CN102346180A - Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding - Google Patents
Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding Download PDFInfo
- Publication number
- CN102346180A CN102346180A CN2011101510898A CN201110151089A CN102346180A CN 102346180 A CN102346180 A CN 102346180A CN 2011101510898 A CN2011101510898 A CN 2011101510898A CN 201110151089 A CN201110151089 A CN 201110151089A CN 102346180 A CN102346180 A CN 102346180A
- Authority
- CN
- China
- Prior art keywords
- transgenic
- genetically modified
- index
- modified plants
- feeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a method for evaluating the immunological safety of transgenic brown rice as a young broiler ration material used for feeding. The method provided by the invention comprises the following steps: (1) comparing components and component contents of nutrients between a transgenic plant and a non-transgenic control parent; and proceeding step (2) to (4) for further detection if the differences are not substantial; (2) feeding laboratory animals respectively with feedstuffs containing the transgenic plant and containing the non-transgenic control parent; (3) carrying out index detections upon an executed laboratory animal 1 and an executed laboratory animal 2; (3) comparing the indexes in the step (3) between the laboratory animal 1 and the laboratory animal 2; and determining that the transgenic plant is immunologically safe to living things as a candidate if the differences of all the indexes are not substantial. According to the method for detecting the immunological safety of transgenic plants to animals fed with the transgenic plants, the detection indexes are more comprehensive, the detection results are corresponded to reality, and the results are more reliable. Therefore, the method provided by the invention has wide application prospect in the field of immunological safety detection of transgenic plants to animals fed with the transgenic plants.
Description
Technical field
The present invention relates to a kind of method of estimating transgenosis brown rice as the feeding immune security of meat chick day grain raw material.
Background technology
Because genetically modified crops are demonstrating great potential aspect the improvement proterties such as resistance, quality, output; China has started the great special project of genetically modified organism rearing new variety in 2008; Be devoted to cultivate, promote a collection of genetically modified organism new varieties that application potential is arranged, yet the prerequisite of wideling popularize is that it is carried out safety evaluatio.Genetically modified crops are used as food and animal feed, thereby feeding safety evaluatio is an importance of genetically modified crops safety evaluatio.
Summary of the invention
An object of the present invention is to provide the method for a kind of auxiliary detection genetically modified plants to the immune security of biology.
Auxiliary detection genetically modified plants provided by the present invention comprise the steps: the method for the immune security of biology
1) genetically modified plants and non-transgenic component and the component concentration significant difference whether that contrasts the nutritional labeling between the parent relatively; If significant difference, then candidate ground confirms that said genetically modified plants are to biological right and wrong immunity safety; If difference is not remarkable, then carry out following steps 2) to 4) with further detection;
2) respectively with the feed that comprises said genetically modified plants or the said non-transgenic contrast parent animal used as test of feeding; To do experiment animal 1 with the animal used as test note that the feed that comprises said genetically modified plants is fed, will do experiment animal 2 with the animal used as test note that the feed that comprises said non-transgenic contrast parent is fed;
3) animal used as test 1 after putting to death and animal used as test 2 being carried out following index respectively detects:
The organ index: organ index=organ weight/live body is heavy; Said organ is liver, thymus gland and/or the bursa of farbricius;
Whole blood index: red blood cell number, leucocyte number, neutrophil leucocyte number, neutrophil leucocyte number percent, monocyte number, monocyte number percent, lymphocyte number, lymphocyte number percent, T lymphocyte transformation rate and bone-marrow-derived lymphocyte conversion ratio;
Serological index: serum lysozyme activity, newcastle epidemic disease antibody titre, gpt activity, glutamic-oxalacetic transaminease activity, total protein concentration, globulin concentration, A/G, immunoglobulin A concentration, immunoglobulin G concentration, immunoglobulin M concentration;
Intestinal villi medium size lymphocyte quantity; Said small intestine is duodenum, jejunum or ileum;
IL-2 content, IL-6 content or IFN-γ content in the jejunum tissue;
4) comparison step 3) in each index significant difference whether between animal used as test 1 and animal used as test 2;
If the equal difference of all indexs is not remarkable, then candidate ground confirms that said genetically modified plants are immunity safety to biology;
If at least one index significant difference then carries out following steps 5 to the index of significant difference)-7) further detect:
5) with not containing normal diet that genetically modified plants and non-transgenic contrast the parent animal used as test of feeding; To do experiment animal 3 with the animal used as test note that the normal diet that does not contain genetically modified plants and non-transgenic contrast parent is fed;
6), detect the index of significant difference described in the step 4) respectively to the animal used as test 3 after putting to death;
7) comparison step 4) described in the index of significant difference significant difference whether between animal used as test 1 and animal used as test 3; If difference is all not remarkable, then candidate ground confirms that said genetically modified plants are immunity safety to biology; If have an index significant difference at least, then candidate ground confirms that said genetically modified plants are to biological right and wrong immunity safety;
Said immunity safety is meant said genetically modified plants immune safety to biology on feeding.
The animal of said execution can existing any atraumatic execution method (being the non-surgery operation method) obtain.
In the said method, said biology is for being the animal of food with said genetically modified plants.
In the said method, the plant that sets out the when parent of said genetically modified plants is the preparation genetically modified plants.
In above-mentioned arbitrary said method, the component of said nutritional labeling is water, albumen, fat, carbohydrates, NFE, fiber, ash content, calcium, phosphorus, amino acid.
In above-mentioned arbitrary said method, said genetically modified plants are transgenic crop; Said animal used as test is the meat chick.
In above-mentioned arbitrary said method, said transgenic crop is a transgenic paddy rice, and being specially changes the CrylAc/sck trans-genetic hybrid rice.
In above-mentioned arbitrary said method, said meat chick is the chicken of 1 age in days, body weight 42 grams.
In above-mentioned arbitrary said method, meter when beginning from feeding carried out said index respectively and detects, and compares each index of the 21st day and each index of the 42nd day respectively at the 21st day and the 42nd day; Remembered the said same day of feeding and did the 1st day.
In above-mentioned arbitrary said method, the 1st day to the 21st day used feed is following in the said process of feeding:
The composition and the mass parts of the said normal diet that does not contain genetically modified plants and non-transgenic contrast parent are following: the big dregs of beans of corn 57.6, non-transgenic 31.27, corn oil 2.52, fish meal 4.79, calcium monohydrogen phosphate 1.07, salt 0.3, stone flour (lime carbonate) 1.37, DL-methionine 0.08, premix 1;
The composition and the mass parts of the said feed that comprises said genetically modified plants are following: the big dregs of beans of said genetically modified plants 57.2, non-transgenic 32.84, corn oil 1.63, fish meal 4.53, (calcium monohydrogen phosphate) 1.04, salt 0.27, lime carbonate 1.42, DL-methionine 0.07, premix 1;
The composition and the mass parts of the said feed that comprises said non-transgenic contrast parent are following: the big dregs of beans of said non-transgenic contrast parent 58.9, non-transgenic 31.35, corn oil 1.4, fish meal 4.56, calcium monohydrogen phosphate 1.02, salt 0.27, lime carbonate 1.43, DL-methionine 0.07, premix 1;
In above-mentioned arbitrary said method, the 22nd day to the 42nd day used feed is following in the said process of feeding:
The composition and the mass parts of the said normal diet that does not contain genetically modified plants and non-transgenic contrast parent are following: the big dregs of beans of corn 58.8, non-transgenic 30.25, corn oil 4.32, fish meal 3.03, (calcium monohydrogen phosphate) 1.1, salt 0.22, lime carbonate 1.27, DL-methionine 0.01, premix 1;
The composition and the mass parts of the said feed that comprises said genetically modified plants are following: the big dregs of beans of said genetically modified plants 58.6, non-transgenic 31.77, corn oil 3.26, fish meal 2.78, calcium monohydrogen phosphate 1.06, salt 0.19, lime carbonate 1.34, premix 1;
The composition and the mass parts of the said feed that comprises said non-transgenic contrast parent are following: the big dregs of beans of said non-transgenic contrast parent 59.86, non-transgenic 30.92, corn oil 3.15, fish meal 2.43, calcium monohydrogen phosphate 1.11, salt 0.2, lime carbonate 1.33, premix 1;
The 1st day to the 21st day used premix composition and proportioning is following in the said process of feeding: vitamin A 12500IU, vitamin D
34400IU, vitamin E 15mg, vitamin K
33.8mg, Cobastab
11.5mg, Cobastab
27.4mg, Cobastab
62.4mg, Cobastab
120.01mg, calcium pantothenate 8.8mg, niacinamide 25mg, folic acid 0.75mg, biotin 0.10mg, iron 70mg, copper 11mg, manganese 70mg, zinc 65mg, iodine 0.49mg, selenium 0.3mg;
The 22nd day to the 42nd day used premix composition and proportioning is following in the said process of feeding: vitamin A 10000IU, vitamin D
33500IU, vitamin E 12mg, vitamin K
33.0mg, Cobastab
11.2mg, Cobastab
26.0mg, Cobastab
62mg, Cobastab
120.008mg, calcium pantothenate 7.0mg, niacinamide 20mg, folic acid 0.60mg, biotin 0.08mg, iron 63mg, copper 10mg, manganese 65mg, zinc 59mg, iodine 0.45mg, selenium 0.28mg.
Above-mentioned arbitrary said method is also belonging to protection scope of the present invention to the application in the feeding safety evaluatio of genetically modified plants.
The present invention relates to changeing a kind of new method of CrylAc/sck gene brown rice as the feeding immune safety evaluatio of meat chick day grain raw material; Through the use of this method, for transgenosis brown rice provides a cover technological assessment new method as the feeding immunity safety of meat chick day grain raw material.
Genetically modified plants of the present invention to food the detection method of immune security of animal, it is more comprehensive to detect index, makes testing result more meet reality, more credible.Therefore, the inventive method genetically modified plants to the immune security detection range of the animal of food and have broad application prospects in the feeding safety evaluatio field of genetically modified plants.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Experiment material is following:
Change CrylAc/sck trans-genetic hybrid rice (name is called MSA) and in document " changeing crylAc/sck gene pest-resistant paddy rice estimates the field influence of the main non-target pest in rice field ", disclosed, the public can obtain from processing of farm products research institute of the Chinese Academy of Agricultural Sciences;
The parent's (name is called MH86) who changes the CrylAc/sck trans-genetic hybrid rice disclosed in document " transgenic pest-resistant rice is to the influence of rice grub group ", and the public can obtain from processing of farm products research institute of the Chinese Academy of Agricultural Sciences;
The meat chick is the chicken of 1 age in days, body weight 42 grams, all plants Ji Chang available from Beijing China.
The immune security of CrylAc/sck trans-genetic hybrid rice to the meat chick changeed in embodiment 1, evaluation
The nutritional labeling contrast of step 1, commentaries on classics CrylAc/sck gene brown rice and parent's brown rice
Change CrylAc/sck gene brown rice and be grown in the adjacent field with parent's brown rice, growth conditions is consistent, plant, be exposed to the sun, separately strict each other in storage and the process, avoid any possible mixing and pollution, preserve before the test in the shady and cool ventilation drying and locate.Two kinds of paddy rice are processed into brown rice, are used for Determination of nutritional components and preparation meat chick full-value nutritious diet after the pulverizing.Feed sample collecting method (GB/T 14699. 1-93) is gathered transgenosis paddy and its non-transgenic parent sample (being grain) respectively, pulverizes the back and crosses 40 mesh sieves.Carry out following composition measurement then, each sample is surveyed three times and is repeated, and gets its mean value at last.
1, moisture, dry, crude protein, crude fat, robust fibre, coarse ash and NFE content in the working sample, assay method is with reference to " forage analysis and feeding quality detection technique " (Zhang Liying work).
Moisture: sample is placed in 105 ℃ of baking ovens dries to constant weight, the weightlessness of sample is promptly represented the content of moisture.
Crude protein content: adopt the semi-automatic azotometer of Kai Shi to carry out titration.
Crude fat content: with the ether is that extraction agent carries out the Suo Shi extraction.
Coarse ash content: the residue obtained coarse ash that is after 550 ℃ of calcinations.
Crude fiber content: adopt acid-base method to measure.
NFE content=1-moisture-crude protein content-crude fat content-coarse ash content-crude fiber content.
Determination of Amino Acids Content: adopt acid hydrolysis (6mol/L hydrochloric acid; 110 ℃ of hydrolysis 24h) and oxydrolysis (methionine, cystine use peroxyformic acid to carry out oxidation processes) two kinds of brown rice are handled, use Beckman 6300 type automatic amino acid analyzers to measure 17 seed amino acid content.Each sample is surveyed three times and is repeated, and gets its mean value at last.
The result: in nutritional labeling and the content comparison, each index does not all have significant difference, shows that commentaries on classics CrylAc/sck trans-genetic hybrid rice and parent paddy rice have substantial equivalence property.Concrete outcome such as table 1 and table 2.
Transgenosis brown rice and parent's thereof conventional nutritional labeling is seen table 1.From table, can find out in the transgenosis brown rice content of crude protein, crude fat, coarse ash, carbohydrates, NFE and robust fibre, calcium and phosphorus in the conventional nutritional labeling; Slightly variant with its parent; All be lower than the content of each composition in parent's brown rice, but difference not significantly (P＞0.05).
Table 1, transgenic paddy rice and parent's thereof conventional nutritional labeling
Annotate: be significant difference with the P of delegation＜0.05 in the table, P＜0.01 is that difference is extremely remarkable.
17 seed amino acids and total amino acid content content are seen table 2 in transgenosis brown rice and the parent's brown rice thereof.From table, can find out valine in the transgenosis brown rice (Val) and alanine (Ala) content a little more than parent's brown rice, but difference not remarkable (P＞0.05); Other 15 seed amino acid content all are lower than parent's brown rice, and other 14 seed amino acid content are a little less than parent's brown rice, but difference not significantly (P＞0.05).
Content of amino acids in table 2, transgenosis brown rice and the parent's brown rice thereof
Annotate: the content of thick protein * 100% in various content of amino acids/brown rice in the amino acid composition=brown rice.
Step 2, the meat chick of feeding
1, the equipping rules method of feed
The preparation of test daily ration is with reference to fryer nutritional need among the agricultural industry criteria NY/T 33-2004 of the People's Republic of China (PRC) " fowl raising standard ", and is according to various nutrient composition content preparation brown rice-dregs of beans type daily rations in the brown rice of measuring, specific as follows:
Table 3, meat chick basal diet prescription
Premix in early stage composition: vitamin A 12500IU; Vitamin D
34400IU; Vitamin E 15mg; Vitamin K
33.8mg; Cobastab
11.5mg; Cobastab
27.4mg; Cobastab
62.4mg; Cobastab
120.01mg; Calcium pantothenate 8.8mg; Niacinamide 25mg; Folic acid 0.75mg; Biotin 0.10mg; Iron 70mg; Copper 11mg; Manganese 70mg; Zinc 65mg; Iodine 0.49mg; Selenium 0.3mg.
Later stage premix composition: vitamin A 10000IU; Vitamin D
33500IU; Vitamin E 12mg; Vitamin K
33.0mg; Cobastab
11.2mg; Cobastab
26.0mg; Cobastab
62mg; Cobastab
120.008mg; Calcium pantothenate 7.0mg; Niacinamide 20mg; Folic acid 0.60mg; Biotin 0.08mg; Iron 63mg; Copper 10mg; Manganese 65mg; Zinc 59mg; Iodine 0.45mg; Selenium 0.28mg.
Under the prerequisite that does not influence broiler growth, add with the alternative corn formula feed of rice with maximum albumen during formula feed, other raw materials are the non-transgenic product in the daily ration, and daily ration is fed with the powder form.
2, the raising of meat chick and experimental design
360 1d AA meat chicks are divided three groups of raisings, and 120 every group, 6 repetitions, 20 of every repetitions (public female half and half); The experimental group transgenosis brown rice daily ration (transgenosis group) of feeding; Control group fed non-transgenic brown rice daily ration (parent's group), the corn group corn daily ration (corn group) of feeding.Adopt 3 layers of solid to raise in cages, each processed group is uniformly distributed in the meat chick house of artificially controlling temperature, and feeding and management and immune programme for children are by " AA commercial meat bird feeding and management handbook is carried out, and freely drinks water free choice feeding.Whole test phase 42d divides two stages, and 1-21d and 22-42 remembered and do the 1st day the same day of feeding.
Step 3, index detect
1, organ sampling method and time
From each repetition of each processed group, got one of chicken at random respectively in the 21st day and the 42nd day, and weighed on an empty stomach, the neck bloodletting is slaughtered.Get liver, thymus gland, the bursa of farbricius, organ with the blood above the filter paper exhaustion, is removed the chicken feather and other foreign material (adipose connective tissue etc.) be stained with above the organ, be put into weigh on the electronic balance reading and record then.Calculate the organ index as follows:
Organ index=organ weight (g)/live body heavy (kg).
2, whole blood sampling method and time
Select the fasting of meat chicken respectively at selecting for the 20th age in days evening 7 with the 41st age in days evening 7,12h on an empty stomach gets one of chicken at random from each of each processed group repeats; Marked; 7 of the 21st age in days mornings and the 42nd age in days point in mornings 7 are put to death chicken, take a blood sample immediately then.Detect following whole blood immunity evaluation index:
Red blood cell number, leucocyte number, neutrophil leucocyte number, neutrophil leucocyte number percent, monocyte number, monocyte number percent, lymphocyte number, lymphocyte number percent, T lymphocyte transformation rate and bone-marrow-derived lymphocyte conversion ratio.
3, serum sampling method and time
Select the fasting of meat chicken respectively at selecting for the 20th age in days evening 7 with the 41st age in days evening 7,12h on an empty stomach gets one of chicken at random from each of each processed group repeats; Marked; 7 of the 21st age in days mornings and the 42nd age in days point in mornings 7 are put to death chicken, take a blood sample immediately then.Preparation serum, it is subsequent use to put into-20 ℃ of refrigerators.Detect serological index: serum lysozyme activity, newcastle epidemic disease antibody titre, gpt activity, glutamic-oxalacetic transaminease activity, total protein concentration, globulin concentration, A/G (ball/white), immunoglobulin A concentration, immunoglobulin G concentration, immunoglobulin M concentration.
4, small intestine sampling method and time
Respectively 21, repeat 1 chicken of picked at random from each during 42d and butcher; Get duodenum (Pai Yierjie place), jejunum (1cm place before the yolk stalk) and each 1cm of ileum (3cm place before the ileocecal opening) fast; Place 4% formalin fixing immediately, routine paraffin wax embedding, section.Get jejunum and organize 2g in the Axgen centrifuge tube ,-80 ℃ of preservations are subsequent use.Detect following intestine immunity evaluation index: small intestine (duodenum, jejunum, ileum) fine hair medium size lymphocyte quantity, IL-2, IL-6, IFN-γ content in the jejunum tissue.
Intestinal villi medium size lymphocyte quantity; Said small intestine is duodenum, jejunum or ileum.
Each index in step 4, the comparison step three
1, the significant difference whether between transgenosis group and parent group of each index in the comparison step three;
If the equal difference of all indexs is not remarkable, then candidate ground confirms that commentaries on classics CrylAc/sck gene brown rice is safe as meat chick day grain raw material in feeding immunity;
If at least one index significant difference then carries out following steps 2 relatively to the index of significant difference:
2, the index of the significant difference in the comparison step 1 significant difference whether between transgenosis group and control group;
If index difference between transgenosis group and corn group of the significant difference in the step 1 is not remarkable, then also candidate ground confirms that commentaries on classics CrylAc/sck gene brown rice is safe as meat chick day grain raw material in feeding immunity;
If at least one index difference between transgenosis group and corn group is also remarkable in the index of the significant difference in the step 1, then candidate ground confirms that commentaries on classics CrylAc/sck gene brown rice is unsafe as meat chick day grain raw material in feeding immunity;
Result for each index in the step 3 all estimates with statistical method.Statistics is with average ± standard error (X ± SE) expression.Adopt the multiple check program among the SAS of statistical software 8.0 editions that data are carried out significance test of difference.
The result:
Transgenosis brown rice is to the influence of meat chick 21d immune organ index
| Index | The transgenosis group | Parent's group | The corn group |
| Thymus index | 3.64±0.32a | 3.11±0.23a | 3.28±0.17a |
| Index and spleen index | 0.78±0.05a | 0.70±0.06a | 0.73±0.06a |
| Bursa of farbricius index | 2.23±0.14a | 2.16±0.15a | 2.30±0.17a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 42d immune organ index
| Index | The transgenosis group | Parent's group | The corn group |
| Thymus index | 3.08±0.30a | 2.98±0.28a | 3.78±0.44a |
| Index and spleen index | 0.83±0.06a | 0.93±0.09a | 1.21±0.15a |
| Bursa of farbricius index | 1.16±0.13a | 0.97±0.12a | 1.11±0.11a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 21d whole blood index
| The whole blood index | The transgenosis group | Parent's group | The corn group |
| RBC red blood cell count(RBC) (10 12/L) | 2.48±0.03a | 2.52±0.04a | 2.49±0.09a |
| WBC white blood cell count(WBC) (10 9/L) | 129.70±4.72a | 130.30±4.60a | 128.55±5.18a |
| GR neutrophil leucocyte (10 9/L) | 2.35±0.08a | 2.37±0.08a | 2.25±0.09a |
| GR% granulocyte percent (%) | 44.05±1.57b | 38.73±2.88b | 28.02±3.02a |
| MID intercalated nucleus cell (10 9/L) | 0.28±0.06a | 0.35±0.02a | 0.27±0.07a |
| MID% intercalated nucleus cell percentage (%) | 2.58±0.87b | 2.6±0.86b | 1.68±0.14a |
| LY lymphocyte (10 9/L) | 116.63±3.44a | 115.22±3.37a | 114.65±2.06a |
| LYMF lymphocyte number percent (%) | 16.87±0.73b | 16.70±0.59b | 12.76±0.57a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 42d whole blood index
| The whole blood index | The transgenosis group | Parent's group | The corn group |
| RBC red blood cell count(RBC) (10 12/L) | 2.48±0.04a | 2.44±0.04a | 2.45±0.06a |
| WBC white blood cell count(WBC) (10 9/L) | 113.44±5.94a | 108.12±5.61a | 114.19±2.95a |
| GR neutrophil leucocyte (10 9/L) | 2.25±0.09a | 2.37±0.08a | 2.35±0.08a |
| GR% granulocyte percent (%) | 33.87±0.68b | 38.73±2.88ab | 44.05±1.57a |
| MID intercalated nucleus cell (10 9/L) | 2.25±0.09a | 2.37±0.08a | 2.35±0.08a |
| MID% intercalated nucleus cell percentage (%) | 2.05±0.20a | 1.80±0.12a | 2.22±0.15a |
| LY lymphocyte (10 9/L) | 106.17±4.42a | 104.87±4.86a | 107.83±6.46a |
| LYMF lymphocyte number percent (%) | 12.70±0.57b | 14.87±0.86ab | 16.87±0.73a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 21d serum biochemistry index
| The serum index | The transgenosis group | Parent's group | The corn group |
| ALT glutamic-pyruvic transaminase (U/L) | 2.50±0.22a | 2.67±0.21a | 2.33±0.21a |
| AST glutamic-oxalacetic transaminease (U/L) | 221.50±5.12a | 216.33±11.39a | 211.33±5.93a |
| TP total protein (g/L) | 30.32±0.85ab | 32.98±1.16b | 27.65±1.27a |
| Alb globulin (g/L) | 14.58±0.28a | 15.52±0.28a | 14.15±0.72a |
| A/G (ball/white) | 0.93±0.04ab | 0.88±0.06b | 1.03±0.02a |
| IgA immunoglobulin A (mg/dL) | 0.95±0.23a | 1.15±0.39a | 1.38±0.24a |
| IgG immunoglobulin G (mg/dL) | 6.64±0.51a | 5.69±0.28a | 6.26±0.31a |
| IgM immunoglobulin M (mg/dL) | 13.21±0.99ab | 15.55±0.28b | 11.83±1.52a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 42d serum biochemistry index
| The serum index | The transgenosis group | Parent's group | The corn group |
| ALT glutamic-pyruvic transaminase (U/L) | 2.50±0.22a | 2.50±0.22a | 2.67±0.21a |
| AST glutamic-oxalacetic transaminease (U/L) | 275.00±27.86a | 265.30±12.93a | 237.17±11.99a |
| TP total protein (g/L) | 37.17±3.03a | 36.27±4.79a | 33.37±0.87a |
| Alb globulin (g/L) | 16.82±0.75a | 16.20±0.49a | 16.57±0.42a |
| A/G ball/white | 0.90±0.08a | 0.98±0.07a | 0.92±0.05a |
| IgA immunoglobulin A (mg/dL) | 0.65±0.09a | 0.76±0.19a | 0.84±0.12a |
| IgG immunoglobulin G (mg/dL) | 1.98±0.10a | 1.65±0.28a | 1.87±0.18a |
| IgM immunoglobulin M (mg/dL) | 18.01±0.48a | 17.68±0.37a | 17.09±0.42a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to meat chick 21d and 42d serum lysozyme active (U/mL) influence
| Age in days | The transgenosis group | Parent's group | The corn group |
| 21d | 215.21±15.43a | 228.87±17.39a | 219.54±16.76a |
| 42d | 303.56±20.72a | 310.89±21.65a | 315.82±22.91a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 21d and 42d serum newcastle epidemic disease antibody titre
| Age in days | The transgenosis group | Parent's group | The corn group |
| 21d | 2.33±0.15a | 2.43±0.12a | 2.33±0.21a |
| 42d | 3.43±0.06a | 3.47±0.11a | 3.47±0.13a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence of meat chick 42d periphery blood T lymphocyte and bone-marrow-derived lymphocyte conversion ratio
| Index | The transgenosis group | Parent's group | The corn group |
| The T lymphocyte transformation rate | 0.83±0.06a | 0.82±0.04a | 0.90±0.05a |
| The bone-marrow-derived lymphocyte conversion ratio | 0.85±0.03a | 0.97±0.04a | 0.98±0.05a |
Transgenosis brown rice is to the influence (individual/100 columnar cell) of meat chick 21d small intestine (duodenum, jejunum, ileum) fine hair medium size lymphocyte quantity
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence (individual/100 columnar cell) of meat chick 42d small intestine (duodenum, jejunum, ileum) fine hair medium size lymphocyte quantity
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence (pg/mg) of IL-2 content in meat chick 21d and the 42d jejunum tissue
| Age in days | The transgenosis group | Parent's group | The corn group |
| 21d | 2.14±0.27a | 1.93±0.38a | 2.09±0.34a |
| 42d | 3.43±0.06a | 3.47±0.11a | 3.46±0.13a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence (pg/mg) of IL-6 content in meat chick 21d and the 42d jejunum tissue
| Age in days | The transgenosis group | Parent's group | The corn group |
| 21d | 0.13±0.02a | 0.14±0.03a | 0.13±0.03a |
| 42d | 0.34±0.05b | 0.44±0.03b | 0.37±0.03b |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Transgenosis brown rice is to the influence (pg/mg) of IFN-γ content in meat chick 21d and the 42d jejunum tissue
| Age in days | The transgenosis group | Parent's group | The corn group |
| 21d | 1.17±0.17a | 1.12±0.20a | 1.14±0.22a |
| 42d | 1.44±0.25a | 1.37±0.10a | 1.38±0.31a |
Discrepant with letter in the delegation is significant difference (P＜0.05)
Above testing result shows that changeing Cry1Ac/sck gene brown rice is safe as meat chick day grain raw material in feeding immunity.
Claims (8)
1. auxiliary detection genetically modified plants comprise the steps: the method for the immune security of biology
1) genetically modified plants and non-transgenic component and the component concentration significant difference whether that contrasts the nutritional labeling between the parent relatively; If significant difference, then candidate ground confirms that said genetically modified plants are to biological right and wrong immunity safety; If difference is not remarkable, then carry out following steps 2) to 4) with further detection;
2) respectively with the feed that comprises said genetically modified plants or the said non-transgenic contrast parent animal used as test of feeding; To do experiment animal 1 with the animal used as test note that the feed that comprises said genetically modified plants is fed, will do experiment animal 2 with the animal used as test note that the feed that comprises said non-transgenic contrast parent is fed;
3) animal used as test 1 after putting to death and animal used as test 2 being carried out following index respectively detects:
The organ index: organ index=organ weight/live body is heavy; Said organ is liver, thymus gland and/or the bursa of farbricius;
Whole blood index: red blood cell number, leucocyte number, neutrophil leucocyte number, neutrophil leucocyte number percent, monocyte number, monocyte number percent, lymphocyte number, lymphocyte number percent, T lymphocyte transformation rate and bone-marrow-derived lymphocyte conversion ratio;
Serological index: serum lysozyme activity, newcastle epidemic disease antibody titre, gpt activity, glutamic-oxalacetic transaminease activity, total protein concentration, globulin concentration, A/G, immunoglobulin A concentration, immunoglobulin G concentration, immunoglobulin M concentration;
Intestinal villi medium size lymphocyte quantity; Said small intestine is duodenum, jejunum or ileum;
IL-2 content, IL-6 content or IFN-γ content in the jejunum tissue;
4) comparison step 3) in each index significant difference whether between animal used as test 1 and animal used as test 2;
If the equal difference of all indexs is not remarkable, then candidate ground confirms that said genetically modified plants are immunity safety to biology;
If at least one index significant difference then carries out following steps 5 to the index of significant difference)-7) further detect:
5) with not containing normal diet that genetically modified plants and non-transgenic contrast the parent animal used as test of feeding; To do experiment animal 3 with the animal used as test note that the normal diet that does not contain genetically modified plants and non-transgenic contrast parent is fed;
6), detect the index of significant difference described in the step 4) respectively to the animal used as test 3 after putting to death;
7) comparison step 4) described in the index of significant difference significant difference whether between animal used as test 1 and animal used as test 3; If difference is all not remarkable, then candidate ground confirms that said genetically modified plants are immunity safety to biology; If have an index significant difference at least, then candidate ground confirms that said genetically modified plants are to biological right and wrong immunity safety;
Said immunity safety is meant said genetically modified plants immune safety to biology on feeding.
2. method according to claim 1 is characterized in that: the component of said nutritional labeling is water, albumen, fat, carbohydrates, NFE, fiber, ash content, calcium, phosphorus, amino acid.
3. according to claim 1 or 2 or 3 described methods, it is characterized in that: said genetically modified plants are transgenic crop; Said animal used as test is the meat chick.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: said transgenic crop is a transgenic paddy rice, and being specially changes the Cry1Ac/sck trans-genetic hybrid rice.
5. according to arbitrary described method among the claim 1-4, it is characterized in that: said meat chick is the chicken of 1 age in days, body weight 42 grams.
6. according to arbitrary described method among the claim 1-5, it is characterized in that: meter when beginning from feeding, carried out said index respectively and detect, and compare each index of the 21st day and each index of the 42nd day respectively at the 21st day and the 42nd day; Remembered the said same day of feeding and did the 1st day.
7. according to arbitrary described method among the claim 1-6, it is characterized in that:
The 1st day to the 21st day used feed is following in the said process of feeding:
The composition and the mass parts of the said normal diet that does not contain genetically modified plants and non-transgenic contrast parent are following: the big dregs of beans of corn 57.6, non-transgenic 31.27, corn oil 2.52, fish meal 4.79, calcium monohydrogen phosphate 1.07, salt 0.3, lime carbonate 1.37, DL-methionine 0.08, premix 1;
The composition and the mass parts of the said feed that comprises said genetically modified plants are following: the big dregs of beans of said genetically modified plants 57.2, non-transgenic 32.84, corn oil 1.63, fish meal 4.53, calcium monohydrogen phosphate 1.04, salt 0.27, lime carbonate 1.42, DL-methionine 0.07, premix 1;
The composition and the mass parts of the said feed that comprises said non-transgenic contrast parent are following: the big dregs of beans of said non-transgenic contrast parent 58.9, non-transgenic 31.35, corn oil 1.4, fish meal 4.56, calcium monohydrogen phosphate 1.02, salt 0.27, lime carbonate 1.43, DL-methionine 0.07, premix 1;
The 22nd day to the 42nd day used feed is following in the said process of feeding:
The composition and the mass parts of the said normal diet that does not contain genetically modified plants and non-transgenic contrast parent are following: the big dregs of beans of corn 58.8, non-transgenic 30.25, corn oil 4.32, fish meal 3.03, calcium monohydrogen phosphate 1.1, salt 0.22, lime carbonate 1.27, DL-methionine 0.01, premix 1;
The composition and the mass parts of the said feed that comprises said genetically modified plants are following: the big dregs of beans of said genetically modified plants 58.6, non-transgenic 31.77, corn oil 3.26, fish meal 2.78, calcium monohydrogen phosphate 1.06, salt 0.19, lime carbonate 1.34, premix 1;
The composition and the mass parts of the said feed that comprises said non-transgenic contrast parent are following: the big dregs of beans of said non-transgenic contrast parent 59.86, non-transgenic 30.92, corn oil 3.15, fish meal 2.43, calcium monohydrogen phosphate 1.11, salt 0.2, lime carbonate 1.33, premix 1;
The 1st day to the 21st day used premix composition and proportioning is following in the said process of feeding: vitamin A 12500IU, vitamin D
34400IU, vitamin E 15mg, vitamin K
33.8mg, Cobastab
11.5mg, Cobastab
27.4mg, Cobastab
62.4mg, Cobastab
120.01mg, calcium pantothenate 8.8mg, niacinamide 25mg, folic acid 0.75mg, biotin 0.10mg, iron 70mg, copper 11mg, manganese 70mg, zinc 65mg, iodine 0.49mg, selenium 0.3mg;
The 22nd day to the 42nd day used premix composition and proportioning is following in the said process of feeding: vitamin A 10000IU, vitamin D
33500IU, vitamin E 12mg, vitamin K
33.0mg, Cobastab
11.2mg, Cobastab
26.0mg, Cobastab
62mg, Cobastab
120.008mg, calcium pantothenate 7.0mg, niacinamide 20mg, folic acid 0.60mg, biotin 0.08mg, iron 63mg, copper 10mg, manganese 65mg, zinc 59mg, iodine 0.45mg, selenium 0.28mg.
Among the claim 1-7 arbitrary said method to the application in the feeding safety evaluatio of genetically modified plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101510898A CN102346180A (en) | 2011-06-07 | 2011-06-07 | Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101510898A CN102346180A (en) | 2011-06-07 | 2011-06-07 | Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102346180A true CN102346180A (en) | 2012-02-08 |
Family
ID=45545050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101510898A Pending CN102346180A (en) | 2011-06-07 | 2011-06-07 | Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102346180A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103397098A (en) * | 2013-08-13 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | Method for evaluating nutritional status of scophthalmus maximus in metamorphosis |
| CN103875608A (en) * | 2014-03-24 | 2014-06-25 | 华中农业大学 | Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis |
| CN103931564A (en) * | 2014-04-15 | 2014-07-23 | 华中农业大学 | Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae |
| CN109371060A (en) * | 2018-11-27 | 2019-02-22 | 金陵科技学院 | A kind of quick transgene method of spindle tree |
-
2011
- 2011-06-07 CN CN2011101510898A patent/CN102346180A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 杜红方: "转基因水稻作为肉仔鸡日粮原料的安全性评价", 《博士论文》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103397098A (en) * | 2013-08-13 | 2013-11-20 | 中国水产科学研究院黄海水产研究所 | Method for evaluating nutritional status of scophthalmus maximus in metamorphosis |
| CN103397098B (en) * | 2013-08-13 | 2016-03-23 | 中国水产科学研究院黄海水产研究所 | A kind of method evaluating turbot transformation reactions phase nutritional status |
| CN103875608A (en) * | 2014-03-24 | 2014-06-25 | 华中农业大学 | Safety evaluation method of transgene insect-resistant rice on predative natural enemy cyrtorhinus lividipennis |
| CN103931564A (en) * | 2014-04-15 | 2014-07-23 | 华中农业大学 | Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae |
| CN103931564B (en) * | 2014-04-15 | 2016-03-09 | 华中农业大学 | Transgenic pest-resistant rice is to parasite Anagrus nilaparvatae method for evaluating safety |
| CN109371060A (en) * | 2018-11-27 | 2019-02-22 | 金陵科技学院 | A kind of quick transgene method of spindle tree |
| CN109371060B (en) * | 2018-11-27 | 2021-09-14 | 金陵科技学院 | Method for rapid transgenosis of euonymus persicifera |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Woods et al. | A comparison of the effect of urchin size and diet on gonad yield and quality in the sea urchin Evechinus chloroticus Valenciennes | |
| Dienye et al. | Growth performance and haematological responses of African mud catfish Clarias gariepinus fed dietary levels of Moringa oleifera leaf meal | |
| Gaye-Siessegger et al. | Feeding level and individual metabolic rate affect δ 13 C and δ 15 N values in carp: implications for food web studies | |
| Saeid et al. | Effect on supplementation of Spirulina maxima enriched with Cu on production performance, metabolical and physiological parameters in fattening pigs | |
| Yadav et al. | In vivo cell-mediated immune, hemagglutination inhibition response, hematological and biochemical values in native vs. exotic chicken breeds | |
| CN102346180A (en) | Method for evaluating immunological safety of transgenic brown rice as young broiler ration material used for feeding | |
| Tan et al. | Effects of supplemental A zomite in diets on growth, immune function and disease resistance of white shrimp (L itopenaeus vannamei) | |
| Ko et al. | The produced mealworm meal through organic wastes as a sustainable protein source for weanling pigs | |
| Akapo et al. | Effect of feeding cassava (Manihot esculenta Crantz) root meal on growth performance, hydrocyanide intake and haematological parameters of broiler chicks | |
| Saragih et al. | Effects of Spirogyra jaoensis as a dietary supplement on growth, pectoralis muscle performance, and small intestine morphology of broiler chickens | |
| Ojediran et al. | Blood parameters, carcass yield, organ weight and villi morphometrics of broilers fed low protein diet in excess of dietary lysine | |
| Chakraborty et al. | Earthworm meal (Perionyx excavatus) as an alternative protein source to fish meal in feed for juvenile butter catfish (Ompok pabda) | |
| CN101861955A (en) | Preparation method of plant feed additive for improving immunity of layer chicken | |
| Afia et al. | Haematological profile and growth response of African sharptooth catfish (Clarias gariepinus, Burchell 1822) fingerlings to locally formulated and commercial pelleted diets in tarpaulin tanks | |
| Manyelo et al. | The Influence of different amaranth leaf meal inclusion levels on performance, blood profiles, and gut organ characteristics of Ross 308 broiler chickens | |
| Sobhi et al. | Influence of combined yeast-derived zinc, selenium and chromium on performance, carcass traits, immune response and histomorphological changes in broiler chickens | |
| Agina et al. | Haemato-biochemical profile of apparently healthy domestic turkeys (Meleagris gallopavo) in Nsukka, Enugu state, Nigeria | |
| Opoola et al. | Effect of Spirulina platensis supplementation on performance, haematological and serum biochemical profiles of broiler chickens reared under tropical environment | |
| Wongnhor et al. | Effects of dried chaya leaf meal inclusion in the diet on growth performance and blood profiles in Thai native chicken (Pradu Hangdum) | |
| Webb et al. | Immune function and hematology of male cotton rats (Sigmodon hispidus) in response to food supplementation and methionine | |
| Lugata et al. | Effect of genotype on the hematological parameter of TETRA-SL and Hungarian Partridge coloured chickens at young age | |
| Straková et al. | Values of selected haematological indicators in six species of feathered game | |
| Gheorghe et al. | Assessment of plasma biochemistry and intestinal microflora in Transylvanian Naked Neck breed compared with commercial breeders'. | |
| Sjollema et al. | The vitamin A, carotene and xanthophyll content of the yolk of hens' eggs | |
| Candido et al. | Comparison of clinical parameters in captive Cracidae fed traditional and extruded diets |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120208 |