CN102515954A - Special biological organic fertilizer for verticillium wilt and production method for same - Google Patents
Special biological organic fertilizer for verticillium wilt and production method for same Download PDFInfo
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Abstract
The invention provides a special biological organic fertilizer for verticillium wilt, formed by adsorbing biocontrol bacillus subtilis NCD-2, bacillus mucilaginosus krassilnikov and bacillus megaterium by an adsorbing medium, mixing in a mass ratio of (6 to 4): (3 to 2): (3 to 2) to obtain a compound microbial inoculum, and then mixing with hydrolysis residue. The invention further provides a production method for the biological organic fertilizer. The special biological organic fertilizer for verticillium wilt provided by the invention can remarkably improve the effect of biological fertilizer, has obvious prevention and treatment effects on the diseases of crop, greatly reduces seedling disease rate, as well as can comprehensively provide the nutrients needed by the growth of crop and increase the yield of crop.
Description
Technical field
The present invention relates to a kind of bio-organic fertilizer, and the working method of this bio-organic fertilizer, specifically, relate to a kind of bio-organic fertilizer with biocontrol effect, and the working method of this fertilizer.
Background technology
The insect pest of farm crop, disease are the important factor that influences its output all the time, disease and pest make the serious underproduction of farm crop, even total crop failure, therefore, extremely important to effective control of disease and pest.China's agricultural serves as the main prevention and elimination of disease and pests that comes with chemical pesticide, chemical bactericide for a long time, and over nearly 20 years, important effect has been brought into play in using to the safety in production that ensures China's agricultural of chemical pesticide; But cause the anti-tonality of agricultural tillage to reduce thus with intensive; Be too dependent on chemical fertilizer and agricultural chemicals for a long time, cause the waste of a large amount of Nonrenewable energy resources, the farmland soil property degenerates; Fertility descends, and crop products is residual, quality reduction and food and environmental pollution problems become increasingly conspicuous.At present China's unit surface chemical fertilizer usage quantity is that (data in recent years shows for the U.S. and world average level 1.6 times; The usage quantity of nitrogenous fertilizer has reached 3.8 times of world average level); Annual total consumption is 1.4 hundred million tons (4,240 ten thousand tons of folding nutrients); China's chemical fertilizer ultimate production had increased by 90.7% in nearest 10 years; And total output of grain has only increased by 9.1%, and the rate of loss of chemical nitrogen fertilizer is up to 70%, and this is wasted with a large amount of energy more than 1,000 hundred million yuan and causes severe environmental pollution with regard to meaning to be lost in every year.Unreasonable fertilising causes groundwater azotate pollution, causes the quality and yield of agricultural byproducts to descend, and Beijing's spinach nitrate content is up to 2358mg/Kg; Radish 2177mg/Kg; Shanghai, Guangzhou vegetables nitrite exceed standard 2-8 doubly, and nitrite exceeds standard 12 times unexpectedly in the root, stem and leaf vegetables of Linyi, Shandong, and the Qingdao City inspects by random samples vegetables in 25; The recall rate of nitrate salt is 100%, exceeds standard 84%.
When reducing the chemical pesticide usage quantity, the microbial-bacterial fertilizer of developing biological pesticide or having a biological and ecological methods to prevent plant disease, pests, and erosion characteristic has become a kind of international trend.Relevant expert finds that in research and development a lot of mikrobes and meta-bolites thereof have the dual-use function of biological and ecological methods to prevent plant disease, pests, and erosion and nutrition concurrently both at home and abroad; Maybe the microbiobacterial agent that has nutrition and biological and ecological methods to prevent plant disease, pests, and erosion is respectively had after composite significantly anti-ly with diseases prevention and short effect such as long, this exploitation that is the microbial-bacterial fertilizer with biological and ecological methods to prevent plant disease, pests, and erosion characteristic provides thinking and reference.The biological and ecological methods to prevent plant disease, pests, and erosion factor that is applied to soil-borne disease of report is a lot of in the world; Comprise fungi, bacterium, viral phage and defence line bacterium etc.; Wherein portioned product is used for commercialization production, and in integrated pest prevention and control and agricultural sustainable development, is bringing into play more and more important effect.
Cotton is a kind of important cash crop, is the natural matter of textile industry.At present, cotton verticillium wilt is in the cotton disease output of cotton to be threatened one of maximum disease, is a kind of soilborne vascular bundle diseases, and serious economy loss has been caused in the main cotton producing region that this kind disease has been found everywhere through the world.Life-time service chemical fertilizer, agricultural chemicals etc. not only cause seriously hardening of soil to influence the output of cotton, but also can cause the decline of cotton quality in addition, thereby influence cotton grower's income.
Utilizing microbial control is present Plant diseases; Particularly soil passes one of effectively preventing measure of Plant diseases; Yet when reducing the chemical pesticide usage quantity; Develop drug effect with biological pesticide and the bio-feritlizer that has fertilizer efficiency concurrently and become a kind of international trend; Relevant both at home and abroad expert finds that under study for action a lot of mikrobes have remarkable inhibiting bacterial strain to cotton verticillium wilt, yet antibacterial bacterial strain is combined with the nutrition bacterial strain, develops that to have multi-functional bacterial manure comparatively rare in the market.
Summary of the invention
Therefore, the technical problem that the present invention will solve provides a kind of bio-organic fertilizer that verticillium is had good biological and ecological methods to prevent plant disease, pests, and erosion property effect, and said bio-organic fertilizer quality meets " biological organic fertilizer " agriculture standard (NY884-2004); Second technical problem that the present invention will solve provides the working method of this bio-organic fertilizer.
Technical scheme of the present invention is, a kind of verticillium bio-organic fertilizer special material comprises raw material and microbial inoculum, and said raw material is the hydrolysis residue of amino acid production process; Said microbial inoculum is by biological and ecological methods to prevent plant disease, pests, and erosion property subtilis (Bacillus subtilis) NCD-2; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); Bacillus megaterium (Bacillus megaterium), the mass ratio of pressing 6-4: 3-2: 3-2 with adsorption medium absorption back mixes; The mass ratio of said raw material and microbial inoculum is 50-96: 4-6.
Above-mentioned bacillus subtilis strain NCD-2 is by plant protection institute of Hebei province's agricultural AS; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC); Preserving number: 1019; The visible application number of concrete proterties: 200310109619.8, the open date is on September 15th, 2004.
According to verticillium bio-organic fertilizer special material of the present invention, preferably, the living bacteria count amount of said complex micro organism fungicide reaches 9.5 * 10
8Individual/more than the gram.
Preferably, said bio-organic fertilizer also can comprise one or more in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, the trace element fertilizer.
The present invention also provides a kind of working method of verticillium bio-organic fertilizer special material, comprises the preparation of microbial inoculum, and this method may further comprise the steps:
(1) processing of amino acid hydrolysis residue: break the bulk hydrolysis residue into pieces powdered or small-particle,, pulverize, let it fully react the compound stacking, make its pH reach 5.0-8.0 with the alkaline matter proportional mixing;
(2) preparation of complex micro organism fungicide: with biological and ecological methods to prevent plant disease, pests, and erosion property subtilis (Bacillus subtilis) NCD-2; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); After fermenting separately, bacillus megaterium (Bacillus megaterium) bacterial classification adsorbs with adsorption medium; Again according to 6-4: the mass ratio of 3-2: 3-2 mixes, and pulverizes;
(3) preparation of bio-organic fertilizer: the microbiobacterial agent that hydrolysis residue that step (1) is obtained and step (2) obtain mixes, and pulverize the back that stirs, and obtains the powdery bio-organic fertilizer; The mass ratio that said complex micro organism fungicide accounts for the refined biometric organic fertilizer is 4-6%.
According to the working method of verticillium bio-organic fertilizer special material of the present invention, preferably, also can add in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, the trace element fertilizer one or more in the said step (3).Nitrogenous fertilizer, potash fertilizer and phosphate fertilizer can add according to concrete needs, and wherein the main raw material of N has: urea, ammonium sulfate, an ammonium nitrate etc.; P
2O
5Main raw material have: monoammonium phosphate, diammonium phosphate, superphosphate of lime and calcium magnesium phosphorus; K
2The main raw material of O has: Repone K and vitriolate of tartar etc.
Further, can add trace element fertilizer at biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilizer material according to the nutrient requirement of client to fertilizer.Trace element in also can adding: magnesium is used sal epsom; Calcium is used nitrocalcite; Manganese is used manganous sulfate; Zinc is used zinc sulfate; Boron is used borax; Copper is used copper sulfate, and iron is with ferrous sulfate etc.
According to the working method of verticillium bio-organic fertilizer special material of the present invention, preferably, said alkaline matter is a kind of in lime, Pottasium Hydroxide and the sodium hydroxide.
Further, said lime adds according to the mass ratio of hydrolysis residue: lime=4-5: 0.5-1.
For finally to make the pH of hydrolysis residue reach 5.0-8.0, the general add-on of lime is mixed for the mass ratio according to hydrolysis residue: lime=4-5: 0.5-1 in the hydrolysis residue described in the above-mentioned steps (1) and lime blended ratio.After hydrolysis residue and lime pile fully reacted, temperature dropped to normal temperature and gets final product, and the preferred reaction times is 8-20 hour.
In the preparation of above-mentioned microbial inoculum, the adsorption medium that the bacterium liquid behind the strain fermentation uses is as this area medium commonly used, like the peat composed of rotten mosses, powdered rice hulls etc.The absorption ratio is to make the viable count of mix bacterium agent finally reach 9.5 * 10
8Individual/more than the gram.The water cut of complex micro organism fungicide remains in 30%.
Preferably, pulverize requirement described in step (2) and the step 3 and reach the 40-100 order for fineness, the best is the 60-80 order.
Further, as shown in table 1, the organic content of handling the posthydrolysis slag has reached 30-32; Meet the requirement of organic content in " biological organic fertilizer " industry standard (NY884-2004) >=25%; If want to produce the more biological organic fertilizer of high organic content, can add organic matter material in process of production, to improve content organic in the product; Concrete prescription obtains according to the content of organic matter material and the technical indicator cubage of product; High-quality organic matter material commonly used has: the peat composed of rotten mosses, humic acids etc., also can start from cost consideration, and select other organic matter materials.
Requirement according to national agricultural microorganism microbial inoculum standard (GB 20287-2006) detects standard-required living bacteria count>=2 * 10 to biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide (pulvis)
8Individual/gram, assorted bacterium rate≤20%, mould bacterium number≤3 * 10 of mixing
6Individual/gram, the pH value is 5.5-8.5.
Table 2: the technical indicator that adopts the biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide of different ingredients production
Requirement and detection method according to " biological organic fertilizer " industry standard (NY884-2004) detect product, living bacteria count in the standard-required specification product>=2 * 10
7Individual/gram, organic>=25%, the pH value is 5.5-8.5, and moisture content≤30% proves through a large amount of different tests, and heavy metal content does not surpass the innoxious index of Ministry of Agriculture's bio-feritlizer industry standard.
Table 3: adopt different ingredients to produce the technical indicator of biological organic fertilizer
Above-mentioned individual/gram is meant the number that contains mikrobe in every gram solid; % is meant the massfraction that adds and contain target compound in the solid of per hundred parts of quality.
Subtilis NCD-2 of the present invention; The number of viable of the liquid fermentation liquid that is developed into is greater than 10,000,000,000/gram; All have good control effect for germs such as cotton verticillium wilt, cotton wilt, wheat scab, ring rot of apple bacterium, control rate reaches more than 67%.
After the nutrition bacterial strain bacillus megaterium of the above-mentioned biocontrol strain that screens and phosphorus decomposing, potassium decomposing and colloid bacillus cereus cooperated, produce a kind of complex micro organism fungicide, this microbiobacterial agent is with reference to main standard index both at home and abroad; Through optimizing proportioning; Give full play to the multi-function action of composite bacterial manure, do not have antagonistic action each other, make living bacteria count reach maximum; The biological organic fertilizer that utilizes this complex micro organism fungicide to produce can supply fertile to farm crop; Can draw to crop rhizosphere soils again and be implanted with the beneficial microorganism dominant group, effectively prevent the generation of soil-borne disease of crop, reach the purpose of the strong sprout of fertilizing the soil, short biological and ecological methods to prevent plant disease, pests, and erosion disease and good quality and high output.
Tunning hydrolysis residue in the glutamate production process (hydrolysis filter cake) can't effectively utilize and cause a large amount of overstocking because it is by force acid; Lose or abandon in soil and can cause the water quality soil acidification to pollute with current; Country forbids to discharge; But through detecting, hydrolysis residue except that acidity by force, wherein contain great number of organic matters, amino acid, humic acid and npk nutrient, glucide and trace element; These nutritive substances can provide microorganism growth required nutrient; Not only can not influence the viable bacteria survival in the biological organic fertilizer with hydrolysis residue as the carrier of biological organic fertilizer, and use the back and under the suitable soil moisture and moisture, can promote the growth and breeding of useful microbe in soil in the fertilizer, improve nearly one times as the biological organic fertilizer viable bacteria reproduction speed of starting material production than the use peat composed of rotten mosses.Raw material as a kind of good biological organic fertilizer can add above-mentioned complex micro organism fungicide in hydrolysis residue, produce the biological organic fertilizer that obtains having biocontrol effect.
The biological and ecological methods to prevent plant disease, pests, and erosion property complex micro organism fungicide quality of the present invention's development meets the requirement (GB20287-2006) of national agricultural microorganism microbial inoculum; The biological and ecological methods to prevent plant disease, pests, and erosion property biological organic fertilizer product of producing meets " biological organic fertilizer " agriculture standard (NY884-2004) fully; Effectively solve the technical problem that biological and ecological methods to prevent plant disease, pests, and erosion property biological organic fertilizer faces at present; Remedy deficiency of the prior art; Working method a kind of cheapness, high, solubility biological organic fertilizer environmental protection, that have the agricultural chemicals effect concurrently of resource utilization is provided, makes simultaneously and produce the amino acid hydrolysis residue that produces in the monosodium glutamate process and reach disposal of resources and effectively utilize.
Compared with prior art, the invention has the advantages that:
1, make full use of cotton verticillium wilt is had remarkable inhibiting biocontrol strain, and with its effect extension, compound with the nutrition bacterial strain, produce the biological and ecological methods to prevent plant disease, pests, and erosion property fertilizer that has diseases prevention and fertilizer efficiency concurrently;
2, filtered out good organic carrier, made it be more conducive to diseases prevention bacterial strain deciding in soil and grow, its effect of performance that more can be permanent than bacterium liquid;
3, be the basis with the Agro-ecology balance, effectively compound through biocontrol microorganisms and fixed nitrogen, phosphorus decomposing, potassium solubilizing bacteria, instead of part chemical pesticide and fertilizer, controlling crop diseases and insect pests promotes plant growth, reaches the combination of drug effect of fertilizer efficiency and the biological pesticide of bio-feritlizer;
4, after using, biological and ecological methods to prevent plant disease, pests, and erosion property bio-feritlizer has diseases prevention and fertilizer efficiency effect concurrently; Can improve crop disease-resistant property, and, keep plant growth healthy and strong the absorption and the utilization ratio of fertilizer; Also can promote the breeding of biocontrol microorganisms to improve the crop disease-resistant ability conversely, and help to form good Agro-ecology system.
Description of drawings
Fig. 1 is the manufacturing process flow diagram of amino acid hydrolysis residue.
Fig. 2 is the preparation flow figure of complex micro organism fungicide of the present invention.
Fig. 3 is the preparation flow figure of powdery verticillium bio-organic fertilizer special material.
Fig. 4 is the influence column diagram of different treatment to the cotton verticillium wilt sickness rate.
Embodiment
One, produces biological organic fertilizer with the amino acid hydrolysis residue
Embodiment 1-1
1, the preparation of complex micro organism fungicide: with biological and ecological methods to prevent plant disease, pests, and erosion property subtilis NCD-2; Colloid bacillus cereus; Three kinds of bacterial classifications of bacillus megaterium separately the bacterium liquid after the fermentation with the peat composed of rotten mosses in bacterium liquid: the ratio of the peat composed of rotten mosses=1: 4 is adsorbed; Absorption back solid, powdery microbial inoculum was according to 6: 2: 2 mixed, and the living bacteria count amount of mixing the back composite fungus agent reaches 13 * 10
8Individual/more than the gram, moisture remains in 30%.
2, sampling.The hydrolysis residue that produces in the amino acid production process is taken from Henan "Lotus" gourmet powder company limited by shares;
3, pH conditioning: break the bulk hydrolysis residue into pieces powdered or small-particle, with powdered lime by hydrolysis residue: lime=mixes at 4: 1, with chain kibbler pulverizing stacking afterwards 8-12 hour, let it fully react, be cooled to then pulverize behind the normal temperature;
4, the essential property of hydrolysis residue is measured, heavy metal content is not measured well below the industry standard (seeing table 1) of biological organic fertilizer;
Table 4: the basic proterties of handling the posthydrolysis slag
In stirrer, add and slowly add the powdery hydrolysis residue, and add the complex micro organism fungicide for preparing by weight 4% ratio, stirrer stirred 3-5 minute, made the material thorough mixing even.Make the fineness of material reach the 60-80 order through kibbler.Packing, metering, warehouse-in.
Embodiment 1-2
Except following technology, other make the biological and ecological methods to prevent plant disease, pests, and erosion property biological organic fertilizer of producing with the amino acid hydrolysis residue as embodiment 1-1.
The blending ratio of hydrolysis residue and powdered lime is a hydrolysis residue: lime=5: 1.
With biological and ecological methods to prevent plant disease, pests, and erosion property subtilis NCD-2; Colloid bacillus cereus; Each bacterial classification of bacillus megaterium separately the bacterium liquid after the fermentation with the peat composed of rotten mosses in bacterium liquid: the ratio of the peat composed of rotten mosses=1: 3 is adsorbed; Mix after the single microbial inoculum of absorption back solid, powdery, blending ratio is 4: 3: 3, and the living bacteria count amount of mixing the back composite fungus agent reaches 9.5 * 10
8Individual/more than the gram, moisture remains on 22-25%.
Batching: in stirrer, add and slowly add the powdery hydrolysis residue, and add the complex micro organism fungicide for preparing by weight 6% ratio.
Embodiment 1-3
Except following technology, other make the biological and ecological methods to prevent plant disease, pests, and erosion property biological organic fertilizer of producing with the amino acid hydrolysis residue as embodiment 1-1.
The blending ratio of hydrolysis residue and powdered lime is a hydrolysis residue: lime=5: 1.
With biological and ecological methods to prevent plant disease, pests, and erosion property subtilis NCD-2; Colloid bacillus cereus; Each bacterial classification of bacillus megaterium separately the bacterium liquid after the fermentation with the peat composed of rotten mosses in bacterium liquid: the ratio of the peat composed of rotten mosses=1: 3 is adsorbed; Mix after the single microbial inoculum of absorption back solid, powdery, blending ratio is 5: 2.5: 2.5, and the living bacteria count amount of mixing the back composite fungus agent reaches 10.5 * 10
8Individual/more than the gram, moisture remains on 22-25%.
Batching: in stirrer, add and slowly add the powdery hydrolysis residue, and add the complex micro organism fungicide for preparing by weight 5% ratio.
Comparative example 1 usefulness amino acid hydrolysis residue production does not have the biological organic fertilizer of biocontrol effect
Except following technology, other are as embodiment 1-1
The preparation of complex micro organism fungicide: with subtilis (Bacillus subtilis); Bacillus megaterium (Bacillus megaterium) and Bacillus mucilaginosus (Bacillus mucilagimosus krassilm) be fermentative prodn separately; Bacterium liquid after the fermentation with 80 order powdered rice hulls in bacterium liquid: the ratio absorption of powdered rice hulls=1: 4; Single microbial inoculum after three kinds of absorption is pulverized according to 1: 1: 1 mixed, made the total viable count that mixes back three bacterial classifications reach 4.8 * 10
8Individual/gram/gram more than, moisture remains in 30%.
Batching: in stirrer, add and slowly add the powdery hydrolysis residue, and add the complex micro organism fungicide for preparing by weight 6% ratio.
Comparative example 2 usefulness peat composed of rotten mosses production biological and ecological methods to prevent plant disease, pests, and erosion property biological organic fertilizers
Except following technology, other make the biological organic fertilizer of producing with the peat composed of rotten mosses as embodiment 1-1.
1, sampling.The peat composed of rotten mosses comes from Linfen, Shanxi, and basic proterties is seen table 5:
Table 5: the basic proterties of the peat composed of rotten mosses
2, batching.In stirrer, add and slowly add the powdery hydrolysis residue, and add the complex micro organism fungicide for preparing by weight 5% ratio.
Test Example
Cotton seedling pot experiment effect
Test is handled:
Handle 1 (ck1), blank is not promptly inoculated any microorganism strains in the soil;
Handle 2 (ck2), be contrast, hinder root inoculation cotton verticillium wilt germ; Pinch the root hair and the long young root of root of cotton seedling, inoculate 15ml bacterium liquid, place 18-28 ℃ greenhouse; 2d waters primary water, keeps certain humidity in order to morbidity, and inoculum size is 500-800 sporocyst/gram dry ground;
Handle 3, antimicrobial fluid regulation and control short of money are being handled on 2 bases, inoculation antagonism bacteria culture fluid 50ml/kg dry ground, antagonism bacterial concentration 10
7Cfu/ml;
Handle 4, the bio-organic fertilizer regulation and control are being handled on 2 bases, in the ratio adding bio-organic fertilizer of soil 1.5%;
Handle 5, contain antimicrobial bio-organic fertilizer regulation and control short of money, handling on 2 bases, in the biological and ecological methods to prevent plant disease, pests, and erosion property bio-organic fertilizer behind the ratio adding antagonism bacterium of soil 1.5%.
Each handles 7 basins, respectively the 10th, 20,40d, adopt crushing method sampling, do rhizosphere microflora and analyze counting; All the other 4 basins are respectively at 10d and 20d " Invest, Then Investigate ", statistics sickness rate.Plant 10d behind the seedling, insert pathogenic bacteria for the second time, continue to observe sickness rate, carry out microbiological analysis, counting.
Utilize the processing protection effect of antimicrobial fluid regulation and control short of money best behind the 1st the inoculation pathogenic bacteria, almost not morbidity, the antimicrobial substance in possible this stage nutrient solution is in the effect of performance bacteriostatic and disease prevention; Because comparing with other processing, the rhizospheric microorganism quantity of this processing do not preponderate; After the 2nd inoculation, sickness rate is 27%, maybe be because the antimicrobial substance colonization ability in the bacterium liquid is poor; Receive the influence of other pathogenic bacterias in the soil, quantity descend rapidly (inactivation or the utilization that is degraded);
Behind the 1st the inoculation pathogenic bacteria, be aided with antagonism bacteria organic fertilizer material and handle the processing more only inoculate pathogenic bacteria and compare, sickness rate has reduced by 32%; The processing of antimicrobial fluid regulation and control more short of money, sickness rate is obviously high, possibly be that the antagonism bacterium that is aided with in the antagonism bacteria organic fertilizer material does not also form dominant population at rhizosphere; But behind the 2nd the inoculation pathogenic bacteria, contain antagonism bacteria organic fertilizer material and handle the processing more only inoculate pathogenic bacteria and compare, sickness rate has reduced by 60%; Antimicrobial fluid regulation and control more short of money are handled sickness rate and have been reduced by 13%; The control effect of this processing is best, and the sickness rate that contains antimicrobial bio-organic fertilizer ability good restraining pathogenic bacteria short of money is described, bio-organic fertilizer has been carried good nutritional condition for the antagonism bacterium; The survival time of effective bacterium can be prolonged; Strengthen the antagonism bacterium rhizosphere decide to grow effect, be beneficial to auxiliary its and form dominant population, to reach best control effect.
Table 6: different treatment is to the influence of cotton verticillium wilt disease index
| Handle | Disease index (%) |
| The inoculation germ | 69.7 |
| Inoculation germ+antimicrobial fluid short of money is handled | 48.6 |
| Inoculation germ+bio-organic fertilizer is handled | 57.8 |
| Inoculation germ+contain antagonism bacteria organic fertilizer material to handle | 32.1 |
Can find out by table 6; Blank behind the inoculation germ is handled down, and the cotton seedling disease index is up to 69.7%; The disease index of three kinds of processing is followed successively by antagonism bacteria organic fertilizer material＜antimicrobial fluid processing＜compound manure processing short of money behind the inoculation germ, this shows that the disease index that applies for cotton seedling of antimicrobial fluid short of money has tangible reduction effect; And the treatment effect that the antagonism bacterium is added in the bio-organic fertilizer is higher than the effect that antagonism bacterial manure directly applies; Explain also that bio-organic fertilizer is brought into play in soil for the antagonism bacterium and suppress the cotton verticillium wilt effect booster action is clearly arranged, be beneficial to antagonism bacterium deciding in soil and grow, and form dominant population.
Table 7: bio-organic fertilizer is to the influence (* 10 of antagonistic bacterium at rhizosphere colonization
7The cfu/g dry ground)
Containing antimicrobial bio-organic fertilizer short of money is manured into soil and obviously helps the antagonism bacterium and decide to grow (table 7) at rhizosphere.After cultivating 10d; The quantity that contains cotton rhizosphere antagonistic bacterium in the antimicrobial bio-organic fertilizer processing short of money is 15.5 times that following antagonistic bacterium quantity is handled in antimicrobial fluid regulation and control short of money; And antagonistic bacterium shared ratio in rhizosphere bacteria also is higher than antimicrobial fluid regulation and control short of money far away to be handled, and becomes the dominant microflora in the bacterium.The back 40d of inoculation contains antimicrobial bio-organic fertilizer short of money and handles the antagonism bacterium to still have 3.47 * 10 in the quantity of rhizosphere
7The cfu/g dry ground accounts for 74% of rhizosphere bacteria sum, has very strong deciding to grow and the ability of the population of keeping on top, and the antagonism bacterium number of antimicrobial fluid short of money under handling has only 0.80 * 10
7The cfu/g dry ground accounts for 27.8% of total plate count.
Biocontrol microorganisms generally can play the effect of good restraining germ under laboratory condition, and in soil, uses the influence that receives extraneous factors such as temperature, humidity, soil pH value, pesticide residue or applying pesticides often, and a little less than the field planting ability, control effect is unstable.This experimental result shows identical trend; A little less than the antagonism bacterium colonization ability after antimicrobial fluid short of money is handled; Can not form dominant population, and after the antagonism bacterium is seeded in bio-organic fertilizer antagonistic bacterium had castering action clearly at the colonization ability of rhizosphere, the nutritive ingredient that is rich in the bio-organic fertilizer is beneficial to antimicrobial breeding short of money and growth; At the colonization ability of rhizosphere tangible enhancement is arranged for bacterium, be beneficial to the formation dominant population.
Simultaneously, find that this bacterial strain is not only to cotton verticillium wilt through other experiments, but also inhibited to germs such as cotton anthracnose, cotton wilt, wheat hypochnuss.
Claims (7)
1. a verticillium bio-organic fertilizer special comprises raw material and microbial inoculum, it is characterized in that: said raw material is the hydrolysis residue of amino acid production process; Said microbial inoculum is by biological and ecological methods to prevent plant disease, pests, and erosion property subtilis (Bacillus subtilis) NCD-2; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); Bacillus megaterium (Bacillus megaterium), the mass ratio of pressing 6-4: 3-2: 3-2 with adsorption medium absorption back mixes; The mass ratio of said raw material and microbial inoculum is 50-96: 4-6.
2. verticillium bio-organic fertilizer special according to claim 1 is characterized in that: the living bacteria count amount of said complex micro organism fungicide reaches 9.5 * 10
8Individual/more than the gram.
3. verticillium bio-organic fertilizer special according to claim 1 is characterized in that: said bio-organic fertilizer also comprises one or more in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, the trace element fertilizer.
4. the working method of a verticillium bio-organic fertilizer special comprises the preparation of microbial inoculum, it is characterized in that: this method may further comprise the steps:
(1) processing of amino acid hydrolysis residue: break the bulk hydrolysis residue into pieces powdered or small-particle,, pulverize, let it fully react the compound stacking, make its pH reach 5.0-8.0 with the alkaline matter proportional mixing;
(2) preparation of complex micro organism fungicide: with biological and ecological methods to prevent plant disease, pests, and erosion property subtilis (Bacillus subtilis) NCD-2; Colloid bacillus cereus (Bacillus mucilaginosus Krassilnikov); After fermenting separately, bacillus megaterium (Bacillus megaterium) bacterial classification adsorbs with adsorption medium; Again according to 6-4: the mass ratio of 3-2: 3-2 mixes, and pulverizes;
(3) preparation of bio-organic fertilizer: the microbiobacterial agent that hydrolysis residue that step (1) is obtained and step (2) obtain mixes, and pulverize the back that stirs, and obtains the powdery bio-organic fertilizer; The mass ratio that said complex micro organism fungicide accounts for the refined biometric organic fertilizer is 4-6%.
5. according to the working method of the said verticillium bio-organic fertilizer special of claim 4, it is characterized in that: be added with in nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, the trace element fertilizer one or more in the said step (3).
6. according to the working method of the said verticillium bio-organic fertilizer special of claim 4, it is characterized in that: said alkaline matter is a kind of in lime, Pottasium Hydroxide and the sodium hydroxide.
7. according to the working method of the said verticillium bio-organic fertilizer special of claim 6, it is characterized in that: said lime adds according to the mass ratio of hydrolysis residue: lime=4-5: 0.5-1.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641605A (en) * | 2013-11-26 | 2014-03-19 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN104098401A (en) * | 2014-07-29 | 2014-10-15 | 夏宝昌 | Composite microbe fertilizer and preparation method thereof |
| CN105367305A (en) * | 2015-12-24 | 2016-03-02 | 深圳市芭田生态工程股份有限公司 | Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer |
| CN106939290A (en) * | 2017-03-16 | 2017-07-11 | 河北省农林科学院植物保护研究所 | Bacillus subtilis HMB26553 and its application |
| CN108901507A (en) * | 2018-07-11 | 2018-11-30 | 新疆农垦科学院 | The method for preventing and treating cotton verticillium wilt |
| CN110668891A (en) * | 2019-09-25 | 2020-01-10 | 山东宝源生物科技股份有限公司 | Biocontrol type compound additive and application thereof in fertilizer |
| CN113527002A (en) * | 2021-04-29 | 2021-10-22 | 滨州市京阳生物肥业有限公司 | Preparation method of biological organic fertilizer for preventing and treating cotton verticillium wilt |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103641605A (en) * | 2013-11-26 | 2014-03-19 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN103641605B (en) * | 2013-11-26 | 2015-02-11 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN104098401A (en) * | 2014-07-29 | 2014-10-15 | 夏宝昌 | Composite microbe fertilizer and preparation method thereof |
| CN104098401B (en) * | 2014-07-29 | 2017-10-10 | 夏宝昌 | A kind of preparation method of compound microbial manure |
| CN105367305A (en) * | 2015-12-24 | 2016-03-02 | 深圳市芭田生态工程股份有限公司 | Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer |
| CN106939290A (en) * | 2017-03-16 | 2017-07-11 | 河北省农林科学院植物保护研究所 | Bacillus subtilis HMB26553 and its application |
| CN106939290B (en) * | 2017-03-16 | 2020-02-11 | 河北省农林科学院植物保护研究所 | Bacillus subtilis HMB26553 and application thereof |
| CN108901507A (en) * | 2018-07-11 | 2018-11-30 | 新疆农垦科学院 | The method for preventing and treating cotton verticillium wilt |
| CN110668891A (en) * | 2019-09-25 | 2020-01-10 | 山东宝源生物科技股份有限公司 | Biocontrol type compound additive and application thereof in fertilizer |
| CN113527002A (en) * | 2021-04-29 | 2021-10-22 | 滨州市京阳生物肥业有限公司 | Preparation method of biological organic fertilizer for preventing and treating cotton verticillium wilt |
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