CN102478518A - Tape base coating for particle fluorescence detection - Google Patents
Tape base coating for particle fluorescence detection Download PDFInfo
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- CN102478518A CN102478518A CN2010105608636A CN201010560863A CN102478518A CN 102478518 A CN102478518 A CN 102478518A CN 2010105608636 A CN2010105608636 A CN 2010105608636A CN 201010560863 A CN201010560863 A CN 201010560863A CN 102478518 A CN102478518 A CN 102478518A
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- tape base
- particle
- glycerine
- gelatin
- gelatin solution
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Abstract
The invention discloses a tape base overlay for particle fluorescence detection. The tape base coating comprises gelatin solution, glycerin and chelate dispersant VB. The gelatin solution contains gelatin with a mass ratio of 2-6 %; a usage amount of the glycerin is 25-70 ml; and a usage amount of the chelate dispersant is 2.5-10 ml. The tape base coating can well capture air particles, has low fluorescence detection background noise and long usage time, and can substantially enhance practicality of an apparatus.
Description
Technical field
The present invention relates to a kind of tape base overlay that is applicable to the particle fluoroscopic examination, this overlay is used for trapped particle when being used to detect the particle fluorescent characteristic, the biological example particle.
Background technology
Detection technique of fluorescence usually is used in materialogy, biology and other life sciences, and a key character of fluoroscopic examination and surveying instrument is a sensitivity, and sensitivity is mainly determined by the signal to noise ratio (snr) of instrumental optics imaging system.It is to reduce ground unrest that thereby a kind of increase SNR commonly used improves sensitivity of method, and source of background noise comprises minute surface or the diffuse reflection from the fluorescence exciting laser of sample.
Through using low fluorescent material,, can reduce the autofluorescence of tape base like ultra-thin or dense glass substrate.For example, United States Patent(USP) No. 5,095 has disclosed a kind of plastic slide in 213, makes plastic slide opaque and do not have fluorescence basically through a certain amount of carbon black powders in the plastics.
But because particle can be regarded elastic granule as in the air, this material can not rely on the particle in self absorbed air, makes the difficult real-time online analysis that is used for fluoroscopic examination of this material.
Summary of the invention
Based on the problems referred to above,, the invention provides a kind of tape base overlay that is used for the particle fluoroscopic examination for overcoming the deficiency of prior art.
The objective of the invention is to realize through following technical scheme:
A kind of tape base overlay that is used for the particle fluoroscopic examination, its component close spreading agent VB by gelatin solution, glycerine and huge legendary turtle and form.Said gelatin solution is to be formed by the reaction of gelatin and deionized water, and said gelatin solution and glycerine is at 25 ℃ of-60 ℃ of temperature range internal reactions, and closes spreading agent VB formation tape base overlay through huge legendary turtle.Through can effectively reducing the ground unrest of tape base to the coating of tape base, and the airborne particle that can capture preferably of overlay, thereby judge whether particle is biomone in the air, i.e. the fluorescence of particle.
Preferably, the massfraction of gelatin is 2%-6% in the gelatin solution of gelatin and deionized water formation.
Preferably, the consumption of glycerine is 25ml-70ml.
Preferably, huge legendary turtle to close spreading agent VB consumption be 2.5m1-10ml.
Preferably, gelatin solution and glycerine, huge legendary turtle to close the temperature of reaction of spreading agent be 25 ℃-60 ℃.
It is the capture air particle preferably, and the fluoroscopic examination ground unrest is low, and service time is long, and the instrument usability is greatly improved.
Description of drawings
According to accompanying drawing and embodiment the present invention is done further explain below.
Fig. 1 is tape base according to the invention and overlay excited fluorescence spectrogram;
Fig. 2 is tape base according to the invention and the excited fluorescence spectrogram of capturing particle in the proteinase soybean broth.
Fig. 3 is the excited fluorescence spectrogram of the used formulation glycerine of tape base overlay according to the invention.
Fig. 4 is used for a kind of fluorometric investigation figure of a proteinase soybean broth of biological monitoring appearance for tape base overlay according to the invention.
Fig. 5 is used for a kind of fluorometric investigation figure of lactochrome of biological monitoring appearance for tape base overlay according to the invention.
Fig. 6 is used for the fluorometric investigation figure of a kind of NADH of biological monitoring appearance for tape base overlay according to the invention.
Embodiment
Embodiment one: at first take by weighing a certain amount of solid gelatin and deionized water and put into beaker; And the heated and stirred dissolving, with the aging certain hour of the solution after the dissolving, the gelatin solution massfraction of being prepared is 2%; Because gelatin originally can lose water and become solid-state gradually by liquid state in air; Reduced the ability of capturing, therefore needed to add glycerine and make coating glue in a long period, keep liquid, thereby be beneficial to the long-time on-line monitoring analysis of instrument particle in the air.
Secondly, take by weighing a certain amount of glycerine and react with the gelatin solution for preparing, and the control temperature of reaction; Form tape base according to the invention behind the reaction certain hour and apply glue; Because the glycerine fluorogram has a stronger fluorescence peak near 580nm, therefore for the fluorescence that does not make glycerine has influence on ground unrest, needing the consumption of control glycerine is 25ml; Temperature of reaction is 25 ℃, and adds a certain amount of huge legendary turtle and close spreading agent and form uniform solution.
At last, in gelatin solution and glycerine reaction process, add huge legendary turtle and close spreading agent VB formation tape base overlay, the consumption that huge legendary turtle is closed spreading agent VB is 2.5ml.
Embodiment two: at first take by weighing a certain amount of solid gelatin and deionized water and put into beaker; And the heated and stirred dissolving, with the aging certain hour of the solution after the dissolving, the gelatin solution massfraction of being prepared is 4%; Because gelatin originally can lose water and become solid-state gradually by liquid state in air; Reduced the ability of capturing, therefore needed to add glycerine and make coating glue in a long period, keep liquid, thereby be beneficial to the long-time on-line monitoring analysis of instrument particle in the air.
Secondly, take by weighing a certain amount of glycerine and react with the gelatin solution for preparing, and the control temperature of reaction; Form tape base overlay according to the invention behind the reaction certain hour; Because the glycerine fluorogram has a stronger fluorescence peak near 580nm, therefore for the fluorescence that does not make glycerine has influence on ground unrest, needing the consumption of control glycerine is 50ml; Temperature of reaction is 40 ℃, and adds a certain amount of huge legendary turtle and close spreading agent and form uniform solution.
At last, in gelatin solution and glycerine reaction process, add huge legendary turtle and close spreading agent VB formation tape base overlay, the consumption that huge legendary turtle is closed spreading agent VB is 6ml.
Embodiment three: at first take by weighing a certain amount of solid gelatin and deionized water and put into beaker; And the heated and stirred dissolving, with the aging certain hour of the solution after the dissolving, the gelatin solution massfraction of being prepared is 6%; Because gelatin originally can lose water and become solid-state gradually by liquid state in air; Reduced the ability of capturing, therefore needed to add glycerine and make coating glue in a long period, keep liquid, thereby be beneficial to the long-time on-line monitoring analysis of instrument particle in the air.
Secondly, take by weighing a certain amount of glycerine and react with the gelatin solution for preparing, and the control temperature of reaction; Form tape base overlay according to the invention behind the reaction certain hour; Because the glycerine fluorogram has a stronger fluorescence peak near 580nm, therefore for the fluorescence that does not make glycerine has influence on ground unrest, needing the consumption of control glycerine is 70ml; Temperature of reaction is 60 ℃, and adds a certain amount of huge legendary turtle and close spreading agent and form uniform solution.
At last, in gelatin solution and glycerine reaction process, add huge legendary turtle and close spreading agent VB formation tape base overlay, the consumption that huge legendary turtle is closed spreading agent VB is 10ml.
Person skilled in the art should be familiar with; The above embodiment is used for explaining the object of the invention; And be not with opposing qualification of the present invention; As long as in essential scope of the present invention, variation, modification that the foregoing description is done all will drop in the claim scope of the present invention.
Claims (4)
1. tape base overlay that is used for the particle fluoroscopic examination; Its component is closed spreading agent VB by gelatin solution, glycerine and huge legendary turtle and is formed; It is characterized in that said gelatin solution and glycerine is at 25 ℃ of-60 ℃ of temperature range internal reactions, and close spreading agent VB through huge legendary turtle and form the tape base overlay.
2. a kind of tape base overlay that is used for the particle fluoroscopic examination according to claim 1 is characterized in that, the massfraction of gelatin is 2%-6% in the gelatin solution that gelatin and deionized water form.
3. a kind of tape base overlay that is used for the particle fluoroscopic examination according to claim 1 is characterized in that the consumption of glycerine is 25ml-70ml.
4. a kind of tape base overlay that is used for the particle fluoroscopic examination according to claim 1 is characterized in that it is 2.5ml-10ml that huge legendary turtle is closed spreading agent VB consumption.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105608636A CN102478518A (en) | 2010-11-23 | 2010-11-23 | Tape base coating for particle fluorescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105608636A CN102478518A (en) | 2010-11-23 | 2010-11-23 | Tape base coating for particle fluorescence detection |
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| CN102478518A true CN102478518A (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010105608636A Pending CN102478518A (en) | 2010-11-23 | 2010-11-23 | Tape base coating for particle fluorescence detection |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5701012A (en) * | 1996-03-19 | 1997-12-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
| US7531363B2 (en) * | 2003-12-30 | 2009-05-12 | Honeywell International Inc. | Particle detection using fluorescence |
-
2010
- 2010-11-23 CN CN2010105608636A patent/CN102478518A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5701012A (en) * | 1996-03-19 | 1997-12-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
| US7531363B2 (en) * | 2003-12-30 | 2009-05-12 | Honeywell International Inc. | Particle detection using fluorescence |
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Application publication date: 20120530 |