CN102512442A - Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same - Google Patents
Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same Download PDFInfo
- Publication number
- CN102512442A CN102512442A CN2011104211239A CN201110421123A CN102512442A CN 102512442 A CN102512442 A CN 102512442A CN 2011104211239 A CN2011104211239 A CN 2011104211239A CN 201110421123 A CN201110421123 A CN 201110421123A CN 102512442 A CN102512442 A CN 102512442A
- Authority
- CN
- China
- Prior art keywords
- vitamin
- zinc
- oral administration
- emulsion formulation
- nanometer emulsion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a veterinary compound oral nano-emulsion containing zinc chelate, selenium and multivitamins and a preparation method for the same, wherein the veterinary compound oral nano-emulsion comprises the following components: zinc gluconate, a chelant, vitamin A, vitamin D3, vitamin E, sodium selenite, an emulsifier, an emulsifying aid, a preservative, an antioxidant and water. The veterinary compound oral nano-emulsion disclosed by the invention can comprehensively supplement vitamins; moreover, zinc can enhance esterase activity and thus promoting absorption for vitamin A, vitamin D can promote skeletal development, and selenium can promote absorption for vitamin E and enhance the antioxidation of vitamin E; and when toxic symptom appears due to excessive selenium, vitamin E can enhance excretion for selenium and decrease toxicity.
Description
Technical field
The present invention relates to a kind of chelated zinc, selenium and multivitamin animal compound oral administration nanometer emulsion formulation and preparation method thereof of containing, belong to the veterinary drug preparation technical field.
Background technology
Zinc is growth of animals or poultry, breed trace elements necessary.When the zinc in zinc deficiency in the feedstuff or the feedstuff was difficult for being absorbed by poultry, zinc deficiency will appear in poultry, causes the human body of poultry to be become thin, and crust thickens, plant raise, the obvious atrophy of testis of stud bird, oligospermia is grown, is bred all and be affected.Therefore, suitably zinc supplement in the livestock and poultry cultivation process.
Mostly the product of zinc supplement at present is inorganic zinc, and like zinc sulfate, zinc carbonate, zinc oxide etc., the zinc bioavailability of these inorganic states is lower.Therefore; Along with improving constantly of preparation level; Chelated zinc has replaced the inorganic zinc of difficult absorption gradually, and it is one type of chelate with unique circulus, integrates aminoacid and zinc element; Be to have form and the similar zinc element additive of biological function in a kind of and the animal body, mainly type has zinc lysine, zinc methionine.Zinc-amino acid chelate has following two big advantages: (1) steady chemical structure is difficult for being combined into insoluble compound with other materials or being attracted on the insoluble colloid.(2) biological value is high, not only absorbs soon, and can reduce biochemical process, practices thrift energy i (in vivo) consumption.
Chinese patent file CN1467199A (application number 02136013.8) provides a kind of L-zn lysine chelated of processing with biochemical method; It can replenish necessary metallic element zinc of organism and lysine; This material is used to prevent and treat owing to zinc deficiency lacks the various diseases that lysine causes, and can make the body recovery that is in sub-health state to health status.
Zinc and other trace element such as vitamin, selenium etc. are all growth of animals or poultry, breed requisite trace element; At present, vitamin and selenium and other trace elements often adopt nano-emulsion technology preparation oral formulations.The nano-emulsion technology is a preparation technique emerging in this area, and it is that particle diameter is Thermodynamically stable, the isotropism of 1～100nm, transparent or semitransparent homodisperse system by spontaneous formation such as water, oil, surfactant and cosurfactants.
Therefore, the required zinc of poultry, selenium and various vitamin are prepared into nano-emulsion, can effectively improve each trace elements absorbed effect.But shortcomings such as the product existence that contains chelated zinc and vitamin, selenium and other trace elements of adopting existing nano-emulsion technology to make is separated out, deposition have influenced the product effect of bringing into normal play.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of chelated zinc, selenium, vitamin A, vitamin D of containing simultaneously is provided
3, vitamin E oral administration nanometer emulsion formulation and preparation method thereof.
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 50～90g, chelating agen 45～90g, vitamin A 4～14g, vitamin D
30.3～0.9g, vitamin e1 5～30g, sodium selenite 0.1～0.5g, emulsifying agent 150～350g, co-emulsifier 80～220g, antiseptic 0.1～2.0g, antioxidant 0.1～2.0g, water is settled to 1L.
Preferably, every liter of component is following:
Zinc gluconate 75～80g, chelating agen 60～85g, vitamin A 5～10g, vitamin D
30.4～0.7g, vitamin E 15～25g, sodium selenite 0.2～0.4g, emulsifying agent 200～300g, co-emulsifier 90～150g, antiseptic 0.2～0.5g, antioxidant 0.2～0.3g, water is settled to 1L.
Described chelating agen is a kind of or combination in lysine hydrochloride, lysine alkali, methionine, glycine, the arginine; Preferred lysine hydrochloride or methionine; Further preferred lysine hydrochloride.
Described emulsifying agent is a tween 80, Arlacel-80, a kind of or combination among polyoxyethylene hydrogenated Oleum Ricini RH-40, the polyoxyethylene castor oil EL-35, preferred tween 80 or polyoxyethylene castor oil EL-35.
Said co-emulsifier is a kind of or combination in isopropyl alcohol, propylene glycol, Polyethylene Glycol, the glycerol, preferred Polyethylene Glycol or isopropyl alcohol.
Described antiseptic is selected from ethyl hydroxybenzoate, methyl hydroxybenzoate or benzoic acid, preferred ethyl hydroxybenzoate;
Described antioxidant is 2,6-di-tert-butyl-4-methy phenol (BHT), Butylated hydroxyanisole (BHA), sodium thiosulfate; Preferred 2, the 6-di-tert-butyl-4-methy phenol.
The above-mentioned method for preparing that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc comprises the steps:
(1) take by weighing zinc gluconate, chelating agen in proportion, add in the suitable quantity of water, 20～40 ℃ of stirring and dissolving add sodium selenite again, treat the solution clarification after, through membrane filtration, chelated zinc solution;
(2) weighing vitamin A, vitamin D in proportion
3, vitamin E, add antiseptic, antioxidant, be stirred to that the dissolving back adds emulsifying agent fully, co-emulsifier stirs to clarification, temperature transfers to 20～40 ℃, slowly adds the zinc solution that closes that step (1) makes, water is settled to 1L, promptly gets.
Filter membrane in the said step (1) is 5 μ m filter membranes.
Vitamin A described in the present invention, vitamin D
3Be the vitamin that meets feedstuff national standard purity with vitamin E, room temperature is oily down.
Beneficial effect
1, the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc according to the invention vitimin supplement comprehensively; And zinc can strengthen esterase active and promote the absorption of vitamin A; Vitamin D can promote skeleton development, and selenium can promote the absorption of vitamin E, strengthens its antioxidation; When selenium is excessive when poisoning symptom occurring, vitamin E can increase selenic drainage, reduces toxicity; The compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc according to the invention is compared existing like product improvement amplitude and is reached more than 6%, sees the contrast test among the embodiment for details.
2, method for preparing of the present invention adopts unique chelated zinc preparation technology; The chelated zinc that this method makes can stable existence in nano-emulsion preparation; Chelated zinc and nano-emulsion can dual promotion zinc absorption; Simultaneously, the chelated zinc that the chelated zinc that adopts this method to make is produced than traditional handicraft is compared and is not separated out, do not precipitate, and sees the contrast test among the embodiment for details.
3, to have technology simple for method for preparing of the present invention, is suitable for large-scale industrial production.
Description of drawings
Fig. 1 is that the product of embodiment 1 preparation is observed photo at the sample behind the 180d that keeps sample under 0 ℃, 25 ℃, 40 ℃ respectively;
Wherein: the left side be the sample of 180d of keeping sample under 0 ℃, intermediary is the sample of 180d of keeping sample under 25 ℃, the right side be the sample of 180d of keeping sample under 40 ℃.
The specific embodiment
Below in conjunction with embodiment the present invention is further specified, but institute of the present invention protection domain is not limited thereto.Polyoxyethylene castor oil EL-35 asks commerce and trade Co., Ltd available from Beijing phoenix gift essence; 2,6-di-tert-butyl-4-methy phenol (BHT) is available from Chemical Reagent Co., Ltd., Sinopharm Group; Other raw materials are commercially available prod commonly used.
Embodiment 1
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 75g, lysine hydrochloride 60g, vitamin A 5g, vitamin D
30.7g, vitamin E 20g, sodium selenite 0.2g, tween 80 250g, propylene glycol 120g, ethyl hydroxybenzoate 0.2g, 2,6-di-tert-butyl-4-methy phenol (BHT) 0.2g, water is settled to 1L.
The above-mentioned method for preparing that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc comprises the steps:
(1) take by weighing zinc gluconate, lysine hydrochloride in proportion in water, 40 ℃ of abundant stirring and dissolving add sodium selenite again, treat solution clarification after, cross 5 μ m filter membranes, subsequent use;
(2) take by weighing in proportion VA oil, VD3 oil, VE is oily pours in the exsiccant retort; Add antiseptic, antioxidant, be stirred to that the dissolving back adds emulsifying agent fully, co-emulsifier stirs to clarification, temperature transfers to 40 ℃; Slowly add step (1) and make chelated zinc solution, water is settled to 1L, promptly gets.
Zinc dissolubility contrast test
, the prepared chelated zinc of patent 02136013.8 prepares nano-emulsion according to the condition of step (2) in this patent embodiment 1 method for preparing use the identical inorganic zinc of zinc content, commercially available chelated zinc (manufacturer: respectively Chengdu Shi Punuo Bioisystech Co., Ltd); Get the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc that the identical embodiment of zinc content 1 makes simultaneously; The product physical behavior of contrast, the result is as shown in table 1:
Table 1
The result shows: these article granule is near hydrone, and is extremely strong with the bonding force of water, can produce high dissolving diffusibility; Can unrestrictedly mix with water.The product of conventional zinc and commercially available chelated zinc uses through drinking-water, is prone to deposition after dissolving, and stop up water fountain, and the present invention is as clear as crystal even after being dissolved in water, and does not have deposition, can not stop up water fountain.
The reserved sample observing test
Get part embodiment 1 prepared nano-emulsion, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence, and is as shown in Figure 1.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 39nm.
The product weight increment test
(1) EXPERIMENTAL DESIGN
The pig of a pig house is divided into three groups; Be respectively matched group, 1 group of test and test 2 groups; Every group of 8 pigs, wherein matched group does not add any vitamin products, tests 1 group of electrolysis multidimensional product (manufacturer: the great Bioisystech Co., Ltd that reaches of Shijiazhuang City) that in drinking-water, adds commercially available sulfur acid zinc; Consumption is tested 2 groups and in every 1000kg drinking-water, is added the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc that 500ml embodiment 1 makes by recommending consumption.Contrast test has been carried out three batches altogether, and is as shown in table 1.
Table 2
(2) experimental animal
Search for food normally the 12-23kg children pig that body weight is consistent after selecting wean for use.Every batch of test pig is divided into three groups by age in days and body weight.On-test,, the group average weight of every batch of pig was consistent, and end is tested when body weight reaches 65-75kg.
The test natural law is 45 days, and accurate recording is respectively organized feed intake, each settlement of term end business accounts honest material amount.The test pig individuality is weighed simultaneously, calculates the feed consumption/weightening finish ratio of each group.
Experimental result
9 groups of observed datas of three batches of tests of arrangement statistics, row are like table 3, table 4 and table 5.Because each batch test chamber symmetry is divided into groups, and each batch of beginning body weight is different, so when significance test of difference, adopt paired t-test method between group in batches.
Table 3
* the P value is measured the result for paired t-test between each batch organized by stages
Conclusion: visible by table 3, every daily feed intake of test pig is tested 2 groups and is tested 1 group and on average reduced by 1.4% between 1.75-1.85kg, has on average reduced by 2% than matched group, and group difference is not remarkable.
Average daily gain is measured the result and is seen table 4.
Table 4
* the P value is measured the result for paired t-test between each batch group
Conclusion: visible by table 4, test 2 groups and be significantly higher than matched group.
According to computing formula:
Feed conversion rate=feed consumption/weightening finish
Result of calculation shows that the nano-emulsion that trace element contains zinc-amino acid chelate demonstrates obvious superiority.Testing 2 groups of average feed conversion rates is 2.74, and testing 1 group of average feed conversion rate is 2.94.
Calculate the improvement amplitude according to following formula:
Through calculating, the improvement amplitude of testing 2 groups (products that the present invention makes) reaches 6.8%, difference highly significant (P=0.004), and the result is as shown in table 5.
Table 5
* the P value is measured the result for paired t-test between each batch group
Conclusion: can find out that through test the compound vitamin nano-emulsion of zinc-amino acid chelate obviously is being better than the commercially available prod aspect daily gain, the feedstuff-meat ratio.
Embodiment 2
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 76g, methionine 55g, vitamin A 6g, vitamin D
30.6g, vitamin E 15g, sodium selenite 0.3g, tween 80 280g, Polyethylene Glycol 100g, ethyl hydroxybenzoate 0.2g, BHT 0.2g, water is settled to 1L.
Method for preparing is with embodiment 1.
The reserved sample observing test
Get part embodiment 2 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above five kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 41nm.
The product weight increment test
Experimental procedure is with embodiment 1, and through detecting, the improvement amplitude reaches 7.1%.
Embodiment 3
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 78g, methionine 68g, vitamin A 10g, vitamin D
30.5g, vitamin E 23g, sodium selenite 0.2g, polyoxyethylene castor oil EL-35 300g, Polyethylene Glycol 150g, ethyl hydroxybenzoate 0.4g, BHT 0.2g, water is settled to 1L.
Method for preparing is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 43nm.
The product weight increment test
Experimental procedure is with embodiment 1, and through detecting, the improvement amplitude reaches 7.4%.
Embodiment 4
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 78g, lysine hydrochloride 75g, vitamin A 5g, vitamin D
30.6g, vitamin E 16g, sodium selenite 0.3g, polyoxyethylene castor oil EL-35 220g, isopropyl alcohol 150g, ethyl hydroxybenzoate 0.5g, BHT 0.3g, water is settled to 1L.
Method for preparing is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 47nm.
The product weight increment test
Experimental procedure is with embodiment 1, and through detecting, the improvement amplitude reaches 7.2%.
Embodiment 5
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 76g, lysine hydrochloride 80g, vitamin A 10g, vitamin D
30.7g, vitamin E 25g, sodium selenite 0.4g, tween 80 300g, isopropyl alcohol 100g, ethyl hydroxybenzoate 0.5g, BHT 0.3g, water is settled to 1L.
Method for preparing is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 49nm.
The product weight increment test
Experimental procedure is with embodiment 1, and through detecting, the improvement amplitude reaches 7.4%.
Embodiment 6
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is following:
Zinc gluconate 80g, lysine hydrochloride 85g, vitamin A 5g, vitamin D
30.4g, vitamin E 15g, sodium selenite 0.2g, tween 80 220g, isopropyl alcohol 100g, ethyl hydroxybenzoate 0.2g, BHT 0.2g, water is settled to 1L.
Method for preparing is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, and every separated 10d sampling is observed.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, phenomenon such as deposition, opalescence.Transmission electron microscope is observed down, and the drop of this nano-emulsion is spherical in shape, and is evenly distributed, and favorable dispersibility uses the particle diameter tester to measure mean diameter and is 46nm.
The product weight increment test
Experimental procedure is with embodiment 1, and through detecting, the improvement amplitude reaches 7.5%.
Claims (10)
1. compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc is characterized in that every liter of component is following:
Zinc gluconate 50～90g, chelating agen 45～90g, vitamin A 4～14g, vitamin D
30.3～0.9g, vitamin e1 5～30g, sodium selenite 0.1～0.5g, emulsifying agent 150～350g, co-emulsifier 80～220g, antiseptic 0.1～2.0g, antioxidant 0.1～2.0g, water is settled to 1L.
2. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that every liter of component is following:
Zinc gluconate 75～80g, chelating agen 60～85g, vitamin A 5～10g, vitamin D
30.4～0.7g, vitamin e1 5～25g, sodium selenite 0.2～0.4g, emulsifying agent 200～300g, co-emulsifier 90～150g, antiseptic 0.2～0.5g, antioxidant 0.2～0.3g, water is settled to 1L.
3. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, described chelating agen is a kind of or combination in lysine hydrochloride, lysine alkali, methionine, glycine, the arginine.
4. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 3 is characterized in that described chelating agen is lysine hydrochloride or methionine; Further preferred lysine hydrochloride.
5. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1; It is characterized in that described emulsifying agent is a tween 80, Arlacel-80; A kind of or combination among polyoxyethylene hydrogenated Oleum Ricini RH-40, the polyoxyethylene castor oil EL-35, preferred tween 80 or polyoxyethylene castor oil EL-35.
6. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, said co-emulsifier is a kind of or combination in isopropyl alcohol, propylene glycol, Polyethylene Glycol, the glycerol, preferred Polyethylene Glycol or isopropyl alcohol.
7. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that described antiseptic is selected from ethyl hydroxybenzoate, methyl hydroxybenzoate or benzoic acid, preferred ethyl hydroxybenzoate.
8. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that described antioxidant is 2,6-di-tert-butyl-4-methy phenol, Butylated hydroxyanisole, sodium thiosulfate; Preferred 2, the 6-di-tert-butyl-4-methy phenol.
9. the said method for preparing that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc of claim 1 is characterized in that, comprises the steps:
(1) take by weighing zinc gluconate, chelating agen in proportion, add in the suitable quantity of water, 20～40 ℃ of stirring and dissolving add sodium selenite again, treat the solution clarification after, through membrane filtration, chelated zinc solution;
(2) weighing vitamin A, vitamin D in proportion
3, vitamin E, add antiseptic, antioxidant, be stirred to that the dissolving back adds emulsifying agent fully, co-emulsifier stirs to clarification, temperature transfers to 20～40 ℃, slowly adds the zinc solution that closes that step (1) makes, water is settled to 1L, promptly gets.
10. method for preparing as claimed in claim 9 is characterized in that, the filter membrane in the said step (2) is 5 μ m filter membranes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110421123 CN102512442B (en) | 2011-12-15 | 2011-12-15 | Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110421123 CN102512442B (en) | 2011-12-15 | 2011-12-15 | Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102512442A true CN102512442A (en) | 2012-06-27 |
| CN102512442B CN102512442B (en) | 2013-03-20 |
Family
ID=46283696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110421123 Expired - Fee Related CN102512442B (en) | 2011-12-15 | 2011-12-15 | Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102512442B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520102A (en) * | 2013-10-14 | 2014-01-22 | 陕西科技大学 | Vitamin D nano-emulsion and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053561A (en) * | 2015-07-29 | 2015-11-18 | 山东迅达康生物科技有限公司 | Nano-level feed additive and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1467199A (en) * | 2002-07-12 | 2004-01-14 | 上海福赐德营养保健品有限责任公司 | Monozinc di-L-lysine |
| CN101982176A (en) * | 2010-10-18 | 2011-03-02 | 齐鲁动物保健品有限公司 | Compound sodium selenite-vitamin E oral nano-emulsion preparation for livestock and preparation method thereof |
-
2011
- 2011-12-15 CN CN 201110421123 patent/CN102512442B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1467199A (en) * | 2002-07-12 | 2004-01-14 | 上海福赐德营养保健品有限责任公司 | Monozinc di-L-lysine |
| CN101982176A (en) * | 2010-10-18 | 2011-03-02 | 齐鲁动物保健品有限公司 | Compound sodium selenite-vitamin E oral nano-emulsion preparation for livestock and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520102A (en) * | 2013-10-14 | 2014-01-22 | 陕西科技大学 | Vitamin D nano-emulsion and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102512442B (en) | 2013-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1224400C (en) | Vitamin compatible micronutrient supplement | |
| EP3874966B1 (en) | Zero-starch no-binder shrimp fry starter compound feed and preparation method therefor | |
| CN102488186B (en) | Edible nano-scale beta-carotene emulsion free from high pressure homogenization and preparation method thereof | |
| AU2011101750A4 (en) | Aqueous formulation of vitamins and preparation method thereof | |
| CN103120107A (en) | Nutrient enrichment selenium-enriched rice and cultivation method thereof | |
| CN102100304B (en) | Fat-soluble vitamin nano microemulsion oral liquid and preparation method thereof | |
| US8518466B2 (en) | Selenium covered basic copper chloride, its preparation method and the use | |
| CN105124603A (en) | Phytosterol-protein compound, as well as preparation and application thereof | |
| CN101434405A (en) | Preparation and use of crystal basic copper chloride containing iron | |
| CN109965148A (en) | Mulberry-leaf extract inhibit freshwater fish culturing stress aspect application and feed and feed producing method | |
| CN102512442B (en) | Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same | |
| CN101530466A (en) | Nano medicament containing oregano oil and vitamins | |
| KR101403511B1 (en) | The method of Eco-friendly alcohol-free, water-soluble propolis | |
| CN102048750A (en) | Amino acid-contained complex multivitamin microemulsion preparation and preparation method thereof | |
| CN101797015A (en) | Preparation method of composite premix feed and obtained composite premix feed | |
| CN106243176B (en) | The copper complex formazan preparation method of sucrose | |
| CN115517267B (en) | Composite iodine solution with high partition coefficient for cultivation and preparation method thereof | |
| CN104719631A (en) | Special vitamin nutrition agent for seawater industrial culture of Atlantic salmon, and preparation method thereof | |
| CN107373144B (en) | Zinc fumarate complex and preparation method and application thereof | |
| CN115777939A (en) | Iron-supplementing oral liquid and preparation method thereof | |
| CN115989873A (en) | Nutritional composition for pregnant and lying-in women and preparation method and application thereof | |
| CN104799083A (en) | Turbot protective agent capable of preventing bean pulp from damaging intestinal mucosas | |
| CN104706636A (en) | Albendazole preparation and preparation method thereof | |
| EP3685676B1 (en) | Zero-starch and binder-free starter compound feed for fish, and preparation method thereof | |
| CN103053807A (en) | Water-soluble composite vitamin preparation and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130320 Termination date: 20141215 |
|
| EXPY | Termination of patent right or utility model |