CN102390889A - Method for treating pulping and papermaking wastewater by using dominant degradation bacteria - Google Patents
Method for treating pulping and papermaking wastewater by using dominant degradation bacteria Download PDFInfo
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Abstract
The invention discloses a method for treating pulping and papermaking wastewater by using dominant degradation bacteria. The method comprises the following steps of: screening out four dominant species having the optimal effect of degrading the chemical oxygen demand of the pulping and papermaking wastewater from six types of bacteria by using a statistical analysis method, wherein the four dominant species are agrobactium tumefaciens, bacillus, Gordonia and pseudomonas putida; performing oscillating culture for 36 hours; heating and dissolving 1.5 grams of sodium alginate, adding bacterium suspension respectively, and injecting into a calcium chloride aqueous solution by using an injector respectively to prepare cured gel particles; and adding 10 to 12 percent of agrobactium tumefaciens cured gel particles, 23 to 25 percent of bacillus cured gel particles, 31 to 35 percent of Gordonia cured gel particles and 28 to 36 percent of pseudomonas putida cured gel particles into the pulping and papermaking wastewater according to volume percentage, and detecting a chemical oxygen demand change situation and a chroma change situation of the pulping and papermaking wastewater.
Description
Technical field
The present invention relates to the pulp and paper effluent treatment field, specifically is a kind of method of utilizing the advantage degradation bacteria to handle paper-making effluent.
Background technology
The statistics experimental design can be analyzed the interaction of multiple microbial population, can also improve processing efficiency through the formation of optimizing nutrient solution.Therefore, the statistical experiment design has been widely used in every field, as optimizing substratum and condition in the biological chemistry; Extract trace element and measure benzene; Toluene, ethylbenzene and YLENE material, yet; As far as our knowledge goes, according to statistical report the fewer of the effect of associate strain on pulp and paper effluent treatment is described.Therefore, the interaction of research various bacteria combination becomes an important factor of administering paper waste.Traditional method is ignored complex interactions between the various mikrobes.On the contrary, the statistical experiment design meets this requirement especially like factor design and response surface analysis.
Paper-making effluent concentration is high, and COD, BOD content are big, and its treatment process is different than commonly industrial wastewater, and at present, the technological process of treating papermaking method mainly contains physics method, chemical method, biological process and physico-chemical processes.Wherein the application of biological process is the most extensive, has become one of main method of paper waste second-stage treatment.The biologic treating technique of waste water is exactly the metabolic function that utilizes mikrobe, makes to be dissolving and the organic pollutant of colloidal state in the waste water and to be degraded and to be converted into harmless stable material, thereby waste water is able to only
Change.Biological treatment is to remove BOD, the indispensable two stage biological treating processes of COD, and it has effects such as removing SS, decolouring, deodorizing concurrently.According to the microbe species and the oxygen supply situation of participation effect, be divided into aerobe processing, anaerobic biological treatment and aerobic-anaerobic combined treatment three major types.But this mikrobe directly acts on the technological process of treating papermaking method and has satisfied not the requirements of people to waste water treatment, so under the support of new biotechnology, the biological reinforcing technology of wastewater treatment arises at the historic moment.
Immobilized microorganism technique is an emerging technology in the modern biological project field, is in the area of space that free cell or enzyme are positioned to limit of the means through chemistry or physics, makes it keep method active and that can recycle.
Summary of the invention
The objective of the invention is to deficiency, a kind of method of utilizing the advantage degradation bacteria to handle paper-making effluent is provided to prior art.
The object of the invention is realized through following technical scheme:
A kind of method of utilizing the advantage degradation bacteria to handle paper-making effluent comprises the steps:
(1) get 1 ~ 2 the ring edaphic bacillus (
Agrobacterium sp.), rod bacterium (
Bacillus sp.), enterobacteria (
Enterobacter. Cloacae.), Gordon Salmonella (
Gordonia sp.), pseudomonas putida (
Pseudomonas putida.), Pseudomonas stutzeri (
Pseudomonas stutzeri.) insert respectively in the nutritional medium, after cultivating 36h under 30 ℃, take out, and with whizzer centrifugal 5min under 10000rpm speed, once centrifugal with similarity condition again after taking-up deposition and the washing of adding phosphoric acid buffer, collect six kinds of thalline that obtain;
(2) utilizing the statistical analysis method to filter out 4 kinds above-mentioned six kinds of bacterium has the dominant strain of optimum degradation effect to paper-making effluent COD degraded, is respectively: edaphic bacillus, rod bacterium, Gordon Salmonella, pseudomonas putida;
(3) above-mentioned four kinds of dominant bacterias that filter out are inserted in the substratum; Shaking culture 36 h under 30 ℃, 150 r/min, the nutrient solution that will comprise thalline is poured into respectively in the centrifuge tube, centrifugal 10min under 5000r/min; And with phosphoric acid buffer washing 2 times; Abandon supernatant, take out deposition and be made into bacteria suspension, refrigerate subsequent use with 10 ml inorganic salt solutions;
(4) adding of 1.5g sodium-alginate is equipped with in the triangular flask of 50ml zero(ppm) water; Heating for dissolving; Under 121 ℃ of conditions the sterilization 15min after; Be cooled to 60 ℃, add edaphic bacillus bacteria suspension, rod bacterium bacteria suspension, Gordon Salmonella bacteria suspension and pseudomonas putida bacteria suspension respectively, the sodium alginate soln that will insert thalline then use syringe respectively implantation quality per-cent be in 2% the calcium chloride water; Be prepared into the immobilization gel particle of 2 ~ 3mm; Put into refrigerator then and solidify 4h, with immobilization gel particle washing 3 times, prepare edaphic bacillus immobilization particle, rod bacterium immobilization particle, Gordon Salmonella immobilization particle and pseudomonas putida immobilization particle respectively with sterilized water;
(5) four kinds of immobilization gel-based particles with above-mentioned preparation take out; Per-cent meter by volume; Get 10～12% edaphic bacillus immobilization gel-based particles, 23～25% rod bacterium immobilization gel-based particles, 31～35% Gordon Salmonella immobilization gel-based particles and 28～36% pseudomonas putida immobilization gel-based particles respectively; 20 ml altogether; Join in the conical flask that contains 200 ml paper-making effluents, shaking culture under 30 ℃, 150 r/min detects its COD and changes and the colourity changing conditions.
Six kinds of different bacteriums are adopted different nutritional mediums in the said step (1), and wherein the nutritional medium of edaphic bacillus, rod bacterium and enterobacteria is Carnis Bovis seu Bubali cream 3.0g/L, Tryptones 5.0g/L; Gordon Salmonella nutritional medium is glucose 10.0g/L, yeast powder 10.0g/L; The pseudomonas putida nutritional medium is Carnis Bovis seu Bubali cream 1.5g/L, glucose 1.0g/L, Tryptones 6.0 g/L, yeast powder 3.0g/L; The nutritional medium of Pseudomonas stutzeri is casein 17.0g/L, potassium hydrogen phosphate 2.5 g/L, glucose 2.5 g/L, soyflour 3.0 g/L, sodium-chlor 5.0g/L.
Said paper-making effluent refers to mixed by sulphate process bamboo pulp waste wash liquor and dioxide peroxide, chelating, hydrogen peroxide tri-stage bleaching waste water, and its COD is 1325 mg/L.
Compared with prior art; The present invention has following advantage and beneficial effect: the present invention adopts statistical experimental design method to filter out the dominant strain of the optimum degradation effect of paper-making effluent; Respectively after the immobilization, form microorganism species with sodium-alginate by a certain percentage, this technology has the biological density height, is swift in response, biological number of dropouts is few, reaction control is easy to advantage; This technology is applied to pulp and paper effluent treatment; Help improving the microbe density in the bio-reactor, be beneficial to reacted solid-liquid separation, shorten and handle the required time.
Embodiment
Below in conjunction with specific embodiment the present invention is done further concrete detailed description the in detail, but embodiment of the present invention is not limited thereto, the processing parameter for not indicating especially can carry out with reference to routine techniques.
Embodiment 1
(1) get 1 the ring edaphic bacillus (
Agrobacterium sp.), rod bacterium (
Bacillus sp.), enterobacteria (
Enterobacter. Cloacae.), Gordon Salmonella (
Gordonia sp.), pseudomonas putida (
Pseudomonas putida.), Pseudomonas stutzeri (
Pseudomonas stutzeri.) insert respectively in the nutritional medium, after cultivating 36h under 30 ℃, take out, and with whizzer centrifugal 5min under 10000rpm speed, once centrifugal with similarity condition again after taking-up deposition and the washing of adding phosphoric acid buffer, collect six kinds of thalline that obtain;
(2) utilizing the statistical analysis method to filter out 4 kinds above-mentioned six kinds of bacterium has the dominant strain of optimum degradation effect to paper-making effluent COD degraded, is respectively: edaphic bacillus, rod bacterium, Gordon Salmonella, pseudomonas putida;
(3) above-mentioned four kinds of dominant bacterias that filter out are inserted in the substratum; Shaking culture 36 h under 30 ℃, 150 r/min, the nutrient solution that will comprise thalline is poured into respectively in the centrifuge tube, centrifugal 10min under 5000r/min; And with phosphoric acid buffer washing 2 times; Abandon supernatant, take out deposition and be made into bacteria suspension, refrigerate subsequent use with 10 ml inorganic salt solutions;
(4) adding of 1.5g sodium-alginate is equipped with in the triangular flask of 50ml zero(ppm) water, heating for dissolving is behind the 15min that sterilizes under 121 ℃ of conditions; When being cooled to 60 ℃; Add the edaphic bacillus bacteria suspension, the sodium alginate soln use syringe implantation quality per-cent that will insert thalline then is in 2% the calcium chloride water, is prepared into the immobilization gel particle of 2 ~ 3mm; Put into refrigerator then and solidify 4h; With immobilization gel particle washing 3 times, preparation edaphic bacillus immobilization particle uses the same method and processes rod bacterium, Gordon Salmonella, pseudomonas putida immobilization particle respectively with sterilized water;
(5) the immobilization gel-based particle with above-mentioned preparation takes out; Per-cent meter by volume; Get 11% edaphic bacillus, 23% rod bacterium, 33% Gordon Salmonella, 33% pseudomonas putida totally 20 ml respectively; Join in the conical flask that contains 200 ml paper-making effluents, shaking culture under 30 ℃, 150 r/min detects its COD and colourity changing conditions.
The Eucalyptus NBSK pulp-making waste-water that adopts the present embodiment method to handle, the clearance of handling its final COD variation of back and colourity through 12 hours reaches 75% and 56%, is higher than the control group 70% and 21% that does not add dominant microflora.
Embodiment 2
(1) get 2 the ring edaphic bacilluss (
Agrobacterium sp.), rod bacterium (
Bacillus sp.), enterobacteria (
Enterobacter. Cloacae.), Gordon Salmonella (
Gordonia sp.), pseudomonas putida (
Pseudomonas putida.), Pseudomonas stutzeri (
Pseudomonas stutzeri.) insert respectively in the nutritional medium, after cultivating 36h under 30 ℃, take out, and with whizzer centrifugal 5min under 10000rpm speed, once centrifugal with similarity condition again after taking-up deposition and the washing of adding phosphoric acid buffer, collect six kinds of thalline that obtain;
(2) utilizing the statistical analysis method to filter out 4 kinds above-mentioned six kinds of bacterium has the dominant strain of optimum degradation effect to paper-making effluent COD degraded, is respectively: edaphic bacillus, rod bacterium, Gordon Salmonella, pseudomonas putida;
(3) above-mentioned four kinds of dominant bacterias that filter out are inserted in the substratum; Shaking culture 36 h under 30 ℃, 150 r/min, the nutrient solution that will comprise thalline is poured into respectively in the centrifuge tube, centrifugal 10min under 5000r/min; And with phosphoric acid buffer washing 2 times; Abandon supernatant, take out deposition and be made into bacteria suspension, refrigerate subsequent use with 10 ml inorganic salt solutions;
(4) adding of 1.5g sodium-alginate is equipped with in the triangular flask of 50ml zero(ppm) water, heating for dissolving is behind the 15min that sterilizes under 121 ℃ of conditions; When being cooled to 60 ℃; Add the edaphic bacillus bacteria suspension, the sodium alginate soln use syringe implantation quality per-cent that will insert thalline then is in 2% the calcium chloride water, is prepared into the immobilization gel particle of 2 ~ 3mm; Put into refrigerator then and solidify 4h; With immobilization gel particle washing 3 times, preparation edaphic bacillus immobilization particle uses the same method and processes rod bacterium, Gordon Salmonella, pseudomonas putida immobilization particle respectively with sterilized water;
(5) the immobilization gel-based particle with above-mentioned preparation takes out; Per-cent meter by volume; Get 10% edaphic bacillus, 24% rod bacterium, 32% Gordon Salmonella, 34% pseudomonas putida totally 20 ml respectively; Join in the conical flask that contains 200 ml paper-making effluents, shaking culture under 30 ℃, 150 r/min detects its COD and colourity changing conditions.
The Eucalyptus NBSK pulp-making waste-water that adopts the present embodiment method to handle, the clearance of handling its final COD variation of back and colourity through 12 hours reaches 73% and 52%, is higher than the control group 69% and 24% that does not add dominant microflora.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1. a method of utilizing the advantage degradation bacteria to handle paper-making effluent is characterized in that, comprises the steps:
(1) get 1 ~ 2 the ring edaphic bacillus (
Agrobacterium sp.), rod bacterium (
Bacillus sp.), enterobacteria (
Enterobacter. Cloacae.), Gordon Salmonella (
Gordonia sp.), pseudomonas putida (
Pseudomonas putida.), Pseudomonas stutzeri (
Pseudomonas stutzeri.) insert respectively in the nutritional medium, after cultivating 36h under 30 ℃, take out, and with whizzer centrifugal 5min under 10000rpm speed, once centrifugal with similarity condition again after taking-up deposition and the washing of adding phosphoric acid buffer, collect six kinds of thalline that obtain;
(2) utilizing the statistical analysis method to filter out 4 kinds above-mentioned six kinds of bacterium has the dominant strain of optimum degradation effect to paper-making effluent COD degraded, is respectively: edaphic bacillus, rod bacterium, Gordon Salmonella, pseudomonas putida;
(3) above-mentioned four kinds of dominant bacterias that filter out are inserted in the substratum; Shaking culture 36 h under 30 ℃, 150 r/min, the nutrient solution that will comprise thalline is poured into respectively in the centrifuge tube, centrifugal 10min under 5000r/min; And with phosphoric acid buffer washing 2 times; Abandon supernatant, take out deposition and be made into bacteria suspension, refrigerate subsequent use with 10 ml inorganic salt solutions;
(4) adding of 1.5g sodium-alginate is equipped with in the triangular flask of 50ml zero(ppm) water; Heating for dissolving; Under 121 ℃ of conditions the sterilization 15min after; Be cooled to 60 ℃, add edaphic bacillus bacteria suspension, rod bacterium bacteria suspension, Gordon Salmonella bacteria suspension and pseudomonas putida bacteria suspension respectively, the sodium alginate soln that will insert thalline then use syringe respectively implantation quality per-cent be in 2% the calcium chloride water; Be prepared into the immobilization gel particle of 2 ~ 3mm; Put into refrigerator then and solidify 4h, with immobilization gel particle washing 3 times, prepare edaphic bacillus immobilization particle, rod bacterium immobilization particle, Gordon Salmonella immobilization particle and pseudomonas putida immobilization particle respectively with sterilized water;
(5) four kinds of immobilization gel-based particles with above-mentioned preparation take out; Per-cent meter by volume; Get 10～12% edaphic bacillus immobilization gel-based particles, 23～25% rod bacterium immobilization gel-based particles, 31～35% Gordon Salmonella immobilization gel-based particles and 28～36% pseudomonas putida immobilization gel-based particles respectively; 20 ml altogether; Join in the conical flask that contains 200 ml paper-making effluents, shaking culture under 30 ℃, 150 r/min detects its COD and changes and the colourity changing conditions.
2. preparation method according to claim 1; It is characterized in that; Six kinds of different bacteriums are adopted different nutritional mediums in the said step (1), and wherein the nutritional medium of edaphic bacillus, rod bacterium and enterobacteria is Carnis Bovis seu Bubali cream 3.0g/L, Tryptones 5.0g/L; Gordon Salmonella nutritional medium is glucose 10.0g/L, yeast powder 10.0g/L; The pseudomonas putida nutritional medium is Carnis Bovis seu Bubali cream 1.5g/L, glucose 1.0g/L, Tryptones 6.0 g/L, yeast powder 3.0g/L; The nutritional medium of Pseudomonas stutzeri is casein 17.0g/L, potassium hydrogen phosphate 2.5 g/L, glucose 2.5 g/L, soyflour 3.0 g/L, sodium-chlor 5.0g/L.
3. preparation method according to claim 1 is characterized in that, said paper-making effluent refers to mixed by sulphate process bamboo pulp waste wash liquor and dioxide peroxide, chelating, hydrogen peroxide tri-stage bleaching waste water, and its COD is 1325 mg/L.
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| CN103087966A (en) * | 2013-02-19 | 2013-05-08 | 广州市微生物研究所 | Efficient degrading microbial inoculum for regenerated papermaking wastewater and preparation method thereof |
| CN103469663A (en) * | 2013-08-13 | 2013-12-25 | 广西大学 | Method for optimization of paper pulp chlorine dioxide bleaching by response surface methodology |
| CN103627657A (en) * | 2013-11-11 | 2014-03-12 | 华南理工大学 | Composite microflora for degrading lignin wastewater and preparation method thereof |
| CN103642712A (en) * | 2013-11-08 | 2014-03-19 | 华南理工大学 | Composite flora for degrading papermaking waste water and preparation method thereof |
| CN103667112A (en) * | 2013-11-13 | 2014-03-26 | 华南理工大学 | Complex bacterium for degrading electroplating wastewater and preparation method of complex bacterium |
| CN107244781A (en) * | 2017-06-27 | 2017-10-13 | 安徽比伦生活用纸有限公司 | A kind of processing method of paper waste |
| CN107613774A (en) * | 2014-11-25 | 2018-01-19 | 科罗拉多州立大学研究基金会 | For mobilizing the collaboration bacterial consortium of soil phophorus |
| CN110194534A (en) * | 2019-06-14 | 2019-09-03 | 湖南工学院 | The preparation method of the multifunctional recycled material of formaldehyde in a kind of degrading waste water |
| CN113845236A (en) * | 2021-11-15 | 2021-12-28 | 南京环保产业创新中心有限公司 | Immobilized filler, preparation method thereof, and photosynthetic bacteria-protozoan sewage treatment device and method based on filler |
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2011
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| CN103087966B (en) * | 2013-02-19 | 2016-02-10 | 广州市微生物研究所 | A kind of regeneration and reparation high efficiency degradation bacterial agent and preparation method thereof |
| CN103087966A (en) * | 2013-02-19 | 2013-05-08 | 广州市微生物研究所 | Efficient degrading microbial inoculum for regenerated papermaking wastewater and preparation method thereof |
| CN103469663A (en) * | 2013-08-13 | 2013-12-25 | 广西大学 | Method for optimization of paper pulp chlorine dioxide bleaching by response surface methodology |
| CN103469663B (en) * | 2013-08-13 | 2016-05-04 | 广西大学 | A kind of by response surface method optimization paper pulp ClO 2 bleaching method |
| CN103642712A (en) * | 2013-11-08 | 2014-03-19 | 华南理工大学 | Composite flora for degrading papermaking waste water and preparation method thereof |
| CN103627657B (en) * | 2013-11-11 | 2016-01-06 | 华南理工大学 | A kind of composite flora for lignin degrading waste water and preparation method thereof |
| CN103627657A (en) * | 2013-11-11 | 2014-03-12 | 华南理工大学 | Composite microflora for degrading lignin wastewater and preparation method thereof |
| CN103667112A (en) * | 2013-11-13 | 2014-03-26 | 华南理工大学 | Complex bacterium for degrading electroplating wastewater and preparation method of complex bacterium |
| CN107613774A (en) * | 2014-11-25 | 2018-01-19 | 科罗拉多州立大学研究基金会 | For mobilizing the collaboration bacterial consortium of soil phophorus |
| CN107244781A (en) * | 2017-06-27 | 2017-10-13 | 安徽比伦生活用纸有限公司 | A kind of processing method of paper waste |
| CN110194534A (en) * | 2019-06-14 | 2019-09-03 | 湖南工学院 | The preparation method of the multifunctional recycled material of formaldehyde in a kind of degrading waste water |
| CN113845236A (en) * | 2021-11-15 | 2021-12-28 | 南京环保产业创新中心有限公司 | Immobilized filler, preparation method thereof, and photosynthetic bacteria-protozoan sewage treatment device and method based on filler |
| CN113845236B (en) * | 2021-11-15 | 2023-07-21 | 南京环保产业创新中心有限公司 | Immobilized filler, preparation method thereof, photosynthetic bacteria-protozoan sewage treatment device and method based on immobilized filler |
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Application publication date: 20120328 |