CN102524188B - Insect breeding bottom and breeding method of gynaikothrips ficorum - Google Patents
Insect breeding bottom and breeding method of gynaikothrips ficorum Download PDFInfo
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Abstract
The invention relates to an insect breeding bottom and a breeding method of gynaikothrips ficorum and belongs to the field of insect breeding and insect ecology. A bottom body is in a cone frustum shape with large top and small bottom and is in sealing match with a bottle cover, a magnifying glass is installed at the center of the bottle cover, a plurality of venting holes are evenly distributed on the bottle cover, and a filter screen is installed on the venting holes. The method utilizing the insect breeding bottom to breed the gynaikothrips ficorum comprises (1) collection of eggs, (2) hatching of the eggs, (3) breeding of nymphs and (4) breeding of imagoes. By using the breeding method, the hatching rate, survival rate and eclosion rate of the gynaikothrips ficorum are high, massive thrips of various insect states which are orderly in age and high in vigor can be obtained, and form changes and the whole life history of the gynaikothrips ficorum can be observed in real time.
Description
Technical field
The present invention relates to the raising method of a breeding worm bottle and banyan pipe thrips, belong to insect and raise and the Entomological Ecology field.
Background technology
Banyan pipe thrips is that spire banyan, Ficus microcarpa, banyan, gas reach a kind of primary pest on the banyans such as banyan, gold banyan, host plant somebody face, fig, azalea, dragon-boat flower, mango, grey jasmine etc.With regard to regard to the causing harm of banyan, inhale banyan tender leaf, young shoot with adult, nymph file, first along tender leaf master pulse both sides file, inhale juice, form puce not of uniform size or brown spot at leaf back, and then along in arteries and veins folding to blade face, the serious deformity that causes, as blade face distortion or curling along master pulse, form the insect gall of dumpling shape or pimple shape, the insect gall appearance is covered with the bronzing spot; On a blade, generally have 3 to send residence, the harm of trooping of tens to up to a hundred nymphs, adults is inside arranged, the leaf of being injured is rolled into a pile, and greenery become buff, similarly are to be drooped in the heat of the sun; Cause harm and cause many circular small particles at old limb edge.Often with the banyan seedling, be injured heavier, especially on the potted landscape of making in banyan, be injured heavier, often cause the curling variable color of tender leaf, serious whole strain tender leaf all is curled into the dumpling shape, the banyan growth retardation of being injured, photosynthesis weakens, and has seriously affected the moulding work in art value, economic worth and the production of banyan potted landscape.This worm is a difficult problem in banyan Cultivate administration and banyan growth.In recent years, also find that banyan pipe thrips can sting the people, the countries such as Thailand, Japan, Slovakia, the U.S. classify this worm as Alien Invasive Species.
China is plantation and the big export country of banyan, and the banyan varieties of plant nearly more than 20 is planted, and the banyan potted landscape of outlet accounts for more than 90% of international market share.During nowadays some enterprises outlet potted landscape banyan, owing to carrying banyan pipe thrips, do not meet the export quarantine requirement, the disinfection of must quarantining before outlet, therefore, thereby study its history of life, morphological feature is made prevention and control measure becomes urgent task.The basis of these researchs be to have mass rearing, grow neat banyan pipe thrips.
In existing document, the report of indoor mass rearing banyan pipe thrips is not arranged, the raising method of some other thrips is only arranged, comprehensive, the raising method such as concave slide method for breeding, penicillin bottle method for breeding, potted plant host plant are also only arranged.The concave slide method for breeding was to be involutory together with two concave slides, places the blade with thrips within it, and two concave slides are fixed with rubber band, is positioned in porcelain dish, and porcelain dish is positioned in climatic cabinate and raises, and changed blade every 2-3 days.Palm thrips adopts this method to be raised.Concave slide method for breeding advantage is the little space that can form relative closure while relatively being closed by two concave slides, and thrips can't be escaped.Its shortcoming is that in little space, air almost can't circulate, and blade is breathed and caused humidity larger, is unfavorable for that thrips survives therein, and vigor and survival rate are lower.When changing blade, thrips easily takes advantage of the occasion to run away, and is unfavorable for operation.The penicillin bottle method for breeding is place mat thin layer absorbent cotton in the penicillin bottle, covers the filter paper with end diameter on absorbent cotton, adds suitable quantity of water, with 100 order 5cm * 5cm gauze net cover rubber band, seals bottleneck, prevents the thrips escape.Put into one, thrips ovum and fresh blade in every bottle, with the 2ml syringe, add suitable quantity of water on filter paper at the bottom of bottle, keep humidity in bottle.Seal bottleneck with the gauze net cover rubber band, put into the porcelain basin, raise in 25 ℃ of climatic cabinates, changed blade every 2-3 days.The activity space that the penicillin bottle method provides is large, and blade is easily fresh-keeping, and most nymph all can be bored in the cotton under people's filter paper and pupate.Penicillin bottle method advantage is that material is easy to get, and the survival rate of thrips is higher.Shortcoming is operation inconvenience, especially changes blade, seals the envelope of getting of gauze, and can only raise a small amount of insect population in each bottle.Potted plant host plant method for breeding is for the loti of feeding, and potted plant clover under without the worm environment, connect worm at branching stage and raise.This method advantage is to approach natural conditions, can obtain the loti that a large amount of vigor are high.Shortcoming is that loti belongs to the microminiature insect, catch difficulty, and generation overlap is serious, is difficult to obtain worm consistent polypide in age.For banyan pipe thrips, it grows needs Ficus microcarpa leaf, and Ficus microcarpa leaf need be rolled and can be laid eggs, and in concave slide method for breeding and penicillin bottle method for breeding, breeding facility is too small, complete Ficus microcarpa leaf can't be put into wherein; Host plant is raised rule because the banyan plant is larger, and required dependent insect cage is also large, and banyan pipe thrips is small due to build, can cause and catches difficulty and very easily escape.Therefore, above 3 kinds of methods all are not suitable for for the banyan pipe thrips of feeding.
Summary of the invention
The object of the present invention is to provide the raising method of a breeding worm bottle and banyan pipe thrips, to realize growing each neat, that vigor is higher worm state banyan pipe thrips in indoor acquisition in enormous quantities.
The invention provides a breeding worm bottle, formed the taper type that described body is big up and small down by body and bottle cap, described body and bottle cap sealing coordinate, the central authorities of described bottle cap are equipped with magnifying glass, are evenly distributed with a plurality of ventilation holes on described bottle cap, and described ventilation hole is equipped with screen pack.
Described body and bottle cap are made by transparent lucite.
The footpath suitable for reading diameter of described body be 98mm, end opening footpath diameter be 90mm, highly for 98mm.
The opening part of described body is provided with the sealing ground, and the height of sealing ground is 5mm; The edge of described bottle cap is provided with and the annular protrusion that seals ground and match, and the thickness of described annular protrusion is 2.5mm, highly is 15mm.
The multiplication factor of described magnifying glass is 3 times, and diameter is 80mm.
The quantity of described ventilation hole is 8, and the ventilation hole diameter is 5mm.
Described screen pack is the above screen packs of 80 orders.
The present invention also provides a kind of and has utilized described foster worm bottle to raise the method for banyan pipe thrips, said method comprising the steps of:
(1) collection of ovum: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather the banyan branch, 2～3 blades must be arranged on branch and at least will have an insect gall to exist; The branch blade is cleaned out, after in the branch bottom coated cotton mass, suck hydromel on cotton mass; Branch is vertically put into and supported the worm bottle, the state and do not contact a bottle wall of being kept upright; Banyan pipe thrips adult is received on the branch blade, and in the foster worm bottle shift-in phytotron that is connected to banyan pipe thrips, adult automatically proceeded in branch insect gall blade and lays eggs in 3 days, and remove adult postpartum, collected the insect gall blade of laying eggs, and obtained relatively consistent ovum;
(2) hatching of ovum: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather banyan and newly take out then the 2nd or the 3rd leaf on spray top, blade is cleaned out, be immersed in 25-35 second in hydromel, take out the nature airing, after at the petiole place coated cotton mass, suck hydromel on cotton mass; The blade level is put into and supported the worm bottle, do not contact bottle wall and blade face upward, the insect gall blade of laying eggs of collecting is put into subsequently and supported the worm bottle, then rest in worm bottle shift-in phytotron and hatch; Ovum hatching be 1 age nymph, and be transferred to voluntarily by the insect gall blade of laying eggs on the blade of collection and take food and cause harm;
(3) nymph is raised: the insect gall blade of laying eggs that step (2) is supported in the worm bottle is taken away, changes to the 2nd or the 3rd leaf that banyan is newly taken out the spray top then, the same step of processing method (2); Need to change fresh blade in the nymphal development process, make each nymph be transferred to voluntarily and newly change in blade by old blade in age, finally develop into adult;
(4) imago breeding: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather banyan and newly take out then shoot, have 3 blades on branch at least, the same step of branch processing method (1); Several branches that gather are progressively put into and supported the worm bottle, and built, keep substantially upright mutually, guarantees that the blade between each branch overlaps; To develop on the same day the blade of feeding of adult, relay gently on the built be comprised of branch, adult can be transferred on the branch blade voluntarily, after the blade of feeding from supporting in the worm bottle, take away, rest in worm bottle shift-in phytotron and raise;
The controlled condition of the phytotron that above each step adopts is: 25 ± 1 ℃ of temperature, humidity 60% ± 5%, photoperiod L:D=8:l6h.
Above-mentioned for nymph can be shifted voluntarily between blade, two blades of supporting in the worm bottle need overlap.
Described hydromel is the hydromel of 200 times of dilutions.
The thickness of described colored mud is 5mm, water suction 6-8ml.
Use raising method of the present invention, banyan pipe thrips incubation rate, survival rate, eclosion rate are high, can obtain each worm state thrips that the length of time in enormous quantities is neat, vigor is higher, but and metamorphosis and the whole history of life of real-time monitored banyan pipe thrips.
the present invention has following effect:
1, can meet banyan pipe thrips demand of raising in enormous quantities, in indoor acquisition, grow each neat, that vigor is higher worm state banyan pipe thrips.Banyan pipe thrips is according to said method raised, and the egg laying amount of female adult is at 50～70 intergranulars, and the incubation rate of ovum is more than 83.33%; Nymph was divided into for 4 ages, and the time that each instar larvae occurs and the length of time are all stable and more neat, and the nymph eclosion rate, up to more than 90.00%, can obtain the banyan pipe thrips adult of high vigor more than 200 in each foster worm bottle; The adult of raising can continue to keep high vigor, and growth is very neat, not there will be the death of adult.
2, can meet the demand of banyan pipe thrips breeding observing, in metamorphosis and the whole history of life of indoor real-time monitored banyan pipe thrips.Each, nymph and adult had light green property in age, all can be transferred to voluntarily on the spray blade of new replacing; Adult has stronger phototeletaxis, often is clustered in the blade shady face; Ovum through about 7 days, hatch be 1 age nymph, 1 age nymph cephalothorax accounting large, merogenesis is not obvious, taking food caused harm about 3 days develop into 2 age nymph; 2 age the nymph merogenesis obvious, there is no external wing bud, taking food caused harm about 8 days develop into 3 age nymph; 3 age the nymph feeler become very short cryptomere projection, grow external wing bud, movable blunt, about 3 days, develop into 4 age nymph; Nymph feeler elongation in 4 ages and curved behind to head, sheath shape wing bud is stretched and is reached belly 3/5 place, and activity is very blunt, about 5 days, develops into adult.In addition, can also understand the mechanics of adult, the situation that particularly causes harm and mating process.
3, special foster worm bottle is raised banyan pipe thrips with observation and is combined, and has two large functions: the one, and can meet banyan pipe thrips and raise needed space and environment condition, ventilative and logical light; The 2nd, can meet the variation of real-time monitored banyan pipe thrips in whole growth and development process.
4, the present invention is easy and simple to handle, takes full advantage of the moisture-keeping functions of colored mud, in foster worm bottle bottom, puts into colored mud and filter paper, adds a water and just can meet banyan pipe thrips raising demand, without any mildew phenomena, produces, and successful is better than with absorbent cotton or sponge; Take full advantage of the light green property of becoming of banyan pipe thrips and phototeletaxis, realize thrips adult, the right transfer voluntarily of newly changing branches and leaves of nymph, without artificial migration polypide, avoid damage and death to thrips; Take full advantage of the fresh-keeping nutritious effect of hydromel, the blade of feeding carries out the short time immersion with the dilution hydromel, and is coated with at the petiole place cotton mass that sucks the dilution hydromel, has promoted the duration of Leaf Vitality, has reduced the blade exchanging frequency.
The accompanying drawing explanation
Fig. 1 is for supporting the structural map of worm bottle.
Embodiment
example example one: the batch of banyan pipe thrips is raised
(1) raising condition is explored
1, the selection of moisturizing medium
test material:special foster worm bottle, absorbent cotton, sponge, flower mud, filter paper, banyan blade, clear water, banyan pipe thrips adult.
test method:test is established 3 groups and is processed 3 repetitions.Process corresponding the putting into and sponge of the same size, absorbent cotton, flower mud at the bottom of bottle in the bottom of supporting the worm bottle for every group, thickness is 5mm, after its water suction 8ml, puts the onesize filter paper of a slice thereon; Gather banyan and newly take out then the 3rd leaf on spray top, with hooking the line writing brush, blade is cleaned out, after cotton mass after the coated water suction in petiole place, the blade level is put into to foster worm bottle, do not contact bottle wall and blade face upward; Every bottle with hooking 10 banyan pipe thrips adults of line writing brush access, male and female are than being 3:2, after be positioned over (26 ± 1 ℃ of temperature, humidity 60% ± 5%, photoperiod L:D=10:14h) in climatic cabinate, continuous 7 days, by 3 times of active situation and fresh-keeping situations of blade that magnifying glass is regularly observed banyan pipe thrips adult of bottle cap central authorities, if any adult death, open bottle cap every day, confirmed with hooking line writing brush shake-up adult, finally compared the fresh-keeping situation of activity, death and blade of banyan pipe thrips adult.
result of the test:the statistics of 7 days is in Table 1.Known: 3 kinds of moisturizing media can not cause too large impact to the activity of banyan pipe thrips adult, and the banyan pipe thrips adult on flower mud or sponge is more active, have the mate and oviposit phenomenon to occur; Banyan pipe thrips adult on sponge or absorbent cotton occurs dead on the 3rd day, and a little earlier in flower mud, the general mortality rate of last adult is also all higher than 6.67 percentage points, flower mud; Banyan blade on absorbent cotton all occurs that variable color and petiole come off, and the blade-section on sponge occurs that variable color and petiole come off, and the blade on flower mud just slightly has variable color at the petiole place.To sum up result shows with flower mud best as the effect of moisturizing medium.
table 1 moisturizing medium is to the activity of banyan pipe thrips adult, death and the fresh-keeping situation of blade
2, spend the selection of mud water absorption
test material:special foster worm bottle, flower mud, filter paper, banyan blade, absorbent cotton, clear water, banyan pipe thrips adult.
test method:test is established 3 groups and is processed 3 repetitions.Process corresponding the putting into and colored mud of the same size at the bottom of bottle in bottom of supporting the worm bottle for every group, thickness 5mm, after it absorbs water respectively 6ml, 8ml and 10ml, put the onesize filter paper of a slice thereon; Successor operation and observational technique are with the selection of moisturizing medium
.
result of the test:the statistics of 7 days is in Table 2.Known: the banyan pipe thrips adult on water suction 10ml flower mud owes active, and the amixia phenomenon of laying eggs occurs; Though the banyan pipe thrips adult on water suction 8ml or 6ml flower mud is mate and oviposit all, liveness is slightly more strong with water suction 8ml's, and it is also more some more to lay eggs.Banyan pipe thrips adult on water suction 10ml flower mud occurs dead on the 3rd day, and in other 2 groups, general mortality rate is up to 86.67% a little earlier; Banyan pipe thrips adult on water suction 6ml or 8ml flower mud all occurred dead at the 4th day, what but general mortality rate absorbs water, 8ml came is low.Banyan blade-section on water suction 6ml or 10ml flower mud occurs that variable color and petiole come off, and the blade on water suction 8ml flower mud just slightly has variable color at the petiole place.To sum up result shows the best results of flower mud water suction with 8ml, and 6ml takes second place.
table 2 flower mud water absorption is to the activity of banyan pipe thrips adult, death and the fresh-keeping situation of blade
3, the selection of the fresh-keeping mode of blade
test material:special foster worm bottle, flower mud, filter paper, banyan blade, absorbent cotton, hydromel, clear water, banyan pipe thrips adult.
test method:test is established 4 groups and is processed 3 repetitions.Process corresponding the putting into and colored mud of the same size at the bottom of bottle in bottom of supporting the worm bottle for every group, thickness 5mm, after it absorbs water respectively 8ml, put the onesize filter paper of a slice thereon; Gather banyan and newly take out then the 3rd leaf on spray top, with hooking the line writing brush, blade is cleaned out, a half vane is soaked 30 seconds in 200 times of hydromels of dilution, take out the nature airing; Blade is carried out to Preservation Treatment by following 4 kinds of modes: blade+petiole clear water cotton mass, blade+petiole hydromel cotton mass, sticky mulse blade+petiole clear water cotton mass, sticky mulse blade+petiole hydromel cotton mass; Successor operation and observational technique are with the selection of moisturizing medium
.
result of the test:the statistics of 7 days is in Table 3.Known: the banyan pipe thrips adult of 4 kinds of fresh-keeping modes of blade all has mate and oviposit, but 3 kinds of having hydromel to process are more active, lay eggs also more some more.The fresh-keeping mode that does not have any hydromel to process, banyan pipe thrips adult occurs dead on the 4th day, a little earlier in other 3 kinds, general mortality rate also, apparently higher than other 3 kinds, reaches 46.67%; 3 kinds of fresh-keeping modes of blade that have hydromel to process, banyan pipe thrips adult the 5th talent occurs dead, general mortality rate is all lower, to glue the minimum of mulse blade+petiole hydromel cotton mass, is only 6.67%.The fresh-keeping mode that does not have any hydromel to process, blade is without variable color, but slightly variable color of petiole; 3 kinds of fresh-keeping modes of blade that have hydromel to process, blade, petiole are all without variable color.To sum up result shows that sticky mulse blade+petiole hydromel cotton mass processing is best to the fresh-keeping effect of blade.
the fresh-keeping mode of table 3 blade is to the activity of banyan pipe thrips adult, death and the fresh-keeping situation of blade
4, the feed selection of blade
test material:special foster worm bottle, flower mud, filter paper, banyan blade, absorbent cotton, hydromel, banyan pipe thrips adult.
test method:test is established 5 groups and is processed 3 repetitions.Process corresponding the putting into and colored mud of the same size at the bottom of bottle in bottom of supporting the worm bottle for every group, thickness 5mm, after it absorbs water respectively 8ml, put the onesize filter paper of a slice thereon; Gather banyan and newly take out then the 1st to the 5th leaf on spray top, be divided into 5 groups, every group of blade all cleaned out blade with hooking the line writing brush, in 200 times of hydromels of dilution, soak 30 seconds, take out the nature airing, after at the petiole place the coated cotton mass that sucks 200 times of hydromels of dilution, the blade level is put into and is supported the worm bottle, do not contact bottle wall and blade face upward; Successor operation and observational technique are with the selection of moisturizing medium
.
result of the test:the statistics of 7 days is in Table 4.Known: banyan pipe thrips adult is active on the 1st to the 4th leaf, and mate and oviposit is arranged, and the 1st leaf laid eggs at most, and it is minimum that the 4th leaf laid eggs, and to the 5th leaf amixia, lays eggs.Banyan pipe thrips adult occurs dead on the 4th day at the 1st leaf, early than other blade, general mortality rate is up to 80.00%; In the general mortality rate of the 4th, 5 leaves more than 53.33%, apparently higher than the 2nd, 3 leaves.The 1st leaf blade variable color is withered, and the petiole brown stain comes off; The the 4th and the 5th leaf all has the partial blade variable color, and petiole also all has a small amount of variable color; 2nd, 3 blades and petiole are substantially without variable color, and at most just annesl a little appears in petiole.To sum up result shows with the feed effect of banyan pipe thrips of the 2nd leaf or the 3rd leaf best.
table 4 is fed blade to the activity of banyan pipe thrips adult, death and the fresh-keeping situation of blade
5, the selection of light application time
test material:special foster worm bottle, flower mud, filter paper, banyan blade, absorbent cotton, hydromel, banyan pipe thrips adult.
test method:test is established 3 groups and is processed 3 repetitions.Process corresponding the putting into and colored mud of the same size at the bottom of bottle in bottom of supporting the worm bottle for every group, thickness 5mm, after it absorbs water respectively 8ml, put the onesize filter paper of a slice thereon; Gather banyan and newly take out then the 3rd leaf on spray top, with hooking the line writing brush, blade is cleaned out, in 200 times of hydromels of dilution, soak 30 seconds, take out the nature airing, after at the petiole place the coated cotton mass that sucks 200 times of hydromels of dilution, the blade level is put into and supported the worm bottle, do not contact bottle wall and blade face upward; Every bottle with hooking 10 banyan pipe thrips adults of line writing brush access, and male and female are than being 3:2, after be positioned in climatic cabinate, climatic cabinate is divided into 3 groups, temperature is 26 ± 1 ℃, humidity is 60% ± 5%, the photoperiod is arranged to respectively 10:14h, 8:16h, 6:18h.Successor operation and observational technique are with the selection of moisturizing medium
.
result of the test:the statistics of 7 days is in Table 5.Known: banyan pipe thrips adult all has mate and oviposit at 10:14h or 8:16h under the photoperiod, but more active under the photoperiod at 8:16h, lays eggs more some more; 3 kinds of photoperiods, the dead time occurs at the latest with 8:16h banyan pipe thrips adult, and general mortality rate is also minimum, after 7 days, only has 6.67%; At 10:14h or 6:18h, under the photoperiod, all have partial blade to occur that variable color and petiole come off, and 8:16h photoperiod lower blade is without variable color, at most just annesl a little appears in petiole.To sum up the display light cycle is best with the effect of 8:16h as a result.
table 5 photoperiod is to the activity of banyan pipe thrips adult, death and the fresh-keeping situation of blade
Comprehensive feeding condition exploratory experiment result, the batch of banyan pipe thrips is raised and should be selected colored mud as the moisturizing medium, and the water suction of flower mud is controlled at 8ml; The 2nd leaf or the 3rd leaf of feeding and newly taking out then banyan spray top, natural airing after blade will soak in the hydromel of 200 times of dilutions, and at the petiole place, the coated cotton mass that sucks 200 times of hydromels of dilution carries out fresh-keeping; To regulate and control the photoperiod is 8:16h simultaneously, and temperature is 26 ± 1 ℃, and humidity is 60% ± 5%.
(2) batch of banyan pipe thrips is raised
The batch of banyan pipe thrips is raised the collection that relates to ovum, several processes such as hatching, nymph raising and imago breeding of ovum.Material therefor has: special foster worm bottle, flower mud, filter paper, banyan branch and blade, absorbent cotton, hydromel, clear water, banyan pipe thrips adult etc.Whole operation refers to the procedure division of feeding of this patent, and every bottle is accessed 10 banyan pipe thrips adults with hooking the line writing brush, and male and female are than being 3:2, and raise result and show: the egg laying amount of female adult is at 50～70 intergranulars, and the incubation rate of ovum is more than 83.33%; Nymph was divided into for 4 ages, and the time that each instar larvae occurs and the length of time are all stable and more neat, and the nymph eclosion rate, up to more than 90.00%, can obtain the banyan pipe thrips adult of high vigor more than 200 in each foster worm bottle; The adult of raising can continue to keep high vigor, and growth is very neat, not there will be the death of adult.Illustrate that banyan pipe thrips can complete whole complete living from generation to generation with the method raising, realizes the vast scale amplification of insect amount.
two, example two: the real-time monitored of banyan pipe thrips
In the batch feeding process of banyan pipe thrips, by 3 times of magnifying glasses of bottle cap central authorities, Main Morphology feature and the growth course of each worm state of banyan pipe thrips to be observed, synthesis result is as follows:
(1) morphological feature in each length of time
ovum:ellipse or kidney shape, the overwhelming majority originates in the leaf goitre, occurs 1 pair of red eyespot before hatching.
1 age nymph:water white transparency while just incubating, the cephalothorax accounting is large, and merogenesis is not obvious, takes food the rear feeler spindle of decortication, takes action more active.
2 age nymph:the body yellow, the cephalothorax ratio diminishes, and merogenesis is obvious, there is no external wing bud, and action is active.
3 age nymph:the body yellow-white, feeler becomes very short cryptomere projection, grows large and obvious white wing bud, movable blunt, does not substantially take food, does not drain.
4 age nymph:body becomes dark color by milk yellow, feeler elongation and curved behind to head, and sheath shape wing bud is stretched and is reached belly 3/5 place, and activity is very blunt, just slowly movable while only sturting.
adult:the bodily form is elongated, pitchy; Head length is square, mouthpart file suction; Feeler 8 joints, the 3rd joint is the longest; The wing water white transparency, plumage tassel shape; Tail end is upturned, glossy.Female polypide is longer than male worm.
(2) the growth course in each length of time
The adult of feeding automatically proceeds in the branch insect gall in 3 days and lays eggs, and egg laying amount is larger.Ovum through about 7 days, hatch be 1 age nymph, incubation rate is higher; 1 age nymph transfer to voluntarily newly to take out then on the blade of spray top and take food and cause harm, about 3 days, develop into 2 age nymph; 2 age nymph take food cause harm about 8 days develop into 3 age nymph; 3 age nymph about 3 days, develop into 4 age nymph; 4 age nymph develop into adult about 5 days; The time that each instar larvae occurs and the length of time are all stable and more neat.Adult continues to keep high vigor, and growth is very neat, not there will be the death of adult; Adult often troops, and first along tender leaf master pulse both sides file, inhales juice, forms spot not of uniform size on leaf back, and then in edge, arteries and veins is folding to blade face, and greenery become Huang Ye gradually, forms pimple shape insect gall, and insect gall is covered with the bronzing spot outward; Proceed to adult 2-3d and carry out mating, more than mating time reaches 5min, male worm has the ability of lasting mating, and the female worm mating time in the male worm that firm mating is complete and other end is sustainable 3min left and right also; Adult post-coitum 12h left and right starts to lay eggs, and ovum is output in batches, irregular, lays eggs sustainable about 14 days.
Comprehensive above-mentioned observation, illustrate the demand that banyan pipe thrips is observed with the method raising energy full feeding, in metamorphosis, the history of life and the course that causes harm of indoor real-time monitored banyan pipe thrips.
special foster worm bottle:special foster worm bottle as described in Figure 1, is comprised of body 1 and bottle cap 2 two parts, and material is transparent organic glass; Body is taper type, footpath diameter 98mm suitable for reading, end opening footpath diameter 90mm, height 98mm, bottle sealing ground 5mm.The bottle cap circle, the annular protrusion of the thick 2.5mm of edge tool, high 15mm, press micro-supination and can combine closely with body; With magnifying glass 3, multiplication factor is 3 times, is positioned at bottle cap central authorities, diameter 80mm; With 8 ventilation holes 4, evenly ring-type is arranged in the magnifying glass periphery, and each ventilation hole diameter 5mm above is equipped with the above screen pack 5 of 80 orders that anti-banyan pipe thrips escapes.Special foster worm bottle has two large functions: the one, and can meet banyan pipe thrips and raise needed space and environment condition, ventilative and logical light; The 2nd, can meet the variation of real-time monitored banyan pipe thrips in whole growth and development process.
Claims (9)
1. one kind is utilized the method that the worm bottle is raised banyan pipe thrips of supporting, should support the worm bottle is comprised of body and bottle cap, it is characterized in that: the taper type that described body is big up and small down, described body and bottle cap sealing coordinate, the central authorities of described bottle cap are equipped with magnifying glass, be evenly distributed with a plurality of ventilation holes on described bottle cap, described ventilation hole is equipped with screen pack;
The method that worm bottle raising banyan pipe thrips is supported in described utilization comprises the following steps:
(1) collection of ovum: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather the banyan branch, 2～3 blades must be arranged on branch and at least will have an insect gall to exist; The branch blade is cleaned out, after in the branch bottom coated cotton mass, suck hydromel on cotton mass; Branch is vertically put into and supported the worm bottle, the state and do not contact a bottle wall of being kept upright; Banyan pipe thrips adult is received on the branch blade, and in the foster worm bottle shift-in phytotron that is connected to banyan pipe thrips, adult automatically proceeded in branch insect gall blade and lays eggs in 3 days, and remove adult postpartum, collected the insect gall blade of laying eggs, and obtained relatively consistent ovum;
(2) hatching of ovum: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather banyan and newly take out then the 2nd or the 3rd leaf on spray top, blade is cleaned out, be immersed in 25-35 second in hydromel, take out the nature airing, after at the petiole place coated cotton mass, suck hydromel on cotton mass; The blade level is put into and supported the worm bottle, do not contact bottle wall and blade face upward, the insect gall blade of laying eggs of collecting is put into subsequently and supported the worm bottle, then rest in worm bottle shift-in phytotron and hatch; Ovum hatching be 1 age nymph, and be transferred to voluntarily by the insect gall blade of laying eggs on the blade of collection and take food and cause harm;
(3) nymph is raised: the insect gall blade of laying eggs that step (2) is supported in the worm bottle is taken away, changes to the 2nd or the 3rd leaf that banyan is newly taken out the spray top then, the same step of processing method (2); Need to change fresh blade in the nymphal development process, make each nymph be transferred to voluntarily and newly change in blade by old blade in age, finally develop into adult;
(4) imago breeding: put into and colored mud of the same size at the bottom of bottle in the bottom of supporting the worm bottle, after water suction, on flower mud, put the onesize filter paper of a slice; Gather banyan and newly take out then shoot, have 3 blades on branch at least, the same step of branch processing method (1); Several branches that gather are progressively put into and supported the worm bottle, and built, keep substantially upright mutually, guarantees that the blade between each branch overlaps; To develop on the same day the blade of feeding of adult, relay gently on the built be comprised of branch, adult can be transferred on the branch blade voluntarily, after the blade of feeding from supporting in the worm bottle, take away, rest in worm bottle shift-in phytotron and raise;
The controlled condition of the phytotron that above each step adopts is: 25 ± 1 ℃ of temperature, humidity 60% ± 5%, photoperiod L:D=8:l6h.
2. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, and it is characterized in that: described body and bottle cap are made by transparent lucite.
3. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, it is characterized in that: the footpath suitable for reading diameter of described body is that 98mm, end opening footpath diameter are 90mm, highly are 98mm.
4. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 2, and it is characterized in that: the opening part of described body is provided with the sealing ground, and the height of sealing ground is 5mm; The edge of described bottle cap is provided with and the annular protrusion that seals ground and match, and the thickness of described annular protrusion is 2.5mm, highly is 15mm.
5. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, and it is characterized in that: the multiplication factor of described magnifying glass is 3 times, and diameter is 80mm.
6. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, and it is characterized in that: the quantity of described ventilation hole is 8, and the ventilation hole diameter is 5mm.
7. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, it is characterized in that: described screen pack is the above screen packs of 80 orders.
8. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, it is characterized in that: described hydromel is the hydromel of 200 times of dilutions.
9. the method that the worm bottle is raised banyan pipe thrips is supported in utilization according to claim 1, and it is characterized in that: the thickness of described colored mud is 5mm, water suction 6-8ml.
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| CN 201210018430 CN102524188B (en) | 2012-01-20 | 2012-01-20 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
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| CN103004698A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Method for measuring influence of humidity on scirtothrips dorsalis population |
| CN103004703A (en) * | 2012-12-21 | 2013-04-03 | 浙江农林大学 | Method for breeding gall-inducing harmful insects |
| CN103053477B (en) * | 2012-12-26 | 2014-01-22 | 中国农业科学院郑州果树研究所 | Scale breeding method of Scolothrips takahashii |
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| CN104094897B (en) * | 2014-02-17 | 2016-01-20 | 中国农业科学院植物保护研究所 | Beet webworm imago breeding utensil |
| CN104012472B (en) * | 2014-03-12 | 2016-08-17 | 云南农业大学 | The indoor feeding method of thrips |
| CN104782575B (en) * | 2014-12-09 | 2017-04-26 | 青岛农业大学 | Method for collecting and feeding tobacco thrips |
| CN104839098A (en) * | 2015-05-26 | 2015-08-19 | 中国农业科学院植物保护研究所 | Device and method for bioassay of piercing-sucking pests |
| CN104872068B (en) * | 2015-05-26 | 2018-11-16 | 中国热带农业科学院环境与植物保护研究所 | Worm device and method for breeding are supported in a kind of Huang chest thrips room |
| CN105409887B (en) * | 2015-10-26 | 2018-01-12 | 张志鹏 | A kind of method for breeding of palm thrips |
| CN107047485A (en) * | 2017-04-27 | 2017-08-18 | 福建省农业科学院茶叶研究所 | A kind of method of Cicadellidae insect indoor feeding and bionical raising box |
| CN107410217B (en) * | 2017-05-16 | 2023-06-06 | 中国热带农业科学院环境与植物保护研究所 | Breeding device and method for experimental population of cassava single-claw mites |
| CN110024921B (en) * | 2019-04-25 | 2022-10-14 | 福建省农业科学院植物保护研究所 | Application of biological stain xylene aniline in thrips insect membrane feeding |
| CN111466344B (en) * | 2020-04-23 | 2024-06-18 | 广西大学 | Method for continuously feeding thrips |
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2216742Y (en) * | 1995-01-25 | 1996-01-10 | 刘声树 | Bottle for breeding aphides |
| CN2850279Y (en) * | 2005-08-21 | 2006-12-27 | 刘声树 | Gall aphids culturing bottle |
| CN2865276Y (en) * | 2005-12-22 | 2007-02-07 | 云南农业大学 | Thripid insects indoor feeding pot |
| CN200987326Y (en) * | 2006-12-22 | 2007-12-12 | 池三丑 | Multifunction observing breeding bottle |
| CN101731183A (en) * | 2008-11-27 | 2010-06-16 | 甘肃农业大学 | Odontothrips loti feeding method |
| CN201509510U (en) * | 2009-08-27 | 2010-06-23 | 广东省农业科学院作物研究所 | Adult lepidopteron feeding device |
| CN202085609U (en) * | 2011-06-02 | 2011-12-28 | 长江大学 | Feeding device for small piercing-sucking mouthpart insects |
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