CN102391390A - Tobacco extraction method based on steam explosion - Google Patents
Tobacco extraction method based on steam explosion Download PDFInfo
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Abstract
The invention relates to a chemical extraction technology, and especially relates to a method for extracting pectin from tobacco. The method comprises specific steps that: tobacco is crushed, such that tobacco powder is obtained; the tobacco powder is soaked by using water for at least 8 hours, and is processed through steam explosion for at least 60 seconds under a pressure of 0.04 to 0.3Mpa, such that a tobacco extract is obtained; an acid is added to the tobacco extract, and a pH value of the solution is regulated to 1.0 to 3.0; the solution is completely hydrolyzed under a temperature of 70 to 90 DEG C; residues are removed, such that a hydrolysate is obtained; the hydrolysate obtained in the step A is precipitated by using a ethanol precipitation method, and the obtained precipitate is pectin. Compared to a traditional method wherein a tobacco material is boiled by using boiled water, and wherein pectinase is passivated, the tobacco extract preparation method provided by the invention is advantaged in low pollution, low energy consumption, and complete pectinase passivation. With themethod provided by the invention, 1 hour can be saved, and the yield of pectin can reach approximately 12.2%, which is increased by 3.2% comparing to that of a traditional ethanol extraction method. Meanwhile, the color of the obtained pectin is good.
Description
Technical field
What the present invention relates to extracts to chemical technology, particularly from tobacco, extracts the method for compound.
Background technology
Tobacco is meant depleted tobacco leaf, offal etc. in the tobacco production process, and tobacco generally accounts for the 15-20% of raw tobacco total amount.China is tobacco big country, has every year few hundred thousand tonnes of offal resource to go out of use, and has both caused environmental pollution, also is the waste to natural resources simultaneously.Many researchs have been carried out in the comprehensive utilization of tobacco, and for example extraction separation goes out fragrance material, nicotine, protein and organic acid etc. from tobacco leaf, and this has opened up wide prospect for the comprehensive utilization of tobacco.Pectin is the distinctive component of plant cell wall, is the hydrophilic colloid material, is substruction with D-galacturonic acid glycan.The osmotic absorption moisture of pectin, influential to the water absorbability and the elasticity of tobacco leaf.The content of pectin in tobacco leaf is different because of the difference of kind, and pectin content is usually about 10% in the flue-cured tobacco.Extracting pectin mainly comprises 1) sour formulation: its cardinal principle is that the protopectin in the vegetable cell is changed into water soluble pectin and extracts under the acid solution effect.Xiao Hourong discloses the optimised process that pectin extracts in " from offal, extracting the pectin technical study " literary composition: nitration mixture pH value is about 0.5, and 60 minutes extraction time, extraction temperature is 90 ℃, and the proportioning of material and nitration mixture is 1:30.Pectin solution and ethanol solution volume ratio are 1:1-2:3; But this method pectin yield is low, and easily because of the improper degraded that causes pectin of pH value control.2) ultrasonic-assisted extraction method: when the sound wave of ultrasonic frequency more than 20KHz, when ultrasonic vibration, produce and transmit powerful ability, cause that the medium particle gets into vibrational state with friction-motion speed, promote effective constituent to dissolve.Application number is that 201010252413.0 Chinese invention patent application discloses a kind of steam explosion offal that utilizes to improve the method for its sucking quality, and purpose is to improve the offal sucking quality, improves the utilization ratio of offal in production of cigarettes; Effectively reduce the cigarette cost; Concrete grammar is: to polygalacturonase, cellulase, hemicellulase, zytase, and laccase, glycase and saccharifying enzyme carry out composite; Convert the suitable water yield, then offal after the explosion is carried out enzyme and spray; Control offal moisture is 40～50% in whole process, and treatment temp is 40～50 ℃, and the Terrier Storage time is 4～10h; At last the offal after handling is carried out 70～90 ℃, 20～40min enzyme that goes out.But the method for above-mentioned steam explosion must be handled by enzyme, and purpose is to improve the offal sucking quality.Except pectin, the Study on Extraction Method of the Mierocrystalline cellulose in the tobacco, semicellulose and xylogen is also less, and it is harmful to environment to relate to extraction reagent.
Summary of the invention
One of the object of the invention is to provide a kind of pretreated method of tobacco in the pectin technology of from tobacco, extracting, and this method can broken to greatest extent tobacco cell material, the passivation polygalacturonase.
The preparation method of tobacco extract, concrete steps are: after tobacco is pulverized powder, fully soak with water medium, be to be not less than 60 seconds with steam explosion under the condition of 0.04-0.3Mpa at pressure, tobacco extract.
Further, the preparation method of tobacco extract soaked 12 hours with the water medium that is equivalent to powder 10-30 times volume.
Two of the object of the invention is to provide a kind of pectin extract raw material, and this raw material is handled and got through steam explosion, and its pectin yield is higher than traditional raw material.
The tobacco extract of preparing method's preparation of described tobacco extract.
Three of the object of the invention is to provide a kind of extracting pectin, and this method is to utilize the tobacco waste residue to be raw material, and its yield is high, and the process time is short.
For realizing above-mentioned purpose, technical scheme of the present invention is:
Use described tobacco extract to extract the method for pectin, specifically may further comprise the steps:
The A acid hydrolysis
Described tobacco extract is added acid for adjusting pH value to 1.0-3.0, is the reaction that fully is hydrolyzed under the 70-90 ℃ of condition in temperature, removes residue, gets hydrolyzed solution;
The B ethanol sedimentation
Steps A gained hydrolyzed solution is precipitated the said pectin that is precipitated as with ethanol precipitation.
Further, with the processing of decolouring of steps A gained hydrolyzed solution.
Further, steps A gained hydrolyzed solution is used the charcoal absorption decolouring of massfraction as 2-5%, bleaching temperature is 50-60 ℃.
Further, said acid hydrolysis is under power is not less than the UW of 10kHz, to carry out.
Further, in step B, steps A gained hydrolyzed solution is concentrated into the 10-20% of original volume, liquid concentrator, using massfraction is that 95% ethanol precipitates said liquid concentrator, the volume ratio 1:20 of said ethanol and liquid concentrator removes ethanol, must pectin.
Further, use massfraction to be 50% washing with alcohol the step B gained deposition after, dehydration, dry and pulverize after pectin.
Further, with the tobacco extract filtering separation, collecting filter residue and carry out drying, dried filter residue is added water medium, add acid for adjusting pH value to 1.0-3.0, is the reaction that fully is hydrolyzed under the 70-90 ℃ of condition in temperature, removes residue, gets hydrolyzed solution.
Four of the object of the invention is to provide respectively the preparation method of semicellulose, Mierocrystalline cellulose and xylogen, and this method is to utilize the tobacco waste residue to be raw material, and its yield is high, and the process time is short.
For realizing above-mentioned technical purpose, technical scheme of the present invention is:
Use described tobacco extract to prepare the method for semicellulose, specifically may further comprise the steps: said tobacco extract is filtered, must filtrate; Said filtrating is regulated pH value 3.0-5.0; After waiting to produce solid precipitation, carry out liquid-solid separation, said solid precipitation is a semicellulose.
Further, said tobacco extract filters, and goes filtrating; Said filtrating is regulated pH value 3.0-5.0, after treating fully to produce solid precipitation, carry out liquid-solid separation; Collect solid precipitation, the liquid after the liquid-solid separation precipitates with ethanol precipitation, collecting precipitation; Said solid precipitation and said deposition are merged, must merge thing, said merging thing is a semicellulose.
Use described tobacco extract to prepare cellulosic method; Specifically may further comprise the steps: in said tobacco extract, add and be equivalent to tobacco powder quality 10-15 1-butyl-3-Methylimidazole villaumite doubly; Under 80-100 ℃ of condition, carry out sufficient solubilizing reaction, get reaction solution, said reaction solution is carried out solid-liquid separation; Get liquid, said liquid is cellulose solution; In the gained cellulose solution, add water medium, the volume ratio of said cellulose solution and said water medium is 1::3-5, filters after stirring is no less than 30 minutes, and the gained filter residue is a Mierocrystalline cellulose.
Use described tobacco extract to prepare the method for xylogen; Specifically may further comprise the steps: said tobacco extract is filtered, must filtrate, said filtrating is evaporative removal 1-butyl-3-Methylimidazole villaumite under 100-120 ℃ of condition; Remaining liq with acid for adjusting pH value to 4-5; And under 70-80 ℃ of condition, reacted 40-90 minute, remove liquid phase after cooling, remaining solid is an xylogen.
Beneficial effect of the present invention is: 1 boils tobacco passivation polygalacturonase with boiling water and compares with traditional, and the preparation method of tobacco extract has and pollutes for a short time, and power consumption is few, and the polygalacturonase passivation is advantage completely; 2 adopt tobacco extract to extract pectin, on average can practice thrift 1 hour time, can also obtain higher pectin yield; 3 adopt the steam explosion preconditioning technique to extract pectin, and the productive rate of pectin can reach about 12.2%, has improved 3.2% than traditional ethanol extraction method, and the color and luster of pectin is better simultaneously, is convenient to further purification.Adopting the yield of present method semicellulose is 90.4%, and cellulosic yield is 90.2%, and the yield of xylogen is 92.5%.
More beneficial effect will embody in an embodiment.
Embodiment
The tobacco of indication among the present invention; Also comprise offal, tobacco stem and tobacco leaf, depleted tobacco during especially tobacco produces, the tobacco among the following embodiment come from Chongqing silver good fortune biological organic fertilizer ltd; For tobacco produces waste, its water cut is 7% of a massfraction.
The decolouring of indication is handled and is specially among the present invention: the color and luster of pectin has considerable influence to the quality of pectin, and the pectin that from plant, extracts has darker color, and produce fine pectin must decolour to hydrolyzed solution before being settled out pectin.The method that decolouring is handled comprises methods such as activated carbon decolorizing, ydrogen peroxide 50 decolouring, IX.
The principle of the ethanol precipitation precipitated pectin of indication is among the present invention: utilize the aldehyde radical that contains in the pectin (CHO) with in the ethanol to contain the hydroxyl (precipitin reaction that OH) reacts and form.
The principle of the ethanol precipitation of indication deposition semicellulose is among the present invention: semicellulose is the most complicated component in the cell walls of timber and non-wood plant.They are connected with Mierocrystalline cellulose through hydrogen bond, are connected with covalent linkage (mainly being the phenmethyl ehter bond) with xylogen, are connected with ester bond with hydroxycinnamic acid with the ethanoyl unit.Semicellulose is under sour environment, and hydrogen bond ruptures, and semicellulose is similar with the low mass molecule alcohol class, and according to the similar compatibility principle, semicellulose is dissolved in the ethanol, reclaims ethanol through rotary evaporation again, obtains the semicellulose solid.
Being calculated as of yield among the present invention: being calculated as of pectin yield among the following embodiment: pectin content=(galacturonic acid concentration/example weight) * 500 * 100 %, pectin yield are=(the pectin amount/raw material pectin content of extraction) * 100%
The preparation of embodiment 1 tobacco extract
After the 10g tobacco pulverized powder, soaked 8 hours with the deionized water that is equivalent to 10 times of volumes of powder, at pressure be under the condition of 0.04Mpa with steam explosion 60 seconds, get tobacco extract; The extraction of tobacco semicellulose: use HCL to regulate filtrating pH value and be 3.0, centrifugal, residue is a semicellulose; It is 95% ethanol precipitating, centrifugal that suspension-s after centrifugal uses volume(tric)fraction, and residue is a semicellulose, merges the gained semicellulose twice; The dried scarp of steam explosion is used to extract pectin.
The preparation of embodiment 2 tobacco extracts
After the 10g tobacco pulverized powder, soaked 8 hours with the water medium that is equivalent to 30 times of volumes of powder, at pressure be under the condition of 0.3Mpa with steam explosion 180 seconds, get tobacco extract; The extraction of tobacco semicellulose: use HCL to regulate filtrating pH value and be 5.0, centrifugal, residue is a semicellulose; Suspension-s after centrifugal is with the ethanol precipitating of volume(tric)fraction 95%, centrifugal, and residue is a semicellulose, merges semicellulose twice; The dried scarp of steam explosion is used to extract pectin.
Embodiment 3 ethanol extraction methods extract pectin
Ethanol extraction method extracts pectin; Be divided into is 3 groups; First group raw material is embodiment 1 a gained tobacco extract, and second group raw material is embodiment 2 gained tobacco extracts, and the 3rd group preparation method is: get powder after the 10g tobacco is pulverized; Soaked 8 hours with the boiling water that is equivalent to 10 times of volumes of powder, get tobacco extract; The extraction of tobacco semicellulose: use HCL to regulate filtrating pH value and be 4.0, centrifugal, residue is a semicellulose; It is 95% ethanol precipitating, centrifugal that suspension-s after centrifugal uses volume(tric)fraction, and residue is a semicellulose, merges semicellulose twice.
The concrete steps of ethanol extraction method are: respectively three groups of tobacco extracts are filtered; Collect the filter residue in the tobacco extract,, in dried filter residue, add the deionized water that is equivalent to 10 times of volumes of filter residue in 80 ℃ of dryings 8 hours; Using the salt acid for adjusting pH value is 1.0; In temperature is the reaction 120 minutes that fully is hydrolyzed under 70 ℃ of conditions, removes residue, gets hydrolyzed solution.It is 3% charcoal absorption decolouring 60 minutes that hydrolyzed solution is used massfraction, and bleaching temperature is 50 ℃.To be concentrated into 15% of hydrolyzed solution original volume with Rotary Evaporators through the hydrolyzed solution after the charcoal absorption decolouring is handled, get liquid concentrator.Liquid concentrator is precipitated with ethanol precipitation: using the massfraction be equivalent to 20 times of volumes of liquid concentrator is that 95% ethanol sedimentation was handled collecting precipitation, the said pectin that is precipitated as 6 hours; To precipitate use volume(tric)fraction to be 50% washing with alcohol after, dewatering, be dried to water cut is that pulverize 2% back.
To detect, the yield of first group of pectin is that the yield of 10.2%, the second group of pectin is that the yield of 10.4%, the three group of pectin is 9.2%.
Embodiment 4 acid-hydrolysis methods extract pectin
Acid-hydrolysis method extracts pectin; Be divided into is 3 groups; First group raw material is embodiment 1 a gained tobacco extract, and second group raw material is embodiment 2 gained tobacco extracts, and the 3rd group preparation method is: get tobacco powder after the 10g tobacco is pulverized; Boiling water immersion with being equivalent to 30 times of volumes of tobacco powder is no less than 0.2 hour, gets tobacco extract.
The volume ratio of powder in the tobacco extract and deionized water is adjusted into 1::1, and using the salt acid for adjusting pH value is 1.0, is the reaction 60 minutes that fully is hydrolyzed under 70 ℃ of conditions in temperature, removes residue, gets hydrolyzed solution.It is 3% charcoal absorption decolouring 60 minutes that hydrolyzed solution is used massfraction, and bleaching temperature is 50 ℃.To under 80 ℃ of conditions, be concentrated into 15% of hydrolyzed solution original volume with Rotary Evaporators through the hydrolyzed solution after the charcoal absorption decolouring is handled, get liquid concentrator.Liquid concentrator is precipitated with ethanol precipitation: using the massfraction be equivalent to 20 times of volumes of liquid concentrator is that 95% ethanol sedimentation was handled collecting precipitation, the said pectin that is precipitated as 6 hours; To precipitate use volume(tric)fraction to be 50% washing with alcohol after, dewatering, be dried to water cut is that pulverize 2% back.
To detect, the yield of first group of pectin is that the yield of 9.8%, the second group of pectin is that the yield of 10.1%, the three group of pectin is 8.9%.
Embodiment 5 ultrasonic methods are extracted pectin
Ultrasonic method is extracted pectin; Be divided into is 3 groups; First group raw material is embodiment 1 a gained tobacco extract, and second group raw material is embodiment 2 gained tobacco extracts, and the 3rd group preparation method is: get powder after the 10g tobacco is pulverized; Boiling water immersion with being equivalent to 30 times of volumes of tobacco powder is no less than 0.1 hour, gets tobacco extract.
Tobacco extract is filtered and washs once, and the volume ratio of tobacco powder in the tobacco extract and deionized water is adjusted into 1::1, using the salt acid for adjusting pH value is 1.0; At ultrasonic power is 40kHz; Temperature is the reaction 30 minutes that fully is hydrolyzed under 70 ℃ of conditions, removes residue, gets hydrolyzed solution.It is 3% charcoal absorption decolouring 60 minutes that hydrolyzed solution is used massfraction, and bleaching temperature is 50 ℃.To be concentrated into 20% of hydrolyzed solution original volume with Rotary Evaporators 90 ℃ of conditions through the hydrolyzed solution after the charcoal absorption decolouring is handled, get liquid concentrator.Liquid concentrator is precipitated with ethanol precipitation: using the massfraction be equivalent to 20 times of volumes of liquid concentrator is that 95% ethanol sedimentation was handled collecting precipitation, the said pectin that is precipitated as 6 hours; To precipitate use volume(tric)fraction to be 50% washing with alcohol after, dewatering, be dried to water cut is that pulverize 2% back.
Through detecting, the yield of first group of pectin is that the yield of 11.8%, the second group of pectin is that the yield of 12.3%, the three group of pectin is 8.7%.
The extraction of embodiment 6 semicelluloses
The preparation method of 1 tobacco extract, concrete steps are: after the 100g tobacco is pulverized powder, soaked 8 hours with water medium, at pressure be under the condition of 0.04Mpa with steam explosion 60 seconds, must tobacco extract 1.Said tobacco extract filters, and goes filtrating, and said filtrating is regulated pH value 3.0; After treating fully to produce solid precipitation, carry out liquid-solid separation, collect solid precipitation; It is that 95% ethanol precipitation precipitates that liquid after the liquid-solid separation uses massfraction, and collecting precipitation merges said solid precipitation and said deposition; Must merge thing, said merging thing is a semicellulose.Get tobacco powder after simultaneously the 100g tobacco being pulverized, soaked 8 hours, must contrast tobacco extract 1 with water medium.The method for preparing semicellulose is identical.
Tobacco extract 1 is 83.5% for the yield of the semicellulose of raw material, is that the yield of the semicellulose of raw material is 33.4% with contrast tobacco extract 1.Hemicellulose level is about 14% in the tobacco, and we are research object with the 100g material here, and then semicellulose is 14g. (semicellulose yield=obtain semicellulose amount/semicellulose total amount) * 100%.
The preparation method of 2 tobacco extracts, concrete steps are: after the 100g tobacco is pulverized powder, soaked 12 hours with deionized water, at pressure be under the condition of 0.3Mpa with steam explosion 180 seconds, must tobacco extract 2.Said tobacco extract filters, and goes filtrating, and said filtrating is regulated pH value 5.0; After treating fully to produce solid precipitation, carry out liquid-solid separation, collect solid precipitation; It is that 95% ethanol precipitation precipitates that liquid after the liquid-solid separation uses massfraction, and collecting precipitation merges said solid precipitation and said deposition; Must merge thing, said merging thing is a semicellulose.
Tobacco extract 1 is 90.4% for the yield of the semicellulose of raw material.
Embodiment 7 cellulosic extractions
The preparation method of 1 tobacco extract, concrete steps are: after the 100g tobacco is pulverized powder, soaked 8 hours with water medium, at pressure be under the condition of 0.04Mpa with steam explosion 60 seconds, must tobacco extract 1.In said tobacco extract, add the 1-butyl-3-Methylimidazole villaumite that is equivalent to 10 times of tobacco qualities, under 80 ℃ of conditions, carry out sufficient solubilizing reaction, get reaction solution, said reaction solution is carried out solid-liquid separation, get liquid, said liquid is cellulose solution; In the gained cellulose solution, add deionized water, the volume ratio of said cellulose solution and said water medium is 1::5,, stir and filter after 30 minutes, the gained filter residue is a Mierocrystalline cellulose.Get tobacco powder after simultaneously the 100g tobacco being pulverized, soaked 8 hours, must contrast tobacco extract 1 with water medium.It is identical to prepare cellulosic method.
Tobacco extract 1 is for the cellulosic yield of raw material is 89.5%, is that the cellulosic yield of raw material is 30.6% with contrast tobacco extract 1.Content of cellulose is about 22% in the tobacco, and we are research object with the 100g material here, and then Mierocrystalline cellulose is 22g. (Mierocrystalline cellulose yield=obtain cellulose amount/Mierocrystalline cellulose total amount) * 100%.
The preparation method of 2 tobacco extracts, concrete steps are: after the 100g tobacco is pulverized tobacco powder, soaked 12 hours with water medium, at pressure be under the condition of 0.3Mpa with steam explosion 180 seconds, must tobacco extract 2.In said tobacco extract, add the 1-butyl-3-Methylimidazole villaumite that is equivalent to 15 times of tobacco qualities, under 100 ℃ of conditions, carry out sufficient solubilizing reaction, get reaction solution, said reaction solution is carried out solid-liquid separation, get liquid, said liquid is cellulose solution; In the gained cellulose solution, add deionized water, the volume ratio of said cellulose solution and deionized water is 1:3, stirs and filters after 60 minutes, and the gained filter residue is a Mierocrystalline cellulose.Cellulose regenerated principle: the Mierocrystalline cellulose that is dissolved in the ionic liquid can go out through adding elutriation, and this kind solvent more is prone to ionic liquid formation hydrogen bond the Mierocrystalline cellulose back extraction come out thereby this is.Because ionic liquid is cellulosic direct solvent, rapid precipitation can prevent that the dissolved Mierocrystalline cellulose from returning to original crystalline state simultaneously.
Cellulosic yield is 92.5%.
The extraction of embodiment 8 xylogen
The preparation method of 1 tobacco extract, concrete steps are: after the 100g tobacco is pulverized powder, soaked 8 hours with water medium, at pressure be under the condition of 0.04Mpa with steam explosion 60 seconds, must tobacco extract 1.Get tobacco powder after simultaneously the 100g tobacco being pulverized, soaked 8 hours, must contrast tobacco extract 1 with deionized water.Said tobacco extract is filtered, must filtrate, said filtered liq is evaporative removal 1-butyl-3-Methylimidazole villaumite under 100 ℃ of conditions; Remaining liq is with acid for adjusting pH value to 4; And under 70 ℃ of conditions, reacted 40 minutes, remove liquid phase after cooling, remaining solid is an xylogen.
Tobacco extract 1 is 38.9% for the yield of the xylogen of raw material, is that the yield of the xylogen of raw material is 89.6% with contrast tobacco extract 1.Content of lignin is about 7% in the tobacco, and we are research object with the 100g material here, and then xylogen is 7g. (xylogen yield=obtain xylogen amount/xylogen total amount) * 100%.
The preparation method of 2 tobacco extracts, concrete steps are: after the 100g tobacco is pulverized powder, soaked 12 hours with deionized water, at pressure be under the condition of 0.3Mpa with steam explosion 180 seconds, must tobacco extract 2.Said tobacco extract is filtered, must filtrate, said filtered liq is evaporative removal 1-butyl-3-Methylimidazole villaumite under 120 ℃ of conditions; Remaining liq is with acid for adjusting pH value to 5; And under 80 ℃ of conditions, reacted 90 minutes, remove liquid phase after cooling, remaining solid is an xylogen.
Tobacco extract 2 is 90.2% for the yield of the xylogen of raw material
Acid for adjusting pH value in the foregoing description, said acid all refers to hydrochloric acid, also can use such as other acid such as sulfuric acid to substitute.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although through invention has been described with reference to the preferred embodiments of the present invention; But those of ordinary skill in the art should be appreciated that and can make various changes to it in form with on the details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (14)
1. the preparation method of tobacco extract is characterized in that, concrete steps are: after tobacco is pulverized powder, fully soak with water medium, be to be not less than 60 seconds with steam explosion under the condition of 0.04-0.3Mpa at pressure, tobacco extract.
2. the preparation method of tobacco extract is characterized in that, soaks 12 hours with the water medium that is equivalent to powder 10-30 times volume.
3. the tobacco extract of the preparation method of the described tobacco extract of claim 1 preparation.
4. the described tobacco extract of utilization claim 3 extracts the method for pectin, it is characterized in that, specifically may further comprise the steps:
The A acid hydrolysis
Described tobacco extract is added acid for adjusting pH value to 1.0-3.0, is the reaction that fully is hydrolyzed under the 70-90 ℃ of condition in temperature, removes residue, gets hydrolyzed solution;
The B ethanol sedimentation
Steps A gained hydrolyzed solution is precipitated the said pectin that is precipitated as with ethanol precipitation.
5. tobacco extract according to claim 4 extracts the method for pectin, it is characterized in that, with the processing of decolouring of steps A gained hydrolyzed solution.
6. tobacco extract according to claim 5 extracts the method for pectin, it is characterized in that, steps A gained hydrolyzed solution is used the charcoal absorption decolouring of massfraction as 2-5%, and bleaching temperature is 50-60 ℃.
7. tobacco extract according to claim 4 extracts the method for pectin, it is characterized in that, said acid hydrolysis is under power is not less than the UW of 10kHz, to carry out.
8. tobacco extract according to claim 4 extracts the method for pectin, it is characterized in that, in step B; Steps A gained hydrolyzed solution is concentrated into the 10-20% of original volume; Liquid concentrator, using massfraction is that 95% ethanol precipitates the volume ratio 1:20 of said ethanol and liquid concentrator to said liquid concentrator; Remove ethanol, get pectin.
9. tobacco extract according to claim 4 extracts the method for pectin, it is characterized in that: after using massfraction to be 50% washing with alcohol step B gained deposition, dehydration, dry and pulverize after pectin.
10. tobacco extract according to claim 4 extracts the method for pectin; It is characterized in that: with said tobacco extract filtering separation, collect filter residue and carry out drying, dried filter residue is added water medium; Add acid for adjusting pH value to 1.0-3.0; In temperature is the reaction that fully is hydrolyzed under the 70-90 ℃ of condition, removes residue, gets hydrolyzed solution.
11. the described tobacco extract of utilization claim 3 prepares the method for semicellulose; It is characterized in that, specifically may further comprise the steps: said tobacco extract is filtered, must filtrate; Said filtrating is regulated pH value 3.0-5.0; After waiting to produce solid precipitation, carry out liquid-solid separation, said solid precipitation is a semicellulose.
12. tobacco extract according to claim 11 prepares the method for semicellulose, it is characterized in that, said tobacco extract filters, and goes filtrating; Said filtrating is regulated pH value 3.0-5.0, after treating fully to produce solid precipitation, carry out liquid-solid separation; Collect solid precipitation, the liquid after the liquid-solid separation precipitates with ethanol precipitation, collecting precipitation; Said solid precipitation and said deposition are merged, must merge thing, said merging thing is a semicellulose.
13. the described tobacco extract of utilization claim 3 prepares cellulosic method; It is characterized in that, specifically may further comprise the steps: in said tobacco extract, add and be equivalent to tobacco powder quality 10-15 1-butyl-3-Methylimidazole villaumite doubly, under 80-100 ℃ of condition, carry out sufficient solubilizing reaction; Get reaction solution; Said reaction solution is carried out solid-liquid separation, get liquid, said liquid is cellulose solution; In the gained cellulose solution, add water medium, the volume ratio of said cellulose solution and said water medium is 1:3-5, filters after stirring is no less than 30 minutes, and the gained filter residue is a Mierocrystalline cellulose.
14. the described tobacco extract of utilization claim 3 prepares the method for xylogen, it is characterized in that, specifically may further comprise the steps: said tobacco extract is filtered; Must filtrate; Said filtrating is evaporative removal 1-butyl-3-Methylimidazole villaumite under 100-120 ℃ of condition, remaining liq with acid for adjusting pH value to 4-5, and under 70-80 ℃ of condition the reaction 40-90 minute; Remove liquid phase after cooling, remaining solid is an xylogen.
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| CN108347994A (en) * | 2015-09-08 | 2018-07-31 | R.J.雷诺兹烟草公司 | The high pressure low temperature pasteurize of tobacco-containing material |
| CN109998149A (en) * | 2019-05-08 | 2019-07-12 | 江苏中烟工业有限责任公司 | A kind of economical recombination stem processing method |
| CN110041446A (en) * | 2019-03-20 | 2019-07-23 | 肖兵 | A kind of biomass comprehensive method for refining |
| CN110483187A (en) * | 2019-09-24 | 2019-11-22 | 成都市四友生物科技有限公司 | A kind of tobacco powder biological organic fertilizer inhibiting crop disease and insect |
| CN111333070A (en) * | 2020-03-10 | 2020-06-26 | 天津科技大学 | Method for utilizing all components of sunflower disc raw material |
| CN113322355A (en) * | 2021-05-31 | 2021-08-31 | 华南理工大学 | Automatic quality control method for cellulosic ethanol blasting pretreatment process |
| CN113508919A (en) * | 2020-12-31 | 2021-10-19 | 上海零诺生物科技有限公司 | Tobacco extract and preparation method and application thereof |
| CN114264618A (en) * | 2021-11-15 | 2022-04-01 | 中国烟草总公司福建省公司 | Method for detecting starch content in tobacco |
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| CN102697167B (en) * | 2012-06-25 | 2014-04-16 | 湖北中烟工业有限责任公司 | Preparation method for tobacco stem extract with assistance of steam explosion |
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| US20150296873A1 (en) * | 2012-11-26 | 2015-10-22 | British American Tobacco (Investments) Limited | Treatment of tobacco material |
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| JP2015536149A (en) * | 2012-11-26 | 2015-12-21 | ブリティッシュ アメリカン タバコ （インヴェストメンツ） リミテッドＢｒｉｔｉｓｈ Ａｍｅｒｉｃａｎｔｏｂａｃｃｏ （Ｉｎｖｅｓｔｍｅｎｔｓ） Ｌｉｍｉｔｅｄ | Tobacco material processing |
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| CN104725528A (en) * | 2015-03-31 | 2015-06-24 | 川渝中烟工业有限责任公司 | Method for extracting pectin from tobacco waste by utilizing flash extracting technology |
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| CN105167174A (en) * | 2015-07-16 | 2015-12-23 | 华南理工大学 | Leaching liquor capable of effectively reducing lignin content in tobacco waste as well as preparation method and application of leaching liquor |
| CN108347994A (en) * | 2015-09-08 | 2018-07-31 | R.J.雷诺兹烟草公司 | The high pressure low temperature pasteurize of tobacco-containing material |
| CN108347994B (en) * | 2015-09-08 | 2021-09-03 | R.J.雷诺兹烟草公司 | High pressure low temperature pasteurization of tobacco material |
| CN105433433A (en) * | 2015-10-31 | 2016-03-30 | 武汉纽威晨创科技发展股份有限公司 | Method for extracting tobacco aromatic components through water jet smashing and centrifugal membrane microwave distillation |
| CN105433433B (en) * | 2015-10-31 | 2017-03-22 | 武汉纽威晨创科技发展股份有限公司 | Method for extracting tobacco aromatic components through water jet smashing and centrifugal membrane microwave distillation |
| CN106087507A (en) * | 2016-06-02 | 2016-11-09 | 华南理工大学 | Beet pulp cellulose Nanowire and preparation method thereof |
| CN106087507B (en) * | 2016-06-02 | 2018-04-20 | 华南理工大学 | Beet pulp cellulose Nanowire and preparation method thereof |
| CN110041446A (en) * | 2019-03-20 | 2019-07-23 | 肖兵 | A kind of biomass comprehensive method for refining |
| CN109998149A (en) * | 2019-05-08 | 2019-07-12 | 江苏中烟工业有限责任公司 | A kind of economical recombination stem processing method |
| CN110483187A (en) * | 2019-09-24 | 2019-11-22 | 成都市四友生物科技有限公司 | A kind of tobacco powder biological organic fertilizer inhibiting crop disease and insect |
| CN111333070A (en) * | 2020-03-10 | 2020-06-26 | 天津科技大学 | Method for utilizing all components of sunflower disc raw material |
| CN111333070B (en) * | 2020-03-10 | 2022-09-16 | 天津科技大学 | Method for utilizing whole components of sunflower disc raw material |
| CN113508919A (en) * | 2020-12-31 | 2021-10-19 | 上海零诺生物科技有限公司 | Tobacco extract and preparation method and application thereof |
| CN113322355A (en) * | 2021-05-31 | 2021-08-31 | 华南理工大学 | Automatic quality control method for cellulosic ethanol blasting pretreatment process |
| CN114264618A (en) * | 2021-11-15 | 2022-04-01 | 中国烟草总公司福建省公司 | Method for detecting starch content in tobacco |
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