CN102391384A - Production process for high acyl gellan gum - Google Patents
Production process for high acyl gellan gum Download PDFInfo
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- CN102391384A CN102391384A CN2011102973045A CN201110297304A CN102391384A CN 102391384 A CN102391384 A CN 102391384A CN 2011102973045 A CN2011102973045 A CN 2011102973045A CN 201110297304 A CN201110297304 A CN 201110297304A CN 102391384 A CN102391384 A CN 102391384A
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- gellan gum
- acyl gellan
- high acyl
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- colloid
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Abstract
The invention provides a production process for high acyl gellan gum. The high acyl gellan gum is prepared by the processes of heating and filtering fermentation liquid, precipitating by using organic solvents, separating solid from liquid, decoloring, dehydrating, drying, crushing and the like. An extraction process for the high acyl gellan gum is simple and novel, low in production cost, high in product quality and more suitable for industrialized production.
Description
Technical field
The present invention relates to a kind of high acyl gellan gum production technique.
Background technology
Gelling gum is a kind of mikrobe edible gum of the U.S. Kelco company exploitation eighties in 20th century, and its gellifying property is more more superior than XG 550.It is by sphingomonas paucimobilis under neutrallty condition; With glucose is carbon source; The extracellular polysaccharide colloid that produces through aerobic fermentation is a kind of novel bright gelifying agent of full impregnated, and China has ratified gelling gum in 1996 and used in food as foodstuff additive.
The substruction of gelling gum molecule is a main chain, is made up of multiple tetrose unit, and the monose of participating in forming has glucose, glucuronic acid and rhamnosyl, mol ratio 2:1:1.Usually the gelling gum product is divided into two kinds: a kind of is the high acyl gellan gum product, is prone to form viscoelastic gel after the aquation.What normal gellan gum fermentation liquid was contained is high acyl gellan gum, the C of the D-glucosyl residue in the repeating unit of other polysaccharide molecule
6Have an appointment and 50% replaced by ethanoyl; Another kind is the low-acyl gellan gum product, through high acyl gellan gum being made the deacylated tRNA base or part deacylated tRNA base obtains, under cation sites, very easily forms the fragility gel after the hydration.
High acyl gellan gum can be brought into play good gel, inborn nature, fixing and conjunctiva effect; High acyl gellan gum has following characteristic simultaneously: be reversible, the hot reversible gel of a kind of shearing; And has fabulous perfume bloom performance; Can be shared with other water-sol, synergy is arranged, splendid figuration effect is arranged with starch, be a kind of elastogel, and can strengthen mouthfeel.Therefore, high acyl gellan gum can be widely used in medicine, chemical industry and the foodstuffs industry, is the favorable substitutes of carrageenin, agar, pectin and other lyophilic colloid, has vast market prospect.
At present, high acyl gellan gum has only one family of U.S. CP Kelco company to produce the domestic high acyl gellan gum of also not accomplishing scale production abroad; The process for extracting of domestic high acyl gellan gum is: carry out salt precipitation earlier; Carry out organic solvent washing again, drying and crushing, this technology can not be removed impurity etc. on the colloid fully; The high acyl gellan gum product purity that makes is low, poorly soluble.The process for extracting of domestic transparent type high acyl gellan gum is like Chinese patent CN101585886A, though prepared high-quality high acyl gellan gum product; But utilize the protease treatment fermented liquid; Complex technical process, production cost is high, can't realize suitability for industrialized production.
Summary of the invention
It is low to the objective of the invention is to solve existing high acyl gellan gum product product purity; Technical problem such as poorly soluble; A kind of high acyl gellan gum production technique is provided; With the fermented liquid heating and filtering, and then carry out organic solvent deposit, solid-liquid separation, decolouring, dehydration, drying, pulverizing and prepare the high acyl gellan gum product.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is following:
High acyl gellan gum production technique of the present invention, this method comprises the steps:
(1) fermented liquid heat filtering: gellan gum fermentation liquid is heated to 80-90 ℃, and adding weight is the flocculating aids of fermented liquid quality 1 ~ 2%, opens and stirs, and behind abundant mixing, pumps into flame filter press and filters;
(2) organic solvent deposit: the glue after will filtering adds the inorganic salt of colloid quality 0.05%-0.1% at temperature 70-80 ℃ of lower alcohol that adds 1.5-3 times of volume down when equitemperature is reduced to below 50 ℃, fully stirring and evenly mixing precipitates colloid fully;
(3) solid-liquid separation: the colloid mixed solution of post precipitation utilizes flame filter press to dewater;
(4) decolouring: the colloid after will dewatering utilizes kibbler to pulverize the lower alcohol that the back adds 1.5-2 times of volume, fully stirs the processing of decolouring in 30-40 minute;
(5) dehydration: the colloid after the decolouring utilizes flame filter press to carry out processed, obtains the wet article of gelling gum;
(6) dry, pulverizing: the wet article of gelling gum are carried out drying, pulverize acquisition high acyl gellan gum finished product.
Step (1) is preferably: gellan gum fermentation liquid is heated to 85 ℃, and adding weight is the flocculating aids of fermented liquid quality 1.5%, and described flocculating aids is perlite or zeyssatite; Plate Filtration pressure is 0.6-1.0Mpa, is preferably 0.8Mpa.
Be preferably in said step (2): the glue after will filtering adds the inorganic salt of colloid quality 0.06% at 75 ℃ of lower alcohols that add 2 times of volumes down of temperature when equitemperature is reduced to below 50 ℃, described lower alcohol is ethanol, Virahol or its combination.Described inorganic salt are sodium-chlor, calcium chloride or magnesium chloride.
In said step (3), Plate Filtration pressure is 0.1-0.4Mpa, is preferably 0.3Mpa.
Step (4) is preferably, and the lower alcohol with the 1.5 times of volumes of colloid adding after pulverizing fully stirs the processing of decolouring in 30 minutes.Said lower alcohol is ethanol, Virahol or its combination.
In said step (5), Plate Filtration pressure is 0.1-0.4Mpa, is preferably 0.3Mpa.
In said step (6), described drying, pulverize and to do, adopt Vacuumdrier or boiling drier, 70 ~ 90 ℃ down oven dry to obtain moisture in 2 ~ 4 hours be the gelling gum 12% below, fully pulverize mixing through pulverizing mill again and obtain the high acyl gellan gum finished product.
The extraction process of high acyl gellan gum of the present invention, technology are simply novel, and production cost is low, and quality product is high, is more suitable in suitability for industrialized production.
Embodiment
Embodiment 1
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste, bean powder or peptone, other be an inorganic salts, fermentation and must fermented liquid.(see reference for details: " Food science " 2008 the 10th phases " the biosynthesizing progress of gelling gum ")
A kind of new high acyl gellan gum production technique of the present invention comprises the steps:
(1) fermented liquid heat filtering
With the 1000Kg fermented liquid is example, at first with the 1000Kg fermented liquid direct heating to 85 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 15Kg; The zeyssatite that in the precoating jar, adds 200Kg deionized water and 10Kg is warming up to 90 ℃, pumps into sheet frame and carries out precoating, preheating, treat filtering after, the fermented liquid after heating is pumped into Plate Filtration, filter pressure is 0.8Mpa.
(2) organic solvent deposit
It is 95% ethanol that glue behind the heat filtering is added the 2000Kg content down 75 ℃ of temperature, adds 0.06% calcium chloride when equitemperature is reduced to below 50 ℃, fully stirs colloid to be precipitated fully in 30 minutes.
(3) solid-liquid separation
Utilize flame filter press to dewater the colloid mixed solution of post precipitation, pressure is 0.3Mpa.
(4) decolouring
Utilizing kibbler to pulverize the back in the colloid after the dehydration, to add 300Kg content be 95% ethanol, fully stirs the processing of decolouring in 30 minutes.
(5) dehydration
Utilize flame filter press to carry out processed in the colloid after the decolouring, obtain the wet article of gelling gum, pressure is 0.3Mpa.
(6) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt Vacuumdrier or boiling drier, under 85 ℃, drying and obtaining moisture in 3 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the high acyl gellan gum finished product more than 95%.
Embodiment 2
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste, bean powder or peptone, other be an inorganic salts, fermentation and must fermented liquid.
A kind of new high acyl gellan gum production technique of the present invention comprises the steps:
(1) fermented liquid heat filtering
With the 1000Kg fermented liquid is example, at first with the 1000Kg fermented liquid direct heating to 88 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 18Kg; The zeyssatite that in the precoating jar, adds 200Kg deionized water and 15Kg is warming up to 85 ℃, pumps into sheet frame and carries out precoating, preheating, treat filtering after, the fermented liquid after heating is pumped into Plate Filtration, filter pressure is 0.6Mpa.
(2) organic solvent deposit
It is 95% ethanol that glue behind the heat filtering is added the 2500Kg content down 70 ℃ of temperature, adds 0.05% calcium chloride when equitemperature is reduced to below 50 ℃, fully stirs colloid to be precipitated fully in 30 minutes.
(3) solid-liquid separation
Utilize flame filter press to dewater the colloid mixed solution of post precipitation, pressure is 0.25Mpa.
(4) decolouring
Utilizing kibbler to pulverize the back in the colloid after the dehydration, to add 400Kg content be 95% ethanol, fully stirs the processing of decolouring in 30 minutes.
(5) dehydration
Utilize flame filter press to carry out processed in the colloid after the decolouring, obtain the wet article of gelling gum, pressure is 0.25Mpa.
(6) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt Vacuumdrier or boiling drier, under 80 ℃, drying and obtaining moisture in 4 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the high acyl gellan gum finished product more than 95%.
Embodiment 3
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste, bean powder or peptone, other be an inorganic salts, fermentation and must fermented liquid.
A kind of new high acyl gellan gum production technique of the present invention comprises the steps:
(1) fermented liquid heat filtering
With the 1000Kg fermented liquid is example, at first with the 1000Kg fermented liquid direct heating to 90 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 20Kg; The zeyssatite that in the precoating jar, adds 200Kg deionized water and 20Kg is warming up to 86 ℃, pumps into sheet frame and carries out precoating, preheating, treat filtering after, the fermented liquid after heating is pumped into Plate Filtration, filter pressure is 1.0Mpa.
(2) organic solvent deposit
It is 95% ethanol that glue behind the heat filtering is added the 3000Kg content down 80 ℃ of temperature, adds 0.08% calcium chloride when equitemperature is reduced to below 50 ℃, fully stirs colloid to be precipitated fully in 30 minutes.
(3) solid-liquid separation
Utilize flame filter press to dewater the colloid mixed solution of post precipitation, pressure is 0.4Mpa.
(4) decolouring
Utilizing kibbler to pulverize the back in the colloid after the dehydration, to add 500Kg content be 95% ethanol, fully stirs the processing of decolouring in 30 minutes.
(5) dehydration
Utilize flame filter press to carry out processed in the colloid after the decolouring, obtain the wet article of gelling gum, pressure is 0.4Mpa.
(6) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt Vacuumdrier or boiling drier, under 88 ℃, drying and obtaining moisture in 2.5 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the high acyl gellan gum finished product more than 95%.
Embodiment 4
Fermented liquid of the present invention be moving slightly Sphingol single-cell under aerobic condition, be carbon source with glucose or sucrose, be nitrogenous source with yeast extract paste, bean powder or peptone, other be an inorganic salts, fermentation and must fermented liquid.
A kind of new high acyl gellan gum production technique of the present invention comprises the steps:
(1) fermented liquid heat filtering
With the 1000Kg fermented liquid is example, at first with the 1000Kg fermented liquid direct heating to 80 of fermentation ends ℃, in fermented liquid, adds the zeyssatite of 13Kg; The zeyssatite that in the precoating jar, adds 200Kg deionized water and 16Kg is warming up to 90 ℃, pumps into sheet frame and carries out precoating, preheating, treat filtering after, the fermented liquid after heating is pumped into Plate Filtration, filter pressure is 0.9Mpa.
(2) organic solvent deposit
It is 95% ethanol that glue behind the heat filtering is added the 1500Kg content down 80 ℃ of temperature, adds 0.1% calcium chloride when equitemperature is reduced to below 50 ℃, fully stirs colloid to be precipitated fully in 30 minutes.
(3) solid-liquid separation
Utilize flame filter press to dewater the colloid mixed solution of post precipitation, pressure is 0.35Mpa.
(4) decolouring
Utilizing kibbler to pulverize the back in the colloid after the dehydration, to add 450Kg content be 95% ethanol, fully stirs the processing of decolouring in 30 minutes.
(5) dehydration
Utilize flame filter press to carry out processed in the colloid after the decolouring, obtain the wet article of gelling gum, pressure is 0.35Mpa.
(6) dry, pulverizing
Through the gelling gum behind the press dewatering, adopt Vacuumdrier or boiling drier, under 92 ℃, drying and obtaining moisture in 2 hours is the gelling gum below 12%; Fully pulverize through pulverizing mill again and mix that to obtain fineness be that 80 order transmitances reach the high acyl gellan gum finished product more than 95%.
Claims (9)
1. a high acyl gellan gum production technique is characterized in that this method comprises the steps:
(1) fermented liquid heat filtering: gellan gum fermentation liquid is heated to 80-90 ℃, and adding weight is the flocculating aids of fermented liquid quality 1 ~ 2%, opens and stirs, and behind abundant mixing, pumps into flame filter press and filters;
(2) organic solvent deposit: the glue after will filtering adds the inorganic salt of colloid quality 0.05%-0.1% at temperature 70-80 ℃ of lower alcohol that adds 1.5-3 times of volume down when equitemperature is reduced to below 50 ℃, fully stirring and evenly mixing precipitates colloid fully;
(3) solid-liquid separation: the colloid mixed solution of post precipitation utilizes flame filter press to dewater;
(4) decolouring: the colloid after will dewatering utilizes kibbler to pulverize the lower alcohol that the back adds 1.5-2 times of volume, fully stirs the processing of decolouring in 30-40 minute;
(5) dehydration: the colloid after the decolouring utilizes flame filter press to carry out processed, obtains the wet article of gelling gum;
(6) dry, pulverizing: the wet article of gelling gum are carried out drying, pulverize acquisition high acyl gellan gum finished product.
2. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (1), described flocculating aids is perlite or zeyssatite.
3. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (1), Plate Filtration pressure is 0.6-1.0Mpa.
4. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (2), described lower alcohol is ethanol, Virahol or its combination.
5. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (2), described inorganic salt are sodium-chlor, calcium chloride or magnesium chloride.
6. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (3), Plate Filtration pressure is 0.1-0.4Mpa.
7. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (4), described lower alcohol is ethanol, Virahol or its combination.
8. high acyl gellan gum production technique according to claim 1 is characterized in that: in said step (5), Plate Filtration pressure is 0.1-0.4Mpa.
9. high acyl gellan gum production technique according to claim 1; It is characterized in that; In said step (6), described drying, pulverizing are to adopt Vacuumdrier or boiling drier; Under 70 ~ 90 ℃, drying and obtaining moisture in 2 ~ 4 hours is the gelling gum below 12%, fully pulverizes to mix through pulverizing mill again to obtain the high acyl gellan gum finished product.
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| CN2011102973045A CN102391384A (en) | 2011-09-30 | 2011-09-30 | Production process for high acyl gellan gum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627699A (en) * | 2012-03-30 | 2012-08-08 | 杭州健恒生物技术有限公司 | Extraction method for high acyl gellan gum |
| CN103509845A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | High aryl gellan gum extracting method |
| WO2016167939A1 (en) * | 2015-04-13 | 2016-10-20 | Cp Kelco U.S., Inc. | Gellan gum products and methods of manufacture and use thereof |
| CN108559762A (en) * | 2018-03-12 | 2018-09-21 | 苏州富博宏新材料科技有限公司 | A kind of clean preparation method of gellan gum |
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1687437A (en) * | 2005-03-25 | 2005-10-26 | 张禹 | Method of high post extraction of acyl Gellan |
| CN101191138A (en) * | 2006-11-28 | 2008-06-04 | 尹洪波 | Method for producing gellan gum finished product from gellan gum fermentation liquor |
| CN101591400A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of method for post extraction of low-acyl clean-type gellan gum |
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2011
- 2011-09-30 CN CN2011102973045A patent/CN102391384A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687437A (en) * | 2005-03-25 | 2005-10-26 | 张禹 | Method of high post extraction of acyl Gellan |
| CN101191138A (en) * | 2006-11-28 | 2008-06-04 | 尹洪波 | Method for producing gellan gum finished product from gellan gum fermentation liquor |
| CN101591400A (en) * | 2009-07-09 | 2009-12-02 | 浙江中肯生物科技有限公司 | A kind of method for post extraction of low-acyl clean-type gellan gum |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627699A (en) * | 2012-03-30 | 2012-08-08 | 杭州健恒生物技术有限公司 | Extraction method for high acyl gellan gum |
| CN103509845A (en) * | 2013-08-09 | 2014-01-15 | 新疆阜丰生物科技有限公司 | High aryl gellan gum extracting method |
| WO2016167939A1 (en) * | 2015-04-13 | 2016-10-20 | Cp Kelco U.S., Inc. | Gellan gum products and methods of manufacture and use thereof |
| US10638783B2 (en) | 2015-04-13 | 2020-05-05 | Cp Kelco U.S., Inc. | Gellan gum products and methods of manufacture and use thereof |
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
| CN108559762A (en) * | 2018-03-12 | 2018-09-21 | 苏州富博宏新材料科技有限公司 | A kind of clean preparation method of gellan gum |
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Application publication date: 20120328 |