CN102533622A - Actinobacillus succinogenes capable of producing succinic acid - Google Patents
Actinobacillus succinogenes capable of producing succinic acid Download PDFInfo
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- CN102533622A CN102533622A CN2012100565686A CN201210056568A CN102533622A CN 102533622 A CN102533622 A CN 102533622A CN 2012100565686 A CN2012100565686 A CN 2012100565686A CN 201210056568 A CN201210056568 A CN 201210056568A CN 102533622 A CN102533622 A CN 102533622A
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- succsinic acid
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- actinobacillus succinogenes
- succinic acid
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- 241000948980 Actinobacillus succinogenes Species 0.000 title claims abstract description 9
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 title abstract description 12
- 239000001384 succinic acid Substances 0.000 title abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 13
- 239000002253 acid Substances 0.000 claims description 62
- 241000894006 Bacteria Species 0.000 claims description 30
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- 239000008103 glucose Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 19
- 238000012216 screening Methods 0.000 abstract description 13
- 238000004128 high performance liquid chromatography Methods 0.000 abstract description 8
- 241000606750 Actinobacillus Species 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 238000004321 preservation Methods 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 2
- NAOLWIGVYRIGTP-UHFFFAOYSA-N 1,3,5-trihydroxyanthracene-9,10-dione Chemical compound C1=CC(O)=C2C(=O)C3=CC(O)=CC(O)=C3C(=O)C2=C1 NAOLWIGVYRIGTP-UHFFFAOYSA-N 0.000 abstract 1
- 239000011148 porous material Substances 0.000 abstract 1
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- 210000001938 protoplast Anatomy 0.000 abstract 1
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- 230000008521 reorganization Effects 0.000 description 18
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- 238000005516 engineering process Methods 0.000 description 6
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- 239000000047 product Substances 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 5
- 235000008504 concentrate Nutrition 0.000 description 5
- 238000012262 fermentative production Methods 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- JGFYQVQAXANWJU-UHFFFAOYSA-M sodium fluoroacetate Chemical compound [Na+].[O-]C(=O)CF JGFYQVQAXANWJU-UHFFFAOYSA-M 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 3
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
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- 108090000623 proteins and genes Proteins 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 241000722955 Anaerobiospirillum Species 0.000 description 2
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241000029538 [Mannheimia] succiniciproducens Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
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- 229940074404 sodium succinate Drugs 0.000 description 2
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 241000722954 Anaerobiospirillum succiniciproducens Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- -1 CCTCC NO:M2012036 Substances 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- 108010036781 Fumarate Hydratase Proteins 0.000 description 1
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 241001293418 Mannheimia haemolytica Species 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
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- 244000138286 Sorghum saccharatum Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
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- 229940116298 l- malic acid Drugs 0.000 description 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 1
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- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses actinobacillus succionogenes capable of producing succinic acid with high yield and a method for screening and producing the succinic acid by a fermentation method. The actinobacillus succinogenes takes CGMCC1593 as the original strain and is obtained by blending a plurality of turns of protoplast in a progressive way through a method of '96-pore plate culture, HPLC concentration detection and then anaerobic bottle re-screening'. The actinobacillus succinogenes has already been preserved on February 26, 2012 in China Center for Type Culture Collection with the preservation number CCTCCNO: M2012036. The actinobacillus succinogenes adopts fed-batch culture in a 5 to 15 L fermentation tank, 95.6 g/L succinic acid is produced in 48 hours, the production intensity is 1.99 g/(L.h), and the saccharic acid transformation rate is 0.71 g/g. Compared with other bacterial strains at home and abroad, the actinobacillus succinogenes has the advantages of high yield and lower production cost.
Description
Technical field
The present invention relates to the seed selection of the microorganism strains (F3-ZK) of a strain anaerobic condition bottom fermentation high yield succsinic acid, and fermentation is produced the succsinic acid process, the genus biological technical field.
Background technology
Succsinic acid, the formal name used at school Succinic Acid is because of gaining the name early than finding in the amber.As a kind of important C4 hardware and software platform compound, be widely used in medicine, food, degradable plastics and chemical industry.Succsinic acid mainly is to obtain through the MALEIC ANHYDRIDE MIN 99.5 hydrolysis that derives from petrochemical material.Because the negative impact that the non-renewable and petrochemical industry of petrochemical industry resource produces environment, the fermentative Production succsinic acid has become the focus of Recent study as a kind of emerging friendly process.
The fermentative Production succsinic acid has the characteristics of low-carbon (LC), clean environment firendly, meets the requirement of Sustainable development.Wherein breeding high-yield succsinic acid bacterial strain is to realize the industrialized crucial ring of fermentative Production succsinic acid.At present, the microorganism strains of having reported the fermentative Production succsinic acid has: produce the succsinic acid pleuropneumoniae (
Actinobacillus succinogenes), Anaerobiospirillum succinoproducens (
Anaerobiospirillum succiniciproducens), produce the succsinic acid mannheimia haemolytica (
Mannheimia Succiniciproducens), intestinal bacteria (
E.coli) and Corynebacterium glutamicum (
Corynebacterium glutanicum).The comparatively successful strictly anaerobic spirillum of early stage research
Anaerobiospirillum succiniciproducensATCC 29305, and it produces succsinic acid can reach 43g/L, and productive rate 91% (US 5,143, and 833; US 5,143,834 with US 5,168,055); The facultative anaerobe of University of Michigan and Michigan Biotech Inst's screening
Bacterium130Z (
Actinobacillus succinogenesATCC 55618), produce succsinic acid content and can reach 70g/L, productive rate 80% (US 5,04, and 004,1996; US 5,723, and 322,1998), its highest research level fermentation 78h produces acid and reaches 106.8 g/L, to glucose productive rate 83% (US 5573931,1996); Study group of Korean Institute of Science and Technology screening Man succsinic acid bacillus
Mannheimia succiniciproducens, the high yield succsinic acid level of report is 52.4 g/L, production intensity 1.8 g/Lh, (S. J. Lee et al. Appl Envi Microbiol, 2006).The study group such as Clark DP of the M. I. Donnelly of U.S. Argonne National Laboratory etc., southern Illinois university, the research and establishment bacillus coli gene engineering bacterias such as Mark A. Eiteman study group of University of Georgia, wherein
E.coliAFP111/pTrc99A-pyc highest level is produced sour 99.2 g/L (Vemuri G N et al. J Ind Microbiol Biotechnol, 2002).But above-mentioned have the bacterial strain of prospect of production to receive patent protection, is difficult for obtaining.
Chinese patent CN 1884484A has announced a strain
Actinobacillus succinogenesNJ113 is numbered CGMCC NO.171, and it produces succsinic acid can reach 38.5 g/L; Chinese patent CN1884484A has announced that a plant height produces the Corynebacterium glutamicum CGMCC NO.3991 of succsinic acid, the high yield succsinic acid 35.8g/L of its fermented liquid.This research group screens the wild strain that succsinic acid is produced in a strain early stage from the cud of ox, confirm as through strain identification and produce succsinic acid pleuropneumoniae CGMCC 1593 (Chinese patent ZL 200610038113.6); Through NTG mutagenic and breeding Tenate resistant mutant strain; Obtain the mutagenic strain SF-9 (industrial microorganism that output improves; 2007; 37 (2) 1-7), its 5L fermentation cylinder for fermentation 36h can accumulate succsinic acid 40.5g/L, and the seed selection of applying gene group shuffling technology (is F to anti-sodium, Tenate resistance reorganization bacterium CGMCC 2653
3-10, Chinese patent ZL 200810146688.9), this bacterial strain fed-batch fermentation 48h in the 5L fermentor tank produces succsinic acid 53.96g/L.Reported employing genome shuffling technology seed selection acid resistance succsinic acid pleuropneumoniae F in 2009
3-21, its fed-batch fermentation 72h in the 5L fermentor tank produces succsinic acid and reaches 67.4g/L (microbiology circular, 2009,36 (11): 1676-1681).But the product succsinic acid level majority of these bacterial strains does not reach 70g/L, and production intensity is below 1.3 g/ (Lh), and the cost that is applied to suitability for industrialized production is relatively still higher.
The present invention is directed to the problems referred to above, on the basis of previous work, adopt the genome shuffling technology, through improving screening method, seed selection is to the succsinic acid pleuropneumoniae F3-ZK of high yield succsinic acid, and its acid yield is 70-95.6 g/L.Than starting strain F
3-21 produce acid improves 41.8%, and production intensity improves 111.7%, and glucose acid invert ratio improves 9.2%.The advantage that this bacterial strain has high yield, reduces production costs.
Summary of the invention
The purpose of this invention is to provide a strain and produce the succsinic acid pleuropneumoniae of succsinic acid, its acid yield is 70-95.6 g/L.
Technical scheme of the present invention: the succsinic acid pleuropneumoniae of plant height product succsinic acid (
Actinobacillus succinogenes) F3-ZK, be preserved in Chinese typical culture collection center, preserving number CCTCC NO:M2012036.
The application of said CCTCC NO:M2012036 bacterial strain, this bacterium are in the 5-15L fermentor tank, and fed-batch fermentation 36-48 h produces succsinic acid 70-95.6 g/L, production intensity 1.70-1.99 g/ (Lh), glucose acid invert ratio 0.70-0.83 g/g.
One plant height produces the succsinic acid pleuropneumoniae of succsinic acid
Actinobacillus succinogenesF3-ZK is preserved in Chinese typical culture collection center the sixth of the twelve Earthly Branches, preserving number CCTCC NO:M2012036.Obtain through following seed selection: it is starting strain that CGMCC1593 changes the bacterial strain F3-21 that technology obtains through genome; Through trypaflavine, ultraviolet ray, ultraviolet ray-ethyl sulfate and nitrosoguanidine mutagenesis; Adopt the method for " 96 orifice plate cultivation-HPLC concentrate detection-anaerobism bottle and sieve again "; Obtain further to carry out the bacterium storehouse of setting out of genome reorganization, the fusion of going forward one by one of the protoplastis through three-wheel again obtains reorganizing bacterium
A. succinogenesF3-ZK.
Below be the detailed description of the inventive method:
Improved " 96 orifice plate cultivation-HPLC concentrate detection-anaerobism bottle and sieve again " screening method:
Trypaflavine, ultraviolet ray, ultraviolet ray-ethyl sulfate and nitrosoguanidine carry out mutagenic treatment to bacterial strain F3-21, and treatment solution is coated the selectivity flat board, and cultivate 3-5 d in 37 ℃ of anaerobism.The single bacterium colony that grows on the selectivity flat board inserts in the 96 porocyte culture plate fermention mediums with toothpick, places the anaerobism glove box at 100%CO
2Cultivate 24-72 h under the condition.Adopting concentrated detection method that HPLC is carried out in 96 holes detects: the first step: the coding rule of 96 orifice plate mesopores: earlier by 96 orifice plate batch fermentation numbering I, II, III, IV, V etc.; Then in every 96 orifice plate, by row serial number 1,2,3 ... 12 with listed sequence numbering A, B, C ... H; Second step: with every row such as 1-A, 1-B ... Fermented liquid equal-volume in the 1-H hole mixes, and carries out HPLC after the sample preparation and detects; The 3rd step: the single hole fermented liquid in the detected result the highest a group (supposing the 2nd group) is carried out next round detect, as to 2-A, 2-B ... Fermented liquid in the 2-H hole detects with HPLC respectively, measures quantity through reducing, and reaches the purpose that concentrates screening.Every Tissue Culture Plate is selected the 1-2 strain and is produced the highest bacterial strain of succsinic acid content, is inoculated into and carries out multiple sieve in the anaerobism bottle, produces the bacterium storehouse of setting out of the high preceding 5-15 strain bacterium constitutive gene group reorganization of acid.
Selecting flat board is in the TSB plate culture medium; Add 0.3-0.5 mol/L sodium succinate, 10-100 g/L Sodium.alpha.-ketopropionate, 1-20 g/L Tenate and 1-5 times of fermented liquid of dilution respectively; Process the flat board of corresponding sodium succinate; The flat board of Sodium.alpha.-ketopropionate, the flat board of Tenate and the flat board of fermented liquid.
Fermention medium press document microbiology circular (2009,36:1676-1681) in reported method.
Genome reorganization method:
Reorganization is set out and is transferred in another fresh culture behind the strain culturing 12-24 h in bacterium storehouse; It is centrifugal under the 6000-100000r/min rotating speed to get the cell that is in logarithmic phase basically; The PB damping fluid washs and processes the thalline suspension, in bacteria suspension, adds an amount of N,O-Diacetylmuramidase and obtains protoplastis in enzymolysis in 37 ℃ of water-baths.With being divided into two equal portions after the protoplastis suspension balanced mix that obtains, carry out ultraviolet (30 cm places irradiation 5-10 min under the 25 W uv lamps) and hot deactivation (55 ℃, 20-60 min) respectively; The inactivated bacteria suspension of getting equivalent mixes in centrifuge tube, and centrifugal back suspends with the PB damping fluid, adds 0.2-5mL PEG then and in 37 ℃ of water-baths, merges 5-20min; Centrifugal supernatant discarded; Suspend with the PB damping fluid, on the coating selectivity regenerated plate, be inverted in 37 ℃ of anaerobism incubators and cultivate 3-5 d.The fusant that grows on the regenerated plate is chosen into 96 porocyte culture plates,, carried out the screening of first round genome reorganization bacterium by " 96 orifice plate cultivation-HPLC concentrate detection-anaerobism bottle and sieve again " screening method.The 3-5 plant height that obtains is produced bacterium, as second take turns genome reorganization female parent, undertaken second taking turns, the prove genome reorganization for the third time by same flow process.
Organic acid product detection method is pressed Chinese patent ZL 200610038113.6 described methods.
Enzyme is lived and is analyzed the described method of document Arch Microbiol (1997,167: 332 – 342) of pressing.
Beneficial effect of the present invention: through improving screening method, seed selection is to the succsinic acid pleuropneumoniae F3-ZK of high yield succsinic acid, i.e. CCTCC NO:M2012036, and its acid yield is 70-95.6 g/L.Than starting strain F
3-21 produce acid improves 41.8%, and production intensity improves 111.7%, and glucose acid invert ratio improves 9.2%.The advantage that this bacterial strain has high yield, reduces production costs.
The biological material specimens preservation: the succsinic acid pleuropneumoniae of plant height product succsinic acid (
Actinobacillus succinogenes) F3-ZK, be preserved in Chinese typical culture collection center, be called for short CCTCC, address: Chinese Wuhan Wuhan University, preservation date on February 26th, 2012, deposit number CCTCC NO:M2012036.
Description of drawings
Fig. 1 F3-ZK reorganization bacterium 5 L stirred pot fed-batch fermentation curves.
Embodiment
Embodiment 1
Use trypaflavine, ultraviolet ray, ultraviolet ray-ethyl sulfate and nitrosoguanidine mutagenesis respectively
A. succinogenesF
3-21, obtain 1056 single bacterium colonies; These single bacterium obtain mutant strain that 11 strain succinic acid productions are higher than starting strain through " 96 orifice plate cultivations-HPLC concentrate detections-anaerobism bottle and sieve again ", and (III-9-H, IV-7-A, IV-7-C, V-12-B, VI-10-C, VII-11-H, VIII-10-H, IX-2-C, X-8-E, XI-8-B and XII-7-B), it on average shakes bottle product succsinic acid than F
3-21 have improved 13.9%.Be the bacterium storehouse of setting out with this 11 plant mutant bacterial strain.
Embodiment 2
Carry out the first round and merge as the reorganization bacterium storehouse of setting out with 11 strain mutagenicity high-yield bacterial strains; It is 0.25 mg/mL that 11 strain bacterium enzymatic hydrolysis conditions adopt enzyme concn; Enzymolysis time is 60 min, adopts the dead two deactivations of ultraviolet and thermic, and PEG merges back coating selectivity regeneration double-layer plate; Grow more than 360 single bacterium colony on the flat board altogether, these single bacterium colonies are chosen carried out flux screening in 96 orifice plates then.First round genome reorganization obtains 4 strain F
1For high yield reorganization bacterium (F
1-I-3-F, F
1-II-7-B, F
1-III-2-E and F
1-IV-9-D), it on average shakes bottle and produces succsinic acid than F
3-21 have improved 20.2%, with F
1Generation reorganization bacterium is that female parent carries out second and takes turns reorganization, and screening has obtained 4 strain F
2For high yield reorganization bacterium (F
2-I-5-B, F
2-II-5-H, F
2-III-6-D and F
2-III-10-F), it on average shakes bottle and produces succsinic acid than F
3-21 have improved 32.9%; Again with F
2Generation reorganization bacterium is that female parent carries out third round reorganization, and result's screening obtains 3 strain F
3For high yield reorganization bacterium (F
3-I-9-C, F
3-II-3-F, F
3-II-10-D), on average shake bottle and produce succsinic acid than F
3-21 have improved 45.7%, wherein F
3-II-3-F (name and be F3-ZK) is shaken bottle product succsinic acid and is reached 38.9 g/L, compares F
3-21 have improved 47.6%.
Embodiment 3
With the F3-ZK seed liquor, be equipped with in the 5L fermentor tank of 3.5L fermented liquid, in 37 ℃ by the access of 10% inoculum size; Anaerobically fermenting under mixing speed 200 r/min; Fermenting process is kept sugared concentration 10-30 g/L through replenishing glucose syrup, and controls pH with basic salt, keeps between the 5.8-6.4.Wherein, seed culture medium (every L): glucose 5 g, yeast extract paste 5 g, K
2HPO
43H
2O 1.0 g, NaH
2PO
42H
2O 1.0 g, pH 7.0.37 ℃ of anaerobism were cultivated 16 hours.Fermention medium (every L): sweet sorghum syrup (total reducing sugars) the 20-80 g that squeezes the juice, yeast extract paste 0-15 g, steeping water 20 g, MgCl
20.2 g, Na
2HPO
412 H
2O 1.5 g, NaH
2PO
42 H
2O 1.5 g, pH 6.5.37 ℃ of anaerobism were cultivated 48 hours.
Fermentation diagram such as Fig. 1.48 h produce succsinic acid 95.6 g/L.
Embodiment 4
Measure the activity of key enzyme in the pathways metabolism of reorganizing the bacterium F3-ZK and the bacterium F3-21 that sets out, result such as table 1.
The enzyme of key enzyme comparative result alive in table 1. F3-ZK and the F3-21 pathways metabolism
| Enzyme (U/mg) alive | F3-ZK | F3-21 |
| HK | 105 | 91 |
| 1, the 6-fructose-diphosphate aldolase | 722 | 723 |
| Glyceraldehyde 3-phosphate dehydro-genase | 408 | 412 |
| Hydratase, phosphoenolpyruvate | 2735 | 2689 |
| PEP carboxylation kinases | 1856 | 1250 |
| The L MALIC ACID desaturase | 926 | 928 |
| Fumarase | 3253 | 3427 |
| Fumaric reductase | 522 | 516 |
| Pyruvate kinase | 403 | 515 |
| Pyruvic acid-formic acid lyase | 251 | 259 |
| Phosphotransacetylase base enzyme | 320 | 341 |
| E.C. 2.7.2.1 | 1218 | 1432 |
| |
0 | 0 |
Claims (2)
- One plant height produce succsinic acid the succsinic acid pleuropneumoniae ( Actinobacillus succinogenes) F3-ZK, be preserved in Chinese typical culture collection center, preserving number CCTCC NO:M2012036.
- 2. the application of the said CCTCC NO:M2012036 of claim 1 bacterial strain; It is characterized in that: this bacterium is in the 5-15L fermentor tank; Fed-batch fermentation 36-48 h produces succsinic acid 70-95.6 g/L, production intensity 1.70-1.99 g/ (Lh), glucose acid invert ratio 0.70-0.83 g/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210056568 CN102533622B (en) | 2012-03-06 | 2012-03-06 | Actinobacillus succinogenes capable of producing succinic acid |
Applications Claiming Priority (1)
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| CN106801026A (en) * | 2017-02-13 | 2017-06-06 | 广西科学院 | Bacterial strain and its production method used by a kind of utilization xylose mother liquid fermentation succinic acid-producing |
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| CN112111418A (en) * | 2019-06-20 | 2020-12-22 | 华东师范大学 | High-yield strain of succinyl polysaccharide and preparation and application of succinyl polysaccharide |
| CN112574925A (en) * | 2020-12-30 | 2021-03-30 | 广西科学院 | Furfural-tolerant actinobacillus succinogenes GXAS-137FM as well as breeding method and application thereof |
| CN114621897A (en) * | 2022-03-29 | 2022-06-14 | 山东理工大学 | Strain for producing succinic acid, method for producing succinic acid by using strain and application of strain |
| CN114807250A (en) * | 2022-06-08 | 2022-07-29 | 山东飞扬化工有限公司 | Method for producing succinic acid by fermentation |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106801026A (en) * | 2017-02-13 | 2017-06-06 | 广西科学院 | Bacterial strain and its production method used by a kind of utilization xylose mother liquid fermentation succinic acid-producing |
| CN106801026B (en) * | 2017-02-13 | 2020-05-12 | 广西科学院 | Bacterial strain for producing succinic acid by fermentation of xylose mother liquor and production method thereof |
| CN106995794A (en) * | 2017-04-19 | 2017-08-01 | 广西科学院 | A kind of Actinobacillus succinogenes engineered strain and its construction method and purposes for improving succinic acid yield |
| CN106995794B (en) * | 2017-04-19 | 2020-09-18 | 广西科学院 | Succinic acid-producing actinobacillus engineering strain for improving succinic acid yield and construction method and application thereof |
| CN112111418A (en) * | 2019-06-20 | 2020-12-22 | 华东师范大学 | High-yield strain of succinyl polysaccharide and preparation and application of succinyl polysaccharide |
| CN112111418B (en) * | 2019-06-20 | 2022-09-20 | 华东师范大学 | High-yield strain of succinyl polysaccharide and preparation and application of succinyl polysaccharide |
| CN112574925A (en) * | 2020-12-30 | 2021-03-30 | 广西科学院 | Furfural-tolerant actinobacillus succinogenes GXAS-137FM as well as breeding method and application thereof |
| CN114621897A (en) * | 2022-03-29 | 2022-06-14 | 山东理工大学 | Strain for producing succinic acid, method for producing succinic acid by using strain and application of strain |
| CN114621897B (en) * | 2022-03-29 | 2024-02-27 | 山东理工大学 | Strain for producing succinic acid and method for producing succinic acid by strain and application of strain |
| CN114807250A (en) * | 2022-06-08 | 2022-07-29 | 山东飞扬化工有限公司 | Method for producing succinic acid by fermentation |
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