CN102320708B - Method for degrading synthetic dye through compound system - Google Patents
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Abstract
The invention discloses a method for degrading a synthetic dye through a compound system, which belongs to the field of wastewater processing. The method has the following processing steps of: (1) adding printing and dyeing wastewater into a reactor, wherein the chrominance of the printing and dyeing wastewater to be processed is equal to or lower than 50000 times of chrominance; (2) respectively and sequentially adding zero-valent iron, a white-rot fungus film and an acid-secreting bacterium film into the printing and dyeing wastewater; and (3) carrying out decoloring processing after adding the processing materials, reacting under natural conditions at the temperature of 20 to 40 DEG C and under a normal pressure, and stirring once at intervals of 5 to 7 hours until the decoloring rate and the chrominance are kept stable. Through the method, the advantages of individually processing the zero-valent iron and white-rot fungi are integrated, the advantages of higher decoloring rate and lower processing-fluid toxicity are represented, the acting strength of the zero-valent iron and the white-rot fungi can be enhanced through the introduction of acid-secreting bacteria, synthetic dye wastewater can be processed with high efficiency, the decoloring rate reaches more than 85 percent, and compared with a method for individually processing the white-rot fungi, the processing time is shortened by about 43 percent.
Description
Technical field
The invention belongs to field of waste water treatment, be specifically related to a kind of method that compound system that adopts iron powder, white-rot fungi mycoderm and secrete sour bacterium mycoderm carrys out synthetic dyestuff in efficient degradation waste water from dyestuff.
Background technology
Synthetic dyestuff are weavings, leather, printing, the common water pollutants of industry trade effluent such as medical treatment, complicated components, in waste water, contain a large amount of organism and salt, there is high COD, the features such as color and luster is dark, in waste water from dyestuff, contain various types of synthetic dyestuff, as Anthraquinones, azo, triphenylmethane, the dyestuffs such as phthalocyanines, these dyestuff great majority have carcinogenic, teratogenesis, the harm such as mutagenesis, when these dyestuffs are discharged in water body, can jeopardize hydrobiont, to liver, the gill, kidney, intestines, sexual gland, pituicytes etc. produce deleterious effect, therefore the removal of dyestuff just becomes one of major objective of dye wastewater treatment.
The method that the dyestuff having adopted is at present removed comprises that Physical, chemical method and biological process process, as means such as ion-exchange, cohesion, membrane filtration, ozonize, absorption, Zero-valent Iron, white rot fungus degradings, wherein Zero-valent Iron decolouring, white rot fungus degrading are more effective two kinds of methods.Iron is active metal, there is reducing power, in acidic aqueous solution, can directly reducing dyes be become to amino organism, because amino is organic, look for lightly, and easy oxidized decomposition, therefore can make chroma in waste water be minimized (Yan Jinxia, Dong Shaofeng etc., the research of Zero-valence Iron Process dye wastewater treatment using decolorizing effect, dyestuff and dyeing, 2007).Zero-valent Iron decolorization rate is fast, even in tens of minutes, percent of decolourization just can reach more than 80%, but there is the shortcoming (Wang Wenjun that cannot remove up hill and dale pollutent, Ruan Hongquan etc., Congo red Zero-valent Iron reduction-decolor condition and mechanism research, environmental science and management, 2008), simultaneous reactions must carry out in low pH environment, in order to avoid form passivation layer (Tang Xinhu at iron surface, Huang Lisha etc., ultrasonic synergistic Zero-valent Iron degrading activity bright red X-3B, environmental science, 2006), but because most waste water from dyestuff present alkalescence (Joe MH, Lim SY et al., Decolorization of reactive dyes by
clostridium bifermentanssL186 isolated from contaminated soil, World J Microbiol Biotechnol, 2008), while processing waste water, to reduce the pH of waste water from dyestuff, can increase cost like this, also can cause secondary pollution problems simultaneously.White rot fungus degrading method has the advantage that thorough degradation of dye becomes carbonic acid gas and water, but has longer shortcoming of decolouring cycle, generally wants time a couple of days; Most white-rot fungis just demonstrate higher efficiency (Ali N, Ikramullah L G, et al., Decolorization of Acid red 151 by within the scope of low pH simultaneously
aspergillus nigersA1 under different physicochemical conditions, World J Microbiol Biotechnol, 2008; Mazmanci MA, Unyayar A, Decolorization efficiency of
funalia trogiiunder static condition:Effect of C:N ratios, Afr J Biotechnol, 2010; Rismayani S, Fukushima M, et al., Decolorization of orange II by catalytic oxidationusing iron (III) phthalocyanine-tetrasulfonic acid, J Hazard Mater, 2004).
In former research, due to the quick decolouring advantage of Zero-valent Iron, environmental research worker has given to show great attention to it, and pursues simply the agility of decolouring, and ignored, processes the variation of product and the impact that may cause environment thereof; And prefer on the other hand, use the white-rot fungi research worker who processes of decolour when being applied to decolour, to pay close attention to more the metabotic change of dyestuff, more focus on the protection to environment, and be less concerned about the agility of processing; Two kinds of methods all need to maintain the better treatment effect of low pH competence exertion when applying separately simultaneously, as a rule because use hydrochloric acid is more convenient and cost is not very high, research worker often preferentially selects hydrochloric acid to reduce the pH of waste water from dyestuff, up to the present, not yet adopt the relative biogenic organic acid slowly of release rate to reduce the pH of dye wastewater treatment system.
Summary of the invention
the technical problem that invention will solve
The Zero-valent Iron decoloring method using in dye wastewater treatment process at present has higher efficiency, but decolouring is not thorough, and need to maintain low pH environment; White-rot fungi decolouring is comparatively thorough, but the cycle is long, and majority just shows higher efficiency within the scope of low pH.For overcoming the shortcoming of above-mentioned two kinds of methods, the present invention is incorporated into degraded system by above-mentioned two kinds of methods simultaneously, by secreting sour bacterium, also introduce this system to maintain the solution environmental of low pH simultaneously, the present invention's product after Zero-valent Iron reduction of can better degrading, can be conducive to the reducing power of Zero-valent Iron and the degradation capability of white-rot fungi more.
technical scheme
Technical scheme of the present invention is: the method for a kind of degrading synthetic dye through compound system of the present invention, the steps include:
(1) dyeing waste water is joined in reactor, the colourity of pending dyeing waste water is equal to or less than 50000 times of colourities;
(2) respectively by Zero-valent Iron, white-rot fungi mycoderm with secrete sour bacterium mycoderm and successively add in dyeing waste water;
(3) add after above-mentioned processing material, the processing of decolouring, stirred once every 5 ~ 7 hours, until percent of decolourization and colourity keep stable.
The reaction that in step (3), decolouring is processed is carried out under field conditions (factors), and temperature is 20 ~ 40 ℃, and pressure is normal pressure.
Zero-valent Iron described in step (2) is iron powder, and iron powder add-on is 0.5% ~ 1.5% of waste water weight; Described white-rot fungi mycoderm add-on is 7% ~ 9% of waste water weight, and wherein dry mycelium content is 1.5% ~ 2.5%, and described white-rot fungi is Irpex lacteus; Described to secrete sour bacterium mycoderm add-on be 7% ~ 9% of waste water weight, and wherein dry mycelium content is 1.5% ~ 2.5%, and the described sour bacterium that secretes is aspergillus niger XZ-127, and the described sour bacterium pH value of fermented liquid in substratum of secreting is less than 4.Wherein, best adding proportion is: described iron powder add-on is 1% of waste water weight; Described white-rot fungi mycoderm add-on is 8% of waste water weight; It is described that to secrete sour bacterium mycoderm add-on be 8% of waste water weight.
The cultural method of described Irpex lacteus is that the Irpex lacteus of preservation is inoculated in potato liquid nutrient medium is housed, the leach liquor and the glucose that in substratum, comprise potato, under field conditions (factors), cultivate and form mycelium pellet after 4 ~ 6 days, under sterile state, be broken into bacteria suspension, draw bacteria suspension and put to being equipped with in the culture dish of sterilizing potato liquid nutrient medium, under field conditions (factors), cultivate and form mycoderm after 4 ~ 6 days, as the inoculum of dyestuff degraded.
beneficial effect
Compared with prior art, its remarkable advantage is in the present invention:
(1) a kind of method that the invention provides Zero-valent Iron, white-rot fungi and secrete synthetic dyestuff in sour bacterium composite degradation system dye wastewater treatment using, combine the advantage of Zero-valent Iron and white-rot fungi individual curing, show percent of decolourization higher, the advantage that treatment solution toxicity is lower, and the introducing of secreting sour bacterium can be strengthened the action intensity of Zero-valent Iron, white-rot fungi, can efficiently process synthetic dyestuff waste water, decolour more thorough, percent of decolourization reaches more than 85%, compare the method for white-rot fungi individual curing, the treatment time shortens approximately 43%;
(2) the present invention carries out at normal temperatures and pressures, and reaction conditions is simple;
(3) when waste water is processed in Zero-valent Iron decolouring, will reduce the pH of alkaline waste water, and the present invention will secrete sour bacterium introducing decolouring system, can effectively maintain decolouring system in low pH state of value, reduce cost input, and be difficult for forming secondary pollution.
Embodiment
Further illustrate by the following examples the present invention.
Embodiment 1
(1) comparative example: Zero-valent Iron is processed experiment to the independent decolouring of dyestuff.
The independent decolouring of Zero-valent Iron to malachite green 617nm, Viola crystallina 588nm, aniline blue 585 nm, orange G 475nm, thymol blue 376nm, methylene blue 668nm, tetrabromo-mcresolsulfonphthalein 618nm, tropeolin-D 505nm, methyl red 410nm: get zeroth order iron powder 1g and add to be equipped with in the Erlenmeyer flask of above-mentioned each dyestuff that 100ml concentration is 100mg/L and decolour, every 1 hour, measure its light absorption value, and calculate percent of decolourization, percent of decolourization (%)=[(A
0-A
t)/A
0] * 100%.
Wherein: A
tthe light absorption value of dyestuff during for time t, A
0for the initial light absorption value before dyestuff degraded.
Determination experiment result is as shown in table 1 below.
The decolouring of table 1 Zero-valent Iron to nine kinds of dyestuffs
| Testing index | Malachite green | Viola crystallina | Aniline blue | Orange G | Thymol blue | Methylene blue | Tetrabromo-mcresolsulfonphthalein | Tropeolin-D | Methyl red |
| Solution nature pH value | 6 | 6.5 | 7 | 7 | 6 | 7 | 7 | 6 | 7 |
| Decolour steady time (hour) | 4 | >10 | 2 | 8 | >10 | 5 | 8 | 7 | 8 |
| Percent of decolourization | 96% | 14%(the 10th hour) | 92% | 43% | 17%(the 10th hour) | 75% | 14% | 77% | 39% |
(2) comparative example: white-rot fungi mycoderm is processed experiment to the independent decolouring of dyestuff.
Irpex lacteus in white-rot fungi (
irpex lacteusxX-5) polytype dyestuff (Novotny C, the Biodegeradation of synthetic dyes by of can degrading that be in the news
irpex lacteusunder various growth conditions, International Biodeterioration and Biodegeradation, 2004), the validity of Irpex lacteus as example explanation the inventive method is take in the present invention.
The concrete cultural method of Irpex lacteus is as follows: the Irpex lacteus of preservation is inoculated in 50ml potato liquid nutrient medium (abbreviation PDB is housed, the leach liquor that comprises 200 grams of potatoes in every 1L substratum and 20 grams of glucose) 150ml triangular flask in cultivate after 5 days at 27 ℃ and form mycelium pellet, under sterile state, with aseptic glass stick, be broken into bacteria suspension, with pipettor, draw 1ml bacteria suspension in the culture dish of diameter 9cm that 20ml sterilizing potato liquid nutrient medium is housed, at 27 ℃, cultivate after 5 days and form mycoderm, as the inoculum of dyestuff degraded.Getting cultured white-rot fungi mycoderm 8g(dry mycelium content is in advance 2%), the Erlenmeyer flask that is placed in 100ml dye strength and is 100mg/L carries out dyestuff degraded.
Determination experiment result is as shown in table 2 below.
The decolouring of table 2 Irpex lacteus to nine kinds of dyestuffs
| Testing index | Malachite green | Viola crystallina | Aniline blue | Orange G | Thymol blue | Methylene blue | Tetrabromo-mcresolsulfonphthalein | Tropeolin-D | Methyl red |
| Solution nature pH value | 6 | 6.5 | 7 | 7 | 6 | 7 | 7 | 6 | 7 |
| Decolour steady time (my god) | 4 | >7 | 2 | 4 | 5 | 5 | 4 | 4 | 3 |
| Percent of decolourization | 87% | No bleaching | 93% | 78% | 65% | 79% | 63% | 89% | 91% |
(3) comparative example: secrete sour bacterium mycoderm the independent decolouring of dyestuff is processed to experiment.
With antagonistic experiment test white-rot fungi and secrete whether there is mutual restraining effect between sour bacterium.Be chosen in fermented liquid pH in PDB substratum lower than 4 fungi as secreting by force sour bacterium for the present invention.
It is 3 that its wave and culture pH after 5 days in PDB substratum of sour bacterium aspergillus niger XZ-127(is secreted by force in the strain of take in the present invention) describe as example, first to carry out antagonism test and secrete the possible restraining effect of sour bacterium to eliminate secreting sour bacterium and target white-rot fungi, concrete operations are as follows: (1L substratum comprises 20 grams of glucose will to be incubated at PDA substratum, 200 grams of potato leach liquors, 20 grams of agar) aspergillus niger on and Irpex lacteus are inoculated in respectively in the PDA cultivation in same culture dish, the about 3cm of distance, cultivate the form of observing afterwards two bacterium for 3-5 days, find to occur between two bacterium the situation of mutual inhibition, therefore can be used for degraded system.
Cultural method for the synthesis of the cultural method of secreting sour bacterium mycoderm of dyestuff degraded with white-rot fungi mycoderm, mycoderm after 5 days to the decolouring result of nine kinds of dyestuffs (100ml concentration is 100mg/l) as table 3.
Table 3 is secreted the decolouring to nine kinds of dyestuffs after 5 days of sour bacterium
| Testing index | Malachite green | Viola crystallina | Aniline blue | Orange G | Thymol blue | Methylene blue | Tetrabromo-mcresolsulfonphthalein | Tropeolin-D | Methyl red |
| Percent of decolourization | No bleaching | No bleaching | 8% | No bleaching | No bleaching | No bleaching | No bleaching | No bleaching | 11% |
(4) embodiments of the invention: compound system is processed experiment to the decolouring of combining of dyestuff.
The decolouring of compound system to dyestuff (100ml concentration is 100mg/l): adding successively Zero-valent Iron 1g, white-rot fungi mycoderm 8g(dry mycelium content in degraded system is 2%), secreting sour bacterium mycoderm 8g(dry mycelium content is 2%), within every 24 hours, measure a light absorption value, observe decolouring situation, the results are shown in Table 4.
The decolouring of table 4 compound system to nine kinds of dyestuffs
| Testing index | Malachite green | Viola crystallina | Aniline blue | Orange G | Thymol blue | Methylene blue | Tetrabromo-mcresolsulfonphthalein | Tropeolin-D | Methyl red |
| PH after 5 days | 2.5 | 5.5 | 3 | 3 | 3 | 3 | 3.5 | 3.5 | 3 |
| Decolour steady time (my god) | 2 | 4 | 1 | 2 | 3 | 2 | 2 | 1 | 1 |
| Percent of decolourization | 95% | 87% | 99% | 99% | 88% | 93% | 87% | 97% | 98% |
By table 1, table 2, table 3 and table 4 relatively, but find out the decolouring of compound system to nine kinds of dyestuffs, though longer than the Zero-valent Iron independent role time, percent of decolourization is higher, and percent of decolourization reaches more than 87%, decolours more thorough; Shorter than the independent decolorization of the white-rot fungi time, and percent of decolourization is higher.
Embodiment 2
From printing and dyeing mill, get 100 kilograms of waste water, the colourity of waste water is 50000 times, and the pH of pending dyeing waste water does not have special requirement, without the pH value that regulates waste water, the bacterial classification that the present invention adopts is good at processing waste water of basic printing and dyeing (most dyeing waste waters are alkalescence), applicable too at acid dyeing waste water.Secrete the cultural method of sour bacterium mycoderm and white-rot fungi mycoderm with embodiment 1, the white-rot fungi mycoderm of the present embodiment is selected Irpex lacteus to cultivate and is formed mycoderm, secreting sour bacterium mycoderm selects aspergillus niger XZ-127 cultivation to form mycoderm, wherein aspergillus niger XZ-127 wave and culture pH after 5 days in PDB substratum is 3.5, treatment process, with embodiment 1, adds separately iron powder, white-rot fungi mycoderm, secretes sour bacterium mycoderm, compound system.Wherein, iron powder is processed: in 100 kilograms of waste water, add 1 kilogram of iron powder; White-rot fungi mycoderm is processed: directly add cultured white-rot fungi mycoderm 8 kilograms (dry mycelium content is 2%) in advance to pour in waste water; Secreting sour bacterium mycoderm processes: directly add cultured 8 kilograms of the sour bacterium mycoderms (dry mycelium content is about 2%) of secreting in advance to pour in waste water; Composite degradation system: respectively by 1 kilogram of iron powder, 8 kilograms of white-rot fungi mycoderms, secrete sour bacterium mycoderm for 8 kilograms and add in waste water.Above-mentioned each processed all under field conditions (factors), and temperature is 25 ℃ of left and right, under condition of normal pressure, decolour, and every about 6 hours hand operated mixing 10 minutes, after decolouring is stable, measure percent of decolourization, colourity changes and to Ecotoxicology.
Ecotoxicology test adopts circle paper disk method, specific as follows: it is standby that the destainer after decolouring is stable carries out filtration sterilization with millipore filtration, take subtilis as test bacterium, with punch tool, filter paper is broken into the roundlet scraps of paper that diameter is 4mm, after high-temperature sterilization respectively with above-mentioned destainer soak take out in super task platform after approximately 3 minutes air-dry.With coating method, by 150 μ l concentration, be 10
6the bacteria suspension of individual thalline/ml is coated on the flat board of beef extract-peptone solid medium, is placed in afterwards 28 ℃ of constant incubators and cultivates 24h, measures inhibition zone size (reaction destainer toxicity size).
Comparative example and compound system of the present invention are as shown in table 5 to the result of dyeing waste water.In table 5, percent of decolourization is to calculate by the variation of light absorption value, and colourity is to calculate by the multiple that is diluted to distilled water color (appearance method).
The disposition of each method of table 5 to dyeing waste water
| Measurement index | Iron powder is processed | White-rot fungi mycoderm is processed | Secreting sour bacterium mycoderm processes | Compound system is processed |
| Percent of decolourization | 68% | 65% | 5% | 86% |
| Colourity after processing | 6800 times | 6200 times | 45000 times | 2100 times |
| Steady time (my god) | 3 | 7 | 6 | 4 |
| Antibacterial circle diameter (cm) | 1.6±0.3 | 1.1±0.2 | 2.1±0.3 | 0.6±0.1 |
Known by the result in table 5, compound system to the percent of decolourization of waste water from dyestuff apparently higher than other individual curing method, colourity obviously weakens compared with other method, and after processing, colourity is about 1/3 of iron powder individual curing and white-rot fungi mycoderm individual curing, and percent of decolourization reaches 86%; Treatment time also obviously shortens; The toxicity of microorganism is also reduced greatly, and antibacterial circle diameter is about 1/2 of iron powder individual curing and white-rot fungi mycoderm individual curing.
Embodiment 3
From printing and dyeing mill, get 100 kilograms of waste water, the colourity of waste water is 30000 times, secrete the cultural method of sour bacterium mycoderm and white-rot fungi mycoderm with embodiment 1, the white-rot fungi mycoderm of the present embodiment is selected Irpex lacteus to cultivate and is formed mycoderm, secreting sour bacterium mycoderm selects aspergillus niger XZ-127 cultivation to form mycoderm, wherein aspergillus niger XZ-127 wave and culture pH after 5 days in PDB substratum is 3.0, and treatment process, with embodiment 1, adds separately iron powder, white-rot fungi mycoderm, secretes sour bacterium mycoderm, compound system.Wherein, iron powder is processed: in 100 kilograms of waste water, add 1.5 kilograms of iron powders; White-rot fungi mycoderm is processed: directly add cultured white-rot fungi mycoderm 7 kilograms (dry mycelium content is 2.5%) in advance to pour in waste water; Secreting sour bacterium mycoderm processes: directly add cultured 9 kilograms of the sour bacterium mycoderms (dry mycelium content is about 1.5%) of secreting in advance to pour in waste water; Composite degradation system: respectively by 1.5 kilograms of iron powders, 7 kilograms of white-rot fungi mycoderms, secrete sour bacterium mycoderm for 9 kilograms and add in waste water.Above-mentioned each processed all under field conditions (factors), and temperature is 40 ℃ of left and right, under condition of normal pressure, decolour, and every about 7 hours hand operated mixing 10 minutes, after decolouring is stable, measure percent of decolourization, colourity changes and to Ecotoxicology.Ecotoxicology testing method is with embodiment 2.
Comparative example and compound system of the present invention are as shown in table 6 to the result of dyeing waste water.In table 6, percent of decolourization is to calculate by the variation of light absorption value, and colourity is to calculate by the multiple that is diluted to distilled water color (appearance method).
The disposition of each method of table 6 to dyeing waste water
| Measurement index | Iron powder is processed | White-rot fungi mycoderm is processed | Secreting sour bacterium mycoderm processes | Compound system is processed |
| Percent of decolourization | 69% | 63% | 5% | 88% |
| Colourity after processing | 4500 times | 4800 times | 27000 times | 1400 times |
| Steady time (my god) | 3 | 7 | 6 | 4 |
| Antibacterial circle diameter (cm) | 1.5±0.3 | 1.2±0.2 | 2.1±0.3 | 0.5±0.1 |
Known by the result in table 6, compound system to the percent of decolourization of waste water from dyestuff apparently higher than other individual curing method, colourity obviously weakens compared with other method, and after processing, colourity is about 1/3 of iron powder individual curing and white-rot fungi mycoderm individual curing, and percent of decolourization reaches 86%; Treatment time also obviously shortens; The toxicity of microorganism is also reduced greatly, and antibacterial circle diameter is about 1/2 of iron powder individual curing and white-rot fungi mycoderm individual curing.
Embodiment 4
From printing and dyeing mill, get 100 kilograms of waste water, the colourity of waste water is 10000 times, secrete the cultural method of sour bacterium mycoderm and white-rot fungi mycoderm with embodiment 1, the white-rot fungi mycoderm of the present embodiment is selected Irpex lacteus to cultivate and is formed mycoderm, secreting sour bacterium mycoderm selects aspergillus niger XZ-127 cultivation to form mycoderm, wherein aspergillus niger XZ-127 wave and culture pH after 5 days in PDB substratum is 3.5, and treatment process, with embodiment 1, adds separately iron powder, white-rot fungi mycoderm, secretes sour bacterium mycoderm, compound system.Wherein, iron powder is processed: in 100 kilograms of waste water, add 0.5 kilogram of iron powder; White-rot fungi mycoderm is processed: directly add cultured white-rot fungi mycoderm 9 kilograms (dry mycelium content is 1.5%) in advance to pour in waste water; Secreting sour bacterium mycoderm processes: directly add cultured 7 kilograms of the sour bacterium mycoderms (dry mycelium content is about 2.5%) of secreting in advance to pour in waste water; Composite degradation system: respectively by 0.5 kilogram of iron powder, 9 kilograms of white-rot fungi mycoderms, secrete sour bacterium mycoderm for 7 kilograms and add in waste water.Above-mentioned each processed all under field conditions (factors), and temperature is 20 ℃ of left and right, under condition of normal pressure, decolour, and every about 5 hours hand operated mixing 10 minutes, after decolouring is stable, measure percent of decolourization, colourity changes and to Ecotoxicology.Ecotoxicology testing method is with embodiment 2.
Comparative example and compound system of the present invention are as shown in table 7 to the result of dyeing waste water.In table 7, percent of decolourization is to calculate by the variation of light absorption value, and colourity is to calculate by the multiple that is diluted to distilled water color (appearance method).
The disposition of each method of table 7 to dyeing waste water
| Measurement index | Iron powder is processed | White-rot fungi mycoderm is processed | Secreting sour bacterium mycoderm processes | Compound system is processed |
| Percent of decolourization | 68% | 66% | 5% | 87% |
| Colourity after processing | 1800 times | 2000 times | 9000 times | 600 times |
| Steady time (my god) | 3 | 7 | 6 | 4 |
| Antibacterial circle diameter (cm) | 1.7±0.3 | 1.0±0.2 | 2.0±0.3 | 0.5±0.1 |
Known by the result in table 7, compound system to the percent of decolourization of waste water from dyestuff apparently higher than other individual curing method, colourity obviously weakens compared with other method, and after processing, colourity is about 1/3 of iron powder individual curing and white-rot fungi mycoderm individual curing, and percent of decolourization reaches 86%; Treatment time also obviously shortens; The toxicity of microorganism is also reduced greatly, and antibacterial circle diameter is about 1/2 of iron powder individual curing and white-rot fungi mycoderm individual curing.
In actual application, the temperature of reaction, Zero-valent Iron consumption, white-rot fungi mycoderm consumption, secrete the parameters such as sour bacterium mycoderm consumption and stirring cycle and can be undertaken preferably by economic and practical principle.
Claims (5)
1. a method for degrading synthetic dye through compound system, the steps include:
(1) dyeing waste water is joined in reactor, the colourity of pending dyeing waste water is equal to or less than 50000 times of colourities;
(2) respectively by Zero-valent Iron, white-rot fungi mycoderm with secrete sour bacterium mycoderm and successively add in dyeing waste water;
(3) add after above-mentioned processing material, the processing of decolouring, stirred once every 5 ~ 7 hours, until percent of decolourization and colourity keep stable.
2. the method for a kind of degrading synthetic dye through compound system according to claim 1, is characterized in that: the reaction that in step (3), decolouring is processed is carried out under field conditions (factors), and temperature is 20 ~ 40 ℃, and pressure is normal pressure.
3. the method for a kind of degrading synthetic dye through compound system according to claim 1 and 2, it is characterized in that: described white-rot fungi is Irpex lacteus, the cultural method of Irpex lacteus is that the Irpex lacteus of preservation is inoculated in potato liquid nutrient medium is housed, the leach liquor and the glucose that in substratum, comprise potato, under field conditions (factors), cultivate and form mycelium pellet after 4 ~ 6 days, under sterile state, be broken into bacteria suspension, drawing bacteria suspension puts to being equipped with in the culture dish of sterilizing potato liquid nutrient medium, under field conditions (factors), cultivate and form mycoderm after 4 ~ 6 days, inoculum as dyestuff degraded.
4. the method for a kind of degrading synthetic dye through compound system according to claim 1 and 2, is characterized in that: the described sour bacterium pH value of fermented liquid in substratum of secreting is less than 4.
5. the method for a kind of degrading synthetic dye through compound system according to claim 1 and 2, is characterized in that: described Zero-valent Iron is iron powder, and iron powder add-on is 1% of waste water weight; Described white-rot fungi mycoderm add-on is 8% of waste water weight; It is described that to secrete sour bacterium mycoderm add-on be 8% of waste water weight.
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| CN1843951A (en) * | 2006-04-19 | 2006-10-11 | 浙江大学 | Method for treating printing/dyeing waste water |
| CN102000541A (en) * | 2010-11-22 | 2011-04-06 | 广西大学 | Spongy bentonite-based composite adsorbing material and preparation method thereof |
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| CN102000541A (en) * | 2010-11-22 | 2011-04-06 | 广西大学 | Spongy bentonite-based composite adsorbing material and preparation method thereof |
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