CN102326677A - Composite micro-ecological feed additive and preparation method and premix thereof - Google Patents
Composite micro-ecological feed additive and preparation method and premix thereof Download PDFInfo
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Abstract
The invention discloses a composite micro-ecological feed additive and a premix thereof, and belongs to the technical field of feed science and feed additive. The feed additive contains multiple probiotics, and has the functions of enhancing the immunity of livestock and poultry and improving the production performance of the livestock and poultry; and meanwhile, the feed additive can be used as a feed mildew-proof agent, has good adhesion identifying and removing capacity on feed mycotoxins, can effectively inhibit the growth of mycete in the feed and adsorb and degrade the mycotoxins, is a novel, safe, reliable and pollution-free pure fermented multifunctional micro-ecological preparation product, and has good application prospect.
Description
Technical field
The present invention relates to a kind of composite microorganism type forage additives and premix thereof, belong to forage science and field of feed additive technology.
Background technology
Fast development along with aquaculture; People have related to various aspects to the research of feed addictive, and wherein the safety issue of antibiotic etc class feed addictive and feed have been become two big focuses of research in present feed industry and the animal husbandry production by mould and mycotoxin contamination problem.
Receive the infringement of mould according to agricultural product such as the grain in the United Nations grain tissue estimation whole world annual nearly 5%～7%, feeds, thereby caused enormous economic loss.By the feed of mould contamination, its nutriment is decomposed, and palatability descends, and produces multiple mycotoxin; Animals eat the feed of moldy metamorphism, can take place acute or the slow poisoning symptom, cause the speed of growth of livestock and poultry slow; Feed intake descends, and digestibility reduces, fertility and immunity degradation; The serious harm animal health, even cause and be poisoned to death, huge harm brought to aquaculture.
Mould inhibitor is of a great variety in the market, and common mould inhibitor has organic acid, acylate and organic acid ester and some composite mildew inhibitors.But these all are chemical anti mildew agents, and itself does not improve feed quality, in use animal are had excitant simultaneously, palatability, security and antibacterial aspect exist not enough.
The feed probiotics is as a kind of novel antibiotic substitute, pollution such as have no side effect because of having, noresidue is polluted, do not develop immunity to drugs, have disease-resistant, cure the disease, plurality of advantages such as diseases prevention, growth promotion, received the welcome of aquaculture.At present; Feeding micro-ecological preparation product on the market is more; Action effect mainly concentrates on aspects such as improving animal immunizing power, increase animal production capacity, has both had the feedstuff mildew effect, has the feed quality of improvement again; Improve the animal immunizing power effect, the product that increases animal production capacity but rarely has report.
Summary of the invention
The object of the present invention is to provide a kind of raising immunity of livestock that has, have the new microbial forage additive of feedstuff mildew function concurrently.
Another object of the present invention provides the preparation method and the premix that comprises this microbial forage additive of this microbial forage additive.
The objective of the invention is to realize through following technical scheme:
The present invention provides a kind of composite microorganism type forage additives; This additive is a kind of pulvis; It is mainly mixed by the composition of following weight percentage: bacillus pumilus (Bacillus pumilus) bacterium powder 30-50%, lactobacillus acidophilus (Lactobacillus acidophilus) bacterium powder 20-30%, saccharomyces cerevisiae (saccharomyces cerevisiae) cell wall polysaccharides 0.5-1%; Inulin 10%-30%, stone flour 1-10%.
Further; This additive is mainly mixed by the composition of following weight percentage: bacillus pumilus (Bacillus pumilus) bacterium powder 42%; Lactobacillus acidophilus (Lactobacillus acidophilus) bacterium powder 28%; Saccharomyces cerevisiae (saccharomyces cerevisiae) cell wall polysaccharides 1%, inulin 19%, stone flour 10%.
Wherein, Bacillus pumilus bacterium powder according to the invention, lactobacillus acidophilus bacterium powder, brewing yeast cell wall polysaccharide can be selected disclosed bacterial strain preparation in the prior art for use; But for reaching best effects of the present invention; The present invention preferably uses the bacillus pumilus of deposit number as CGMCC NO.4756, and deposit number is that the lactobacillus acidophilus of CGMCC No.5093 and the saccharomyces cerevisiae of the CGMCC No.2388 that deposit number is prepare bacterium powder or cell wall polysaccharides.
Wherein, described bacillus pumilus bacterium powder is dried under 40-90 ℃ of condition and is formed, and viable count is 5 * 10
8More than the CFU/g; Said lactobacillus acidophilus bacterium powder adopts vacuum freezing drying method, cryogenic temperature-55 ℃～-30 ℃, is prepared under vacuum 20～60Pa condition, and viable count is 2 * 10
7More than the CFU/g; In the said brewing yeast cell wall polysaccharide, gross protein value≤35%, beta glucan content>=30%, manna oligosacchride content>=20%; Inulin is a food grade materials; Stone flour is a carrier.
Wherein, the concrete preparation method of composite microorganism type forage additives of the present invention is following:
The preparation of A, lactobacillus acidophilus bacterium powder: lactobacillus acidophilus is inoculated in the MRS culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 2 * 10
9CFU/ml, bacterium liquid by volume: protective agent is that 1: 10 proportioning adds protective agent (the protective agent prescription is a skimmed milk 3%, trehalose 2%; Manganese sulfate 0.5%, sucrose 0.2%, other is a water); Behind-80 ℃ of pre-freeze 4h; Adopt vacuum freezing drying method, in cryogenic temperature-55～-30 ℃, process viable count greater than 2 * 10 under the vacuum 20-60Pa
7CFU/g lactobacillus acidophilus bacterium powder.
The preparation of B, bacillus pumilus bacterium powder: bacillus pumilus is inoculated in the LB culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 5 * 10
11CFU/ml, by weight bacterium liquid: carrier is that 1: 1 proportioning adds the carrier maize cob meal, and 60 ℃ of oven dry 24h process viable count greater than 5 * 10
8CFU/g bacillus pumilus bacterium powder.
The preparation of C, brewing yeast cell wall polysaccharide: the 1) preparation of S. cervisiae powder, the preparation method with reference to applicant's application number is: 200810106520.5 Chinese invention patent, get S. cervisiae powder 1000g; Add 5L sodium hydroxide solution (1mol/L); At 100 ℃ of following heating hydrolysis 4h, wait be cooled to room temperature after, 1000 * g; Centrifugal 10min carries out Separation of Solid and Liquid; 2) after the abandoning supernatant, add 1L water in the residue insoluble matter, stir, centrifugal again (1000 * g 10min), so repeats twice; 3) in insoluble matter, add the 1.5L acetum (10%, V/V) stir, in 85 ℃ of water-baths, be incubated 2h then; 4) like method 1) said method is carried out Separation of Solid and Liquid, and washed twice; 5) residue insoluble matter with washing with acetone three times after, drying obtains the yeast cell wall polyose: gross protein value≤35%, beta glucan content>=30%, the brewing yeast cell wall polysaccharide of manna oligosacchride content>=20%.
D, take by weighing each composition raw material by proportioning, raw material is put into mixer, wherein, the preferred mixture homogeneity coefficient of variation of mixer mixes less than 5% double-shaft paddle formula high efficient mixed machine, promptly gets.
The present invention also provides a kind of premix that comprises this composite microorganism type forage additives, and microbial forage additive adds percentage by weight in premix be 1-10 ‰.
Bacillus pumilus of the present invention (Bacillus pumilus) strain that is separated at home first for the applicant derives from the new bacillus pumilus of animal alimentary canal; From the cud of ox, separate a bacillus dominant strain that obtains for the applicant; Through Physiology and biochemistry and 16s RNA Analysis and Identification; Show this Pseudomonas in bacillus pumilus, its called after bacillus pumilus (Bacillus pumilus) of classifying was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 8th, 2011; Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica is called for short CGMCC, and deposit number is CGMCC No.4756.Saccharomyces cerevisiae among the present invention (Saccharomyces cerevisiae) has anti-adversity such as good stomach juice-resistant, bile tolerance, high temperature resistant, tolerance common antibiotics and produces acid, produces enzyme, suppresses benefit such as pathogen and give birth to functions; For the applicant separates from healthy animal enteron aisle or ight soil, seed selection obtains; Identify through Chinese agriculture microorganism fungus kind preservation administrative center; And being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on March 3rd, 2008, preserving number is that (the preservation proof is CGMCC No.2388: 200810106520.5 Chinese invention patent) referring to applicant's application number.Lactobacillus acidophilus of the present invention (Lactobacillus acidophilus) obtains for the applicant separates from animal intestinal; Can secrete antibiotin class material (acidolin, plain, the lactein of bacillus acidophilus); Antagonism to the pathogenic entero becteria generation; The propagation that suppresses the enteron aisle undesirable microorganism, adjustment gut flora balance, the applicant has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 26th, 2011; Preservation address and be called for short the samely, preserving number is CGMCCNo.5093.
Beneficial effect of the present invention is: the strain that used bacillus pumilus CGMCC NO.4756 is separated to for the applicant first at home among the present invention derives from the new bacillus pumilus of animal alimentary canal; And it is applied in the animal premix as feed addictive, first Application is in the Midew preventive for feed aspect simultaneously; And this bacterial strain has resistant to elevated temperatures characteristics, thereby does not have and need encapsulate, the advantage that can directly granulate; Bacillus pumilus of the present invention is added in the premix as a kind of feed addictive, can improve feed quality, improves the content of crude protein in the feed; Reduce coarse-fibred content in the feed; More conventional mould inhibitor does not contain chemical agent except safety, pollution-free, direct granulation simultaneously, and not only anti-mold effect is good; Antibacterial power is strong, and can improve feed quality; Yeast cell wall polysaccharide among the present invention is to be prepared from through the alkali preparation method S. cervisiae powder CGMCC No.2388; This product is rich in the biological immune factors such as beta glucan and functional oligosaccharides; Can significantly strengthen cytophagous activity and phagocytic activity, strengthen the function of animal immune system, improve microbial environment in the animal body; Identification adheres to and eliminates mycotoxin in the feed, improves food conversion ratio.The used lactobacillus acidophilus CGMCC of the present invention No.5093 has stronger acid producing ability; The adding of lactobacillus acidophilus not only can be created slightly acidic environment, strengthens the stability of mould inhibitor; Also can give feed good organoleptic properties; Strengthening the animal intestinal resistance, prevention and treatment diarrhoea, promoting feed safety property aspect has good benefit to give birth to effect.
Probiotics of the present invention is by bacillus pumilus, lactobacillus acidophilus, yeast cell wall polysaccharide and inulin is composite forms, and palatability is strong, not only has the reinforcement animal immune system; Selectivity promotes animal intestinal beneficial bacterium propagation; The field planting that suppresses beneficial bacterium with breed, improve microbial environment in the animal body, the stimulation immune response; Strengthen immunity of livestock, improve livestock and poultry production performance; Reduce antibiotic and use, mycotoxins in feed is had good identification adhesion and elimination ability, the function of improving food conversion ratio; Can be used as simultaneously a kind of Midew preventive for feed again; It is few also to have addition, and preparation technology is simple, has no side effect, and advantages such as noresidue have higher market value.
The specific embodiment
Embodiment 1: the preparation of composite microorganism type forage additives
The preparation of A, lactobacillus acidophilus bacterium powder: lactobacillus acidophilus CGMCC NO.5093 is inoculated in the MRS culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 2 * 10
9CFU/ml, by bacterium liquid: 1: 10 proportioning of protective agent (volume ratio) adds protective agent, and (the protective agent prescription is a skimmed milk 3%, trehalose 2%; Manganese sulfate 0.5%, sucrose 0.2%, other is a water); Behind-80 ℃ of pre-freeze 4h; Adopt vacuum freezing drying method, in cryogenic temperature-50 ℃, processing viable count under the vacuum 30Pa is 2 * 10
8CFU/g lactobacillus acidophilus bacterium powder.
The preparation of B, bacillus pumilus bacterium powder: bacillus pumilus CGMCC NO.4756 is inoculated in the LB culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 5 * 10
11CFU/ml, press bacterium liquid: 1: 1 proportioning of carrier (weight ratio) adds the carrier maize cob meal, 60 ℃ of oven dry 24h, processing viable count is 5 * 10
9CFU/g bacillus pumilus bacterium powder.
The preparation of C, brewing yeast cell wall polysaccharide: 1) get S. cervisiae powder 1000g, add 5L sodium hydroxide solution (1mol/L), at 100 ℃ of following heating hydrolysis 4h, wait be cooled to room temperature after, it is centrifugal that (1000 * g 10min), carries out Separation of Solid and Liquid; 2) after the abandoning supernatant, add 1L water in the residue insoluble matter, stir, centrifugal again (1000 * g 10min), so repeats twice; 3) in insoluble matter, add the 1.5L acetum (10%, V/V) stir, in 85 ℃ of water-baths, be incubated 2h then; 4) like method 1) said method is carried out Separation of Solid and Liquid, and washed twice; 5) residue insoluble matter with washing with acetone three times after, drying obtains the yeast cell wall polyose: gross protein value is 35%, beta glucan content is 30%, manna oligosacchride content is 20% brewing yeast cell wall polysaccharide.
D, take by weighing each composition raw material by proportioning, proportioning is following: bacillus pumilus bacterium powder 35%, lactobacillus acidophilus bacterium powder 30%, brewing yeast cell wall polysaccharide 0.5%, inulin 29.5%, stone flour 5%; Raw material is put into the mixture homogeneity coefficient of variation less than 5% double-shaft paddle formula high efficient mixed machine, mix, promptly get.
Embodiment 2: the preparation of composite microorganism type forage additives
The preparation of A, lactobacillus acidophilus bacterium powder: lactobacillus acidophilus CGMCC NO.5093 is inoculated in the MRS culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 2 * 10
9CFU/ml, by bacterium liquid: 1: 10 proportioning of protective agent (volume ratio) adds protective agent, and (the protective agent prescription is a skimmed milk 3%, trehalose 2%; Manganese sulfate 0.5%, sucrose 0.2%, other is a water); Behind-80 ℃ of pre-freeze 4h; Adopt vacuum freezing drying method,, process viable count 5 * 10 under the vacuum 30Pa in cryogenic temperature-50 ℃
8CFU/g lactobacillus acidophilus bacterium powder.
The preparation of B, bacillus pumilus bacterium powder: bacillus pumilus CGMCC NO.4756 is inoculated in the LB culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 5 * 10
11CFU/ml, press bacterium liquid: 1: 1 proportioning of carrier (weight ratio) adds the carrier maize cob meal, and 60 ℃ of oven dry 24h process viable count greater than 8 * 10
9CFU/g bacillus pumilus bacterium powder.
The preparation of C, brewing yeast cell wall polysaccharide: 1) get S. cervisiae powder 1000g, add 5L sodium hydroxide solution (1mol/L), at 100 ℃ of following heating hydrolysis 4h, wait be cooled to room temperature after, it is centrifugal that (1000 * g 10min), carries out Separation of Solid and Liquid; 2) after the abandoning supernatant, add 1L water in the residue insoluble matter, stir, centrifugal again (1000 * g 10min), so repeats twice; 3) in insoluble matter, add the 1.5L acetum (10%, V/V) stir, in 85 ℃ of water-baths, be incubated 2h then; 4) like method 1) said method is carried out Separation of Solid and Liquid, and washed twice; 5) residue insoluble matter with washing with acetone three times after, drying, obtaining yeast cell wall polyose gross protein value is 30%, beta glucan content is 45%, manna oligosacchride content is 30% brewing yeast cell wall polysaccharide.
D, take by weighing each composition raw material by proportioning, proportioning is following: bacillus pumilus bacterium powder 42%, lactobacillus acidophilus bacterium powder 28%, brewing yeast cell wall polysaccharide 1%, inulin 19%, stone flour 10%; Raw material is put into the mixture homogeneity coefficient of variation less than 5% double-shaft paddle formula high efficient mixed machine, mix, promptly get.
Embodiment 3: the preparation of composite microorganism type forage additives
The preparation of A, lactobacillus acidophilus bacterium powder: lactobacillus acidophilus CGMCC NO.5093 is inoculated in the MRS culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 2 * 10
9CFU/ml, by bacterium liquid: 1: 10 proportioning of protective agent (volume ratio) adds protective agent, and (the protective agent prescription is a skimmed milk 3%, trehalose 2%; Manganese sulfate 0.5%, sucrose 0.2%, other is a water); Behind-80 ℃ of pre-freeze 4h; Adopt vacuum freezing drying method, in cryogenic temperature-50 ℃, processing viable count under the vacuum 30Pa is 6 * 10
8CFU/g lactobacillus acidophilus bacterium powder.
The preparation of B, bacillus pumilus bacterium powder: bacillus pumilus CGMCC NO.4756 is inoculated in the LB culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 5 * 10
11CFU/ml, press bacterium liquid: 1: 1 proportioning of carrier (weight ratio) adds the carrier maize cob meal, and 60 ℃ of oven dry 24h process viable count greater than 4 * 10
9CFU/g bacillus pumilus bacterium powder.
The preparation of C, brewing yeast cell wall polysaccharide: 1) get S. cervisiae powder 1000g, add 5L sodium hydroxide solution (1mol/L), at 100 ℃ of following heating hydrolysis 4h, wait be cooled to room temperature after, it is centrifugal that (1000 * g 10min), carries out Separation of Solid and Liquid; 2) after the abandoning supernatant, add 1L water in the residue insoluble matter, stir, centrifugal again (1000 * g 10min), so repeats twice; 3) in insoluble matter, add the 1.5L acetum (10%, V/V) stir, in 85 ℃ of water-baths, be incubated 2h then; 4) like method 1) said method is carried out Separation of Solid and Liquid, and washed twice; 5) residue insoluble matter with washing with acetone three times after, drying, obtaining yeast cell wall polyose gross protein value is 25%, beta glucan content is 35%, manna oligosacchride content is 31% brewing yeast cell wall polysaccharide.
D, take by weighing each composition raw material by proportioning, proportioning is following: bacillus pumilus bacterium powder 50%, lactobacillus acidophilus bacterium powder 22%, brewing yeast cell wall polysaccharide 1%, inulin 17%, stone flour 10%; Raw material is put into the mixture homogeneity coefficient of variation less than 5% double-shaft paddle formula high efficient mixed machine, mix, promptly get.
Embodiment 4, the application of composite microorganism type forage additives aspect the weanling pig breed
Selecting 144 average weights for use is the white two-way cross weanling pig of Da Bai * length of 8.65 ± 0.42kg, and the wean age in days is 28 ± 2d, and the principle not consistent according to its body weight, sex, nest is divided into 4 processing at random; The basal feed (blank group) of feeding respectively adds the feed addictive of embodiment 2 preparations in the basal feed, adding proportion is 0.5 ‰ (testing 1 group); 5 ‰ (testing 2 groups); The daily ration of 10 ‰ (testing 3 groups), 6 repetitions of every processing, each repeats 6 pigs; Public mother half and half, experimental period is 35d.Experimental result such as table 1.
The result shows; The equal feed consumption rate of experimental group average net gain in latter stage, daily gain and head that adds compound micro-ecological preparation all is higher than the blank group; This shows; The interpolation of this feeding micro-ecological preparation has the growth performance of effective raising weanling pig, reduces feedstuff-meat ratio, reduces the effect of the diarrhea rate of piglet.Analysis-by-synthesis draws, and the preferred addition of compound micro-ecological preparation is 5 ‰, and at this moment, experimental group is compared with the blank group, and piglet average net gain in latter stage improves 33.7%, and average daily weightening finish improves 33.5%, and the material anharmonic ratio significantly is lower than control group, and the grice diarrhoea rate is 0.
Table 1 various dose probiotics is to the influence of weanling pig growth performance
Annotate: indicate same letter person with line of numbers and represent difference not remarkable (p＞0.05), different alphabetical persons represent significant difference (p＜0.05).
Embodiment 5: add compound micro-ecological preparation in the daily ration that goes mouldy to meat chick Immune Effects
Select 450 of 7 age in days healthy AA meat chicks for use, be divided into 5 groups at random by body weight, every group of 6 repetitions, each repeats 15 chickens.Grouping situation such as table 2.
Liver is the target organ of aflatoxin effect, but is not unique impaired organ.The meat chick is searched for food behind the aflatoxin, at first hepatic injury can occur, and searching for food for a long time also to cause other organ to receive damage in various degree.The corn that goes mouldy can significantly improve the relative weight of fryer liver, heart, kidney, spleen.In addition, aflatoxin can bring out immunosupress, makes the immune organ impaired development, causes the spleen enlargement, the bursa of farbricius, atrophy of thymus gland.
Table 2 experimental design
| Group | Daily ration is formed |
| Control group | Normal corn |
| The moldy feed group | Normal corn in the daily ration is substituted by fermented maize |
| Test group I | The compound micro-ecological preparation of moldy feed+0.5 ‰ embodiment, 1 preparation |
| Test group II | The compound micro-ecological preparation of moldy feed+5 ‰ embodiment, 1 preparation |
| Test group III | The compound micro-ecological preparation of moldy feed+10 ‰ embodiment, 1 preparation |
The research of this experiment shows, the obvious swelling when test 7d and 21d of moldy feed group liver, and obvious enlargement also appears in spleen during 7d, and atrophy appears in the thymus gland and the bursa of farbricius.Compare with the moldy feed group, the swelling of fryer internal organs and the atrophy of immune organ phenomenon of test group have obtained alleviation, add 10 ‰ probiotics groups and have significantly improved the relative weight of the bursa of farbricius, and make it be higher than the control group level.Therefore, compound micro-ecological preparation of the present invention can reduce the feed that goes mouldy to a certain extent to the toxic action of internal organ, and has promoted the growth of immune organ.Shown in experimental result such as the table 3, table 4.
Table 3 compound micro-ecological preparation is to the influence (g/kg) of meat chick immune organ weight
Annotate: indicate same letter person with line of numbers and represent difference not remarkable (p＞0.05), different alphabetical persons represent significant difference (p＜0.05).
Table 4 compound micro-ecological preparation is to the influence (g/kg) of meat chick immune organ relative weight
Annotate: indicate same letter person with line of numbers and represent difference not remarkable (p＞0.05), different alphabetical persons represent significant difference (p＜0.05).
Embodiment 6: the application of compound micro-ecological preparation aspect the mildew-resistant of growing and fattening pigs complete diet pellet
Fresh pig complete diet pellet 30kg is divided into three groups, and control group does not add mould inhibitor, and test group I adds 50% calcium propionate Midew preventive for feed by 1 ‰ addition, and test group II adds the compound micro-ecological preparation of the embodiment of the invention 2 preparations by 5 ‰ addition.According to aspergillus flavus optimum growing condition in the bibliographical information; To test feed and leave 30 ℃ in, humidity is to preserve in 75% the incubator, and every 10d gathers the feed sample; Test period is 60d; Get 20g for each every group, detect aflatoxin in the feed with the ELISA method, experimental result is as shown in table 5.
Table 5 compound micro-ecological preparation is to the anti-mold effect of growing and fattening pigs complete feed
Research shows; Experiment 60d, control group feed aflatoxin content is up to 76.18 μ g/kg, and test group I feed aflatoxin content is 15.18 μ g/kg; Aflatoxin content is 14.14 μ g/kg in the test group II feed, is lower than 20 μ g/kg of national forage health standard.Therefore, even in the optimum condition of aspergillus flavus growth and breeding, in feed, add the generation that 5 ‰ compound micro-ecological preparations can effectively suppress mycotoxin in the feed, the calcium propionate class mould inhibitor effect of using always in its anti-mold effect and the feed is basic identical.
Claims (6)
1. composite microorganism type forage additives; It is characterized in that: it is a kind of pulvis; This additive is mainly mixed by the composition of following weight percentage: bacillus pumilus (Bacillus pumilus) bacterium powder 30-50%, lactobacillus acidophilus (Lactobacillus acidophilus) bacterium powder 20-30%, saccharomyces cerevisiae (saccharomyces cerevisiae) cell wall polysaccharides 0.5-1%; Inulin 10%-30%, stone flour 1-10%.
2. like power 1 described composite microorganism type forage additives; It is characterized in that: this additive is mainly mixed by the composition of following weight percentage: bacillus pumilus (Bacillus pumilus) bacterium powder 42%; Lactobacillus acidophilus (Lactobacillus acidophilus) bacterium powder 28%; Saccharomyces cerevisiae (saccharomyces cerevisiae) cell wall polysaccharides 1%, inulin 19%, stone flour 10%.
3. like power 1 described composite microorganism type forage additives, it is characterized in that: the deposit number of said bacillus pumilus is CGMCC NO.4756, and the deposit number of said lactobacillus acidophilus is CGMCCNo.5093; The deposit number of said saccharomyces cerevisiae is CGMCC No.2388.
4. like power 1 described composite microorganism type forage additives, it is characterized in that: described bacillus pumilus bacterium powder is dried under 40-90 ℃ of condition and is formed, and viable count is 5 * 10
8More than the CFU/g; Said lactobacillus acidophilus bacterium powder adopts vacuum freezing drying method, cryogenic temperature-55 ℃～-30 ℃, is prepared under vacuum 20～60Pa condition, and viable count is 2 * 10
7More than the CFU/g; In the said brewing yeast cell wall polysaccharide, gross protein value≤35%, beta glucan content>=30%, manna oligosacchride content>=20%.
5. like the preparation method of each described composite microorganism type forage additives of power 1-4, it is characterized in that: comprise the steps:
The preparation of A, lactobacillus acidophilus bacterium powder: lactobacillus acidophilus is inoculated in the MRS culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 2 * 10
9CFU/ml, bacterium liquid by volume: protective agent is that 1: 10 proportioning adds protective agent, and the protective agent prescription is following: skimmed milk 3%, and trehalose 2%, manganese sulfate 0.5%, sucrose 0.2%, other is a water; Adopt vacuum freezing drying method, in cryogenic temperature-55～-30 ℃, process viable count greater than 2 * 10 under the vacuum 20-60Pa
7CFU/g lactobacillus acidophilus bacterium powder;
The preparation of B, bacillus pumilus bacterium powder: bacillus pumilus is inoculated in the LB culture medium, 37 ℃ of static cultivation 12h, adjustment bacteria concentration to 5 * 10
11CFU/ml, by weight bacterium liquid: carrier is that 1: 10 proportioning adds the carrier maize cob meal, and 60 ℃ of oven dry 24h process viable count greater than 5 * 10
8CFU/g bacillus pumilus bacterium powder;
The preparation of C, brewing yeast cell wall polysaccharide: 1) preparation S. cervisiae powder, get S. cervisiae powder 1000g, adding 5L concentration is the sodium hydroxide solution of 1mol/L; At 100 ℃ of following heating hydrolysis 4h, wait be cooled to room temperature after, 1000 * g; Centrifugal 10min carries out Separation of Solid and Liquid; 2) after the abandoning supernatant, add 1L water in the residue insoluble matter, stir, centrifugal 10min under 1000 * g repeats twice; 3) in insoluble matter, adding the 1.5L volume ratio is 10% acetum, stirs, and in 85 ℃ of water-baths, is incubated 2h then; 4) like method 1) said method is carried out Separation of Solid and Liquid, and washed twice; 5) residue insoluble matter with washing with acetone three times after, drying obtains the yeast cell wall polyose: gross protein value≤35%, beta glucan content>=30%, the brewing yeast cell wall polysaccharide of manna oligosacchride content>=20%;
D, take by weighing each composition raw material, raw material is put into mixer, mix, promptly get by proportioning.
6. premix that comprises each described composite microorganism type forage additives of power 1-4, it is characterized in that: the percentage by weight that composite microorganism type forage additives adds in premix is 1-10 ‰.
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| CN102885206A (en) * | 2012-10-29 | 2013-01-23 | 曾伟 | Natural animal feed additive |
| CN102925374A (en) * | 2012-11-01 | 2013-02-13 | 北京大北农科技集团股份有限公司 | New saccharomyces cerevisiae strain and feed additive and batch containing same |
| CN102940145A (en) * | 2012-11-29 | 2013-02-27 | 广东希普生物科技股份有限公司 | Preparation method of fermentation aquatic health care feed |
| CN103243028A (en) * | 2012-02-02 | 2013-08-14 | 安琪酵母股份有限公司 | Yeast cell wall and preparation method thereof, forage additive and preparation method thereof, and forage |
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| CN102559539A (en) * | 2011-12-02 | 2012-07-11 | 北京大北农科技集团股份有限公司 | Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof |
| CN102559539B (en) * | 2011-12-02 | 2015-04-15 | 北京大北农科技集团股份有限公司 | Lactobacillus acidophilus and application thereof and feed additive thereof and premix compound thereof |
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| CN103740624B (en) * | 2014-01-17 | 2015-06-17 | 威海光洋生物科技有限公司 | Bacillus pumilus and application thereof to preparation of exopolysaccharides |
| CN105746850A (en) * | 2014-12-16 | 2016-07-13 | 北京大北农科技集团股份有限公司 | Low-temperature fermentation feed raw material and application of same in preparation of mixed feed for pig |
| CN105595030A (en) * | 2016-03-24 | 2016-05-25 | 江苏盐城源耀生物科技有限公司 | Biologically prepared mildew preventive and preparation process thereof |
| CN106538824A (en) * | 2016-09-26 | 2017-03-29 | 广州好师傅生物科技有限公司 | A kind of polypeptide type feed addictive and preparation method thereof |
| CN106615779A (en) * | 2016-11-16 | 2017-05-10 | 山东众客食品有限公司 | Mycotoxin-resistant feed additive for pigs, as well as preparation method and application of mycotoxin-resistant feed additive |
| CN108200999B (en) * | 2016-12-19 | 2022-02-08 | 安琪酵母股份有限公司 | Yeast composition for ruminants, feed, preparation method and application |
| CN108200999A (en) * | 2016-12-19 | 2018-06-26 | 安琪酵母股份有限公司 | Ruminant yeast compositions and feed, preparation method and application |
| CN106901137A (en) * | 2016-12-31 | 2017-06-30 | 安徽宏亮饲料科技有限公司 | A kind of detoxicating mycotoxin feed addictive |
| CN107439795A (en) * | 2017-06-29 | 2017-12-08 | 青岛旺海源生物科技有限公司 | A kind of feed addictive and its preparation technology containing compound marine alga |
| CN109619267A (en) * | 2017-10-09 | 2019-04-16 | 北京大北农科技集团股份有限公司 | A kind of feeding mold toxin sorbent and the preparation method and application thereof |
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| CN108813178B (en) * | 2018-04-20 | 2022-04-05 | 广东壹号地方猪研究院有限公司 | Application of fermented fruit residues in improving pig immunity and visceral organ development |
| CN108576383A (en) * | 2018-05-25 | 2018-09-28 | 河南牧鹤(集团)饲料有限公司 | A kind of chicken feed and its processing method |
| CN112155120A (en) * | 2020-09-30 | 2021-01-01 | 河北科技大学 | Micro-ecological synbiotic preparation for feed, preparation method and application |
| CN112931706A (en) * | 2021-02-08 | 2021-06-11 | 宁夏大洋饲料科技有限公司 | Special compound premix feed for dairy cows in early perinatal period and preparation method thereof |
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Application publication date: 20120125 Assignee: JIANGXI GAOAN DABEINONG FORAGE CO., LTD. Assignor: Zhangzhou agricultural farming and animal husbandry science and Technology Co., Ltd.|Beijing DaBeiNong Technology Group Limited by Share Ltd|Inner Mongolia sijichun Feed Co. Ltd. Contract record no.: 2015990000032 Denomination of invention: Composite micro-ecological feed additive and preparation method and premix thereof Granted publication date: 20131016 License type: Exclusive License Record date: 20150119 |
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Assignee: JIANGXI GAOAN DABEINONG FORAGE CO., LTD. Assignor: Zhangzhou agricultural farming and animal husbandry science and Technology Co., Ltd. |Beijing DaBeiNong Technology Group Limited by Share Ltd| Inner Mongolia sijichun Feed Co. Ltd. Contract record no.: 2015990000032 Date of cancellation: 20160115 |
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Effective date of registration: 20160129 Address after: 100080 Beijing City, Haidian District Zhongguancun street, No. 27 Zhongguancun building 14 DaBeiNong group Patentee after: Beijing Dabeinong Technology Group Co., Ltd. Patentee after: Inner Mongolia Ever Spring Feed Co., Ltd. Patentee after: Zhangzhou Dabeinong Agriculture & Pasture Technology Co., Ltd. Patentee after: JIANGXI GAOAN DABEINONG FORAGE CO., LTD. Address before: 100080 Beijing City, Haidian District Zhongguancun street, No. 27 Zhongguancun building 14 DaBeiNong group Patentee before: Beijing Dabeinong Technology Group Co., Ltd. Patentee before: Inner Mongolia Ever Spring Feed Co., Ltd. Patentee before: Zhangzhou Dabeinong Agriculture & Pasture Technology Co., Ltd. |
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Effective date of registration: 20160429 Address after: 100080 Beijing City, Haidian District Zhongguancun street, No. 27 Zhongguancun building 14 DaBeiNong group Patentee after: Beijing Dabeinong Technology Group Co., Ltd. Patentee after: JIANGXI GAOAN DABEINONG FORAGE CO., LTD. Address before: 100080 Beijing City, Haidian District Zhongguancun street, No. 27 Zhongguancun building 14 DaBeiNong group Patentee before: Beijing Dabeinong Technology Group Co., Ltd. Patentee before: Inner Mongolia Ever Spring Feed Co., Ltd. Patentee before: Zhangzhou Dabeinong Agriculture & Pasture Technology Co., Ltd. Patentee before: JIANGXI GAOAN DABEINONG FORAGE CO., LTD. |