CN102524510A - Preparation method of low-fluorine Euphasia superb protein base stock - Google Patents
Preparation method of low-fluorine Euphasia superb protein base stock Download PDFInfo
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Abstract
The invention discloses a preparation method of low-fluorine Euphasia superb protein base stock. The preparation method comprises the following steps of: 1, low-temperature autolysis: homogenizing fresh and alive Euphasia superb and treating through ultraviolet irradiation to realize autolysis; 2, protein extraction: adding water into homogenate, stirring and extracting, carrying out centrifugal separation to obtain an upper-layer liquid phase and precipitates; 3, protein stabilization: refrigerating the upper-layer liquid for storage or drying the upper-layer liquid before refrigerating the upper-layer liquid for storage; 4, protein preparation: thawing the refrigerated upper-layer liquid; adjusting pH value to 4.8-5.8 or heating for 10-30 minutes at 80-100 DEG C and then centrifuging to obtain precipitate products and carrying out spray drying to finally obtain the Euphasia superb protein base stock. The preparation method is simple to operate and is quite suitable for field operation on a fishing ship; through adoption of the preparation method, the protein recovery rate is up to 46%-51% and the fat recovery rate is up to 54%-60%; and in the prepared low-fluorine Euphasia superb protein base stock, the protein content is 70-78%/100g of dry base stock, the fat content is 25-31g/100g of dry base stock and the fluorine content is lower than 1.5 mcg./g of dry base stock.
Description
Technical field
The present invention relates to utilize krill to prepare the method for albumen base-material.
Background technology
Krill (Euphausia superba) is single one of living resources of planting maximum on the earth, and the estimated value of its standing crop is about 4～1,500,000,000 tons, and ripe shrimp annual production is 3～500,000,000 tons, but a year quantity of the catch can reach about 100,000,000 tons, form huge potential fishery resources.In recent years, along with the depletion gradually of worldwide traditional fishery resource, and the proposition of 200-nautical-mile exclusive economic zone, make krill resource huge in the antarctic waters receive the concern of some deep-sea fishing developed countries.China has also listed the krill resource in one of main exploitation kind of deep-sea fishing development from now on.
Containing 11.9% to 15.4% albumen (wet basis) in the krill, is one of human available maximum protein resource that it has been established that.Krill is described as human protein resource treasure-house, and this is that not only protein content is high owing to krill, and the nutritive value of protein is also high.Once with the scoring of amino acid comprehensive nutrient value ratio of krill, prawn, cow's milk and beef, krill got a mark of 100 as a result, beef 96 minutes, cow's milk 91 minutes, prawn 71 minutes in the World Health Organization.According to one's analysis, necessary 8 seed amino acids of human body all have in the krill, and account for more than 40% of protein content altogether.Therefore, krill can be used as human important high-quality animal protein resource and is used.
That the Antarctic Continent is located in is remote, krill needs long period transportation, preservation after fishing for.The fluorine that contains high concentration in the krill shell can pollute shrimp to the shrimp migration in preserving process, influence the security of shrimp protein product.Simultaneously, be rich in polyunsaturated fatty acid in the krill, be prone to corrupt rotten.Therefore, krill is fished for need be freezing rapidly, ultralow temperature is preserved original quality that could guarantee shrimp, and the transportation preservation cost that this has improved krill greatly becomes the bottleneck that restricts the krill resources development and utilization.Solve the problem of krill transportation, preservation difficulty, best bet be exactly after fishing for rapidly with krill processing with preparation albumen, but still lack preparation for processing on the ship of krill albumen at present.
Summary of the invention
The present invention aims to provide a kind of simple and easy method is processed krill albumen base-material immediately after krill is fished for method, has low fluorine characteristic, especially is fit to operate on the dredger, can technical support be provided for the development and use of krill.
In order to achieve the above object, the invention discloses a kind of preparation method of low fluorine krill albumen base-material, it is characterized in that, in turn include the following steps:
Step 1, low temperature self-dissolving: the fresh and alive krill that will fish for drains directly homogenate acquisition homogenate of back; With the ultraviolet treatment with irradiation of 0.5～1 meter of 20～40 watts of power, wavelength 200～300 nanometers, distance 5～10 minutes, afterwards said homogenate is placed 0～4 ℃ of following self-dissolving 0.1～1 hour.
Step 2, protein extraction: the water of adding and 1～4 times of volume in said homogenate, mixing, stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, centrifugation, time upper phase of winning precipitated with the first time.
Step 3, protein Preparation: with described first time upper phase through regulating pH value to 4.8～5.8; Perhaps 80～100 ℃ are heated centrifugation method after 10～30 minutes down; Get upper phase and the deposition second time for the second time, with precipitating the spray-dried Euphausia superba protein base materials that promptly obtains the said second time.
Under the optimal way, in step Step 2, when homogenate added water, the adding final concentration was 0.01%～0.03% calcium chloride, to promote the protein dissolving.In addition, before centrifugation, regulate pH value to 2～3.5 of homogenate among the step Step 2.
In order to improve the quality of Euphausia superba protein base materials, after deposition can further be purified for the second time, carry out spray-drying again.Specifically, in step Step 3, can add the water of 2～4 times of volumes in the deposition to the said second time; Mixing; Regulating the pH of mixed value is 4.8～5.8, centrifugalize for the third time upper phase with precipitate for the third time, the said deposition for the third time promptly gets Euphausia superba protein base materials after spray-dried.
Under the optimal way, among the step Step 3, can be on dredger, for the first time upper phase frozen in-15 to-25 ℃ or spray-dried after frozen in-15 to-25 ℃ of preservations behind the dry powder; Then be transported to operation room; Again with said frozen first time upper phase melt or said frozen dry powder be dissolved in the water of 2～4 times of volumes and revert to liquid state, as the upper phase completing steps the Step 3 and subsequent step first time among the above-mentioned steps Step 3.
In order to improve the output of Euphausia superba protein base materials, in step Step 2, to 0～4 ℃ of water of 2～4 times of volumes of the said deposition adding first time; Mix; Stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, and centrifugation gets the 4th upper phase and the 4th deposition; Mix said first time liquid phase and said the 4th liquid phase as the liquid phase of using among the step Step 3 first time.
In the above-mentioned steps, the pH value of regulating homogenate can be selected HCl or the NaOH of 1～6M for use." draining " purpose is that the water that fresh and alive krill adheres to is removed among the Step 1 in addition, drains back shrimp itself and still keeps bright shrimp state.
Whole process of preparation of the present invention is all accomplished down at 0～4 ℃; This temperature is the antarctic average ambient temperature of catching season; Operating procedure all can aboard ship be accomplished; It is advantageous that the strong characteristics of fresh and alive krill self-dissolving ability of having utilized, increase the autolysis of structural proteins, improve the yield of water-solubility protein.Fluorine mainly concentrates on the shrimp shell in the fresh and alive krill, and not to the shrimp migration, extract with water this moment as yet, and fluorine mainly is distributed in the deposition, and is lighter to the pollution of water-soluble crude protein.In addition, the spray-dried back transportation of water-soluble krill crude protein increases transport capacity, practices thrift cost of transportation.The present invention has the following advantages:
1, the operating process that the present invention relates to is simple, does not need complex apparatus, and whole process of preparation is all accomplished down at 0～4 ℃, and this temperature is the antarctic average ambient temperature of catching season, is adapted at the enterprising line operate of dredger.
2, the present invention utilizes the strong characteristics of fresh and alive krill self-dissolving ability, increases the autolysis of structural proteins, improves the yield of water-solubility protein.
3, the fresh and alive krill that will fish for of the present invention drain the back directly homogenate operate; Because fluorine mainly concentrates on the shrimp shell in the fresh and alive krill, not to the shrimp migration, extract with water this moment as yet; Fluorine mainly is distributed in the deposition, has significantly reduced the pollution to water-soluble crude protein.
4, method of the present invention relates to freeze-drying of water-soluble krill crude protein or spray-dired step, has increased the transport capacity to the krill crude protein, practices thrift cost of transportation.
Method of the present invention is simple and effective: the crude protein rate of recovery that can make krill is 46%～51%, and the thick lipid rate of recovery is 54%～60%.The low-fluorine Euphausia superba protein base materials fluorine content of preparation is lower than 1.5ug/g; Be of high nutritive value, protein content is 70～78g/100g butt in the product, and fat content is 25～31g/100g butt, and content of phospholipid is 400～440mg/g; Purposes is wide, is fit to industrial applications, and the present invention can provide technical support for effective utilization of krill.
In addition, according to the technological parameter of the inventive method, can enhance productivity, product purity and quality are better simultaneously, have high economic benefit.
The specific embodiment
Disclosure of the Invention preparation for processing on a kind of ship of low fluorine krill albumen base-material, in turn include the following steps:
1, low temperature self-dissolving: the fresh and alive krill that will fish for drains directly homogenate of back; With the ultraviolet treatment with irradiation of 0.5～1 meter of 20～40 watts of power, wavelength 200～300 nanometers, distance 5～10 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.1～1 hour afterwards.This step has been utilized the strong characteristics of fresh and alive krill self-dissolving ability, increases the autolysis of structural proteins, improves the yield of water-solubility protein.
2, protein extraction: 0～4 ℃ of water of adding and 1～4 times of volume in the homogenate after the low temperature self-dissolving, mixing, stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, centrifugation, time upper phase of winning precipitated with the first time.Because fluorine mainly concentrates on the shrimp shell in the fresh and alive krill, not to the shrimp migration, extract with water this moment as yet, and fluorine mainly is distributed in the deposition, and is lighter to the pollution of water-soluble crude protein.
3, stabilize proteins: with the upper phase first time that obtains in the step 2 directly frozen or spray-dried after frozen behind the dry powder.This step has increased transport capacity, and practices thrift cost of transportation.
4, protein Preparation: the cryopreserving liquid thawing that obtains in the step 3 perhaps is dissolved in said frozen dry powder in the water of 2～4 times of volumes; Through regulating pH value to 4.8～5.8; Perhaps 80～100 ℃ of following heating centrifugation method after 10～30 minutes; Obtain deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
In the above-mentioned steps 2, when homogenate added water, the adding final concentration was 0.010%～0.030% calcium chloride.Through regulating pH value to 2～3.5 of homogenate, increase the albumen yield.
Prepare method of protein in the above-mentioned steps 4, different according to the product of step 3, following four kinds of situation are arranged specifically:
Method 1: after frozen liquid phase melted, regulating the pH value was 4.8～5.8, leaves standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate A; The water that in precipitate A, adds 2～4 times of volumes, stirring and evenly mixing, regulating the pH of mixed value is 4.8～5.8, leaves standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate B; Promptly get Euphausia superba protein base materials after precipitate B is spray-dried.
Method 2: after frozen liquid phase melted, 80～100 ℃ of heating 10～30 minutes were down left standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate A; Promptly get Euphausia superba protein base materials after precipitate A is spray-dried.
Method 3: frozen dry powder is dissolved in the water of 2～4 times of volumes, and regulating the pH value is 4.8～5.8, leaves standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate A; Add the water of 2～4 times of volumes to precipitate A, stirring and evenly mixing, regulating the pH of mixed value is 4.8～5.8, leaves standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate B; Promptly get Euphausia superba protein base materials after precipitate B is spray-dried.
Method 4: frozen dry powder is dissolved in the water of 2～4 times of volumes, and 80～100 ℃ of heating 10～30 minutes were down left standstill behind the stirring and evenly mixing 5～15 minutes, centrifugalize precipitate A; Promptly get Euphausia superba protein base materials after precipitate A is spray-dried.
Embodiment 1: the present invention is a raw material with the krill, adopts the extraction and separation technology of modern protein, has prepared processing and preparing technology on the ship of low-fluorine Euphausia superba protein base materials.Fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2～4 times of volumes; With the ultraviolet treatment with irradiation of 0.5～1 meter of 20～40 watts of power, wavelength 200～300 nanometers, distance 5～10 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.1～1 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.01%～0.03%, and the pH value of using 1～6M hydrochloric acid or NaOH to regulate homogenate is 2～3.5, and stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2～4 times of volumes to precipitate A; Mix; In mixed liquor, adding calcium chloride, to make its final concentration be 0.01% to 0.03%; The pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2～3.5, and stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Adding 1M hydrochloric acid or NaOH to liquid phase C, to regulate the pH value is 4.8～5.8, under 0～4 ℃ of condition, leaves standstill 5～15 minutes behind the stirring and evenly mixing, centrifugalize precipitate C; Add 0～4 ℃ water of 2～4 times of volumes to precipitate C, stirring and evenly mixing, in mixed liquor, adding 1M hydrochloric acid or NaOH, to regulate the pH value be 4.8～5.8, left standstill 5～15 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize deposit D; Promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 2: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2 times of volumes; With the ultraviolet treatment with irradiation of 1 meter of 40 watts of power, wavelength 200 nanometers, distance 10 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 1 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.03%, and the pH value of using 6M hydrochloric acid or NaOH to regulate homogenate is 3.5, and stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 4 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.03%, and the pH value of using 6M hydrochloric acid or NaOH to regulate mixed liquor is 3.5; Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Adding 6M hydrochloric acid or NaOH to liquid phase C, to regulate the pH value is 5.8, under 0～4 ℃ of condition, leaves standstill 15 minutes behind the stirring and evenly mixing, centrifugalize precipitate C; Add 0～4 ℃ water of 4 times of volumes to precipitate C, stirring and evenly mixing, in mixed liquor, adding 6M hydrochloric acid or NaOH, to regulate the pH value be 5.8, left standstill 15 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize deposit D; Promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 3: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2～4 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 30 watts of power, wavelength 250 nanometers, distance 7 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.5 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.02%, and the pH value of using 2M hydrochloric acid or NaOH to regulate homogenate is 3, and stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 3 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.02%, and the pH value of using 2M hydrochloric acid or NaOH to regulate mixed liquor is 3; Stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Adding 2M hydrochloric acid or NaOH to liquid phase C, to regulate the pH value is 5, under 0～4 ℃ of condition, leaves standstill 10 minutes behind the stirring and evenly mixing, centrifugalize precipitate C; Add 0～4 ℃ water of 3 times of volumes to precipitate C, stirring and evenly mixing, in mixed liquor, adding 2M hydrochloric acid or NaOH, to regulate the pH value be 5, left standstill 10 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize deposit D; Promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 4: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2 times of volumes; With the ultraviolet treatment with irradiation of 0.5 meter of 20 watts of power, wavelength 210 nanometers, distance 5 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.2 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.01%, and the pH value of using 1M hydrochloric acid or NaOH to regulate homogenate is 2, and stirring and leaching is 5 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Adding 1M hydrochloric acid or NaOH to liquid phase A, to regulate the pH value is 4.8, under 0～4 ℃ of condition, leaves standstill 5 minutes behind the stirring and evenly mixing, centrifugalize precipitate B; Add 0～4 ℃ water of 2 times of volumes to precipitate B, stirring and evenly mixing, in mixed liquor, adding 1M hydrochloric acid or NaOH, to regulate the pH value be 4.8, left standstill 5 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize precipitate C; Promptly get Euphausia superba protein base materials after precipitate C is spray-dried.
Embodiment 5: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 4 times of volumes; With the ultraviolet treatment with irradiation of 1 meter of 40 watts of power, wavelength 280 nanometers, distance 5 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.1 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.030%, and the pH value of using 4M hydrochloric acid or NaOH to regulate homogenate is 3.5, and stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Adding 1M hydrochloric acid or NaOH to liquid phase A, to regulate the pH value is 5.8, under 0～4 ℃ of condition, leaves standstill 15 minutes behind the stirring and evenly mixing, centrifugalize precipitate B; Add 0～4 ℃ water of 4 times of volumes to precipitate B, stirring and evenly mixing, in mixed liquor, adding 1M hydrochloric acid or NaOH, to regulate the pH value be 5.2, left standstill 15 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize precipitate C; Promptly get Euphausia superba protein base materials after precipitate C is spray-dried.
Embodiment 6: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 3 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 30 watts of power, wavelength 300 nanometers, distance 6 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.4 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.020%, and the pH value of using 2M hydrochloric acid or NaOH to regulate homogenate is 3, and stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Adding 1M hydrochloric acid or NaOH to liquid phase A, to regulate the pH value is 5, under 0～4 ℃ of condition, leaves standstill 10 minutes behind the stirring and evenly mixing, centrifugalize precipitate B; Add 0～4 ℃ water of 3 times of volumes to precipitate B, stirring and evenly mixing, in mixed liquor, adding 1M hydrochloric acid or NaOH, to regulate the pH value be 5, left standstill 10 minutes under 0～4 ℃ of condition behind the stirring and evenly mixing, centrifugalize precipitate C; Promptly get Euphausia superba protein base materials after precipitate C is spray-dried.
Embodiment 7: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2 times of volumes; With the ultraviolet treatment with irradiation of 0.6 meter of 25 watts of power, wavelength 220 nanometers, distance 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.7 hour afterwards.Stirring and leaching is 6 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Add 0～4 ℃ of water of 3 times of volumes to precipitate A, mix, stirring and leaching is 8 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-15 ℃ of preservations; Melt cryopreserving liquid, it is centrifugal to regulate pH value to 4.8 back, obtains deposition, will precipitate spray-drying and promptly get Euphausia superba protein base materials.
Embodiment 8: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 4 times of volumes; With the ultraviolet treatment with irradiation of 0.7 meter of 35 watts of power, wavelength 290 nanometers, distance 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.3 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.015%, and the pH value of using 1M hydrochloric acid or NaOH to regulate homogenate is 2.4, and stirring and leaching is 11 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.015%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2.5; Stirring and leaching is 11 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-20 ℃ of preservations; Melt cryopreserving liquid, it is centrifugal to regulate pH value to 5.2 back, obtains precipitate C, in precipitate C, adds the water of 2 times of volumes, stirring and evenly mixing, regulating the pH of mixed value is 5.2, centrifugalize liquid phase D and deposit D, promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 9: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 3 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 35 watts of power, wavelength 255 nanometers, distance 6 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.4 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.025%, and the pH value of using 2M hydrochloric acid or NaOH to regulate homogenate is 3, and stirring and leaching is 12 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2～4 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.025%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2; Stirring and leaching is 12 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-25 ℃ of preservations; Melt cryopreserving liquid, it is centrifugal to regulate pH value to 5 back, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
Embodiment 10: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2.5 times of volumes; With the ultraviolet treatment with irradiation of 0.9 meter of 28 watts of power, wavelength 230 nanometers, distance 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.5 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 3M hydrochloric acid or NaOH to regulate homogenate is 2.5, and stirring and leaching is 5 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 3 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2.5; Stirring and leaching is 5 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-22 ℃ of preservations; Melt cryopreserving liquid, it is centrifugal to regulate pH value to 5.5 back, obtains precipitate C, in precipitate C, adds the water of 4 times of volumes, stirring and evenly mixing, regulating the pH of mixed value is 5.5, centrifugalize liquid phase D and deposit D, promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 11: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 3.5 times of volumes; With the ultraviolet treatment with irradiation of 1 meter of 36 watts of power, wavelength 205 nanometers, distance 9 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.6 hour afterwards.Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Add 0～4 ℃ of water of 2 times of volumes to precipitate A, mix, stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Obtain liquid phase C; With liquid phase C after spray-dried frozen in-21 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 2 times of volumes, and it is centrifugal to regulate pH value to 4.9 back, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
Embodiment 12: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2～4 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 30 watts of power, wavelength 245 nanometers, distance 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.7 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.030%, and the pH value of using 4M hydrochloric acid or NaOH to regulate homogenate is 3, and stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 4 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.030%, and the pH value of using 4M hydrochloric acid or NaOH to regulate mixed liquor is 3; Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; With liquid phase C after spray-dried frozen in-18 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 4 times of volumes; It is centrifugal to regulate pH value to 5.1 back, obtains precipitate C, in precipitate C, adds the water of 4 times of volumes; Stirring and evenly mixing; Regulating the pH of mixed value is 5.1, centrifugalize liquid phase D and deposit D, promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 13: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 3 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 30 watts of power, wavelength 265 nanometers, distance 6 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.8 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.030%, and the pH value of using 5M hydrochloric acid or NaOH to regulate homogenate is 3, and stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 5M hydrochloric acid or NaOH to regulate mixed liquor is 3; Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; With liquid phase C after spray-dried frozen in-24 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 2 times of volumes, and it is centrifugal to regulate pH value to 5.0 back, obtains deposition, will precipitate spray-drying and promptly get Euphausia superba protein base materials.
Embodiment 14: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 4 times of volumes; With the ultraviolet treatment with irradiation of 0.5 meter of 40 watts of power, wavelength 260 nanometers, distance 9 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.9 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.025%, and the pH value of using 1M hydrochloric acid or NaOH to regulate homogenate is 2.5, and stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 4 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2; Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; With liquid phase C after spray-dried frozen in-16 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 2～4 times of volumes; It is centrifugal to regulate pH value to 5.8 back, obtains precipitate C, in precipitate C, adds the water of 2 times of volumes; Stirring and evenly mixing; Regulating the pH of mixed value is 5.0, centrifugalize liquid phase D and deposit D, promptly get Euphausia superba protein base materials after deposit D is spray-dried.
Embodiment 15: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2 times of volumes; With the ultraviolet treatment with irradiation of 0.5 meter of 30 watts of power, wavelength 275 nanometers, distance 9 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.2 hour afterwards.Stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Add 0～4 ℃ of water of 2 times of volumes to precipitate A, mix, stirring and leaching is 5 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-20 ℃ of preservations; Melt cryopreserving liquid, 100 ℃ heating is centrifugal after 10 minutes down, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
Embodiment 16: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 3 times of volumes; With the ultraviolet treatment with irradiation of 0.8 meter of 30 watts of power, wavelength 285 nanometers, distance 9 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.3 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate homogenate is 2.5, and stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 2.5; Stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Liquid phase C is frozen in-19 ℃ of preservations; Melt cryopreserving liquid, 80 ℃ heating is centrifugal after 30 minutes down, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
Embodiment 17: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 2 times of volumes; With the ultraviolet treatment with irradiation of 0.5 meter of 20 watts of power, wavelength 295 nanometers, distance 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.6 hour afterwards.Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; Add 0～4 ℃ of water of 2 times of volumes to precipitate A, mix, stirring and leaching is 10 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; Obtain liquid phase C; With liquid phase C after spray-dried frozen in-21 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 2 times of volumes, and 90 ℃ heating is centrifugal after 20 minutes down, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
Embodiment 18: the fresh and alive krill after fishing for drains away the water; Mix back homogenate with 0～4 ℃ of water of 4 times of volumes; With the ultraviolet treatment with irradiation of 40 watts of power, wavelength 225 nanometers, distance 0.5 8 minutes, homogenate is placed fished under the area surroundings temperature self-dissolving 0.2 hour afterwards.In homogenate, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate homogenate is 3.5, and stirring and leaching is 5 minutes under 0～4 ℃ of condition, centrifugalize liquid phase A and precipitate A; 0～4 ℃ of water that adds 2 times of volumes to precipitate A; Mix, in mixed liquor, adding calcium chloride, to make its final concentration be 0.010%, and the pH value of using 1M hydrochloric acid or NaOH to regulate mixed liquor is 3.5; Stirring and leaching is 15 minutes under 0～4 ℃ of condition, centrifugalize liquid phase B and precipitate B; Merge liquid phase A and liquid phase B, obtain liquid phase C; With liquid phase C after spray-dried frozen in-23 ℃ of preservations behind the dry powder; Said frozen dry powder is dissolved in the water of 2 times of volumes, and 85 ℃ heating is centrifugal after 30 minutes down, obtains deposition, the spray-dried Euphausia superba protein base materials that promptly gets.
The above; Be merely the preferable specific embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any technical staff who is familiar with the present technique field is equal to replacement or change according to technical scheme of the present invention and inventive concept thereof in the technical scope that the present invention discloses, all should be encompassed within protection scope of the present invention.
Claims (7)
1. the preparation method of one kind low fluorine krill albumen base-material is characterized in that, in turn includes the following steps:
S1, low temperature self-dissolving: the fresh and alive krill that will fish for drains back homogenate and obtains homogenate; With the ultraviolet treatment with irradiation of 0.5～1 meter of 20～40 watts of power, wavelength 200～300 nanometers, distance 5～10 minutes, afterwards said homogenate is placed 0～4 ℃ of following self-dissolving 0.1～1 hour;
S2, protein extraction: in said homogenate, add the water of 1～4 times of volume, mixing, stirring and leaching is 5～15 minutes under 0～4 ℃ of condition, centrifugation, time upper phase of winning and the deposition first time;
S3, protein Preparation: with described first time upper phase through regulating ℃ down heating 10～30 minutes of pH value to 4.8～5.8 or 80～100; Then centrifugation method gets upper phase and the deposition second time for the second time, with the spray-dried Euphausia superba protein base materials that promptly obtains of the said deposition second time.
2. the preparation method of low fluorine krill albumen base-material as claimed in claim 1 is characterized in that: in step S2, when homogenate added water, the adding final concentration was 0.01%～0.03% calcium chloride.
3. the preparation method of low fluorine krill albumen base-material as claimed in claim 2 is characterized in that: pH value to 2～3.5 of in step S2, before centrifugation, regulating said homogenate.
4. the preparation method of low fluorine krill albumen base-material as claimed in claim 3; It is characterized in that: in step S3; The water that in precipitating the said second time, adds 2～4 times of volumes, mixing, regulating the pH of mixed value is 4.8～5.8; Centrifugalize upper phase and deposition for the third time for the third time, the said deposition for the third time promptly gets Euphausia superba protein base materials after spray-dried.
5. the preparation method of low fluorine krill albumen base-material as claimed in claim 4 is characterized in that: in step S3, with said first time upper phase frozen in-15 to-25 ℃ or spray-dried after frozen in-15 to-25 ℃ of preservations behind the dry powder; After then being transported to operation room, with said frozen first time upper phase melt or said frozen dry powder be dissolved in the water of 2～4 times of volumes and revert to liquid state, completing steps S3 and subsequent step.
6. like the preparation method of the arbitrary described low fluorine krill albumen base-material of claim 1～5; It is characterized in that: among the step S2; 0～4 ℃ of water to 2～4 times of volumes of the said deposition adding first time mixes, and stirring and leaching is 5～15 minutes under 0～4 ℃ of condition; Centrifugation gets the 4th upper phase and the 4th deposition; Mix said first time liquid phase and said the 4th liquid phase as the liquid phase of using among the step S3 first time.
7. like the preparation method of the arbitrary described low fluorine krill albumen base-material of claim 1～5, it is characterized in that: the pH value of regulating said homogenate is selected HCl or the NaOH of 1～6M for use.
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| CN105433293A (en) * | 2015-12-07 | 2016-03-30 | 中国海洋大学 | Minced euphausia superba rich in phospholipid and preparing method thereof |
| CN105454403A (en) * | 2015-12-07 | 2016-04-06 | 中国海洋大学 | Low-flourine frozen Antarctic krill mince and preparation method thereof |
| CN105433293B (en) * | 2015-12-07 | 2017-04-05 | 中国海洋大学 | The krill shrimp gruel of enrichment phosphatide and its production method |
| CN107125492A (en) * | 2017-04-28 | 2017-09-05 | 广东越群海洋生物研究开发有限公司 | Application of the euphausia superba powder in terms of madai opening material is prepared |
| CN110229225A (en) * | 2019-06-12 | 2019-09-13 | 山东师范大学 | The preparation method of sulfur-rich and/or rich caesium albumen in a kind of krill |
| CN110229225B (en) * | 2019-06-12 | 2021-07-20 | 山东师范大学 | Preparation method of sulfur-rich and/or cesium-rich protein in antarctic krill |
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Inventor after: Zhou Dayong Inventor after: Zhu Beiwei Inventor after: Yang Jingfeng Inventor after: Chen Yuewen Inventor after: Wu Haitao Inventor after: Li Dongmei Inventor after: Li Ming Inventor before: Zhu Beiwei Inventor before: Yang Jingfeng Inventor before: Chen Yuewen Inventor before: Wu Haitao Inventor before: Li Dongmei Inventor before: Li Ming |
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