CN102511399B - Acer mono axillary bud path tissue culture method - Google Patents
Acer mono axillary bud path tissue culture method Download PDFInfo
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- CN102511399B CN102511399B CN 201210009746 CN201210009746A CN102511399B CN 102511399 B CN102511399 B CN 102511399B CN 201210009746 CN201210009746 CN 201210009746 CN 201210009746 A CN201210009746 A CN 201210009746A CN 102511399 B CN102511399 B CN 102511399B
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- 241000219240 Acer pictum subsp. mono Species 0.000 title abstract description 7
- 238000012136 culture method Methods 0.000 title 1
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 5
- 239000012879 subculture medium Substances 0.000 claims abstract description 4
- 241000208140 Acer Species 0.000 claims description 36
- 239000002023 wood Substances 0.000 claims description 29
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 239000011159 matrix material Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000001939 inductive effect Effects 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 9
- 238000013459 approach Methods 0.000 claims description 6
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- 238000010899 nucleation Methods 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 239000002574 poison Substances 0.000 claims description 2
- 231100000614 poison Toxicity 0.000 claims description 2
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims 1
- 230000000249 desinfective effect Effects 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 12
- 241001143500 Aceraceae Species 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- 239000003086 colorant Substances 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000406108 Acer fabri Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 208000034189 Sclerosis Diseases 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
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- 230000001154 acute effect Effects 0.000 description 1
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- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
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- 230000002786 root growth Effects 0.000 description 1
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- 210000003462 vein Anatomy 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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CN 201210009746 CN102511399B (en) | 2012-01-13 | 2012-01-13 | Acer mono axillary bud path tissue culture method |
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CN 201210009746 CN102511399B (en) | 2012-01-13 | 2012-01-13 | Acer mono axillary bud path tissue culture method |
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CN102511399A CN102511399A (en) | 2012-06-27 |
CN102511399B true CN102511399B (en) | 2013-03-20 |
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102715087A (en) * | 2012-06-30 | 2012-10-10 | 吴昌海 | Tissue culture reproduction method for liquidambar styraciflua |
CN103404438B (en) * | 2013-08-06 | 2015-03-25 | 巴中七彩林业科技有限公司 | Acer palmatum seed tissue culture method |
CN103392601B (en) * | 2013-08-09 | 2014-10-22 | 南京金埔园林股份有限公司 | Michelia compressa tissue culture propagation method |
CN109247238B (en) * | 2018-11-30 | 2021-09-24 | 四川七彩林科股份有限公司 | Acer negundo L tissue culture seedling ex-vitro rooting method |
CN109662032A (en) * | 2019-03-05 | 2019-04-23 | 延边大学 | A kind of culture medium and method of acer pseudo-sieboldianum tissue cultures |
CN116724889B (en) * | 2023-05-31 | 2024-06-21 | 东北林业大学 | Method for in-vitro rapid propagation by using axillary buds of Acer mono |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101849506A (en) * | 2010-02-26 | 2010-10-06 | 江苏省农业科学院 | Tissue culture and rapid propagation method of acer palmatum |
CN102138528A (en) * | 2011-03-31 | 2011-08-03 | 南京林江园林有限责任公司 | Method for tissue culture of Acer fabri Hance by axillary buds |
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Address after: Dongshan Street run Qi Road Jiangning District of Nanjing City, Jiangsu Province, No. 70 211100 Patentee after: Nanjing Jinpu Landscape Co., Ltd. Address before: 210019 No. 8, Heng Fei Road, Nanjing economic and Technological Development Zone, Jiangsu, Nanjing Patentee before: Nanjing Jinpu Landscape Co., Ltd. |
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TR01 | Transfer of patent right |
Effective date of registration: 20161223 Address after: Dongshan Street run Qi Road Jiangning District of Nanjing City, Jiangsu Province, No. 70 211100 Patentee after: Jin Pu garden Limited by Share Ltd Address before: Dongshan Street run Qi Road Jiangning District of Nanjing City, Jiangsu Province, No. 70 211100 Patentee before: Nanjing Jinpu Landscape Co., Ltd. |
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Denomination of invention: Acer mono maxim axillary bud path tissue culture method Effective date of registration: 20190701 Granted publication date: 20130320 Pledgee: The International Intellectual Property Trading Center Co. Ltd. Pledgor: Jin Pu garden Limited by Share Ltd Registration number: 2019980000107 |
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Date of cancellation: 20200902 Granted publication date: 20130320 Pledgee: HENGQIN INTERNATIONAL INTELLECTUAL PROPERTY EXCHANGE Co.,Ltd. Pledgor: JINPU LANDSCAPE Co.,Ltd. Registration number: 2019980000107 |
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