CN102516011A - Extraction method for lycopene - Google Patents
Extraction method for lycopene Download PDFInfo
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- CN102516011A CN102516011A CN2011104228121A CN201110422812A CN102516011A CN 102516011 A CN102516011 A CN 102516011A CN 2011104228121 A CN2011104228121 A CN 2011104228121A CN 201110422812 A CN201110422812 A CN 201110422812A CN 102516011 A CN102516011 A CN 102516011A
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Abstract
The invention provides an extraction method for lycopene, comprising the following steps of: cleaning tomatoes, mincing, crushing, filtering, removing impurities and freeze-drying; leaching lycopene by the wall-breaking technology of a low-temperature ultrahigh-pressure cytometer with the assistance of an organic solvent; and performing reduced pressure concentration and freezing crystallization on the extract to obtain a lycopene crystal. The extraction method for lycopene provided by the invention is simple in process, short in extraction time, high in extraction efficiency, low in cost, high in the purity of greater than 98% of lycopene crystal and suitable for industrialized production.
Description
Technical field
The invention belongs to field of food industry, particularly a kind of process for extracting of natural lycopene.
Background technology
Lyeopene (Lyeopene) is a kind of natural carotenoid, owing to be from tomato, to separate to make the earliest, claims Lyeopene.Lyeopene molecular formula C
40H
56, molecular weight 536.85.Lyeopene is soluble in chloroform, benzene, dissolves in ether, sherwood oil, hexane, acetone, is insoluble in methyl alcohol, and is water insoluble.Lyeopene can be removed oxyradical; Its antioxidant property is the strongest in all carrotenoid; The ability of its removing singlet oxygen is 100 times of antioxidant vitamin E commonly used, is more than 2 times of β-Hu Luobusu, has effects such as the cell proliferation of inhibition, increase immunizing power in addition.In recent years, the exploitation of Lyeopene related prods has become a focus of functional foodstuff and new drug research in the world.From world wide, lycopene product is mainly used in industries such as foodstuff additive, natural colorant, makeup.
Lyeopene mainly is present in fruits such as tomato, watermelon, Radix Dauci Sativae, strawberry, grape and citrus and the red plam oil; Wherein the content in the tomato is the highest; Generally can reach 3-14mg/100g, and content different and different with kind and ripening degree, ripening degree is high more; The content of Lyeopene is also high more, reaches as high as more than every hectogram 400mg.Tomato red in the tamato fruit have two kinds of existences: major part is present in the chromoplastid with elongated, acicular crystallized form; Sub-fraction (about 10%) then is present in the cell with protein formation complex body in addition, therefore needs fracturing cell walls to discharge Lyeopene.
At present, the process for extracting of Lyeopene mainly contains methods such as solvent extraction method, enzyme hydrolysis method, saponification method, microwave extraction method, ultrasonic extraction, supercritical extraction, chemical synthesis.Thereby traditional solvent extraction method is through the permeability that changes cell walls or film inner mixture to be infiltrated selectively; And Lyeopene is a fat-soluble pigment; Organic solvent is difficult for cell walls and the cytolemma that material is passed in infiltration, can not be well with extract stripping from organoid, so solvent extraction method technology is simple; Treatment capacity is big, but extraction time is long, extraction efficiency is not high.On this basis, adopt low-temperature ultrahigh-pressure cytoclasis appearance assisted extraction, the smudge cells releasing product shortens extraction time quickly and efficiently, improves extraction efficiency, and low, the less investment of cost is a kind of efficient ways in the industrial production.
Summary of the invention
Problem to be solved by this invention is the problem that conventional solvent extraction method extraction time is long, extraction efficiency is low; On solvent lixiviate basis, combine utilization low-temperature ultrahigh-pressure cytoclasis appearance broken wall to discharge content; Can improve extraction efficiency, shorten extraction time, effectively reduce cost.
To achieve these goals, the present invention adopts following technical scheme:
A kind of lycopene method of from tomato, extracting may further comprise the steps:
1) pre-treatment of tomato
Tomato is clean, chopping, broken in historrhexis's machine; The centrifugal tomato-sauce that gets of 6000～8000r/min; Add isopyknic absolute ethyl alcohol and stirring 5～10min and remove the lipophilic substance of pure dissolubility; The centrifugal again upper strata ethanol of abandoning, the gained deposition is carried out vacuum lyophilization, makes moisture content less than 10%.
2) preparation of Lyeopene bullion
Thing to be extracted is added in the reaction kettle, and (acetone: normal hexane=1: 1～3: 1), solid-liquid ratio is 1: 2～1: 5 to add organic solvent; Mix, broken with low-temperature ultrahigh-pressure cytoclasis appearance, 4～6 ℃ of service temperatures; WP is 250～350Mpa; Through the disposable fragmentation of 35～55s, centrifugal collection supernatant, underpressure distillation concentrates can get the Lyeopene bullion.
3) Lyeopene is refining
The Lyeopene bullion adds behind an amount of absolute ethyl alcohol at-20 ℃ of following freezing and crystallizings, uses low-temperature anhydrous washing with alcohol twice behind the centrifugal crystal again, drying, get final product purity greater than 98% lycopene crystal.
Owing to contain a large amount of moisture in the tomato pomace, organic solvent is difficult to get in the cell, and extraction effect is very poor, and therefore raw material is carried out processed can improve extraction yield.Common dewatering type is to adopt ethanol dehydration, and the rate that dewaters is lower, and the mass consumption ethanolic soln can only be removed the moisture about 30%, or adopts the mode of heat drying to dewater, but heating is unfavorable for the stable of Lyeopene.Adopt vacuum lyophilization to dewater among the present invention, kept the product biological activity, when the moisture removal rate reaches 90% when above, extraction effect is untreated about 100 times.
In cytoclasis, the ultrasonic disruption method is comparatively commonly used at present, but the broken cost of ultrasonication is high, is prone to cause the violent rising of temperature, in, during large-scale operation, the chemical radical of generation can make some susceptibility active substance inactivation.By contrast; Low-temperature ultrahigh-pressure continuous flow cell crusher is to utilize the ultra-high voltage energy to make sample pass through the slit abrupt release; Under the effect of shearing effect, hole effect, collision effect, make cytoclasis, whole process is carried out in 4 ℃～6 ℃ cold cycle water-bath; Keep original material active, have the potentiality of suitability for industrialized production.
The invention has the beneficial effects as follows:
1) adopt the vacuum lyophilization mode that tomato-sauce is carried out pre-treatment, dehydrating effect is good, improves extraction yield effectively;
2) adopt the auxiliary organic solvent extraction Lyeopene of low-temperature ultrahigh-pressure cytoclasis, weak point consuming time, extraction yield is high, obtains lycopene crystal purity greater than 98%, reaches extraction effect preferably.
Description of drawings
Accompanying drawing is that tomato is cleaned, after the chopping, fragmentation, filtration, removal of impurities, lyophilize, adopt low-temperature ultrahigh-pressure cell instrument wall breaking technology to assist the organic solvent lixiviate, the process flow sheet that extracting solution must lycopene crystal behind concentrating under reduced pressure, freezing and crystallizing.
Embodiment
Following instance is used to explain the present invention, but content of the present invention is not limited thereto.
Embodiment 1
The 10kg tomato is clean, chopping, broken in historrhexis's machine, slurries are with the centrifugal 10min of 6000rpm, and abandoning supernatant adds absolute ethyl alcohol again and carries out the alcohol processing, removes the lipophilic substance of a large amount of pure dissolubilities, must precipitate after the filtration.Throw out is carried out vacuum lyophilization, carry out processed, making moisture content is 8%.Thing to be extracted is added in the reaction kettle, and (acetone: normal hexane=1: 1), solid-liquid ratio is 1: 2 to add organic solvent; Temperature is 30 ℃; Carry out low-temperature ultrahigh-pressure cytoclasis, WP is 280Mpa, under 4～6 ℃ through the disposable fragmentation of 55s; Filter the back and obtain extracting solution, extracting solution is carried out underpressure distillation can get the Lyeopene bullion; The Lyeopene bullion adds behind an amount of absolute ethyl alcohol at-20 ℃ of following freezing and crystallizings, uses low-temperature anhydrous washing with alcohol twice behind the centrifugal crystal again, drying, get final product the lycopene crystal 0.35g of purity 98.24%.
Embodiment 2
The 20kg tomato is clean, chopping, broken in historrhexis's machine, slurries are with the centrifugal 5min of 7000rpm, and abandoning supernatant adds absolute ethyl alcohol again and carries out the alcohol processing, removes the lipophilic substance of a large amount of pure dissolubilities, must precipitate after the filtration.Throw out is carried out vacuum lyophilization, carry out processed, make moisture content less than 10%.Thing to be extracted is added in the reaction kettle, and (acetone: normal hexane=2: 1), solid-liquid ratio is 1: 3 to add organic solvent; Temperature is 35 ℃; Carry out low-temperature ultrahigh-pressure cytoclasis, WP is 300Mpa, under 4～6 ℃ through the disposable fragmentation of 45s; Filter the back and obtain extracting solution, extracting solution is carried out underpressure distillation can get the Lyeopene bullion; The Lyeopene bullion adds behind an amount of absolute ethyl alcohol at-20 ℃ of following freezing and crystallizings, uses low-temperature anhydrous washing with alcohol twice behind the centrifugal crystal again, drying, get final product the lycopene crystal 0.74g of purity 98.73%.
Embodiment 3
The 50kg tomato is clean, chopping, broken in historrhexis's machine, slurries are with the centrifugal 3min of 8000rpm, and abandoning supernatant adds absolute ethyl alcohol again and carries out the alcohol processing, removes the lipophilic substance of a large amount of pure dissolubilities, must precipitate after the filtration.Throw out is carried out vacuum lyophilization, carry out processed, make moisture content less than 10%.Thing to be extracted is added in the container, and (acetone: normal hexane=3: 1), solid-liquid ratio is 1: 5 to add organic solvent; Temperature is 45 ℃; Carry out low-temperature ultrahigh-pressure cytoclasis, WP is 350Mpa, under 4～6 ℃ through the disposable fragmentation of 40s; Filter the back and obtain extracting solution, extracting solution is carried out underpressure distillation can get the Lyeopene bullion; The Lyeopene bullion adds behind an amount of absolute ethyl alcohol at-20 ℃ of following freezing and crystallizings, uses low-temperature anhydrous washing with alcohol twice behind the centrifugal crystal again, drying, get final product the lycopene crystal 1.78g of purity 98.56%.
Claims (7)
1. one kind is extracted lycopene method from tomato, it is characterized in that, may further comprise the steps:
(1) pre-treatment of tomato: with tomato clean, chopping, broken in historrhexis's machine, cross and filter tomato-sauce, tomato-sauce removal of impurities, dehydration;
(2) preparation of Lyeopene bullion: combine with organic solvent lixiviate and low-temperature ultrahigh-pressure breaking cell wall mode and to extract Lyeopene, the underpressure distillation extracting solution, the Lyeopene bullion;
(3) Lyeopene is refining: add the absolute ethyl alcohol low temperature crystallization, washing, drying get final product lycopene crystal.
2. method according to claim 1 is characterized in that, the mode of removal of impurities is in the described step (1): add isopyknic absolute ethyl alcohol to tomato-sauce, stir the lipophilic substance that 5～10min removes by filter pure dissolubility.
3. method according to claim 1 is characterized in that, the throw out in the described step (1) after the removal of impurities dewaters with the mode of vacuum lyophilization.
4. method according to claim 1 is characterized in that, organic solvent is the mixture of acetone and normal hexane in the described step (2), and acetone: normal hexane=1: 1～3: 1, solid-liquid ratio are 1: 2～1: 5.
5. method according to claim 1 is characterized in that, adopts the broken tomato cell walls of low-temperature ultrahigh-pressure cytoclasis appearance in the described step (2), and 4～6 ℃ of service temperatures, WP are 250～350Mpa, the disposable fragmentation of 35～55s.
6. method according to claim 1 is characterized in that, the temperature of low temperature crystallization is-15 ℃～-20 ℃ in the described step (3).
7. method according to claim 1 is characterized in that lycopene crystal purity is greater than 98%.
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| CN2011104228121A CN102516011A (en) | 2011-12-16 | 2011-12-16 | Extraction method for lycopene |
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| CN2011104228121A CN102516011A (en) | 2011-12-16 | 2011-12-16 | Extraction method for lycopene |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106512470A (en) * | 2016-11-16 | 2017-03-22 | 揭阳市腾晟科技咨询有限公司 | Pretreatment method for extracting effective ingredients of plant |
| CN106954832A (en) * | 2017-05-17 | 2017-07-18 | 安徽华禹食品有限公司 | A kind of antimicrobial form sesame paste and preparation method |
| CN107495372A (en) * | 2017-09-27 | 2017-12-22 | 广州市范乐医药科技有限公司 | A kind of preparation method of Xanthophyll micro-capsule |
| CN107602641A (en) * | 2017-08-07 | 2018-01-19 | 武汉爱民制药股份有限公司 | A kind of method based on freeze-drying ultra high pressure extraction otoginsenoside from buckeye |
| CN110710662A (en) * | 2019-11-20 | 2020-01-21 | 合肥工业大学 | Processing technology of tomato powder rich in cis-lycopene |
-
2011
- 2011-12-16 CN CN2011104228121A patent/CN102516011A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106512470A (en) * | 2016-11-16 | 2017-03-22 | 揭阳市腾晟科技咨询有限公司 | Pretreatment method for extracting effective ingredients of plant |
| CN106954832A (en) * | 2017-05-17 | 2017-07-18 | 安徽华禹食品有限公司 | A kind of antimicrobial form sesame paste and preparation method |
| CN107602641A (en) * | 2017-08-07 | 2018-01-19 | 武汉爱民制药股份有限公司 | A kind of method based on freeze-drying ultra high pressure extraction otoginsenoside from buckeye |
| CN107602641B (en) * | 2017-08-07 | 2019-12-17 | 武汉爱民制药股份有限公司 | Method for extracting aescine from buckeye seeds under ultrahigh pressure based on freeze drying |
| CN107495372A (en) * | 2017-09-27 | 2017-12-22 | 广州市范乐医药科技有限公司 | A kind of preparation method of Xanthophyll micro-capsule |
| CN110710662A (en) * | 2019-11-20 | 2020-01-21 | 合肥工业大学 | Processing technology of tomato powder rich in cis-lycopene |
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Application publication date: 20120627 |