CN102357134B - Medicine for preventing and treating Alzheimer disease and cerebral thrombosis and preparation method thereof - Google Patents
Medicine for preventing and treating Alzheimer disease and cerebral thrombosis and preparation method thereof Download PDFInfo
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Abstract
The invention relates to Chinese medicinal herb, in particular to a medicine for preventing and treating Alzheimer disease and cerebral thrombosis and a preparation method thereof, which belong to the field of medicines. The preparation mainly comprises the following raw materials by weight: 30-120 parts of pseudo-ginseng, 30-120 parts of Ligusticum wallichii, 30-120 parts of safflower carthamus, 30-120 parts of peach kernel, 90-360 parts of kudzuvine root, 50-150 parts of total flavones and 50-150 parts of Gypenoside. The preparation mainly used for preventing and treating the Alzheimer disease and the cerebral thrombosis.
Description
Technical field:
The present invention relates to a kind ofly with Chinese herbal medicine, definitely say medicine for prevention and treatment Alzheimer and cerebral thrombosis and preparation method thereof, belong to drug world.
Background technology:
Alzheimer (AD), claim again alzheimer disease, a kind of to hide onset and the deterioration of carrying out property or persistence hypophrenia as the neurodegenerative disease of feature, the category that belongs to organic mental sickness, can seriously reduce Quality of Life, cause serious financial burden and stress for family and society.Many diseases of AD are in the geratic period, and every increase of age is 5 years old after 65 years old, and prevalence doubles.Along with the fast development of social population's aging, the proportion of old people in the crowd is increasing, and AD will become a very important health and social problem.
The AD pathogenesis is complicated, and the medicine that has gone on the market at present mainly is divided into two kinds, and a kind of is cholinesterase inhibitor, and example hydrochloric acid donepezil, galantamine, rivastigmine, huperzine are first-class; Another kind is excitatory amino acid receptor antagonists, the example hydrochloric acid memantine.Other has antiinflammatory, antioxidation, anti-cholesterol, directed against amyloid-beta protein medicaments to be used for the auxiliary treatment of AD.Said medicine can short-term be improved AD patient's symptom, but can not alleviate advancing of disease.Chinese medicine begins to be subject to the concern of Chinese scholars in recent years because of many target spots, too many levels and multimode mechanism of action.
Senile dementia belongs to the categories such as Chinese medicine " dementia " " forgetful ", how because of the imbalance of the liver functions such as vital essence loss, YANG-orifices failing to be nourished or the heart, liver, spleen, causes gas, blood, expectorant, fire, all evil retardance brain keys of the stasis of blood and forms.The patient clinical main manifestations is for slow-witted, stupid, stupid, stupid, and the Therapeutic Principle mainly contains tonify deficiency righting, blood circulation promoting and blood stasis dispelling etc.
Cerebral thrombosis is brain under slow blood flow, condition that blood pressure is on the low side, and blood formed element is attached to the interior film formed thrombosis of tremulous pulse, is more common in middle-aged and elderly people.Cerebral thrombosis claims again cerebral infarction, " apoplexy " category that belongs to Chinese medicine, how form because of gas, blood, wind, the stagnation of phlegm meridians that stagnate, patient's onset is slower, clinical manifestation is the obstacles such as language, motor function, psychology and emotion in various degree, brings heavy financial burden and stress equally for family and society with AD patient.The Therapeutic Principle is mainly blood circulation promoting and blood stasis dispelling, promoting of the circulation of QI and removing the obstruction in the collaterals, suppressing the hyperactive liver and reduces phlegm etc.
Summary of the invention:
The purpose of this invention is to provide medicine for prevention and treatment Alzheimer and cerebral thrombosis and preparation method thereof, the present invention has the increase cerebral blood flow, improves microcirculation, strengthens the tolerance to cerebral ischemia, improves antioxidation system; Reduce whole blood viscosity and plasma viscosity, the effects such as anticoagulant and thrombosis.
Technical scheme of the present invention, for the medicine of prevention and treatment Alzheimer and cerebral thrombosis, it is characterized in that: its raw material mainly is comprised of the following weight ratio:
Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part.
Its raw material is comprised of the following weight ratio:
49 parts of Radix Notoginseng, 49 parts of Rhizoma Chuanxiongs, 57.5 parts on Flos Carthami, 57.5 parts in Semen Persicae, 164 parts of Radix Puerariaes.
Its raw material is comprised of the following weight ratio:
Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part, Fructus Crataegi flavone 50-150 part, gypenoside 50-150 part.
Its raw material weight ratio is:
49 parts of Radix Notoginseng, 49 parts of Rhizoma Chuanxiongs, 57.5 parts on Flos Carthami, 57.5 parts in Semen Persicae, 164 parts of Radix Puerariaes, 90 parts of Fructus Crataegi flavone, 90 parts of gypenosides.
Its described medicine is used for prevention and treatment Alzheimer and cerebral thrombosis.
Be used for the preparation method of the medicine of prevention and treatment Alzheimer and cerebral thrombosis, it is characterized in that: get crude drug by weight proportion: Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part, medical material is made only fragmentation;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, and makes medicine by the industry routine.
The preparation method that is used for the medicine of prevention and treatment Alzheimer and cerebral thrombosis, it is characterized in that: get crude drug by weight proportion: Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part, Fructus Crataegi flavone 50-150 part, gypenoside 50-150 part; Medical material is made only fragmentation;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, with Fructus Crataegi flavone, gypenoside mix homogeneously according to the above ratio, make medicine by the industry routine.
Wherein said medicine is tablet, capsule, granule.
Characteristics of the present invention:
Theory of Chinese medical science:
The present invention be at distinguished veteran doctors of TCM on the basis of proved recipe, test resulting preparation through a large amount of preparation technologies.Medicine of the present invention is mainly the extract of Radix Puerariae, Radix Notoginseng, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami.Wherein Radix Puerariae is hot, sweet, cool, returns spleen, stomach warp.Xin Zeneng is loose, and the tonification of sweet energy is so can mend precise and tiny by qi and blood circulation promotion; Radix Notoginseng sweet in the mouth, little hardship are returned liver, stomach warp, but blood stasis dispelling and just not hindering.Two medicines share, and the merit that qi and blood circulation promotion, blood stasis dispelling stagnate is better, is monarch drug altogether.The hot temperature rise of Rhizoma Chuanxiong is fallen apart, is " gas medicine in the blood ", and top, the sensible top of the up head of energy, the function blood-activating and qi-promoting, wind-expelling pain-stopping helps Radix Puerariae, pseudo-ginseng blood-circulation-invigovating regulating qi to disperse stagnation, removing obstruction in the collateral to relieve pain, and the up arrival brain of energy priming, is ministerial drug.Semen Persicae, Flos Carthami help Radix Puerariae, Radix Notoginseng, Rhizoma Chuanxiong blood circulation promoting and blood stasis dispelling, and removing obstruction in the collateral to relieve pain is adjuvant drug.
Another characteristics of the present invention are on the said medicine basis, increase the higher natural drug extract of purity, play the effect that strengthens effect of drugs.Wherein nourish heart spleen invigorating, QI invigorating and blood regulating, expectorant blood stasis dispelling, blood fat reducing of gypenoside cooperates the Radix Puerariae yang invigorating, plays simultaneously the effect of righting; Fructus Crataegi flavone is returned spleen, stomach, Liver Channel, stomach invigorating circulation of qi promoting dissipating blood stasis.Full side share, promoting flow of QI and blood, and disperse blood stasis and dredge collateral, strengthening vital QI to eliminate pathogenic factors is applicable to prevention and the treatment of Alzheimer, can be used for simultaneously prevention and the treatment of cerebral thrombosis.Compare with Western medicine, good effect of the present invention, toxic and side effects is little, expense is low, and clinic is applied.
Modern study:
Alzheimer disease pathologic is characterized as senile plaque (aβ protein is assembled formation), neurofibrillary tangles (Tau abnormal protein phosphatization).Oxidation can induce aβ protein to assemble, and there is the oxidation product of protein the neurofibrillary tangle forming part, illustrates that oxidation has participated in the whole course of disease of AD.Therefore can be from improving brain blood flow state, the formation of antagonism oxidation reaction two aspects inhibition senile plaque, the effect of prevention and treatment AD is played by neuroprotective unit.
Radix Puerariae mainly contains flavone compound, can improve the oxygen metabolism of cardiac muscle, and the energy blood vessel dilating improves microcirculation simultaneously, reduces vascular resistance, and blood flow is increased, and hypertension, hyperlipidemia, hyperglycemia and cardiovascular and cerebrovascular disease are had significant curative effect.Wherein Radix Puerariae isoflavone can effectively stop the genesis of cerebral tissue and lipids in serum peroxidization, and improves SOD activity intensity in the body, animal groups is woven with protects preferably effect; Puerarin can resist the neurotoxicity of amyloid beta protein, makes the learning and memory raise marks of model mice.
Radix Notoginseng mainly contains saponins compound, and the energy expansion of cerebral vascular strengthens cerebrovascular flow, has antioxidation, antiinflammatory, the anti-ageing effect of waiting for a long time.Notoginseng saponins Rg 1 can reduce the content of AChE activity, MDA and lipofuscin in the cerebral cortex, with study, the memory function of improving brain.
Rhizoma Chuanxiong mainly contains alkaloid compound, and the energy expansion of cerebral vascular reduces vascular resistance, significantly increases brain and LBF amount, improves microcirculation, reduces surface activity of blood platelet, suppresses platelet aggregation, the formation of pre-preventing thrombosis.Ligustrazine can make D-ga1 and NaNO
2Combined induction, AlCl
3Induce two kinds of AD model mouse escape latency to shorten, AChE activity decreased, the active raising of SOD, MDA content can obviously reduce A in two kinds of AD model mouse cerebral tissue in the cerebral tissue
β, NF-
κThe expression of B.
Flos Carthami mainly contains flavone compound, the energy anticoagulant, and the fortifying fibre protein dissolution reduces whole blood viscosity; Injection, ethanol extract, saffloside can significantly improve hypoxia-bearing capability, and the weary oxygen encephalopathy (HIE) of ischemia is had protective effect.Carthamus yellow wherein has obvious effect of scavenging radical.
Semen Persicae can obviously increase cerebral blood flow, can make hemorrhage and clotting time obviously prolongs, and external thrombus is had inhibitory action, and fiber promotion, antioxidation, antiinflammatory, antibiotic, anti-allergic effects are arranged.The protein PR-B that is separated in its water-soluble component has great SOD sample active.
The gypenoside blood pressure lowering, blood fat reducing, blood sugar lowering, arteriosclerosis, inhibition thrombosis can also strengthen the activity of blood of human body medium-sized lymphocyte, strengthen the immunologic function of human body.Can improve AD rat model ability of learning and memory.
Fructus Crataegi flavone has more lasting antioxidation; More sure effect for reducing fat is arranged.Can reduce the area of cerebral ischemic reperfusion in rats cerebral infarction, reduce the expression of MMP-9, the performance cerebral protection.
The present invention has the increase cerebral blood flow, improves microcirculation, strengthens the tolerance to cerebral ischemia, improves antioxidation system; Reduce whole blood viscosity and plasma viscosity, the effects such as anticoagulant and thrombosis.
The specific embodiment
Be used for the preparation method of the medicine of prevention and treatment Alzheimer and cerebral thrombosis, get crude drug by weight proportion: Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part is made medical material fragmentation only;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, and makes medicine by the industry routine.
Be used for the preparation method of the medicine of prevention and treatment Alzheimer and cerebral thrombosis, get crude drug by weight proportion: Radix Notoginseng 30-120 part, Rhizoma Chuanxiong 30-120 part, Flos Carthami 30-120 part, Semen Persicae 30-120 part, Radix Puerariae 90-360 part, Fructus Crataegi flavone 50-150 part, gypenoside 50-150 part; Medical material is made only fragmentation;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, with Fructus Crataegi flavone, gypenoside mix homogeneously according to the above ratio, make medicine by the industry routine.
The present invention will be further described below in conjunction with embodiment, and following each embodiment only is used for explanation the present invention and is not limitation of the present invention, and the preparation method that does not wherein have to narrate all is according to the conventional preparation of industry.
Embodiment 1: the preparation of tablet
Get crude drug by weight proportion: Radix Notoginseng 120g, Rhizoma Chuanxiong 120g, Flos Carthami 120g, Semen Persicae 120g, Radix Puerariae 360g.
1, medical material is made only
Radix Notoginseng: pick and remove impurity, fragmentation;
Rhizoma Chuanxiong: pick and remove impurity, section;
Flos Carthami: gauze is decocted a drug wrapped;
Semen Persicae: pick and remove impurity, smash to pieces;
Radix Puerariae: pick and remove impurity, section;
2, extraction process is determined
Select water for extracting solvent, carry out the test of four factors, three horizontal quadratures.Four factors are followed successively by extraction time (h), extraction time, amount of water, blank.Three levels of every factor.Take the thick paste amount (weight coefficient 0.3) of product and index thing content (weight coefficient 0.7) as criterion, carry out statistical analysis, draw best extraction production technology.The Orthogonal Experiment and Design scheme sees Table 1, and result of the test sees Table 2, and the results of analysis of variance sees Table 3.
Table 1 extraction process orthogonal design factor level table
Table 2 extraction process L
9(3)
4Orthogonal experiments
Table 3 extraction process orthogonal test the results of analysis of variance
Result of the test shows that the influence factor is followed successively by B＞A＞C from big to small, and wherein the B factor has highly significant (＜0.01) to test, and the A factor has significance (＜0.05), and the C factor is less on the test impact.Orthogonal table shows that optimum extraction process is A
2B
2C
2, but data show C
1And C
2Be more or less the same, consider from the angle of saving cost, final Optimization Technology is that 8 times of water gagings decoct 2 times, each 2 hours, i.e. and A
2B
2C
1Optimum condition is verified the orthogonal experiment selection process accordingly, the results are shown in Table 4:
Table 4 extraction process orthogonal test selection process the result
| Numbering | Inventory (g) | Paste volume (g) | Content (%) |
| 1 | 720 | 743.50 | 0.44 |
| 2 | 720 | 728.10 | 0.48 |
| 3 | 720 | 733.35 | 0.46 |
3. dry
The medicinal liquid for subsequent use that extracts is at temperature 70-90 ℃, and under the vacuum 0.03-0.07Mpa, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, 70 ℃ of dryings, and dried cream is pulverized, and crosses 100 mesh sieves, and is for subsequent use.
4. mix
Get dried cream powder and the Radix Notoginseng fine powder mixes according to the equivalent incremental method, and get final product.
5. the selection of wetting agent
Because medicine viscosity ratio of the present invention is larger, need to adopt suitable wetting agent soft material processed, the present invention has selected ethanol and has adopted the ethanol soft material processed of variable concentrations to carry out the screening of suitable wetting agent, and 14 purpose stainless steel meshs are granulated, and 60 ℃ of dryings the results are shown in Table 5:
Table 5 different concentration ethanol result of the test
| Concentration of alcohol | Character | Soft material viscosity | The granule homogeneity | Pellet hardness | The tabletting quality |
| 80％ | Color and luster is uneven | Greatly | Generally | Greatly | Poor |
| 85％ | Color and luster is uneven | Greatly | Generally | Greatly | Generally |
| 90％ | Color and luster is consistent | Moderate | Uniform particles | Moderate | Well |
| 95％ | Color and luster is uneven | Little | Generally | Little | Generally |
The result shows that uniform particles, hardness that 90% ethanol makes are moderate, and the quality of tabletting is better, so select it.
In sum, this preparation process is defined as: medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and adds suitable adjuvant, stirs, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 2: the preparation of tablet
Get crude drug by weight proportion: Radix Notoginseng 30g, Rhizoma Chuanxiong 30g, Flos Carthami 30g, Semen Persicae 30g, Radix Puerariae 90g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and adds suitable adjuvant, stirs, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 3: the preparation of tablet
Get crude drug by weight ratio: Radix Notoginseng 49g, Rhizoma Chuanxiong 49g, Flos Carthami 57.5g, Semen Persicae 57.5g, Radix Puerariae 164g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and adds suitable adjuvant, stirs, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 4: the preparation of tablet
Get crude drug by weight proportion: Radix Notoginseng 120g, Rhizoma Chuanxiong 120g, Flos Carthami 120g, Semen Persicae 120g, Radix Puerariae 360g, Fructus Crataegi flavone 150g, gypenoside 150g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and Fructus Crataegi flavone, gypenoside mix homogeneously with according to the above ratio add suitable adjuvant, stir, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 5: the preparation of tablet
Get crude drug by weight proportion: Radix Notoginseng 49g, Rhizoma Chuanxiong 49g, Flos Carthami 57.5g, Semen Persicae 57.5g, Radix Puerariae 164g, Fructus Crataegi flavone 90g, gypenoside 90g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and Fructus Crataegi flavone, gypenoside mix homogeneously with according to the above ratio add suitable adjuvant, stir, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 6: the preparation of tablet
Radix Notoginseng 60g, Rhizoma Chuanxiong 60g, Flos Carthami 65g, Semen Persicae 65g, Radix Puerariae 180g, Fructus Crataegi flavone 75g, gypenoside 75g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and Fructus Crataegi flavone, gypenoside mix homogeneously with according to the above ratio add suitable adjuvant, stir, granulate, granulate, tabletting, coating is made 1000 in tablet.
Embodiment 7: the preparation of capsule
Get crude drug by weight proportion: Radix Notoginseng 30g, Rhizoma Chuanxiong 30g, Flos Carthami 30g, Semen Persicae 30g, Radix Puerariae 90g, Fructus Crataegi flavone 50g, gypenoside 50g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and Fructus Crataegi flavone, gypenoside mix homogeneously with according to the above ratio add suitable adjuvant, stir, and granulate, and granulate is filled, and makes 1000 of capsules.
Embodiment 8: the preparation of granule
Get crude drug by weight proportion: Radix Notoginseng 60g, Rhizoma Chuanxiong 60g, Flos Carthami 70g, Semen Persicae 70g, Radix Puerariae 200g, Fructus Crataegi flavone 60g, gypenoside 60g.
Medical material is made only, fragmentation.Get according to the above ratio Radix Notoginseng powder and be broken into fine powder, for subsequent use; Get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8 times of water gagings and decoct extraction 2 times, each 2 hours, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, and is for subsequent use;
Other gets Radix Notoginseng fine powder for subsequent use and thick paste mixing, 70 ℃ of dryings, and dried cream powder is broken to sieve, and Fructus Crataegi flavone, gypenoside mix homogeneously with according to the above ratio add suitable adjuvant, stir granulation agent 1000g.
The present invention can also make other suitable pharmaceutical dosage form.
Pharmacological research:
1, embodiment 1-6 is on the impact of rat platelet cohesion function
80 of male rats about body weight 220g, are divided into 8 groups, 10 every group at random: 1. model group is to normal saline, 2. aspirin group, and 3. embodiment 1, and 4. embodiment 2, and 5. embodiment 3, and 6. embodiment 4, and 7. embodiment 5, and 8. embodiment 6.Dosage such as following table, ig administration, 1 time/d, continuous 7d (aspirin group 4d); 1h after the last administration, anesthesia, the ventral aorta blood sampling, preparation platelet rich plasma and platelet poor plasma are measured Platelet aggregation in the 5min with platelet aggregation instrument.The results are shown in Table 6:
Table 6 preparation of the present invention on rat platelet cohesion function affect the result (
N=10)
Annotate: compare with model group,
※P＜0.01,
ZeroP＜0.05.
The result shows that preparation of the present invention has obvious inhibitory action to the maximum coagulation rate of rat platelet.
2, most preferred embodiment 5 is on the impact of rat platelet cohesion function
50 of male rats about body weight 220g, are divided into 5 groups, 10 every group at random: 1. model group, give the 2. 3. 4. 5. heavy dose of group of embodiment 5 preparations of dosage group in embodiment 5 preparations of embodiment 5 preparation small dose group of aspirin group of normal saline.Dosage such as following table, ig administration, 1 time/d, continuous 7d (aspirin group 4d); 1h after the last administration, anesthesia, the ventral aorta blood sampling, preparation platelet rich plasma and platelet poor plasma are measured Platelet aggregation in the 5min with platelet aggregation instrument.The results are shown in Table 7:
Table 7 preparation of the present invention on rat platelet cohesion function affect the result (
N=10)
Annotate: compare with matched group,
※P＜0.01,
ZeroP＜0.05.
The result shows that preparation of the present invention has obvious inhibitory action to the maximum coagulation rate of rat platelet.
3, on the impact of blood stasis model rat blood rheological characteristic
60 of rats, male and female half and half, about body weight 220g, divide equally 6 groups, 10 every group: 1. normal group, give the 2. 3. 4. real 6. heavy dose of group of embodiment 5 preparations of dosage group of 5. executing in example 5 preparations of embodiment 5 preparation small dose group of aspirin group of model group of normal saline.Dosage such as following table, ig administration, 1 time/d, continuous 8d (aspirin administration 5d), 30min after the 7d administration, sc epinephrine 0.9mg/kg, the rearmounted ice-water bath cooling of 30min 8min dries water with it, sc equivalent epinephrine again behind first time Injection of Adrenaline 4h, water is can't help in fasting after processing, 60min after last administration in the 8th day, under anesthesia, ventral aorta blood sampling 5ml, put and survey its whole blood viscosity and Plasma Viscosity in the blood viscosity instrument, the results are shown in Table 8:
Table 8 preparation of the present invention on blood stasis model rat blood rheological characteristic affect the result (
N=10)
Annotate: compare with model group,
※P＜0.01,
ZeroP＜0.05.
The result shows that single equal contrast group of all indexs of adrenergic rat of giving obviously raises, and shows the model establishment.Administration group rat index all has reduction in various degree, shows the preparation of the present invention blood stasis effect that has clear improvement.
4, on the impact of aged mouse anti-oxidation function
Aged kunming mice was 40 in 15 months, about body weight 40g, was divided at random 4 groups, 10 every group: 1. model group, give the 2. 3. 4. heavy dose of group of embodiment 5 preparations of dosage group in embodiment 5 preparations of embodiment 5 preparation small dose group of normal saline.Dosage such as following table, ig administration, 1 time/d, continuous 1 month.After the off-test, get the mouse brain tissue, preparation brain tissue homogenate liquid, thiobarbituricacidα-spectrophotometry lipid peroxide product malonaldehyde (MDA) content, the xanthine oxidase of improvement is measured superoxide dismutase (SOD) content.The results are shown in Table 9:
Table 9 preparation of the present invention on the aged mouse anti-oxidation function affect the result (
N=10)
Annotate: compare with matched group,
※P＜0.05.
Experimental result shows, MDA content in the mouse brain tissue, and SOD content improves, and shows that Chinese medicine preparation of the present invention is improved the effect of anti-oxidation function.
Claims (2)
1. be used for the preparation method of the medicine of prevention and treatment Alzheimer and cerebral thrombosis, it is characterized in that: get crude drug by weight proportion: 30-120 parts of Radix Notoginseng, 30-120 parts of Rhizoma Chuanxiongs, 30-120 parts on Flos Carthami, 30-120 parts in Semen Persicae, 90-360 parts of Radix Puerariaes, medical material is made only fragmentation;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, and makes medicine by the industry routine;
Or: get crude drug by weight proportion: 30-120 parts of Radix Notoginseng, 30-120 parts of Rhizoma Chuanxiongs, 30-120 parts on Flos Carthami, 30-120 parts in Semen Persicae, 90-360 parts of Radix Puerariaes, 50-150 parts of Fructus Crataegi flavone, 50-150 parts of gypenosides; Medical material is made only fragmentation;
1) get according to the above ratio Radix Notoginseng, be ground into fine powder, for subsequent use;
2) get according to the above ratio the Flos Carthami of Rhizoma Chuanxiong, Semen Persicae, Radix Puerariae, gauze parcel, add 8-12 times of water gaging and decoct extraction 1-3 time, each 1-3 hour, merge water extraction liquid, filter, relative density is the thick paste of 1.30-1.35 when being evaporated to temperature 60 C, thick paste and 1) a fine powder mix homogeneously for subsequent use, 60-80 ℃ of drying, dried cream powder is broken to sieve, with Fructus Crataegi flavone, gypenoside mix homogeneously according to the above ratio, make medicine by the industry routine.
2. the preparation method of the medicine for prevention and treatment Alzheimer and cerebral thrombosis according to claim 1, it is characterized in that: wherein said medicine is tablet, capsule or granule.
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