细胞技术有限公司 | 基于干细胞分泌因子的培养基及其制备和使用方法 |
CN104224841A (zh) * | 2014-09-13 | 2014-12-24 | 黑龙江天晴干细胞有限公司 | 干细胞制剂的制备方法 |
CN104490931A (zh) * | 2014-12-17 | 2015-04-08 | 江苏大学 | 人脐带间质干细胞分泌的exosome治疗皮肤损伤的应用 |
CN105505851A (zh) * | 2014-10-14 | 2016-04-20 | 讯联生物科技股份有限公司 | 促进真皮***细胞生长的组合物及其医药组合与制备方法 |
CN105878283A (zh) * | 2016-05-31 | 2016-08-24 | 张正亮 | 人源干细胞生物活性物质及其制备方法 |
CN106038598A (zh) * | 2016-05-31 | 2016-10-26 | 张正亮 | 人源干细胞分泌生物活性因子与裂解液的制备方法 |
CN106381284A (zh) * | 2016-09-12 | 2017-02-08 | 博雅干细胞科技有限公司 | 制备干细胞活性因子的方法 |
CN106701670A (zh) * | 2015-08-05 | 2017-05-24 | 朱轶 | 一种增强间充质干细胞分泌生物活性因子能力及培养液中活性因子的提取方法 |
CN106727704A (zh) * | 2016-12-26 | 2017-05-31 | 云南农业大学 | 犬用干细胞分泌因子注射剂 |
TWI612136B (zh) * | 2013-01-09 | 2018-01-21 | Bionet Corp | 含有分泌蛋白質組的組成物及其製備方法與用途 |
CN108578264A (zh) * | 2018-05-24 | 2018-09-28 | 广州准优生物科技有限公司 | 胎盘素及其制备、应用 |
CN108670942A (zh) * | 2018-06-22 | 2018-10-19 | 珠海市雅莎医疗器械有限公司 | 一种基于干细胞技术制备的抗衰祛皱精华液及其制备方法 |
CN109971707A (zh) * | 2019-03-28 | 2019-07-05 | 启迪汉洱康(嘉兴)生物科技有限公司 | 一种细胞胞体来源胞内功能生理活性肽库的构建方法 |
CN110339147A (zh) * | 2018-11-27 | 2019-10-18 | 浙江梵泊细胞工程有限公司 | 一种皮肤外用组合物及其用途 |
CN110368356A (zh) * | 2019-08-08 | 2019-10-25 | 北京京蒙细胞生物科技股份有限公司 | 皮肤损伤修复因子组合物及其制备方法 |
CN110507597A (zh) * | 2019-08-20 | 2019-11-29 | 苏州诺普再生医学有限公司 | 一种组合物及其制备方法、应用 |
CN112315978A (zh) * | 2019-07-19 | 2021-02-05 | 丰泽康生物医药(深圳)有限公司 | 外周血多潜能细胞活性物与富血小板血浆复合物及其制备方法和应用 |
CN114159375A (zh) * | 2021-12-24 | 2022-03-11 | 广州安立美健康生物科技有限公司 | 一种用于护肤品制剂的干细胞破碎液的制备方法及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101270349A (zh) * | 2008-03-20 | 2008-09-24 | 浙江大学 | 胎盘间充质干细胞分离和体外扩增培养方法 |
CN101831403A (zh) * | 2010-05-21 | 2010-09-15 | 协和干细胞基因工程有限公司 | 体外扩增人脐带胎盘间充质干细胞的方法 |
CN102465148A (zh) * | 2010-11-01 | 2012-05-23 | 张正前 | 人源干细胞生物活性物质及其制备与应用 |
-
2010
- 2010-12-17 CN CN2010105926605A patent/CN102586182A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101270349A (zh) * | 2008-03-20 | 2008-09-24 | 浙江大学 | 胎盘间充质干细胞分离和体外扩增培养方法 |
CN101831403A (zh) * | 2010-05-21 | 2010-09-15 | 协和干细胞基因工程有限公司 | 体外扩增人脐带胎盘间充质干细胞的方法 |
CN102465148A (zh) * | 2010-11-01 | 2012-05-23 | 张正前 | 人源干细胞生物活性物质及其制备与应用 |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103767985A (zh) * | 2012-10-22 | 2014-05-07 | 吉林省霍普金斯药物研究院有限责任公司 | 人源血液或间充质干细胞分泌exosome的制备与应用 |
TWI612136B (zh) * | 2013-01-09 | 2018-01-21 | Bionet Corp | 含有分泌蛋白質組的組成物及其製備方法與用途 |
CN104099294A (zh) * | 2013-04-03 | 2014-10-15 | 北京京蒙高科干细胞技术有限公司 | 基于干细胞分泌因子的培养基及其制备和使用方法 |
CN103550117A (zh) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | 可促进皮肤修复与再生的美容护肤品及其制备方法和应用 |
CN104224841A (zh) * | 2014-09-13 | 2014-12-24 | 黑龙江天晴干细胞有限公司 | 干细胞制剂的制备方法 |
CN105505851A (zh) * | 2014-10-14 | 2016-04-20 | 讯联生物科技股份有限公司 | 促进真皮***细胞生长的组合物及其医药组合与制备方法 |
CN104490931A (zh) * | 2014-12-17 | 2015-04-08 | 江苏大学 | 人脐带间质干细胞分泌的exosome治疗皮肤损伤的应用 |
CN104490931B (zh) * | 2014-12-17 | 2018-08-21 | 江苏大学 | 人脐带间质干细胞分泌的exosome治疗皮肤损伤的应用 |
CN106701670A (zh) * | 2015-08-05 | 2017-05-24 | 朱轶 | 一种增强间充质干细胞分泌生物活性因子能力及培养液中活性因子的提取方法 |
CN106038598A (zh) * | 2016-05-31 | 2016-10-26 | 张正亮 | 人源干细胞分泌生物活性因子与裂解液的制备方法 |
CN105878283A (zh) * | 2016-05-31 | 2016-08-24 | 张正亮 | 人源干细胞生物活性物质及其制备方法 |
CN106381284B (zh) * | 2016-09-12 | 2020-06-23 | 博雅干细胞科技有限公司 | 制备干细胞活性因子的方法 |
CN106381284A (zh) * | 2016-09-12 | 2017-02-08 | 博雅干细胞科技有限公司 | 制备干细胞活性因子的方法 |
CN106727704A (zh) * | 2016-12-26 | 2017-05-31 | 云南农业大学 | 犬用干细胞分泌因子注射剂 |
CN108578264A (zh) * | 2018-05-24 | 2018-09-28 | 广州准优生物科技有限公司 | 胎盘素及其制备、应用 |
CN108670942A (zh) * | 2018-06-22 | 2018-10-19 | 珠海市雅莎医疗器械有限公司 | 一种基于干细胞技术制备的抗衰祛皱精华液及其制备方法 |
CN110339147A (zh) * | 2018-11-27 | 2019-10-18 | 浙江梵泊细胞工程有限公司 | 一种皮肤外用组合物及其用途 |
CN109971707A (zh) * | 2019-03-28 | 2019-07-05 | 启迪汉洱康(嘉兴)生物科技有限公司 | 一种细胞胞体来源胞内功能生理活性肽库的构建方法 |
CN112315978A (zh) * | 2019-07-19 | 2021-02-05 | 丰泽康生物医药(深圳)有限公司 | 外周血多潜能细胞活性物与富血小板血浆复合物及其制备方法和应用 |
CN110368356A (zh) * | 2019-08-08 | 2019-10-25 | 北京京蒙细胞生物科技股份有限公司 | 皮肤损伤修复因子组合物及其制备方法 |
CN110507597A (zh) * | 2019-08-20 | 2019-11-29 | 苏州诺普再生医学有限公司 | 一种组合物及其制备方法、应用 |
CN114159375A (zh) * | 2021-12-24 | 2022-03-11 | 广州安立美健康生物科技有限公司 | 一种用于护肤品制剂的干细胞破碎液的制备方法及应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102586182A (zh) | 人源干细胞分泌生物活性因子与裂解液的制备与应用 | |
CN102465148A (zh) | 人源干细胞生物活性物质及其制备与应用 | |
CN106109496B (zh) | 人脐带间充质干细胞提取物冻干粉及制备方法 | |
CN102552099B (zh) | 一种用于皮肤美容修复的复合生物制剂及制备方法 | |
CN105154395B (zh) | 一种增强MSCs免疫调节功能的临床级别细胞制备方法 | |
CN108309822A (zh) | 一种人脐带间充质干细胞旁分泌因子冻干粉的制备方法 | |
CN108721200A (zh) | 一种人间充质干细胞来源的外泌体美容制剂的制备方法及应用 | |
CN106038598A (zh) | 人源干细胞分泌生物活性因子与裂解液的制备方法 | |
CN106659741B (zh) | 小尺寸干细胞的毛发生长促进功能及其用途 | |
CN104087551B (zh) | 一种体外分离培养人的表皮细胞的方法 | |
CN101461772A (zh) | 用于美容护肤的干细胞分泌因子的制备方法 | |
CN103352026A (zh) | 人脐带血富血小板裂解液培养自体脐带间充质干细胞方法 | |
CN103191154B (zh) | 充质干细胞及其提取方法在制备银屑病的药物中的应用 | |
CN105062959A (zh) | 一种人羊膜间充质干细胞的分离培养方法 | |
CN104099294A (zh) | 基于干细胞分泌因子的培养基及其制备和使用方法 | |
KR20140040696A (ko) | 간엽줄기세포 및 면역조절 t 세포를 유효성분으로 포함하는 면역질환의 예방 또는 치료용 세포치료제 조성물 | |
CN105861432A (zh) | 人源脐带血造血干细胞体外高效扩增方法 | |
CN103898049A (zh) | 一种活细胞精华液产品及其制备方法和应用 | |
CN103494865A (zh) | 人源干细胞与绞股蓝生物活性物质组合物及其制备方法 | |
CN102965337A (zh) | 分离提取人皮下脂肪间充质干细胞的方法和提取专用培养基 | |
CN106701670A (zh) | 一种增强间充质干细胞分泌生物活性因子能力及培养液中活性因子的提取方法 | |
CN109985064A (zh) | 间充质干细胞分泌提取物的用途、间充质干细胞分泌提取物及其制备方法 | |
CN102949418A (zh) | 人源干细胞与灵芝孢子生物活性物质组合物及其制备方法 | |
CN111568851A (zh) | 一种利用围产期msc生产活性因子的方法及美容制剂 | |
CN106191127A (zh) | 干细胞生物活性组合物及其制备方法与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
DD01 | Delivery of document by public notice |
Addressee: Zhang Zhengqian Document name: Notification of Passing Preliminary Examination of the Application for Invention |
|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: HEFEI HUI EN BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHANG ZHENGQIAN Effective date: 20140806 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Kaibin Inventor after: Zhang Zhengqian Inventor before: Zhang Zhengqian |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG ZHENGQIAN TO: ZHANG KAIBIN ZHANG ZHENGQIAN Free format text: CORRECT: ADDRESS; FROM: 230603 HEFEI, ANHUI PROVINCE TO: 230031 HEFEI, ANHUI PROVINCE |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20140806 Address after: 401, room 7, 230031 East diving East Road, hi tech Zone, Anhui, Hefei Applicant after: Hefei Hui'en Biological Technology Co., Ltd. Address before: 230603, Room 301, new building, dormitory of Shushan management office, Jinzhai road and Danxia Road, Hefei economic and Technological Development Zone, Anhui, China Applicant before: Zhang Zhengqian |
|
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120718 |