CN102499181B - Novel method for breeding Clanis bilineata Walkers - Google Patents
Novel method for breeding Clanis bilineata Walkers Download PDFInfo
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Abstract
The invention discloses a novel method for breeding Clanis bilineata Walkers, comprising the following steps of: (1) selecting larvae; (2) preserving the larvae; (3) warming the larvae; (4) airing and catching one couple of moths; (5) mating and separating the moths; (6) visually inspecting oviposition and carrying out microscopic examination; (7) disinfecting and degumming ovum surfaces; (8) hatching and stocking. The method provided by the invention has the advantages that: 1, the indoor larvae-preserving mortality rate of the Clanis bilineata Walkers is low; 2, the larvae are warmed by raising a temperature through an artificial procedure, the pupation rate of the Clanis bilineata Walkers is high, the eclosion and mating rate is high, the ovum laying amount is great and the hatching rate is high; and 3, the larvae are warmed by batches and Clanis bilineata Walker products are put into the market by batches, so as to be good for the stability of a market price and ensure the income of raisers.
Description
Technical field
The present invention relates to cultured insect technical field, be specifically related to the red breeding method of a kind of beans.
Background technology
Greenish brown hawk moth (Clanis Bilineata Walker) is that Lepidoptera Sphingidae greenish brown hawk moth belongs to insect, is distributed in the provinces such as Hebei, Henan, Shandong, peace are micro-, Jiangsu, Hubei, Sichuan, Shaanxi.Greenish brown hawk moth comprises ovum, larva, pupa, 4 worm states of adult throughout one's life.Beans pellet is that larval phase had for five ages, with the form of mature larva, survived the winter, and was commonly called as the beans pellet of going into hibernation to being commonly called as of killing bean hawkmoth larva.Beans pellet is the primary pest of soybean crops, also can endanger mung bean, cowpea etc.Beans pellet takes food soybean leaves, blade can be eaten into and is incised, and when hairworm is many, beans strain can be eaten into light stalk.A beans pellet can be eaten up 2～3 strain beans leaves.
Because the red nutrient component of beans is abundant, there is the custom of edible beans pellet in the many areas of China.Especially Guanyun County, Jiangsu Province, according to examining the history that has more than 300 year edible beans pellet, has developed hundreds of and take the dish that beans pellet is major ingredient, and the various deep processed products such as can, quick-frozen beans pellet.Meanwhile, Guanyun County has developed into the red distribution centre of beans of national maximum, and purchase is from Hunan, Hubei, Anhui, Henan, Hebei, and even the beans on the ground such as northeast are red, is sold to the counties and cities of periphery.It is reported, the broker that Guanyun County, Lianyungang is engaged in the red marketing of beans now has people more than 400,8000 tons of annual turnovers, and a year sale benefit reaches 1.8 hundred million yuan, makes more than 10,000 women realize seasonal employment.According to the statistics made by the departments concerned, within 2005, the red annual turnover of Guanyun County beans is 8000 tons, and the whole county relies on the red related industry benefit of beans to reach 1.8 hundred million yuan.At the beginning of 2007 red 7 months when just listing price per jin up to 300 yuan, at the bottom of August, the beginning of September peaks the phase, even if now also want one jin of seven, eight yuan.At present, for the gaps in market of fresh beans pellet in spring, the cultivation of the artificial greenhouse of beans pellet begins to take shape, and area reaches more than 1000 mu.What adopt is greenhouse booth breeding, the mode of field planting soybean the cultivation of cover net, and flow process is: on (1) market, choose the beans pellet of going into hibernation and plant as beans are red, imbed breeding in greenhouse by solar heat or bow canopy soil; (2) after next spring, greenish brown hawk moth adult flew out, self-copulation, lay eggs, hatch, 1-2 instar larvae is won together with beans leaf, put the cultivation field that has soybean to prior kind in a suitable place to breed; Or the female moth that catches post-coitum is put into the cultivation Tanaka who is covered with fly net; (4) catch the red list marketing of of the right age beans.The breeding work of this process, except the temperature of booth slightly higher than outdoor temperature, outside effective accumulated temperature arrives in advance, pupate, emergence, mating, lay eggs, hatching etc. is all naturally-occurring, almost there is no manual intervention, therefore exist the problems such as the red kind of beans worm keeping lethality is high, pupation rate is low, emergence mating rate is low, egg laying amount is few, incubation rate is low, disease is serious
Summary of the invention
The invention provides the red breeding method of a kind of beans, by the red breeding overall process of beans is carried out to manual intervention, there is the advantages such as the red kind of beans worm keeping lethality is low, pupation rate is high, emergence mating rate is high, egg laying amount is many, incubation rate is high, disease is few, and convenient centralized management, be conducive to the expansion of the red cultivation scale of beans, be conducive to the raising of the red output of beans and culture benefit.
For achieving the above object, the invention provides the red breeding method of a kind of beans, it is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm;
(2) conservation: the indoor seed-breeding shelf of taking of conservation, the red kind of beans spread thinly in plastic box, above cover sandy soil, then plastic box is put on conservation frame, or directly red kind of beans is spread thinly on conservation frame and cover sandy soil; Maintain room temperature 0-10 ℃, humidity 70%-90%;
(3) warm seeds: in 2-8 month, the red kind of the beans of conservation chamber moved to warm seeds are indoor heats up gradually successively in batches, keep short its of flat temperature pupate and sprout wings after reaching suitable temperature;
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, with fluorescent lamp, lure it to fly on the curtain that gauze or stalk make.Inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber;
(5) mating separation of copulating moth: male and female moth is taken apart gently at copulation chamber post-coitum.Necessary, male moth can, under cooling protection, carry out secondary mating;
(6) the visual inspection microscopy of laying eggs: the female moth visual examination intersegmental membrane after separation of copulating moth, destroy have slag point for being with poison moth.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or puts on the plastic film that surrounding has frame and lay eggs; Female moth after laying eggs is by only planting pin from joint dorsal blood vessel with inspection, adipose tissue in abdomen of picking, after being put in and grinding in 1% sodium hydroxide drop on slide, checks under high-power microscope, as find viral inclusion body particle, the ovum producing together with it is destroyed;
(7) disinfection of egg surface is come unstuck: qualified ovum is put into solution and rubbed rapidly, sloughs the colloid on oophyte surface, pulls 18 ℃ of clear water rinsings twice of rear use out, floats moth hair and the bad egg of floating simultaneously; Then with antiseptic solution sterilization, put into 18 ℃ of clear water rinsings extremely without till medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry;
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in bottom, then two dual-purpose hinges are connected, and make breeding box, sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, temperature 15-30 ℃, relative moisture 75%-90%; The red larva of beans of drawing just hatching of collection with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly puts in a suitable place to breed to leaves of plants, and outside air temperature is lower or when rainy, also can be indoor or booth heatable adobe sleeping platform in put in a suitable place to breed again after artificial feeding's to two molting in age.
Advantage of the present invention: the 1. red indoor conservation lethality of beans is low.2. manual program heats up and carries out warm seeds, and the red pupation rate of beans is high, emergence mating rate is high, egg laying amount is many, incubation rate is high.3. warm seeds in batches, control the red product of beans and go on the market in batches, are conducive to the stable of the market price, guarantee raiser's income.4. by visual inspection, microscopy, three steps of the disinfection of egg surface, substantially got rid of the generation of disease during larva cultivates.5. except Soybean Leaves, select Folium sophorae, kudzu leaf as the red feed cultivating of beans, plant is high, and leaf amount is large.Above measure, can significantly improve the red per mu yield cultivating of beans, is conducive to expand the cultivation scale of beans pellet.
Embodiment
A red breeding method, is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm;
(2) conservation: the indoor seed-breeding shelf of taking of conservation, the red kind of beans spread thinly in plastic box, above cover 10-15cm sandy soil, then plastic box is put on conservation frame; Or directly red kind of beans spread thinly on conservation frame and cover 10-15cm sandy soil.Maintain room temperature 0-10 ℃, humidity 70%-90%;
(3) warm seeds: in 2-8 month, the red kind of the beans of conservation chamber moved to warm seeds are indoor heats up gradually in batches successively, every degree/day rising 1-2 ℃, keeps short its of flat temperature pupate and sprout wings after rising to 15-30 ℃;
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, with black light lamp or ultraviolet light, lure it to fly on the curtain that vertically suspended gauze or stalk make; Inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber;
(5) mating separation of copulating moth: copulation chamber temperature 15-30 ℃, ventilate, dark.After mating 8-20 hour, male and female moth is taken apart gently, necessary, male moth can be at 10 ± 1 ℃ of cooling protections, to carry out secondary mating;
(6) the visual inspection microscopy of laying eggs: the female moth visual examination 2-6 intersegmental membrane after separation of copulating moth, destroy have slag point for being with poison moth; The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or cuts off approximately 2/3 wing and the digitus of 3 pairs of pereiopoda, puts on the plastic film that surrounding has frame and lays eggs; Female moth after laying eggs is by only saving dorsal blood vessel with inspection kind of pin from 2-4, adipose tissue in abdomen of picking, after being put in and grinding in 1% sodium hydroxide drop on slide, checks under high-power microscope, as find viral inclusion body particle, the ovum producing together with it is destroyed;
(7) disinfection of egg surface is come unstuck: qualified ovum puts into the caustic soda of 1%-10% or the sodium carbonate liquor of 0.1%-2.0% is rubbed rapidly 1-10 minute, slough the colloid on oophyte surface, pull the rear 15-30 ℃ of clear water rinsing twice of using out, float moth hair and the bad egg of floating simultaneously; Then the formalin that is 1-10% by concentration sterilization 10-30 minute, puts into 15-30 ℃ of clear water rinsing extremely without till medicine; Put into bamboo basket and dry, after then towel blots, put into shady spot and dry;
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in bottom, then two dual-purpose hinges are connected, and make breeding box, sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, temperature 15-30 ± 1 ℃, relative moisture 75%-90%; The red larva of beans of drawing just hatching of collection with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly puts in a suitable place to breed to Soybean Leaves, Folium sophorae or kudzu leaf; Outside air temperature is lower or when rainy, also can be indoor or booth heatable adobe sleeping platform in put in a suitable place to breed again after artificial feeding's to two molting in age.
Below the situation such as the simple growth cycle of introducing lower greenish brown hawk moth, feature so that those skilled in the art better understands this technical scheme.
Greenish brown hawk moth comprises ovum, larva, pupa, 4 forms of adult throughout one's life.
1. adult: the long 40-45 millimeter of body, health and wing yellowish-brown, many fine hair.Cephalothorax dorsal line crineous, belly respectively saves back side trailing edge brownish black band.Fore wing is long and narrow, and there is larger semicircle crineous in the nearly central authorities of leading edge, and there is reddish brown foxiness base portion top, near yellowish-brown relief angle.
2. ovum: nearly spheroidal, diameter 2-3 millimeter.Light green color during primiparity, gradual change yellow-white, darkens before hatching.
3. larva: body is cylindrical shape, and belly is comprised of 10 joints.Pore has on front 8 joints.4 pairs of abdominal foots, raw on 3-6 joint.On octoon, have a caudal horn, length is because of difference in the length of time.The red larvae underwent 5 instars of beans.The identification in the length of time can nose shape and caudal horn feature carry out.1 age and 5 ages are rounded, and 5 age the head breadth than large 9 times of left and right in 1 age.2,3,4 ages triangular in shape, crown point, but width is different, 3 ages from 4 age caudal horn shape different, the caudal horn in 4 ages is slightly curved.
Specific as follows: in 1 age, be caudal horn pitchy, be about 2 millimeters, be oblique craspedodrome, body is long; In 2 ages, caudal horn is filbert, is about 3 millimeters, becomes tiltedly and keeps straight on; In 3 ages, caudal horn is khaki, is about 4 millimeters, is oblique craspedodrome; In 4 ages, caudal horn pistac, is about 6 millimeters, and base portion is thick, and end is slightly bent downwardly; 5 ages, head is green, health yellow green, the yellow granule of close life, from belly the 1st joint, there are 7 pairs of yellow-white twills health both sides, caudal horn yellow green, is about 6 millimeters, and caudal horn base portion is thick, end is bent downwardly, and 1-5 average body in age length is respectively 1 centimetre, 1.4 centimetres, 2.0 centimetres, 3.6 centimetres, 6.8 centimetres.
4. pupa: the long 40-50 millimeter of body, spindle, bronzing.Before 5-7 podomere valve, respectively there is a band ditch.Cremaster triangle, there are many particle projections on surface, and end is bifurcated not.
The enforcement of the technical program is described with specific embodiment below.
Embodiment mono-:
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.The indoor seed-breeding shelf of taking of conservation, spreads red kind of beans in plastic box thinly, above covers 10cm sandy soil, then plastic box is put on conservation frame, maintains 0 ℃ of room temperature, humidity 70%.February, shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every degree/day rises 1 ℃, keeps short its of flat temperature pupate and sprout wings after rising to 15 ℃.The greenish brown hawk moth wing of sprouting wings lures it to fly on vertically suspended gauze with black light lamp after launching.Inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber.15 ℃ of copulation chamber temperature, ventilate, dark.Mating is taken male and female moth apart after 8 hours gently.Female moth visual examination 2 intersegmental membranes after separation of copulating moth, destroy the band poison moth that has slag point.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs.Female moth after laying eggs by only with inspection kind of pin from 2 joint dorsal blood vessels, adipose tissue in abdomen of picking, after being put in and grinding in 1% sodium hydroxide drop on slide, checks under high-power microscope, destroys with the female moth of viral inclusion body particle and the ovum producing thereof.Qualified ovum is put into 1% soda lye and is rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls 15 ℃ of clear water rinsings twice of rear use out, floats moth hair and the bad egg of floating simultaneously.Then the formalin that is 3% by concentration sterilization 10 minutes, puts into 15 ℃ of clear water rinsings extremely without till medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, 15 ℃ of temperature, relative moisture 75%.The red larva of beans of drawing just hatching of collection with argy wormwood, before at 10 in the morning, puts in a suitable place to breed to Soybean Leaves, continues to take food growth.
Embodiment bis-:
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.The indoor seed-breeding shelf of taking of conservation, spreads red kind of beans thinly on conservation frame and covers 10cm sandy soil.Maintain 5 ℃ of room temperatures, humidity 80%.April, shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every degree/day rises 2 ℃, keeps short its of flat temperature pupate and sprout wings after rising to 25 ℃.After the greenish brown hawk moth wing of sprouting wings launches, with ultraviolet light, lure it to fly on the curtain that vertically suspended stalk makes.Inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber.25 ℃ of copulation chamber temperature, ventilate, dark.Mating is taken male and female moth apart after 10 hours gently.Male moth is at 10 ℃ of cooling protections, and for secondary mating.Female moth visual examination 4 intersegmental membranes after separation of copulating moth, destroy the band poison moth that has slag point.The qualified female moth of visual inspection cuts off approximately 2/3 wing and the digitus of 3 pairs of pereiopoda, puts on the plastic film that surrounding has frame and lays eggs.Female moth after laying eggs by only with inspection kind of pin from 3 joint dorsal blood vessels, adipose tissue in abdomen of picking, after being put in and grinding in 1% sodium hydroxide drop on slide, checks under high-power microscope, destroys with the female moth of viral inclusion body particle and the ovum producing thereof.Qualified ovum is put into 0.5% sodium carbonate liquor and is rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls 25 ℃ of clear water rinsings twice of rear use out, floats moth hair and the bad egg of floating simultaneously.Then the formalin that is 3% by concentration sterilization 25 minutes, puts into 25 ℃ of clear water rinsings extremely without till medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, 25 ℃ of temperature, relative moisture 80%.The red larva of beans of drawing just hatching of collection with cypress branch with after kudzu leaf artificial feeding to two molting in age, after at 4 in afternoon, is directly put in a suitable place to breed to kudzu leaf and continues to take food growth in indoor or booth heatable adobe sleeping platform.
Embodiment tri-
Select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm.The indoor seed-breeding shelf of taking of conservation, spreads red kind of beans in plastic box thinly, above covers 15cm sandy soil, then plastic box is put on conservation frame.Maintain 10 ℃ of room temperatures, humidity 90%.August, shift a collection of beans pellet and plant to warm seeds are indoor and heat up gradually, every degree/day rises 2 ℃, keeps short its of flat temperature pupate and sprout wings after rising to 30 ℃.After the greenish brown hawk moth wing of sprouting wings launches, with black light lamp or ultraviolet light, lure it to fly on the curtain that vertically suspended gauze or stalk make.Inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber.30 ℃ of copulation chamber temperature, ventilate, dark.Mating is taken male and female moth apart after 20 hours gently.Female moth visual examination 6 intersegmental membranes after separation of copulating moth, destroy the band poison moth that has slag point.The qualified female moth of visual inspection is directly put into kraft envelope and lays eggs.Female moth after laying eggs by only with inspection kind of pin from 4 joint dorsal blood vessels, adipose tissue in abdomen of picking, after being put in and grinding in 1% sodium hydroxide drop on slide, checks under high-power microscope, destroys with the female moth of viral inclusion body particle and the ovum producing thereof.Qualified ovum is put into 1% caustic soda or 0.5% sodium carbonate liquor is rubbed rapidly 3 minutes, sloughs the colloid on oophyte surface, pulls 30 ℃ of clear water rinsings twice of rear use out, floats moth hair and the bad egg of floating simultaneously.Then the formalin that is 3% by concentration sterilization 30 minutes, puts into 30 ℃ of clear water rinsings extremely without till medicine.Put into bamboo basket and dry, after then towel blots, put into shady spot and dry.Sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, 30 ℃ of temperature, relative moisture 90%.The red larva of beans of drawing just hatching of collection with withy with after Soybean Leaves artificial feeding to two molting in age, after at 4 in afternoon, is put in a suitable place to breed to Soybean Leaves and continues to take food growth in booth heatable adobe sleeping platform.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited to this, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; can expect easily changing or replacing, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of described claim.
Claims (9)
1. the red breeding method of beans, is characterized in that, comprising:
(1) seed selection: select full, healthy and strong, the seamless beans pellet of going into hibernation as the red kind of beans worm;
(2) conservation: the indoor seed-breeding shelf of taking of conservation, the red kind of beans spread thinly in plastic box, above cover sandy soil, then plastic box is put on conservation frame, or directly red kind of beans spread thinly on conservation frame and cover sandy soil, maintain room temperature 0-10 ℃, humidity 70%-90%;
(3) warm seeds: in 2-8 month, the red kind of the beans of conservation chamber moved to warm seeds are indoor heats up gradually in batches successively, every degree/day rising 1-2 ℃, keeps short its of flat temperature pupate and sprout wings after rising to 15-30 ℃;
(4) dry in the air moth one to one: after the greenish brown hawk moth wing of emergence launches, with fluorescent lamp, lure it to fly on the curtain that gauze or stalk make, inspection per hour once, is grasped the greenish brown hawk moth of mating in mating basket gently, is transferred to copulation chamber;
(5) mating separation of copulating moth: at copulation chamber post-coitum, male and female moth is taken apart gently, necessary, male moth can, under 10 ± 1 ℃ of cooling protections, carry out secondary mating;
(6) the visual inspection microscopy of laying eggs: the female moth visual examination intersegmental membrane after separation of copulating moth, what destruction had slag point is band poison moth, the qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or put on the plastic film that surrounding has frame and lay eggs, female moth after laying eggs is by only planting pin from joint dorsal blood vessel with inspection, adipose tissue in abdomen of picking, after being put in 1% sodium hydroxide drop on slide and grinding, under high-power microscope, check, as find viral inclusion body particle, the ovum producing together with it is destroyed;
(7) disinfection of egg surface is come unstuck: qualified ovum is put into the formaldehyde thimerosal of the caustic soda of 1%-10% or the sodium carbonate liquor of 0.1%-2.0% and 1-10% and rubbed rapidly, slough the colloid on oophyte surface, pull twice of 18 ℃ of clear water rinsing of rear use out, float moth hair and the bad egg of floating simultaneously, then with the formaldehyde thimerosal of the caustic soda of 1%-10% or the sodium carbonate liquor of 0.1%-2.0% or 1-10%, sterilize, put into 18 ℃ of clear water rinsings extremely without till medicine, putting into bamboo basket dries, then, after towel blots, put into shady spot and dry;
(8) hatch and put in a suitable place to breed: 30cm * 20cm wooden frame, gauze is coverd with in bottom, and then two dual-purpose hinges are connected, and make breeding box, sterilize and come unstuck after the red ovum of beans be put in breeding box and hatch, temperature 15-30 ℃, relative moisture 75%-90%, draws the red larva of beans of just hatching of collection with withy, argy wormwood or cypress branch, before at 10 in the morning or after at 4 in afternoon, directly put in a suitable place to breed to leaves of plants, outside air temperature is lower or when rainy, also can be indoor or booth heatable adobe sleeping platform in put in a suitable place to breed again after artificial feeding's to two molting in age.
2. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (2), the red kind of beans is spread thinly in plastic box, above covers 10cm-15cm sandy soil.
3. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (4), fluorescent lamp is black light lamp or ultraviolet light.
4. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (5), copulation chamber condition is temperature 15-30 ℃, ventilate, and dark, mating time is 8-20 hour.
5. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (6), visual inspection be that female moth 2-6 intersegmental membrane has or not slag point.
6. the red breeding method of a kind of beans according to claim 1, it is characterized in that, in described step (6), the qualified female moth of visual inspection is directly put into kraft envelope and lays eggs, or cut off approximately 2/3 wing and the digitus of 3 pairs of pereiopoda, put on the plastic film that surrounding has frame and lay eggs.
7. the red breeding method of a kind of beans according to claim 1, it is characterized in that, in described step (6), the female moth that in the abdomen of picking, fat is organized as after laying eggs is obtained from 2-4 joint dorsal blood vessel with inspection kind of pin, after being put in 1% sodium hydroxide drop on slide and grinding, under high-power microscope, check.
8. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (7), the solution used of sterilizing is 3% formalin sterilization, and the time is 10-30 minute.
9. the red breeding method of a kind of beans according to claim 1, is characterized in that, in described step (8), the leaves of plants that the red growth of beans is fed is Folium sophorae or kudzu leaf.
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