CN102426247A - Preparation and application of universal electrochemical immunosensor based on cotton threads - Google Patents
Preparation and application of universal electrochemical immunosensor based on cotton threads Download PDFInfo
- Publication number
- CN102426247A CN102426247A CN2011102794844A CN201110279484A CN102426247A CN 102426247 A CN102426247 A CN 102426247A CN 2011102794844 A CN2011102794844 A CN 2011102794844A CN 201110279484 A CN201110279484 A CN 201110279484A CN 102426247 A CN102426247 A CN 102426247A
- Authority
- CN
- China
- Prior art keywords
- hsa
- preparation
- cotton thread
- antibody
- curve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention provides preparation and application of a universal electrochemical immunosensor based on cotton threads, belonging to the technical field of electrochemical immunodetection. According to the invention, the nanometer functional material carbon nanotubes are used to wrap traditional cotton threads by using the technology of Dip-Dry so as to obtain high conductivity, and an human serum albumin antibody is added into a wrapping solution so as to prepare a sensor which can specifically detect albumin; according to results of quantitative determination, the prepared sensor can specifically detect albumin in human serum and has detection sensitivity of 119 nM, satisfying the requirement for sensitivity in daily monitoring of human serum. A detection sensor directed at a replacing antibody can be conveniently fabricated by replacing the human serum albumin antibody with the replacing antibody in the preparation of the sensor, and a detection method is also a universal detection method. Compared to conventional detection methods, a universal preparation and detection method for cotton thread sensors provided in the invention is fast, convenient and highly sensitive and can be used in rapid and sensitive detection of proteins in serum.
Description
Technical field
What the present invention relates to is a kind of preparation and application of the universal electrochemical immunosensor based on cotton thread, has also related to the versatility that this detection method detects haemocyanin simultaneously.Belong to electro-chemistry immunity detection technique field.
Background technology
Therefore nano material has many new physicochemical characteristicss because it is small-sized, and structure is special, like small-size effect, and big specific surface, high reactivity, quantum effect etc.Based on the self characteristics of nano material, it is fast that foundation has detection speed accordingly, and the research of highly sensitive detection method becomes research emphasis day by day.CNT because its its specific structure characteristics and electric conductivity receive people's great attention always, had also once gone out not poor based on the development of the sensor of CNT since 1991 are found by Japanese scientist Ijima.But because preparation cost is high, reasons such as method of application difficulty height, bio-compatibility is poor, has limited the actual of sensor of research and development and has applied.In order to overcome above-mentioned shortcoming; The electrochemical immunosensor that research and development economy is easy to apply based on cotton thread, the present invention is a material with cotton thread and textile cloth first, utilizes CNT that cotton thread is carried out the dip-dry parcel; The antibody that in the process of parcel, adds human serum albumin HSA; Preparation can be monitored the fast high-sensitive sensor of human serum albumins, and the present invention has simultaneously also prepared the universal electrochemical immunosensor based on cotton thread, can monitor various haemocyanins.The sensor of particularly setting up is based on the cotton thread that can be woven into corresponding textile garment, for simple, convenient in the daily life later on and monitoring whenever and wherever possible provide possibility.
Summary of the invention
The objective of the invention is to prepare a kind of can be quick, detect albuminous cotton thread immunosensor in the serum with sensitivity.
Two of the object of the invention be set up based on the human serum albumins sensor of preparation fast, high-sensitive detection method and technical indicator.
Three of the object of the invention is on the basis of goal of the invention one, two, sets up the preparation and the detection technique of universal cotton thread immunosensor.
Technical scheme of the present invention:
1. the preparation method of the galvanochemistry cotton thread sensor of monitor's seralbumin (HSA), preparation process is:
(1) preparation of CNT kayexalate solution: kayexalate PSS is scattered in obtains the PSS solution that mass concentration is 5%-15% in the WS; Water-soluble carbon nanometer tube is scattered in the PSS solution, and obtaining concentration is the CNT PSS solution of 40-70 mg/mL; Ultrasonic overnight treatment;
(2) with certain density HSA antibody-solutions, join in the CNT PSS solution of above-mentioned preparation, obtain HSA antibody-CNT PSS solution of HSA antibody final concentration 5-25 μ g/mL;
(3) be that the cotton thread of 5cm is immersed in HSA antibody-CNT PSS solution with length, and take out after placing 30min that 4-25 ℃ of following low temperature drying defines this and be operating as a circulation; Once more the cotton thread of drying is put in HSA antibody-CNT PSS solution and taken out drying after a period of time; After cycling 10-20 time, dry back 4 ℃ of preservations are for use, promptly prepare CNT cotton thread composite transducer, are used to monitor HSA repeatedly.
2, set up the cyclic voltammetric detection method of the electrochemical sensor of the monitoring HSA for preparing based on said method, step is:
(1) be working electrode with prepared monitoring HSA galvanochemistry cotton thread sensor, the Pt electrode is to electrode, saturated calomel electrode Hg/Hg
2Cl
2Be contrast electrode, under the control of electrochemical workstation, carry out cyclic voltammetry and measure that the record electric current is to the change curve of current potential, i.e. I-E curve;
(2) in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is noted down each I-E curve respectively;
(3) be standard with the pairing current value of 0.5 V in the I-E curve of each record, seek the corresponding relationship between electric current-HSA concentration, set up the electric current-HSA concentration standard curve that detects HSA;
(4) under identical condition, the current value of test sample liquid with the electric current of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
3, set up the resistance detection method of the galvanochemistry cotton thread sensor of the monitoring HSA for preparing based on said method, step is:
(1) is object with prepared monitoring HSA galvanochemistry cotton thread sensor, measures its resistance;
(2) in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is measured each resistance value respectively;
(3) be standard with the measured resistance value, set up the typical curve of resistance value-HSA concentration;
(4) under identical condition, the resistance value of test sample liquid with the resistance value of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
4, through using the kind of antibody in the preparation of emat sensor more, set up a kind of preparation and detection method of the universal electrochemical immunosensor based on cotton thread, the steps include:
Preparation method according to the cotton thread of human serum albumins described in the step 1 sensor; Through changing HSA antibody with corresponding haemocyanin antibody; Under identical preparation method's condition, preparation is to the universal electrochemical cotton thread immunosensor of corresponding haemocyanin monitoring usefulness.
The detection method of prepared universal electrochemical immunosensor according to the detection method of the HSA galvanochemistry cotton thread sensor described in the step 2 and 3, is set up corresponding current curve or is measured corresponding resistance value; The current value of test sample liquid or resistance value with the typical curve contrast, record the corresponding serum albumin content in the sample liquid.
The target protein that the universal electrochemical immunosensor of said cotton thread detects can be the gamma Globulin in the serum, alpha1 Globulin, alpha2 Globulin, or beta Globulin.
Beneficial effect of the present invention: provide a kind of universal fast, convenient, the preparation of high-sensitive cotton thread sensor and detection method, thereby the rapid sensitive that can be applicable to albumen in the serum detects.Especially the cotton thread that adopts can be woven into corresponding textile garment, for simple, convenient in the daily life later on and monitoring whenever and wherever possible provide possibility.Another important feature based on this sensor is, sensor prepared antibody in the process change and just can make easily to the detection of antibodies sensor of changing, and detection method also is universal detection method.
Description of drawings
Fig. 1 is based on the testing result I-E curve of the HSA sensor of preparation.* 1000, * 100, * 10, * 1 representes the extension rate of HSA respectively, and being converted into corresponding concentration is respectively 11.9 nmol/L, 119 nmol/L, 1.19 μ mol/L, 11.9 μ mol/L.
Fig. 2 based on the testing result resistance value of the HSA sensor of preparation along with the change curve of HSA variable concentrations.
Fig. 3 is based on the I-E curve and the resistance variations curve of the analysis BSA control experiment of the HSA sensor of preparation.* 1000, * 100, * 10, * 1 representes the extension rate of BSA respectively, and being converted into corresponding concentration is respectively 30 nmol/L, 300 nmol/L, 3 μ mol/L, 30 μ mol/L.
Specific embodiments
Below come the present invention is further explained (agents useful for same is a chemical pure among the embodiment) that it should be noted that following embodiment only as illustrating, content of the present invention is not limited thereto through embodiment.
The cotton thread immunity electrochemical sensor of embodiment 1 preparation human serum albumins
1, the preparation of CNT kayexalate solution: kayexalate PSS is scattered in obtains the PSS solution that mass concentration is 5%-15% in the WS; Water-soluble carbon nanometer tube is scattered in the PSS solution, and obtaining concentration is the CNT PSS solution of 40-70 mg/mL; Ultrasonic overnight treatment;
2, with certain density HSA antibody-solutions, join in the CNT PSS solution of above-mentioned preparation, obtain antibody-CNT PSS solution of HSA antibody final concentration 5-25 μ g/mL;
3, be that the cotton thread of 5cm is immersed in antibody-CNT PSS solution with length, and place after 30 minutes and take out that 4 ℃ of-25 ℃ of following low temperature dryings define this and are operating as a circulation; Once more the cotton thread of drying is put in antibody-CNT PSS solution and taken out drying after a period of time; After cycling 10-20 time, dry back 4 ℃ of preservations are for use, promptly prepare CNT cotton thread composite transducer, are used to monitor HSA repeatedly.
Embodiment 2 sets up the cyclic voltammetric detection method based on the HSA detecting sensor of said method preparation
1, be working electrode with prepared HSA cotton thread sensor, the Pt electrode is to electrode, saturated calomel electrode Hg/Hg
2Cl
2Be contrast electrode, under the control of electrochemical workstation, carry out cyclic voltammetry and measure that the record electric current is to the change curve of current potential, i.e. I-E curve;
2, in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is noted down each I-E curve respectively;
3, be standard with the pairing current value of 0.5 V in the I-E curve of each record, seek the corresponding relationship between electric current-HSA concentration, set up the electric current-HSA concentration standard curve that detects HSA;
4, under identical condition, the current value of test sample liquid with the electric current of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
1, is object with prepared HSA cotton thread sensor, measures its resistance;
2, in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is measured each resistance value respectively;
3, be standard with the measured resistance value, set up the typical curve of resistance value-HSA concentration;
4, under identical condition, the resistance value of test sample liquid with the resistance value of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
Preparation method and detection method that embodiment 4. sets up based on the universal electrochemical immunosensor of cotton thread.
1, according to the preparation method of the sensor of human serum albumins cotton thread described in the embodiment 1; Through changing HSA antibody with corresponding haemocyanin antibody; Under identical preparation method's condition, preparation is to the universal cotton thread immunosensor of corresponding haemocyanin monitoring usefulness.
2, the detection method of prepared universal electrochemical immunosensor according to the detection method of the HSA detecting sensor described in embodiment 2 and 3, is set up corresponding current curve and is measured corresponding resistance value; The current value of test sample liquid and resistance value with the typical curve contrast, record the serum albumin content in the sample liquid.
The target protein of the universal immunosensor of said cotton thread can be the gamma Globulin in the serum, alpha1 Globulin, alpha2 Globulin, beta Globulin.
Claims (5)
1. preparation method who monitors the galvanochemistry cotton thread sensor of human serum albumins is characterized in that preparation process is:
(1) preparation of CNT kayexalate solution: kayexalate PSS is scattered in obtains the PSS solution that mass concentration is 5%-15% in the WS; Water-soluble carbon nanometer tube is scattered in the PSS solution, and obtaining concentration is the CNT PSS solution of 40-70 mg/mL; Ultrasonic overnight treatment;
(2) with certain density human serum albumin HSA antibody-solutions, join in the CNT PSS solution of above-mentioned preparation, obtain HSA antibody-CNT PSS solution of HSA antibody final concentration 5-25 μ g/mL;
(3) be that the cotton thread of 5cm is immersed in HSA antibody-CNT PSS solution with length, and take out after placing 30min that 4-25 ℃ of following low temperature drying defines this and be operating as a circulation; Cotton thread that once more should drying is put in HSA antibody-CNT PSS solution and is taken out drying behind the 30min; After cycling 10-20 time, dry back 4 ℃ of preservations are for use, promptly prepare CNT cotton thread composite transducer, are used to monitor HSA repeatedly.
2. the cyclic voltammetric detection method of the galvanochemistry cotton thread sensor of the monitoring HSA of the said method of claim 1 preparation is characterized in that:
(1) be working electrode with prepared monitoring HSA galvanochemistry cotton thread sensor, the Pt electrode is to electrode, saturated calomel electrode Hg/Hg
2Cl
2Be contrast electrode, under the control of electrochemical workstation, carry out cyclic voltammetry and measure that the record electric current is to the change curve of current potential, i.e. I-E curve;
(2) in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is noted down each I-E curve respectively;
(3) be standard with the pairing current value of 0.5 V in the I-E curve of each record, seek the corresponding relationship between electric current-HSA concentration, set up electric current-HSA concentration standard curve of monitoring HSA;
(4) under identical condition, the current value of test sample liquid with the electric current of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
3. the resistance detection method of the galvanochemistry cotton thread sensor of the monitoring HAS of the said method of claim 1 preparation is characterized in that:
(1) is object with prepared monitoring HSA galvanochemistry cotton thread sensor, measures its resistance;
(2) in system, adding final concentration one by one is 11.9 nmol/L, 119 nmol/L, and 1.19 μ mol/L, the standard serial solution of 11.9 μ mol/L HSA is measured each resistance value respectively;
(3) be standard with the measured resistance value, set up the typical curve of resistance value-HSA concentration;
(4) under identical condition, the resistance value of test sample liquid with the resistance value of being set up-HSA concentration standard curve contrast, is confirmed the content of the HSA in the test sample liquid.
4. preparation method based on the universal electrochemical immunosensor of cotton thread; It is characterized in that changing the kind of using antibody in claim 1 step (2); According to the identical condition of preparation method of claim 1 monitoring human serum albumins galvanochemistry cotton thread sensor, preparation is to the universal electrochemical cotton thread immunosensor of corresponding haemocyanin monitoring usefulness.
5. with the detection method based on the universal electrochemical immunosensor of cotton thread of the said method of claim 4 preparation; It is characterized in that; With prepared universal electrochemical immunosensor is working electrode; According to the detection method of claim 2 and 3 HSA galvanochemistry cotton thread sensors, set up corresponding current curve or measure corresponding resistance value; The current value of test sample liquid or resistance value with the typical curve contrast, record the corresponding serum albumin content in the sample liquid;
The target protein that the universal electrochemical immunosensor of said cotton thread detects is a gamma Globulin, alpha1 Globulin, alpha2 Globulin, or beta Globulin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102794844A CN102426247A (en) | 2011-09-20 | 2011-09-20 | Preparation and application of universal electrochemical immunosensor based on cotton threads |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102794844A CN102426247A (en) | 2011-09-20 | 2011-09-20 | Preparation and application of universal electrochemical immunosensor based on cotton threads |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102426247A true CN102426247A (en) | 2012-04-25 |
Family
ID=45960253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102794844A Pending CN102426247A (en) | 2011-09-20 | 2011-09-20 | Preparation and application of universal electrochemical immunosensor based on cotton threads |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102426247A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983673A (en) * | 2014-05-21 | 2014-08-13 | 浙江建设职业技术学院 | Electrochemical sensor for sulfamido compound detection and preparation method and application thereof |
| CN105806922A (en) * | 2016-03-16 | 2016-07-27 | 济南大学 | Preparation method of parathion-methyl immunosensor based on combination of two electrochemical methods and application |
| CN113252749A (en) * | 2021-05-13 | 2021-08-13 | 天津理工大学 | Electrochemical test method for detecting ice crystal current and impedance |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005256222A (en) * | 2004-03-12 | 2005-09-22 | Okayama Univ | Method for coating carbon nanotube onto natural fiber |
| CN101620200A (en) * | 2009-08-03 | 2010-01-06 | 江南大学 | Preparation of universal toxin paper detection sensor and application thereof |
| CN101845750A (en) * | 2010-04-30 | 2010-09-29 | 冯静 | Manufacturing method of carbon nano tube modified fiber line and manufacturing device thereof |
| CN201660800U (en) * | 2010-04-30 | 2010-12-01 | 冯静 | Manufacturing device of carbon nanotube modified fibers |
-
2011
- 2011-09-20 CN CN2011102794844A patent/CN102426247A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005256222A (en) * | 2004-03-12 | 2005-09-22 | Okayama Univ | Method for coating carbon nanotube onto natural fiber |
| CN101620200A (en) * | 2009-08-03 | 2010-01-06 | 江南大学 | Preparation of universal toxin paper detection sensor and application thereof |
| CN101845750A (en) * | 2010-04-30 | 2010-09-29 | 冯静 | Manufacturing method of carbon nano tube modified fiber line and manufacturing device thereof |
| CN201660800U (en) * | 2010-04-30 | 2010-12-01 | 冯静 | Manufacturing device of carbon nanotube modified fibers |
Non-Patent Citations (1)
| Title |
|---|
| BONG SUP SHIM等: "Smart Electronic Yarns and Wearable Fabrics for Human Biomonitoring made by Carbon Nanotube Coating with Polyelectrolytes", 《NANO LETTERS》, vol. 8, no. 12, 11 July 2008 (2008-07-11) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983673A (en) * | 2014-05-21 | 2014-08-13 | 浙江建设职业技术学院 | Electrochemical sensor for sulfamido compound detection and preparation method and application thereof |
| CN105806922A (en) * | 2016-03-16 | 2016-07-27 | 济南大学 | Preparation method of parathion-methyl immunosensor based on combination of two electrochemical methods and application |
| CN105806922B (en) * | 2016-03-16 | 2018-05-08 | 济南大学 | A kind of preparation method and application based on parathion-methyl immunosensor associated with two kinds of electrochemical methods |
| CN113252749A (en) * | 2021-05-13 | 2021-08-13 | 天津理工大学 | Electrochemical test method for detecting ice crystal current and impedance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sagadevan et al. | Recent trends in nanobiosensors and their applications-a review | |
| Huang et al. | Amperometric immunobiosensor for α-fetoprotein using Au nanoparticles/chitosan/TiO2–graphene composite based platform | |
| Park et al. | Applications of field-effect transistor (FET)-type biosensors | |
| Zheng et al. | Simultaneous determination of ascorbic acid, dopamine and uric acid using poly (l-leucine)/DNA composite film modified electrode | |
| Hou et al. | Ultratrace detection of glucose with enzyme-functionalized single nanochannels | |
| US8926822B2 (en) | Systems and methods for integrated electrochemical and electrical detection | |
| Wu et al. | Paper supported immunosensor for detection of antibiotics | |
| CN101655473B (en) | Preparation method of nanogold immunoelectrode | |
| Zhang et al. | An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells | |
| Zhou et al. | Carbon nanospheres-promoted electrochemical immunoassay coupled with hollow platinum nanolabels for sensitivity enhancement | |
| Wang et al. | Ultra-sensitive and rapid screening of acute myocardial infarction using 3D-affinity graphene biosensor | |
| CN103616423A (en) | Preparation method and application of competitive type aptamer sensor for detecting oxytetracycline | |
| Zhang et al. | Chemically functionalized conical PET nanopore for protein detection at the single-molecule level | |
| CN102706939A (en) | Electrochemical immunosensor for directly detecting IL-6 (interleukin-6) antigen and application thereof | |
| Wang et al. | An amperometric sensor for nitric oxide based on a glassy carbon electrode modified with graphene, Nafion, and electrodeposited gold nanoparticles | |
| CN102426247A (en) | Preparation and application of universal electrochemical immunosensor based on cotton threads | |
| Chen et al. | Fabrication of PEDOT nanowhiskers for electrical connection of the hemoglobin active center for H 2 O 2 electrochemical biosensing | |
| CN103224925A (en) | Ferrocene polypeptide nanowire-glucose oxidizing enzyme composite and preparing and applying method thereof | |
| Zhang et al. | A facile integrated microfluidic chip based on Chitosan-Gold Nanoparticles-Anchored Three-Dimensional graphene fiber film for monitoring prostate specific antigen | |
| Businova et al. | Voltammetric sensor for direct insulin detection | |
| CN106442664A (en) | Pyocyanin bioelectricity chemical detection method | |
| Luo et al. | A novel electrochemical immunosensor for detection of angiotensinII at a glass carbon electrode modified by carbon nanotubes/chitosan film | |
| Xie et al. | An electrochemical aptasensor for Mycobacterium tuberculosis ESAT-6 antigen detection using bimetallic organic framework | |
| Wang et al. | Fabrication of electrochemical immunosensor for interferon-γ determination and its application of tuberculosis diagnosis | |
| Shamsipur et al. | Single frequency impedance strategy employed in rapid detection of leukemia cancer cells using an electrospun PES-nanofiber reinforced ternary composite-based cytosensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120425 |