CN102511511B - Nano-antibacterial emulsion containing mint oil and preparation method thereof - Google Patents
Nano-antibacterial emulsion containing mint oil and preparation method thereof Download PDFInfo
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- CN102511511B CN102511511B CN201110407688.1A CN201110407688A CN102511511B CN 102511511 B CN102511511 B CN 102511511B CN 201110407688 A CN201110407688 A CN 201110407688A CN 102511511 B CN102511511 B CN 102511511B
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Abstract
The invention provides a nano-antibacterial emulsion containing mint oil and a preparation method thereof, which belongs to the technical field of embedding of emulsion. A mint oil nanoemulsion system provided in the invention is prepared from the components of mint oil, medium chain triglyceride, an emulsifier, water, etc. Innovation of the invention lies in that a mixture of mint oil and medium chain triglyceride is used as an oil phase for preparation of emulsion, which enables the phenomenon of creaming caused by low density of mint oil to be avoided and the stable nanoemulsion system to be prepared through high pressure homogenization. In antibacterial experiments, the mint oil nanoemulsion provided in the invention has a better antibacterial effect, longer antibacterial time and an effect of controlled release compared to unembedded mint oil. According to the invention, the advantages of a novel design concept, simple flow and strong practicability are realized; and the prepared mint oil nanoemulsion can be used as a safe and long-acting antibacterial agent for food and has a good application prospect.
Description
Technical field
A kind of nano-antibacterial emulsion that contains peppermint oil and preparation method thereof, be specifically related to take modified starch as emulsifier, mixing peppermint oil and median chain triglyceride oil (Glycerin, mixed triester with caprylic acid capric acid) is oil phase, adopts high speed dispersion and high pressure homogenization method to prepare nanoemulsions, belongs to emulsion embedding techniques field.
Background technology
In recent years, along with the development of World Economics, the improving constantly of people life and the level of consumption, people realize more and more stronger for the concern of the aspects such as food security, self health.Meanwhile, alimentary codex is also gradually improved, and people also more and more change to the direction of " green " and " natural " for the concern of food.But, the data that provide according to the World Health Organization, pathogenicity food microorganisms remain the biggest problem that jeopardizes World of Food safety and hygiene and people's life.And traditional be all generally to use chemosynthesis or semisynthetic bactericide for suppressing the method for food microorganisms growth, the residual meeting of these chemical bactericides in food produces harm to human health and environment, and it lures carcinous, teratogenesis and easily causes that the problems such as food chronic poisoning are also for food security has been injected hidden danger.And germ also can develop immunity to drugs to chemical bactericide.Therefore, use the demand of safer natural antibacterial agent to increase gradually, this also matches with the trend that people praise highly wholefood.
The essence oil from plant aromatic substance with special odor that to be a class extract with distillation, squeezing mode from the compositions such as the flower of plant, leaf, root, bark, fruit, seed, resin.As far back as late nineteenth century, just there is scientist to be studied the bactericidal action of essential oil.Nowadays, the sterilization idiocratic of essence oil from plant more gets the attention, and becomes one of important sources of natural, the safe antibacterial agent of food industry demand.
In essence oil from plant, peppermint essential oil is one of spices kind of global trade amount maximum, and every annual requirement is very big.Peppermint oil is had many uses, and can be used for the fields such as medicine, food, cosmetics, spices, tobacco industry.From the ancient times, peppermint oil is just used for the treatment of various diseases in a large number, meanwhile, is also used to the preservation of food and fresh-keeping.Have large quantity research to show, peppermint oil has stronger antibacterium, antimycotic and antiviral effect.Nowadays peppermint is widely cultivated, is easy to obtain.And peppermint oil has no side effect to human body, be one of member of good antiseptics for natural food.
But because peppermint oil is volatile, there is the features such as strong and stimulating taste and water-insoluble, brought inevitable trouble to actual production.For example: in production and storage process, peppermint oil is volatile to be scattered and disappeared; In food and drink system, the water-insoluble of peppermint oil and strong taste have suppressed its addition.In recent years, oil-in-water type nanoemulsions embedding techniques is usually used to solve problems.This technology is to adopt water miscible colloidal substance that insoluble lyophobic dust is carried out to embedding effectively.Therefore, with after nanometer emulsified embedding, insoluble lyophobic dust can not only be water-soluble, and can also avoid its volatilization scatter and disappear, reduce because of environment because of
Element causes unnecessary reaction, reaches the effect of slow release simultaneously.
Nanometer emulsified technology, compared with traditional emulsifying technology, is in preparation process, to adopt higher energy, for example: high-pressure homogeneous, supersonic generator etc.Therefore, nanoemulsions has particle diameter less and more homogeneous compared with conventional emulsion and distributes, and the thermodynamics and kinetics stability of emulsifying product is higher, and the storage stability of the embedding product obtaining is also high.And its nano level molecule, makes it release more even, be easy to control.
Summary of the invention
The technical problem to be solved in the present invention is open a kind of nano-antibacterial emulsion that contains peppermint oil and preparation method thereof, and the peppermint oil emulsion with Nano Particle of a kind of high stability, low volatility is provided, and makes in its natural antibacterial agent that is applied in food.
Technical scheme of the present invention: a kind of nano-antibacterial emulsion that contains peppermint oil, its composition is by percentage to the quality: peppermint oil 1% ~ 20%, median chain triglyceride oil 1% ~ 20%, emulsifier 5% ~ 20%, supplies 100% by deionized water.
The described nano-antibacterial emulsion that contains peppermint oil, take Listeria and staphylococcus aureus in the antibacterial experiment that example is carried out, result shows that peppermint oil nanoemulsions of the present invention shows than the better fungistatic effect of the peppermint oil of not embedding, the antibacterial time is more permanent, plays and controls the effect discharging.
The preparation method of the described nano-antibacterial emulsion that contains peppermint oil, comprises the following steps:
(a) accurately weigh the emulsifier in described ratio, be placed in container, and accurately add the deionized water in described ratio, stir, spend the night, obtain water;
(b) accurately weigh peppermint oil and the median chain triglyceride oil in described ratio, obtain mixing oil phase, join in aqueous emulsifier phase-aqueous solution and mix, and stir;
(c) adopt high speed dispersion and high pressure homogenization method that said mixture is prepared to stable nano-antibacterial emulsion.
In step (c), use high speed dispersion and high pressure homogenization method to prepare nanoemulsions, it is 22000 rpm that its high speed dispersion adopts rotating speed, and the operating time is 1min; High-pressure homogeneous employing pressure is 50 ~ 150 MPa, and homogenization cycles is 1 ~ 20 time.
Beneficial effect of the present invention: by selecting suitable oil phase, mix peppermint oil and median chain triglyceride oil, and use modified starch for emulsifier, by high-pressure homogeneous emulsifying device, prepare the nanoemulsions that contains peppermint oil of the present invention.This system has changed the water-soluble of peppermint oil, increases its dissolubility, can be directly used in the liquid systems such as Food & Drink.Meanwhile, strong volatility and the lability of traditional mint type essence have been avoided by embedding.And compared with traditional emulsion, nanoemulsions, because its particle diameter is little, is uniformly dispersed, in nanometer level, the stability of emulsion increases substantially, and is easy to improve storage stability.
From antibacterial effect, the peppermint oil after nanometer embedding still shows stronger inhibition growth of microorganism
Ability.Compared with the peppermint oil of not embedding, nanometer peppermint fat liquor impels peppermint oil slowly to discharge, and increases thin
Holding the time of staying of lotus oil, the time that shows as inhibition growth of microorganism is longer.Therefore, nanoemulsions system can reach the effect of the long-acting control release of peppermint oil.
Accompanying drawing explanation
The particle diameter distribution map of Fig. 1 antibiotic property peppermint oil nanoemulsions.
Fig. 2 peppermint oil and the impact of peppermint oil nanoemulsions on Listeria growth curve.
Embodiment
Embodiment 1
Get modified starch 12g, be dissolved in 78g deionized water, and stir and spend the night, select pure peppermint oil as oil phase, get peppermint oil 10g, after stirring with starch solution, use high speed dispersion and the high-pressure homogeneous peppermint oil emulsion for preparing.
Embodiment 2
Get modified starch 12g, be dissolved in 78g deionized water, and stir and spend the night, select the mixture of peppermint oil 8g and median chain triglyceride oil 2g as oil phase, after stirring with starch solution, use high speed dispersion and high-pressure homogeneously prepare peppermint oil nanometer emulsion of the present invention.
Embodiment 3
Get modified starch 12g, be dissolved in 78g deionized water, and stir and spend the night, select the mixture of peppermint oil 5g and median chain triglyceride oil 5g as oil phase, after stirring with starch solution, use high speed dispersion and high-pressure homogeneously prepare peppermint oil nanometer emulsion of the present invention.
The peppermint oil emulsion preparing in embodiment 1, embodiment 2, embodiment 3 is carried out to the mensuration of particle size and the coefficient of dispersion.Can find out from testing result, along with the increase of median chain triglyceride oil proportion in oil phase, the average grain diameter of emulsion reduces, and the coefficient of dispersion changes and also reduces.Peppermint oil nanoemulsions in embodiment 2 and embodiment 3, storage stability is good, and through a month room storage, particle size changed in 20nm left and right, all occurs without lamination.Fig. 1 is the particle diameter distribution map that contains peppermint oil nano-antibacterial emulsion of the present invention.
Embodiment 4
There is the peppermint oil nanoemulsions of antibacterial characteristics for the mensuration of antibacterial experiment.By the peppermint that contains 0.5%
Culture fluid is got in 36 hours, the medium culture Listeria of the empty emulsion of oil, peppermint oil nanoemulsions and nanometer within the different time, carries out cell counting.Show from the experimental result of Fig. 2, the peppermint oil after nanometer embedding still shows the ability of stronger inhibition growth of microorganism, and antibacterial effect is more permanent than the antibacterial effect of peppermint oil, has slow releasing function.
Claims (3)
1. contain a nano-antibacterial emulsion for peppermint oil, it is characterized in that its composition is by percentage to the quality: peppermint oil 1% ~ 20%, median chain triglyceride oil 1% ~ 20%, emulsifier 5% ~ 20%, supplies 100% by deionized water;
Described emulsifier is modified starch.
2. the preparation method of the nano-antibacterial emulsion that contains peppermint oil claimed in claim 1, is characterized in that comprising the following steps:
(a) accurately weigh the emulsifier in described ratio, be placed in container, and accurately add the deionized water in described ratio, stir, spend the night, obtain water;
(b) accurately weigh peppermint oil and the median chain triglyceride oil in described ratio, obtain mixing oil phase, join in aqueous emulsifier phase-aqueous solution and mix, and stir;
(c) adopt high speed dispersion and high pressure homogenization method that said mixture is prepared to stable nano-antibacterial emulsion.
3. the preparation method of the nano-antibacterial emulsion that contains peppermint oil according to claim 2, it is characterized in that, in step (c), use high speed dispersion and high pressure homogenization method to prepare nanoemulsions, it is 22000 rpm that its high speed dispersion adopts rotating speed, and the operating time is 1min; High-pressure homogeneous employing pressure is 50 ~ 150 MPa, and homogenization cycles is 1 ~ 20 time.
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| CN104000275B (en) * | 2014-05-16 | 2015-07-22 | 上海应用技术学院 | Spearmint essential oil microemulsion formula used for beverage anticorrosion and preparation method thereof |
| CN105494464A (en) * | 2016-01-15 | 2016-04-20 | 广东净霸科技有限公司 | Azadirachtin broad-spectrum air disinfectant and preparation method thereof |
| CN111011694B (en) * | 2019-12-30 | 2023-03-28 | 江南大学 | Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum |
| CN113068786A (en) * | 2021-04-09 | 2021-07-06 | 华南理工大学 | Essential oil nano emulsion and preparation method and application thereof |
| CN112870162A (en) * | 2021-04-14 | 2021-06-01 | 扬州大学 | Preparation method of peppermint oil nano emulsion |
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| US20070048405A1 (en) * | 2005-07-13 | 2007-03-01 | Dewille Normanella T | Liquid nutritional compositions containing unsaturated fatty acids |
| CN101385469A (en) * | 2007-09-10 | 2009-03-18 | 李景茹 | Technique of agricultural menthence preparation and sterilizing effect thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20070048405A1 (en) * | 2005-07-13 | 2007-03-01 | Dewille Normanella T | Liquid nutritional compositions containing unsaturated fatty acids |
| CN101385469A (en) * | 2007-09-10 | 2009-03-18 | 李景茹 | Technique of agricultural menthence preparation and sterilizing effect thereof |
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| 不同种类胶制备β-胡萝卜素乳状液研究;毛立科 等;《食品研究与开发》;20090831;第30卷(第8期);第16页 * |
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