CN102565385A - Rapid detection instrument for epidemic antigen and antibody of living animal - Google Patents
Rapid detection instrument for epidemic antigen and antibody of living animal Download PDFInfo
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- CN102565385A CN102565385A CN2011104448085A CN201110444808A CN102565385A CN 102565385 A CN102565385 A CN 102565385A CN 2011104448085 A CN2011104448085 A CN 2011104448085A CN 201110444808 A CN201110444808 A CN 201110444808A CN 102565385 A CN102565385 A CN 102565385A
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Abstract
A rapid detection instrument for epidemic antigen antibody of a living animal is disclosed, which comprises a plastic protection casing, an animal epidemic antigen or antibody immunochromatography reagent strip and a sample collector which are arranged in the plastic protection casing, wherein the animal epidemic antigen or antibody immunochromatography reagent strip comprises a sample applying zone, a reaction zone and a water absorbing zone which are arranged in sequence; the sample collector is in a capillary micro-pore substrate structure and comprises a handle part, a board part and a combining part which connects the handle part with the board part, and the sample collector is used for collecting a living animal liquid sample and automatically separating and conveying the animal epidemic antigen or antibody in the liquid sample; the plastic protection casing comprises a bottom plate and a face plate arranged in conjunction with the bottom plate, the face plate is provided with an observation window used for interpreting a detection result, and the observation window and the reaction zone of the animal epidemic antigen or antibody immunochromatography reagent strip are correspondingly arranged; and the handle part of the sample collector is partially overlapped and placed above the sample applying zone of the animal epidemic antigen or antibody immunochromatography reagent strip, the board part extends out of the plastic protection casing, and the living animal liquid sample is the natural short cavity orifice liquid sample or wound liquid sample of the living animal.
Description
Technical field
The present invention is about a kind of fast detecting appearance, relates in particular to a kind of living animal eqpidemic disease antigen-antibody fast detecting appearance that can effectively detect animal epidemic antigen in living animal nature short cavity road or the surface of a wound liquid sample or antibody.
Background technology
China is large agricultural country, also is herding big country.Culturing with the domestic animal pig is example, and national domestic animal pig livestock on hand was about 4.7 hundred million in 2009, delivered 6.45 hundred million for sale, and 4,889 ten thousand tons of pork output account for 50% of global total production, rank the first in the world.But China is not herding power.Every sow annual childbirth tire number, every aspects such as number pigs weaned, piglet survival rate and the pork pig rate of animals delivered to the slaughter-house being provided sow year, making the country prosperous with world's herding---the U.S. compares, and still has greatest differences.Its reason is that the intensive culture degree is not high, and scale is not enough, and peasant household raises scattered or miniaturization plant proportion height, causes eqpidemic disease multiple, and yield-power is low, and interpolation forbidden drug and abuse of antibiotics phenomenon are serious, and environmental pollution is serious.
Because what Chinese aquaculture proportion was bigger is that peasant household raises scattered or small-sized plant cultures, scale is disperseed, and animal epidemic prevents and treats level and the production management ability falls behind, and when pestilence takes place, is difficult to effective control.Especially in vast rural area; The control of animal epidemic is relied on one-level animal and veterinary station, small towns and is accredited the village animal doctor outward; These practitioners often do not accept regular animal doctor's basic education and skills training; Most animal doctors for the cultivation of " passing on experience (being that rural animal doctor master worker trains an apprentice or carries on parent's cause) " pattern lack the most basic animal doctor's diagnostic equipment and utensil simultaneously, are difficult to tackle the animal epidemic prevention and control and medical treatment of increased popularity, sternness day by day.In addition, many also double plants that offer of regional rural animal doctor propagate into self plant in order to reduce animal epidemic, the animal epidemic problem of other peasant households of rural animal doctor's intractable.
Though; Animal epidemic reagent strip for immunochromatographydetection detection product simple to operate appears on the market in a large number; But the overwhelming majority needs sample of blood, drawn; Even portioned product also need carry out necessary processing to blood sample, and this is as far as lacking the vast animal doctor of basic unit and the peasant household of animal doctor's basic education and skills training, very difficulty be not easy to realize.
Lot of domestic and foreign scientific research result confirms; For the most eqpidemic diseases of living animal; Its target detection thing that can be used for Clinical detection is such as living animal oral cavity juice or urethral orifice debris or natural short cavity such as ight soil or milk road (the Natural Short Cavity; NSC) be consistent with blood the opportunity that occurs in liquid sample and surface of a wound blood or the liquid samples such as purulent secretion liquid or non-purulent secretion liquid, only aspect content, there are differences.Extensively exist in animal mouth cavity liquids such as dog, cat and pig like hydrophobin, rabies virus antibodies IgG and IgM, toxoplasma antibody etc. also distribute in the animal mouth cavity liquid, and toxoplasma antigen also is present in animal oral cavity and the ight soil.So that develops interpretation testing result easy to use, simple to operate, easy includes but not limited to that using liquid samples such as oral cavity juice or urethral orifice debris or natural short cavity such as ight soil or milk road (NSC) liquid sample and surface of a wound blood or purulent secretion liquid or non-purulent secretion liquid detects living animal eqpidemic disease antigen or antibody fast detecting product; China is developed the herding industry present stage be significant, and market reward also quite there is prospect.
Summary of the invention
The object of the present invention is to provide a kind of living animal eqpidemic disease antigen-antibody fast detecting appearance; Effective qualitative detection living animal nature short cavity road (the Natural Short Cavity; NSC) various animal epidemic antigens or antibody in liquid sample (like oral cavity juice, urethral orifice debris, ight soil, milk) or the surface of a wound liquid sample (like blood, purulent secretion liquid or non-purulent secretion liquid); Be used to diagnose or whether examination living animal body infects certain infectious disease or analyze living animal and whether possess the resistibility to certain eqpidemic disease, for the animal doctor or raise main control animal epidemic foundation is provided.
For realizing above-mentioned purpose; The present invention provides a kind of living animal eqpidemic disease antigen-antibody fast detecting appearance; Comprise: the plastics containment vessel, place animal epidemic antigen or antibody immune chromatography reagent strip in the plastics containment vessel, and sample collecting device, this animal epidemic antigen or antibody immune chromatography reagent strip comprise sample application zone, reaction zone and the suction zones that sets gradually; Sample collecting device has capillary micropore matrix structure, comprises shank, board and connects the joint portion of shank and board, and this sample collecting device is used to gather the living animal liquid sample, and separates voluntarily and carry animal epidemic antigen or the antibody in the liquid sample; The panel that the plastics containment vessel comprises base plate and is equipped with base plate, panel is provided with the watch window that is used for the interpretation testing result, the corresponding setting of reaction zone of watch window and animal epidemic antigen or antibody immune chromatography reagent strip; The sample application zone top that the shank of sample collecting device is overlapped and is positioned over animal epidemic antigen or antibody immune chromatography reagent strip; Board stretches out outside the plastics containment vessel, and said living animal liquid sample is living animal nature short cavity road liquid sample or surface of a wound liquid sample.
Said natural short cavity road liquid sample comprises oral cavity juice, urethral orifice debris, ight soil and milk, and surface of a wound liquid sample comprises blood, purulent secretion liquid and non-purulent secretion liquid.
Also comprise developping agent; Make an addition on the sample collecting device; With the pH value of regulating the living animal liquid sample and directivity and the flow velocity that guarantees the transportation of living animal liquid sample, make animal epidemic antigen or antibody immune chromatography reagent strip obtain enough living animal liquid samples; This developping agent splendid attire is in the transparent rigid plastic bottle of sealing.
Said developping agent is that a kind of pH value is the compound damping fluid of 6.5-8.5, and is formulated in proportion by potassium dihydrogen phosphate, trishydroxymethylaminomethane, sodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20, thimerosal, lauryl sodium sulfate, ethylenediamine tetraacetic acid, deionized water.
Said plastics containment vessel one end is made as the holding area, and the base plate of plastics containment vessel is provided with several air holes in an end of holding area, and its inboard is made as the sample collecting device assembly section away from an end of holding area, and the sample collecting device stationary installation is set on it; The middle part is made as animal epidemic antigen or antibody immune chromatography reagent strip assembly section, and it is provided with the stationary installation that supplies to place animal epidemic antigen or antibody immune chromatography reagent strip.
Said watch window top is bonded with an eyeglass, and the eyeglass marked has " T ", " C " to indicate line, respectively with the detection line and the corresponding setting of nature controlling line of animal epidemic antigen or antibody immune chromatography reagent strip; Eyeglass is processed by transparent rigid plastic.
The base plate of said plastics containment vessel and panel inboard are provided with coupling arrangement, are used for base plate is fixedly connected with panel, and fixed connection method adopts the method for withholding or adopts the ultrasonic plastic welder welding to be fixedly connected.
Said animal epidemic antigen or antibody immune chromatography reagent strip adopt colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology, and this animal epidemic antigen or antibody immune chromatography reagent strip comprise animal epidemic antigen immune chromatography reagent strip, reach animal epidemic antibody immune chromatography reagent strip.
Said sample collecting device is processed by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure; It is 8%～80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre is cotton fiber, filter paper, sponge or cloth; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, nylon and polyethersulfone and other plastics, glass or resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1; The profile of sample collecting device is table tennis plate or slurry shape, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than sample collecting device total surface area 50%; 20～500 microns of the average pore sizes of capillary micropore matrix, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.21～0.86 gram/cubic centimetre.
Said sample collecting device is processed by one or more hydrophobic materials, and this hydrophobic material forms capillary micropore matrix structure; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass or resin; The sample collecting device profile is bar-shaped or tabular, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than 50% of sample collecting device total surface area; 20～500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49～0.92 gram/cubic centimetre.
Also comprise bridge, it is a strip, and bridge one end is made as handheld terminal, and an other end is made as installation end so that said living animal eqpidemic disease antigen-antibody fast detecting appearance to be installed; This installation end is provided with notch, the corresponding setting of plastics containment vessel holding area periphery of this notch and living animal eqpidemic disease antigen-antibody fast detecting appearance.
Beneficial effect of the present invention: the present invention is qualitative detection living animal nature short cavity road (the Natural Short Cavity effectively; NSC) various animal epidemic antigens or antibody in liquid sample (like oral cavity juice, urethral orifice debris, ight soil, milk) or the surface of a wound liquid sample (like blood, purulence or non-purulent secretion liquid); Be used to diagnose or whether examination living animal body infects certain infectious disease or analyze living animal and whether possess the resistibility to certain eqpidemic disease, for the animal doctor or raise main control animal epidemic foundation is provided.And:
1), protection employment security: reduce the time that directly contacts with animal, reduce the chance that enrages animal, cooperate special-purpose bridge to protect tester's safety better.
2), increase work efficiency: simple to operate, convenient to use, need not professional equipment, do not need professional skill yet, effectively improve tester's work efficiency.
Description of drawings
Below in conjunction with accompanying drawing, describe in detail through specific embodiments of the invention, will make technical scheme of the present invention and other beneficial effects obvious.
In the accompanying drawing,
Fig. 1 is the combination synoptic diagram of living animal eqpidemic disease antigen-antibody fast detecting appearance of the present invention;
Fig. 2 is the decomposing schematic representation of Fig. 1;
Fig. 3 is animal epidemic antigen or the synoptic diagram of antibody immune chromatography reagent strip among Fig. 2;
Fig. 4 is the synoptic diagram of the sample collecting device among Fig. 2;
Fig. 5 is the assembling synoptic diagram of each several part;
Fig. 6 is the structural representation of bridge one embodiment among the present invention.
Embodiment
Shown in Fig. 1-5, living animal eqpidemic disease antigen-antibody fast detecting appearance of the present invention comprises: plastics containment vessel 2, place animal epidemic antigen or antibody immune chromatography reagent strip 4 in the plastics containment vessel 2, and sample collecting device 6.
Wherein, animal epidemic antigen or antibody immune chromatography reagent strip 4 comprise sample application zone 42, reaction zone 44 and the suction zones 46 that sets gradually, and reaction zone 44 is connected between sample application zone 42 and the suction zones 46, and reaction zone 44 is provided with detection line 45 and nature controlling line 47; Plastics containment vessel 2 comprises base plate 22; The panel 24 that is equipped with base plate 22; Base plate 22 is fixedly connected through its inboard coupling arrangement 29 that is provided with panel 24, and fixing means can adopt the method for withholding, and also can adopt the ultrasonic plastic welder welding to be fixedly connected.Plastics containment vessel 2 one ends are made as holding area 23; Base plate 22 is provided with several air holes 21 in holding area 23; Base plate 22 inboards are made as sample collecting device assembly section 25 away from an end of holding area 23, and sample collecting device stationary installation 252 is set on it, are used to settle sample collecting device 6; The middle part is made as animal epidemic antigen or antibody immune chromatography reagent strip assembly section 26, and reagent strip stationary installation 262 is set respectively on it, in order to place animal epidemic antigen or antibody immune chromatography reagent strip 4.Panel 24 is provided with the watch window 27 that is used for the interpretation testing result, watch window 27 and reaction zone 25 corresponding settings; Above watch window 27, be bonded with an eyeglass 28, eyeglass 28 marked have " T ", " C " to indicate line, respectively with detection line of animal epidemic antigen or antibody immune chromatography reagent strip 45 and nature controlling line 47 corresponding settings; Said eyeglass 28 is processed by transparent rigid plastic.
Said sample collecting device 6 has capillary micropore matrix structure; The joint portion 66 that comprises shank 62, board 64 and connection shank 62 and board 64; This sample collecting device is used to gather the living animal liquid sample, and separates voluntarily and carry animal epidemic antigen or the antibody in the liquid sample; Sample application zone 42 tops that the shank 62 of sample collecting device 6 is overlapped and is positioned over animal epidemic antigen or antibody immune chromatography reagent strip 4, board 64 stretches out outside the plastics containment vessel 2.Said living animal liquid sample is living animal nature short cavity road liquid sample or surface of a wound liquid sample; Nature short cavity road liquid sample comprises oral cavity juice, urethral orifice debris, ight soil and milk, and surface of a wound liquid sample comprises blood, purulent secretion liquid and non-purulent secretion liquid.Living animal liquid sample and wherein animal epidemic antigen or antibody are absorbed, separate, are delivered to the sample application zone 42 of animal epidemic antigen or antibody immune chromatography reagent strip 4 by sample collecting device 6; Utilize chromatographic theory to be delivered to reaction zone 44 again; And a series of immunological responses take place at reaction zone 44, demonstrate the directly result of interpretation (like red stripes) of the watch window 27 of naked eyes through panel.
Said animal epidemic antigen or antibody immune chromatography reagent strip 4 adopt colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology, so are not limited thereto.This animal epidemic antigen or antibody immune chromatography reagent strip 4 comprise animal epidemic antigen immune chromatography reagent strip, reach animal epidemic antibody immune chromatography reagent strip; Detect antigen or antibody situation in living animal nature short cavity road or the surface of a wound liquid through this animal epidemic antigen or antibody immune chromatography reagent strip; Whether infect certain infectious disease or analyze living animal whether possess resistibility with diagnosis or examination living animal body, foundation is provided for the animal doctor perhaps raises main control animal epidemic to certain eqpidemic disease.
Said sample collecting device 6 is processed by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure, have the effect that separates voluntarily and carry the target detection thing in the liquid sample.It is 8%～80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre can be cotton fiber, filter paper, sponge, cloth; Hydrophobic material can be tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, also can be glass, resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1.These sample collecting device 6 profiles can be soldier's pang plate or oar shape, its parameter: total surface area is not less than 700 square millimeters, cumulative volume and is not less than 650 cubic millimeters, the surface area of sample collecting device board and is not less than 50% of sample collecting device total surface area; 20～500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of this sample collecting device is 0.21～0.86 gram/cubic centimetre.In this sample collecting device, preferred parameter be hydrophilic fibre to account for sample collecting device general assembly (TW) ratio range be 10%～58%, hydrophilic fibre is preferably cotton fiber, hydrophobic material is preferably polyester; Cotton fiber is preferably 1: 9 to 3: 2 with weight polyester than scope; The average pore size of capillary micropore matrix structure is preferably 20～200 microns, and the total void rate is preferably 40～45%, and the density of sample collecting device 6 is preferably 0.48～0.52 gram/cubic centimetre.
As another kind of selectivity embodiment; Said sample collecting device 6 is processed by hydrophobic material; Can be processed by one or more hydrophobic materials, this hydrophobic material forms capillary micropore matrix structure, has the effect that separates voluntarily and carry the target detection thing in the liquid sample.Hydrophobic material can be tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass, resin etc.; This sample collecting device profile can be bar-shaped or tabular, its parameter: total surface area is not less than 700 square millimeters, cumulative volume and is not less than 650 cubic millimeters, the surface area of sample collecting device board and is not less than 50% of sample collecting device total surface area; 20～500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49～0.92 gram/cubic centimetre.In this sample collecting device, preferred parameter is the hydrophobic material preferred polyester; The average pore size of capillary micropore matrix structure is preferably 20～200 microns, total void rate and is preferably 35～55%, and the density of sample collecting device is preferably 0.58～0.72 gram/cubic centimetre.This hydrophobic material also can pass through hydrophilic treated and process sample collecting device again, or the sample collecting device that will be processed by hydrophobic material is through hydrophilic treated, with the ability that improves the sample collecting device collection and carry liquid sample.
Above-mentioned sample collecting device 6 is controlled at below 500 cubic millimeters through the amount of its capillary micropore matrix structure saturated absorption liquid sample; Time with immersion way saturated absorption liquid sample was controlled in 5 minutes; In preferred 1 minute, behind the sample collecting device saturated absorption liquid sample, its inner structure requires not take place significant change; Especially after passing through dried, tangible change does not take place in its rate of water absorption.Under the prerequisite that does not have extraneous mechanical compress or filtration, be adsorbed in liquid sample in the capillary micropore matrix structure runs to shank 62 edges from board 64 edges time to be controlled in 10 minutes, preferably in 2 minutes.
Also comprise developping agent (not shown), be used that can make an addition on the sample collecting device 6, this developping agent is placed in the transparent rigid plastic bottle of sealing with this living animal eqpidemic disease antigen-antibody fast detecting appearance.Described developping agent is different from damping fluids such as laboratory PBS commonly used or Tris; It is the composite buffer system of a kind of pH value between 6.5～8.5; It act as the pH value of regulating living animal oral cavity juice, urethral orifice debris, ight soil, milk or surface of a wound liquid sample; Make it to meet the potential of hydrogen needs of animal epidemic antigen or antibody immune chromatography reagent strip 4 detection animal epidemics detection marks; Guarantee directivity and flow velocity that living animal oral cavity juice, urethral orifice debris, ight soil, milk or surface of a wound liquid sample are carried; Fill up the space that after animal epidemic antigen or the conveying of antibody immune chromatography reagent strip 4 directions, on sample collecting device, stays because of living animal oral cavity juice, urethral orifice debris, ight soil, milk or surface of a wound liquid sample; And it is flowed to animal epidemic antigen or antibody immune chromatography reagent strip 4 directions with developping agent; Thereby make animal epidemic antigen or antibody immune chromatography reagent strip 4 obtain enough living animal oral cavity juice, urethral orifice debris, ight soil, milk or surface of a wound liquid sample; And animal epidemic antigen in promotion living animal oral cavity juice, urethral orifice debris, ight soil, milk or the surface of a wound liquid sample or antibody separate voluntarily and carry; Effectively improve animal epidemic antigen or the front end concentration of antibody in solvent (comprising liquid sample and developping agent), satisfy the needs of fast detecting living animal eqpidemic disease antigen or antibody.The component of every 1L developping agent is: potassium dihydrogen phosphate 0.2g, trishydroxymethylaminomethane 6.06g, sodium hydrogen phosphate 1.15g, sodium chloride 8.0g, potassium chloride 0.2g, Tween-20 0.5ml, thimerosal 0.1g, lauryl sodium sulfate 1.25g, ethylenediamine tetraacetic acid 1ml; Add deionized water dissolving to 1L solution; Rigid plastic bottle splendid attire with transparent sealing; Every bottle of 0.8～3.0ml, room temperature preservation.
In the present embodiment, the base plate 22 of plastics containment vessel 2 is a connecting line with the panel 24 inboard coupling arrangements 29 that are provided with, and adopts the ultrasonic plastic welder welding to be fixedly connected.During installation; In the ultrasonic plastic welder mould, lay the base plate 22 of plastics containment vessel 2; Assemble animal epidemic antigen or antibody immune chromatography reagent strip 4 and sample collecting device 6 successively in base plate 22 inboards; Make the shank 62 of sample collecting device 6 and the sample application zone 42 overlapping 1-3 millimeters of animal epidemic antigen or antibody immune chromatography reagent strip 4; The panel 24 of plastics containment vessel 2 is installed then; Eyeglass 28 is installed in testing result watch window 27 positions at panel 24 middle parts, with ultrasonic plastic welder the base plate 22 of plastics containment vessel 2 is also fixed welding with the panel 24 of panel 24 and plastics containment vessel 2 with the sealing wire fusion of eyeglass 28, thereby forms the living animal eqpidemic disease antigen-antibody fast detecting appearance of an integral body.
During detection; Utilize the board 64 of sample collecting device 6 to absorb the living animal liquid sample; And be transported to the sample application zone 42 of animal epidemic antigen or antibody immune chromatography reagent strip 4 through the shank 62 of sample collecting device 6; Immunochromatography principle through this reagent strip makes animal epidemic antigen in the sample or antibody be transported to the reaction zone 44 of this reagent strip, and animal epidemic antigen in the living animal liquid sample or antibody are carried out fast detecting, and a series of biology, chemical reaction take place this reaction zone 44; And demonstrate colored band at reaction zone, can read testing result in view of the above.
With reference to shown in Figure 6, the present invention also comprises bridge 8, and it is a strip, as can be the assembly etc. of right cylinder, prism or right cylinder and prism, and bridge 8 can be processed by materials such as plastics, metal or bamboo wood.This bridge 8 is used for being used with said living animal eqpidemic disease antigen-antibody fast detecting appearance, and bridge 8 one ends are made as handheld terminal 82, and an other end is made as installation end 84 so that said living animal eqpidemic disease antigen-antibody fast detecting appearance to be installed.This installation end 84 is provided with notch 842, the corresponding setting of plastics containment vessel 2 holding areas 23 peripheries of this notch 842 and living animal eqpidemic disease antigen-antibody fast detecting appearance, and said fast detecting appearance can cooperate and is mounted thereon.Said bridge 8 length are no less than 30 centimetres, so that be formed with certain safe distance between tester and the living animal.In addition; Also can be provided with locking device (not shown) on the bridge 8 to lock this fast detecting appearance; Locking device can be the universal button be located on bridge 8 installation ends 84, knob, rubber band bundle, bandage, sticker, anchor clamps etc., all can implement as long as can lock this fast detecting appearance.During use; The tester can install this fast detecting appearance on the bridge 8; Utilize the fast detecting appearance that living animal is sampled through rod grip frame 8 handheld terminals 82, can make tester and living animal keep certain safe distance like this, reduce or avoid the tester to be taken place by incidents such as living animal injuries.
In sum; Living animal eqpidemic disease antigen-antibody fast detecting appearance of the present invention combines sample collecting device and animal epidemic antigen or antibody immune chromatography reagent strip; Effectively various eqpidemic disease antigens or antibody in qualitative detection living animal nature short cavity road liquid sample (like oral cavity juice, urethral orifice debris, ight soil, milk) or the surface of a wound liquid sample (like blood, purulence or non-purulent secretion liquid); Be used to diagnose or whether examination living animal body infects certain infectious disease or analyze living animal and whether possess the resistibility to certain eqpidemic disease, for the animal doctor or raise main control animal epidemic foundation is provided; And can protect employment security, increase work efficiency.
The above for the person of ordinary skill of the art, can make other various corresponding changes and distortion according to technical scheme of the present invention and technical conceive, and all these changes and distortion all should belong to the scope that the present invention protects.
Claims (10)
1. living animal eqpidemic disease antigen-antibody fast detecting appearance; It is characterized in that; Comprise: the plastics containment vessel, place animal epidemic antigen or antibody immune chromatography reagent strip in the plastics containment vessel, and sample collecting device, this animal epidemic antigen or antibody immune chromatography reagent strip comprise sample application zone, reaction zone and the suction zones that sets gradually; Sample collecting device has capillary micropore matrix structure, comprises shank, board and connects the joint portion of shank and board, and this sample collecting device is used to gather the living animal liquid sample, and separates voluntarily and carry animal epidemic antigen or the antibody in the liquid sample; The panel that the plastics containment vessel comprises base plate and is equipped with base plate, panel is provided with the watch window that is used for the interpretation testing result, the corresponding setting of reaction zone of watch window and animal epidemic antigen or antibody immune chromatography reagent strip; The sample application zone top that the shank of sample collecting device is overlapped and is positioned over animal epidemic antigen or antibody immune chromatography reagent strip; Board stretches out outside the plastics containment vessel, and said living animal liquid sample is living animal nature short cavity road liquid sample or surface of a wound liquid sample.
2. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; Said natural short cavity road liquid sample comprises oral cavity juice, urethral orifice debris, ight soil and milk, and surface of a wound liquid sample comprises blood, purulent secretion liquid and non-purulent secretion liquid.
3. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; Also comprise developping agent, make an addition on the sample collecting device, with the pH value of regulating the living animal liquid sample and directivity and the flow velocity that guarantees the transportation of living animal liquid sample; Make animal epidemic antigen or antibody immune chromatography reagent strip obtain enough living animal liquid samples, and promote animal epidemic antigen or antibody in the liquid sample to separate voluntarily and carry; This developping agent splendid attire is in the transparent rigid plastic bottle of sealing; Said developping agent is that a kind of pH value is the compound damping fluid of 6.5-8.5, and is formulated in proportion by potassium dihydrogen phosphate, trishydroxymethylaminomethane, sodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20, thimerosal, lauryl sodium sulfate, ethylenediamine tetraacetic acid, deionized water.
4. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; Said plastics containment vessel one end is made as the holding area; The base plate of plastics containment vessel is provided with several air holes in an end of holding area, and its inboard is made as the sample collecting device assembly section away from an end of holding area, and the sample collecting device stationary installation is set on it; The middle part is made as animal epidemic antigen or antibody immune chromatography reagent strip assembly section, and it is provided with the stationary installation that supplies to place animal epidemic antigen or antibody immune chromatography reagent strip.
5. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; Said watch window top is bonded with an eyeglass; The eyeglass marked has " T ", " C " to indicate line, respectively with the detection line and the corresponding setting of nature controlling line of animal epidemic antigen or antibody immune chromatography reagent strip; Eyeglass is processed by transparent rigid plastic.
6. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; The base plate of said plastics containment vessel and panel inboard are provided with coupling arrangement; Be used for base plate is fixedly connected with panel, fixed connection method adopts the method for withholding or adopts the ultrasonic plastic welder welding to be fixedly connected.
7. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that; Said animal epidemic antigen or antibody immune chromatography reagent strip adopt colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology, and this animal epidemic antigen or antibody immune chromatography reagent strip comprise animal epidemic antigen immune chromatography reagent strip, reach animal epidemic antibody immune chromatography reagent strip.
8. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1 is characterized in that said sample collecting device is processed by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure; It is 8%～80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre is cotton fiber, filter paper, sponge or cloth; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, nylon and polyethersulfone and other plastics, glass or resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1; The profile of sample collecting device is table tennis plate or slurry shape, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than sample collecting device total surface area 50%; 20～500 microns of the average pore sizes of capillary micropore matrix, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.21～0.86 gram/cubic centimetre.
9. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1 is characterized in that said sample collecting device is processed by one or more hydrophobic materials, and this hydrophobic material forms capillary micropore matrix structure; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass or resin; The sample collecting device profile is bar-shaped or tabular, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than 50% of sample collecting device total surface area; 20～500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49～0.92 gram/cubic centimetre.
10. living animal eqpidemic disease antigen-antibody fast detecting appearance as claimed in claim 1; It is characterized in that, also comprise bridge, it is a strip; Bridge one end is made as handheld terminal, and an other end is made as installation end so that said living animal eqpidemic disease antigen-antibody fast detecting appearance to be installed; This installation end is provided with notch, the corresponding setting of plastics containment vessel holding area periphery of this notch and living animal eqpidemic disease antigen-antibody fast detecting appearance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104448085A CN102565385A (en) | 2011-12-27 | 2011-12-27 | Rapid detection instrument for epidemic antigen and antibody of living animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104448085A CN102565385A (en) | 2011-12-27 | 2011-12-27 | Rapid detection instrument for epidemic antigen and antibody of living animal |
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| CN103913569A (en) * | 2013-01-08 | 2014-07-09 | 郑兆珉 | Chromatography filter paper type enzyme-linked immunosorbent assay |
| CN105527282A (en) * | 2014-10-16 | 2016-04-27 | 郑兆珉 | Cloth-based biochemical detection device and manufacturing method thereof |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
| CN106769230A (en) * | 2016-12-13 | 2017-05-31 | 李林林 | Urine examination experiment device is adopted by a kind of Smart Verify section |
| WO2021018028A1 (en) * | 2019-08-01 | 2021-02-04 | Hangzhou Biotest Biotech Co., Ltd. | A detection device |
| CN114137198A (en) * | 2021-11-23 | 2022-03-04 | 广州蓝勃生物科技有限公司 | Reagent card and domestic immunity analyzer |
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| CN103913569A (en) * | 2013-01-08 | 2014-07-09 | 郑兆珉 | Chromatography filter paper type enzyme-linked immunosorbent assay |
| CN105527282A (en) * | 2014-10-16 | 2016-04-27 | 郑兆珉 | Cloth-based biochemical detection device and manufacturing method thereof |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
| CN106769230A (en) * | 2016-12-13 | 2017-05-31 | 李林林 | Urine examination experiment device is adopted by a kind of Smart Verify section |
| WO2021018028A1 (en) * | 2019-08-01 | 2021-02-04 | Hangzhou Biotest Biotech Co., Ltd. | A detection device |
| CN114137198A (en) * | 2021-11-23 | 2022-03-04 | 广州蓝勃生物科技有限公司 | Reagent card and domestic immunity analyzer |
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Application publication date: 20120711 Assignee: Shenzhen Kangda Biotechnology Co., Ltd. Assignor: Shenzhen Aisuer Biological Technology Co.,Ltd. Contract record no.: 2012440020126 Denomination of invention: Rapid detection instrument for epidemic antigen and antibody of living animal License type: Exclusive License Record date: 20120525 |
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