CN102559498B - Nutritional agent for culturing microorganisms and microorganism culturing method - Google Patents
Nutritional agent for culturing microorganisms and microorganism culturing method Download PDFInfo
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- CN102559498B CN102559498B CN 201110457552 CN201110457552A CN102559498B CN 102559498 B CN102559498 B CN 102559498B CN 201110457552 CN201110457552 CN 201110457552 CN 201110457552 A CN201110457552 A CN 201110457552A CN 102559498 B CN102559498 B CN 102559498B
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Abstract
The invention provides a nutritional agent for culturing microorganisms. The nutritional agent consists of the following components in parts by weight: 80-120 parts of glucose, 15-20 parts of urea, 2-5 parts of disodium hydrogen phosphate, 2-5 parts of monopotassium phosphate and 0.1-0.3 part of magnesium sulfate. The invention further provides a method for culturing organisms in sewage by using the nutritional agent. According to the nutritional agent and the microorganism culturing method provided by the invention, quick propagation of microorganisms in sewage can be promoted, adaption of microorganisms to the environment is accelerated, a large quantity of microorganisms needed in a sewage treatment process can be cultured within an extremely short period of time, and an optimal treatment effect is achieved.
Description
Technical field
The present invention relates to a kind of nutrition agent of culturing micro-organisms, be specifically related to the nutrition agent of culturing micro-organisms in a kind of sewage treatment process, and utilize this nutrition agent to cultivate method of microorganism in the sewage.
Background technology
In order to overcome the problem of microorganism deficiency in the sewage disposal initial stage water, at present general Sewage treatment systems all can have the debug phase of certain hour before formal operation, so that microorganism shakes down and breeds in the water, draw mud to start biochemical system outside normal utilization of the debug phase of general Sewage treatment systems, this must bring following shortcoming: the anaerobic system start-up period is long, Load lifting is very slow, and the cultivation domestication time is generally 2～6 months; Aerobic system is under many factors, and initial failure period also got more than 1 month, brings inconvenience for producer and engineering side, consumes simultaneously a large amount of manpower and materials.For the waste water of some high densitys, high ammonia nitrogen, high salinity, draw mud often to need slowly to cultivate domestication by former water is diluted outward, brought extra cost to engineering, prolonged start-up period, success ratio is low, can not satisfy the quick operation demand of system.For the poor waste water of biodegradability, as contain the waste water of the materials such as benzene class, dyestuff, oils, conventional culturing sludge need to add a large amount of external carbon sources, and speed is slow, and cost is high, and removal effect is undesirable, and difficulty up to standard is large.
Microorganism reaches sufficient amount with the pollutent in disposing of sewage in the water in order to make, the contriver is through constantly research, propose a kind of microorganism (bacterial classification) and cultivate used nutrition agent, can promote the rapid breeding of microorganism, accelerate microorganism to the adaptation of environment, within the extremely short time, can turn out needed microorganism in a large amount of sewage treatment process, and make treatment effect reach best.
Summary of the invention
One object of the present invention is: a kind of nutrition agent for culturing micro-organisms is provided, can promote the rapid breeding of microorganism in sewage, accelerate microorganism to the adaptation of environment, within the extremely short time, can turn out needed microorganism in a large amount of sewage treatment process.
Another object of the present invention is: a kind of microbial culture method is provided, can within the extremely short time, can turns out needed microorganism in a large amount of sewage treatment process, and make treatment effect reach best.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of nutrition agent for culturing micro-organisms is provided, is formed by the component of following molar ratio: 80～120 glucose, 15～20 urea, 2～5 Sodium phosphate dibasic, 2～5 potassium primary phosphate and 0.1～0.3 sal epsom.
Nutrition agent of the present invention, preferably the component by following molar ratio forms: 100 glucose, 18 urea, 3 Sodium phosphate dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
Nutrition agent of the present invention can be mixed by commercially available various feed composition and get.
Described nutrition agent wherein, glucose can be microorganism growth carbon source is provided, urea can provide nitrogenous source, Sodium phosphate dibasic and potassium primary phosphate and sal epsom can provide the trace elements such as phosphorus and potassium, sodium, sulphur, magnesium.
The microbial growth breeding needs various nutrition.Aerobic microbiological colony the best requires BOD5 (C): N: P=100: 5: 1 (mol ratio), anaerobion colony the best requires BOD5 (C): N: P=100: 6: 1 (mol ratio), wherein, anaerobion is more responsive to the variation of nutrition composition in the environment, and aerobic microbiological is lower to the change sensitivity of nutrition composition.The growth and breeding of comprehensive aerobic microbiological and anaerobion, in the nutrition agent prescription of the present invention, C: N: P=100: 5.8: 1 (mol ratio) all is in more satisfactory scope to the growth and breeding nutritional condition of aerobic microbiological and anaerobion, the growth and breeding of aerobic microbiological and anaerobion all is in comparatively desirable environment, is conducive to the rapid breeding of aerobic microbiological and anaerobion most.
The present invention also provides method for culturing microbes in a kind of sewage, at the operation of sewage disposal system initial stage, in sewage, add the described nutrition agent of 150～300g/ ton, under the envrionment conditions of routine, continuous aeration 20～30 hours is after continuous aeration is finished, adopt the mode of intermittent aeration, aeration 5～10 minutes stopped exposing to the sun 10～20 minutes, and so intermittent aeration is 8～12 days.
The preferred 250g/ ton of described nutrition agent dosage.
Preferred 24 hours of described continuous aeration time.
The preferred aeration of described intermittent aeration mode 7 minutes stopped exposing to the sun 14 minutes.
Preferred 9 days of described intermittent aeration time.
At system's initial operating stage, adopt cultural method of the present invention can promote simultaneously the rapid breeding of aerobic microbiological in the sewage, facultative aerobic microbiological and anaerobion.Compare with existing cultural method, the efficient cultural method of this kind, incubation time microbe population short, that turn out is many, with strong points, can be widely used in various sewage treatment process.
Adopt microbial culture method of the present invention, Chinese Marine University's sanitary sewage disposal engineering, Zhong County, the Chongqing sanitary sewage disposal engineering of operation, Vietnam's cassava alcohol waste water treatment engineering, rice fragrance of a flower wastewater treatment project, the tenth of the twelve Earthly Branches sun clothing wastewater treatment project, luxuriant rich cashmere treatment of dyeing wastewater engineering, all reached in the short period of time best operating performance, more traditional sludge acclimatization method time obviously shortens.
Embodiment
Embodiment 1.
A kind of nutrition agent for cultivating the sewage microorganism is comprised of the component of following molar ratio:
80 glucose, 20 urea, 2 Sodium phosphate dibasic, 5 potassium primary phosphate and 0.3 sal epsom.
Above-mentioned each feed composition mixed namely obtain nutrition agent of the present invention.
Embodiment 2.
A kind of nutrition agent for cultivating the sewage microorganism is comprised of the component of following molar ratio:
120 glucose, 15 urea, 5 Sodium phosphate dibasic, 2 potassium primary phosphate and 0.1 sal epsom.
Above-mentioned each feed composition mixed namely obtain nutrition agent of the present invention.
Embodiment 3.
A kind of nutrition agent for cultivating the sewage microorganism is comprised of the component of following molar ratio:
100 glucose, 18 urea, 3 Sodium phosphate dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
Above-mentioned each feed composition mixed namely obtain nutrition agent of the present invention.
Embodiment 4.
Method for culturing microbes in a kind of sewage, at the operation of sewage disposal system initial stage, in sewage, add the nutrition agent that 250g/ ton embodiment 3 prepares, under the envrionment conditions of routine, continuous aeration 24 hours is after continuous aeration is finished, adopt the mode of intermittent aeration, aeration 7 minutes stopped exposing to the sun 14 minutes, and so intermittent aeration is 9 days.
Embodiment 5.
Method for culturing microbes in a kind of sewage, at the operation of sewage disposal system initial stage, in sewage, add the nutrition agent that 300g/ ton embodiment 1 prepares, under the envrionment conditions of routine, continuous aeration 20 hours is after continuous aeration is finished, adopt the mode of intermittent aeration, aeration 5 minutes stopped exposing to the sun 15 minutes, and so intermittent aeration is 12 days.
Embodiment 6.
The microorganism of using microbial culture method of the present invention that the Chinese Sea Dormitory on Campus is arranged in the sanitary sewage every day is cultivated:
Test used sewage and arranged sanitary sewage every day by the Chinese Marine University students' dormitory.Experiment is divided into two groups, and control group adopts traditional microbial acclimation method to cultivate, and experimental group adopts the cultural method of embodiment 4 to cultivate.Two groups of experiments are carried out under identical environment.In culturing process, carry out microscopy by microscope every day, analyzes the proterties of mud and the variation tendency of miniature organism kind and quantity.The result is as follows:
In the initial several days domestication process, protozoa peritricha (Peritricha) sessile form ciliate quantity has obvious minimizing, and what substitute big amount appearance is Holotricha (Holotricha) swimming type ciliate, and has the worms on a sucker of part to occur.Along with the propelling of incubation time, when the miniature organism in the mud adapted to new water quality and growing environment gradually, the ciliate of sessile form was restarted a large amount of appearance, and the companion has a small amount of swimming type ciliate.In the culturing sludge later stage, the sessile form ciliate has comparative advantage, the miniature organisms such as roaming worm, wheel animalcule in succession occur, and this moment, the cultivation of mud was finished substantially, and active sludge forms the macrobead floc sedimentation comprehensively, and close structure, and settling property is good.
Find by contrast: above-mentioned phenomenon appears in experimental group, and namely the sessile form ciliate has comparative advantage, and when the miniature organism such as roaming worm, wheel animalcule in succession occurring, the ciliate of control group sessile form begins a large amount of appearance, and companion has a small amount of swimming type ciliate appearance.And when experiment proceeded to 10 days left and right sides, the characteristic of two experimental group mud reached wherein situation such as the following table 1 of superior microorganism:
Table 1
Can be found out that by above-mentioned experiment can the increase sharply quantity of microorganism in the treatment system of microbial culture method of the present invention is accelerated microorganism to the adaptation of environment, thereby made treatment system just can reach best treatment effect within the extremely short time.
Claims (7)
1. nutrition agent that is used for culturing micro-organisms is characterized in that it is comprised of the component of following molar ratio: 80～120 glucose, 15～20 urea, 2～5 Sodium phosphate dibasic, 2～5 potassium primary phosphate and 0.1～0.3 sal epsom.
2. the nutrition agent of culturing micro-organisms claimed in claim 1 is characterized in that, it is comprised of the component of following molar ratio: 100 glucose, 18 urea, 3 Sodium phosphate dibasic, 3 potassium primary phosphate and 0.2 sal epsom.
3. method for culturing microbes in the sewage, it is characterized in that: at the operation of sewage disposal system initial stage, in sewage, add 150～300g/ ton nutrition agent claimed in claim 1, under the envrionment conditions of routine, continuous aeration 20～30 hours is after continuous aeration is finished, adopt the mode of intermittent aeration, aeration 5～10 minutes stopped exposing to the sun 10～20 minutes, and so intermittent aeration is 8～12 days.
4. cultural method claimed in claim 3, it is characterized in that: described nutrition agent dosage is the 250g/ ton.
5. cultural method claimed in claim 3, it is characterized in that: the described continuous aeration time is 24 hours.
6. cultural method claimed in claim 3, it is characterized in that: described intermittent aeration mode is aeration 7 minutes, stops exposing to the sun 14 minutes.
7. cultural method claimed in claim 3, it is characterized in that: the described intermittent aeration time is 9 days.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104419643A (en) * | 2013-09-03 | 2015-03-18 | 大连大孤山污水处理有限公司 | Culture process of novel biological flora |
| CN104150608B (en) * | 2014-07-31 | 2016-03-09 | 湖南永清水务有限公司 | A kind of high-salt wastewater biochemical processing process |
| CN104893977A (en) * | 2015-05-13 | 2015-09-09 | 江苏恒丰科技有限公司 | Comprehensive microbe culture method |
| CN105779331A (en) * | 2016-03-07 | 2016-07-20 | 重庆坤泉环境工程有限公司 | Biological strain nutritional agent and preparation method and application thereof |
| CN106430555A (en) * | 2016-10-09 | 2017-02-22 | 北京恒蓝环保科学研究院有限责任公司 | Nutrient for aerobic treatment, aerobic treatment activated sludge culture method and device |
| CN106630180B (en) * | 2016-11-10 | 2019-05-28 | 中国海洋石油集团有限公司 | Improve the preparation method of the slow-release nutrient of chemical production wastewater Biochemical Treatment |
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| US4246100A (en) * | 1979-10-22 | 1981-01-20 | Bio-Humus, Inc. | Composition and method for the treatment of sewage |
| WO1999050193A1 (en) * | 1998-03-31 | 1999-10-07 | Samsung Engineering Co., Ltd. | Wastewater treatment method for removing organic matter and nitrogen, carrier used thereof and method for manufacturing the carrier |
| CN1594140A (en) * | 2004-03-02 | 2005-03-16 | 戴双建 | Domestic sewage treatment method by intermittent aeration contact oxidation process |
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| US4246100A (en) * | 1979-10-22 | 1981-01-20 | Bio-Humus, Inc. | Composition and method for the treatment of sewage |
| WO1999050193A1 (en) * | 1998-03-31 | 1999-10-07 | Samsung Engineering Co., Ltd. | Wastewater treatment method for removing organic matter and nitrogen, carrier used thereof and method for manufacturing the carrier |
| CN1594140A (en) * | 2004-03-02 | 2005-03-16 | 戴双建 | Domestic sewage treatment method by intermittent aeration contact oxidation process |
Non-Patent Citations (4)
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| MBR中MLSS的变化对处理效果的影响;由昆 等;《沈阳建筑大学学报(自然科学版)》;20060930;第22卷(第5期);825-828页 * |
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| 由昆 等.MBR中MLSS的变化对处理效果的影响.《沈阳建筑大学学报(自然科学版)》.2006,第22卷(第5期),825-828页. |
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