CN102534009B

CN102534009B - SNP (Single Nucleotide Polymorphism) marker correlated to assistant diagnosis of primary lung cancer and application thereof

Info

Publication number: CN102534009B
Application number: CN201210013090.9A
Authority: CN
Inventors: 沈洪兵; 胡志斌; 马红霞; 靳光付; 董静; 孙重期
Original assignee: Nanjing Medical University
Current assignee: Nanjing Jiangbei New Area Biomedical Public Service Platform Co., Ltd.
Priority date: 2012-01-16
Filing date: 2012-01-16
Publication date: 2014-03-19
Anticipated expiration: 2032-01-16
Also published as: CN102534009A

Abstract

The invention belongs to the fields of gene engineering and tumor medicines, and discloses an SNP (Single Nucleotide Polymorphism) marker correlated to assistant diagnosis of primary lung cancer and application thereof. The marker is a combination of the following 63 SNP: rs107401, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809 and the like. The marker can be used for preparing an assistant diagnosis kit for the primary lung cancer.

Description

A kind of SNP mark and the application thereof relevant to primary lung cancer auxiliary diagnosis

Invention field

The invention belongs to genetically engineered and tumour medical field, relate to a kind of SNP mark and the application thereof relevant to primary lung cancer auxiliary diagnosis.

Background technology

Lung cancer is the current whole world maximum malignant tumour of number of getting involved.According to IARC (IARC), estimate lung cancer new cases 160.7 ten thousand people in 2008, dead 137.5 ten thousand people, the first place that occupies the interior tumor incidence of global range and mortality ratio.Change due to tobacco consumption, environmental pollution and the mode of life of sustainable growth, recent two decades comes, China's lung cancer morbidity and death are always in rising trend, only between 2000 to 2005, the number of the infected of lung cancer in China and because the number of lung cancer death has increased respectively 120,000 and 100,000, according to estimates, within 2008, China's male lung cancer sickness rate is 45.9/10 ten thousand, women is 21.3/10 ten thousand, higher than the mean level (ML) in the world and approach flourishing industrialized country's level.The famous oncologist R.Peto prophesy of Britain: if China controls smoking and atmospheric pollution not in time, will, over 1,000,000, become the first in the world lung cancer big country to the annual lung cancer of China in 2025.As can be seen here, lung cancer is one of important diseases threatening the whole world and China resident life and health, is great public health problem urgently to be resolved hurrily.

Since the sixties in 20th century, Doll and Hill found the relation of smoking and lung cancer first by case-control study and cohort study, tobacco smoke exposure has been considered to the main environmental risk factors that lung cancer occurs.Research shows, the comparable non-smoker of risk that lung cancer occurs smoker exceeds 10 times.Studies show that of lung cancer clustering of disease in family, inherited genetic factors is played an important role equally in the generation of lung cancer.Twin study shows, lung cancer is the coefficient result of nature-nurture.Epidemiological study shows, more than 80% lung cancer can be laid the blame on for tobacco smoke exposure, but only has the smoker less than 20% that lung cancer occurs, and illustrates under equal environmental exposure, and the individuality with different genetic backgrounds is different to the susceptibility of lung cancer.Wherein mononucleotide polymorphic (single nucleotide polymorphism, SNP) plays an important role.

SNP is the DNA polymorphism that a class causes based on single nucleotide variation, by hereditary educational circles, is called third generation genetic marker.Mainly refer to the DNA sequence polymorphism being caused by the variation in genome nucleotide level, comprise single base transition, transversion, and the insertion/deletion of single base etc.It is the class polymorphism mark the most extensively existing in genome, accounts for about 90%.These Genomic changes can cause the difference of phenotype between individuality and Different Individual to disease, particularly the susceptibility of complex disease and the difference to environmental factors, drug reaction.Therefore SNP causes the individual important hereditary basis to common disease morbidity and prognosis susceptibility difference.Utilize the generation of the SNP spectrum predictive disease of disease-susceptible humans, not only sensitive, accurate and quick, have broad application prospects, and the structure of predicting spectrum by SNP, can also make prospective diagnosis to disease.In recent years, utilize the generation development of SNP predictive disease to become clinical and study hotspot researcher, the using value first meeting clue in the common major disease prediction such as tumour and cardiovascular and cerebrovascular diseases.

Yet, also SNP is not applied at present to the report of primary pulmonary cancerous diagnose, if can filter out the SNP of primary lung cancer susceptible as biomarker, and develop corresponding diagnostic kit, to China's primary pulmonary cancerous diagnose present situation, will be once strong promotion, also for its drug screening, evaluating drug effect and targeted therapy have been opened up new approach.

Summary of the invention

The object of the invention is for above-mentioned technical problem, propose a kind of SNP mark and the application thereof relevant to primary lung cancer auxiliary diagnosis.

Second object of the present invention is to provide the Auele Specific Primer of above-mentioned SNP mark.

The 3rd object of the present invention is to provide the specificity fluorescent probe pair of above-mentioned SNP mark.

The 4th object of the present invention is to provide the application in preparing primary lung cancer auxiliary diagnostic box of above-mentioned SNP mark and Auele Specific Primer and specificity fluorescent probe thereof.

The 5th object of the present invention is to provide primary lung cancer auxiliary diagnostic box.

Contriver by separated and research Patients With Primary Lung Cancer and with the normal healthy controls peripheral blood DNA of its age-matched in single nucleotide polymorphism, find one group with the high specific of primary lung cancer height correlation and the SNP of susceptibility, and develop the primary lung cancer auxiliary diagnostic box that can be convenient to clinical application, for examination and the diagnosis of primary lung cancer provides Data support, for finding to have the new small molecule drug provision Data support of potential therapeutic value.

The object of the invention is to realize by following technical proposal:

A SNP mark relevant to primary lung cancer auxiliary diagnosis, this mark is rs107401, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2285947, rs2895680, rs3969178, rs4693311, rs4766994, rs4894545, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, the combination of rs6043705 and rs11699642.

The Auele Specific Primer of described SNP mark, these primers are:

The primer sequence of rs107401 is SEQ ID No:1 and SEQ ID No:2; The primer sequence of rs465498 is SEQID No:5 and SEQ ID No:6; The primer sequence of rs663414 is SEQ ID No:9 and SEQ ID No:10; The primer sequence of rs622917 is SEQ ID No:13 and SEQ ID No:14; The primer sequence of rs636586 is SEQID No:17 and SEQ ID No:18; The primer sequence of rs741061 is SEQ ID No:21 and SEQ ID No:22; The primer sequence of rs855641 is SEQ ID No:25 and SEQ ID No:26; The primer sequence of rs948783 is SEQID No:29 and SEQ ID No:30; The primer sequence of rs1544694 is SEQ ID No:33 and SEQ ID No:34; The primer sequence of rs1663689 is SEQ ID No:37 and SEQ ID No:38; The primer sequence of rs2030378 is SEQID No:41 and SEQ ID No:42; The primer sequence of rs2225588 is SEQ ID No:45 and SEQ ID No:46; The primer sequence of rs2317683 is SEQ ID No:49 and SEQ ID No:50; The primer sequence of rs2395166 is SEQID No:53 and SEQ ID No:54; The primer sequence of rs2405271 is SEQ ID No:57 and SEQ ID No:58; The primer sequence of rs2788713 is SEQ ID No:61 and SEQ ID No:62; The primer sequence of rs3130975 is SEQID No:65 and SEQ ID No:66; The primer sequence of rs4344933 is SEQ ID No:69 and SEQ ID No:70; The primer sequence of rs4488809 is SEQ ID No:73 and SEQ ID No:74; The primer sequence of rs4493136 is SEQ ID No:77 and SEQ ID No:78; The primer sequence of rs4669621 is SEQ ID No:81 and SEQ IDNo:82; The primer sequence of rs4697406 is SEQ ID No:85 and SEQ ID No:86; The primer sequence of rs4749794 is SEQ ID No:89 and SEQ ID No:90; The primer sequence of rs4809957 is SEQ ID No:93 and SEQ ID No:94; The primer sequence of rs6102795 is SEQ ID No:97 and SEQ ID No:98; The primer sequence of rs6490820 is SEQ ID No:101 and SEQ ID No:102; The primer sequence of rs9317873 is SEQ IDNo:105 and SEQ ID No:106; The primer sequence of rs9373105 is SEQ ID No:109 and SEQ IDNo:110; The primer sequence of rs9397087 is SEQ ID No:113 and SEQ ID No:114; The primer sequence of rs9463490 is SEQ ID No:117 and SEQ ID No:118; The primer sequence of rs10066153 is SEQ IDNo:121 and SEQ ID No:122; The primer sequence of rs11144475 is SEQ ID No:125 and SEQ IDNo:126; The primer sequence of rs11155286 is SEQ ID No:129 and SEQ ID No:130; The primer sequence of rs11264579 is SEQ ID No:133 and SEQ ID No:134; The primer sequence of rs11907465 is SEQ IDNo:137 and SEQ ID No:138; The primer sequence of rs12050469 is SEQ ID No:141 and SEQ IDNo:142; The primer sequence of rs12992554 is SEQ ID No:145 and SEQ ID No:146; The primer sequence of rs13000907 is SEQ ID No:149 and SEQ ID No:150; The primer sequence of rs16883398 is SEQ IDNo:153 and SEQ ID No:154; The primer sequence of rs16959825 is SEQ ID No:157 and SEQ IDNo:158; The primer sequence of rs17058190 is SEQ ID No:161 and SEQ ID No:162; The primer sequence of rs17075390 is SEQ ID No:165 and SEQ ID No:166; The primer sequence of rs17728461 is SEQ IDNo:169 and SEQ ID No:170; The primer sequence of rs1014588 is SEQ ID No:173 and SEQ IDNo:174; The primer sequence of rs1039119 is SEQ ID No:177 and SEQ ID No:178; The primer sequence of rs1726744 is SEQ ID No:181 and SEQ ID No:182; The primer sequence of rs2285947 is SEQ ID No:185 and SEQ ID No:186; The primer sequence of rs2895680 is SEQ ID No:189 and SEQ ID No:190; The primer sequence of rs3969178 is SEQ ID No:193 and SEQ ID No:194; The primer sequence of rs4693311 is SEQ ID No:197 and SEQ ID No:198; The primer sequence of rs4766994 is SEQ ID No:201 and SEQ IDNo:202; The primer sequence of rs4894545 is SEQ ID No:205 and SEQ ID No:206; The primer sequence of rs4940849 is SEQ ID No:209 and SEQ ID No:210; The primer sequence of rs6490294 is SEQ ID No:213 and SEQ ID No:214; The primer sequence of rs6780858 is SEQ ID No:217 and SEQ ID No:218; The primer sequence of rs9439527 is SEQ ID No:221 and SEQ ID No:222; The primer sequence of rs9556016 is SEQ ID No:225 and SEQ ID No:226; The primer sequence of rs12002185 is SEQ ID No:229 and SEQID No:230; The primer sequence of rs12793370 is SEQ ID No:233 and SEQ ID No:234; The primer sequence of rs1549058 is SEQ ID No:237 and SEQ ID No:238; The primer sequence of rs2736100 is SEQ IDNo:241 and SEQ ID No:242; The primer sequence of rs6043705 is SEQ ID No:245 and SEQ IDNo:246; The primer sequence of rs11699642 is SEQ ID No:249 and SEQ ID No:250.

The specificity fluorescent probe pair of described SNP mark, these fluorescent probes are:

The fluorescent probe of rs107401 is SEQ ID No:3 and SEQ ID No:4 to sequence; The fluorescent probe of rs465498 is SEQ ID No:7 and SEQ ID No:8 to sequence; The fluorescent probe of rs663414 is SEQ ID No:11 and SEQ ID No:12 to sequence; The fluorescent probe of rs622917 is SEQ ID No:15 and SEQ ID No:16 to sequence; The fluorescent probe of rs636586 is SEQ ID No:19 and SEQ ID No:20 to sequence; The fluorescent probe of rs741061 is SEQ ID No:23 and SEQ ID No:24 to sequence; The fluorescent probe of rs855641 is SEQ ID No:27 and SEQ ID No:28 to sequence; The fluorescent probe of rs948783 is SEQ ID No:31 and SEQ ID No:32 to sequence; The fluorescent probe of rs1544694 is SEQ ID No:35 and SEQ ID No:36 to sequence; The fluorescent probe of rs1663689 is SEQ ID No:39 and SEQ ID No:40 to sequence; The fluorescent probe of rs2030378 is SEQ ID No:43 and SEQ IDNo:44 to sequence; The fluorescent probe of rs2225588 is SEQ ID No:47 and SEQ ID No:48 to sequence; The fluorescent probe of rs2317683 is SEQ ID No:51 and SEQ ID No:52 to sequence; The fluorescent probe of rs2395166 is SEQ IDNo:55 and SEQ ID No:56 to sequence; The fluorescent probe of rs2405271 is SEQ ID No:59 and SEQ ID No:60 to sequence; The fluorescent probe of rs2788713 is SEQ ID No:63 and SEQ ID No:64 to sequence; The fluorescent probe of rs3130975 is SEQ ID No:67 and SEQ ID No:68 to sequence; The fluorescent probe of rs4344933 is SEQ ID No:71 and SEQ ID No:72 to sequence; The fluorescent probe of rs4488809 is SEQ ID No:75 and SEQ ID No:76 to sequence; The fluorescent probe of rs4493136 is SEQ ID No:79 and SEQ ID No:80 to sequence; The fluorescent probe of rs4669621 is SEQ ID No:83 and SEQ ID No:84 to sequence; The fluorescent probe of rs4697406 is SEQ IDNo:87 and SEQ ID No:88 to sequence; The fluorescent probe of rs4749794 is SEQ ID No:91 and SEQ ID No:92 to sequence; The fluorescent probe of rs4809957 is SEQ ID No:95 and SEQ ID No:96 to sequence; The fluorescent probe of rs6102795 is SEQ ID No:99 and SEQ ID No:100 to sequence; The fluorescent probe of rs6490820 is SEQ IDNo:103 and SEQ ID No:104 to sequence; The fluorescent probe of rs9317873 is SEQ ID No:107 and SEQ IDNo:108 to sequence; The fluorescent probe of rs9373105 is SEQ ID No:111 and SEQ ID No:112 to sequence; The fluorescent probe of rs9397087 is SEQ ID No:115 and SEQ ID No:116 to sequence; The fluorescent probe of rs9463490 is SEQ ID No:119 and SEQ ID No:120 to sequence; The fluorescent probe of rs10066153 is SEQ ID No:123 and SEQ ID No:124 to sequence; The fluorescent probe of rs11144475 is SEQ ID No:127 and SEQ ID No:128 to sequence; The fluorescent probe of rs11155286 is SEQ ID No:131 and SEQ ID No:132 to sequence; The fluorescent probe of rs11264579 is SEQ ID No:135 and SEQ ID No:136 to sequence; The fluorescent probe of rs11907465 is SEQID No:139 and SEQ ID No:140 to sequence; The fluorescent probe of rs12050469 is SEQ ID No:143 and SEQ IDNo:144 to sequence; The fluorescent probe of rs12992554 is SEQ ID No:147 and SEQ ID No:148 to sequence; The fluorescent probe of rs13000907 is SEQ ID No:151 and SEQ ID No:152 to sequence; The fluorescent probe of rs16883398 is SEQ ID No:155 and SEQ ID No:156 to sequence; The fluorescent probe of rs16959825 is SEQ ID No:159 and SEQ ID No:160 to sequence; The fluorescent probe of rs17058190 is SEQ ID No:163 and SEQ ID No:164 to sequence; The fluorescent probe of rs17075390 is SEQ ID No:167 and SEQ ID No:168 to sequence; The fluorescent probe of rs17728461 is SEQ ID No:171 and SEQ ID No:172 to sequence; The fluorescent probe of rs1014588 is SEQID No:175 and SEQ ID No:176 to sequence; The fluorescent probe of rs1039119 is SEQ ID No:179 and SEQ IDNo:180 to sequence; The fluorescent probe of rs1726744 is SEQ ID No:183 and SEQ ID No:184 to sequence; The fluorescent probe of rs2285947 is SEQ ID No:187 and SEQ ID No:188 to sequence; The fluorescent probe of rs2895680 is SEQ ID No:191 and SEQ ID No:192 to sequence; The fluorescent probe of rs3969178 is SEQ ID No:195 and SEQ ID No:196 to sequence; The fluorescent probe of rs4693311 is SEQ ID No:199 and SEQ ID No:200 to sequence; The fluorescent probe of rs4766994 is SEQ ID No:203 and SEQ ID No:204 to sequence; The fluorescent probe of rs4894545 is SEQ ID No:207 and SEQ ID No:208 to sequence; The fluorescent probe of rs4940849 is SEQ IDNo:211 and SEQ ID No:212 to sequence; The fluorescent probe of rs6490294 is SEQ ID No:215 and SEQ IDNo:216 to sequence; The fluorescent probe of rs6780858 is SEQ ID No:219 and SEQ ID No:220 to sequence; The fluorescent probe of rs9439527 is SEQ ID No:223 and SEQ ID No:224 to sequence; The fluorescent probe of rs9556016 is SEQ ID No:227 and SEQ ID No:228 to sequence; The fluorescent probe of rs12002185 is SEQ ID No:231 and SEQ ID No:232 to sequence; The fluorescent probe of rs12793370 is SEQ ID No:235 and SEQ ID No:236 to sequence; The fluorescent probe of rs1549058 is SEQ ID No:239 and SEQ ID No:240 to sequence; The fluorescent probe of rs2736100 is SEQ ID No:243 and SEQ ID No:244 to sequence; The fluorescent probe of rs6043705 is SEQ IDNo:247 and SEQ ID No:248 to sequence; The fluorescent probe of rs11699642 is SEQ ID No:251 and SEQ IDNo:252 to sequence.

The application of described SNP mark in preparing primary lung cancer auxiliary diagnostic box.

The application of the Auele Specific Primer of described SNP mark in preparing primary lung cancer auxiliary diagnostic box.

The specificity fluorescent probe of described SNP mark is to the application in preparing primary lung cancer auxiliary diagnostic box.

A primary lung cancer auxiliary diagnostic box, this test kit is for detection of rs107401 in peripheral blood DNA, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2285947, rs2895680, rs3969178, rs4693311, rs4766994, rs4894545, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, rs6043705 and rs11699642.

Described diagnostic kit, the Auele Specific Primer that this test kit contains above-mentioned SNP mark and/or specificity fluorescent probe.

Described diagnostic kit, the Auele Specific Primer of the SNP mark that this test kit contains is:

Described diagnostic kit, the specificity fluorescent probe of the SNP mark that this test kit contains is to being:

Described diagnostic kit, this test kit can also comprise that PCR reacts conventional enzyme and reagent, as Taq enzyme, dNTP mixed solution, Mgcl2 solution, deionized water etc.; Can also contain standard substance and/or reference substance.

Specifically, the technical scheme that the present invention deals with problems comprises: (1) sets up sample storehouse and the database of unified standard: with Standard operation procedure SOP (SOP), gather standard compliant blood sample, demography data and clinical data that systematic collection is complete.(2) genotype detection: select primary lung cancer case, with contrasting of primary lung cancer case age, gender matched, utilize high-density SNP chip, find out the SNP relevant to primary lung cancer in full genome range.(3) to the associated SNP of the positive filtering out, further in other sample, detect, with the stability that judges that it is associated.(4) development of primary lung cancer auxiliary diagnostic box: according to the genotype distribution frequency SNP that there were significant differences exploitation SNP auxiliary diagnostic box in primary lung cancer case and normal healthy controls.

The inventor gathers standard compliant blood sample with Standard operation procedure SOP (SOP), the demography data that systematic collection is complete, clinical data etc., and having adopted Affymetrix6.0 chip to carry out full genome scanning, TaqMan gene type carries out the detection of Single locus etc.

The experimental technique of research mainly comprises following components specifically:

1. the selection of research sample

(1) clinical definite is primary lung cancer;

(2) with the contrasting of case age, gender matched;

This research adopts 9934 routine standard compliant samples to study altogether.

2. phenol-chloroform method extracts peripheral blood genomic dna, according to a conventional method operation.Conventionally can obtain 20-50ng/ μ lDNA, purity (ultraviolet 260OD and 280OD ratio) is at 1.6-2.0.

3.Affymetrix 6.0 chip detection

(1) get experimenter's complete genome DNA sample;

(2) on Affymetrix 6.0 chips (being purchased from U.S. Ang Fei company, lower same), carry out full genome scanning;

(3) detection relatively the difference difference of each genotype in primary lung cancer case and normal healthy controls.

4. the TaqMan gene type of single SNP

(1) get experimenter's DNA sample;

(2) design Auele Specific Primer and the specificity fluorescent probe pair of single SNP;

(3) carry out PCR reaction;

(4) detect and compare the distributional difference of different genotype in primary lung cancer case and normal healthy controls.

5. diagnostic reagent box preparation method

Affymetrix6.0 chip carries out full genome scanning and single SNP detects the rear genotype distribution frequency SNP that there were significant differences in primary lung cancer case and normal healthy controls that determines, as the index of primary pulmonary cancerous diagnose.The SNP that falls ill relevant with primary lung cancer finally filtering out forms auxiliary diagnostic box rs107401, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2285947, rs2895680, rs3969178, rs4693311, rs4766994, rs4894545, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, rs6043705 and rs11699642).Diagnostic reagent can comprise Auele Specific Primer and the specificity fluorescent probe pair of these SNP, and the reagent such as Taq enzyme, dNTP.

6. statistical analysis technique

The difference of using χ2-test,chi-square test (for classified variable) or student check (for continuous variable) comparison demographic characteristics etc. to distribute between research object group.With the additive model in logistic regression analysis, carry out association analysis.

In order further to study the comprehensive indication of these 63 SNP formations for the effect of early diagnosis, we have built a mathematical formula, consider positive and negative associated situation and relation intensity that each SNP falls ill with primary lung cancer.Specifically, we mark to three of each SNP kinds of genotype, wild homozygous=" 0 ", heterozygous=" 1 ", homozygous=" 2 " make a variation, regression coefficient under additive model while take single snp analysis is weight, and the situation that considers each SNP is determined a dangerous score value to each research research contrast.The computational methods of dangerous score value are as follows:Dangerous score value=, (, the scoring of-0.15 * rs9439527, ), +, (, the scoring of 0.33 * rs17728461, ), +, (, the scoring of 0.14 * rs2225588, ), +, (, the scoring of 0.25 * rs510321, ), +, (, the scoring of-0.12 * rs663414, ), +, (, the scoring of 0.14 * rs4669621, ), +, (, the scoring of 0.25 * rs6490294, ), +, (, the scoring of 0.13 * rs4894545, ), +, (, the scoring of 0.14 * rs6780858, ), +, (, the scoring of-0.16 * rs107401, ), +, (, the scoring of-0.16 * rs1663689, ), +, (, the scoring of 0.16 * rs4697406, ), +, (, the scoring of 0.18 * rs1014588, ), +, (, the scoring of-0.13 * rs2405271, ), +, (, the scoring of-0.16 * rs6490820, ), +, (, the scoring of-0.20 * rs9463490, ), +, (, the scoring of 0.27 * rs2788713, ), +, (, the scoring of 0.22 * rs855641, ), +, (, the scoring of-0.22 * rs3130975, ), +, (, the scoring of-0.16 * rs9397087, ), +, (, the scoring of 0.20 * rs622917, ), +, (, the scoring of 0.20 * rs12793370, ), +, (, the scoring of 0.14 * rs11155286, ), +, (, the scoring of 0.15 * rs2285947, ), +, (, the scoring of 0.21 * rs2395166, ), +, (, the scoring of 0.22 * rs741061, ), +, (, the scoring of-0.17 * rs17058190, ), +, (, the scoring of-0.17 * rs11144475, ), +, (, the scoring of-0.14 * rs12002185, ), +, (, the scoring of 0.16 * rs4749794, ), +, (, the scoring of-0.14 * rs16883398, ), +, (, the scoring of 0.13 * rs1726744, ), +, (, the scoring of-0.17 * rs2030378, ), +, (, the scoring of 0.19 * rs2895680, ), +, (, the scoring of 0.25 * rs4940849, ), +, (, the scoring of-0.22 * rs3969178, ), +, (, the scoring of 0.19 * rs6102795, ), +, (, the scoring of-0.15 * rs17075390, ), +, (, the scoring of 0.14 * rs9317873, ), +, (, the scoring of 0.13 * rs9556016, ), +, (, the scoring of 0.17 * rs4809957, ), +, (, the scoring of-0.24 * rs12050469, ), +, (, the scoring of 0.14 * rs16959825, ), +, (, the scoring of 0.26 * rs9373105, ), +, (, the scoring of 0.12 * rs948783, ), +, (, the scoring of-0.13 * rs11907465, ), +, (, the scoring of-0.22 * rs6043705, ), +, (, the scoring of 0.16 * rs2317683, ), +, (, 0.17 * rs11264579 scoring)+(scoring of 0.17 * rs636586)+(scoring of 0.14 * rs13000907)+(scoring of 0.17 * rs1544694)+(scoring of 0.25 * rs12992554)+(scoring of 0.18 * rs11699642)+(scoring of 0.17 * rs4493136)+(scoring of 0.18 * rs4766994)+(scoring of 0.26 * rs4344933)+(scoring of 0.18 * rs4488809)+(scoring of 0.28 * rs1039119)+(scoring of 0.15 * rs4693311)+(scoring of 0.16 * rs2736100)+(scoring of 0.28 * rs465498)+(scoring of 0.17 * rs10066153)), the danger obtaining divides in the 5408 routine samples that value coefficient and boundary value be applied directly to genome-wide association study.

Statistical analysis all completes (PLINK1.07) by special statistical analysis software.The horizontal P value of significance,statistical is made as 0.05, and all statistical test are two-tailed test.

Below further instruction of the present invention:

In above-mentioned 2331 routine qualified primary lung cancer cases and 3077 routine normal healthy controls, two groups of ages, sex equilibrium are comparable.We carry out full genome scanning by these two groups of crowds through Affymetrix6.0 chip and obtain correlated results.

According to Affymetrix6.0 chip detection, the inventor detects the SNP that genotype distribution frequency there are differences in " primary lung cancer case " group and " normal healthy controls " group and comprises: rs107401, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2283947, rs2893680, rs3969178, rs4693311, rs4766994, rs4894343, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, rs6043705 and rs11699642.

According to above-mentioned detected result, we by these 63 to primary lung cancer fall ill relevant SNP other 2283 routine primary lung cancer cases and with 2243 routine normal healthy controls of its age, gender matched in carried out the detection of single SNP, result is consistent with chip detection.

Single factor and logistic Regression Analysis result all show, these 63 SNP exist remarkable associated with the morbidity of primary lung cancer.

Further analyze the combination of these 63 SNP for the effect of primary pulmonary cancerous diagnose, find that its combination can be good at distinguishing case and contrasts.

According to above-mentioned experimental result, the inventor prepared a kind of can be for the test kit of primary lung cancer auxiliary diagnosis, comprise the Auele Specific Primer, specificity fluorescent probe of measuring above-mentioned SNP in experimenter's blood specimen DNA to other detection reagent.

Particularly, the combination of these 63 SNP, or the right dependent diagnostic test kit constituting of the Auele Specific Primer of these 63 SNP and specificity fluorescent probe contributes to the auxiliary diagnosis of primary lung cancer, for clinician quick and precisely grasps patient's morbid state and coincident with severity degree of condition, take in time the scheme of preventing and treating of more personalized to provide support.

Beneficial effect of the present invention:

SNP mark provided by the invention is as the superiority of the mark of primary lung cancer auxiliary judgment:

(1) SNP is a kind of novel gene biomarker, be different from traditional biological mark, stable, Wicresoft, be easy to detect, susceptibility and the specificity of medical diagnosis on disease will be improved greatly, the successful exploitation of such biomarker is by starting brand-new situation for the diagnosis of primary lung cancer and treatment, for the development of other diseases biomarker is offered reference.

(2) SNP test kit is a kind of system, comprehensive diagnostic kit, the auxiliary diagnosis that can be used for primary lung cancer, contribute to reflect patient's morbid state, for clinician quick and precisely grasps conditions of patients, takes the scheme of preventing and treating of more personalized to provide support in time.

(3) adopt tight checking and appraisement system, the inventor adopts full genome chip to scan to obtain the SNP spectrum of disease-related at the initial stage, and applies TaqMan methods of genotyping and verify in large sample; Above method and tactful application acceleration and guaranteed SNP biomarker and diagnostic kit application are clinically also the development supplying method of other diseases biomarker and the reference on strategy.

The present invention is by controlling the influence factor to disease progression such as age, sex, smoking, and research SNP, in the application prospect of primary lung cancer auxiliary diagnosis, sets forth SNP for the impact of primary lung cancer progress, discloses its diagnostic value.Therefore, the present invention has obtained primary lung cancer morbidity related SNP spectrum and Specific marker; By the development and application of SNP biomarker and diagnostic kit, can make the diagnosis of primary lung cancer more convenient and easy, for clinician quick and precisely grasps conditions of patients, for clinical therapeutic efficacy evaluation lays the foundation, and for finding that the new small molecule drug targets with potential therapeutic value offers help.

Accompanying drawing explanation

Fig. 1: the ROC curve that shows genome-wide association study case group and control group.

Show the ROC curve that primary lung cancer case group is reference to normal healthy controls group.

Embodiment

The collection of embodiment 1 sample and the arrangement of sample data

Contriver started to have collected in January, 2011 from tumor center of Nanjing Medical University a large amount of Patients With Primary Lung Cancer blood specimens 2005 month April, by the arrangement to sample data, contriver has therefrom selected 9934 examples to meet the full genome chip scanning of sample of following standard and the laboratory sample of single SNP TaqMan gene type:

1, the Patients With Primary Lung Cancer that histopathology is made a definite diagnosis;

2, with the normal healthy controls of case age, gender matched;

And system acquisition the situation such as the demography data of these samples and clinical data.

The full genome scanning of SNP in embodiment 2 peripheral blood DNAs

In above-mentioned qualified 2331 routine Patients With Primary Lung Cancers and 3077 routine normal healthy controls, two groups of ages, gender matched.These two groups of crowds are obtained to correlated results through Affymetrix6.0 chip detection.Concrete steps are:

1, to the white corpuscle being stored in 2ml cryopreservation tube, add haemolysis reagent, put upside down to mix and proceed to completely afterwards.

2, remove red corpuscle: with haemolysis reagent, 5ml centrifuge tube is mended to 4ml, put upside down and mix, centrifugal 10 minutes of 4000rpm, abandons supernatant.In precipitation, add 4ml haemolysis reagent, again put upside down and mix cleaning once, centrifugal 10 minutes of 4000rpm, abandons supernatant.

3, extracting DNA: add 1ml extract and (contain 122.5ml 0.2M sodium-chlor in every 300ml in precipitation, 14.4ml 0.5M ethylenediamine tetraacetic acid (EDTA), 15ml 10% sodium lauryl sulphate, 148.1ml distilled water, lower same) and 8 μ l Proteinase Ks, on oscillator, fully concussion mixes, and 37 ℃ of water-baths are spent the night.

4, remove protein: add the saturated phenol of 1ml and fully mix (have gentle hands is shaken 15 minutes), centrifugal 10 minutes of 4000rpm, gets supernatant and proceed in new 5ml centrifuge tube.In supernatant liquor, add equal-volume chloroform and primary isoamyl alcohol mixed solution (chloroform: primary isoamyl alcohol=24: 1, v/v, lower same), after fully mixing (hand 15 minutes), centrifugal 10 minutes of 4000rpm, gets supernatant (being divided into the centrifuge tube of two 1.5ml).

5, DNA precipitation: in supernatant liquor, add the sodium-acetate 60 μ l of 3M, then add and the isopyknic ice dehydrated alcohol of supernatant liquor, upper and lower jog, visible white flocculent precipitate, then with the centrifugal 10min of 12000rpm.

6, DNA washing: add ice dehydrated alcohol 1ml in precipitation, the centrifugal 10min of 12000rpm, abandons supernatant final vacuum and drain or be placed in clean dry environment evaporate to dryness.

7, measure concentration: conventionally can obtain 20-50ng/ μ l DNA, purity (ultraviolet 260OD and 280OD ratio) is at 1.6-2.0.

8, carry out full genome scanning;

9, data analysis and processing: the genotype distribution frequency SNP that there were significant differences finding in " primary lung cancer case " group and " normal healthy controls " group is enumerated out hereinbefore, the results are shown in Table 1.

Table 1 case group and control group whole-genome association result

The TaqMan gene type of embodiment 3 single SNP

Above-mentioned full genome scanning is found and the primary lung cancer relevant SNP that falls ill detects in other 2283 primary lung cancer cases and 2243 normal healthy controls, concrete steps are:

3, extracting DNA: add 1ml extract and 8 μ l Proteinase Ks in precipitation, on oscillator, fully concussion mixes, and 37 ℃ of water-baths are spent the night.

4, remove protein: add the saturated phenol of 1ml and fully mix (have gentle hands is shaken 15 minutes), centrifugal 10 minutes of 4000rpm, gets supernatant and proceed in new 5ml centrifuge tube.In supernatant liquor, add equal-volume chloroform and primary isoamyl alcohol mixed solution (chloroform: primary isoamyl alcohol=24: 1), after fully mixing (hand 15 minutes), centrifugal 10 minutes of 4000rpm, gets supernatant (being divided into the centrifuge tube of two 1.5ml).

8, carry out TaqMan gene type.63 positive associated SNP that full genome scanning is found design Auele Specific Primer and specificity fluorescent probe pair.Reaction system comprises mixture 0.25 μ l (20pmol/ μ l), 0.125 μ l TaqMan fluorescent probe (10pmol/ μ l contains corresponding two kinds of allelic fluorescent probes simultaneously) and the 1.25 μ l distilled waters of 2.5 μ l 2X TaqMan gene type Master Mix, the every pair of forward and reverse primer.Add 0.5 μ lDNA.What instrument used is ABI Prism 7900 quantitative real time PCR Instruments, and PCR reaction conditions is: within 95 ℃, 5 minutes, carry out 1 circulation → 95 ℃, 15 seconds, within 60 ℃, 1 minute, carry out 40 circulations.

9, genotype interpretation: adopt ABI Prism 7900 quantitative real time PCR Instruments to carry out.

10, data processing and analysis: utilize the difference of the three kinds of genotype distribution frequency in case combination control group of each SNP of addtive model comparison in logistic regression model, result is no longer listed with genome scanning is similar entirely.

Embodiment 4 utilizes risk assessment separating method further to analyze SNP and primary lung cancer morbidity

According to the above results, the inventor is by the comparison to 2 groups of samples (" primary lung cancer case group " and " normal healthy controls group ") genotype distribution frequency, select positive associated SNP, the single SNP regression coefficient in full genome scanning sample of take is weight, further try to achieve dangerous score value, draw ROC and assess susceptibility and the specificity of prediction, and then assess the judgement of these SNP to primary lung cancer morbidity.The Conjoint Analysis of 63 SNP marks is found, these 63 SNP separate normal healthy controls group and primary lung cancer case group with 72.47% AUC, and the sensitivity of best stagnation point is 64.41%, specific degree: 69.71% (Fig. 1).

Therefore, the inventor has proved employing rs107401, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2285947, rs2895680, rs3969178, rs4693311, rs4766994, rs4894545, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, the combination of rs6043705 and rs11699642 can be distinguished normal healthy controls and Patients With Primary Lung Cancer well.

Embodiment 5 is for the making of primary lung cancer auxiliary diagnosis SNP test kit

The making of SNP test kit and operating process are based on Affymetrix6.0 chip detection and TaqMan genotyping technique.Kit contains a collection of SNP Auele Specific Primer, and (primer sequence that comprises following primer: rs107401 is SEQ ID No:1 and SEQ ID No:2; The primer sequence of rs465498 is SEQ ID No:5 and SEQ ID No:6; The primer sequence of rs663414 is SEQ ID No:9 and SEQ ID No:10; The primer sequence of rs622917 is SEQ IDNo:13 and SEQ ID No:14; The primer sequence of rs636586 is SEQ ID No:17 and SEQ ID No:18; The primer sequence of rs741061 is SEQ ID No:21 and SEQ ID No:22; The primer sequence of rs855641 is SEQID No:25 and SEQ ID No:26; The primer sequence of rs948783 is SEQ ID No:29 and SEQ ID No:30; The primer sequence of rs1544694 is SEQ ID No:33 and SEQ ID No:34; The primer sequence of rs1663689 is SEQID No:37 and SEQ ID No:38; The primer sequence of rs2030378 is SEQ ID No:41 and SEQ ID No:42; The primer sequence of rs2225588 is SEQ ID No:45 and SEQ ID No:46; The primer sequence of rs2317683 is SEQID No:49 and SEQ ID No:50; The primer sequence of rs2395166 is SEQ ID No:53 and SEQ ID No:54; The primer sequence of rs2405271 is SEQ ID No:57 and SEQ ID No:58; The primer sequence of rs2788713 is SEQID No:61 and SEQ ID No:62; The primer sequence of rs3130975 is SEQ ID No:65 and SEQ ID No:66; The primer sequence of rs4344933 is SEQ ID No:69 and SEQ ID No:70; The primer sequence of rs4488809 is SEQ ID No:73 and SEQ ID No:74; The primer sequence of rs4493136 is SEQ ID No:77 and SEQ IDNo:78; The primer sequence of rs4669621 is SEQ ID No:81 and SEQ ID No:82; The primer sequence of rs4697406 is SEQ ID No:85 and SEQ ID No:86; The primer sequence of rs4749794 is SEQ ID No:89 and SEQ ID No:90; The primer sequence of rs4809957 is SEQ ID No:93 and SEQ ID No:94;The primer sequence of rs6102795 is SEQ ID No:97 and SEQ ID No:98; The primer sequence of rs6490820 is SEQ IDNo:101 and SEQ ID No:102; The primer sequence of rs9317873 is SEQ ID No:105 and SEQ IDNo:106; The primer sequence of rs9373105 is SEQ ID No:109 and SEQ ID No:110; The primer sequence of rs9397087 is SEQ ID No:113 and SEQ ID No:114; The primer sequence of rs9463490 is SEQ ID No:117 and SEQ ID No:118; The primer sequence of rs10066153 is SEQ ID No:121 and SEQ ID No:122; The primer sequence of rs11144475 is SEQ ID No:125 and SEQ ID No:126; The primer sequence of rs11155286 is SEQ ID No:129 and SEQ ID No:130; The primer sequence of rs11264579 is SEQ ID No:133 and SEQ ID No:134; The primer sequence of rs11907465 is SEQ ID No:137 and SEQ ID No:138; The primer sequence of rs12050469 is SEQ ID No:141 and SEQ ID No:142; The primer sequence of rs12992554 is SEQ ID No:145 and SEQ ID No:146; The primer sequence of rs13000907 is SEQ ID No:149 and SEQ ID No:150; The primer sequence of rs16883398 is SEQ ID No:153 and SEQ ID No:154; The primer sequence of rs16959825 is SEQ ID No:157 and SEQ ID No:158; The primer sequence of rs17058190 is SEQ ID No:161 and SEQ ID No:162; The primer sequence of rs17075390 is SEQ ID No:165 and SEQ ID No:166; The primer sequence of rs17728461 is SEQ ID No:169 and SEQ ID No:170; The primer sequence of rs1014588 is SEQ ID No:173 and SEQ ID No:174; The primer sequence of rs1039119 is SEQ ID No:177 and SEQ ID No:178; The primer sequence of rs1726744 is SEQ ID No:181 and SEQ IDNo:182; The primer sequence of rs2285947 is SEQ ID No:185 and SEQ ID No:186;The primer sequence of rs2895680 is SEQ ID No:189 and SEQ ID No:190; The primer sequence of rs3969178 is SEQ ID No:193 and SEQ ID No:194; The primer sequence of rs4693311 is SEQ ID No:197 and SEQ ID No:198; The primer sequence of rs4766994 is SEQ ID No:201 and SEQ ID No:202; The primer sequence of rs4894545 is SEQ ID No:205 and SEQ ID No:206; The primer sequence of rs4940849 is SEQ ID No:209 and SEQ IDNo:210; The primer sequence of rs6490294 is SEQ ID No:213 and SEQ ID No:214; The primer sequence of rs6780858 is SEQ ID No:217 and SEQ ID No:218; The primer sequence of rs9439527 is SEQ ID No:221 and SEQ ID No:222; The primer sequence of rs9556016 is SEQ ID No:225 and SEQ ID No:226; The primer sequence of rs12002185 is SEQ ID No:229 and SEQ ID No:230; The primer sequence of rs12793370 is SEQ ID No:233 and SEQ ID No:234; The primer sequence of rs1549058 is SEQ ID No:237 and SEQID No:238; The primer sequence of rs2736100 is SEQ ID No:241 and SEQ ID No:242; The primer sequence of rs6043705 is SEQ ID No:245 and SEQ ID No:246; The primer sequence of rs11699642 is SEQ IDNo:249 and SEQ ID No:250), specificity fluorescent probe is to (fluorescence probe that comprises following fluorescence probe: rs107401 is SEQ ID No:3 and SEQ ID No:4 to sequence; The fluorescence probe of rs465498 is SEQ IDNo:7 and SEQ ID No:8 to sequence; The fluorescence probe of rs663414 is SEQ ID No:11 and SEQ ID No:12 to sequence; The fluorescence probe of rs622917 is SEQ ID No:15 and SEQ ID No:16 to sequence; The fluorescence probe of rs636586 is SEQ ID No:19 and SEQ ID No:20 to sequence; The fluorescence probe of rs741061 is SEQ ID No:23 and SEQ ID No:24 to sequence; The fluorescence probe of rs855641 is SEQ ID No:27 and SEQ ID No to sequence:28; The fluorescence probe of rs948783 is SEQ ID No:31 and SEQ ID No:32 to sequence; The fluorescence probe of rs1544694 is SEQ ID No:35 and SEQ ID No:36 to sequence; The fluorescence probe of rs1663689 is SEQ ID No:39 and SEQ ID No:40 to sequence; The fluorescence probe of rs2030378 is SEQ ID No:43 and SEQ ID No:44 to sequence; The fluorescence probe of rs2225588 is SEQ ID No:47 and SEQ ID No:48 to sequence; The fluorescence probe of rs2317683 is SEQ ID No:51 and SEQ ID No:52 to sequence; The fluorescence probe of rs2395166 is SEQ ID No:55 and SEQ ID No:56 to sequence; The fluorescence probe of rs2405271 is SEQ ID No:59 and SEQ ID No:60 to sequence; The fluorescence probe of rs2788713 is SEQ ID No:63 and SEQ ID No:64 to sequence; The fluorescence probe of rs3130975 is SEQ ID No:67 and SEQ ID No:68 to sequence; The fluorescence probe of rs4344933 is SEQ ID No:71 and SEQ ID No:72 to sequence; The fluorescence probe of rs4488809 is SEQ ID No:75 and SEQ ID No:76 to sequence; The fluorescence probe of rs4493136 is SEQ ID No:79 and SEQ ID No:80 to sequence; The fluorescence probe of rs4669621 is SEQ ID No:83 and SEQ ID No:84 to sequence; The fluorescence probe of rs4697406 is SEQ IDNo:87 and SEQ ID No:88 to sequence; The fluorescence probe of rs4749794 is SEQ ID No:91 and SEQ IDNo:92 to sequence; The fluorescence probe of rs4809957 is SEQ ID No:95 and SEQ ID No:96 to sequence; The fluorescence probe of rs6102795 is SEQ ID No:99 and SEQ ID No:100 to sequence; The fluorescence probe of rs6490820 is SEQ ID No:103 and SEQ ID No:104 to sequence; The fluorescence probe of rs9317873 is SEQ ID No:107 and SEQ ID No:108 to sequence; The fluorescence probe of rs9373105 is SEQ ID No:111 and SEQ ID No:112 to sequence; The fluorescence probe of rs9397087 is SEQ ID No:115 and SEQ ID No:116 to sequence;The fluorescence probe of rs9463490 is SEQ ID No:119 and SEQ ID No:120 to sequence; The fluorescence probe of rs10066153 is SEQID No:123 and SEQ ID No:124 to sequence; The fluorescence probe of rs11144475 is SEQ ID No:127 and SEQID No:128 to sequence; The fluorescence probe of rs11155286 is SEQ ID No:131 and SEQ ID No:132 to sequence; The fluorescence probe of rs11264579 is SEQ ID No:135 and SEQ ID No:136 to sequence; The fluorescence probe of rs11907465 is SEQ ID No:139 and SEQ ID No:140 to sequence; The fluorescence probe of rs12050469 is SEQID No:143 and SEQ ID No:144 to sequence; The fluorescence probe of rs12992554 is SEQ ID No:147 and SEQID No:148 to sequence; The fluorescence probe of rs13000907 is SEQ ID No:151 and SEQ ID No:152 to sequence; The fluorescence probe of rs16883398 is SEQ ID No:155 and SEQ ID No:156 to sequence; The fluorescence probe of rs16959825 is SEQ ID No:159 and SEQ ID No:160 to sequence; The fluorescence probe of rs17058190 is SEQID No:163 and SEQ ID No:164 to sequence; The fluorescence probe of rs17075390 is SEQ ID No:167 and SEQID No:168 to sequence; The fluorescence probe of rs17728461 is SEQ ID No:171 and SEQ ID No:172 to sequence; The fluorescence probe of rs1014588 is SEQ ID No:175 and SEQ ID No:176 to sequence; The fluorescence probe of rs1039119 is SEQ ID No:179 and SEQ ID No:180 to sequence; The fluorescence probe of rs1726744 is SEQ IDNo:183 and SEQ ID No:184 to sequence; The fluorescence probe of rs2285947 is SEQ ID No:187 and SEQ IDNo:188 to sequence; The fluorescence probe of rs2895680 is SEQ ID No:191 and SEQ ID No:192 to sequence; The fluorescence probe of rs3969178 is SEQ ID No:195 and SEQ ID No:196 to sequence; The fluorescence probe of rs4693311 is SEQ ID No:199 and SEQ ID No:200 to sequence; The fluorescence probe of rs4766994 is SEQ ID No to sequence:203 and SEQ ID No:204; The fluorescence probe of rs4894545 is SEQ ID No:207 and SEQ ID No:208 to sequence; The fluorescence probe of rs4940849 is SEQ ID No:211 and SEQ ID No:212 to sequence; The fluorescence probe of rs6490294 is SEQ ID No:215 and SEQ ID No:216 to sequence; The fluorescence probe of rs6780858 is SEQ IDNo:219 and SEQ ID No:220 to sequence; The fluorescence probe of rs9439527 is SEQ ID No:223 and SEQ IDNo:224 to sequence; The fluorescence probe of rs9556016 is SEQ ID No:227 and SEQ ID No:228 to sequence; The fluorescence probe of rs12002185 is SEQ ID No:231 and SEQ ID No:232 to sequence; The fluorescence probe of rs12793370 is SEQ ID No:235 and SEQ ID No:236 to sequence; The fluorescence probe of rs1549058 is SEQ ID No:239 and SEQ ID No:240 to sequence; The fluorescence probe of rs2736100 is SEQ ID No:243 and SEQ ID No:244 to sequence; The fluorescence probe of rs6043705 is SEQ ID No:247 and SEQ ID No:248 to sequence; The fluorescence probe of rs11699642 is SEQ ID No:251 and SEQ ID No:252 to sequence), can also there is the required common agents of corresponding round pcr, as: dNTPs, MgCl2, distilled water, fluorescence probe, Taq enzyme etc., these common agents are all well known to those skilled in the art, can also have in addition standard items and contrast (as determined genotypic standard items and blank etc.).The value of this test kit is only to need peripheral blood and does not need other tissue sample, by simplifying most with special primer and fluorescent probe detecting SNP, by SNP, compose auxiliary judgment primary lung cancer again, not only stable, easy to detect, and accurately, greatly improve susceptibility and the specificity of medical diagnosis on disease, therefore this test kit is dropped into practice, can help to instruct diagnosis and more effective individualized treatment.

Claims

1. the Auele Specific Primer of the SNP mark that detection is relevant to primary lung cancer auxiliary diagnosis and the right composition of fluorescent probe, is characterized in that, by following Auele Specific Primer and fluorescent probe to forming,

Its Auele Specific Primer is:

The primer sequence of rs107401 is SEQ ID No:1 and SEQ ID No:2;

The primer sequence of rs465498 is SEQ ID No:5 and SEQ ID No:6;

The primer sequence of rs663414 is SEQ ID No:9 and SEQ ID No:10;

The primer sequence of rs622917 is SEQ ID No:13 and SEQ ID No:14;

The primer sequence of rs636586 is SEQ ID No:17 and SEQ ID No:18;

The primer sequence of rs741061 is SEQ ID No:21 and SEQ ID No:22;

The primer sequence of rs855641 is SEQ ID No:25 and SEQ ID No:26;

The primer sequence of rs948783 is SEQ ID No:29 and SEQ ID No:30;

The primer sequence of rs1544694 is SEQ ID No:33 and SEQ ID No:34;

The primer sequence of rs1663689 is SEQ ID No:37 and SEQ ID No:38;

The primer sequence of rs2030378 is SEQ ID No:41 and SEQ ID No:42;

The primer sequence of rs2225588 is SEQ ID No:45 and SEQ ID No:46;

The primer sequence of rs2317683 is SEQ ID No:49 and SEQ ID No:50;

The primer sequence of rs2395166 is SEQ ID No:53 and SEQ ID No:54;

The primer sequence of rs2405271 is SEQ ID No:57 and SEQ ID No:58;

The primer sequence of rs2788713 is SEQ ID No:61 and SEQ ID No:62;

The primer sequence of rs3130975 is SEQ ID No:65 and SEQ ID No:66;

The primer sequence of rs4344933 is SEQ ID No:69 and SEQ ID No:70;

The primer sequence of rs4488809 is SEQ ID No:73 and SEQ ID No:74;

The primer sequence of rs4493136 is SEQ ID No:77 and SEQ ID No:78;

The primer sequence of rs4669621 is SEQ ID No:81 and SEQ ID No:82;

The primer sequence of rs4697406 is SEQ ID No:85 and SEQ ID No:86;

The primer sequence of rs4749794 is SEQ ID No:89 and SEQ ID No:90;

The primer sequence of rs4809957 is SEQ ID No:93 and SEQ ID No:94;

The primer sequence of rs6102795 is SEQ ID No:97 and SEQ ID No:98;

The primer sequence of rs6490820 is SEQ ID No:101 and SEQ ID No:102;

The primer sequence of rs9317873 is SEQ ID No:105 and SEQ ID No:106;

The primer sequence of rs9373105 is SEQ ID No:109 and SEQ ID No:110;

The primer sequence of rs9397087 is SEQ ID No:113 and SEQ ID No:114;

The primer sequence of rs9463490 is SEQ ID No:117 and SEQ ID No:118;

The primer sequence of rs10066153 is SEQ ID No:121 and SEQ ID No:122;

The primer sequence of rs11144475 is SEQ ID No:125 and SEQ ID No:126;

The primer sequence of rs11155286 is SEQ ID No:129 and SEQ ID No:130;

The primer sequence of rs11264579 is SEQ ID No:133 and SEQ ID No:134;

The primer sequence of rs11907465 is SEQ ID No:137 and SEQ ID No:138;

The primer sequence of rs12050469 is SEQ ID No:141 and SEQ ID No:142;

The primer sequence of rs12992554 is SEQ ID No:145 and SEQ ID No:146;

The primer sequence of rs13000907 is SEQ ID No:149 and SEQ ID No:150;

The primer sequence of rs16883398 is SEQ ID No:153 and SEQ ID No:154;

The primer sequence of rs16959825 is SEQ ID No:157 and SEQ ID No:158;

The primer sequence of rs17058190 is SEQ ID No:161 and SEQ ID No:162;

The primer sequence of rs17075390 is SEQ ID No:165 and SEQ ID No:166;

The primer sequence of rs17728461 is SEQ ID No:169 and SEQ ID No:170;

The primer sequence of rs1014588 is SEQ ID No:173 and SEQ ID No:174;

The primer sequence of rs1039119 is SEQ ID No:177 and SEQ ID No:178;

The primer sequence of rs1726744 is SEQ ID No:181 and SEQ ID No:182;

The primer sequence of rs2285947 is SEQ ID No:185 and SEQ ID No:186;

The primer sequence of rs2895680 is SEQ ID No:189 and SEQ ID No:190;

The primer sequence of rs3969178 is SEQ ID No:193 and SEQ ID No:194;

The primer sequence of rs4693311 is SEQ ID No:197 and SEQ ID No:198;

The primer sequence of rs4766994 is SEQ ID No:201 and SEQ ID No:202;

The primer sequence of rs4894545 is SEQ ID No:205 and SEQ ID No:206;

The primer sequence of rs4940849 is SEQ ID No:209 and SEQ ID No:210;

The primer sequence of rs6490294 is SEQ ID No:213 and SEQ ID No:214;

The primer sequence of rs6780858 is SEQ ID No:217 and SEQ ID No:218;

The primer sequence of rs9439527 is SEQ ID No:221 and SEQ ID No:222;

The primer sequence of rs9556016 is SEQ ID No:225 and SEQ ID No:226;

The primer sequence of rs12002185 is SEQ ID No:229 and SEQ ID No:230;

The primer sequence of rs12793370 is SEQ ID No:233 and SEQ ID No:234;

The primer sequence of rs1549058 is SEQ ID No:237 and SEQ ID No:238;

The primer sequence of rs2736100 is SEQ ID No:241 and SEQ ID No:242;

The primer sequence of rs6043705 is SEQ ID No:245 and SEQ ID No:246;

The primer sequence of rs11699642 is SEQ ID No:249 and SEQ ID No:250;

Its fluorescent probe is to being:

The fluorescent probe of rs107401 is SEQ ID No:3 and SEQ ID No:4 to sequence;

The fluorescent probe of rs465498 is SEQ ID No:7 and SEQ ID No:8 to sequence;

The fluorescent probe of rs663414 is SEQ ID No:11 and SEQ ID No:12 to sequence;

The fluorescent probe of rs622917 is SEQ ID No:15 and SEQ ID No:16 to sequence;

The fluorescent probe of rs636586 is SEQ ID No:19 and SEQ ID No:20 to sequence;

The fluorescent probe of rs741061 is SEQ ID No:23 and SEQ ID No:24 to sequence;

The fluorescent probe of rs855641 is SEQ ID No:27 and SEQ ID No:28 to sequence;

The fluorescent probe of rs948783 is SEQ ID No:31 and SEQ ID No:32 to sequence;

The fluorescent probe of rs1544694 is SEQ ID No:35 and SEQ ID No:36 to sequence;

The fluorescent probe of rs1663689 is SEQ ID No:39 and SEQ ID No:40 to sequence;

The fluorescent probe of rs2030378 is SEQ ID No:43 and SEQ ID No:44 to sequence;

The fluorescent probe of rs2225588 is SEQ ID No:47 and SEQ ID No:48 to sequence;

The fluorescent probe of rs2317683 is SEQ ID No:51 and SEQ ID No:52 to sequence;

The fluorescent probe of rs2395166 is SEQ ID No:55 and SEQ ID No:56 to sequence;

The fluorescent probe of rs2405271 is SEQ ID No:59 and SEQ ID No:60 to sequence;

The fluorescent probe of rs2788713 is SEQ ID No:63 and SEQ ID No:64 to sequence;

The fluorescent probe of rs3130975 is SEQ ID No:67 and SEQ ID No:68 to sequence;

The fluorescent probe of rs4344933 is SEQ ID No:71 and SEQ ID No:72 to sequence;

The fluorescent probe of rs4488809 is SEQ ID No:75 and SEQ ID No:76 to sequence;

The fluorescent probe of rs4493136 is SEQ ID No:79 and SEQ ID No:80 to sequence;

The fluorescent probe of rs4669621 is SEQ ID No:83 and SEQ ID No:84 to sequence;

The fluorescent probe of rs4697406 is SEQ ID No:87 and SEQ ID No:88 to sequence;

The fluorescent probe of rs4749794 is SEQ ID No:91 and SEQ ID No:92 to sequence;

The fluorescent probe of rs4809957 is SEQ ID No:95 and SEQ ID No:96 to sequence;

The fluorescent probe of rs6102795 is SEQ ID No:99 and SEQ ID No:100 to sequence;

The fluorescent probe of rs6490820 is SEQ ID No:103 and SEQ ID No:104 to sequence;

The fluorescent probe of rs9317873 is SEQ ID No:107 and SEQ ID No:108 to sequence;

The fluorescent probe of rs9373105 is SEQ ID No:111 and SEQ ID No:112 to sequence;

The fluorescent probe of rs9397087 is SEQ ID No:115 and SEQ ID No:116 to sequence;

The fluorescent probe of rs9463490 is SEQ ID No:119 and SEQ ID No:120 to sequence;

The fluorescent probe of rs10066153 is SEQ ID No:123 and SEQ ID No:124 to sequence;

The fluorescent probe of rs11144475 is SEQ ID No:127 and SEQ ID No:128 to sequence;

The fluorescent probe of rs11155286 is SEQ ID No:131 and SEQ ID No:132 to sequence;

The fluorescent probe of rs11264579 is SEQ ID No:135 and SEQ ID No:136 to sequence;

The fluorescent probe of rs11907465 is SEQ ID No:139 and SEQ ID No:140 to sequence;

The fluorescent probe of rs12050469 is SEQ ID No:143 and SEQ ID No:144 to sequence;

The fluorescent probe of rs12992554 is SEQ ID No:147 and SEQ ID No:148 to sequence;

The fluorescent probe of rs13000907 is SEQ ID No:151 and SEQ ID No:152 to sequence;

The fluorescent probe of rs16883398 is SEQ ID No:155 and SEQ ID No:156 to sequence;

The fluorescent probe of rs16959825 is SEQ ID No:159 and SEQ ID No:160 to sequence;

The fluorescent probe of rs17058190 is SEQ ID No:163 and SEQ ID No:164 to sequence;

The fluorescent probe of rs17075390 is SEQ ID No:167 and SEQ ID No:168 to sequence;

The fluorescent probe of rs17728461 is SEQ ID No:171 and SEQ ID No:172 to sequence;

The fluorescent probe of rs1014588 is SEQ ID No:175 and SEQ ID No:176 to sequence;

The fluorescent probe of rs1039119 is SEQ ID No:179 and SEQ ID No:180 to sequence;

The fluorescent probe of rs1726744 is SEQ ID No:183 and SEQ ID No:184 to sequence;

The fluorescent probe of rs2285947 is SEQ ID No:187 and SEQ ID No:188 to sequence;

The fluorescent probe of rs2895680 is SEQ ID No:191 and SEQ ID No:192 to sequence;

The fluorescent probe of rs3969178 is SEQ ID No:195 and SEQ ID No:196 to sequence;

The fluorescent probe of rs4693311 is SEQ ID No:199 and SEQ ID No:200 to sequence;

The fluorescent probe of rs4766994 is SEQ ID No:203 and SEQ ID No:204 to sequence;

The fluorescent probe of rs4894545 is SEQ ID No:207 and SEQ ID No:208 to sequence;

The fluorescent probe of rs4940849 is SEQ ID No:211 and SEQ ID No:212 to sequence;

The fluorescent probe of rs6490294 is SEQ ID No:215 and SEQ ID No:216 to sequence;

The fluorescent probe of rs6780858 is SEQ ID No:219 and SEQ ID No:220 to sequence;

The fluorescent probe of rs9439527 is SEQ ID No:223 and SEQ ID No:224 to sequence;

The fluorescent probe of rs9556016 is SEQ ID No:227 and SEQ ID No:228 to sequence;

The fluorescent probe of rs12002185 is SEQ ID No:231 and SEQ ID No:232 to sequence;

The fluorescent probe of rs12793370 is SEQ ID No:235 and SEQ ID No:236 to sequence;

The fluorescent probe of rs1549058 is SEQ ID No:239 and SEQ ID No:240 to sequence;

The fluorescent probe of rs2736100 is SEQ ID No:243 and SEQ ID No:244 to sequence;

The fluorescent probe of rs6043705 is SEQ ID No:247 and SEQ ID No:248 to sequence;

The fluorescent probe of rs11699642 is SEQ ID No:251 and SEQ ID No:252 to sequence.

2. the application of composition claimed in claim 1 in preparing primary lung cancer auxiliary diagnostic box.

3. a primary lung cancer auxiliary diagnostic box, is characterized in that this test kit is for detection of rs107401 in peripheral blood DNA, rs465498, rs663414, rs622917, rs636586, rs741061, rs855641, rs948783, rs1544694, rs1663689, rs2030378, rs2225588, rs2317683, rs2395166, rs2405271, rs2788713, rs3130975, rs4344933, rs4488809, rs4493136, rs4669621, rs4697406, rs4749794, rs4809957, rs6102795, rs6490820, rs9317873, rs9373105, rs9397087, rs9463490, rs10066153, rs11144475, rs11155286, rs11264579, rs11907465, rs12050469, rs12992554, rs13000907, rs16883398, rs16959825, rs17058190, rs17075390, rs17728461, rs1014588, rs1039119, rs1726744, rs2285947, rs2895680, rs3969178, rs4693311, rs4766994, rs4894545, rs4940849, rs6490294, rs6780858, rs9439527, rs9556016, rs12002185, rs12793370, rs1549058, rs2736100, rs6043705 and rs11699642, this test kit contains composition claimed in claim 1.

4. test kit according to claim 3, is characterized in that this test kit also comprises the reagent that round pcr is conventional.