CN102523977A - Culture method for rice seedlings - Google Patents

Culture method for rice seedlings Download PDF

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CN102523977A
CN102523977A CN2011104080919A CN201110408091A CN102523977A CN 102523977 A CN102523977 A CN 102523977A CN 2011104080919 A CN2011104080919 A CN 2011104080919A CN 201110408091 A CN201110408091 A CN 201110408091A CN 102523977 A CN102523977 A CN 102523977A
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kimura
nutrient solution
rice seedling
culture
culturing
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CN102523977B (en
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张联合
王贺正
郁飞燕
李巍
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Henan University of Science and Technology
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Henan University of Science and Technology
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract

The invention discloses a culture method for rice seedlings. The rice seedlings are cultured by using a split root culture device; the side faces of two culture bowls of the device are butted; a cover plate and a cultivation plate are arranged above the culture bowls; the cultivation plate is provided with culture holes; the culture method comprises the following steps of: culturing 3-day rice seedlings in kimura nutrient solution with concentration of 50 percent for 2 days; replacing the kimura nutrient solution with the kimura nutrient solution with concentration of 100 percent for 12 days; taking consistently grown seedlings and wrapping stem base parts by using culture cotton; fixing in the culture holes of the cultivation plate; grouping rice seedling roots into equal groups A and B, wherein the group A is placed in the culture bowl containing the kimura nutrient solution, and the group B is placed in the culture bowl containing the culture solution of element deficient; adjusting the pH of the culture solution to 6.0 from 8 a.m. to 15 p.m. each day; and replacing the culture solution after culturing for 3 days, and after culturing to 4-12 days, taking the stems and leaves of the rice seedlings, the group A of cultured rice seedling roots and the group B of cultured rice seedling roots. The culture method is easy to operate; the personal error is avoided; the culture time is saved; and the working efficiency is increased.

Description

A kind of rice seedling cultural method
Technical field
The present invention relates to the cultural method of a kind of paddy rice, a kind of specifically split-root cultivation device that utilizes carries out cultured method to paddy rice.
Background technology
Paddy rice root division culture technique moves in the paddy rice body at physiological function, the nutritive element of research rice nutrition element, grow signal impression and the conduction and the root system gene expression etc. of influence, nutrient deficiency are widely used aspect many nutritive element to rice root.This technology relates to paddy rice split-root cultivation device and rice seedling cultural method.Utilizing split-root cultivation device to carry out the conventional method that rice seedling is cultivated, is rice seedling to be fixed on one cultivate on the plate, and it is to be isolated into two Nutrition Dept.s through central dividing plate that nutrient solution is cultivated alms bowl; This method and device, inconvenient fixation in rice seedling fixedly can influence the fixing of other seedling in the seedling simultaneously; When regulator solution pH value and replacing nutrient solution, occur a kind of nutrient solution easily and be splashed in the another kind of nutrient solution, thereby cause experimental result inaccurate; Be not easy to directly observe in the solution and have the many and big problem of error of disturbing factor in root growth situation and the incubation; Branch in the past is unreasonable with the culture apparatus structure, is awkward, and improves production cost; Reduce operating efficiency, increase labour expenditure.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, a kind of cultural method of rice seedling is provided, utilize split-root cultivation device that paddy rice is carried out cultured method, method is simple, and the paddy rice split-root cultivation device is rational in infrastructure, and easy operating is enhanced productivity.
The present invention addresses the above problem the technical scheme that is adopted to be: a kind of cultural method of rice seedling, and the employing split-root cultivation device is cultivated rice seedling, and split-root cultivation device comprises symmetrically arranged two culturing pots, cultivates plate and cover plate; Culturing pot is the cuboid of open upper end; The side butt joint of two culturing pots, the side of butt joint are provided with the cover plate and cultivation plate of polylith perpendicular to low side surface than the low 2.0cm of height of all the other sides above the openend of two culturing pots; Cover plate is alternately setting with the cultivation plate; Middle part cultivating plate is provided with culture hole, and the aperture of culture hole is 2.5-4.0cm, and culture hole is crossed on the interface top of two culturing pots; The length of described cover plate and cultivation plate all equates with two culturing pot width sums; Cover plate all is at least 4 with the cultivation plate;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and culture solution of element deficient respectively then; The injecting height of Kimura's nutrient solution and culture solution of element deficient is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 3.0-4.5cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into the culturing pot that contains culture solution of element deficient, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and culture solution of element deficient is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off cover plate and the cultivation plate that is fixed with rice seedling; Change Kimura's nutrient solution and culture solution of element deficient in two culturing pots respectively; Method according to step 3 and step 4; Rice seedling root A group is reentered in the culturing pot that contains Kimura's nutrient solution; Rice seedling root B group is reentered in the culturing pot that contains culture solution of element deficient, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling obtains the cauline leaf of rice seedling and the rice seedling root B that cultivates through the hydroponic rice seedling root of Huo Gelan A group and culture solution of element deficient organizes;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Described culture solution of element deficient is any one in nitrogen stress Kimura nutrient solution, scarce phosphorus Kimura nutrient solution, potassium deficiency Kimura's nutrient solution, a lack of sulfur Kimura nutrient solution, calcium deficiency Kimura's nutrient solution, magnesium deficiency Kimura nutrient solution, boron deficiency Kimura nutrient solution, scarce copper Kimura nutrient solution, scarce zinc Kimura nutrient solution, scarce molybdenum Kimura nutrient solution or the iron deficiency Kimura nutrient solution;
The composition of described nitrogen stress Kimura nutrient solution: the KH that contains 24.8mg in every liter of nitrogen stress Kimura nutrient solution 2PO 4, 31.32mg K 2SO 4, 25.84mg CaSO 4, 19.98mg CaCl 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce phosphorus Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce phosphorus Kimura nutrient solution 4) 2SO 4, the KCl of 13.59mg, the KNO of 18.5mg 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described potassium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of potassium deficiency Kimura nutrient solution 4) 2SO 4, 21.88mg NaH 2PO 4, 15.57mg NaNO 3, 12.98mg Na 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described a lack of sulfur Kimura nutrient solution: the NH that contains 14.8mg in every liter of a lack of sulfur Kimura nutrient solution 4NO 3, 24.8mg KH 2PO 4, 18.5mg KNO 3, the KCl of 13.41mg, the Ca (NO of 59.9mg 3) 2, 52.25mg MgCl 2, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described calcium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of calcium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 62.09mg NaNO 3, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described magnesium deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of magnesium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 77.98mg Na 2SO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described iron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of iron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 2.86mg H 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described boron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of boron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The CuSO of O, 0.08mg 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce copper Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce copper Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.22mg ZnSO 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce zinc Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce zinc Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce molybdenum Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce molybdenum Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2O, surplus is a water.
The invention has the beneficial effects as follows: the present invention utilizes split-root cultivation device that rice seedling is carried out cultured method; In the process of cultivating, independently culturing pot and polylith are cultivated plate and cover plates, more convenient rice seedling fixing to have adopted two; Can not have influence on the fixing of other seedling when fixing a strain seedling yet; The paddy rice split-root cultivation device of mentioning among the present invention is compared with culture apparatus in the past, only need open cover plate, can carry out the adjusting of pH value and observe rice seedling root growth situation; Save the operating time, reduce labour expenditure.
The split-root cultivation device of mentioning among the present invention, it is provided with rationally, and is simple to operate; Be convenient to wide popularization and application, cover plate and cultivation plate are opened, remove two culturing pots and change nutrient solution respectively; Avoid occurring a kind of nutrient solution and be splashed in the another kind of nutrient solution, thereby influence experimental result, cause the situation of human error; Improve culture efficiency, practice thrift cost.
The split-root cultivation device of mentioning among the present invention is convenient to control condition of culture, reduces labour expenditure, reduces the generation of disturbing factor, and operating efficiency improves more than 2.5 times, and test accuracy is improved, and result of study is more reliable.
Cultural method of the present invention is compared with conventional cultural method; Being divided into for two steps cultivates, and is that Kimura's nutrient solution of 50% was cultivated 2 days in concentration at first, and the back is that Kimura's nutrient solution of 100% is cultivated in concentration; Make rice seedling be easy to adapt to condition of culture; Avoid influencing cultivation results, save incubation time, increase work efficiency because of the unexpected transformation of condition of culture.
Description of drawings
Fig. 1 is the profile of split-root cultivation device;
Fig. 2 is the structural representation of split-root cultivation device;
Label is among the figure: 1, cover plate, 2, cultivate plate, 3, culture hole, 4, culturing pot.
Embodiment
A kind of cultural method of rice seedling, the employing split-root cultivation device is cultivated rice seedling, and split-root cultivation device comprises symmetrically arranged two culturing pots 4, cultivates plate 2 and cover plate 1; Culturing pot 4 is the cuboid of open upper end; The side butt joint of two culturing pots 4, the side of butt joint are provided with the cover plate 1 and cultivation plate 2 of polylith perpendicular to low side surface than the low 2.0cm of height of all the other sides above the openend of two culturing pots 4; Cover plate 1 is alternately setting with cultivation plate 2; Middle part cultivating plate 2 is provided with culture hole 3, and the aperture of culture hole 3 is 2.5-4.0cm, and culture hole 3 is crossed on the interface top of two culturing pots 4;
The length of described cover plate 1 equates with two culturing pots, 4 width sums;
Described cover plate 1 is at least 4;
The length of described cultivation plate 2 equates with two culturing pots, 4 width sums;
Described cultivation plate 2 is at least 4;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and culture solution of element deficient respectively then; The injecting height of Kimura's nutrient solution and culture solution of element deficient is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 3.0-4.5cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into the culturing pot that contains culture solution of element deficient, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and culture solution of element deficient is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off the cultivation plate and the cover plate that are fixed with rice seedling; Change Kimura's nutrient solution and culture solution of element deficient in two culturing pots respectively; Method according to step 3 and step 4; Rice seedling root A group is reentered in the culturing pot that contains Kimura's nutrient solution; Rice seedling root B group is reentered in the culturing pot that contains culture solution of element deficient, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling obtains the cauline leaf of rice seedling and the rice seedling root B that cultivates through the hydroponic rice seedling root of Huo Gelan A group and culture solution of element deficient organizes;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Described culture solution of element deficient is any one in nitrogen stress Kimura nutrient solution, scarce phosphorus Kimura nutrient solution, potassium deficiency Kimura's nutrient solution, a lack of sulfur Kimura nutrient solution, calcium deficiency Kimura's nutrient solution, magnesium deficiency Kimura nutrient solution, boron deficiency Kimura nutrient solution, scarce copper Kimura nutrient solution, scarce zinc Kimura nutrient solution, scarce molybdenum Kimura nutrient solution or the iron deficiency Kimura nutrient solution.
The composition of described nitrogen stress Kimura nutrient solution: the KH that contains 24.8mg in every liter of nitrogen stress Kimura nutrient solution 2PO 4, 31.32mg K 2SO 4, 25.84mg CaSO 4, 19.98mg CaCl 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce phosphorus Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce phosphorus Kimura nutrient solution 4) 2SO 4, the KCl of 13.59mg, the KNO of 18.5mg 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described potassium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of potassium deficiency Kimura nutrient solution 4) 2SO 4, 21.88mg NaH 2PO 4, 15.57mg NaNO 3, 12.98mg Na 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described a lack of sulfur Kimura nutrient solution: the NH that contains 14.8mg in every liter of a lack of sulfur Kimura nutrient solution 4NO 3, 24.8mg KH 2PO 4, 18.5mg KNO 3, the KCl of 13.41mg, the Ca (NO of 59.9mg 3) 2, 52.25mg MgCl 2, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described calcium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of calcium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 62.09mg NaNO 3, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described magnesium deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of magnesium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 77.98mg Na 2SO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described iron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of iron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 2.86mg H 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described boron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of boron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The CuSO of O, 0.08mg 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce copper Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce copper Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.22mg ZnSO 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce zinc Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce zinc Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
The composition of described scarce molybdenum Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce molybdenum Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2O, surplus is a water.
Embodiment one
A kind of cultural method of rice seedling, the employing split-root cultivation device is cultivated rice seedling, and split-root cultivation device comprises symmetrically arranged two culturing pots 4, cultivates plate 2 and cover plate 1; Culturing pot 4 is the cuboid of open upper end; The side butt joint of two culturing pots 4, the side of butt joint are provided with the cover plate 1 and cultivation plate 2 of polylith perpendicular to low side surface than the low 2.0cm of height of all the other sides above the openend of two culturing pots 4; Cover plate 1 is alternately setting with cultivation plate 2; Middle part cultivating plate 2 is provided with culture hole 3, and the aperture of culture hole 3 is 2.5cm, and culture hole 3 is crossed on the interface top of two culturing pots 4;
The length of described cover plate 1 equates with two culturing pots, 4 width sums;
Described cover plate 1 is at least 4;
The length of described cultivation plate 2 equates with two culturing pots, 4 width sums;
Described cultivation plate 2 is at least 4;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and scarce molybdenum Kimura nutrient solution then respectively; The injecting height of Kimura's nutrient solution and scarce molybdenum Kimura nutrient solution is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 3.0cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into and is contained the culturing pot that lacks molybdenum Kimura nutrient solution, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and scarce molybdenum Kimura nutrient solution is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off the cultivation plate and the cover plate that are fixed with rice seedling; Change Kimura's nutrient solution and scarce molybdenum Kimura nutrient solution in two culturing pots respectively,, rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution according to the method for step 3 and step 4; Rice seedling root B group is put into and is contained the culturing pot that lacks molybdenum Kimura nutrient solution, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling; The rice seedling root B group that cauline leaf of water intaking rice seedling and the hydroponic rice seedling root of process Huo Gelan A group and culture solution of element deficient are cultivated can be used as the experimental study of various objectives;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Described culture solution of element deficient is for lacking molybdenum Kimura nutrient solution.
The composition of described scarce molybdenum Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce molybdenum Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2O, surplus is a water.
Embodiment two
A kind of cultural method of rice seedling, the employing split-root cultivation device is cultivated rice seedling, and split-root cultivation device comprises symmetrically arranged two culturing pots 4, cultivates plate 2 and cover plate 1; Culturing pot 4 is the cuboid of open upper end; The side butt joint of two culturing pots 4, the side of butt joint are provided with the cover plate 1 and cultivation plate 2 of polylith perpendicular to low side surface than the low 2.0cm of height of all the other sides above the openend of two culturing pots 4; Cover plate 1 is alternately setting with cultivation plate 2; Middle part cultivating plate 2 is provided with culture hole 3, and the aperture of culture hole 3 is 3.5cm, and culture hole 3 is crossed on the interface top of two culturing pots 4;
The length of described cover plate 1 equates with two culturing pots, 4 width sums;
Described cover plate 1 is at least 4;
The length of described cultivation plate 2 equates with two culturing pots, 4 width sums;
Described cultivation plate 2 is at least 4;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and scarce zinc Kimura nutrient solution then respectively; The injecting height of Kimura's nutrient solution and scarce zinc Kimura nutrient solution is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 4.0cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into and is contained the culturing pot that lacks zinc Kimura nutrient solution, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and scarce zinc Kimura nutrient solution is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off the cultivation plate and the cover plate that are fixed with rice seedling; Change Kimura's nutrient solution and scarce zinc Kimura nutrient solution in two culturing pots respectively,, rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution according to the method for step 3 and step 4; Rice seedling root B group is put into and is contained the culturing pot that lacks zinc Kimura nutrient solution, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling; The rice seedling root B group that cauline leaf of water intaking rice seedling and the hydroponic rice seedling root of process Huo Gelan A group and culture solution of element deficient are cultivated can be used as the experimental study of various objectives;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 200mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Described culture solution of element deficient is for lacking zinc Kimura nutrient solution.
The composition of described scarce zinc Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce zinc Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Embodiment three
A kind of cultural method of rice seedling, the employing split-root cultivation device is cultivated rice seedling, and split-root cultivation device comprises symmetrically arranged two culturing pots 4, cultivates plate 2 and cover plate 1; Culturing pot 4 is the cuboid of open upper end; The side butt joint of two culturing pots 4, the side of butt joint are provided with the cover plate 1 and cultivation plate 2 of polylith perpendicular to low side surface than the low 2.0cm of height of all the other sides above the openend of two culturing pots 4; Cover plate 1 is alternately setting with cultivation plate 2; Middle part cultivating plate 2 is provided with culture hole 3, and the aperture of culture hole 3 is 4.0cm, and culture hole 3 is crossed on the interface top of two culturing pots 4;
The length of described cover plate 1 equates with two culturing pots, 4 width sums;
Described cover plate 1 is at least 4;
The length of described cultivation plate 2 equates with two culturing pots, 4 width sums;
Described cultivation plate 2 is at least 4;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and magnesium deficiency Kimura nutrient solution then respectively; The injecting height of Kimura's nutrient solution and magnesium deficiency Kimura nutrient solution is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 4.5cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into the culturing pot that contains magnesium deficiency Kimura nutrient solution, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and magnesium deficiency Kimura nutrient solution is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off the cultivation plate and the cover plate that are fixed with rice seedling; Change Kimura's nutrient solution and magnesium deficiency Kimura nutrient solution in two culturing pots respectively,, rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution according to the method for step 3 and step 4; Rice seedling root B group is put into the culturing pot that contains magnesium deficiency Kimura nutrient solution, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling; The rice seedling root B group that cauline leaf of water intaking rice seedling and the hydroponic rice seedling root of process Huo Gelan A group and culture solution of element deficient are cultivated can be used as the experimental study of various objectives;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.
Described culture solution of element deficient is a magnesium deficiency Kimura nutrient solution.
The composition of described magnesium deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of magnesium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 77.98mg Na 2SO 4, 200mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water.

Claims (1)

1. rice seedling cultural method; It is characterized in that: adopt split-root cultivation device cultivating rice seedling; Split-root cultivation device comprises symmetrically arranged two culturing pots (4), cultivates plate (2) and cover plate (1), and culturing pot (4) is the cuboid of open upper end, the side butt joint of two culturing pots (4); The side of butt joint is than the low 2.0cm of height of all the other sides; Above the openend of two culturing pots (4), be provided with cover plate (1) and the cultivation plate (2) of polylith perpendicular to low side surface, cover plate (1) is alternately setting with cultivation plate (2), is provided with culture hole (3) at the middle part of cultivation plate (2); The aperture of culture hole (3) is 2.5-4.0cm, and culture hole (3) is crossed on the interface top of two culturing pots (4); The length of described cover plate (1) and cultivation plate (2) all equates with two culturing pots (4) width sum; Cover plate (1) and cultivation plate (2) all are at least 4;
The method that adopts split-root cultivation device to cultivate rice seedling is:
Step 1, get the rice seedling of 3 ages in days, in concentration is 50% Huo Gelan nutrient solution, cultivate 2 days after, be replaced by concentration and be 100% Huo Gelan nutrient solution and continue to cultivate 12 days, choose the consistent rice seedling of growth then, subsequent use;
Step 2, with two symmetrically arranged culturing pots butt joints; In symmetrically arranged two culturing pots, add Kimura's nutrient solution and culture solution of element deficient respectively then; The injecting height of Kimura's nutrient solution and culture solution of element deficient is than the low 1.0cm of height of two culturing pot interfaces, and is subsequent use;
Step 3, water intaking rice seedling on the basal part of stem of rice seedling, are wrapped up in the cultivation bale of cotton apart from rice seedling root 1.0cm place; Cultivating cotton parcel thickness is 3.0-4.5cm; Cultivate cotton rice seedling basal part of stem and be fixed in the culture hole of cultivating on the plate being enclosed with, rice seedling is positioned at the interface top of two culturing pots, by the root bar number of rice seedling the rice seedling root is divided into equal two groups; Be rice seedling root A group and rice seedling root B group; Rice seedling root A group is put into the culturing pot that contains Kimura's nutrient solution, and rice seedling root B group is put into the culturing pot that contains culture solution of element deficient, cultivates plate and is installed in the culturing pot top; In a side of cultivating plate cover plate is installed, subsequent use;
Step 4, according to the method for step 3, successively by strain fixation in rice seedling until openend sealing with two culturing pots, rice seedling is cultivated;
Step 5,8 of every mornings and afternoons 15 point, open at least 1 cover plate, the pH value of Kimura's nutrient solution and culture solution of element deficient is transferred to 6.0, cover cover plate then, continue to cultivate;
Step 6, every cultivation are after 3 days; Take off cover plate and the cultivation plate that is fixed with rice seedling; Change Kimura's nutrient solution and culture solution of element deficient in two culturing pots respectively; Method according to step 3 and step 4; Rice seedling root A group is reentered in the culturing pot that contains Kimura's nutrient solution; Rice seedling root B group is reentered in the culturing pot that contains culture solution of element deficient, continues to cultivate;
Step 7, set by step five and after the method for step 6 is cultured to 4-12 days with rice seedling obtains the cauline leaf of rice seedling and the rice seedling root B that cultivates through the hydroponic rice seedling root of Huo Gelan A group and culture solution of element deficient organizes;
The composition of described Kimura's nutrient solution: (NH that contains 48.2mg in every litre village nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
Described culture solution of element deficient is any one in nitrogen stress Kimura nutrient solution, scarce phosphorus Kimura nutrient solution, potassium deficiency Kimura's nutrient solution, a lack of sulfur Kimura nutrient solution, calcium deficiency Kimura's nutrient solution, magnesium deficiency Kimura nutrient solution, boron deficiency Kimura nutrient solution, scarce copper Kimura nutrient solution, scarce zinc Kimura nutrient solution, scarce molybdenum Kimura nutrient solution or the iron deficiency Kimura nutrient solution;
The composition of described nitrogen stress Kimura nutrient solution: the KH that contains 24.8mg in every liter of nitrogen stress Kimura nutrient solution 2PO 4, 31.32mg K 2SO 4, 25.84mg CaSO 4, 19.98mg CaCl 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described scarce phosphorus Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce phosphorus Kimura nutrient solution 4) 2SO 4, the KCl of 13.59mg, the KNO of 18.5mg 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described potassium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of potassium deficiency Kimura nutrient solution 4) 2SO 4, 21.88mg NaH 2PO 4, 15.57mg NaNO 3, 12.98mg Na 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described a lack of sulfur Kimura nutrient solution: the NH that contains 14.8mg in every liter of a lack of sulfur Kimura nutrient solution 4NO 3, 24.8mg KH 2PO 4, 18.5mg KNO 3, the KCl of 13.41mg, the Ca (NO of 59.9mg 3) 2, 52.25mg MgCl 2, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described calcium deficiency Kimura's nutrient solution: (NH that contains 48.2mg in every liter of calcium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 62.09mg NaNO 3, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described magnesium deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of magnesium deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 77.98mg Na 2SO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described iron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of iron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 2.86mg H 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described boron deficiency Kimura the nutrient solution: (NH that contains 48.2mg in every liter of boron deficiency Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The CuSO of O, 0.08mg 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described scarce copper Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce copper Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.22mg ZnSO 47H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described scarce zinc Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce zinc Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The MnCl of O, 1.81mg 24H 2The H of O, 0.09mg 2Mo4H 2O, surplus is a water;
The composition of described scarce molybdenum Kimura the nutrient solution: (NH that contains 48.2mg in every liter of scarce molybdenum Kimura nutrient solution 4) 2SO 4, 24.8mg KH 2PO 4, 18.5mg KNO 3, 15.9mg K 2SO 4, 59.9mg Ca (NO 3) 2, 65.9mg MgSO 4, 100-300mg Na 2SiO 4, 8.42mg C 10H 12N 2O 8FeNa3H 2The H of O, 2.86mg 2BO 3, 0.08mg CuSO 45H 2The ZnSO of O, 0.22mg 47H 2The MnCl of O, 1.81mg 24H 2O, surplus is a water.
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