CN102526130A - Application of walking fern and liver function revised tea - Google Patents
Application of walking fern and liver function revised tea Download PDFInfo
- Publication number
- CN102526130A CN102526130A CN2011103482267A CN201110348226A CN102526130A CN 102526130 A CN102526130 A CN 102526130A CN 2011103482267 A CN2011103482267 A CN 2011103482267A CN 201110348226 A CN201110348226 A CN 201110348226A CN 102526130 A CN102526130 A CN 102526130A
- Authority
- CN
- China
- Prior art keywords
- liver
- tea
- liver function
- injury
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to the technical field of traditional Chinese medicine and discloses application of walking fern and liver function revised tea. The liver function revised tea made of the walking fern serving as an active substance has remarkable curative effect on chemical liver injury and pathological liver injury caused by various reasons including alcohol, carbon tetrachloride, paracetamol, dexamethasone combined alcohol, concanavalin A and the like. The fact that the walking fern can be applied to preparation of pharmaceuticals for preventing and treating liver injury caused by various reasons and liver malfunction caused by the liver injury is demonstrated.
Description
Technical field
The present invention relates to the Chinese medicine technical field, be specifically related to application and a kind of liver function correction tea of walking fern.
Background technology
Hepatic injury is the clinical common healthy disease of harm humans.Hepatic injury is exactly the invasion that liver receives extraneous factor, thus the liver damage that causes.Hepatic injury is divided into pathologic hepatic injury and chemical liver injury.Chemical liver injury is meant the hepatic injury that the multiple factor that comprises medicine, poisonous substance causes; And the pathologic hepatic injury mainly comprises the hepatic injury that hepatic injury that first, second, third, fourth, penta 5 kinds of viral hepatitis cause and hepatocarcinoma etc. cause; Hepatic injury takes place can to cause hepatic dysfunction usually, also can cause hepatic fibrosis, even the generation of liver cirrhosis, hepatocarcinoma.
Course of liver damage is relevant with immunity with oxidative stress mostly; Its ability inducing hepatocyte biofilm system generation lipid peroxidation; Energy metabolism in the interference cell; The dead program of activating cell etc.; Finally raise with alanine aminotransferase (ALT) and aspartate aminotransferase and hepatic tissue in transforminggrowthfactor-(TGF-β 1), tissue inhibitor of metalloproteinase (TIMP-1), laminin (LN), and the content that waits of 1-3 Collagen Type VI (Col-I, Col-II, Col-III) to increase be performance, formation hepatic dysfunction in various degree.
Chinese medicine has tangible liver protection function; No matter be mostly chemical composition of Chinese materia medica or Chinese medicine compound are through regulating immunity, improve oxidation resistance, reduce in the histiocyte free radical etc. and safeguard that liver plasma membrane is complete, slow down hepatocellular apoptosis, thereby the liver protecting is avoided damage.Walking fern; Have another name called Herba Camptosori Sibirici, Radix Rhodiolae Yunnanensis, gap bridge grass; Ground herb for Aspleniaceae plant walking fern (Camptosorus sibiricus Rupr); Main product in northeast, ground such as North China, northwest, the Inner Mongol, the successive dynasties book on Chinese herbal medicine is not seen relevant its medicinal record, among the peoplely is used to treat hemiplegia that traumatic hemorrhage, metrorrhagia, thromboangiitis obliterans, neurodermatitis, cerebral embolism cause and diabetic complication etc.Modern pharmacological research shows that walking fern water containing ethanol extraction liquid has dilating effect to rabbit ear blood vessel, Bufo siccus hind leg blood vessel and rabbit limbs hind leg blood vessel, and its effective site is total flavones and organic acid.But, do not seeing any report aspect prevention and the treatment hepatic injury for walking fern.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of new application and a kind of liver function correction tea of walking fern.
Walking fern does not see relevant its medicinal record in the successive dynasties book on Chinese herbal medicine, it is mainly used in hemiplegia that treatment traumatic hemorrhage, metrorrhagia, thromboangiitis obliterans, neurodermatitis, cerebral embolism cause and diabetic complication etc.And the further investigation of walking fern is found that walking fern has tangible reparation and protective effect to hepatic injury and the hepatic insufficiency that a variety of causes causes through the applicant.
Therefore, the present invention proposes the application of walking fern in the hepatic dysfunction medicine for preparing prevention and treatment hepatic injury and cause by hepatic injury.
Hepatic injury according to the invention is preferably chemical liver injury and pathologic hepatic injury.Wherein, As preferably, the chronic hepatic injury that said chemical liver injury causes for the acute liver damage that caused by carbon tetrachloride, by carbon tetrachloride, the acute liver damage that causes by ethanol, the acute liver damage that causes by acetaminophen or the fatty liver that causes by dexamethasone associating ethanol.The immunologic liver injury of said pathologic hepatic injury for causing by concanavalin A, Con A.
In addition, the present invention also provides a kind of liver function correction tea, is made after dried, crushed or by walking fern herb decocting after dried, crushed, decocting extracting solution simmer down to original volume 40-60% promptly gets by the walking fern herb.Liver function correction tea by the walking fern herb makes is after dried, crushed drunk with hot-water soak; By the walking fern herb through oven dry, behind the decocting, the liver function correction tea concentrated extracting solution capable of direct drinking that concentrated extracting solution makes, as preferably, said concentrated percentage ratio is 50%.Both are oral, injection is remarkable to the drug effect of the hepatic injury that a variety of causes causes, can be widely used in preventing and treat hepatic injury and the hepatic dysfunction that causes by hepatic injury in.
Liver function correction tea animal effective dosage ranges according to the invention is oral 0.1-10g/kg (body weight)/day, subcutaneous injection 0.1-5g/kg (body weight)/day, quiet notes 0.1-5g/kg (body weight)/day by the conversion of walking fern herb dry product, and preferred dose is oral 0.25-5g/kg (body weight)/day, subcutaneous injection 0.25-2.5g/kg (body weight)/day, quiet notes 0.25-2.5/kg (body weight)/day.The human effective dosage ranges is oral 0.8-80g/ day, subcutaneous injection 0.8-40g/ day, quiet notes 0.8-40g/ day, and preferred dose is oral 2-40g/ day, subcutaneous injection 2-20g/ day, quiet notes 2-20g/ day.
In the medicine process of research and development prevention and treatment hepatic injury; The liver injury model that is caused by carbon tetrachloride as well known to those skilled in the art can be represented the pathological characters of typical chemical liver injury; Therefore the present invention carries out the test of pesticide effectiveness with high, medium and low 3 dose groups to chmice acute hepatic injury and the mice chronic hepatic injury that is caused by carbon tetrachloride with said liver function correction tea; The result shows; The acute liver damage mice that liver function correction tea according to the invention causes carbon tetrachloride has reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect, the acute liver damage that can significantly stop carbon tetrachloride to cause;
And the chronic hepatic injury mice is also had reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect; Can also significantly reduce transforminggrowthfactor-(TGF-β 1) in the hepatic tissue, tissue inhibitor of metalloproteinase (TIMP-1), laminin (LN), reach 1-3 Collagen Type VI (Col-I; Col-II; Col-III) content equal size improves mice chronic hepatic injury liver cirrhosis pathology state.
In addition; Acute liver damage that the present invention causes to the acute liver damage that caused by ethanol, by acetaminophen with high, medium and low 3 dose groups equally and the fatty liver that is caused by dexamethasone associating ethanol have carried out the test of pesticide effectiveness, and result of the test shows the hepatic injury that liver function correction tea according to the invention can effectively stop various above-mentioned reasons to cause.
The main pathogenic factor of pathologic hepatic injury is that HAV, hepatitis B virus etc. are secreted and to be similar to the same immunogenic substances of concanavalin A, Con A, thereby with hepatocyte immunoreation takes place, the infringement hepatocyte.Therefore; The present invention carries out the test of pesticide effectiveness with high, medium and low 3 dose groups by the immunologic liver injury that concanavalin A, Con A causes with said liver function correction tea equally; The result shows that liver function correction tea test group according to the invention and model group have significant difference; Can effectively prevent immunologic liver injury, show that liver function correction tea according to the invention can prevent and treat the pathologic hepatic injury.
The hepatic injury that can effectively stop multiple reason to cause owing to liver function correction tea according to the invention is so it also has significant curative effect to the hepatic dysfunction that is caused by hepatic injury.
Can know by above technical scheme; The liver function correction tea that with the walking fern is active substance is processed according to the invention all has significant curative effect to chemical liver injury and the pathologic hepatic injury that is caused by multiple reason, show that walking fern can be applied to prepare prevention and treatment hepatic injury and the hepatic dysfunction medicine that causes by hepatic injury in.
Description of drawings
Shown in Figure 1 be in the liver function correction tea dose groups to CCl
4The bar diagram of the acute liver damage mice serum alanine aminotransferase (ALT) that causes, the influence of aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as CCl
4Model group, 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and CCl in the expression liver function correction tea
4Model group has significant difference, P＜0.01;
Shown in Figure 2 be in the liver function correction tea dose groups to CCl
4The bar diagram of the chronic hepatic injury mice serum alanine aminotransferase (ALT) that causes, the influence of aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as CCl
4Model group, 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and CCl in the expression liver function correction tea
4Model group has significant difference, P＜0.01;
Shown in Figure 3 be in the liver function correction tea dose groups to CCl
4The bar diagram of the influence of transforminggrowthfactor-in the chronic hepatic injury murine liver tissue that causes (TGF-β 1), tissue inhibitor of metalloproteinase (TIMP-1) and laminin (LN) content;
Wherein, 1 is depicted as the normal control group, and 2 are depicted as CCl
4Model group, 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and CCl in the expression liver function correction tea
4Model group has significant difference, P＜0.01;
*Dose groups and CCl in the expression liver function correction tea
4Model group has significant difference, P＜0.05;
Shown in Figure 4 be in the liver function correction tea dose groups to CCl
41-3 Collagen Type VI in the acute liver damage murine liver tissue that causes (Col-I, Col-II, Col-III) bar diagram of the influence of content;
Wherein, 1 is depicted as the normal control group, and 2 are depicted as CCl
4Model group, 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and CCl in the expression liver function correction tea
4Model group has significant difference, P＜0.05;
The bar diagram of the influence of the acute liver damage mice serum alanine aminotransferase (ALT) that acetaminophen is caused for dose groups in the liver function correction tea shown in Figure 5, aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as the acetaminophen model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and acetaminophen model group have significant difference, P＜0.01 in the expression liver function correction tea;
The bar diagram of the influence of the acute liver damage mice serum alanine aminotransferase (ALT) that ethanol is caused for dose groups in the liver function correction tea shown in Figure 6, aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as the ethanol model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and ethanol model group have significant difference, P＜0.01 in the expression liver function correction tea;
Shown in Figure 7 is the bar diagram of the influence of dose groups causes ethanol in the liver function correction tea acute liver damage murine liver tissue catalase (CAT), superoxide dismutase (SOD) and malonaldehyde (MDA) content;
Wherein, 1 is depicted as the normal control group, and 2 are depicted as the ethanol model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and ethanol model group have significant difference, P＜0.05 in the expression liver function correction tea;
The bar diagram of the influence of the immunologic liver injury mice serum alanine aminotransferase (ALT) that concanavalin A, Con A (ConA) is caused for dose groups in the liver function correction tea shown in Figure 8, aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as the ConA model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and ConA model group have significant difference, P＜0.01 in the expression liver function correction tea;
The bar diagram of the influence of the fatty liver mice serum alanine aminotransferase (ALT) that dexamethasone associating ethanol is caused for dose groups in the liver function correction tea shown in Figure 9, aspartate aminotransferase (AST);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as dexamethasone-ethanol model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and dexamethasone-ethanol model group have significant difference, P＜0.01 in the expression liver function correction tea;
The bar diagram of the influence of fatty liver mice serum T-CHOL (TC) that dexamethasone associating ethanol is caused for dose groups in the liver function correction tea shown in Figure 10 and triglyceride (TG);
Wherein, 1 is depicted as the normal control group, and 2 are depicted as dexamethasone-ethanol model group, and 3 are depicted as dose groups in the liver function correction tea;
*Dose groups and dexamethasone-ethanol model group have significant difference, P＜0.01 in the expression liver function correction tea;
The specific embodiment:
The invention discloses application and a kind of liver function correction tea of walking fern, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention and application are described through preferred embodiment; The related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
Application and a kind of liver function correction tea with regard to walking fern provided by the invention further specifies below.
Embodiment 1: liver function correction tea according to the invention is to the influence of carbon tetrachloride chmice acute hepatic injury
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week, 10 every group, male and female half and half are respectively: normal control group, carbon tetrachloride model group, liver function correction tea low dosage, middle dosage, high dose group.
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.Every 30g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the high dose group medicinal liquid; Every 15g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is middle dose groups medicinal liquid; Every 7.5g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the low dose group medicinal liquid; Three groups of volume, temperature and soak times that add water are all consistent.
Normal control group and carbon tetrachloride model group are irritated the normal saline of stomach 10ml/kg, and other each dose groups is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 10 days.1h after the 9th administration, each organizes the CCl of mouse peritoneal injection 10%
4Olive oil solution 0.2ml/ is (normal group is only injected with the capacity olive oil) only.CCl
4After the injection, water 24h (1h after the last administration) is can't help in fasting.Mice is extractd eyeball get blood, preparation serum builds up the alanine aminotransferase (ALT) that bio-engineering research produces with Nanjing and detects Serum ALT, AST activity with aspartate amino transferase (AST) test kit, and the result sees Fig. 1.Put to death mice, get the part hepatic tissue immediately, place 10% formalin to fix, preparation histopathologic slide, hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
Lumbar injection CCl
4Can cause the chmice acute hepatic injury, the Cytochrome P450 in the liver can be with CCl
4Activate and be CCl
3And Cl, these free radicals can destroy the liver cell membrane structure through complex series of reactions, and its permeability is increased, and cause transaminases such as ALT, AST to infiltrate blood, make transaminase's in the serum active significantly rising.After irritating the liver function correction tea of stomach various dose, active obviously reduce (see Fig. 1, only do representative with 1.5g/kg dosage treatment group among the figure) of acute liver damage mice ALT and AST do not have significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
The pathology detection testing result shows that normal control group mouse liver color and luster is dark red, and tunicle is smooth, and the liver edge contour is clear, the no any speckle in surface; Mirror is observed visible hepatic tissue structural integrity down, and hepatocyte does not see degeneration, necrosis.
CCl
4The model group liver volume increases, and color is turned white, and is pink colour, and there is tangible mottled decorative pattern on the surface and bleeds profusely a little, and there is congestion the part, tarnish, have with the slight adhesion of adjacent organs; It is unclear that mirror is observed visible lobules of liver boundary down, and the hydropic degeneration of hepatocyte popularity has large stretch of focal necrosis district in the lobules of liver.
Each dose groups mouse liver of liver function correction tea all is rediance, and there is a small amount of petechia on the surface, and is glossy, the lobe of the liver clear-cut, and amount of speckle obviously is less than CCl
4Model group.Mirror is observed visible lobules of liver clear in structure, complete down, the slight hepatic cell cloudy swelling, and cell infiltration obviously alleviates, and kitchen range shape necrotic extent obviously reduces.
Embodiment 2: liver function correction tea according to the invention is to the influence of carbon tetrachloride mice chronic hepatic injury
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week, 10 every group, male and female half and half are respectively: normal control group, carbon tetrachloride model group, liver function correction tea low dosage, middle dosage, high dose group (0.75g/kg, 1.5g/kg, 3g/kg).
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.The every 30g coarse powder of high dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the high dose group medicinal liquid; The middle every 15g coarse powder of dose groups adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is middle dose groups medicinal liquid; The every 7.5g coarse powder of low dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the low dose group medicinal liquid; Three groups of volume, decocting time and cycles of concentration that add water are all consistent.
Normal control group and carbon tetrachloride model group are irritated the normal saline of stomach 10ml/kg, and other each group is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 4 weeks.Except that the normal control group, all the other respectively organize mice all from the on-test, give 40%CCl
4An olive oil solution 0.05ml/ subcutaneous injection, 2 times weekly (Monday and Thursday each 1 time), totally 4 weeks.The olive oil of normal control group subcutaneous injection equivalent, number of times is identical.Water 24h (omnidistance administration finishes back 24h) is can't help in the mice fasting after the last administration; Pluck eyeball and get blood; Centrifuging and taking serum; Build up with Nanjing that alanine aminotransferase (ALT) and aspartate amino transferase (AST) test kit that bio-engineering research produces detects Serum ALT, AST is active, the result sees Fig. 2.
Put to death mice; Get the part liver and prepare liver tissue homogenate; With the transforminggrowthfactor-(TGF-β 1) of import packing, tissue inhibitor of metalloproteinase (TIMP-1), laminin (LN), and 1,3,4 Collagen Type VI (Col-I; Col-III, Col-IV) the ELISA test kit detects transforminggrowthfactor-(TGF-β 1) in the hepatic tissue, tissue inhibitor of metalloproteinase (TIMP-1), laminin (LN), reaches 1-3 Collagen Type VI (Col-I, Col-II; Col-III) content, the result sees Fig. 3, Fig. 4.
Other gets the part hepatic tissue, places 10% formalin to fix, preparation histopathologic slide, and hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
Multiple injection CCl
4Can cause mouse liver chronic injury fibrosis lesion, follow transaminase's in the serum activity to raise simultaneously.The liver function correction tea of irritating the stomach various dose is to CCl
4The chronic hepatic injury mice that causes all has reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect (to see Fig. 2; Only do representative among the figure) with 1.5g/kg dosage treatment group; Can significantly reduce transforminggrowthfactor-in the hepatic tissue (TGF-β 1), tissue inhibitor of metalloproteinase (TIMP-1), laminin (LN), reach 1-3 Collagen Type VI (Col-I; Col-II; Col-III) content (see Fig. 3, Fig. 4, only do representative with 1.5g/kg dosage treatment group among the figure) does not have significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
Pathology detection result shows, normal control group mouse liver smooth surface, and color is dark red, and is glossy, and matter is soft; Mirror is observed visible lobules of liver down, the portal area structure is normal, the lobules of liver structural integrity, and hepatocyte is arranged and is strand, fat-free change, necrosis and cell infiltration.
CCl
4The model group liver surface is uneven, sees tiny grains of sand shape granule, and matter is crisp, and glossiness is not good enough; Mirror is observed a large amount of lipid cavitys of visible hepatic tissue down and is formed; Diffusivity bulla property fat becomes; The visible massive inflammatory cells infiltrated in a large amount of cell infiltration and downright bad portal area and fibrous septum, kiernan's space is at a distance from obviously broadening and a large amount of collagen fiber depositions, type of having pseudolobuli spline structure in the liver.
It is still smooth that liver function correction tea is respectively organized the mouse liver surface, and grains of sand shape granule obviously reduces.Mirror is observed lesion degree down and is obviously reduced than model group lipid cavity, and lobules of liver is complete basically, and the proliferation of fibrous tissue of remnant normal liver cell is not obvious.
Embodiment 3: liver function correction tea according to the invention causes the influence of chmice acute hepatic injury to acetaminophen
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week, 10 every group, male and female half and half are respectively: normal control group, acetaminophen model group, liver function correction tea low dosage, middle dosage, high dose group (0.75g/kg, 1.5g/kg, 3g/kg).
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.Every 30g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the high dose group medicinal liquid; Every 15g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is middle dose groups medicinal liquid; Every 7.5g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the low dose group medicinal liquid; Three groups of volume, temperature and soak times that add water are all consistent.
Normal control group and acetaminophen model group are irritated the normal saline of stomach 10ml/kg, and other each group is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 10 days.1h after the 9th administration, each organizes acetaminophen solution 0.2ml/ (except the normal control group) of mouse peritoneal injection 3%.After the acetaminophen injection, water 24h (1h after the last administration) is can't help in fasting.Mice is extractd eyeball get blood, preparation serum builds up the alanine aminotransferase (ALT) that bio-engineering research produces with Nanjing and detects Serum ALT, AST activity with aspartate amino transferase (AST) test kit, and the result sees Fig. 5.
Put to death mice, get the part hepatic tissue immediately, place 10% formalin to fix, preparation histopathologic slide, hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
After acetaminophen got in the human body, excessive toxic metabolite product combined with cell membrane, causes cell membrane lipid peroxidization, destroyed the intracellular calcium balance, caused the death of cell, and the serum transaminase level significantly raises.The acute liver damage mice that the liver function correction tea of filling stomach various dose causes acetaminophen all has reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect (to see Fig. 5; Only do representative among the figure with 1.5g/kg dosage treatment group), there is not significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
Pathology detection result shows that normal control group Mouse Liver leaflet structure is clear, examines big and clear; Kytoplasm is abundant; Contain the agglomerate bsaophilic cmponent, it is neat that hepatic cords is radial arrangement around central vein, and sinus hepaticus is normal; Karyon clear in structure, hepatocyte do not have the pathological phenomenon of degeneration, necrosis, hyperemia and cell infiltration.
Acetaminophen model group mice; Liver has tangible hyperemia and cavity appearance to become, and the acidophilia becomes and increases, and sinus hepaticus disappears; Hepatocyte around the liver lobule central vein has degeneration and necrosis in various degree; Karyon pyknosis or dissolved fragmentation, inflammatory cell infiltrations such as companion's neutrophilic granulocyte, mononuclear cell, lymphocyte, the hepatic cords normal morphology is destroyed.
It is not obvious that liver function correction tea is respectively organized mouse liver hyperemia, and the hepatocyte cloudy swelling obviously alleviates, and sinus hepaticus broadens, and inflammatory cell infiltration obviously reduces, and liver cell regeneration is obvious.
Embodiment 4: liver function correction tea according to the invention causes the influence of chmice acute hepatic injury to ethanol
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week, 10 every group, male and female half and half are respectively: normal control group, ethanol model group, liver function correction tea low dosage, middle dosage, high dose group (0.75g/kg, 1.5g/kg, 3g/kg).
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.The every 30g coarse powder of high dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the high dose group medicinal liquid; The middle every 15g coarse powder of dose groups adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is middle dose groups medicinal liquid; The every 7.5g coarse powder of low dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the low dose group medicinal liquid; Three groups of volume, decocting time and cycles of concentration that add water are all consistent.
Normal control group and ethanol model group are irritated the normal saline of stomach 10ml/kg, and other each group is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 10 days.1h after the 9th administration, each is organized mouse peritoneal and injects commercially available 52 ° Luzhou Old Cellar Chinese liquor 0.2ml/ only (except the normal group).After the Chinese liquor injection, water 24h (1h after the last administration) is can't help in fasting.Mice is extractd eyeball get blood, preparation serum builds up the alanine aminotransferase (ALT) that bio-engineering research produces with Nanjing and detects Serum ALT, AST activity with aspartate amino transferase (AST) test kit, and the result sees Fig. 6.
Put to death mice; Get the part liver and prepare liver tissue homogenate; Build up catalase (CAT), superoxide dismutase (SOD) and malonaldehyde (MDA) test kit that bio-engineering research produces with Nanjing and detect catalase (CAT), superoxide dismutase (SOD) and malonaldehyde (MDA) content in the hepatic tissue, the result sees Fig. 7.
Other gets the part hepatic tissue, places 10% formalin to fix, preparation histopathologic slide, and hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
The mice alcoholic liver injury is the animal model that the excessive consumption of alcohol of simulation human body causes liver injury, is reduced to the form of expression with transaminase's rising and antioxidase.The acute liver damage mice that the liver function correction tea of filling stomach various dose causes ethanol all has reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect (to see Fig. 6; Only do representative among the figure) with 1.5g/kg dosage treatment group; Can increase hepatic tissue catalase (CAT) and superoxide dismutase (SOD); Reduce hepatic tissue malonaldehyde (MDA) content and (see Fig. 7; Only do representative among the figure with 1.5g/kg dosage treatment group), there is not significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
Pathology detection result shows that normal control group Mouse Liver leaflet structure is clear, cell rope marshalling, and hepatocyte does not have obvious pathological changes.
Ethanol model group hepatic tissue structure disturbance, the lobule boundary is unclear, and sinus hepaticus narrows down, and hepatocyte occurs being dispersed in and the change of lamellar balloon appearance, visible swelling of liver cell and inflammatory cell infiltration.
It is clear basically that liver function correction tea is respectively organized mouse liver lobule boundary, and swelling of liver cell and inflammatory cell infiltration phenomenon obviously alleviate, and sinus hepaticus broadens, and the hepatic tissue structural arrangement is near normal.
Embodiment 5: liver function correction tea according to the invention causes the influence of mouse immune liver damage to concanavalin A, Con A (ConA)
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week, 10 every group, male and female half and half are respectively: normal control group, ConA model group, liver function correction tea low dosage, middle dosage, high dose group (0.75g/kg, 1.5g/kg, 3g/kg).
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.Every 30g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the high dose group medicinal liquid; Every 15g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is middle dose groups medicinal liquid; Every 7.5g coarse powder adds the new system boiled water 100ml that boils and soaks the 15min after-filtration, and gained solution is the low dose group medicinal liquid; Three groups of volume, temperature and soak times that add water are all consistent.
Normal control group and ConA model group are irritated the normal saline of stomach 10ml/kg, and other each group is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 10 days.1h after the last administration, each organizes mouse tail vein injection ConA 15mg/kg (except the normal group).Mice is plucked the eyeball blood sampling behind the injection ConA 8h, and separation of serum builds up the alanine aminotransferase (ALT) that bio-engineering research produces with Nanjing and detects Serum ALT, AST activity with aspartate amino transferase (AST) test kit, and the result sees Fig. 8.
Put to death mice, get the part hepatic tissue immediately, place 10% formalin to fix, preparation histopathologic slide, hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
ConA injection back causes immunologic liver injury through activated T lymphocytes, is to be fit to the pathomechanism of research human virus hepatitis, autoimmune liver disease and the animal model that carries out the anti-liver injury medicament screening.The immunologic liver injury mice that the liver function correction tea of filling stomach various dose causes concanavalin A, Con A (ConA) all has reduction serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) effect (to see Fig. 8; Only do representative among the figure with 1.5g/kg dosage treatment group), there is not significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
Pathology detection result shows, visible a plurality of lobules of liver in the normal mouse, liver, and the liver rope is arranged regular, the clear in structure of lobules of liver, the portal area structure is clear, does not have obvious cell infiltration and hepatic necrosis.
ConA model group mice, liver rope arrangement disorder, the lobules of liver structural deterioration has than multi-lymphocytes and monocyte infiltration, visible special mess shape hepatic necrosis.
Liver function correction tea is respectively organized mice, and the lobules of liver structure keeps basically, and the liver rope is arranged regular basically, and there are a small amount of lymphocyte, monocyte infiltration in the portal area, does not see obvious hepatic necrosis, and pathological changes obviously alleviates than model control group.
Embodiment 6: liver function correction tea according to the invention causes the influence of mice fatty liver to dexamethasone associating ethanol
1, test method
Be divided into 5 groups at random after 50 kunming mice adaptabilities were fed for 1 week; Every group 10; Male and female half and half are respectively: normal control group, dexamethasone-ethanol model group, liver function correction tea low dosage, middle dosage, high dose group (0.75g/kg, 1.5g/kg, 3g/kg).
Walking fern herb aerial parts is cleaned, oven dry, pulverized the back and cross 40 mesh sieves to get coarse powder subsequent use.The every 30g coarse powder of high dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the high dose group medicinal liquid; The middle every 15g coarse powder of dose groups adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is middle dose groups medicinal liquid; The every 7.5g coarse powder of low dose group adds water 200ml and boils the 15min after-filtration, and simmer down to 100ml extracting solution is the low dose group medicinal liquid; Three groups of volume, decocting time and cycles of concentration that add water are all consistent.
Normal control group and dexamethasone-ethanol model group is irritated the normal saline of stomach 10ml/kg, and other each group is irritated the relative medicine of the equal capacity of stomach, every day 1 time, continuous 9 days.From the 1st day, 1h only irritated the commercially available 35 ° Luzhou Old Cellar Chinese liquor 0.2ml/ of stomach after every group of mice administration except that the normal control group, every day 1 time, continuous 9 days.Played every group of Luzhou Old Cellar Chinese liquor pneumoretroperitoneum injection dexamethasone injection 10mg/kg that mouse stomach is commercially available 35 ° except that the normal control group, every day 1 time, continuous 4 days on the 5th day.After omnidistance modeling administration finishes; Fasting can't help water 24h and mice is extractd eyeball is got blood; Preparation serum; Build up the alanine aminotransferase (ALT) that bio-engineering research is produced with Nanjing, aspartate amino transferase (AST), T-CHOL (TC) and triglyceride (TG) test kit detect Serum ALT, AST activity and TC, TG content, and the result sees Fig. 9, Figure 10.
Put to death mice, get the part hepatic tissue immediately, place 10% formalin to fix, preparation histopathologic slide, hematoxylin-eosin staining, microscopically are observed pathology and are changed.
2, result
Dexamethasone associating ethanol mice fatty liver model shows as blood fat and raises, and occurs the abnormal liver function that the transaminase raises simultaneously.The fatty liver mice that the liver function correction tea of filling stomach various dose causes dexamethasone associating ethanol all has reduction serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), T-CHOL (TC) and triglyceride (TG) effect (to see Fig. 9, Figure 10; Only do representative among the figure with 1.5g/kg dosage treatment group), there is not significant difference between all the other each dosage and 1.5g/kg dosage treatment group.
Pathology detection result shows, the normal group mice, and lobules of liver is high-visible, and sinus hepaticus is high-visible, liver rope marshalling, the fat-free degeneration of hepatocyte, cellular morphology Non Apparent Abnormality.
Dexamethasone-ethanol model mice, the hepatic cell fattydegeneration of bulla property appears in liver, and the hepatocyte of degeneration is filled the air and involves nearly all lobules of liver, and swelling of liver cell is rounded, and nucleus is pressed on one side, is full of the significant quantities of fat cavity in the endochylema, and boundary is unclear.
Liver function correction tea is respectively organized mice, and the liver fat degeneration obviously improves, and fat drips and reduces or disappearance, and it is normal that the hepatocyte structure is recovered basically.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (6)
1. the application of walking fern in the hepatic dysfunction medicine for preparing prevention and treatment hepatic injury and cause by hepatic injury.
2. according to the said application of claim 1, it is characterized in that said hepatic injury is chemical liver injury and pathologic hepatic injury.
3. according to the said application of claim 2; It is characterized in that the chronic hepatic injury that said chemical liver injury causes for the acute liver damage that caused by carbon tetrachloride, by carbon tetrachloride, the acute liver damage that causes by ethanol, the acute liver damage that causes by acetaminophen or the fatty liver that causes by dexamethasone associating ethanol.
4. according to the said application of claim 2, it is characterized in that the immunologic liver injury of said pathologic hepatic injury for causing by concanavalin A, Con A.
5. a liver function correction tea is characterized in that, is made after dried, crushed or by walking fern herb decocting after dried, crushed, decocting extracting solution simmer down to original volume 40-60% promptly gets by the walking fern herb.
6. according to the said liver function correction of claim 5 tea, it is characterized in that said concentrated percentage ratio is 50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110348226 CN102526130B (en) | 2011-11-07 | 2011-11-07 | Application of walking fern and liver function revised tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110348226 CN102526130B (en) | 2011-11-07 | 2011-11-07 | Application of walking fern and liver function revised tea |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102526130A true CN102526130A (en) | 2012-07-04 |
| CN102526130B CN102526130B (en) | 2013-10-23 |
Family
ID=46334951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110348226 Expired - Fee Related CN102526130B (en) | 2011-11-07 | 2011-11-07 | Application of walking fern and liver function revised tea |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102526130B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102935096A (en) * | 2012-11-26 | 2013-02-20 | 河南大学 | Application of camptosorus sibiricus total flavonids in preparation of medicines for preventing and treating tumour |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538296A (en) * | 2008-03-17 | 2009-09-23 | 沈阳药科大学 | Active ingredients of camptosorus sibiricus, and extraction method and use of same |
-
2011
- 2011-11-07 CN CN 201110348226 patent/CN102526130B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538296A (en) * | 2008-03-17 | 2009-09-23 | 沈阳药科大学 | Active ingredients of camptosorus sibiricus, and extraction method and use of same |
Non-Patent Citations (4)
| Title |
|---|
| LI NING: "A new cycloartane glycoside from camptosorus sibiricus", 《FITOTERAPIA》, vol. 79, no. 4, 30 June 2008 (2008-06-30), pages 314 - 316 * |
| 南京中医药大学: "《中药大辞典（上册）》", 31 March 2006, article "马蹬草", pages: 418 * |
| 李宁: "过山蕨中黄酮类成分的研究", 《中国药学杂志》, vol. 42, no. 14, 22 July 2007 (2007-07-22), pages 1049 - 1052 * |
| 毛颖婕: "肝损伤的中药治疗研究进展", 《江西中医学院学报》, vol. 23, no. 1, 15 February 2011 (2011-02-15), pages 91 - 94 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102935096A (en) * | 2012-11-26 | 2013-02-20 | 河南大学 | Application of camptosorus sibiricus total flavonids in preparation of medicines for preventing and treating tumour |
| CN102935096B (en) * | 2012-11-26 | 2014-07-16 | 河南大学 | Application of camptosorus sibiricus total flavonids in preparation of medicines for preventing and treating tumour |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102526130B (en) | 2013-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101721488B (en) | Pharmaceutical composition for treating liver diseases and prepration method thereof | |
| CN101357219A (en) | Medicine for treating chronic hepatitis b | |
| CN105770514A (en) | Anti-radiation black tea composition and preparation method thereof | |
| CN109528848A (en) | A kind of pharmaceutical composition and the preparation method and application thereof preventing and treating alcoholic liver injury | |
| CN102784248B (en) | Chinese medicine composition for preventing and treating alcoholic liver injury | |
| CN101664482A (en) | Externally-applied Zhuang medicine for treating beriberi and skin pruritus and preparation method thereof | |
| CN105125601A (en) | Penthorum chinense pursh extract and preparing method and application thereof | |
| CN102743424A (en) | Flos chrysanthemi indici effective ingredient and application thereof | |
| CN102526130B (en) | Application of walking fern and liver function revised tea | |
| CN103719496B (en) | A kind of leaves of Hawthorn lowering blood-fat and reducing weight health protection tea and preparation method thereof | |
| CN101468155A (en) | Chinese medicinal composition for treating dementia, as well as preparation method, preparation and use thereof | |
| CN109925445A (en) | Anoectochilus causes the application in acute liver drug in preparation prevention and/or treatment D-Gal | |
| CN102462710A (en) | Application of sunset abelmoschus flower total flavone to preparation of medicament for preventing and treating hepatofibrosis | |
| CN103623109B (en) | Traditional Chinese medicine composition for treating ischemic cerebrovascular disease and preparation method thereof | |
| CN114010736A (en) | Traditional Chinese medicine composition for regulating anxiety, depression and insomnia and preparation method thereof | |
| CN102293847A (en) | Chinese medicinal composition for treating constipation, acne and hyperlipidemia, and preparation method | |
| CN102188500B (en) | Traditional Chinese medicine preparation for treating anxiety neurosis | |
| CN106578185A (en) | Jiangjunhong blood fat lowering tea and preparation method thereof | |
| CN102641349B (en) | Traditional Chinese medicine composite for resisting ephedrine damage and production technology thereof | |
| CN114767760B (en) | A composition with liver protecting effect | |
| Wang et al. | Annual advances of Chinese minority traditional medicine in 2020 | |
| Chandrashekar et al. | Hepatoprotective activity of Leucas lavandulaefolia against carbon tetrachloride-induced hepatic damage in rats | |
| CN114848764B (en) | Traditional Chinese medicine compound composition for preventing and treating liver injury and preparation method and application thereof | |
| CN110123859B (en) | Chrysanthemum leaf extract for preventing and treating liver injury and application thereof | |
| CN106511829A (en) | Yao medicine tub-bath preparation capable of reducing blood pressure, blood sugar and blood fat, and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131023 Termination date: 20171107 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |