CN102326816A

CN102326816A - Novel SOD (Superoxide Dismutase) beverage

Info

Publication number: CN102326816A
Application number: CN2011103057370A
Authority: CN
Inventors: 刘霞; 袁勤生
Original assignee: SHANGHAI YUHUA LIFE TECHNOLOGY DEVELOPMENT Co Ltd
Current assignee: SHANGHAI YUHUA LIFE TECHNOLOGY DEVELOPMENT Co Ltd
Priority date: 2011-04-26
Filing date: 2011-10-11
Publication date: 2012-01-25

Abstract

The invention belongs to the technical field of food and particularly relates to a novel SOD (Superoxide Dismutase) beverage. The novel SOD beverage is characterized by comprising an SOD mate and a beverage component; the SOD mate and the beverage component are placed into a sealed and isolated space of a same container or are respectively placed ino different containers, wherein the SOD mate comprises SOD or SOD-like active derivatives and an activity protection agent; and the activity protection agent is a substance which has a function of protecting enzymatic activity. The novel SOD beverage provided by the invention overcomes the defects of the traditional SOD beverage that the SOD activity is not stable and the activity is easy to lose; the novel SOD beverage has the advantages of long preservation time of the SOD activity and high bioavailability so that the quality guarantee period of the SOD beverage is prolonged and the effect of the SOD beverage is improved.

Description

A kind of New type of S OD beverage

Technical field

The invention belongs to food technology field, be specifically related to a kind of New type of S OD beverage.

Background technology

Superoxide dismutase (Superoxide Dismutase; SOD) be antioxidase important in the organism; Be divided into 3 types of Cu, Zn-SOD, Fe-SOD and Mn-SOD, be distributed widely in the various organisms, its major physiological effect is to remove oxygen radical in the organism.Confirm that at present the disease that is caused by oxygen radical reaches kind more than 60, SOD can resist and block the infringement that causes because of the oxygen radical pair cell.

Each is organized and all contains SOD in the human body, but its amount and the active minimizing gradually along with the increase at age; Human body be owing to can cause a large amount of loss of SOD in each histoorgan in the body too after disease, fatigue or the strenuous exercise, thereby causes having in the body a large amount of oxygen radicals to be piled up, and causes cellular damage.This shows that the SOD of supplemented with exogenous property is very necessary to the Green Tea Extract ability that improves body, the ability that improves the body resist the disease.

SOD is as a kind of protein; Oral supplementation is fool proof effective; The liquid beverage of selling in the market that contains SOD comprises dairy produce, drinks, health care liquid, tea beverage, beverage made of fruits or vegetables, water, functional beverage etc., but factor such as contained other composition of Chang Yinqi or normal temperature resting period and active instability or the enzymatic activity of these products SOD are completely lost.

Summary of the invention

Technical problem to be solved by this invention provides a kind of New type of S OD beverage, can overcome the existing active unstable or active defective that is prone to forfeiture that contains the existing SOD of SOD beverage.

For this reason; The invention discloses a kind of New type of S OD beverage; It is characterized in that it comprises SOD companion and beverage ingredient; The two places space or the two of the airtight isolation of same container to place different vessels respectively respectively, and wherein said SOD companion comprises SOD or quasi-SOD reactive derivative and activity protecting agent, and said activity protecting agent is to have the active material of protective enzyme.

In certain embodiments, said SOD companion is made up of SOD or SOD derivative and activity protecting agent, and every gram activity protecting agent adds 2000U～10000U SOD or SOD derivative.

Wherein most preferably every gram activity protecting agent adds 10000U SOD or SOD derivative.

In certain embodiments, said activity protecting agent is a compound sugar, and said compound sugar is a disaccharides, and said disaccharides is one of in trehalose, sucrose, lactose, the maltose, wherein preferred trehalose.

In certain embodiments, said SOD is cupric and Cu/Zn SOD (Cu/Zn-SOD), contain manganese superoxide dismutase (Mn-SOD) or Fe-contained SOD (Fe-SOD), wherein preferred Cu/Zn-SOD.

In certain embodiments, said quasi-SOD reactive derivative is disclosed quasi-SOD reactive derivative of Chinese patent CN88103164.X and pharmaceutically acceptable salt thereof.

In certain embodiments, said SOD companion is freeze-dried powder or microcapsules or liposome form.

Beverage ingredient according to the invention can be conventional liq beverage or solid beverage raw material, includes but not limited to tealeaves, chrysanthemum etc.

In certain embodiments, said beverage ingredient one of includes but not limited in dairy produce, drinks, health care liquid, tea beverage, beverage made of fruits or vegetables, water, the functional beverage.

New type of S OD beverage according to the invention has overcome existing active unstable, the active defective that is prone to forfeiture that contains the existing SOD of beverage of SOD; Have the active advantage that the holding time is long, bioavailability is high of SOD, thereby prolonged the SOD beverage shelf-life and improved its effect.

The specific embodiment

Below in conjunction with specific embodiment, further set forth the present invention.These embodiment only be used to the present invention is described and be not used in the restriction scope of the present invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.Only if definition separately, employed all specialties are identical with the meaning that scientific words and one skilled in the art are familiar with in the literary composition.In addition, any with the institute similar content of putting down in writing or the equalization method and material all can be applicable in the inventive method.The usefulness that preferable implementation method described in the literary composition and material only present a demonstration.

1 one kinds of New type of S OD of embodiment companion black tea drinks

A kind of SOD companion black tea drinks comprises SOD companion dry powder and black tea drinks, and green tea beverage is placed in the beverage bottle, and the Cu/Zn-SOD freeze-dried powder is sealed in the bottle cap inner chamber by aluminium-foil paper, and SOD companion dry powder comprises red blood cell Cu/Zn-SOD 10000U and trehalose 1g.During use, destroy aluminium-foil paper, SOD companion dry powder falls into black tea, and it is several down to rock beverage bottle, promptly drinkable.

2 one kinds of New type of S OD of embodiment companion mineral water

A kind of SOD companion mineral water; Comprise SOD companion microcapsules and mineral water; Mineral water is placed in the beverage bottle; The Cu/Zn-SOD microcapsules are sealed in the bottle cap inner chamber by aluminium-foil paper, and the Cu/Zn-SOD microcapsules comprise Cu/Zn-SOD 2000U and sucrose 1g, and SOD companion microcapsules can be referring to the said method preparation of Chinese patent CN101181632.During use, destroy aluminium-foil paper, it is several down to rock beverage bottle, promptly drinkable

3 one kinds of New type of S OD of embodiment companion tealeaves

A kind of SOD companion tealeaves; Comprise SOD companion dry powder and tealeaves, tealeaves is placed in the tea can with every bag of 2g specification, and SOD companion dry powder is sealed by the vacuum aluminium foil bag; Whenever comprise 0.2 gram SOD companion dry powder, comprising red blood cell Cu/Zn-SOD 1000U and about 0.2g trehalose.During use, routine is installed brews tealeaves, tear the vacuum aluminium foil bag then, SOD companion dry powder is poured in the tea get final product, the coolant-temperature gage of advising receiving guest with tea is lower than 60 to be put into when spending, and effect is best.

Embodiment 4 enzyme activities relatively

Reagent and configuration

PH 8.250m M Tris-HCl buffer solution: 1000ml is a standard with preparation, takes by weighing Tris powder 6.057g with assay balance respectively, EDTA-2Na powder 0.372g, with the distilled water dissolved dilution to the 900ml.To pH8.2, be settled to 1000ml, stored refrigerated with the titration of HCl solution.

With 50mM 1,2,3,-thrihydroxy-benzene solution (being dissolved in 0.01N HCl): 25ml is as the criterion with preparation, takes by weighing this powder 0.1576g, with the 0.01N HCl dissolving of distilled water preparation, is settled to 25ml, the freezing preservation of shading with in advance.

Step:

The SOD vigor detects the micro-1,2,3,-thrihydroxy-benzene autoxidation method that all adopts, and concrete experimental technique is following.

At first get pH8.2050m M Tris-HCl buffer solution 3ml, wherein add Xul 50mM 1,2,3,-thrihydroxy-benzene.Pick up counting simultaneously, under the condition of λ=325nm, measure the variation of per minute absorbance in the 3min with ultraviolet-uisible spectrophotometer, be designated as Δ A company/min, reaction temperature is 25 ℃.

Secondly, carry out the SOD enzyme activity determination, get pH8.2050m M Tris-Hcl buffer solution 3ml, wherein add Xul 50mM 1,2,3,-thrihydroxy-benzene, regulate the SOD addition, make SOD suppress 1,2,3,-thrihydroxy-benzene autoxidation speed Δ A enzyme/min about 50%.

4.1 the influence that water temperature is lived to SOD companion tealeaves SOD enzyme

Get embodiment 3 said SOD companion tealeaves and conventional SOD tealeaves (per 2 gram SOD tealeaves have added red blood cell Cu/Zn-SOD 1000u), adopt hot water and cold water to make tea respectively, the SOD enzyme is lived in 10min, 40min detection tea.

Hot water is made tea (water temperature is 80 ℃), and the 10min water temperature is 60 ℃, and water temperature is 40 ℃ behind the 30min; The temperature of cold water is 25 ℃.

Result such as table one when hot water is made tea, compare with conventional SOD tealeaves, and the SOD enzyme of embodiment 3 said SOD companion tealeaves is lived and descended seldom.

Table one

4.2 the influence that room temperature standing time lives to SOD companion black tea drinks SOD enzyme

Get EXAMPLE l said SOD companion black tea drinks and conventional SOD black tea drinks (every 125mlSOD black tea drinks has added red blood cell Cu/Zn-SOD 10000U); Room temperature is placed; 6 months and 12 months; Liquid SOD enzymatic activity when detection is drunk is lived relatively with initial SOD enzyme, obtains relative SOD active (%).Result such as table two.

Table two

Scope of the present invention does not receive the restriction of said specific embodiments, and said embodiment also comprises the method and the component of functional equivalent only as the single example of illustrating various aspects of the present invention in the scope of the invention.In fact, except content as herein described, those skilled in the art can easily grasp multiple improvement of the present invention with reference to the description of preceding text.Said improvement also falls within the scope of appended claims.Every piece of list of references that preceding text are mentioned is listed this paper in as a reference all in full.

Claims

1. New type of S OD beverage; It is characterized in that it comprises SOD companion and beverage ingredient; The two places space or the two of the airtight isolation of same container to place different vessels respectively respectively; Wherein said SOD companion comprises SOD or quasi-SOD reactive derivative and activity protecting agent, and said activity protecting agent is the material with protective enzyme active function.

2. New type of S OD beverage according to claim 1 is characterized in that said SOD companion is made up of SOD or quasi-SOD derivative and activity protecting agent, and every gram activity protecting agent adds 2000U～10000USOD or quasi-SOD reactive compound.

3. like the said New type of S OD beverage of claim 2, it is characterized in that every gram activity protecting agent adds 10000U.

4. New type of S OD beverage according to claim 1 is characterized in that said activity protecting agent is a compound sugar.

5. like the said New type of S OD beverage of claim 4, it is characterized in that said disaccharides is one of in sucrose, lactose, maltose or the trehalose.

6. New type of S OD beverage according to claim 1 is characterized in that said activity protecting agent is a trehalose.

7. New type of S OD beverage according to claim 1 is characterized in that said SOD is cupric and Cu/Zn SOD, one of contains in manganese superoxide dismutase or the Fe-contained SOD or their any combinations.

8. New type of S OD beverage according to claim 1 is characterized in that said SOD companion is freeze-dried powder or microcapsules or liposome form.

9. New type of S OD beverage according to claim 1 is characterized in that said beverage is one of in dairy produce, drinks, health care liquid, tea beverage, beverage made of fruits or vegetables, water, functional beverage or the tealeaves.