CN102516319A - Method for extracting phenolic compounds from sugarcane tips - Google Patents
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Abstract
The invention discloses a method for extracting phenolic compounds from sugarcane tips. The method comprises the following steps of: removing impurities from sugarcane tips, smashing, leaching the smashed sugarcane tips with ethanol, combining an extracting solution with sugarcane tip juice, filtering, heating, concentrating, combining juice to obtain crude extracted polyphenol serving as a concentrate, recovering an evaporated organic solvent, and measuring the polyphenol content with a Folin's method; and adjusting the pH of the crude extracted polyphenol, filtering with a filter film, loading a sample onto a polyamide chromatography column, eluting with an ethanol solution, collecting an eluent, and concentrating to obtain pure polyphenol. Due to the adoption of the method disclosed by the invention, the raw material cost is reduced, experimental operation is simplified, the working efficiency is increased, the extracting rate and purity of a target product are increased, and a method for measuring total phenolics in sugarcane tips and specific processes for extracting, separating and purifying polyphenol are established simultaneously.
Description
Technical field
The invention belongs to the method for from sugarcane toppers, extracting aldehydes matter.
Background technology
Along with plant polyphenol chemistry and pharmacological development, people recognize that gradually polyphenol is one type of natural product with unique physiologically active and pharmacologically active.Along with the rise of current " pursuit nature " consumption idea, polyphenol has broad application prospects in high-tech areas such as pharmacy, biochemistry, daily use chemicals, food and fine chemistry industries.
The sugarcane tip is sugarcane stem vegetative point (sugarcane is yellow) position, place, and when sugarcane growth course (except that after blooming), the nutriment of sugarcane stem offers vegetative point as far as possible, so contain multiple inorganic elements, protein, starch, VITAMINs, amino acid, phenols, enzyme etc.Polyphenol content in the sugarcane is very high, especially sugarcane taper branch.
At present had the polyphenol of various plants to be developed by people, raw materials cost is higher but the high plant of some polyphenol content is used for doing extraction.Millions of tons the sugarcane tip is arranged in Guangxi every year, except that few part is made feed or reserved seed for planting, most ofly burn as faggot, little to the degree of its processing and utilization, do not bring into play resources advantage.The sugarcane tip is a kind of cheap raw material that extracts polyphenol; Can it be squeezed the juice according to the composition of the sugarcane sugarcane tip and extract the Polyphenols thing; All right fermentation alcohol of by product after carrying and making bagasse feed etc. fully develop its potential value, benefit to driving local economy.
Summary of the invention
The technical problem that the present invention will solve provides a kind of method that can turn waste into wealth, make full use of resource, make raw material extraction aldehydes matter of sugarcane toppers.
The present invention solves the problems of the technologies described above with following technical scheme:
A kind of method of from sugarcane toppers, extracting aldehydes matter, its process step is:
Step 1 is removed sugarcane toppers impurity such as outside Lao Ye and earth and through the blanching enzyme that goes out, is cut into segment, puts into the crusher fragmentation; Get broken good sugarcane toppers and use the ethanol lixiviate, vat liquor and sugarcane tip pressure water are merged, filter; Heating concentrates and merges juice; The gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol, measures polyphenol content with folin's methods;
Step 2 is got polymeric amide, with alcohol immersion, constantly stirs, and removes in the void column of packing into behind the bubble; Polymeric amide is packed into behind the void column, successively uses ethanol, the NaOH aqueous solution, zero(ppm) water, acetic acid aqueous solution wash-out, is eluted to pH neutrality with zero(ppm) water at last, obtains polymeric amide chromatography post;
It is 2.06～17.14mgmL that step 3 is got initial mass concentration
-1Slightly carry polyphenol solution, using concentrated hydrochloric acid to regulate pH is 4, with 0.45 μ m membrane filtration, goes up a kind polymeric amide chromatography post that arrives step 2 gained afterwards, placement is spent the night, and makes abundant absorption; Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, carry out wash-out with ethanolic soln again, collect elutriant, concentrate, obtain pure article polyphenol.1
Sugarcane toppers in the step 1 will pass through the blanching enzyme that goes out and handle, and the temperature of blanching is 80 ± 2 ℃, and clear water is as scald liquor, and blanching treatment time is 2min; The sugarcane toppers that blanching is good immerses in the mixture of ice and water, and the sugarcane toppers temperature is reduced to below 10 ℃; Carry out draining immediately through cooled sugarcane toppers, cause error in order to avoid residuary water gets into the extraction link.2
The described sugarcane toppers of step 1 is cut into the segment of 0.4～0.7cm, 20～70 ℃ of extraction temperatures.3
The temperature of the described lixiviate of step 1 is 55～65 ℃, and the mass percentage concentration of digestion agent ethanolic soln is 55～65%, and solid-liquid ratio is 1: 2～4, and leaching time is 1～3h.4
The measuring method of the described folin's methods mensuration of step 1 polyphenol content is represented with the equivalent value TAE of Weibull; The FD reagent volume is 1～4mL, FD reagent and saturated Na
2CO
3The volume ratio of solution is 2: 1～6,20～30 ℃ of room temperatures, and the reaction times is 0.5～1.5h.5
During step 2 preparation polymeric amide chromatography post, the aperture of polymeric amide is 150～180 μ m, and the pH value is 2～6, and last appearance flow velocity is 2～6mLmin
-1, it is 1.5～2.5h that required time is stablized in the entering of experiment situation; On the polymeric amide behind the void column, the mass percentage concentration of using 3～4 times of volumes is 90%～95% ethanol elution, be washed till elutriant transparent and behind evaporate to dryness no residue or few residue; The mass percentage concentration of using 2～2.5 times of volumes more successively is 10% acetic acid aqueous solution wash-out as the mass percentage concentration of the zero(ppm) water of the 5%NaOH aqueous solution, 1 times of volume, 2～2.5 times of volumes, is eluted to pH neutrality with zero(ppm) water at last.6
After polymeric amide chromatography post in the step 3 fully adsorbed, using mass percentage concentration was that 20%～80% ethanolic soln carries out wash-out.7
Total phenol content is measured formula:
In the formula: X
1-according to typical curve calculate the sample concentration value, mgL
-1
V
1-diluted sample multiple;
V
2-sample liquids TV, L;
The W-raw material is heavy, g.8
The selection Weibull is a standard reference material, and with Weibull drawing standard curvilinear equation, optimum absorb wavelength is 725nm.9
During with Weibull drawing standard curvilinear equation, accurately drawing concentration is 0.2mgmL
-1Weibull standardized solution 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,4.5,5.0mL in the 100mL volumetric flask, be made into 1,2,3,4,5,6,7,8,9,10mgL
-1The standardized solution of different concns; Add FD reagent 3mL, fully add saturated sodium carbonate solution 4.5mL behind the mixing, after 1 hour; Be settled to scale with zero(ppm) water, measure absorbance, replace sample to do blank with zero(ppm) water; Obtaining Weibull typical curve equation is: Y=0.0436x+0.167, Y are absorbancy, and x is a polyphenol content.10
Method of the present invention has not only reduced raw materials cost, has simplified experimental implementation, has improved working efficiency, improved the extraction yield and the purity of title product, has set up the method for determining total phenol in the sugarcane toppers simultaneously and polyphenol extracts and the concrete technology of separation and purification.
Embodiment
The invention provides means that a kind of physics and chemical process combine and from sugarcane toppers, extract the method for aldehydes matter.In the method for the invention, the condition definite and the polymeric amide adsorbing polyphenols of the selection of digestion agent and environmental factors is to realize key of the present invention in the leaching process.Present method comprises that getting broken good sugarcane toppers to use mass percentage concentration be 55%～65% ethanol in temperature is lixiviate under the condition that 55 ℃～65 ℃, solid-liquid ratio are 1: 2～4, leaching time is 1～3h; Vat liquor and sugarcane tip pressure water are merged; Filter; Heating concentrates and merges juice, and the gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol.Getting polymeric amide is 90%～95% alcohol immersion with mass percentage concentration, constantly stirs, and removes in the void column of packing into behind the bubble.With mass percentage concentration 90%～95% ethanol elution of 4 times of volumes, it is transparent and behind evaporate to dryness, do not have a residue (or few residue) to be washed till elutriant.Use the mass percentage concentration 10% acetic acid aqueous solution wash-out of the zero(ppm) water of the mass percentage concentration 5%NaOH aqueous solution of 2.5 times of volumes, 1 times of volume, 2.5 times of volumes more successively, be eluted to pH neutrality with zero(ppm) water at last, obtain polymeric amide chromatography post.Getting initial mass concentration is 2.06～17.14mgmL
-1Slightly carry polyphenol solution, with 0.45 μ m membrane filtration, regulating pH value is 4, is 2～6mLmin at last kind of flow velocity
-1Condition under, last appearance is to polymeric amide chromatography post, placement is spent the night, and makes abundant absorption.Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, using mass percentage concentration again is that 20%～80% ethanolic soln carries out wash-out, collects elutriant, concentrates, and obtains pure article polyphenol.
Embodiment 1
Get sugarcane toppers and carry out the blanching enzyme that goes out and handle, the temperature of blanching is 80 ℃, and clear water is as scald liquor, and blanching treatment time is 2min.In the sugarcane toppers submerged mixture of ice and water that blanching is good, required ice needs in refrigerator, to make earlier, and the sugarcane toppers temperature is reduced to below 10 ℃.Carry out draining immediately through cooled sugarcane toppers, cause error in order to avoid residuary water gets into the extraction link.Pretreated sugarcane toppers is cut into the segment of about 0.4cm, and it is broken to put into crusher, and getting ethanol that broken good sugarcane toppers uses mass percentage concentration 55% is that 55 ℃, solid-liquid ratio are 1: 2 in temperature; Leaching time is lixiviate under the condition of 1h; Vat liquor and sugarcane tip pressure water are merged, filter, heating concentrates and merges juice; The gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol.Get polymeric amide 4g with mass percentage concentration 95% alcohol immersion, constantly stir, remove in the void column of packing into behind the bubble.Using the mass percentage concentration of 4 times of volumes is 95% ethanol elution, and it is transparent and behind evaporate to dryness, do not have a residue to be washed till elutriant.Use the mass percentage concentration 10% acetic acid aqueous solution wash-out of the zero(ppm) water of the mass percentage concentration 5%NaOH aqueous solution of 2.5 times of volumes, 1 times of volume, 2.5 times of volumes more successively, be eluted to pH neutrality with zero(ppm) water at last, obtain polymeric amide chromatography post.Getting initial mass concentration is 2.06mgmL
-1Slightly carry polyphenol solution, with 0.45 μ m membrane filtration, regulating pH value is 4, is 4mLmin at last kind of flow velocity
-1Condition under, last appearance is to polyamide column, placement is spent the night, and makes abundant absorption.Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, using mass percentage concentration again is that 40% ethanolic soln carries out wash-out, collects elutriant, concentrates, and obtains pure article polyphenol.
Embodiment 2
Get sugarcane toppers and carry out the blanching enzyme that goes out and handle, the temperature of blanching is 81 ℃, and clear water is as scald liquor, and blanching treatment time is 2min.In the sugarcane toppers submerged mixture of ice and water that blanching is good, required ice needs in refrigerator, to make earlier, and the sugarcane toppers temperature is reduced to below 10 ℃.Carry out draining immediately through cooled sugarcane toppers, cause error in order to avoid residuary water gets into the extraction link.Pretreated sugarcane toppers is chopped to the segment of about 0.6cm with cutter; It is broken to put into crusher, get broken good sugarcane toppers uses mass percentage concentration 60% ethanol temperature be 60 ℃, solid-liquid ratio be 1: 3, leaching time as the condition of 2h under lixiviate, with vat liquor and the merging of sugarcane tip pressure water; Filter; Heating concentrates and merges juice, and the gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol.Get polymeric amide 4g with mass percentage concentration 95% alcohol immersion, constantly stir, remove in the void column of packing into behind the bubble.With mass percentage concentration 95% ethanol elution of 4 times of volumes, be washed till elutriant transparent and behind evaporate to dryness few residue.Use the mass percentage concentration 10% acetic acid aqueous solution wash-out of the zero(ppm) water of the mass percentage concentration 5%NaOH aqueous solution of 2.5 times of volumes, 1 times of volume, 2.5 times of volumes more successively, be eluted to pH neutrality with zero(ppm) water at last, obtain polymeric amide chromatography post.Getting initial mass concentration is 9.05mgmL
-1The sugarcane toppers extracting solution, with 0.45 μ m membrane filtration, regulating pH value is 4, is 2mLmin at last kind of flow velocity
-1Condition under, last appearance is to polyamide column, placement is spent the night, and makes abundant absorption.Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, using concentration again is that 50% ethanolic soln carries out wash-out, collects elutriant, concentrates, and obtains pure article polyphenol.
Embodiment 3
Get sugarcane toppers and carry out the blanching enzyme that goes out and handle, the temperature of blanching is 79 ℃, and clear water is as scald liquor, and blanching treatment time is 2min.In the sugarcane toppers submerged mixture of ice and water that blanching is good, required ice needs in refrigerator, to make earlier, and the sugarcane toppers temperature is reduced to below 10 ℃.Carry out draining immediately through cooled sugarcane toppers, cause error in order to avoid residuary water gets into the extraction link.Pretreated sugarcane toppers is chopped to the segment of about 0.7cm with cutter; It is broken to put into crusher, get broken good sugarcane toppers uses mass percentage concentration 65% ethanol temperature be 65 ℃, solid-liquid ratio be 1: 4, leaching time as the condition of 3h under lixiviate, with vat liquor and the merging of sugarcane tip pressure water; Filter; Heating concentrates and merges juice, and the gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol.Get polymeric amide with mass percentage concentration 90% alcohol immersion, constantly stir, remove in the void column of packing into behind the bubble.With mass percentage concentration 90% ethanol elution of 4 times of volumes, it is transparent and behind evaporate to dryness, do not have a residue to be washed till elutriant.Use the mass percentage concentration 10% acetic acid aqueous solution wash-out of the zero(ppm) water of the mass percentage concentration 5%NaOH aqueous solution of 2.5 times of volumes, 1 times of volume, 2.5 times of volumes more successively, be eluted to pH neutrality with zero(ppm) water at last, obtain polymeric amide chromatography post.Getting initial mass concentration is 17.14mgmL
-1The sugarcane toppers extracting solution, with 0.45 μ m membrane filtration, regulating pH value is 4, is 6mLmin at last kind of flow velocity
-1Condition under, last appearance is to polyamide column, placement is spent the night, and makes abundant absorption.Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, using mass percentage concentration again is that 60% ethanolic soln carries out wash-out, collects elutriant, concentrates, and obtains pure article polyphenol.
Claims (10)
1. method of from sugarcane toppers, extracting aldehydes matter is characterized in that process step is:
Step 1 is removed sugarcane toppers impurity such as outside Lao Ye and earth and through the blanching enzyme that goes out, is cut into segment, puts into the crusher fragmentation; Get broken good sugarcane toppers and use the ethanol lixiviate, vat liquor and sugarcane tip pressure water are merged, filter; Heating concentrates and merges juice; The gained enriched material reclaims the evaporable organic solvent for slightly carrying polyphenol, measures polyphenol content with folin's methods;
Step 2 is got polymeric amide, with alcohol immersion, constantly stirs, and removes in the void column of packing into behind the bubble; Polymeric amide is packed into behind the void column, successively uses ethanol, the NaOH aqueous solution, zero(ppm) water, acetic acid aqueous solution wash-out, is eluted to pH neutrality with zero(ppm) water at last, obtains polymeric amide chromatography post;
It is 2.06～17.14mgmL that step 3 is got initial mass concentration
-1Slightly carry polyphenol solution, using concentrated hydrochloric acid to regulate pH is 4, with 0.45 μ m membrane filtration, goes up a kind polymeric amide chromatography post that arrives step 2 gained afterwards, placement is spent the night, and makes abundant absorption; Earlier carry out wash-out, remove the big impurity component of depolarization with purified water, be washed till the Molish reaction negative after, carry out wash-out with ethanolic soln again, collect elutriant, concentrate, obtain pure article polyphenol.
2. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 1; It is characterized in that sugarcane toppers in the step 1 will pass through the blanching enzyme that goes out and handle; The temperature of blanching is 80 ± 2 ℃; Clear water is as scald liquor, and blanching treatment time is 2min: the sugarcane toppers that blanching is good immerses in the mixture of ice and water, and the sugarcane toppers temperature is reduced to below 10 ℃; Carry out draining immediately through cooled sugarcane toppers, cause error in order to avoid residuary water gets into the extraction link.
3. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 1 is characterized in that 20～70 ℃ of sugarcane toppers extraction temperatures in the step 1.
4. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 3; The temperature that it is characterized in that the described lixiviate of step 1 is 55～65 ℃; The mass percentage concentration of digestion agent ethanolic soln is 55～65%, and solid-liquid ratio is 1: 2～4, and leaching time is 1～3h.
5. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 1 is characterized in that measuring method that the described folin's methods of step 1 measures polyphenol content representes with the equivalent value TAE of Weibull; The FD reagent volume is 1～4mL, and the volume ratio of FD reagent and saturated Na2CO3 solution is 2: 1～6,20～30 ℃ of room temperatures, and the reaction times is 0.5～1.5h.
6. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 1, when it is characterized in that step 2 preparation polymeric amide chromatography post, the aperture of polymeric amide is 150～180 μ m, and the pH value is 2～6, and last appearance flow velocity is 2～6mLmin
-1, it is 1.5～2.5h that required time is stablized in the entering of experiment situation; On the polymeric amide behind the void column, using 3～4 times of volume mass percentage concentrations is 90%～95% ethanol elution, be washed till elutriant transparent and behind evaporate to dryness no residue or few residue; The mass concentration of using 2～2.5 times of volumes more successively is 10% acetic acid aqueous solution wash-out as the mass percentage concentration of the zero(ppm) water of the 5%NaOH aqueous solution, 1 times of volume, 2～2.5 times of volumes, is eluted to pH neutrality with zero(ppm) water at last.
7. from sugarcane toppers, extract the method for aldehydes matter according to claim 1, it is characterized in that polymeric amide chromatography post in the step 3 fully adsorbs after, using mass percentage concentration is that 20%～80% ethanolic soln carries out wash-out.
8. from sugarcane toppers, extract the method for aldehydes matter according to claim 1, it is characterized in that total phenol content mensuration formula is:
In the formula: X
1-according to typical curve calculate the sample concentration value, mgL
-1
V
1-diluted sample multiple;
V
2-sample liquids TV, L;
The W-raw material is heavy, g.
9. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 8, it is characterized in that selecting Weibull is standard reference material, with Weibull drawing standard curvilinear equation, optimum absorb wavelength is 725nm.
10. the method for from sugarcane toppers, extracting aldehydes matter as claimed in claim 9, when it is characterized in that with Weibull drawing standard curvilinear equation, accurately drawing concentration is 0.2mgmL
-1Weibull standardized solution 0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,4.5,5.0mL in the 100mL volumetric flask, be made into 1,2,3,4,5,6,7,8,9,10mgL
-1The standardized solution of different concns; Add FD reagent 3mL, fully add saturated sodium carbonate solution 4.5mL behind the mixing, after 1 hour; Be settled to scale with zero(ppm) water, measure absorbance, replace sample to do blank with zero(ppm) water; Obtaining Weibull typical curve equation is: Y=0.0436x+0.167, Y are absorbancy, and x is a polyphenol content.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104957602A (en) * | 2015-05-22 | 2015-10-07 | 长沙普源生物科技有限公司 | Sugarcane bioactive component composition, functional health food, and preparation method of sugarcane bioactive component composition and functional health food |
| CN105348331A (en) * | 2015-11-05 | 2016-02-24 | 广西大学 | Extraction method of plant polyphenol |
| CN105816702A (en) * | 2016-03-31 | 2016-08-03 | 华南理工大学 | Sugarcane polyphenols extract and preparation method and application thereof |
| CN106075108A (en) * | 2016-06-14 | 2016-11-09 | 安徽平唐微食疗科技有限公司 | A kind of extracting method of Caulis Sacchari sinensis polyphenol |
| CN106177555A (en) * | 2016-08-18 | 2016-12-07 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method extracting Caulis Sacchari sinensis polyphenol from Caulis Sacchari sinensis scum silica frost |
| CN106220690A (en) * | 2016-07-25 | 2016-12-14 | 广东省生物工程研究所（广州甘蔗糖业研究所） | The extracting method of polyphenol in a kind of Caulis Sacchari sinensis scum silica frost |
| CN107522754A (en) * | 2017-08-31 | 2017-12-29 | 浙江大学 | A kind of method for extracting sugarcane skin polyphenol |
| CN107640964A (en) * | 2017-11-09 | 2018-01-30 | 江西新景象陶瓷有限公司 | A kind of ceramic material for filter water and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003506010A (en) * | 1999-03-29 | 2003-02-18 | アンスティテュ ナスィヨナル ドゥ ラ ルシェルシュ アグロナミーク | Method for obtaining juice based on sugarcane, juice thus obtained, and beverage containing said juice |
| CN101553582A (en) * | 2006-09-19 | 2009-10-07 | 视界科技有限公司 | Extracts derived from sugar cane and a process for their manufacture |
| CN101854811A (en) * | 2007-10-04 | 2010-10-06 | 南方化学股份公司 | The removal of the unwanted impurity of phytoprotein extract |
-
2011
- 2011-12-08 CN CN2011104061706A patent/CN102516319A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003506010A (en) * | 1999-03-29 | 2003-02-18 | アンスティテュ ナスィヨナル ドゥ ラ ルシェルシュ アグロナミーク | Method for obtaining juice based on sugarcane, juice thus obtained, and beverage containing said juice |
| CN101553582A (en) * | 2006-09-19 | 2009-10-07 | 视界科技有限公司 | Extracts derived from sugar cane and a process for their manufacture |
| CN101854811A (en) * | 2007-10-04 | 2010-10-06 | 南方化学股份公司 | The removal of the unwanted impurity of phytoprotein extract |
Non-Patent Citations (3)
| Title |
|---|
| BERTRAND PAYET,等: "Assessment of Antioxidant Activity of Cane Brown Sugars by ABTS and DPPH Radical Scavenging Assays: Determination of Their Polyphenolic and Volatile Constituents", 《J. AGRIC. FOOD CHEM.》, vol. 53, no. 26, 3 December 2005 (2005-12-03), pages 10074 - 10079 * |
| 孙晓雪: "甘蔗梢中酚类物质的提取与应用研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》, no. 5, 1 June 2007 (2007-06-01) * |
| 林金梅，等: "Folin-Ciocalteu法测定甘蔗糖厂制品的总酚含量", 《食品科技》, vol. 35, no. 6, 20 June 2010 (2010-06-20), pages 278 - 280 * |
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| CN104957602A (en) * | 2015-05-22 | 2015-10-07 | 长沙普源生物科技有限公司 | Sugarcane bioactive component composition, functional health food, and preparation method of sugarcane bioactive component composition and functional health food |
| CN105348331A (en) * | 2015-11-05 | 2016-02-24 | 广西大学 | Extraction method of plant polyphenol |
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| CN106177555B (en) * | 2016-08-18 | 2019-07-12 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A method of extracting sugarcane polyphenol from sugarcane dross |
| CN106177555A (en) * | 2016-08-18 | 2016-12-07 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method extracting Caulis Sacchari sinensis polyphenol from Caulis Sacchari sinensis scum silica frost |
| CN107522754A (en) * | 2017-08-31 | 2017-12-29 | 浙江大学 | A kind of method for extracting sugarcane skin polyphenol |
| CN107640964A (en) * | 2017-11-09 | 2018-01-30 | 江西新景象陶瓷有限公司 | A kind of ceramic material for filter water and preparation method thereof |
| CN110771778A (en) * | 2019-11-26 | 2020-02-11 | 福州大学 | Method for reducing heterocyclic amine content in dried pork slices by utilizing cane molasses extract |
| CN110771778B (en) * | 2019-11-26 | 2023-04-25 | 福州大学 | Method for reducing content of heterocyclic amine in dried pork slices by using cane molasses extract |
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