CN102524525A - Composite microbial fermented soybean hull feed and preparation method thereof - Google Patents
Composite microbial fermented soybean hull feed and preparation method thereof Download PDFInfo
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- CN102524525A CN102524525A CN2012100185687A CN201210018568A CN102524525A CN 102524525 A CN102524525 A CN 102524525A CN 2012100185687 A CN2012100185687 A CN 2012100185687A CN 201210018568 A CN201210018568 A CN 201210018568A CN 102524525 A CN102524525 A CN 102524525A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention belongs to the field of breeding feed, solves the problem that milk is polluted by aflatoxin because corn serves a raw material in the conventional milk cow feed and provides composite microbial fermented soybean hull feed and a preparation method thereof. The composite microbial fermented soybean hull feed is prepared by using soybean hull as a raw material, adding byproduct bean pulp of soybean processing and combining with saccharomycetes, lactic acid bacteria, bacillus, bifidobacterium composite bacteria. The feed has good palatability and milk cows like to eat the feed. The feed can maintain the gastrointestinal flora stable, improve disease resistance of milk cows and improve quality of milk.
Description
Technical field
The invention belongs to the aquaculture field of fodder, be specifically related to a kind of biological feedstuff of processing with the compound microorganism ferments soybean skin and preparation method thereof.
Background technology
At present, along with the raising of living standards of the people, the demand of milk is increasing, and the breed amount of milk cow day by day all increases, the milk cow forage also growing tension of originating.Corn is the main feed of milk cow; The pollution of aspergillus flavus often takes place in corn in storage process; Milk cow is fed and has the corn of aflatoxin, and aflatoxin is gone out through the milk metabolism, and the residual direct threats of aflatoxin the people's health in the milk.Therefore, develop new feed resource, the residual of aflatoxin is a problem demanding prompt solution in the milk to avoid.
Soybean skin is the byproduct of soybean oil-producing technique, accounts for 10% of whole soybean volume, accounts for 8% of whole soybean weight.Soybean skin mainly is the material that the soybean skin encapsulates; Color is an ecru or light yellow; Process hot method decortication or two kinds of processing method gained of crushing screen by grease; Main component is cell membrane and string, and crude fiber content is 38%, crude protein content is 12.2%, calcium oxide content is 0.53%, phosphorus content is 0.18%, content of lignin is lower than 2% (NRC, 1996).Adopt soybean skin as the existing many reports of the method for feedstock production milk cow forage; Like CN200810036991.3, CN201010103093.2; Yet; In these reports, also need adopt steam-flaked corn, maize alcohol stillage or defatted rice bran, these raw materials still may be brought aflatoxin contamination into to feed.
In addition; Prepare in the method for feed existing, be the digesting and assimilating and strengthen that livestock is disease-resistant, the diseases prevention ability of the nutritional labeling that improves feed, promotion livestock, how in feed, to add bacterial classification; Add single bacterial classification and can not play tangible effect; Increase feed preparation technology's complexity on the contrary, improved the cost of feed, caused the waste of resource.
The application does not use any corn and byproduct thereof as raw material, adopts soybean skin as raw material, adds the byproduct dregs of beans of soybean processing; Combining yeast bacterium, lactic acid bacteria, bacillus and Bifidobacterium composite bacteria have prepared a kind of compound microorganism ferments soybean skin feed; This feed good palatability, milk cow are liked searching for food, and it is stable to keep gastrointestinal bacterial flora, improve the resistance against diseases of milk cow; Stop or alleviate the disease generation of milk cow, improve the quality of milk.
Summary of the invention
For solving prior art problems, a kind of compound microorganism ferments soybean skin feed is provided, its proportioning raw materials that adopts is: 800 kilograms of soybean skin; 200 kilograms of dregs of beans; Molasses 10-20 kilogram, and saccharomycete (content is: saccharomycete >=200,000,000/gram) 2-2.5 kilogram, and (content is lactic acid bacteria: lactic acid bacteria >=300,000,000/gram) 1-3 kilogram; (content is: bacillus >=300,000,000/gram) 1.5-3.5 kilogram, (content is Bifidobacterium bacillus: Bifidobacterium >=200,000,000/gram) 2-3 kilogram.
The concrete steps that prepare this soybean skin feed are: (1) is placed on molasses 10-20 kilogram in about about 500 kilograms stainless steel cask; Adding 45 ℃ of warm water evenly dissolves and stirs and be blended into saccharomycete 2-2.5 kilogram, lactic acid bacteria 1-3 kilogram, bacillus 1.5-3.5 kilogram, Bifidobacterium 2-3 kilogram after 30 minutes; Evenly stir after 30-50 minute sealing place 48 hours for use; The temperature of stainless steel cask should keep processing mixed bacteria liquid between 25-45 ℃ during this time; (2) 200 kilograms of dregs of beans, 800 kilograms of skin of beancurd homogeneous powders are broken to the 40-80 order and process dregs of rice skin mixture; (3) mixed bacteria liquid evenly is sprayed in the dregs of rice skin mixture, moisture Control is about 45-55%, and the biological feedstuff packaging bag of packing into, is put into the greenhouse and fermented by 25 or 40 kilograms every bag; (4) fermentation temperature was controlled at 20-30 ℃ in the 1st day to the 4th day, was controlled at about 45 ℃ in the 5th day to the 8th day, was controlled at about 25 ℃ in the 9th day to the 10th day; Pack into after will fermenting in (5) the 10th days the finished product detection metering successful and put in storage in the polybag of sealing.
Above-mentioned soybean skin can adopt the soybean skin product to replace, and this soybean skin product is that soy meal ground feed and soybean are pulverized waste material.The soy meal ground feed is made up of the processed soybean powder and the particle of soybean skin and pulverizer afterbody.Soybean skin is pulverized waste material and partly is made up of soybean skin and the cotyledon that adheres in the dried shell.The crude protein content 13% of soy meal ground feed, crude fiber content are about 32%.Soybean skin is pulverized the crude protein content 11% of waste material, and crude fiber content is about 35%.
The nutritional labeling of soybean skin is: crude fibre 38%, and crude protein 12%, crude fat 2.45% calcium oxide 0.53%, phosphorus 0.18% woodenly is lower than 2%.The crude protein content of soybean skin is than corn high by 2.2% (crude protein content of corn is 9.8%); Net energy is 8.15MJ/kg, is lower than the 8.23MJ/kg of corn, is higher than the 6.72MJ/kg of wheat bran.Therefore; Adopt soybean skin instead of part grain trough, substitute ruminant (like cattle and sheep) part concentrate supplement, can reduce the cud acid poisoning that causes because of high-precision feed diet; And soybean skin can stimulate in the gastric juice and can grow fast by cellulolytic microorganism, the vigor of enhancing degradation of fibers.
Soybean skin contains a large amount of crude fibres, can replace low-quality stalk and hay in the plant-eating animal roughage.The stalk palatability is poor, and crude protein content, content of mineral substances are few, and wooden rope content is high.The herbage solarization is being processed in the process of hay, because chemical action and mechanism, nutrient loss is more than half, and the plant-eating animal utilization rate is low.Soybean skin NDF (neutral detergent fiber) accounts for 63%, and ADF (acid detergent fiber) accounts for 47%, and content of lignin is merely 1.9%.Cellulosic water quality degree is the key factor of fiber rope digestion height in the feed, and degree of lignification is very low owing to the crude fiber content height of soybean skin, so soybean skin can replace stalk and hay.HSU (1987) result of the test shows: soybean skin dry 27h, and Nylon Bag digestibility (measuring a kind of detection method of ruminant digestibility) is 90.3%, 36-48h can be by catapepsis.Owen (1987) result of the test shows: but the NDF digestibility of soybean skin is up to 95%.Kedey and Williams (1995) point out that digestible fibroid byproduct is the good roughage of winter range, is superior to feeding in the winter time hay.
The soybean lecithin that contains in the soybean skin is a kind of mixed phosphatide, is by phosphatid ylcholine (lecithin is called for short PC), phosphatidyl-ethanolamine (cephalin; Abbreviation PE), phosphatidylinositols (lipositol is called for short PI) is formed functions of treating hyperlipidemia, high cholesterol; Improve body immunity; And can protect liver, the cleaning blood vessel is eliminated vivotoxin.
Soyabean oligosaccharides in the soybean skin is that molecular structure is connected with glycosidic bond by 2-10 monose molecule and the carbohydrate general name that forms; Be to be of value to one type of healthy compound sugar of stomach; Can impel the breeding of Bifidobacterium, suppress intrinsic putrefactivebacteria growth and breeding in external source pathogenic bacteria and the enteron aisle, the milk cow enteron aisle is had dual regulation; Constipation and diarrhoea are regulated in prevention, improve the body resistance against diseases of milk cow.
Soya-bean polypeptides in the soybean skin can have the effect of preventing and curing diseases, regulate physiological function, can directly get into animal body and need not digest direct absorption, promotes energetic supersession; Increase the endurance of muscle; Improve milk cow immunity and improve the milk cow resistance against diseases, promote growth of microorganism to grow, improve gastrointestinal function.
Above-mentioned lactic acid bacteria is meant that the fermenting carbohydrate primary product is the general name of one type of no gemma gram-positive bacterium of lactic acid.Can suppress the spoilage organisms breeding, control enteron aisle abdominal distension, diarrhoea promote the absorption of nutriments such as protein, monose and calcium magnesium, regulate the useful variation of stomach flora, suppress aflatoxin and generate enhance immunity power and resistance.
The saccharomycete that the application adopts is unicellular fungi, can promote growing of animal, shortens feeding period, increases meat amount and milk yield, improves meat and milk quality, strengthens the resistance against diseases of cub.
Bifidobacterium is the Gram-positive bacillus of anaerobism, can safeguard the colony balance of enteron aisle normal bacteria, suppresses growth of pathogenic bacteria; Prevent constipation diarrhea gastrointestinal disease, antitumor, and can be in enteron aisle synthetic vitamin, amino acid; Improve body to nutrient absorbing; Improve the anti-lactose property of dairy products, improve digestibility, the enhancing body immunity function.
Bacillus is used to produce soya-bean polypeptides, gramicidins isoreactivity material, and pathogenic bacteria are had the obvious suppression effect.
The composition of the compound microorganism ferments soybean skin feed that the present invention makes is: fiber >=34%, protein >=13%, compound beneficial bacterium sum >=200,000,000/g, compound sugar, isoflavones, lecithin etc.; Preferably deposit in aeration-drying, shady and cool place; In order to avoid pollute, go bad, the shelf-life can reach 6 months.
This compound microorganism ferments soybean skin feed adds 8-15% in use in batch, and controls the every cow head 1-1.5 kilogram of feeding every day, reduces simultaneously with the amount fine fodder.
The milk cow of this soybean skin feed of feeding is liked searching for food, feed intake is big, and digestibility is high, improves efficiency of feed utilization about 10%; Reduce feed cost, it is stable to keep gastrointestinal bacterial flora, stops or alleviate the metabolic disease (like the cud acid poisoning) of milk cow; The disease of digestive tract (as have loose bowels with constipation etc.) of control milk cow; Improve the resistance against diseases of milk cow, the subclinical mastitis of control milk cow is saved medical fee; Also can improve the milk cow raising and oestrus and conception rate, increase the production performance of milk cow, the output of milk can improve 7.4-12.3%, and can reduce the body cell in the milk, improves the quality of milk, and butterfat percnetage improves about 14%.
Description of drawings
Fig. 1 prepares the technological process of compound microorganism ferments soybean skin feed.
The specific embodiment
Embodiment 1
The raw material ratio of compound microorganism ferments soybean skin feed: 800 kilograms of soybean skin; 200 kilograms of dregs of beans; Molasses 10-20 kilogram, and saccharomycete (content is: saccharomycete >=200,000,000/gram) 2-2.5 kilogram, and (content is lactic acid bacteria: lactic acid bacteria >=300,000,000/gram) 1-3 kilogram; (content is: bacillus >=300,000,000/gram) 1.5-3.5 kilogram, (content is Bifidobacterium bacillus: Bifidobacterium >=200,000,000/gram) 2-3 kilogram.
Embodiment 2
The method for preparing compound microorganism ferments soybean skin feed: (1) is placed on molasses 10-20 kilogram in about about 500 kilograms stainless steel cask; Adding 45 ℃ of warm water evenly dissolves and stirs and be blended into saccharomycete 2-2.5 kilogram after 30 minutes; Lactic acid bacteria 1-3 kilogram, bacillus 1.5-3.5 kilogram, Bifidobacterium 2-3 kilogram; Evenly stir after 30-50 minute sealing place 48 hours for use; The temperature of stainless steel cask should keep processing mixed bacteria liquid between 25-45 ℃ during this time. and (2) with 200 kilograms of dregs of beans, 800 kilograms of skin of beancurd homogeneous powders are broken to the 40-80 order and process dregs of rice skin mixture; (3) mixed bacteria liquid evenly is sprayed in the dregs of rice skin mixture, moisture Control is about 45-55%, and the biological feedstuff packaging bag of packing into, is put into the greenhouse and fermented by 25 or 40 kilograms every bag.(4) fermentation temperature was controlled at 20-30 ℃ in the 1st day to the 4th day, was controlled at about 45 ℃ in the 5th day to the 8th day, was controlled at about 25 ℃ in the 9th day to the 10th day.Pack into after will fermenting in (5) the 10th days the finished product detection metering successful and put in storage in the polybag of sealing.
Embodiment 3
Adopt the soy meal ground feed to replace above-mentioned soybean skin, preparation compound microorganism ferments soybean skin feed.The soy meal ground feed is made up of the processed soybean powder and the particle of soybean skin and pulverizer afterbody.
Embodiment 4
Adopt soybean skin to pulverize waste material and replace soybean skin, prepare compound microorganism ferments soybean skin feed.
Embodiment 5
Adopt soybean skin feed feeding milk cow of the present invention to experimentize: (one) experimental period: 18 days-June 18 May in 2010; (2) experiment place: pasture, Tai'an, Shandong; (3) experiment material: 6 tons in compound microorganism ferments soybean skin feed; (4) experiment method:, cut 1 kilogram of cow concentrate supplement simultaneously to every cow head 1 kilogram of this soybean skin feed of feeding every day; (5) an experiment number: 50 of the cows in milk of (1) experimental group.(2) 50 of the cows in milk of control group.(6) requirement of experiment: test milk bovine somatic cells, the test output of milk.
Output of milk testing result: on June 18th, 2010 measured 50 of control groups, and average per unit area yield is 15.5kg, and on June 18th, 2010 measured 50 of experimental group, and average per unit area yield is 17.35kg.
Table 1: somatic number testing result
After experiment was carried out 10 days, the ight soil stink of milk cow alleviated, and stench flavor is basic to disappear, and defecation is become thick by original spurting, the normal basically stercoroma round in heaps of ight soil after 15 days; After experiment is carried out 23 days, suckling, cattle eat grass speeds up, and forage is eaten to such an extent that totally remain material, and dietary amount increases; After experiment was carried out 25 days, the state of mind of milk cow was shiny black eagle-eyed by the original listless eyes that become, and becomes health status from sub-health state.
The summary experimental result: feeding the application's compound microorganism ferments soybean skin feed, negative conversion rate can reach 84.1%, and the output of milk on average increases 1.85kg, and somatic number drops to 22.1 ten thousand of June 18 from 78.1 ten thousand of May 20, descends 560,000.
Claims (4)
1. compound microorganism ferments soybean skin feed; It is characterized in that proportioning raw materials is: 800 kilograms of soybean skin, 200 kilograms of dregs of beans, molasses 10-20 kilogram; (content is saccharomycete: saccharomycete >=200,000,000/gram) 2-2.5 kilogram; (content is: lactic acid bacteria >=300,000,000/gram) 1-3 kilogram, (content is: bacillus >=300,000,000/gram) 1.5-3.5 kilogram, (content is Bifidobacterium bacillus lactic acid bacteria: Bifidobacterium >=200,000,000/gram) 2-3 kilogram.
2. compound microorganism ferments soybean skin feed as claimed in claim 1 is characterized in that adopting the soy meal ground feed to replace the soybean skin in the raw material, and this soy meal ground feed is made up of the processed soybean powder and the particle of soybean skin and pulverizer afterbody.
3. compound microorganism ferments soybean skin feed as claimed in claim 1 is characterized in that adopting soybean skin to pulverize waste material the soybean skin in the raw material replaces.
4. like the preparation method of claim 1,2 or 3 compound microorganism ferments soybean skin feed; It is characterized in that concrete steps are: (1) is placed on molasses 10-20 kilogram in about about 500 kilograms stainless steel cask; Adding 45 ℃ of warm water evenly dissolves and stirs and be blended into saccharomycete 2-2.5 kilogram, lactic acid bacteria 1-3 kilogram, bacillus 1.5-3.5 kilogram, Bifidobacterium 2-3 kilogram after 30 minutes; Evenly stir after 30-50 minute sealing place 48 hours for use; The temperature of stainless steel cask should keep processing mixed bacteria liquid between 25-45 ℃ during this time; (2) 200 kilograms of dregs of beans, 800 kilograms of skin of beancurd homogeneous powders are broken to the 40-80 order and process dregs of rice skin mixture; (3) mixed bacteria liquid evenly is sprayed in the dregs of rice skin mixture, moisture Control is about 45-55%, and the biological feedstuff packaging bag of packing into, is put into the greenhouse and fermented by 25 or 40 kilograms every bag; (4) fermentation temperature was controlled at 20-30 ℃ in the 1st day to the 4th day, was controlled at about 45 ℃ in the 5th day to the 8th day, was controlled at about 25 ℃ in the 9th day to the 10th day; Pack into after will fermenting in (5) the 10th days the finished product detection metering successful and put in storage in the polybag of sealing.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392920A (en) * | 2013-08-19 | 2013-11-20 | 青岛农业大学 | Fermentation method of soybean hulls |
| CN103409352A (en) * | 2013-08-19 | 2013-11-27 | 青岛农业大学 | Compound microbial community for preparing feed by fermenting soybean hulls |
| CN104982376A (en) * | 2015-06-08 | 2015-10-21 | 江苏省农业科学院 | Method of increasing daily diet digestible cellulose content for improving lamb meat quality |
| CN106036044A (en) * | 2016-05-23 | 2016-10-26 | 武威市顶乐生态牧业有限公司 | Alternative feed using agricultural product processing by-products as raw materials and production technology of alternative feed |
| CN109938179A (en) * | 2019-04-01 | 2019-06-28 | 华南农业大学 | It is a kind of to improve the gentle fermented feed for solving heat stress of standby milk cow growth performance and its preparation and application |
| CN110178986A (en) * | 2019-05-27 | 2019-08-30 | 广东通威饲料有限公司 | A kind of bean dregs are the preparation method of the biological feedstuff of raw material |
| CN110558420A (en) * | 2019-10-10 | 2019-12-13 | 北京挑战农业科技有限公司 | High-dietary-fiber low-antigen-protein fermented soybean hull |
| CN114391602A (en) * | 2022-01-20 | 2022-04-26 | 广西金陵农牧集团有限公司 | Biological fermentation feed taking weak-panning chicks as raw materials and preparation process and application thereof |
| CN115462443A (en) * | 2022-10-08 | 2022-12-13 | 山东香驰粮油有限公司 | Microbial fermentation feed and preparation method thereof |
| CN115736113A (en) * | 2022-11-18 | 2023-03-07 | 江苏汇福油脂科技有限公司 | Formula and manufacturing method of high-yield dairy cow feed produced by soybean hulls |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392920A (en) * | 2013-08-19 | 2013-11-20 | 青岛农业大学 | Fermentation method of soybean hulls |
| CN103409352A (en) * | 2013-08-19 | 2013-11-27 | 青岛农业大学 | Compound microbial community for preparing feed by fermenting soybean hulls |
| CN104982376A (en) * | 2015-06-08 | 2015-10-21 | 江苏省农业科学院 | Method of increasing daily diet digestible cellulose content for improving lamb meat quality |
| CN106036044A (en) * | 2016-05-23 | 2016-10-26 | 武威市顶乐生态牧业有限公司 | Alternative feed using agricultural product processing by-products as raw materials and production technology of alternative feed |
| CN109938179A (en) * | 2019-04-01 | 2019-06-28 | 华南农业大学 | It is a kind of to improve the gentle fermented feed for solving heat stress of standby milk cow growth performance and its preparation and application |
| CN110178986A (en) * | 2019-05-27 | 2019-08-30 | 广东通威饲料有限公司 | A kind of bean dregs are the preparation method of the biological feedstuff of raw material |
| CN110558420A (en) * | 2019-10-10 | 2019-12-13 | 北京挑战农业科技有限公司 | High-dietary-fiber low-antigen-protein fermented soybean hull |
| CN114391602A (en) * | 2022-01-20 | 2022-04-26 | 广西金陵农牧集团有限公司 | Biological fermentation feed taking weak-panning chicks as raw materials and preparation process and application thereof |
| CN115462443A (en) * | 2022-10-08 | 2022-12-13 | 山东香驰粮油有限公司 | Microbial fermentation feed and preparation method thereof |
| CN115736113A (en) * | 2022-11-18 | 2023-03-07 | 江苏汇福油脂科技有限公司 | Formula and manufacturing method of high-yield dairy cow feed produced by soybean hulls |
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Application publication date: 20120704 |