CN102349518B - Sterilization composition containing cyproconazole and flumorph - Google Patents
Sterilization composition containing cyproconazole and flumorph Download PDFInfo
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Abstract
The invention relates to a sterilization composition containing cyproconazole and flumorph. Active ingredients of the sterilization composition are the cyproconazole and the flumorph which are compounded; the weight percentage of the two active ingredients is (90: 1)-(1: 90), preferably (60: 5)-(1: 45); the total weight percentage of the active ingredients of the sterilization composition is 1 to 90 percent, preferably 8 to 70 percent; and other ingredients are auxiliary elements available and acceptable in pesticides. The sterilization composition can be made into wettable powder, suspending agent, soluble liquid, missible oil and emulsion in water and water dispersible granules. The sterilization composition is mainly used for preventing and treating fungous diseases of crops, such as vegetables, fruit trees, grain crops and the like.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of cyproconazole and composite bactericidal composition of flumorph of containing.
Background technology
Cyproconazole, chemical name: 2-(4-chlorphenyl)-3-cyclopropyl-1-(1,2, the 4-triazol-1-yl) fourth-2-alcohol, belong to the triazole bactericide, be systemic fungicide, effect with prevention and treatment, be ergosterol demethylation inhibitor, all effective to the mould Pseudomonas of Erysiphe, Rust, spore, Septoria, Venturia germ on cereal crop, coffee, beet fruit tree and grape, can prevent and treat cereal and coffee rust, cereal, fruit tree and grape powdery mildew, peanut, beet tops pinta, apple scab and peanut white rot.Single dose is used for a long time, and germ easily produces resistance.Chemical structural formula is:
Flumorph belongs to morpholine class disinfectant use in agriculture, it is the Novel efficient agricultural bactericide of Shenyang chemical institute initiative invention, the checking such as give birth to through indoor biometrics test, field that test is tested, demonstration test, extend trial, malicious reality are tested, result shows that its activity is high, efficacy stability, to crop, the mankind, Environmental security, Social benefit and economic benefit is good, has broad application prospects.Effective especially to Peronospora, epidemic disease mycin germ.To the Oomycete on grape, potato and tomato, especially Peronosporaceae and Phytophthora bacterium have very strong fungicidal effectiveness.
Flumorph is mainly used in preventing and treating the microbial disease of Oomycete cause of disease such as cucumber downy mildew, potato late blight, tomato late blight, Chinese cabbage downy mildew, downy mildew of garpe, capsicum epidemic disease, tobacco epidemic disease, lichee epidemic disease and soybean phytophthora root rot etc.Its effect characteristics are: active high, consumption is low, to crop safety, treatment and prolection have both, the lasting period is long; the bacterial strain or the sensitive strain that the acid amide fungicides such as metalaxyl are produced resistance all have fabulous activity, and drug effect is better than existing same veriety on market.
Cyproconazole and flumorph all have higher bactericidal activity, but through after long-term independent use, the plant germ is developed immunity to drugs, cause the problems such as dosage increasing, preventive effect reduction, lasting period shortening, the Reusability of agricultural chemicals causes the degradation rate of agricultural chemicals in soil to descend, and is unfavorable for very much environment sustainable development.Yet carrying out reasonably compositely with the active ingredient of the different mechanisms of action, is to delay the disease very effective common method that develops immunity to drugs, and can be according to the effect in production application, judge this composite be synergistic effect or antagonism.Two kinds of formulas that the agriculture chemical compounding compatibility is good can significantly improve preventive effect, greatly reduce the dosage of agricultural chemicals, can also enlarge fungicidal spectrum, improve germicidal efficiency.I research staff of company passes through the long term test research in the agriculture chemical compounding field, chance on cyproconazole and flumorph composite, good synergistic effect is arranged within the specific limits, germicidal efficiency improves greatly, can be good at delaying the generation of disease resistance, can well solve pesticide resistance and the high problem of use cost of agricultural chemicals in the market, significant to agricultural production, completed immediately the present invention.And composite about cyproconazole and flumorph through preliminary search, there is not yet relevant report at home and abroad at present.
Summary of the invention
based on above technical background, in order to make the agricultural chemicals upgrading, more efficient, delay the generation of field drug-fastness, reduce peasant's drug cost, to environment environmental protection more, friendly, to satisfy the requirement of agricultural production, I research staff of company passes through the screening study of lot of experiments formula, a kind of new and effective agricultural bactericidal composition has been proposed finally, two yuan of composite bactericidal compositions that namely carry out as active ingredient take cyproconazole and flumorph, be used for preventing and treating agriculture vegetables, fruit tree, the fungal disease of seeding corn and other crops, especially outstanding to the downy mildew control efficiency, and can delay the drug-fast generation of germ, can enlarge fungicidal spectrum, reduce drug cost, improve actual preventive effect.
Specifically, the present invention is a kind of bactericidal composition preparation that is mixed as two yuan of active ingredients take cyproconazole and flumorph, wherein in bactericidal composition preparation of the present invention, the mass percent of two kinds of active ingredient cyproconazoles and flumorph is 90: 1～1: 90, on some concrete proportioning tests bases, the mass percent of active ingredient cyproconazole and flumorph can be preferably 60: 5～1: 45.Just there is no synergistic effect but can not be equal to other compound proportions, do not do formulation screening test, just synergistic effect is more obvious in this preferable range, and emphasis has added some detailed inquiry experiments.
As another important technology characteristics of the present invention, the formulations of pesticide that pesticide sterilizing composite preparation of the present invention can be prepared into are wetting powder, suspending agent, soluble liquid, missible oil, aqueous emulsion and water dispersible granules, the percentage that wherein in preparation, the gross mass of two kinds of active ingredients accounts for the whole quality of the pharmaceutical preparations is 1%～90%, can be more preferably 8%～70%, all the other prepare complementary component for agricultural chemicals used additives and formulation.
Wherein necessary component and the simple preparation implementation method about the formulations of pesticide is as follows:
When bactericidal composition of the present invention is wetting powder, the mass percent of component is: cyproconazole 1～90%, flumorph 1～90%, dispersant 3～20%, wetting agent 3～10%, filler 10～70%, press above-mentioned formula with cyproconazole and flumorph and dispersant, wetting agent and filler mixing, uniform stirring in stirred tank is mixing after airslide disintegrating mill, namely can be made into the wetting powder of the present composition.
When bactericidal composition of the present invention is suspending agent, the mass percent of component is: cyproconazole 1～90%, flumorph 1～90%, dispersant 5～20%, antifreezing agent 1～5%, thickener 0.1～2%, defoamer 0.1～0.8%, water surplus is supplied, and first other auxiliary agents is mixed, and mixes through high speed shear, add cyproconazole and flumorph, abrading-ball is 2～3 hours in ball crusher, makes a diameter all below 5mm, makes the suspending agent preparation that contains the bactericidal composition of cyproconazole and flumorph of the present invention.
When bactericidal composition of the present invention was aqueous emulsion, the mass percent of component was: cyproconazole 1～90%, flumorph 1～90%, emulsifier 3～30%, solvent 5～15%, stabilizing agent 2～15%, antifreezing agent 1～5%, defoamer 0.1～8%, thickener 0.2～2%, water is supplied.The pure and mild flumorph of former cyclome azoles, solvent and emulsifier, cosolvent are added together, make to be dissolved into uniform oil phase; With part water, antifreeze, other the insecticides adjuvant such as antimicrobial mixes into uniform water; When the reactor high speed stirs, oil phase is added water, slowly add water until reach the phase inversion point, open clipper and carry out high speed shear, and add remaining water, shear about half an hour, form the aqueous emulsion of oil-in-water type.Namely make the aqueous emulsion of the bactericidal composition that contains cyproconazole and flumorph.
When bactericidal composition of the present invention was water dispersible granules, the mass percent of component was: cyproconazole 1～90%, flumorph 1～90%, dispersant 3～20%, wetting agent 3～10%, disintegrant 2～5%, filler 10～70%.Cyproconazole, flumorph and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add and carry out granulation, drying, screening in the fluidized bed prilling dryer by sample analysis, make the water dispersible granules that contains the bactericidal composition of cyproconazole and flumorph of the present invention.
When described bactericidal composition of the present invention was missible oil, the mass percent of component was: cyproconazole 1～90%, flumorph 1～90%, emulsifier 1～15%, solvent complementary component.The missible oil preparation is dissolved in solvent according to cyproconazole and the flumorph of formula rate with capacity, adds surfactant (using after heating for dissolving in case of necessity) and other components, mixes and namely makes the said composition cream preparation.
Emulsifier described in the preparation of above-mentioned formulation is selected from any one or more mixtures with the arbitrary proportion composition in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether.
Described solvent is any one or more mixed solvents that form with arbitrary proportion in dimethylbenzene or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, pull open one or more in powder flumorph X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, glycerine.
Described defoamer is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10One or more in the aliphatic alcohols compound.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate.
Pesticide sterilizing composite of the present invention is all effective to the mould Pseudomonas of Erysiphe, Rust, spore, Septoria, Venturia germ on cereal crop, coffee, beet fruit tree and grape, can prevent and treat cereal and coffee rust, cereal, fruit tree and grape powdery mildew, peanut, beet tops pinta, apple scab and peanut white rot.Also can be used for preventing and treating tens kinds of diseases such as downy mildew, epidemic disease, anthracnose, canker, fusarium wilt, leaf spot, angular leaf spot of infringement fruit tree, vegetables, cotton and grain and oil crop.
Bactericidal composition of the present invention is compared similar medicament many advantages, first, can greatly improve the preventive effect to controlling fungal diseases of crop, especially downy mildew, epidemic disease, powdery mildew there are special efficacy, the second, drug effect is more lasting, and be difficult for by rain drop erosion, quick-acting is compared single dose also very big raising, and the 3rd, greatly delayed drug-fast generation, reduced agricultural chemicals Reusability number of times, reduce the agricultural chemicals usage amount, reduce peasant's use cost, the 4th, low-residual, lasting period easily degraded in soil later, consumption is few, and is more friendly to environment.
Embodiment:
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to absolutely not these examples.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) is all mass ratio.Should be pointed out that all any modifications of making within the spirit and principles in the present invention, be equal to replacement and improvement etc., within all should being included in protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.In all formulas, percentage all is weight percentage.The processing technology of the various preparations of the present composition is prior art, can change to some extent according to different situations.
Formulation prepares example
Embodiment 1: the processing of cyproconazole and hexaconazole suspending agent and embodiment
Cyproconazole 28g, flumorph 20g, dispersant 14.6g, antifreezing agent 4.8g, thickener 2.4g, defoamer 0.84g, water surplus complements to 300g, other auxiliary agents are mixed, mix through high speed shear, add cyproconazole and flumorph, abrading-ball is 2～3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation that contains the bactericidal composition of cyproconazole and flumorph of the present invention.
Cyproconazole 10g, flumorph 8g, dispersant 5g, antifreezing agent 2.4g, thickener 1.1g, defoamer 0.63g, water surplus complements to 100g, other auxiliary agents are mixed, mix through high speed shear, add cyproconazole and flumorph, abrading-ball is 2～3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation that contains the bactericidal composition of cyproconazole and flumorph of the present invention.
Embodiment 2: the processing of cyproconazole and hexaconazole aqueous emulsion and embodiment
Cyproconazole 7g, flumorph 10g, emulsifier 1.8g, solvent 4.5g, stabilizing agent 2.1g, antifreezing agent 1g, defoamer 0.2g, thickener 0.4g, water complements to 100g.The pure and mild flumorph of former cyclome azoles, solvent and emulsifier, cosolvent are added together, make to be dissolved into uniform oil phase; With part water, antifreeze, other the insecticides adjuvant such as antimicrobial mixes into uniform water; When the reactor high speed stirs, oil phase is added water, slowly add water until reach the phase inversion point, open clipper and carry out high speed shear, and add remaining water, shear about half an hour, form the aqueous emulsion of oil-in-water type.Namely make the aqueous emulsion of the bactericidal composition that contains cyproconazole and flumorph.
Embodiment 3: the processing of cyproconazole and diniconazole water dispersible granule and embodiment
Cyproconazole 9g, flumorph 11g, dispersant 2.7g, wetting agent 3.51g, disintegrant 2.66g, filler 65g.Cyproconazole, flumorph and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add and carry out granulation, drying, screening in the fluidized bed prilling dryer by sample analysis, make the water dispersible granules that contains the bactericidal composition of cyproconazole and flumorph of the present invention.
Embodiment 4: processing and the embodiment of cyproconazole and own azoles alcohol missible oil
Cyproconazole 6g, flumorph 13g, two kinds of emulsifier 6g, two kinds of solvents are supplied 100g.Be dissolved in solvent according to cyproconazole and the flumorph of formula rate with capacity, add surfactant (using after heating for dissolving in case of necessity) and other components, mix and namely make the said composition cream preparation.
Indoor virulence and field experiment
The synergy test that cyproconazole and flumorph are composite.It is tested object that Plasmopara viticola is adopted in test.
Concrete grammar is:
(1) test medicine
The 95% former medicine of Cyproconazole, Fengdeng Pesticide Co., Ltd., Jiangsu, the 95% former medicine of flumorph, Shenyang Kechuang chemicals Co., Ltd.
Cyproconazole and flumorph dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of lower low temperature, and be stand-by, is diluted to a series of concentration with sterile water before test.
(2) subjects
The downy mildew of garpe germ, separation and purification gained from the grape incidence of leaf of Daze Mount vineyard, Qingdao is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCL0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L.
(3) test method
Plasmopara viticola after preculture on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates under 25 ℃ of dark conditions.Every processing repeats 3 times.When the control strain colony diameter grows to 6.5cm when above, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single doses suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single dose cyproconazole and flumorph are carried out toxicity test, both EC in order to upper method
50Value is respectively 6.42mg/L and 5.12mg/L.
Test was respectively 4: 46,12: 38,20: 30,28: 22,36: 14,44: 6 with cyproconazole flumorph mass ratio, totally 6 combinations that are mixed.
(4) data statistics and analysis
Carry out the toxicity test of mixture according to mixed ratio with single dose toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun processed
50Value is converted into actual mixed toxicity index (ATI); According to the proportioning of mixture, obtain theoretical mixed toxicity index (TTI), calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single dose toxicity index=(standard medicament EC
50/ reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in mixed of the content (%) of toxicity index * A of TII=A in mixed+B
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is large 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
(5) toxicity test result and analysis
The composite virulence to Plasmopara viticola of table 1 cyproconazole and flumorph
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyproconazole | Y＝1.3658X+3.8971 | 0.9912 | 6.42 |
| Flumorph | Y＝1.5687X+3.8873 | 0.9876 | 5.12 |
| Cyproconazole: flumorph is 4: 46 | Y＝1.7536X+40231 | 0.9911 | 3.61 |
| Cyproconazole: flumorph is 12: 38 | Y＝1.4566X+42270 | 0.9634 | 3.39 |
| Cyproconazole: flumorph is 20: 30 | Y＝1.5328X+4.1338 | 0.9875 | 3.67 |
| Cyproconazole: flumorph is 28: 22 | Y＝1.6027X+3.9998 | 0.9779 | 4.21 |
| Cyproconazole: flumorph is 36: 14 | Y＝1.5971X+3.9490 | 0.9741 | 4.55 |
| Cyproconazole: flumorph is 44: 6 | Y＝1.4355X+4.0055 | 0.9836 | 4.93 |
The composite co-toxicity to Plasmopara viticola of table 2 cyproconazole and flumorph
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyproconazole | 1∶0 | 100.00 | 100.00 | / | / |
| Flumorph | 0∶1 | 125.39 | 123.36 | / | / |
| Cyproconazole+flumorph | 4∶46 | 178.03 | 119.30 | 144.32 | Synergy |
| Cyproconazole+flumorph | 12∶38 | 189.17 | 115.23 | 158.57 | Synergy |
| Cyproconazole+flumorph | 20∶30 | 174.76 | 111.17 | 151.66 | Synergy |
| Cyproconazole+flumorph | 28∶22 | 152.57 | 107.11 | 137.24 | Synergy |
| Cyproconazole+flumorph | 36∶14 | 141.10 | 103.05 | 131.73 | Synergy |
| Cyproconazole+flumorph | 44∶6 | 130.24 | 123.36 | 126.39 | Synergy |
According to bactericide joint toxicity measuring result, cyproconazole and flumorph mass ratio are the co-toxicity coefficient all obviously large 120 of 4: 46,12: 38,20: 30,28: 22,36: 14,44: 6 o'clock, all show as synergistic effect, especially co-toxicity coefficient is up to 158.57 when cyproconazole and flumorph mass ratio are 12: 38, and we think that its synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 12: 38 left and right scopes of suitable proportion, to estimate its field practical application effect.
Experimental example 2: experimental subjects is still the downy mildew of garpe germ.
Method is roughly with above experiment.
On the basis of preliminary experiment, respectively single dose cyproconazole and flumorph are carried out toxicity test, both EC in order to upper method
50Value is respectively 5.13mg/L and 4.69mg/L.
Test was respectively 4: 16,6: 14,8: 12,10: 10,12: 8,14: 6 with 20% cyproconazole flumorph mass ratio, totally 6 combinations that are mixed.
Toxicity test result and analysis
The composite virulence to Plasmopara viticola of table 3 cyproconazole and flumorph
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyproconazole | Y＝2.1634X+3.4637 | 0.9854 | 5.13 |
| Flumorph | Y＝2.3544X+3.4197 | 0.9713 | 4.69 |
| Cyproconazole: flumorph is 4: 16 | Y＝2.4967X+3.7356 | 0.9639 | 3.21 |
| Cyproconazole: flumorph is 6: 14 | Y＝2.5007X+3.8531 | 0.9597 | 2.87 |
| Cyproconazole: flumorph is 8: 12 | Y＝2.4993X+3.8172 | 0.9846 | 2.97 |
| Cyproconazole: flumorph is 10: 10 | Y＝2.3758X+3.8009 | 0.9918 | 3.20 |
| Cyproconazole: flumorph is 12: 8 | Y＝2.2854X+3.7842 | 0.9877 | 3.40 |
| Cyproconazole: flumorph is 14: 6 | Y＝2.3006X+3.7159 | 0.9779 | 3.62 |
The composite co-toxicity to Plasmopara viticola of table 4 cyproconazole and flumorph
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyproconazole | 1∶0 | 100.00 | 100.00 | / | / |
| Flumorph | 0∶1 | 109.38 | 109.38 | / | / |
| Cyproconazole+flumorph | 4∶16 | 159.85 | 107.51 | 148.69 | Synergy |
| Cyproconazole+flumorph | 6∶14 | 178.45 | 106.57 | 167.45 | Synergy |
| Cyproconazole+flumorph | 8∶12 | 172.53 | 105.63 | 163.34 | Synergy |
| Cyproconazole+flumorph | 10∶10 | 160.48 | 104.69 | 153.29 | Synergy |
| Cyproconazole+flumorph | 12∶8 | 150.72 | 103.75 | 145.27 | Synergy |
| Cyproconazole+flumorph | 14∶6 | 141.89 | 102.81 | 138.01 | Synergy |
According to bactericide joint toxicity measuring result, 20% cyproconazole and flumorph mass ratio are the co-toxicity coefficient all obviously large 120 of 4: 16,6: 14,8: 12,10: 10,8: 12,14: 6 o'clock, all show as synergistic effect, especially co-toxicity coefficient is up to 167.45 when cyproconazole and flumorph mass ratio are 6: 14, and we think that its synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 6: 14 left and right scopes of suitable proportion, to estimate its field practical application effect.
Test example 1: field trial is measured the control efficiency to downy mildew of garpe
(1) test method
Test dispenser twice altogether, check plot spray equivalent clear water.When making up a prescription, after first with a small amount of water, medicament fully being dissolved, then add suitable quantity of water to carry out complete stool spraying processing, even spraying.All adopting power driven sprayer, operating pressure during the spray medicine is 0.3～0.4MPa, shower nozzle aperture 1.3mm.The residential quarter spouting liquid is 40～50kg/667m
2, dispenser twice continuously, sprays application to the crop complete stool uniformly with liquid at 10 days intervals.
(2) investigation method
Experimental field be located at the Qingdao Pingdu City, select occurring degree medium vineyard for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every residential quarter, look into 5 caves at every, and during investigation, fixing 2, the middle part of every strain blade fixes a point to check.
Come classification according to the downy mildew of garpe grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.
The downy mildew of garpe grade scale is
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before medicine, preventive effect investigation in 10 days after the 1st medicine, 10 days and preventive effect investigation in 20 days after 2 medicines.
(3) poisoning investigation
After dispenser, whether continuous 7d range estimation medicament has poisoning to crop.
(4) drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index after disease index before blank district medicine * treatment region medicine)/(after blank district medicine before disease index * treatment region medicine disease index)) * 100
(5) field control effectiveness test result of the test
Each test medicine of table 5 is prevented and treated the experiment effect of downy mildew of garpe
As shown in Table 5, it is more remarkable that 20% cyproconazole and flumorph are that the combination that is mixed of 6: 14 prevents and treats the effect of downy mildew of garpe, spray for the first time that 10 days best preventive effects are 88.82% after medicine, spray for the second time that 10 days best preventive effects are 93.98% after medicine, best preventive effect after 20 days is 90.18%, and the effect of complex preparation significantly is better than two kinds of single doses, has not only significantly improved the control efficiency for downy mildew, and further extended its lasting period, and under observing, the poisoning of continuous 7 days produces without poisoning.
To sum up test described, of the present invention take cyproconazole and flumorph as the composite bactericidal composition preparation of active ingredient, aspect the fungal disease of control vegetables, fruit tree, seeding corn and other crops, control efficiency is preferably being arranged all, especially the control efficiency to downy mildew is outstanding, simultaneously to crop safety, to the disease control of the field crops such as vast vegetables, fruit tree, corn, reduce the peasant drug cost, increasing both production and income is significant, and suggestion is promoted the use of.
Claims (4)
1. one kind contains cyproconazole and the composite bactericidal composition of flumorph, it is characterized in that: this bactericidal composition preparation be carry out take cyproconazole and flumorph as active ingredient two yuan composite, wherein the mass ratio of active ingredient cyproconazole and flumorph is 4:16; 6:14; 8:12; 10:10; 12:8; 14:6; 4:46; 12:38; 20:30; 28:22; 36:14; 44:6.
2. bactericidal composition according to claim 1, it is characterized in that: the gross mass of active ingredient cyproconazole and flumorph accounts for 1%～90% of whole preparation gross mass.
3. bactericidal composition according to claim 2, it is characterized in that: the gross mass of active ingredient cyproconazole and flumorph accounts for 8%～70% of whole preparation gross mass.
4. according to claim 1 and 2 or 3 described bactericidal compositions, it is characterized in that: the pesticidal preparations formulation that this bactericidal composition can be mixed with is wetting powder, suspending agent, soluble liquid, missible oil, aqueous emulsion and water dispersible granules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110339718 CN102349518B (en) | 2011-11-01 | 2011-11-01 | Sterilization composition containing cyproconazole and flumorph |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110339718 CN102349518B (en) | 2011-11-01 | 2011-11-01 | Sterilization composition containing cyproconazole and flumorph |
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| Publication Number | Publication Date |
|---|---|
| CN102349518A CN102349518A (en) | 2012-02-15 |
| CN102349518B true CN102349518B (en) | 2013-05-15 |
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| CN 201110339718 Active CN102349518B (en) | 2011-11-01 | 2011-11-01 | Sterilization composition containing cyproconazole and flumorph |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4849439A (en) * | 1983-03-04 | 1989-07-18 | Sandoz Ltd. | α-phenyl-α-cyclopropylalkyene-1H-imidazole- and 1,2,4 triazole-ethanols as antimycotic agents |
| EP0520585A1 (en) * | 1991-06-28 | 1992-12-30 | Shell Internationale Researchmaatschappij B.V. | Fungicidal compositions |
| CN1043720C (en) * | 1995-08-28 | 1999-06-23 | 化学工业部沈阳化工研究院 | Fungicide contg. fluorodiphenyl acrylamides |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4849439A (en) * | 1983-03-04 | 1989-07-18 | Sandoz Ltd. | α-phenyl-α-cyclopropylalkyene-1H-imidazole- and 1,2,4 triazole-ethanols as antimycotic agents |
| EP0520585A1 (en) * | 1991-06-28 | 1992-12-30 | Shell Internationale Researchmaatschappij B.V. | Fungicidal compositions |
| CN1043720C (en) * | 1995-08-28 | 1999-06-23 | 化学工业部沈阳化工研究院 | Fungicide contg. fluorodiphenyl acrylamides |
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