CN102559540A - Flavobacterium aquati and application thereof in preparation of L-tryptophan by microbial transformation - Google Patents
Flavobacterium aquati and application thereof in preparation of L-tryptophan by microbial transformation Download PDFInfo
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- CN102559540A CN102559540A CN2011104051066A CN201110405106A CN102559540A CN 102559540 A CN102559540 A CN 102559540A CN 2011104051066 A CN2011104051066 A CN 2011104051066A CN 201110405106 A CN201110405106 A CN 201110405106A CN 102559540 A CN102559540 A CN 102559540A
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Abstract
The invention provides a new strain, namely flavobacterium aquati ZJB-09211 for preparing optically pure L-tryptophan and application of the strain in preparation of the L-tryptophan by microbes. The strain was collected in China Center for Type Culture Collection (CCTCC) on October 17th, 2011, the collection address is Wuhan University in Wuhan of China, the zip code is 430072, and the collection number is CCTCC M2011354. The invention provides the new strain which has stereo selectivity and can be used for preparing the optically pure L-tryptophan; the L-tryptophan with high optical purity can be prepared through the strain; and the strain different from the conventional researched new strains is obtained by screening, so that a foundation is provided for investigating the diversity of microbial strains on the chiral separation aspect and further comparing the difference of different strains on transformation path and reaction mechanism.
Description
(1) technical field
The present invention relates to the new product L-Ntn hydrolase bacterial strain of a strain---flavobacterium aquatile (Flavobacteriumaquati) ZJB-09211, and prepare the application in the L-tryptophane in microbial transformation.
(2) background technology
The L-tryptophane can not synthesize in vivo naturally, need from food, absorb, and is the indispensable amino acid in animal and some fungi vital movements.L-tryptophane content in protein is very low, average content about 1% or still less.The L-tryptophane can regulate proteinic synthetic, regulate immunity and digestive function, increase serotonin metabolism and strengthen cognitive ability etc., therefore in the metabolism of humans and animals, play an important role in growing.These nutrition of L-tryptophane and pharmaceutical use make it be widely used in industries such as medicine, feed and food.
The L-tryptophane can adopt proteolysis method, chemical synthesis-and microbial method produce.Microbial method can be divided into Enzymatic transformation method and direct fermentation substantially:
Nineteen eighty-two, the Nakai (EP 43211) of Japan utilized the L-Ntn hydrolase that the hydrolysis of DL-tryptophyl amine is obtained L-tryptophane and D-tryptophyl amine, and then utilized chemical method to produce the D-tryptophane, and its yield reaches 92%.Komeda and Asano (Enzyme and Microbial Technology; 2008; 43:276-283) cloned from Brevibacterium iodinum in 2008 and had D-amino amides enzyme; This enzyme all has good enzyme work to the amino amides of number of different types, and its glutamine of checking colors also has certain vigor.
(3) summary of the invention
The object of the invention provides the novel bacterial that a strain can be used for preparing optical purity L-tryptophane---flavobacterium aquatile (Flavobacterium aquati) ZJB-09211, and prepare the application in the L-tryptophane in mikrobe.
The technical scheme that the present invention adopts is:
Flavobacterium aquatile (Flavobacterium aquati) ZJB-09211 is preserved in Chinese typical culture collection center, address: China; Wuhan, Wuhan University, postcode: 430072; Preservation date on October 17th, 2011, deposit number CCTCC NO:M 2011354.
The characteristic of this new bacterial strain is following:
Physiological and biochemical property: Gram-negative; Catalase is positive; The oxidation enzyme positive of returning to life from the nether world; Other positive projects are: Histidine assimilation, D-SANMALT-S, acetate, propionic salt assimilation, salicin, the assimilation of DL-lactic acid salt, valerate assimilation, D-melibiose, caprate, L-Ala assimilation, D-glucose, sucrose, proline(Pro), N-acetyl-glycamine, malonate utilization; The project of total negative is: Citrate trianion utilization, itaconate assimilation, D-N.F,USP MANNITOL, pectinose, 3-hydroxyl-butyrates, D-sorbyl alcohol, the assimilation of 4-hydroxy-benzoic acid salt, the assimilation of 3-hydroxy-benzoic acid salt, D-ribose, 2-ketone group glucose, inositol, glycogen, L-rock bath sugar, 5-ketone group glyconate, suberate assimilation, Serine assimilation, gelatine liquefication, L-rhamnosyl, indoles produce.
Mikrobe involved in the present invention is to obtain through following program screening:
1) will be inoculated in the enrichment medium by soil sample and the sewage appearance that adopt back various places in the Zhejiang Province,, cultivated on the shaking table of 150rpm 3 days at 30 ℃, treat that substratum becomes muddiness after, get the 1ml nutrient solution and be transferred in the fresh enrichment medium, cultivated again 3 days.So repeat 4～5 circulations.Enrichment medium is sole carbon, nitrogenous source with 1～2.5g/L (final concentration) tryptophyl amine, adds other material formulas (final concentration): K as follows again
2HPO
412H
2O:1.0～2.5g/L, KH
2PO
4: 1.0～2.0g/L, MgSO
47H
2O:0.2～0.5g/L, FeSO
47H
2O:0.01～0.05g/L prepares with tap water.
2) be applied on the plate culture medium after the last enrichment culture liquid dilution, picking list bacterium colony is to slant preservation.Plate culture medium is the agar that adds 15～20g/L in the enrichment medium.
3) single colony inoculation of picking is to fermention medium, and component is (final concentration) as follows: glucose 10.0～20.0g/L, Carnis Bovis seu Bubali cream 5.0～10.0g/L, peptone 2.0～5.0g/L, NaCl 1.0～3.0g/L, K
2HPO
41.0～2.0g/L, KH
2PO
41.0～2.0g/L, MgSO
40.2～0.5g/L, FeSO
40.01～0.05g/L, ethanamide 2.0～5.0g/L, pH nature (under 121 ℃, sterilization 20min), tap water preparation; Under 25～45 ℃, shake bottle rotating speed a 100～300rpm, cultivate 48h～72h, be suspended in the zero(ppm) water system after centrifugal.Utilize the screening of chirality liquid phase chromatography to produce the bacterial strain of L-Ntn hydrolase then.
4) the product L-Ntn hydrolase bacterial strain to screening through aforesaid method carries out multiple sieve through the hydrolysis conversion reaction.Cultivate the thalline obtain according to the method described above, be suspended in the zero(ppm) water system after centrifugal, add tryptophyl amine, transform as substrate; Content and optical purity with chirality liquid chromatography analysis product.
The concrete operations condition of chiral chromatography is: the U.S. wears peace U3000 liquid chromatograph, chameleon chromatographic working station; U.S. CHIROBIOTIC
TMThe chirality liquid-phase chromatographic column; Flowability is an acetonitrile: 0.5% acetic acid=80: 20, flow velocity are 1mL/min; Sample size 3 μ L, the detection wavelength is 278nm, column temperature is 30 ℃.
Investigated the influence that nutrient media componentses such as carbon source, nitrogenous source, inductor and metals ion are lived to flavobacterium aquatile ZJB-09211 bacterial strain enzyme through Plackett-Burman; The best product enzyme substratum that obtains flavobacterium aquatile ZJB-09211 consists of (final concentration): glucose 9.0～15.0g/L; Yeast powder 6.0～10.0g/L, KH
2PO
40.5～1.5g/L, K
2HPO
40.5～1.5g/L, NaCl 0.5～1g/L, ethanamide 1～3.0g/L, solvent are water.
The optimal culture condition of flavobacterium aquatile ZJB-09211 is: initial pH 6.0～8.5, liquid amount 5～30%, 20～30 ℃ of culture temperature, shaking speed 100～300rpm, incubation time 24～72 hours.
The invention still further relates to described flavobacterium aquatile ZJB-09211 and prepare the application in the L-tryptophane in microbial transformation.
Concrete; Said being applied as: with racemize tryptophyl amine is reaction substrate; Add flavobacterium aquatile ZJB-09211 multiparity enzyme cultivate obtain contain the enzyme somatic cells, carried out conversion reaction 1～30 hour under 28～35 ℃ of shaking table oscillating conditions, reaction solution obtains said L-tryptophane through separation and purification.
Preferably, said product enzyme is cultivated in forming following product enzyme substratum and is carried out: glucose 9.0～15.0g/L, yeast powder 6.0～10.0g/L, KH
2PO
40.5～1.5g/L, K
2HPO
40.5～1.5g/L, NaCl 0.5～1g/L, ethanamide 1～3.0g/L, solvent are water, pH 6.0～8.5.
Preferably, said product enzyme is cultivated under 20～30 ℃, 100～300rpm condition and is carried out incubation time 24～72 hours.
Preferably, said conversion reaction is carried out in the phosphate buffered saline buffer of pH7.0～7.8 or Tris-HCl buffered soln.Preferably, the substrate addition is 0.5～10mg/mL damping fluid, contains enzyme somatic cells addition and counts 10～100mg/mL damping fluid with weight in wet base.
Preferably, for improving transformation efficiency, also being added with volume in the system of said conversion reaction is the solubility promoter methyl alcohol or the ETHYLE ACETATE of damping fluid volume 1～20%.
Beneficial effect of the present invention is mainly reflected in: a kind of novel bacterial that has stereoselectivity, can prepare optical purity L-tryptophane is provided, can have prepared the L-tryptophane of high-optical-purity through this bacterial classification; The present invention obtains the novel bacterial of research different from the past through screening, thereby is that variety and then the difference of comparison different strain on path for transformation and reaction mechanism of investigating the microbial strains aspect the chiral separation provides the foundation.
(4) embodiment
Below in conjunction with specific embodiment the present invention is described further, but protection scope of the present invention is not limited in this:
Embodiment 1: the separation of bacterial strain
Produce the separation that the L-Ntn hydrolase produces bacterium: in the saline water of 9mL 0.85% (w/w), add 1g soil sample, an amount of granulated glass sphere, shake up, make into uniform soil supension; The soil supension of drawing 0.5mL is inoculated in the triangular flask of the 250mL that the 30mL enrichment medium is housed, places 30 ℃, and the shaking table of 150rpm was cultivated 3 days, wait pregnant solution muddiness to occur after, draw 0.5mL again and be transferred in the fresh substratum, continue to cultivate 3 days; After so repeating 4～5 circulations, pregnant solution is diluted a plurality of gradients, be applied on the separating plate, obtain single bacterium colony;
Enrichment medium is sole carbon, nitrogenous source with tryptophyl amine, forms (final concentration): K as follows
2HPO
412H
2O:1g/L, KH
2PO
4: 1g/L, MgSO
47H
2O:0.2g/L, FeSO
47H
2O:0.01g/L, tryptophyl amine: 3g/L, with the tap water preparation, pH is adjusted to 7.0;
Single colony inoculation of picking is in enrichment medium; Take a sample after cultivating 48h, 72h; Detect the corresponding body excessive value (e.e value) of product L-tryptophane with the chirality liquid phase chromatography; Screening obtains a strain e.e. value and surpasses 98% bacterial strain, and this bacterial strain is accredited as flavobacterium aquatile Flavobacterium aquati, is described mikrobe Flavobacterium ZJB-09211 (CCTCC NO:M 2011354).
Embodiment 2: the cultivation of bacterial strain
Slant medium is formed: Carnis Bovis seu Bubali cream 5g/L, peptone 10g/L, NaCl 5g/L, agar 20g/L, tap water preparation back bevel;
Single bacterium colony of picking Flavobacterium ZJB-09211 is seeded to the inclined-plane on flat board, in 30 ℃ of constant incubators, cultivate 48h after, it is subsequent use to put into 4 ℃ of refrigerator preservations.
Embodiment 3: the acquisition of wet thallus cell
Substratum preparation: glucose 10.0g, Carnis Bovis seu Bubali cream 5.0g, peptone 2.0g, KH
2PO
4, 1g, K
2HPO
41g, NaCl 1g, ethanamide 1g, tap water complements to 1L;
250ml shakes bottled liquid measure 25%, and inoculation one ring flavobacterium aquatile ZJB-09211 is in 30 ℃, 150rpm shaking culture 48h; Cultivation end secondary fermentation liquid is centrifugal and use the saline water washed twice, collects the wet thallus cell, is suspended in the zero(ppm) water, gets the wet thallus cell content and is the bacteria suspension of 0.1g/mL, and is subsequent use.
Embodiment 4: the acquisition of wet thallus cell
Substratum preparation: glucose 10.0g, yeast powder 7.0g, KH
2PO
41.0g, K
2HPO
41.0g, NaCl 1g, ethanamide 1.0g, tap water complements to 1L, and pH 8.0;
250ml shakes bottled liquid measure 20%, and inoculation one ring flavobacterium aquatile ZJB-09211 is in 30 ℃, 150rpm shaking culture 48h; Cultivation end secondary fermentation liquid is centrifugal and use the saline water washed twice, collects the wet thallus cell, is suspended in the phosphate buffer soln of 50mM, pH 7.8, gets the wet thallus cell content and is the bacteria suspension of 0.5g/mL, and is subsequent use.
Embodiment 5:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 4mL embodiment 3 gained bacteria suspensions; Add 12mg racemize tryptophyl amine as substrate, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 30 hours.Reaction solution is centrifugal, gets supernatant, through Liquid Detection, and the optical purity 99.5%e.e of product L-tryptophane, transformation efficiency 49.5%.
Embodiment 6:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 1.6mL embodiment 3 gained bacteria suspensions; Add 24mg racemize tryptophyl amine as substrate, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, through Liquid Detection, and the optical purity 99.2%e.e of product L-tryptophane, transformation efficiency 42%.
Embodiment 7:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 6mL embodiment 4 gained bacteria suspensions; Add 24mg racemize tryptophyl amine as substrate, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, through Liquid Detection, and the optical purity 99%e.e of product L-tryptophane, transformation efficiency 48%.
Embodiment 8:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 4mL embodiment 3 gained bacteria suspensions; Add 24mg racemize tryptophyl amine as substrate, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 2 hours.Reaction solution is centrifugal, gets supernatant, the optical purity 99%e.e of product L-tryptophane, transformation efficiency 48%.
Embodiment 9:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 4mL embodiment 4 gained bacteria suspensions; Add 36mg racemize tryptophyl amine as substrate, in 28 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 2 hours.Reaction solution is centrifugal, the optical purity 99%e.e of product L-tryptophane, transformation efficiency 42%.
Embodiment 10:
In the phosphate buffer soln (pH 7.0) of 10mL, 50mM, add 4mL embodiment 3 gained bacteria suspensions; Add 48mg racemize tryptophyl amine as substrate.In 32 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets the supernatant extraction, the optical purity 98.9%e.e of product L-tryptophane, transformation efficiency 49%.
Embodiment 11:
In the Tris-HCl of 10mL, 50mM buffered soln (pH 7.5), add 4mL embodiment 3 gained bacteria suspensions; Add 48mg racemize tryptophyl amine as substrate, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, the optical purity 99%e.e of product L-tryptophane, transformation efficiency 48%.
Embodiment 12:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 4mL embodiment 4 gained bacteria suspensions; Add 48mg racemize tryptophyl amine as substrate and 0.3mL methyl alcohol, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, the optical purity 99.3%e.e of product L-tryptophane, transformation efficiency 49%.
Embodiment 13:
In the phosphate buffer soln (pH 7.8) of 10mL, 50mM, add 4mL embodiment 4 gained bacteria suspensions; Add 48mg racemize tryptophyl amine as substrate and 1mL methyl alcohol, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, the optical purity 99.5%e.e of product L-tryptophane, transformation efficiency 48%.
Embodiment 14:
In the Tris-HCl of 10ml, 50mM buffered soln (pH 7.4), add 4mL embodiment 3 gained bacteria suspensions; Add 48mg racemize tryptophyl amine and 0.6mL ETHYLE ACETATE, in 30 ℃ of water bath with thermostatic control shaking tables oscillatory reaction 1 hour.Reaction solution is centrifugal, gets supernatant, the optical purity 99.2%e.e of product L-tryptophane, transformation efficiency 48%.
Claims (7)
1. flavobacterium aquatile (Flavobacterium aquati) ZJB-09211 is preserved in Chinese typical culture collection center, address: China; Wuhan, Wuhan University, postcode: 430072; Preservation date on October 17th, 2011, deposit number CCTCC NO:M 2011354.
2. flavobacterium aquatile ZJB-09211 as claimed in claim 1 prepares the application in the L-tryptophane in microbial transformation.
3. application as claimed in claim 2; It is characterized in that said being applied as: with racemize tryptophyl amine is reaction substrate; Add flavobacterium aquatile ZJB-09211 multiparity enzyme cultivate obtain contain the enzyme somatic cells; Carried out conversion reaction 1～30 hour under 28～35 ℃ of shaking table oscillating conditions, reaction solution obtains said L-tryptophane through separation and purification.
4. application as claimed in claim 3, it is characterized in that said product enzyme is cultivated in forming following product enzyme substratum carries out: glucose 9.0～15.0g/L, yeast powder 6.0～10.0g/L, KH
2PO
40.5～1.5g/L, K
2HPO
40.5～1.5g/L, NaCl 0.5～1g/L, ethanamide 1～3.0g/L, solvent are water, pH 6.0～8.5.
5. application as claimed in claim 3, it is characterized in that said product enzyme is cultivated under 20～30 ℃, 100～300rpm condition carries out incubation time 24～72 hours.
6. application as claimed in claim 3 is characterized in that said conversion reaction carries out in the phosphate buffered saline buffer of pH7.0～7.8 or Tris-HCl buffered soln.
7. application as claimed in claim 6 is characterized in that: also be added with methyl alcohol or ETHYLE ACETATE that volume is a damping fluid volume 1～20% in the system of said conversion reaction.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936571A (en) * | 2012-11-05 | 2013-02-20 | 浙江工业大学 | Geotrichum candidum ZJB-09214 and application in biocatalysis synthesis of L-tryptophan |
| CN110305819A (en) * | 2019-08-16 | 2019-10-08 | 南京工业大学 | One plant of feather efficient degrading bacterial strain and its application |
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| US4016037A (en) * | 1975-10-15 | 1977-04-05 | Ajinomoto Co., Inc. | Method for producing L-amino acid |
| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
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2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4016037A (en) * | 1975-10-15 | 1977-04-05 | Ajinomoto Co., Inc. | Method for producing L-amino acid |
| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
Non-Patent Citations (1)
| Title |
|---|
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936571A (en) * | 2012-11-05 | 2013-02-20 | 浙江工业大学 | Geotrichum candidum ZJB-09214 and application in biocatalysis synthesis of L-tryptophan |
| CN102936571B (en) * | 2012-11-05 | 2014-03-26 | 浙江工业大学 | Geotrichum candidum ZJB-09214 and application in biocatalysis synthesis of L-tryptophan |
| CN110305819A (en) * | 2019-08-16 | 2019-10-08 | 南京工业大学 | One plant of feather efficient degrading bacterial strain and its application |
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