CN102498871A - Method for cuttage propagation of cupressus leighton 'Green' - Google Patents
Method for cuttage propagation of cupressus leighton 'Green' Download PDFInfo
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Abstract
A method for cuttage propagation of cupressus leighton 'Green' belongs to the technical field of plant propagation and is characterized by including steps: firstly, establishing a cutting orchard for female parents of cupressus leighton 'Green' on a proper propagation land; secondly, making peat, vermiculite, pearlite and loess into matrix according to a volume ratio of 5-7:1-3:0.5-1.5:0.5-1.5; thirdly, cutting an autumn shoot of last year to obtain a cutting from a female parent of cupressus leighton 'Green' at the cuttage time, early March; fourthly, dipping a base of the cutting in plant rooting agent Sensheng No.1 at the speed 180-220ppm for two to three seconds; fifthly, performing cuttage on a seedling bed prior to management and maintenance; and sixthly, removing mulch for full-light culture on the fifty-fifth to the sixtieth day after cuttage when rooting rate reaches about 90%. By the method, rooting of cuttings is good, and yield reaches above 90%. The method is simple and fast in propagating.
Description
Technical field
The invention belongs to the plant propagation technical field, be particularly related to the living cypress cuttage breeding method of hybridization speed.
Background technology
The living cypress of hybridization speed
Cupressus leighton' Green ' is subordinate to the Cupressaceae Cupressus, evergreen coniferous tree, and arbor, height of tree 20-25m, hat width of cloth 6-9m is thick with leaves tree-like upright rising, taper shape; Trunk was smooth after bark taupe, bark sliver came off, and sprig and gives birth to scale leaf, and blade tip is tapered, the scale leaf dark green leaf color, and winter is yellowish green, smashs to pieces and distributes fragrance; Cone is circular.
The living cypress of hybridization speed requires not strict to soil types, can grow at acidity, neutrality and alkalescence soil, and is barren-resistant, in deep loose fertile soil growth rapidly, requires draining good, must guard against ponding, undergrowth under high temperature and rainy or dry hot climate, anti-frost; Happiness light, well-grown under the full exposure condition can be done street tree and gardens tree, isolated planting or avenue system.
The livings cypress of hybridization speed originates in the Chinese Himalaya, and its ornamental value is high, and wide accommodation all can be planted in China northeast, North China, southwest and subtropical zone, abroad hedges that are used for more, at home or a brand-new kind.Through inquiry to data of literatures, do not see domestic relevant scientific paper and the patent of the living cypress cottage propagation technology of hybridization speed that have at present, this field still belongs to blank.
Summary of the invention
To the problems referred to above that exist in the prior art, the objective of the invention is to design provides a kind of technical scheme of hybridizing the living cypress cuttage breeding method of speed, and it is simple to operate, reproduction speed is fast, and yield rate reaches more than 90%, has filled up the blank of correlative technology field.
The living cypress cuttage breeding method of described hybridization speed is characterized in that may further comprise the steps:
1) choose suitable breeding spot and set up the livings cypress mother of hybridization speed local area cutting orchard, setting up shading rate is the sunshade net of 60-70%, and the cutting orchard seeding row spacing of planting is 40cm * 40cm, and density is 2400-2600 strain/mu;
2) with volume ratio be the peat of 5-7:1-3:0.5-1.5:0.5-1.5: vermiculite: perlite: yellow soil is puddled evenly as matrix; Install matrix with 72 holes dish; Keep the matrix elasticity consistent, preceding 24 hours of cuttage with the 0.4-0.6% liquor potassic permanganate to the matrix disinfection;
3) cutting time is an early March, and cuttage in 1 year 1 time takes the autumn of last one year to cut fringe slightly from hybridize the living cypress female parent of speed; Being cut into length is the cuttage branch of 7-9cm, keeps the top, and lower cut is level and smooth; Wipe out the needle of fringe bar bottom 2.5-3.5cm, leaf site 4.5-5.5cm is stayed on top;
4) the cuttage branch adopts No. 1 plant of gloomy life to urge root agent 180-220ppm dipping fringe bar base portion 2-3 second, and it is the heteroauxin of 1-3:5-7:1-3 by weight ratio that No. 1 plant of described gloomy life is urged the root agent: methyl: the ABT6 root-inducing powder mixes formation;
5) adopt the seedbed cuttage, cuttage degree of depth 2.5-3.5cm, cuttage finishes; Water immediately permeable, treat that the blade face is dried after, to foliage spray; The U.S. mixed liquor of the carbendazim of 700-900 times of liquid and anthrax good fortune, the weight ratio that carbendazim and anthrax good fortune are beautiful is 4-7:4-7, in time builds shed; Cover sealing all around, entrance management maintenance stage with plastic film;
6) after the cuttage 8-12 days, in time water, make water content of substrate reach 60-70%, relative air humidity is more than 95% in the shed; Watered water 1 time in every later on separated 12-18 days, in summer and autumn, temperature is below 36 ℃ in the control canopy, and mist cooling will be carried out in the outer top of canopy; After the cuttage, before root of hair and the germination, all will shade basically, shading rate reaches 75-90%; After the cuttage 55-60 days, when rooting rate reaches 90% left and right sides, remove film and carry out full exposure and cultivate.
The living cypress cuttage breeding method of described hybridization speed is characterized in that step 2) in: peat: vermiculite: perlite: the volume ratio of yellow soil is 5.5-6.5:1.5-2:0.8-1.2:0.8-1.2.
The living cypress cuttage breeding method of described hybridization speed is characterized in that step 2) in: the dry density 0.25-0.35 of said matrix, total porosity 53-56%, macroporsity 14-16%; Fine pore degree 38-40%, maximum specific retention 1.8-1.9, pH value 5.2-5.6, EC value 0.5-0.6; Full nitrogen 1.9-2.0%, full phosphorus 320-330mg/kg, full potassium 8300-8400mg/kg, calcium 1500-1600mg/kg; Magnesium 1600-1700mg/kg, copper 16-17mg/kg, zinc 50-60mg/kg, boron 180-190mg/kg; Iron 5200-5300mg/kg, manganese 170-180mg/kg, molybdenum 3-4 mg/kg, aluminium 13000-14000mg/kg; Plumbous 14-15mg/kg, sodium 2200-2300 mg/kg, nickel 25-27mg/kg, sulphur 1300-1400mg/kg.
The living cypress cuttage breeding method of described hybridization speed, it is characterized in that in the step 4): it is 190-210ppm that No. 1 plant of gloomy life is urged the concentration of root agent, heteroauxin: methyl: the weight ratio of ABT6 root-inducing powder is 1.5-2.5:5.5-6.5:1.5-2.5.
The living cypress cuttage breeding method of described hybridization speed, it is characterized in that in the step 5): the weight ratio that carbendazim and anthrax good fortune are beautiful is 5-6:5-6.
Described peat, vermiculite, perlite, yellow soil, heteroauxin, methyl, ABT6 root-inducing powder, carbendazim, anthrax good fortune U.S.A all can directly buy from market.
The living cypress cuttage breeding method of above-mentioned hybridization speed, the cuttage branch is taken root all right, and yield rate reaches more than 90%, and this method breeding is simple to operate, and reproduction speed is fast, can adapt to the weather soil environment of China, has good market prospects.
The percentage composition that relates in the present specification unless otherwise indicated all refers to weight percentage.
Embodiment
Combine embodiments of the invention and correlation test at present, the present invention is described further.
Embodiment 1:
1) select subacidity, neutrality and alkalescence soil, monomer booth or conjoined green house are set up in the place that draining is good, subterranean water level is lower, have a good transport service, have abundant water resources; Add shading rate and be 65% sunshade net, set up the female local area cutting orchard of the livings cypress of hybridization speed, the seedbed flattens; The wide 110cm in seedbed; Length is 30m, and the seeding row spacing of planting of cutting orchard is 40cm * 40cm, and density is 2500 strain/mus;
2) with volume ratio be the peat of 6:2:1:1: vermiculite: perlite: yellow soil is puddled evenly as matrix; Install matrix with 72 holes dish; Keep the matrix elasticity consistent; Dish specification in cave be long 54cm, wide 27cm, 5.5cm deeply, preceding 24 hours of cuttage with 0.5% liquor potassic permanganate to the matrix disinfection; The dry density 0.30 of said matrix, total porosity 54.89%, macroporsity 15.25%, fine pore degree 39.64%, maximum specific retention 1.82; PH value 5.43, EC value 0.51, full nitrogen 1.99%, full phosphorus 323.13mg/kg, full potassium 8360.77mg/kg; Calcium 1548.18mg/kg, magnesium 1659.28mg/kg, copper 16.77mg/kg, zinc 54.92mg/kg, boron 180.88mg/kg; Iron 5256.12mg/kg, manganese 170.39mg/kg, molybdenum 3.39 mg/kg, aluminium 13633.50mg/kg; Plumbous 14.23mg/kg, sodium 2266.71 mg/kg, nickel 26.87mg/kg, sulphur 1358.16mg/kg;
3) cutting time is an early March, and cuttage in 1 year 1 time takes the autumn of last one year to cut fringe slightly from hybridize the living cypress female parent of speed; Being cut into length is the cuttage branch of 8cm, keeps the top, and lower cut is level and smooth; Wipe out the needle of fringe bar bottom 3.0cm, leaf site 5.0cm is stayed on top;
4) the cuttage branch adopts No. 1 plant of gloomy life to urge 2 seconds of root agent 200ppm dipping fringe bar base portion, and it is the heteroauxin of 2:6:2 by weight ratio that No. 1 plant of described gloomy life is urged the root agent: methyl: the ABT6 root-inducing powder mixes and constitutes;
5) adopt the seedbed cuttage, cuttage degree of depth 3.0cm, cuttage finishes; Water immediately permeable, treat that the blade face is dried after, to foliage spray; The U.S. mixed liquor of the carbendazim of 800 times of liquid and anthrax good fortune, the weight ratio that carbendazim and anthrax good fortune are beautiful is 5:5, in time builds shed; Cover sealing all around, entrance management maintenance stage with plastic film;
6) after the cuttage 10 days, in time water, make water content of substrate reach 60%, relative air humidity is more than 95% in the shed; Later whenever watered water 1 time at a distance from 15 days, in summer and autumn, temperature is below 36 ℃ in the control canopy, and mist cooling will be carried out in the outer top of canopy; After the cuttage, before root of hair and the germination, all will shade basically, shading rate reaches 75%; After the cuttage 55 days, when rooting rate reaches 90% left and right sides, remove film and carry out full exposure and cultivate.
Cuttage is after 8 months moisture, nutrient, illumination management and sterilization form cave dish seedling.The finished nursery specification: more than the high 10cm, root system is complete, robust plant, no damage by disease and insect, yield rate 90%.
Above-mentioned steps 1) shading rate of sunshade net is 60% or 70% in, and density is 2400 or 2600 strain/mus, and female parent highly remains on 40cm; The dry density 0.25 or 0.35 of matrix step 2), total porosity 53% or 56%, macroporsity 14% or 16%, fine pore degree 38% or 40%; Maximum specific retention 1.8 or 1.9, pH value 5.2 or 5.6, EC value 0.5 or 0.6, full nitrogen 1.9% or 2.0%; Full phosphorus 320 mg/kg or 330mg/kg, full potassium 8300 mg/kg or 8400mg/kg, calcium 1500 mg/kg or 1600mg/kg; Magnesium 1600 mg/kg or 1700mg/kg, copper 16 mg/kg or 17mg/kg, zinc 50 mg/kg or 60mg/kg; Boron 180 mg/kg or 190mg/kg, iron 5200 mg/kg or 5300mg/kg, manganese 170 mg/kg or 180mg/kg; Molybdenum 3 mg/kg or 4 mg/kg, aluminium 13000 mg/kg or 14000mg/kg, plumbous 14 mg/kg or 15mg/kg; Sodium 2200 mg/kg or 2300 mg/kg, nickel 25 mg/kg or 27mg/kg, sulphur 1300 mg/kg or 1400mg/kg; In the step 4) No. 1 plant of gloomy life to urge the concentration of root agent be 180 ppm, 190 ppm, 210 ppm or 220ppm, heteroauxin: methyl: the weight ratio of ABT6 root-inducing powder is 1:7:2 or 3:5:2; The weight ratio that carbendazim and anthrax good fortune are beautiful in the step 5) is 4:6 or 6:4; Step 6) mesostroma water content reaches 65% or 70%, and before germination and the root of hair, shading rate reaches 80%, 85% or 90%; Other also can reach beneficial effect of the present invention with embodiment 1.
Below in conjunction with comparative trial the present invention is described further.
Test 1: from hybridize the living cypress female parent of speed, take the autumn of last one year to cut fringe slightly, being cut into length is the cuttage branch about 8.0cm, keeps the top, and lower cut is level and smooth, wipes out the needle about fringe bar bottom 3.0cm, and top is stayed about leaf site 5.0cm.The cuttage branch adopts four kinds of processing (as shown in table 1), each treatment samples given figure 600 strain.Adopt 72 holes dish to carry out cuttage, the cuttage degree of depth is about 2.0cm, the yield rate of each processing of " Invest, Then Investigate " in 8 months.In this test No. 2 plants of gloomy life to urge the root agent be the indolebutyric acid of 6:2:2 by weight ratio: heteroauxin: the ABT6 root-inducing powder mixes and constitutes; It is the heteroauxin of 2:6:2 by weight ratio that No. 1 plant of described gloomy life is urged the root agent: methyl: the ABT6 root-inducing powder mixes formation.No. 1 plant of gloomy life urges root agent, No. 2 plants of gloomy life to urge the root agent to be buied by Senhe Seed Industry Co., Ltd., Zhejiang Pror..
Table 1 plant urges the root agent to hybridizing the influence of the living cypress cottage propagation yield rate of speed
| Test is handled number | A | B | C | D |
| Plant is urged the root agent | No. 2, gloomy life | No. 2, gloomy life | No. 1, gloomy life | No. 1, gloomy life |
| Concentration/ppm | 200 | 500 | 200 | 500 |
| Yield rate/% | 83.2 | 75.1 | 90.1 | 81.5 |
Can know by table 1, the living cypress of the most suitable cottage propagation hybridization speed urge the root agent to be treated to No. 1 plant of gloomy life urging root agent 200ppm, adopt this processing yield rate can reach 90.0%, result of use is significantly higher than other plant and urges the root agent.No. 1 plant of gloomy life urges the root agent to adopt other concentration of the present invention and prescription, compares test, also can reach similar result of use.
Mesostroma composition of the present invention and quality, urge the concentration of root agent and prescription different, yield rate is all had appreciable impact.
Claims (5)
1. the living cypress cuttage breeding method of hybridization speed is characterized in that may further comprise the steps:
1) choose suitable breeding spot and set up the livings cypress mother of hybridization speed local area cutting orchard, setting up shading rate is the sunshade net of 60-70%, and the cutting orchard seeding row spacing of planting is 40cm * 40cm, and density is 2400-2600 strain/mu;
2) with volume ratio be the peat of 5-7:1-3:0.5-1.5:0.5-1.5: vermiculite: perlite: yellow soil is puddled evenly as matrix; Install matrix with 72 holes dish; Keep the matrix elasticity consistent, preceding 24 hours of cuttage with the 0.4-0.6% liquor potassic permanganate to the matrix disinfection;
3) cutting time is an early March, and cuttage in 1 year 1 time takes the autumn of last one year to cut fringe slightly from hybridize the living cypress female parent of speed; Being cut into length is the cuttage branch of 7-9cm, keeps the top, and lower cut is level and smooth; Wipe out the needle of fringe bar bottom 2.5-3.5cm, leaf site 4.5-5.5cm is stayed on top;
4) the cuttage branch adopts No. 1 plant of gloomy life to urge root agent 180-220ppm dipping fringe bar base portion 2-3 second, and it is the heteroauxin of 1-3:5-7:1-3 by weight ratio that No. 1 plant of described gloomy life is urged the root agent: methyl: the ABT6 root-inducing powder mixes formation;
5) adopt the seedbed cuttage, cuttage degree of depth 2.5-3.5cm, cuttage finishes; Water immediately permeable, treat that the blade face is dried after, to foliage spray; The U.S. mixed liquor of the carbendazim of 700-900 times of liquid and anthrax good fortune, the weight ratio that carbendazim and anthrax good fortune are beautiful is 4-7:4-7, in time builds shed; Cover sealing all around, entrance management maintenance stage with plastic film;
6) after the cuttage 8-12 days, in time water, make water content of substrate reach 60-70%, relative air humidity is more than 95% in the shed; Watered water 1 time in every later on separated 12-18 days, in summer and autumn, temperature is below 36 ℃ in the control canopy, and mist cooling will be carried out in the outer top of canopy; After the cuttage, before root of hair and the germination, all will shade basically, shading rate reaches 75-90%; After the cuttage 55-60 days, when rooting rate reaches 90% left and right sides, remove film and carry out full exposure and cultivate.
2. the living cypress cuttage breeding method of hybridization speed as claimed in claim 1 is characterized in that step 2) in: peat: vermiculite: perlite: the volume ratio of yellow soil is 5.5-6.5:1.5-2:0.8-1.2:0.8-1.2.
3. the living cypress cuttage breeding method of hybridization speed as claimed in claim 1 is characterized in that step 2) in: the dry density 0.25-0.35 of said matrix, total porosity 53-56%, macroporsity 14-16%; Fine pore degree 38-40%, maximum specific retention 1.8-1.9, pH value 5.2-5.6, EC value 0.5-0.6; Full nitrogen 1.9-2.0%, full phosphorus 320-330mg/kg, full potassium 8300-8400mg/kg, calcium 1500-1600mg/kg; Magnesium 1600-1700mg/kg, copper 16-17mg/kg, zinc 50-60mg/kg, boron 180-190mg/kg; Iron 5200-5300mg/kg, manganese 170-180mg/kg, molybdenum 3-4 mg/kg, aluminium 13000-14000mg/kg; Plumbous 14-15mg/kg, sodium 2200-2300 mg/kg, nickel 25-27mg/kg, sulphur 1300-1400mg/kg.
4. the living cypress cuttage breeding method of hybridization speed as claimed in claim 1; It is characterized in that in the step 4): it is 190-210ppm that No. 1 plant of gloomy life is urged the concentration of root agent, heteroauxin: methyl: the weight ratio of ABT6 root-inducing powder is 1.5-2.5:5.5-6.5:1.5-2.5.
5. the living cypress cuttage breeding method of hybridization speed as claimed in claim 1, it is characterized in that in the step 5): the weight ratio that carbendazim and anthrax good fortune are beautiful is 5-6:5-6.
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| CN103283447A (en) * | 2013-05-21 | 2013-09-11 | 江苏圣宏农业科技有限公司 | Full-light-break mist cutting and seedling culturing method for greenhouses |
| CN106105971A (en) * | 2016-06-30 | 2016-11-16 | 浙江森禾种业股份有限公司 | Tree-shaped heather cuttage breeding method |
| CN106171877A (en) * | 2016-06-30 | 2016-12-07 | 浙江森禾种业股份有限公司 | Big Ye Fusejila wood cuttage breeding method |
| CN106171371A (en) * | 2016-07-03 | 2016-12-07 | 浙江森禾种业股份有限公司 | Floral leaf Radix Berberidis Amurensis cuttage breeding method |
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| CN106171877A (en) * | 2016-06-30 | 2016-12-07 | 浙江森禾种业股份有限公司 | Big Ye Fusejila wood cuttage breeding method |
| CN106171371A (en) * | 2016-07-03 | 2016-12-07 | 浙江森禾种业股份有限公司 | Floral leaf Radix Berberidis Amurensis cuttage breeding method |
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