Micro photometer
Technical field
The present invention relates to field of measuring technique, particularly a kind of micro photometer.
Background technology
Ultraviolet-visible spectrophotometer is a kind of light transmission sample that makes, analyze its absorption spectrum and the instrument of or quantitative test qualitative to composition.Generally formed by light source, monochromator, sample cell and detecting device.
Traditional ultraviolet-visible spectrophotometer product, the 10mm light path liquid sample pool of Application standard is more than the testing liquid consumption is generally 2mL.When being used for life science, when particularly measuring albumen, DNA and rna content, sample is generally precious, and relative concentration is higher, thereby must first dilute when using the 10mm sample cell.Dilution consumes solvent, complex operation, and also after dilution, sample can't be recycled.The spectrophotometer for the submicrosample pond of this application occurs in recent years, can directly carry out photometric analysis to the micro updating sample.
The luminosity pond of most of micro photometer, generally be made of two glass or quartzy planes with certain interval (general 0.2mm, 0.5mm or 1mm) on the market.During measurement, two planes separately drop in ad-hoc location (hydrophobic surface commonly used or miniature concave surface fix liquid) on one of them plane with micro-drop, the plane of then closing, and drop is formed thin layer or the miniature cylinder with specific thicknesses by two planes extruding.Two optical fiber of mutually aiming at are installed after two glass planars, and source light passes glass from an optical fiber outgoing, then passes liquid lamella, then passes glass planar, enters detection fiber.The detection fiber other end is connected to monochromator and photoelectric commutator, to analyze the absorption spectrum of liquid.
Some the design in a glass planar can replace with minute surface, and will launch with detection fiber be arranged on homonymy, light is through mirror-reflection, secondary passes glass and liquid, enters reception optical fiber.Because light passes liquid twice, equivalent light path doubles, and can make mechanism's actual gap thinner, and consumable liquid still less.
In said method, all need a machinery folding mechanism, closed later plane distance is the measurement light path, general mechanical register pin or the locating surface of relying on guarantees this distance, from open to the action of closing, if mechanical register pin or locating surface wear and tear, mix dust or liquid, can affect bearing accuracy, and then affect light path, cause measuring error.
Fluid drips on glass or quartzy face, after measurement is completed, needs by hand drop is wiped on the other hand, and this can bring series of problems: the manual wipping slow motion, can't realize high flux and automatic measurement; People's manual manipulation is wrong poor, easily wipes unonly, and particularly the protide sample has stronger adhesiveness, easily remains in glass surface, affects absorbance measuring, and pollutes the sample that next time splashes into; Long-term wipe surfaces, surface abrasion becomes flower, and not only optical property worsens, and hydrophobicity and anti-adhesive all can descend, and need periodic maintenance otherwise affect instrument performance.
Summary of the invention
It is high and realize high flux, robotization and micro photometer that the micro liquid absorbance of exempting to clean is measured that technical matters to be solved by this invention is to provide a kind of measuring accuracy.
For solving the problems of the technologies described above, the invention provides a kind of micro photometer and comprise:
Trace luminosity pond, front supporting member, rear support part, front rolling wheel, back winding wheel, point sample mechanism; The plastic film tape that is wrapped on described front rolling wheel is connected with described back winding wheel by described front supporting member, described point sample mechanism, described micro-luminosity pond, described rear support part successively.
Further, described micro-luminosity pond comprises body, limited block and optical fiber; Have slit on described body, described body is connected with described optical fiber; Be provided with described limited block on described body.
Further, described slit two ends are with the cambered surface that broadens gradually.
Further, the width of the described slit plastic film thickness that equals twice adds the measurement light path.
Further, described micro-luminosity pond also comprises detecting device, and described detecting device analyzes according to the absorbance of described optical fiber the composition and the concentration that drop in drop on described plastic film tape, realizes photometric measurement.
Further, described optical fiber comprises emergent light fibre and incident optical; Described outgoing optical fiber and incident optical divide and are located at described slit two ends.
Further, micro photometer also comprises recover, is arranged on described rear support part place, reclaims the drop on described plastic film tape.
Further, micro photometer also comprises the alveolar fluid clearance device, is arranged on described rear support part place, removes the drop on described plastic film tape.
Further, the described front supporting member of micro photometer and rear support part are roller or pole.
Further, described point sample mechanism is manual liquid-transfering gun, the pipettor pipettor on hyperchannel liquid-transfering gun or mechanical arm.
Micro photometer provided by the invention can be realized the high flux screening of sample can automatically moving round the clock Maintenance free, only needs the regular replenishment film strip.
Description of drawings
The structural representation of the micro photometer that Fig. 1 provides for the embodiment of the present invention.
Fig. 2 is the structural representation in micro-luminosity pond in micro photometer structure shown in Figure 1.
Fig. 3 is the cut-open view in micro-luminosity shown in Figure 2 pond.
Embodiment
Referring to Fig. 1, a kind of micro photometer that the embodiment of the present invention provides comprises micro-luminosity pond 1, front supporting member 2, rear support part 3, plastic film tape 4, front rolling wheel 5, back winding wheel 6 and point sample mechanism 7.The plastic film tape 4 that is wrapped on front rolling wheel 5 is connected with back winding wheel 6 by front supporting member 2, point sample mechanism 7, micro-luminosity pond 1, rear support part 3 successively.Plastic film tape 4 should be optical clear, and has the flexible distortion of certain elasticity, and the plastic film tape that some kind plastic warp extrusion modling is made can satisfy this requirement.Front supporting member 2 and rear support part 3 are roller or pole.Point sample mechanism 7 can be manual liquid-transfering gun, the hyperchannel liquid-transfering gun, and the pipettors on mechanical arm etc. are used for splashing into drop to plastic film tape.For example, point sample mechanism 7 can be 4 passage liquid-transfering guns or 12 passage liquid-transfering guns.The present invention is wound in plastic film tape 4 rolling on front rolling wheel 5, then by front supporting member 2, micro-luminosity pond 1 and rear support part 3, finally is fixed on back winding wheel 6 places, and back winding wheel 6 rotarily drives film by motor-driven and moves, and is similar to the motion of tape.
Shown in Figure 2, micro-luminosity pond 1 comprises body 11, limited block 13 and optical fiber 15.Have slit 14 on body 11.Body 11 is connected with optical fiber 15.Be provided with limited block 13 on body 11.Slit 14 two ends are with the cambered surface that broadens gradually, when plastic film tape 4 enters micro-luminosity pond, are subject to the cambered surface effect, will be folded in half.Owing to there being certain tensile force on film, this tensile force at the slit place along the AB direction laterally, be subject to this masterpiece plastic film tape 4 of exerting oneself and will be close on slit 14 walls, slit 14 width are greater than the plastic foil thickness of twice, so a gap also will be left in plastic foil inside.When limited block 13 is used for keeping doubling, film keeps center line symmetrical, avoids the low folding or rotation of the high side of a side.
Shown in Figure 3, optical fiber 15 comprises outgoing optical fiber 151 and incident optical 152.Outgoing optical fiber 151 and incident optical were located at slit 14 two ends in 152 minutes.Outgoing optical fiber 151 is used for introducing light source.Incident optical 152 is used for test-accepting light.When drop 8 passes through slit 14, formed oblate column.Source light passes film and drop 8 from the 151 end face irradiations of outgoing optical fiber, enters incident optical 152, causes detecting device.In drop, composition to be measured has absorbed the part transmitted ray, according to langbobier law:
Wherein, A is absorbance, I
0Be incident intensity, I is transmitted light intensity, and K is absorptivity, and C is solution concentration, and b is light path, equals slit width in the design and deducts 2 times of film thicknesses.The detecting device (not shown) analyzes according to absorbance the composition and the concentration that drop in drop on plastic film tape, realizes photometric measurement.
The below describes in detail to the principle of work of micro photometer:
Micro photometer when work, Fig. 1 point sample mechanism 7 at first with zero point several sample drops to several microlitres in film central authorities.This moment, drop was with spherical in shape due to the hydrophobic effect on plastic foil surface.Back winding wheel 6 rotates, and drives plastic film tape 4 and travels forward, and to the cambered surface place of slit, by doubling gradually, the distortion that is squeezed of the drop at this place can form oblate column to slit 14 stenosis, the fibre-optical probe that passes through, as shown in Figure 3.Droplet size should be through calculating, and makes body diameter after compression greater than optical fiber outgoing end face height, again less than the plastic tape height, can transmission fluid to guarantee whole light, and drop can not overflow plastic tape and pollute micro-luminosity pond simultaneously.Source light by the coupling fiber transmission fluid, enters detecting device at slit 14 places at last.After measurement was completed, travelling belt continued motion, liquid was sent the stenosis of slit 14, and plastic tape launches with cambered surface, and liquid becomes drop again due to hydrophobic effect, came the rear roller place.Can use this moment liquid-transfering gun to reclaim, perhaps directly abandon.
In whole process, drop does not contact plastic tape parts in addition, thereby mechanism need not wiping.During dropping liquid, need to control certain sample interval, sample room can be in contact with one another and pollute.When using the multichannel pipettor of Biochemical Lab's 12 passages commonly used, this pipettor is disposable 12 duplicate samples that splash at regular intervals, system is according to the corner of this Separation control rolling wheel motor, sample passes through fibre-optical probe successively, and and the detecting device timing synchronization, order is carried out the absorbance of 12 samples and is measured, and can realize that high flux detects.Mechanism also can coordinate robotic arm, consists of automatic testing station, is specially adapted to the Bioexperiment such as genescreen.When liquid need to reclaim, available pipettor was in rear roller place's collection.Reclaim as not needing, can add at the rear roller place sponge and directly wipe sample.
In a typical design, film bandwidth 5mm made of plastic, thickness 0.1mm, slit width 1mm.Optical fiber core diameter 0.4mm, drop is at the thick cylinder of the probe formation 1mm diameter 0.8mm of place, and required sample volume is 0.6uL.The sample room minimum spacing is calculated with 4mm, and a volume film strip is 100 meters, enough measures 25000 samples.Calculating take plastic density as about 2000Kg/m3, only consume approximately 100g of plastics, is feasible economically.System's fully automatic operation under computer is controlled, each sample only need the approximately 2 second time, thereby can realize the per hour high flux screening of the highest 1800 samples, and can move round the clock Maintenance free, only need the regular replenishment film strip.Most of biological experiment uses the UV-VIS spectrophotometry test sample, thereby needs film strip transparent to 220nm to 900nm wave spectrum, does not react with common solvent.Fluorinated ethylene propylene copolymer (FEP) is a kind of chemically inert soft plastic, and good corrosion resistance is arranged; On optics, transparency is excellent, and refractive index is low, and stronger Radiation hardness is arranged, and at visible region, transparency is preferably arranged, and at the dark purple outskirt of 220-300nm, good transmissivity is arranged also, thereby is the preferred material of making film strip of the present invention.
Polystyrene (PS) and polymethylmethacrylate (PMMA) also have transparency preferably to visible light, but the transmissivity below the 270nm wavelength is lower, is applicable to the visible light analysis.FEP is low for this bi-material cost ratio, also can be used for making film strip.Above-mentioned material can be made band shape with extrusion molding or drawing process.
When making the slit in micro-pond and cambered surface, if use metal material, can adopt CNC milling machine, cambered surface should polish smooth.Also can processing mold, cast this parts with plastics or aluminium.After moulding, make the optical fiber micropore with laser, after fiber cores is passed micropore, use adhesive curing, then polish the inboard fiber end face of slit until reach the optical surface requirement with a thin mill.Another kind of job operation is amalgamation again after the vertical dimidiation in micro-pond is processed respectively, the difficulty that can avoid processing slit.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to example, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.