CN102475193A - Heat stress resistant plant extractive feed additive and preparation method thereof - Google Patents
Heat stress resistant plant extractive feed additive and preparation method thereof Download PDFInfo
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- CN102475193A CN102475193A CN2010106101169A CN201010610116A CN102475193A CN 102475193 A CN102475193 A CN 102475193A CN 2010106101169 A CN2010106101169 A CN 2010106101169A CN 201010610116 A CN201010610116 A CN 201010610116A CN 102475193 A CN102475193 A CN 102475193A
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Abstract
The invention discloses a heat stress resistant plant extractive feed additive which is prepared from the following components in percentage by weight: 25-30% of spinadate seed extractive, 25-30% of Coptis chinensis extractive, 13-18% of Scutellaria baicalensis extractive, 13-18% of pericarpium citri reticulatae and 10-15% of 98% betaine. The feed additive can be used for effectively improving the heat stress resistant capability of livestock, has the advantages of no toxic or side effect and no pesticide residue, and is favorable for the health of animals and people. The product is microchemical. Because of adopting the processing technology of dipping, condensing and spraying to dry, the defects of large dose and unstable effective ingredient content because direct smashing and adding are adopted in the prior art can be solved. According to the heat stress resistant plant extractive feed additive, high temperature stress can be effectively resisted, the antioxidant enzyme activity in the livestock can be improved, animals are accelerated to grow, the disease incidence is lowered, and the feed conversion efficiency is improved.
Description
Technical field:
The present invention relates to a kind of anti-heat stress feed additive, relate in particular to a kind of composite vegetables extractive feed addictive of forming by the various plants extract.
The invention still further relates to the preparation method of this anti-heat stress composite vegetables extractive feed addictive.
Background technology:
Heat stress is meant that environment temperature such as is higher than at the stress reaction due to the comfort zone ceiling temperature in the hot-zone.Therefore, briefly, so-called heat stress is exactly the summation of animal for the nonspecific response reaction of the disadvantageous thermal environment generation of its physiology.Along with developing rapidly of intensification high-density rearing mode, summer high temperature all the more looms large to the influence of aquaculture, is paid close attention to by aquaculture and nutrition worker more and more because of heat stress causes livestock and poultry production performance to descend.The feed addictive of existing anti-heat stress mainly is antibiotic and chemicals additive, also useful plant extracts additives.The shortcoming of antibiotic and chemicals class additive is to use side effect very big for a long time; Can cause that pathogenic microorganism develops immunity to drugs on the one hand; Reduce the prevention effect of Animal diseases: can in meat, egg, milk and environment, produce medicament residue on the other hand, thus the serious threat health.For this reason, world many countries has been made restriction and the regulation of progressively eliminating or forbidding to antibiotic and chemicals Application of Additives.The plant extracts immunologic active material can enhancing body immunity function, improve animal anti-stress, resistance against diseases, improve breeding performonce fo animals, have noresidue, be difficult for producing advantage such as drug resistance, be the focus of animal nutrition research in recent years.Selecting plant extract feed additive to alleviate the influence of thermal environment to livestock and poultry production performance, is a kind of measure preferably.Plant extracts is as feed addictive, be change in modern times, in the intensive culture industry, a new branch of science that grows up along with the development of feed industry; Mostly be exploratory; Its systematic research is scarcely out of swaddling-clothes, promotes, also have certain distance apart from practical application and large tracts of land.
Summary of the invention:
The object of the present invention is to provide a kind of livestock and poultry anti-heat stress ability that improves, have no side effect, pollution-free and plant extract feed additive that production cost is low.
Another object of the present invention is to provide the preparation method of this plant extract feed additive.
Additive formula of the present invention is through consulting related data, select kind surplus in the of 20 to have the plant of anti-heat stress, according to its different processing methods, and the animal feeding result of the test sum up and confirm.Spina date seed in this prescription contains betulin, betulic acid, contains jujube kernel saponin (Jujuboside) in addition, can calm the nerves, nourishes heart, arrest sweating.Confirm to have the central nervous system of inhibition to present calmness, syngignoscism through test.The coptis not only has direct bacteria resistance function and anti diar rhea function, and can transfer the nonspecific immunity function because of containing alkaloids such as jamaicin, coptisine, worenine, has effects such as anti-inflammatory, analgesic, diuresis, calmness; Modern medicine study proves that the root of large-flowered skullcap contains compositions such as baicalin and neobaicalein.Its antimicrobial spectrum is wider, to various bacteria, dermatophyte, Leptospira etc., inhibitory action is arranged all.Even it is, still extremely responsive to the root of large-flowered skullcap to the staphylococcus aureus that antibiotic such as penicillin develop immunity to drugs.The root of large-flowered skullcap is hypotensive in addition, calm, diuresis, cholagogic, releasing smooth muscle spasm, anti-ly stress wait effect.Dried orange peel mainly contains citrene, glycosides displayed etc., is the effect of the short digestion of spleen of being good for the stomach.Dried orange peel also has the merit with hundred medicines, then mends as tonic, then rises with hydrargyrum oxydatum crudum, then fall with falling medicine, because of join benefit arranged, rush down, the power of ascending, descending, therefore, have anti-stress effect.Betaine is that the blackstrap that produces in the garden beet sugaring process extracts.Betaine is participated in metabolism as the animal methyl donor; Have the fat metabolism of promotion, relax multiple effects such as stress, improving a poor appetite, improve meat; Betaine also can replace methionine and choline that tangible growth promotion effect is arranged simultaneously, is a kind of feed addictive of Mobyneb.
The present invention's feed addictive is processed by following component: (consumption is a weight portion)
Wild jujube seeds extract 25-30%
Coptis extract 25-30%
Baical Skullcap root P.E 13-18%
Pericarpium Citri tangerinae micropowder 13-18%
98% betaine 10-15%
Described Wild jujube seeds extract preparation technology is for getting spina date seed impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts;
Described coptis extract preparation technology is for getting coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract;
Described Baical Skullcap root P.E preparation technology is for getting root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts;
Described Pericarpium Citri tangerinae micropowder drying and crushing is to the 80-120 order.
Described betaine purity 98%, commercially available.
The optimum weight of the present invention's feed addictive (part) proportioning is:
Wild jujube seeds extract 28.5%
Coptis extract 28.5%
Baical Skullcap root P.E 15.5%
Pericarpium Citri tangerinae micropowder 15.5%
98% betaine 12%
The preparation method that the present invention's feed adds punishment is:
1, gets spina date seed impurity elimination, chopping processing earlier; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts;
2, get coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract;
3, get root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts;
4, with the Pericarpium Citri tangerinae micropowder drying and crushing to the 80-120 order.
5, described betaine purity 98% is commercially available.
6, with above-mentioned 3 steps obtain raw material Wild jujube seeds extract, coptis extract, Baical Skullcap root P.E, Pericarpium Citri tangerinae micropowder, 98% betaine by weight 25-30: 25-30: 13-18: 13-18: 10-15 mix, packing promptly can be made into finished product.
The present invention's feed addictive adds in the feed by 1.0 ‰ of feed weight, is used for nutrition purposes, especially for feeding animals.
Advantage of the present invention is:
1, to be pure natural plant be processed into through extracting to concentrate this product, do not contain forbidden amphetamine, compares with the antibiotics additive to have to have no side effect, drug residue free, be of value to the healthy advantage of animal and human's class;
2, product reaches traceization, owing to adopt lixiviate, concentrated and spray-dired processing technology, has solved directly to pulverize in the past and has added, the big and unsettled shortcoming of active constituent content of dosage;
3, through improving the body non-specific immune function, strengthen the adaptive capacity of livestock and poultry to heat stress environment;
4, endocrine environment in the control agent keeps eubolism, whets the appetite, and improves weightening finish and efficiency of feed utilization.
For showing effect of the present invention, following test data is provided:
The test of 1 antioxidation activity in vitro
1.1 experimental design
Antioxidation activity in vitro adopts hexichol to measure for bitter taste acyl group free radical (DPPH) method.The aqueous solution that test specimen is made into variable concentrations (5,10,50,100,150,200,250 and 500 μ g/mL) is subsequent use, and with the DPPH solution of ethanol preparation 120umoL/L, it is subsequent use to keep in Dark Place simultaneously.The sample of getting 100 μ L variable concentrations respectively is in 96 porocyte culture plates; Add the DPPH solution that 100 μ L are prepared; Mix; Lucifuge is measured OD value Ai with enzyme-linked immunosorbent assay instrument after leaving standstill 30min under the 517nm wavelength, measure the OD value Aj of 100 μ L sample liquid+100 μ L ethanol and the OD value A0 of 100 μ L DPPH solution+100 μ L water simultaneously, parallel determination 3 times.Be calculated as follows this additive to DPPH inhibiting rate (being free radical scavenging activity): DPPH inhibiting rate=[A0-(Ai-Aj)]/A0 * 100% wherein, the OD value of Ai=100 μ L DPPH solution+100 μ L sample liquid; The OD value of Aj=100 μ L sample liquid+100 μ L ethanol; The OD value of A0=100 μ L DPPH solution+100 μ L ethanol.
1.2 the anti-oxidant result of external DPPH
External to DPPH free radical scavenging activity such as table 1 and shown in Figure 1.This feed addictive reaches 77.5% and 82.9% to the clearance rate of free radical respectively when 100 μ g/mL and the 250 μ g/mL.Description of drawings: Fig. 1 is the broken line graph that anti-heat stress additive and VC influence the DPPH free radical scavenging activity.
Table 1 anti-heat stress additive and VC influence the DPPH free radical scavenging activity
1.3 brief summary
Vitamin C is good anti-oxidant.This test is measured the result through external DPPH method and is shown, the anti-heat stress plant extract feed additive of this patent development has stronger radical scavenging activity, and is suitable with ascorbic effect.
2 anti-heat stress plant extract feed additives are to broiler growth performance, relevant physical and chemical index and anti-oxidant research
2.1 experimental design and animal feeding
120 of the healthy 1 age in days Ai Wei mattress meat chick chickens of experimental selection are divided into 4 groups at random, and establishing 1 group is control group; 2,3,4 groups are added anti-heat stress Chinese herb feed additive 0.5 ‰, 1.0 ‰ and 1.5 ‰ respectively in basal diet.Raise by routine, it is since 15 ages in days that the meat chick is carried out that heat stress handles, and kept pouity dwelling place temperature in (33 ± 2) ℃ up at 15 o'clock in afternoon every day from the morning 9, and whole feeding period is divided into 1～21d and is early stage, and 22～42d is 2 stages of later stage.
Table 1 daily ration is formed and trophic level
Annotate: premix is that every kilogram of complete diet pellet provides: zinc 60mg, iron 95mg, manganese 80mg, copper 10mg, iodine 0.35mg; Selenium 0.3mg, retinol1 0 000IU, cholecalciferol 2 750IU, vitamin E 30IU; Vitamin K 32mg, cobalamin 12 μ g, riboflavin 6mg, niacin 40mg; Pantothenic acid 12mg, pyridoxol 3mg, biotin 0.2mg, Choline Chloride 800mg
2.2 detect index and assay method
2.2.1 growth performance
In the weighing in the 21st day and the 42nd day of test, ramming material is used to calculate average daily gain (ADG), on average daily ingestion amount (ADFI) and feed conversion rate (FCR).
2.2.2 the TAOC in serum and the liver, GSH-Px, SOD and MDA Determination on content
In the 21st day and the 42nd day of test, each repeated to randomly draw 5 chickens, and the heart 5mL that takes a blood sample is loaded in the non-anti-freezing vacuum test tube of 10mL, and 3000r/min is centrifugal, and 10min prepares blood serum sample, and freezing depositing in-20 is ℃ to be measured after the packing.TAC in the serum (TAOC), GSH-Px, SOD and MDA Determination on content all adopt Nanjing to build up the kit that bioengineering is developed.
2.2.3 different preferendum granulocyte/lymphocyte (H/L) value
Adopt mtt assay to measure the 21st day and the 42nd day H/L.
2.3 experimental result and analysis
2.3.1, see table 2 to the broiler growth Effect on Performance.
Table 2 anti-heat stress additive is to the broiler growth Effect on Performance
(unit: g)
Can know by table 2: test 0-21 age in days in early stage, test group all is being superior to control group aspect daily gain, daily ingestion amount and the material anharmonic ratio, but difference does not reach the level of signifiance, and 1.0 ‰ and 1.5 ‰ efficiency of feed utilization is superior to other two groups.Test later stage 22-42 age in days, each is organized daily gain and increases progressively successively, and 1.0 ‰ and 1.5 ‰ addition groups and other two groups reach the significance level of difference.Daily ingestion amount 1.5 ‰ test group are significantly higher than control group.Material anharmonic ratio 1.0 ‰ test group significantly are lower than control group, and are not remarkable with other two groups of differences.To sum up, 1.0 ‰ additive capacity, for promoting growth of meat chicken, it is better to improve food utilization efficiency.
2.3.2, see table 3 to the influence of the anti-oxidant index of serum of broilers.
Table 3 anti-heat stress additive is to the influence of serum of broilers polyphenoils enzymatic activity
Can know by table 3: 21 ages in days and 42 ages in days, the T-AOC of each test group, SOD and GSH-PX are and increase progressively trend, each group of MDA trend that tapers off, 2,3 groups SOD is significantly higher than other two groups.1.0 ‰ and 1.5 ‰ each index levels of addition group are close.The big I representative of TAC (T-AOC) and reflection body antioxidase system and non-enzyme system are to the compensatory capacity of external irritant, and body Radical Metabolism state.SOD is the natural scavenger of ultra-oxygen anion free radical, but the catalysis ultra-oxygen anion free radical generates hydrogen peroxide, the toxic action that can remove the superoxide anion pair cell like this.GSH-Px can protect cell membrane to avoid the peroxidating injury of the unrighted acid on its film of radical pair.MDA produces and is considered to free radical through due to the lipid peroxidation, and free radical can cause that the lipid oxidation of cell membrane and blood plasma lipide albumen increase.MDA content in the tissue is commonly used to represent the height of lipid peroxide contents, and can reflect the variation of interior free yl level indirectly.
2.3.3, see table 4 to the influence of the different preferendum granulocyte/lymphocyte of fryer (H/L) value.
Table 4 anti-heat stress additive is to the influence of the different preferendum granulocyte/lymphocyte of fryer (H/L) value
Can be known by table 4: high temperature has improved the ratio of H/L.The anti-heat stress plant extracts additive that adds various dose all has obvious effects to the rising that suppresses the H/L value, and 1.0 ‰ and 1.5 ‰ addition test group significantly are superior to 0.5 ‰ addition group and control groups.Whether have report to show, be in the index of heat stress state at the diagnosis body, the H/L value is a relatively effectively index.This is illustrated on the basal diet basis, adds the anti-heat stress plant extract feed additive of certain level, can slow down the immune bad reaction that high temperature stress cause.
2.4 brief summary
The anti-heat stress plant extracts additive of this result of the test explanation this patent development can improve meat chicken production performance; Lipid peroxide contents during to inhibition free radical, reduction heat stress; Have certain effect, and can slow down the immune bad reaction that high temperature stress cause.Comprehensive function effect and economic benefit, 1.0 ‰ additive capacities are the righttest addition.
3 anti-heat stress plant extract feed additives are to the influence of mouse growth and heat stress
3.1 test there is not meter and animal feeding
Select 45 of health, the uniform Kunming of body weight small white mouses, be female mouse, be divided into 3 groups at random, every group of 3 repetitions, each repeats 5 mouse.The basal diet of each group is identical, and test group 1 is added this anti-stress feed addictive of 0.5 ‰ and 1 ‰ respectively with test group 2 on control group daily ration basis.Test mice adopts 5 mouse group feedings of each repetition, and free choice feeding is freely drunk water.Preliminary trial period 7d, the formal test phase was 4 weeks.Daily ration is formed and trophic level is seen table 1.
Table 1. daily ration is formed and trophic level (unit: %)
Annotate: 1% premix comprises composite mineral matter additive 57.1%, composite additive of trace elements 5.0%, carrier 37.9%.
3.2 sample collection
Feeding experiment finishes, and stops to feed and drinks water, and is exposed to the sun under the burning sun 4 hours; Weigh, centrifugal 10 minutes of 5000 rev/mins of refrigerated centrifuges are used in the exoculation blood sampling rapidly; Getting upper serum, to place-20 ℃ of refrigerator and cooled to freeze to be measured, dissects to get liver, kidney, heart, spleen and weigh.
3.3 mensuration project and method
3.3.1 mouse body weight
On-test and experimental period are weekly that unit regularly weighs with the cage.
3.3.2 the anti-oxidant index of blood
Measure the anti-oxidant index of blood and comprise TAC (T-AOC), MDA (MDA) and superoxide dismutase (SOD), kit is bought and is built up bio-engineering research institute in Nanjing.
3.3.3 blood physiology, biochemical indicator
3.3.3 blood physiology, biochemical indicator
Blood sugar (GLU) adopts the oxidation enzymatic assays, and kit is available from U.S. Beek-man company; Total serum protein (TP) adopts the biuret colorimetric method for determining, and kit is available from the special biotechnology (Nanjing) of Boeing Co., Ltd; Seralbumin (ALB) adopts the bromocresol green method to measure, and kit is available from the special biotechnology (Nanjing) of Boeing Co., Ltd.
3.3.4 organ index
3.4 result and analysis
3.4.1, see table 2. to the influence of mouse body weight
Table 2. anti-heat stress additive is to the influence of mouse body weight
(unit: g)
Respectively to organize difference not remarkable for the unit mouse goes into to try body weight with the cage.Tested for 1 week, test group 1 and test group 2 be respectively than control group high 2.74% and 6.78%, test group 2 and control group significant difference (p＜0.05); Tested for 2 weeks, test group 1 and test group 2 be respectively than control group high 0.81% and 5.73%, test group 2 and control group significant difference (p＜0.05); 3 weeks of test and 4 weeks of test, the body weight of test group and control group does not all have significant difference.
3.4.2 the anti-oxidant index of blood
Table 3. anti-heat stress additive is to the influence of the anti-oxidant index of mouse blood
Discover that test group all is being higher than control group aspect T-AOC and two anti-oxidant indexs of SOD, the MDA test group is lower than control group, and the effect of 1.0 ‰ additions is superior to 0.5 ‰.
3.4.3 the blood physiology biochemical indicator is seen table 4.
Table 4. anti-heat stress additive is to the influence of mouse blood biochemical indicator
High temperature can influence the homeostasis of livestock and poultry, causes blood biochemical component to change, and blood parameters is research heat stress mechanism and the important reference of taking effective anti-heat stress measure.In the serum albumin and globulin be total serum protein, seralbumin is synthetic by liver, except that the carrier as nutriment, also keeps plasma osmolarity, is again a source of body protein simultaneously, is used for repair tissue and energy is provided.Total serum protein is a synthetic important indicator of antimer internal protein.This test is under 4 hours heat stress situation of Exposure to Sunlight, and each organizes albumin and indifference, and total protein hydraulic test group is significantly higher than control group, explains that this additive has tangible anti-heat stress effect.
Blood sugar concentration is considered to body and regulates a sensitiveest index of metabolism, and relevant high temperature differs to the influence report of animal blood glucose concentration.Blood sugar level descends and to think that body passes through decomposition of glycogen and produces available energy, with enhancing body to high temperature stress resistance cause.Blood sugar level raises and then thinks the responsive nervous excitation of high temperature lower body, the adrenal medella secreting function is strengthened, thereby Adrenaline Concentration is raise, and causes hepatic glycogen and decomposes, so blood sugar level raises.Along with the heat stress time lengthening; Because hypophysis-cortex hormone of aadrenaline (ACTH) is to the adjusting of heat stress physiology course; Can make that glucocorticoid raises significantly in the blood plasma, protein catabolism is accelerated, cause gluconeogenesis to be strengthened; It is constant to keep blood sugar, to resist the pressure that external high temperature causes body.This test is added anti-blood sugar that stress the additive group and significantly is lower than control group, explain under the Exposure to Sunlight situation, causes mouse blood sugar to raise, and owing to the anti-heat stress effect of this additive, test group blood sugar is lower.
3.4.4 organ index is seen table 5.
This additive does not all make significant difference to liver index, kidney index, cardiac index and the index and spleen index of mouse.
Table 5. anti-heat stress additive is to the influence of mouse organ index
The pluck organ is as the specific actor of animal body each item physiological function, and its organ index can be similar to the functional status and the pathology situation of these internal organs of reflection.The explanation of this result of the test, the anti-heat stress feed additive of this patent development does not have influence to the mouse internal organs, can be used as safe additive and uses.
3.5 brief summary
Can find out that through result of the test this additive has the effect that promotes the mouse weightening finish, especially growth effect in early stage is more obvious.Lipid peroxide contents during to inhibition mouse free radical, reduction heat stress has certain effect, and the effect of 1 ‰ additions is superior to 0.5 ‰.Can alleviate the variation of the blood parameters that stress cause by high temperature.And the mouse internal organs are not had influence, can be used as safe additive and use.
4 anti-heat stress plant extract feed additives are to piglet growth performance, relevant physical and chemical index and anti-oxidant research
4.1 experimental design and animal feeding
Tested summer and carry out, test is chosen and is selected 48 of even body weight piglets, is Du * length * Dasanyuan hybridized pig.Be divided into 2 groups at random, every group of 3 repetitions, each repeats 8 pigs, after public female half and half, 7 day preliminary trial period, begins test.Experimental period 50 days.Control group: basal diet, test group are fed and are added the daily ration of 1.0 ‰ anti-heat stress plant extract feed additives, and according to NRC (1998) nutritional need preparation basal diet, specifically composition and trophic level are seen table 1.
Table 1 daily ration is formed and trophic level
Annotate: every kilogram of premix is that complete diet pellet provides: vitamin A 115001U, vitamin D 3200IU, vitamin E 150IU, vitamin K 1.5mg, Cobastab
1250 μ g, nicotinic acid 40mg, pantothenic acid 25mg, riboflavin 6mg, choline 500mg, Fe 100mg, Mn 60mg, Zn 100mg, Se 0.3mg, Cu 100mg, I 3mg, calcium, phosphorus, crude protein are measured value, all the other are calculated value.
4.2 sample collection and mensuration
4.2.1 piglet daily gain, feedstuff-meat ratio, diarrhea rate
Weighing on an empty stomach early morning at test 21d and 50d respectively, is unit statistics feed consumption rate with the hurdle, daily gain (ADG)=weightening finish/test fate; Feedstuff-meat ratio (F/G)=feed consumption/weightening finish; Diarrhea rate=[a diarrhoea number summation/(pig's head number * test fate)] * 100%.
4.2.2 serum anti oxidation index
Employing is bought and is built up the kit measurement serum anti oxidation index that bio-engineering research is produced in Nanjing: TAC (T-AOC), superoxide dismutase (SOD) and MDA (MDA).
4.3 result and analysis
4.3.1 influence to piglet growth performance
Table 2 anti-heat stress additive is to the influence of piglet growth performance
Unit: kg
Visible by table 2, though test 21d in mid-term is not remarkable with off-test phase 50d piglet body weight difference, the test group comparison is according to group difference high 0.69% and 5.86%.Mid-term, daily gain was higher by 2.04% than control group; Omnidistance daily gain test group is higher by 16.67% than control group, significant difference (P＜0.05).Omnidistance material anharmonic ratio test group significantly is lower than control group (P＜0.05).Phase hangs down 3.45%, omnidistance low 3.59% to the test group diarrhea rate in test than control group.
4.3.2 influence to piglet serum anti oxidation index
Table 3 anti-heat stress additive is to the influence of piglet serum anti oxidation index
Can know by table 3: 21d, the T-AOC of test group and SOD all are higher than control group, and difference reaches the level of signifiance (P＜0.05) when 50d.All decline to some extent of MDA content test group in blood when 21d and 50d, but difference is not remarkable.
4.4 brief summary
This additive of result of the test explanation has the promotion piglet growth, improves daily gain, reduces the effect of material anharmonic ratio and diarrhoea incidence.To suppress free radical, reduce stress the time lipid peroxide contents, have better effects.
The specific embodiment:
Embodiment 1:
Press column weight amount proportioning and take by weighing raw material (kilogram):
Wild jujube seeds extract 28.5
Baical Skullcap root P.E 15.5
Pericarpium Citri tangerinae micropowder 15.5
98% betaine 12
The preparation method is following:
1, gets spina date seed impurity elimination, chopping processing earlier; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts;
2, get coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract;
3, get root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts;
4, with the Pericarpium Citri tangerinae micropowder drying and crushing to 80-120.
5, described betaine purity 98% is commercially available.
6, with above-mentioned 3 raw materials that step obtains, the hybrid packed finished product that promptly can be made into.
Embodiment 2:
Press column weight amount proportioning and take by weighing raw material (kilogram):
Wild jujube seeds extract 25
Coptis extract 25
Baical Skullcap root P.E 18
Pericarpium Citri tangerinae micropowder 18
98% betaine 14
The preparation method is following:
1, gets spina date seed impurity elimination, chopping processing earlier; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts;
2, get coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract;
3, get root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts;
4, with the Pericarpium Citri tangerinae micropowder drying and crushing to the 80-120 order.
5, described betaine purity 98% is commercially available.
6, with above-mentioned 3 raw materials that step obtains, the hybrid packed finished product that promptly can be made into.
Embodiment 3:
Press column weight amount proportioning and take by weighing raw material (kilogram):
Wild jujube seeds extract 30
Coptis extract 29
Baical Skullcap root P.E 14
Pericarpium Citri tangerinae micropowder 15
98% betaine 12
The preparation method is following:
1, gets spina date seed impurity elimination, chopping processing earlier; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts;
2, get coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract;
3, get root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts;
4, with the Pericarpium Citri tangerinae micropowder drying and crushing to the 80-120 order.
5, described betaine purity 98% is commercially available.
6, with above-mentioned 3 raw materials that step obtains, the hybrid packed finished product that promptly can be made into.
Claims (5)
1. anti-heat stress plant extract feed additive is characterized in that it is to be processed by following materials of weight proportions:
Wild jujube seeds extract 25-30, coptis extract 25-30, Baical Skullcap root P.E 13-18, Pericarpium Citri tangerinae micropowder 13-18,98% betaine 10-15.
Described Wild jujube seeds extract preparation technology is for getting spina date seed impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts (mass ratio of spina date seed and filler starch is 10: 1);
Described coptis extract preparation technology is for getting coptis impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract quality;
Described Baical Skullcap root P.E preparation technology is for getting root of large-flowered skullcap impurity elimination, chopping processing; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts (mass ratio of the root of large-flowered skullcap and filler starch is 10: 1);
Described Pericarpium Citri tangerinae micropowder drying and crushing is to the 80-120 order;
Described betaine purity 98%, commercially available.
2. feed addictive according to claim 1, wherein the weight proportion of each raw material is:
Wild jujube seeds extract 25-30
Coptis extract 25-30
Baical Skullcap root P.E 13-18
Pericarpium Citri tangerinae micropowder 13-18
98% betaine 10-15
3. the preparation method of claim 1 or 2 described feed addictives is characterized in that:
A, get the spina date seed impurity elimination, chopping is handled; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and spina date seed is processed 10: 1 extracts (mass ratio of spina date seed and filler starch is 10: 1);
B, get coptis impurity elimination, chopping is handled; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, with liquid chromatogram the main action component jamaicin in the coptis is measured, and does filling with starch, makes jamaicin account for 10% of extract quality;
C, get root of large-flowered skullcap impurity elimination, chopping is handled; Use the lixiviate of 70%-80% ethanol then, extraction time 2-4 hour, temperature 70-80 ℃, solvent raw materials quality are than 7-9: 1; Spray-drying concentrates again, makees filler with starch, and the root of large-flowered skullcap is processed 10: 1 extracts (mass ratio of the root of large-flowered skullcap and filler starch is 10: 1);
D, with dried orange peel drying and crushing to 80-120 order micro mist;
E, with above-mentioned 4 steps obtain raw material Wild jujube seeds extract, coptis extract, Baical Skullcap root P.E, Pericarpium Citri tangerinae micropowder add 98% betaine by weight 25-30: 25-30: 13-18: 13-18: 10-15 mix, packing promptly can be made into finished product.
4. the preparation method of the said feed addictive of claim 3, wherein the extracting condition of spina date seed, the coptis and the root of large-flowered skullcap is: lixiviate concentration of alcohol 70%, extraction time 3 hours, 75 ℃ of temperature, solvent raw materials quality were than 8: 1; Dried orange peel drying and crushing to 100 order micro mist; The optimum weight proportioning of Wild jujube seeds extract, coptis extract, Baical Skullcap root P.E, Pericarpium Citri tangerinae micropowder, 98% betaine is 28.5: 28.5: 15.5: 15.5: 12.
5. feed addictive according to claim 1 and 2, its method for using are to add in the feed by 1.0 ‰ of feed weight, are used for nutrition purposes, especially for feeding animals.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743701A (en) * | 2012-07-05 | 2012-10-24 | 河北工程大学 | Pharmaceutical composition for resisting heat stress of broiler chickens |
| CN103169955A (en) * | 2012-06-12 | 2013-06-26 | 北京伟嘉人生物技术有限公司 | Chinese herbal medicine mixture for preventing and treating heat stress of chicken |
| CN103271242A (en) * | 2013-04-28 | 2013-09-04 | 中国科学院东北地理与农业生态研究所 | Anti heat stress fodder additive for min pig |
| CN103931928A (en) * | 2014-03-24 | 2014-07-23 | 安徽宏亮饲料科技有限公司 | Pig feed for high resistance to heat stress |
| CN104757294A (en) * | 2015-03-19 | 2015-07-08 | 广东温氏食品集团股份有限公司 | Heat stress resistant yellow feather broiler feed |
| CN105166418A (en) * | 2015-10-13 | 2015-12-23 | 临沂大学 | Anti-heat stress feed additives for rabbits and preparation method thereof |
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| CN1757305A (en) * | 2004-10-10 | 2006-04-12 | 吉林省农业科学院 | Chinese herbal feed additive and preparation method thereof |
| CN101703154A (en) * | 2009-11-09 | 2010-05-12 | 辽宁医学院 | Dorking heat-resistant stress Chinese medicinal herb feed additive and application method |
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| CN1757305A (en) * | 2004-10-10 | 2006-04-12 | 吉林省农业科学院 | Chinese herbal feed additive and preparation method thereof |
| CN101703154A (en) * | 2009-11-09 | 2010-05-12 | 辽宁医学院 | Dorking heat-resistant stress Chinese medicinal herb feed additive and application method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169955B (en) * | 2012-06-12 | 2015-03-18 | 北京伟嘉人生物技术有限公司 | Chinese herbal medicine mixture for preventing and treating heat stress of chicken |
| CN103169955A (en) * | 2012-06-12 | 2013-06-26 | 北京伟嘉人生物技术有限公司 | Chinese herbal medicine mixture for preventing and treating heat stress of chicken |
| CN102743701B (en) * | 2012-07-05 | 2013-10-23 | 河北工程大学 | Pharmaceutical composition for resisting heat stress of broiler chickens |
| CN102743701A (en) * | 2012-07-05 | 2012-10-24 | 河北工程大学 | Pharmaceutical composition for resisting heat stress of broiler chickens |
| CN103271242B (en) * | 2013-04-28 | 2014-04-16 | 中国科学院东北地理与农业生态研究所 | Anti heat stress fodder additive for min pig |
| CN103271242A (en) * | 2013-04-28 | 2013-09-04 | 中国科学院东北地理与农业生态研究所 | Anti heat stress fodder additive for min pig |
| CN103931928A (en) * | 2014-03-24 | 2014-07-23 | 安徽宏亮饲料科技有限公司 | Pig feed for high resistance to heat stress |
| CN103931928B (en) * | 2014-03-24 | 2015-10-28 | 安徽宏亮饲料科技有限公司 | A kind of pig feed of high anti-heat stress |
| CN104757294A (en) * | 2015-03-19 | 2015-07-08 | 广东温氏食品集团股份有限公司 | Heat stress resistant yellow feather broiler feed |
| CN104757294B (en) * | 2015-03-19 | 2018-06-01 | 广东温氏食品集团股份有限公司 | A kind of yellow-feather broiler fodder of anti-heat stress |
| CN105166418A (en) * | 2015-10-13 | 2015-12-23 | 临沂大学 | Anti-heat stress feed additives for rabbits and preparation method thereof |
| WO2018176093A1 (en) * | 2017-03-28 | 2018-10-04 | Iriccorgpharm Pty Ltd | Berbine alkaloid formulations in the prevention and/or treatment of infectious disease |
| CN110709080A (en) * | 2017-03-28 | 2020-01-17 | Irp健康股份有限公司 | Use of berberine alkaloid preparation in preventing and/or treating infectious diseases |
| CN109007349A (en) * | 2018-06-07 | 2018-12-18 | 吉林省农业科学院 | A kind of brood sow anti-oxidation stress feed addictive |
| CN113056204A (en) * | 2018-08-23 | 2021-06-29 | 地绿康生物技术有限公司 | Livestock feed additive |
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