CN102512368A

CN102512368A - Production technology of metallothionein flexible nano-liposomes

Info

Publication number: CN102512368A
Application number: CN2011104188853A
Authority: CN
Inventors: 汪志友
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-12-15
Filing date: 2011-12-15
Publication date: 2012-06-27

Abstract

The invention discloses a production technology of metallothionein flexible nano-liposomes to people by aiming at the defect that metallothionein and the compound of the metallothionein and other substances (such as common nano-liposomes) are difficult to freely permeate a cell membrane. The production technology comprises the steps of: taking certain amounts of the metallothionein, a protein stabilizer or a freeze-drying protective agent to prepare a buffer solution with a certain concentration; taking certain proportions of phospholipid, chelesterol, surfactant, solvent and the like to prepare an organic phase solution; using a high-pressure pump to fully mix the buffer solution and the organic phase solution for producing a mixed dispersing agent; using an ultrahigh pressure nano homogenizing machine to produce the mixed dispersing agent into nano-liposomes witih more uniform particle sizes; filtering the nano-liposomes through a nano ultrafiltration membrane; and freeze drying and storing. A product which is produced according to the production technology has better storage stability and cell permeability, and can be widely used for making facial cosmetics and preventing and treating cardiocerebral vascular diseases and various diseases.

Description

A kind of production technology of metallothionein flexible nano liposome

Technical field

The present invention relates to a kind of production technology of metallothionein liposome, relate in particular to a kind of production technology of metallothionein flexible nano liposome, belong to field of biological pharmacy.

Background of invention

Metallothionein is one type and is rich in the cysteine group; Various heavy there is high affinity; Under certain PH (pH value) and cellular oxidation-reduction potential environment (but mat organelle somewhere glutathion has reduced form and oxidized form ratio, the demonstration of GSH/GSSG ratio) condition, be prone to and the bonded polypeptide proteins of metal ion such as a plurality of cadmiums, zinc.Mammiferous metallothionein is made up of about 61 aminoacid, contains 20 cysteine and 7 metal ions.Metallothionein has the effective biological functions of multiple uniqueness such as improving body immunity, opposing heavy metal poisoning, storage, transportation and the metabolism of participating in trace element, Plumbum removing zinc supplement, radioprotective, slow down aging, each organ of protection human body cardiopulmonary as a kind of stress protein, free radical resisting oxidant, cell zinc ion mediation agent etc.; Metallothionein is striden the non-memebrane protein macromole of film translation sequence (MTS) and nucleus positioning sequence (NLS) as an acellular film; Use or use or as metallothionein during separately exposed, be difficult to pass freely through cell membrane or nuclear membrane and bring into play its effect with other material complex (like common nanometer liposome) use as the metal ion-chelant metallothionein.Metallothionein is processed the flexible nano liposome, can improve its stability, especially cell membrane permeability: can pass micro channel than the decimal Radix Achyranthis Bidentatae of liposome own.

Summary of the invention

The objective of the invention is; To being the shortcoming that bare metallothionein, metal ion-chelant metallothionein or existing known metal sulfoprotein and other material complex (like common nanometer liposome) all are difficult to pass freely through cell membrane; Biological small duct permeability metallothionein products such as better cell fenestra have been proposed to have, a kind of production technology of metallothionein flexible nano liposome.

Technical scheme of the present invention is achieved in that a kind of production technology of metallothionein flexible nano liposome, realizes that the principal character of the method for this technology comprises the following steps:

1, get metallothionein, protein stabilizing agent or the freeze drying protectant (0.1-5w/v%) in a certain amount of any source, being mixed with concentration is (buffering) aqueous solution of 10-500mM, and the pH value of control solution is between 6.5 to 9.0.

2, get a certain proportion of phospholipid (0.5-10w/v%), cholesterol (0.5-10w/v%), surfactant (0.5-10w/v%), solvent (5-95v/v%) etc. and process organic facies liquid;

3, above buffer and organic phase solution are imported high-pressure pump, fully be mixed and made into mixed dispersion liquid under the condition of nitrogen protection comprising;

4, with above mixed dispersion liquid 4～80 ℃ of homogenizing operating pressure 5-480MPa, homogenizing operating temperatures, comprise under the condition of nitrogen protection and to process nano dispersion fluid through supertension nanometer homogenizer;

5, be the ultrafilter membrane of 100-200nm with above nano dispersion fluid through the aperture, comprise under the nitrogen protection condition and filtering, process metallothionein flexible nano liposome;

6, with above metallothionein flexible nano liposome through lyophilization, bottling, inflated with nitrogen, seal, in 4～20 ℃ of preservations.

Theoretical foundation of the present invention is:

The surfactant molecule of embedding metallothionein flexible nano liposome coating layer can be accumulated at the high pressure position and produce deformation, forms bigger high curvature structure; This structure can reduce the energy dissipation in nanoparticle formation and the storage process; Thereby make the flexible nano liposome have the shape variable property of height; Make it the penetrating aperture micro channel littler 5 times efficiently than itself particle diameter; And common nanometer liposome then can not pass through when its particle diameter is 1.7 times of micro channel fully.The flexible nano liposome has also been given hydrophilic, permeability and the stability of liposome height, thereby has overcome metallothionein owing to lack many obstacles of sending to cyton, organelle, organism of striding that film translation sequence (MTS) and nucleus positioning sequence (NLS) cause.

Effect effect of the present invention is:

1, through using a series of modern nanometer pharmaceutical production technology such as high-pressure pump mixing, the pelletize of supertension nanometer homogenizer, ultrafiltration membrance filter, the quality of strict control nanometer liposome is prepared more even, the controlled nanometer liposome of particle size distribution;

2, through above-mentioned preparation technology, select rational lipid, surfactant, protein stabilization protective agent prescription for use, obtain high performance flexible nano liposome;

3, through nitrogen protection, flexible nano liposome preparation technology, improved the stability of liposome, comprise storage stability;

4, through above-mentioned preparation technology; Prepared nanometer liposome particle size distribution is 30-300nm, be typically 80-120nm; Mean diameter is 80-200nm, be typically 100nm; Encapsulation ratio is 50-99%, is typically 80-90% that the stable storing phase is 4-12 month, is typically 6-8 month;

5, the flexible nano liposome through making makes metallothionein see through micro channel on the little cyton to 10-30nm in aperture, organelle, the organism etc. efficiently.

The specific embodiment

Embodiment one: the purity that 100mg is extracted by Hepar Leporis seu Oryctolagi is 99% metallothionein, 1.5g sodium alginate, 100mg glutathion; Being dissolved in the pH value that 100ml is made into by injection stage water is that 6.8 concentration are in the 100mM phosphate PBS buffer solution; Mix homogeneously; Remove by filter insoluble matter, place 5 ℃ of ice baths or refrigerator; Get 3g lecithin, 100mg cholesterol, 200mg sodium cholate, 200mg DSPC, be dissolved in the mixed solvent of being made up of 25ml water and 75ml dehydrated alcohol, mix homogeneously removes by filter insoluble matter, places 5 ℃ of ice baths or refrigerator.

Above buffer and organic phase solution are imported in the high-pressure pump,, process mixed dispersion liquid fully mixing 5 minutes less than under 15 ℃, the condition of nitrogen protection.

Above mixed dispersion liquid is imported homogenizing operating pressure feeder, open nitrogen protection, under 150MPa15 ℃ of condition,, process nano dispersion fluid through supertension nanometer homogenizer.

Is the ultrafilter membrane of 100nm with above nano dispersion fluid through the aperture, under the nitrogen protection condition, filters, and processes metallothionein flexible nano liposome, and its particle size distribution is that 60-120nm, envelop rate are 91%.

With above metallothionein flexible nano liposome through lyophilization, bottling, inflated with nitrogen, seal, in 4 ℃ of preservations.

Embodiment two: the purity that 150mg is extracted by Hepar Leporis seu Oryctolagi is 99% metallothionein, 2.0g mannitol, 200mg glucosan; Being dissolved in the pH value that 100ml is made into by injection stage water is that 6.8 concentration are in the 100mMTris-HCl buffer solution; Mix homogeneously; Remove by filter insoluble matter, place 5 ℃ of ice baths or refrigerator; Get 5g lecithin, 150mg cholesterol, 250mg sodium cholate, 200mg Polysorbate, 250mg DSPC; Be dissolved in the mixed solvent of forming by 20ml water and 100ml dehydrated alcohol; Mix homogeneously removes by filter insoluble matter, places 5 ℃ of ice baths or refrigerator.

Is the ultrafilter membrane of 100nm with above nano dispersion fluid through the aperture, under the nitrogen protection condition, filters, and processes metallothionein flexible nano liposome, and its particle size distribution is that 75-120nm, envelop rate are 87%.

Claims

1. the production technology of a metallothionein flexible nano liposome, its characteristic comprises the following steps:

A, get a certain amount of metallothionein, protein stabilizing agent or freeze drying protectant, be mixed with certain density buffer solution.

B, get a certain proportion of phospholipid, cholesterol, surfactant, protein stabilizing agent or freeze drying protectant, solvent etc. and process organic phase solution.

C, above buffer and organic phase solution fully are mixed and made into mixed dispersion liquid through high-pressure pump.

D, mixed dispersion liquid is processed the more equally distributed nanometer liposome of particle diameter through supertension nanometer homogenizer.

E, with nanometer liposome through the nanometer ultrafiltration membrance filter.

F, lyophilization, preservation.

2. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

The said metallothionein of steps A comprises natural extract, synthetic, all metallothioneins genetic engineering-dna recombinant expression, people-animal-plant-microorganism source; Said protein stabilizing agent or freeze drying protectant are one or more of sucrose, trehalose, mannitol, glutathion, lactose, glucose, maltose, glucosan, albumin, L-serine, sodium glutamate, alanine, glycine, sarcosine, phosphate, acetate, citrate, taurine, chitosan, oligochitosan etc.; Said concentration is 10-500mM; Said solution comprises deionized water, sterile deionized water, water for injection, PBS or similar solution such as other phosphate buffer, Tris-HCl buffer.

3. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

The said phospholipid of step B is phosphatidylcholine (lecithin; PC), PHOSPHATIDYL ETHANOLAMINE (cephalin; PE), Phosphatidylserine (PS), one or more of phosphatidylinositols (PI), phosphatidyl glycerol (PG), diphosphatidylglycerol phosphoglycerides (containing modified glycerol phospholipid, phosphoglyceride derivant such as DSPC DSPC) such as (cuorins); Said surfactant is that sodium cholate, cholic acid potassium, sodium deoxycholate, deoxycholic acid potassium, fatty acid Pyrusussuriensis are smooth, Polysorbate, sucrose ester, dioctyl sodium sulfosuccinate, sodium laurate etc. one or more; Said solvent be ethanol, water equal solvent etc. one or more.

4. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

The condition of step C is mixing temperature-5-30 ℃, incorporation time 30 seconds to 30 minutes, comprises nitrogen protection.

5. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

The said supertension nanometer of step D homogenizer homogenizing operating pressure is that 5-480MPa, homogenizing operating temperature are 4～80 ℃, comprises nitrogen protection; The system of processing through this step is called nano dispersion fluid.

6. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

The said ultrafilter membrane of step e aperture is 100-200nm, and filter process comprises nitrogen protection, and the system of processing through this step is called the flexible nano liposome.

7. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that:

Steps A-E comprises storage tank, the liquid inventory metering valve of solution, and the automatic control system that is made up of storage tank, valve, high-pressure pump, supertension nanometer homogenizer, production process mass detecting instrument instruments and meters etc.

8. according to the production technology of the said metallothionein flexible nano of claim 1 liposome, it is characterized in that: the metallothionein flexible nano liposome particle size distribution that makes is that 30-400nm, mean diameter are that 80-200nm, encapsulation ratio are 50-99%, stable storing phase to be 4-12 month.