CN102367477A - 用饱和染料进行核酸解链分析 - Google Patents
用饱和染料进行核酸解链分析 Download PDFInfo
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- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
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Abstract
Description
染料 | Ex/Em1 | 最大PCR相容的%Sat2 | %Het3 |
G5 | 442-458/475 | 100% | 20.0% |
H5 | 444/459 | 100% | 22.5% |
S5 | 450/469 | >99% | 20.5% |
D6 | 457/471 | 92% | 23.3% |
E6 | 425/454 | >99% | 15.0% |
P6 | 464/490 | 100% | 21.0% |
R6 | 453/470 | >90% | 15.0% |
Y6 | 439/477-515 | 100% | 21.0% |
Z6 | 469/494-526 | 100% | 13.4% |
N7 | 458/474 | >100% | 22.0% |
O7 | **4 | >70% | 21.2% |
P7 | **4 | >70% | 21.5% |
Q7 | 453/471 | >70% | 20.7% |
T7 | 453/471 | >70% | 21.2% |
G8 | 453/471 | >100% | 19.7% |
L8 | 470-490/490-520 | >70% | 2.3% |
P8 | 453/471 | >70% | 17.7% |
T8 | 453/471 | >70% | 24.0% |
V8 | 469/494-526 | >70% | 21.4% |
W8 | 453/471 | >70% | 27.5% |
Z8 | 453/471 | >70% | 22.7% |
A9 | **4 | 100% | 23.0% |
I9 | **4 | 100% | 20.9% |
I9Met | **4 | >80% | 23.0% |
J9 | **4 | 100% | 22.4% |
J9Met | **4 | 100% | 23.0% |
K9 | **4 | 100% | 22.3% |
L9 | **4 | 100% | 21.3% |
V10 | **4 | 95% | 15.5% |
F11 | **4 | 100% | 22.0% |
H11 | **4 | 100% | 13.0% |
突变 | 核苷酸位置 | 氨基酸改变 | 外显子 |
I506V | 在1648上是A或G | 在506上为Ile或Val | 10 |
I507del | 在1648-1653间缺失3bp | 缺失Ile506或Ile507 | 10 |
F508del | 在1652-1655间缺失3bp | 在508上缺失Phe | 10 |
F508C | 在1655上是T或G | 在508上为Phe或Cys | 10 |
G542X | 在1756上由G至T | 在542上由Gly变为终止密码子 | 11 |
引物 | 序列(5’→3’) | SEQ.ID.NO. |
外显子9正向 | GATGCTCTGCTTCTGTACTG | SEQ ID NO.40 |
外显子9反向 | GCCTAAACATCCCCTTAAATTGG | SEQ ID NO.41 |
外显子11正向 | CTCTCCAGAGTGCTCTAATGAC | SEQ ID NO.42 |
外显子11反向 | AGCCCCTGTTTCATACTGACC | SEQ ID NO.43 |
外显子13正向 | CGGCCATGACTGTCGCTGTAA | SEQ ID NO.44 |
外显子13反向 | CTCCAATGGTGCAGGCTCCAA | SEQ ID NO.45 |
外显子17正向 | TCTCCTCCAACCTAATAGTG | SEQ ID NO.46 |
外显子17反向 | GGACTGTCAAGCAGAGAAT | SEQ ID NO.47 |
Claims (29)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/827,890 US7387887B2 (en) | 2004-04-20 | 2004-04-20 | Nucleic acid melting analysis with saturation dyes |
US10/827,890 | 2004-04-20 |
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CN2005800197042A Division CN101198706B (zh) | 2004-04-20 | 2005-04-20 | 用饱和染料进行核酸解链分析 |
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CN102367477A true CN102367477A (zh) | 2012-03-07 |
CN102367477B CN102367477B (zh) | 2015-04-15 |
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CN201110321820.7A Active CN102367477B (zh) | 2004-04-20 | 2005-04-20 | 用饱和染料进行核酸解链分析 |
CN2005800197042A Active CN101198706B (zh) | 2004-04-20 | 2005-04-20 | 用饱和染料进行核酸解链分析 |
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CN2005800197042A Active CN101198706B (zh) | 2004-04-20 | 2005-04-20 | 用饱和染料进行核酸解链分析 |
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US (2) | US7387887B2 (zh) |
EP (3) | EP2574678B1 (zh) |
JP (1) | JP5507047B2 (zh) |
CN (2) | CN102367477B (zh) |
AU (2) | AU2005329731B8 (zh) |
CA (2) | CA2562780C (zh) |
ES (2) | ES2525673T3 (zh) |
WO (1) | WO2006121423A2 (zh) |
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CN101198706B (zh) | 2011-10-26 |
WO2006121423A3 (en) | 2007-11-22 |
CA2562780C (en) | 2013-02-19 |
WO2006121423A2 (en) | 2006-11-16 |
EP2574678A1 (en) | 2013-04-03 |
AU2005329731B8 (en) | 2011-02-10 |
CN102367477B (zh) | 2015-04-15 |
EP1774047A2 (en) | 2007-04-18 |
CN101198706A (zh) | 2008-06-11 |
AU2011202040B2 (en) | 2012-07-26 |
EP2826867B1 (en) | 2020-06-03 |
JP5507047B2 (ja) | 2014-05-28 |
ES2527446T3 (es) | 2015-01-23 |
US7387887B2 (en) | 2008-06-17 |
AU2005329731B2 (en) | 2011-02-03 |
EP1774047A4 (en) | 2010-03-03 |
AU2005329731A1 (en) | 2006-11-09 |
ES2525673T3 (es) | 2014-12-29 |
AU2011202040A1 (en) | 2011-05-26 |
EP2574678B1 (en) | 2014-09-10 |
CA2795339A1 (en) | 2005-10-20 |
EP2826867A1 (en) | 2015-01-21 |
EP1774047B1 (en) | 2014-10-08 |
US20090117553A1 (en) | 2009-05-07 |
CA2795339C (en) | 2018-12-04 |
AU2005329731A8 (en) | 2008-07-31 |
JP2008510698A (ja) | 2008-04-10 |
US20050233335A1 (en) | 2005-10-20 |
CA2562780A1 (en) | 2005-10-20 |
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