CN102337315A - Method for preparing decolorized blueberry polysaccharide - Google Patents
Method for preparing decolorized blueberry polysaccharide Download PDFInfo
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- CN102337315A CN102337315A CN2011102961762A CN201110296176A CN102337315A CN 102337315 A CN102337315 A CN 102337315A CN 2011102961762 A CN2011102961762 A CN 2011102961762A CN 201110296176 A CN201110296176 A CN 201110296176A CN 102337315 A CN102337315 A CN 102337315A
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Abstract
The invention discloses a method for preparing a decolorized blueberry polysaccharide. The method comprises the steps of: S1, adding cellulase in a pretreated blueberry pulp, stirring and enzymolyzing at a temperature of 50-60 DEG C for 60-90 minutes, and then placing an enzymolysis liquid after enzymolysis into boiled water to inactivate enzyme for 1-2 minutes; S2, centrifugating the enzymolysis liquid after enzyme inactivation, taking a supernatant liquid, and decolorizing by passing the supernatant liquid through an LKS01 macroporous adsorption resin, wherein a packed column of the macroporous adsorption resin is 5-10g, the supernatant liquid is a blueberry juice desaturated by 5-10 times, the loading quantity is 400-500ml, and the flow rate is 1-3ml/min; and S3, freezing and drying for 24-48 hours after concentrating a decolorized polysaccharide solution obtained in the step S2 to the solution with the solid content value of 15-20, so as to obtain decolorized blueberry polysaccharide powder. The method for preparing the decolorized blueberry polysaccharide disclosed by the invention has the advantages of simple operation and higher polysaccharide purity up to 80-90 percent.
Description
Technical field
The present invention relates to the blueberry processing technique field, especially relate to a kind of preparation method of blueberry decolouring polysaccharide.
Background technology
Blueberry (Blueberry), the formal name used at school blueberry belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium spp) plant.Blueberry is little berry, and it is blue that fruit is, subcircular, and pulp is fine and smooth, and seed is minimum, sweet acid appropriateness, and have fragrant refreshing pleasant fragrance, and can eat raw, also can be used as the raw material of food, beverage, medicine etc.Be rich in multiple nutritional components common in the fruit in the blueberry fruit; And inhibitor and bacterial growth suppressor factor such as anthocyanin, flavonoid, belong to the fruit of homoamino acid, high zinc, high ferro, high-copper, homovitamin, have good health protection effect; Comprise vision enhancing, anti-eye strain, prevent that cranial nerve is aging, anticancer, vessel softening, enhances human body immunity etc.; Its nutritive value can be rated as " king of world's fruit " far above fruit such as apple, grape, oranges, and blueberry juice is a red-purple; Color and luster is darker, after decolouring, can solve it effectively and pollute characteristics such as skin, lip, tooth.
Polysaccharide (polysaccharide) is meant by glycosidic link bonded sugar chain, will surpass the polymerization sugar polymeric carbohydrate that the monose more than 10 is formed at least.The polysaccharide of being made up of identical monose is called holosaccharide, like starch, Mierocrystalline cellulose and glycogen; The polysaccharide of forming with different monose is called mixed polysaccharide, is made up of pentose and semi-lactosi etc. like gum arabic.Polysaccharide is not a kind of pure chemical substance, but the mixture of the different material of extent of polymerization.Polyose is generally water insoluble, and no sweet taste can not form crystallization, no reductibility and mutarotation.Polysaccharide also is a glucosides, thus can hydrolysis, in hydrolytic process, often producing a series of intermediate product, final complete hydrolysis obtains monose.
Decolouring is meant the pigment of sloughing the liquid or solid material, and the decoloring method operation of existing blueberry polysaccharide is complicated, and the purity of polysaccharide that makes is lower.
Summary of the invention
Technical problem to be solved by this invention is: operation is complicated, and the purity of polysaccharide that makes is lower.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of preparation method of blueberry decolouring polysaccharide, it comprises:
Step S1: the blueberry pulp that pre-treatment is obtained adds the Vis cellulase, under 50-60 ℃ of condition, stirs enzymolysis 60-90 minute, places boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis;
Step S2: the enzymolysis solution behind the enzyme that will go out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin; Wherein, macroporous resin dress post is 5-10g, and sample solution is dilution 5-10 blueberry juice doubly; Applied sample amount is 400-500ml, and flow velocity is 1-3ml/min;
Step S3: will be concentrated into through the polysaccharide soln of the decolouring that obtains behind the step S2 and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Further, among the said step S1, said pre-treatment step comprises: get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling.
Further, said blueberry weight ratio of gained deposition and water after pre-treatment is 1: 5-1: 10.
Further, among the said step S1, the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 1%-5%ml/100ml.
Further, said cellulase is the Vis cellulase.
Further, among the said step S1, the unit of activity of said Vis cellulase is 5.0 * 10
4U/g.
The preparation method of blueberry of the present invention decolouring polysaccharide uses enzymic degradation blueberry fiber and removes albumen promptly decolours to remove to combine with albumen and processes blueberry decolouring polysaccharide powder, and it is simple to operate, and purity of polysaccharide is higher, can reach 80%-90%.
Description of drawings
Fig. 1 is the preparing method's of blueberry decolouring polysaccharide of the present invention schematic flow sheet;
Fig. 2 is the temperature of the blueberry of the present invention decolouring polysaccharide influence contrast synoptic diagram to decolorizing effect;
Fig. 3 is the heat-up time of blueberry of the present invention decolouring polysaccharide of the influence contrast synoptic diagram to decolorizing effect;
Fig. 4 is the solid-liquid ratio of the blueberry of the present invention decolouring polysaccharide influence contrast synoptic diagram to decolorizing effect;
Fig. 5 is the sample solution concentration ratio of the blueberry of the present invention decolouring polysaccharide influence contrast synoptic diagram to decolorizing effect;
Fig. 6 is the velocity ratio of the blueberry of the present invention decolouring polysaccharide influence contrast synoptic diagram to decolorizing effect.
Embodiment
Specify the preferred embodiments of the present invention below in conjunction with accompanying drawing.
See also Fig. 1, Fig. 1 is the preparing method's of blueberry decolouring polysaccharide of the present invention schematic flow sheet.The preparation method of blueberry decolouring polysaccharide of the present invention is application enzymic degradation blueberry fiber and removes albumen and process blueberry decolouring polysaccharide powder.Said method comprises the steps:
Step S1: the blueberry pulp that pre-treatment is obtained adds cellulase, under 50-60 ℃ of condition, stirs enzymolysis 60-90 minute, places boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis;
Step S2: the enzymolysis solution behind the enzyme that will go out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin; Wherein, macroporous resin dress post is 5-10g, and sample solution is dilution 5-10 blueberry juice doubly; Applied sample amount is 400-500ml, and flow velocity is 1-3ml/min;
Step S3: will be concentrated into through the polysaccharide soln of the decolouring that obtains behind the step S2 and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Among the said step S1, said pre-treatment step comprises: get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 5-1: 10.
Among the said step S1, said cellulase is the Vis cellulase.
Among the said step S1, the unit of activity of said Vis cellulase is 5.0 * 10
4U/g, the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 1%-5%ml/100ml.
Below in conjunction with specific embodiment the present invention is further specified.
Embodiment 1
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 5.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase, under 50 ℃ of conditions, stirs enzymolysis 60-90 minute, places boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis, and the unit of activity of said Vis cellulase is 5.0 * 10
4U/g, the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 1%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 5g, and sample solution is the blueberry juice of 5 times of dilutions, and applied sample amount is 450ml, and flow velocity is 1ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Embodiment 2
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 10.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 60 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 5%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 10g, and sample solution is the blueberry juice of 5 times of dilutions, and applied sample amount is 500ml, and flow velocity is 3ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Embodiment 3
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 7.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 55 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 3%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 6g, and sample solution is the blueberry juice of 10 times of dilutions, and applied sample amount is 400ml, and flow velocity is 2ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 8.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 58 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 3%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 6g, and sample solution is the blueberry juice of 6 times of dilutions, and applied sample amount is 460ml, and flow velocity is 2ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Embodiment 5
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 6.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 58 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 4%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 8g, and sample solution is the blueberry juice of 5 times of dilutions, and applied sample amount is 500ml, and flow velocity is 3ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Embodiment 6
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 10.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 59 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 3%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 10g, and sample solution is the blueberry juice of 8 times of dilutions, and applied sample amount is 430ml, and flow velocity is 2ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Embodiment 7
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 10.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 52 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 4%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 10g, and sample solution is the blueberry juice of 5 times of dilutions, and applied sample amount is 500ml, and flow velocity is 3ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
Get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling; Said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 9.The blueberry pulp that pre-treatment is obtained adds the Vis cellulase; Under 60 ℃ of conditions, stirred enzymolysis 60-90 minute; Place boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis; The unit of activity of said Vis cellulase is 5.0 ten thousand U/g, and the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 4%ml/100ml; The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin, and wherein, macroporous resin dress post is 7g, and sample solution is the blueberry juice of 5 times of dilutions, and applied sample amount is 400ml, and flow velocity is 3ml/min; To be concentrated into through the polysaccharide soln of the decolouring that above-mentioned steps obtains and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
See also Fig. 2-Fig. 6, the present invention tests according to the size measurement that is percent of decolourization for the used macroporous adsorbent resin of decolouring.
Get no bleaching and the decolouring after fruit juice 2ml, in the 25mL volumetric flask, add deionized water; Do reference with deionized water, the percent of decolourization trial-product is carried out full wavelength scanner, confirm that maximum wavelength is 523nm; With this wavelength serves as to detect wavelength, detects the absorbancy of need testing solution, and calculates percent of decolourization.
Percent of decolourization=(absorbancy behind absorbancy-Tuo before the decolouring/take off preceding absorbancy) * 100% (I)
Experiment material is respectively AB-8, LKS05, LKS01 for blueberry juice and three kinds of macroporous adsorbent resins of five times of dilutions.
A) temperature is to the influence of decolorizing effect
Because experiment material is a fruit juice, temperature is too high can to influence the effective constituent of fruit juice, so only select 30 ℃, 40 ℃, 50 ℃ three temperature to make an experiment, adds 40ml fruit juice in the 3g wet resin, and water-bath 1 hour is brought formula (I) into and surveyed percent of decolourization.The result is as shown in Figure 2.
As can be seen from Figure 2 the decolorizing effect of LKS01 is better than LKS05, AB-8, and best temperature is 50 ℃.
B) heat-up time is to the influence of decolorizing effect
Because experiment material is a fruit juice, long-time heating can influence the effective constituent of fruit juice, thus under 50 ℃, add 40ml fruit juice in the 3g wet resin, every at a distance from absorbancy of 10min survey.The result is as shown in Figure 3.
As can beappreciated from fig. 3 the decolorizing effect of LKS01 is better than LKS05, AB-8, considers that fruit juice is difficult for long-time heating, selects 40min therefore best heat-up time.
C) solid-liquid ratio is to the influence of decolorizing effect
Add 30,40,50,60 in the 3g wet resin respectively, the fruit juice of five times of 70ml dilutions, with 130r/min,, under 523nm, survey absorbancy at 50 ℃ of following water-bath 40min, bringing formula (I) into must percent of decolourization.The result is as shown in Figure 4.
As can beappreciated from fig. 4 the decolorizing effect of resin LKS01, LKS05, AB-8 differs and is not very big, two the single factors in comprehensive front, and the decolorizing effect best resin is LKS01.
D) concentration is to the influence of percent of decolourization
The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin; Wherein, macroporous resin dress post is 5g, and sample solution is the blueberry juice of 1,3,5 times of dilution; Applied sample amount is 400ml, and collection time is the 8min/ pipe, and flow velocity is 3ml/min;
As can beappreciated from fig. 5, when sample solution concentration was the fruit juice of 5 times of dilutions, decolorizing effect was best, so the sample solution optimum concn is the fruit juice of 5 times of dilutions.
E) flow velocity is to the influence of percent of decolourization
The enzymolysis solution behind the enzyme of will going out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin; Wherein, macroporous resin dress post is 5g, and sample solution is the blueberry juice of 5 times of dilutions; Applied sample amount is 400ml, and collection time is a 8min/ pipe, and flow velocity is 1,2,3ml/min;
As can beappreciated from fig. 6, when the sample solution flow velocity was 1ml/min, percent of decolourization was maximum, so flow velocity is selected 1ml/min.
To sum up, the top condition of blueberry polychrome Crude polysaccharides preparation is: sample solution is the blueberry juice of 5 times of dilutions, and the sample solution flow velocity is 1ml/min, and macroporous adsorbent resin is the wet LKS01 of 5g, and collection time is the 8min/ pipe.Under top condition, the adsorbable 400ml fruit juice of 5g wet resin (LKS01), percent of decolourization reaches 90.28%.
The preparation method of blueberry of the present invention decolouring polysaccharide uses enzymic degradation blueberry fiber and removes albumen promptly decolours to remove to combine with albumen and processes blueberry decolouring polysaccharide powder, and it is simple to operate, and purity of polysaccharide is higher, can reach 80%-90%.
Here description of the invention and application is illustrative, is not to want with scope restriction of the present invention in the above-described embodiments.Here the distortion of the embodiment that is disclosed and change are possible, and the replacement of embodiment is known with the various parts of equivalence for those those of ordinary skill in the art.Those skilled in the art are noted that under the situation that does not break away from spirit of the present invention or essential characteristic, and the present invention can be with other form, structure, layout, ratio, and realize with other assembly, material and parts.Under the situation that does not break away from the scope of the invention and spirit, can carry out other distortion and change here to the embodiment that is disclosed.
Claims (6)
1. the preparation method of blueberry decolouring polysaccharide is characterized in that it comprises:
Step S1: the blueberry pulp that pre-treatment is obtained adds cellulase, under 50-60 ℃ of condition, stirs enzymolysis 60-90 minute, places boiling water to go out enzyme 1-2 minute enzymolysis solution behind the enzymolysis;
Step S2: the enzymolysis solution behind the enzyme that will go out carries out centrifugal, gets supernatant, and said supernatant is decoloured through the LKS01 macroporous adsorbent resin; Wherein, macroporous resin dress post is 5-10g, and sample solution is dilution 5-10 blueberry juice doubly; Applied sample amount is 400-500ml, and flow velocity is 1-3ml/min;
Step S3: will be concentrated into through the polysaccharide soln of the decolouring that obtains behind the step S2 and contain value admittedly for behind the 15-20, lyophilize 24-48h promptly obtains blueberry decolouring polysaccharide powder again.
2. the preparation method of blueberry decolouring polysaccharide according to claim 1 is characterized in that among the said step S1, said pre-treatment step comprises: get an amount of blue berry and in boiling water, scalded 1-2 minute, place under the normal temperature and smash to pieces with tissue mashing machine after the cooling.
3. the preparation method of blueberry decolouring polysaccharide according to claim 1 and 2 is characterized in that, said blueberry gained after pre-treatment precipitates and the weight ratio of water is 1: 5-1: 10.
4. the preparation method of blueberry decolouring polysaccharide according to claim 1 is characterized in that, among the said step S1, the long-pending percentage composition (addition) with the blue berry volume of slurry of enzyme liquid is 1%-5%ml/100ml.
5. the preparation method of blueberry decolouring polysaccharide according to claim 1 is characterized in that said cellulase is the Vis cellulase.
6. the preparation method of blueberry decolouring polysaccharide according to claim 5 is characterized in that the unit of activity of said Vis cellulase is 5.0 * 10
4U/g.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101704898A (en) * | 2009-09-28 | 2010-05-12 | 南京泽朗医药科技有限公司 | Process for extracting amylose of blueberry |
| CN101805766A (en) * | 2010-04-05 | 2010-08-18 | 苏州瑞蓝博中药技术开发有限公司 | Process for extracting active polysaccharide |
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2011
- 2011-10-08 CN CN2011102961762A patent/CN102337315A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101704898A (en) * | 2009-09-28 | 2010-05-12 | 南京泽朗医药科技有限公司 | Process for extracting amylose of blueberry |
| CN101805766A (en) * | 2010-04-05 | 2010-08-18 | 苏州瑞蓝博中药技术开发有限公司 | Process for extracting active polysaccharide |
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| Title |
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| 孙希云 等: "蓝莓多糖超声波提取及脱蛋白方法", 《食品科学》 * |
| 孟宪军 等: "蓝莓多糖的优化提取及抗氧化性研究", 《食品与生物技术学报》 * |
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Application publication date: 20120201 |