CN102318896B - Cigarette harm reduction additive and preparation method thereof - Google Patents
Cigarette harm reduction additive and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a cigarette harm reduction additive, which comprises a plant extract composition, wherein the plant extract composition comprises licorice flavonoids, folium ginkgo extracts, epimedium flavonoids and astragaloside, the addition proportion of tobacco shreds into the additive is 0.001 to 0.05 weight percent, the additive comprises 15 to 84 weight percent of licorice flavonoids, 15 to 84 weight percent of folium ginkgo extracts, 0.3 to 20 weight percent of epimedium flavonoids and 0.5 to 25 precent wt of astragaloside and can further comprise plant extracts of total saponin from ophiopogon japonicus. The additive can externally inhibit the generation of harmful smoke substances such as polycyclic aromatic hydrocarbon, free radicals and the like, so the harm of smoke to people is effectively reduced. In addition, the invention also provides the preparation method of the cigarette harm reduction additive.
Description
Technical field
The present invention relates to cigarette additive and preparation field thereof, especially relate to a kind of composition of plant extracts cigarette harm reduction additive with reducing poison function.
Background technology
Composition in the cigarette smoke is very complicated, not only atmosphere is produced and pollutes, and also human health has been produced certain harm.At present research thinks that mainly containing harmful substances in the flue gas has polycyclic aromatic hydrocarbon and free radical etc.: polycyclic aromatic hydrocarbon belongs to very strong carcinogen; And free radical can be directly and the indirect aggression cell component, is the major reason that gives rise to diseases, and simultaneously at high temperature constantly polymerization of free radical forms different polycyclic aromatic hydrocarbons and is detrimental to health.
China's tobacco industry makes every effort to reduce these harmful substances to the infringement of health having carried out large quantity research aspect these harmful components of minimizing flue gas.The at present research of this respect relates generally to two aspects, and the one, traditional new technology of pass through, new technology, new equipment reaches the harm reduction purpose, as by various technology increase obbbin coil paper gas permeabilities; Increase expansion thread in the pipe tobacco, the stem consumption; Adopt the method such as nanometer technology to increase filter tip to the absorption of harmful substance etc.The 2nd, by in pipe tobacco and filter tip, increasing the additive derive from natural plants, by additive self component biological action or by its absorption to harmful matter in cigarette, the effect of degraded alleviates these harmful substances to body injury.
China just begins to carry out the research and development of Chinese medicine class natural plants additive cigarette since the middle of last century.The method that adopts in the industry at present, the one, traditional adds pipe tobacco with simple or extract, and the 2nd, with refining thing adding pipe tobacco or the filter tip of plurality of Chinese extract or extract.Produced thus some the cigarette that alleviates the damage by fume effect has been arranged.But at present for the additive research that derives from Chinese medicinal plant composition traditional handicraft extracts that are confined to also, shorter mention traditional Chinese medicinal components area research has no report especially for the use of traditional Chinese medicinal components compatibility additive more.The infringement of body health.
Description of drawings
Above-mentioned and/or additional aspect of the present invention and advantage are from obviously and easily understanding becoming the description of embodiment below in conjunction with accompanying drawing, wherein:
Fig. 1 is regression equation and the regression model chart that glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside four component compatibilities are removed DPPH;
Fig. 2 is the regression model chart that glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside four component compatibilities are removed polycyclic aromatic hydrocarbon.
The specific embodiment
The below describes embodiments of the invention in detail.Be exemplary below by the embodiment that is described with reference to the drawings, only be used for explaining the present invention, and can not be interpreted as limitation of the present invention.
Experimental example
One, experiment material
(1) reagent
Anthracene (Anthracene, 99%), U.S. ACROS ORGANICS product; DPPH, U.S. SIGMA company product; Hydroxy radical kit, Nanjing build up bio-engineering research institute product; Anti-ultra-oxygen anion free radical kit, Nanjing build up bio-engineering research institute product; Absolute ethyl alcohol, Chemical Reagent Co., Ltd., Sinopharm Group analyzes pure; Acetone, the Beijing Chemical Plant analyzes pure; Acetic acid (glacial acetic acid), the Beijing Chemical Plant analyzes pure.
(2) medicine
Ginkgo biloba p.e, 24% flavones, 6% lactone, Nanjing Lang Ze Pharmaceutical Technology Co., Ltd product; Glycyrrhiza total flavonoid, content 90%, Henan Bei Te bio tech ltd product; Dwarf lilyturf tuber total saponins, content 90%.The bright bio tech ltd product in sea, Shanghai; Barren wort total chromocor, content 70%, Nanjing Lang Ze medical sci-tech is limited thus need to provide a kind of and can make an addition in the cigarette, to the inhibited traditional Chinese medicinal components of harmful substances in flue gas.
Summary of the invention
One of purpose of the present invention is to provide a kind of cigarette harm reduction additive that contains composition of plant extracts.
According to one of purpose of the present invention, technical scheme of the present invention is as follows:
A kind of cigarette harm reduction additive, comprise composition of plant extracts, described composition of plant extracts comprises glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside, it is characterized in that: the ratio that described composition of plant extracts makes an addition to pipe tobacco is 0.001-0.05%wt, and the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 15-84%wt, ginkgo biloba p.e 15%-84%wt, barren wort total chromocor 0.3-20%wt, astragaloside 0.5-25%wt.
The proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 19-79%wt, ginkgo biloba p.e 19%-79%wt, barren wort total chromocor 0.6-3%wt, astragaloside 0.9-3%wt.
The proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 20%wt, ginkgo biloba p.e 78.5%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.
The proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 78.5%wt, ginkgo biloba p.e 20%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.
Further, described composition of plant extracts also comprises dwarf lilyturf tuber total saponins.
The proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 19.9%wt, ginkgo biloba p.e 78.0%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt, dwarf lilyturf tuber total saponins 0.6%wt.
The proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 78.0%wt, ginkgo biloba p.e 19.9%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt, dwarf lilyturf tuber total saponins 0.6%wt.
Compared with prior art, beneficial effect of the present invention is: cigarette harm reduction additive provided by the present invention has external scavenging action to flue gas harmful substance polycyclic aromatic hydrocarbon, free radical, can effectively reduce and extenuate to people company product; Astragaloside, content 50%, Nanjing Lang Ze Pharmaceutical Technology Co., Ltd.
(3) instrument
The F7000 sepectrophotofluorometer, HIT's product;
BT25S 100,000/electronic analytical balance, German Sartorius company product;
DU730 type protein nucleic acid analyzer, U.S. BECKMAN company product;
SUMSUNG DI-S24 type electric-heated thermostatic water bath, the gloomy reliable Instr Ltd.'s product of testing in Shanghai;
Chinese prescription Optimization Software (standalone version), Chinese department of traditional Chinese medicine institute Chinese medicine provides
Two, experimental technique
1, the determining of every kind of high low dosage of traditional Chinese medicinal components in the compatibility:
At first determine the proportion compatibility of ginkgo biloba p.e, glycyrrhiza total flavonoid, barren wort total chromocor and this four traditional Chinese medicine of astragaloside: adopt Radix Glycyrrhizae and ginkgo leaf to add up to and account for total amount 70%, astragaloside and barrenwort total account for 30% and are standard, under the constant prerequisite of total amount, with maxima solubility, the IC of the original medicine of each component in 70% ethanol
50Be foundation, the high low dosage of design drug component; According to removing DPPH and polycyclic aromatic hydrocarbon action intensity, adopt Uniform ity Design Method to obtain the four traditional Chinese medicine thing proportion compatibility of optimizing; Then add on this basis dwarf lilyturf tuber total saponins, according to the effect of removing DPPH, hydroxyl radical free radical, ultra-oxygen anion free radical and polycyclic aromatic hydrocarbon, integrated optimization goes out the best proportioning of this five kinds of Chinese medicine.
2, determining of each traditional Chinese medicinal components number of levels of uniform experiment design and each horizontal density degree:
What of Chinese medicine number of levels design, the accuracy that the number of times of direct relation test and testing site are selected.Should be greater than the several 2 times uniform Design principle of factor according to number of levels; At first, this experiment is for 4 kinds of traditional Chinese medicinal components, and institute thinks 4 factors; The overall design number of levels is 12, and wherein ginkgo biloba p.e and glycyrrhiza total flavonoid drug action are stronger, have designed 12 number of levels; Barren wort total chromocor and astragaloside, it is less to be subject to the investigation dosage range, adopts the pseudo level mode, designs 6 dosage levels, and circulation primary reaches 12 levels.So namely the number of levels with the interior other drug of prescription is consistent.Therefore adopt uniform designs table U12 (12
4) carry out dosage ratio relation research.Dosage design, even test calendar see Table 1-2.
Table 1 test dose design table
Table 2 U12 (124) Uniform Design calendar
3 detection methods:
The research of the external removing of traditional Chinese medicinal components additive compatibility DPPH free radical, hydroxy radical and ultra-oxygen anion free radical adopts AAS to detect, remove the effect of polycyclic aromatic hydrocarbon and adopt fluorescence spectrophotometry to detect, concrete experiment research is as follows:
(1) the traditional Chinese medicinal components compatibility is removed the assay method of DPPH free radical: it is 25mg/L (about 6.34 * 10 that DPPH is configured to mass concentration with absolute ethyl alcohol
-5Mol/L) for subsequent use.In test tube, add successively 2ml DPPH solution and 0.5ml 70% alcoholic solution (sample dissolution solvent), survey light absorption value (A) at 517nm wavelength place with the 1cm cuvette behind the mixing, be designated as A0; Add 2ml DPPH solution and 0.5ml traditional Chinese medicinal components test solution to be measured, leave standstill 30min at the dark place behind the mixing, measure and be designated as Ai; Add 2ml ethanol solution and 0.5ml traditional Chinese medicinal components test solution to be measured, measured value behind the mixing is designated as Aj, is calculated as follows free radical scavenging activity.Clearance rate (%)=[1-(Ai-Aj)/A0] * 100%.
(2) traditional Chinese medicinal components is removed the assay method of hydroxy radical and ultra-oxygen anion free radical: adopt Nanjing to build up hydroxy radical and ultra-oxygen anion free radical kit measurement, detect the acquisition inhibiting rate according to kit specification method;
(3) traditional Chinese medicinal components is removed the detection method of polycyclic aromatic hydrocarbon anthracene: anthracene is configured to the acetone soln use that mass concentration is 0.5ug/ml.Chinese medical extract is made into 70% ethanolic solution of variable concentrations.The sepectrophotofluorometer sweep parameter is set: excite and launch monochromator slit bandpass width to be 5nm, sweep speed 240nmmin
-1Excitation wavelength 265nm, emission wavelength 400nm.Pour test solution into quartzy liquid pool, place instrument sample room.Add the zeroing of 1ml 70% ethanol with 1ml acetone.Detect the variation of adding drug matching component front and back anthracene fluorescence intensity F, calculate the inhibiting rate of medicine.
Three, experimental result
1 four flavor traditional Chinese medicinal components optimum dose proportion researchs
1.1 four flavor traditional Chinese medicinal components proportionings are removed the uniform Design research of DPPH free radical and polycyclic aromatic hydrocarbon effect
According to the uniform experiment design of research method three, dispose respectively 12 initial mother liquors of drug concentration of this four flavors Chinese medicine of glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside, initial mother liquor compound concentration sees attached list 3; By each drug component mother liquor of getting respectively the respective concentration level shown in the table 2 according to 1: 1: 1: 1 mixes, and to mix mother liquor; Constant according to reaction system drug response final concentration DPPH to be that 0.05mg/ml, polycyclic aromatic hydrocarbon are constant be the standard of 0.012mg/ml, gets respectively these 12 and mix the mother liquor respective volume, adopts 70% ethanol to dilute, and obtains final 12 parts and mix liquid to be measured; Detect DPPH and the polycyclic aromatic hydrocarbon clearance rate of glycyrrhiza total flavonoid, ginkgo biloba p.e, 12 levels of these four flavor different proportionings of Chinese medicine of barren wort total chromocor and astragaloside, experimental result sees Table 4.
Table 3 four flavor traditional Chinese medicinal components initial formulation concentration and combination table
Table 4 four flavor traditional Chinese medicinal components uniform design combinations are to the clearance rate of DPPH and polycyclic aromatic hydrocarbon
Experimental group | The DPPH clearance rate | The polycyclic aromatic hydrocarbon clearance rate |
Close sequence number | (%) | (%) |
|
58.841±1.383 | 32.477±8.862 |
Experiment 2 | 71.208±1.590 | 42.871±3.047 |
|
45.217±0.547 | 38.736±1.491 |
Experiment 4 | 62.101±0.435 | 39.670±0.911 |
|
51.715±0.514 | 32.178±4.843 |
Experiment 6 | 64.324±0.711 | 40.928±2.833 |
|
46.184±0.209 | 38.145±3.596 |
Experiment 8 | 59.541±1.048 | 41.812±3.600 |
|
49.324±0.302 | 35.399±6.211 |
Experiment 10 | 62.609±0.261 | 40.683±0.218 |
|
44.251±1.653 | 37.353±4.928 |
Experiment 12 | 56.546±0.254 | 44.190±2.664 |
1.2 four flavor traditional Chinese medicinal components various dose proportionings are removed the LARS (minimum angular convolution is returned) of DPPH free radical and polycyclic aromatic hydrocarbon effect and are analyzed
Experimental result according to table 6, use " Chinese prescription Optimization Software ", adopt and improve regression equation and the regression model chart that the minimum angular convolution method of returning obtains respectively four component compatibilities removing DPPH and polycyclic aromatic hydrocarbon, wherein glycyrrhiza total flavonoid is X1, ginkgo biloba p.e is X2, barren wort total chromocor is X3, and astragaloside is X4, and average clearance rate is Y; The results are shown in regression equation and accompanying drawing 1-2:
1.2.1 four component compatibilities are removed regression equation and the regression model chart of DPPH
Y=(1348.41024529341)*X1+(1902.85081030458)*X2+(0.432341713582079)*X3+(-20.861132023621)*X4+(-11511.5692665415)*X1*X2+(6495.11575739643)*X1*X3+(0)*X2*X3+(0)*X1*X4+(18840.5788500999)*X2*X4+(0)*X3*X4+(1287.76729516979)*X1*X1+(0)*X2*X2+(0)*X3*X3+(0)*X4*X4。
1.2.2 four component compatibilities are removed regression equation and the regression model chart of polycyclic aromatic hydrocarbon
Y=(4344.30806655099)*X1+(3367.7600236284)*X2+(1932.36379982644)*X3+(-1025.30398350895)*X4+(0)*X1*X2+(0)*X1*X3+(85119.4913209487)*X2*X3+(0)*X1*X4+(0)*X2*X4+(1117335.08568284)*X3*X4+(28672.4751585725)*X1*X1+(40296.3665539673)*X2*X2+(0)*X3*X3+(0)*X4*X4
1.3 predict the outcome and confirmatory experiment: according to above regression equation, obtain respectively the Automatic Optimal Compatibility Results of this four flavors traditional Chinese medicinal components, in conjunction with actual production, again carry out artificial optimization's Compatibility Results, prediction is removed the DPPH effect and is combined as optimum to test 13, and experiment 14 combinations are taken second place; Remove the polycyclic aromatic hydrocarbon effect and be combined as optimum with experiment 13-1, experiment 14-1 combination is taken second place; According to predicting the outcome, be optimized compatibility combination and the actual confirmatory experiment that detects best of breed removing DPPH and polycyclic aromatic hydrocarbon; Experimental result is with in full accord by predicting the outcome of LARS analysis acquisition.Experimental result sees Table 5-6:
Prediction and the result of DPPH removed in table 5 four flavor traditional Chinese medicinal components uniform design optimum organizations
Remarks: above-mentioned dosage is original medicine configuration concentration
Prediction and the result of polycyclic aromatic hydrocarbon removed in table 6 four flavor traditional Chinese medicinal components uniform design optimum organizations
Remarks: above-mentioned dosage is original medicine configuration concentration
2 traditional Chinese medicine of the five flavours combination of components are removed multiple free radical and polycyclic aromatic hydrocarbon effect research
Optimum combination result and the actual production designing requirement of this four flavors Chinese medicine of ginkgo biloba p.e, glycyrrhiza total flavonoid, barren wort total chromocor and astragaloside that obtains according to early-stage Study add dwarf lilyturf tuber total saponins at above-mentioned Research foundation, and dosage is 0.024mg/ml; After adding dwarf lilyturf tuber total saponins, DPPH and polycyclic aromatic hydrocarbon clearance rate all increase than corresponding four traditional Chinese medicine thing combination; According to the comprehensive function of removing DPPH, hydroxyl radical free radical, ultra-oxygen anion free radical and polycyclic aromatic hydrocarbon, the best proportioning dosage that obtains this five kinds of Chinese medicine is glycyrrhiza total flavonoid 0.8mg/ml, ginkgo biloba p.e 3.144mg/ml, barren wort total chromocor 0.024mg/ml, astragaloside 0.036mg/ml and dwarf lilyturf tuber total saponins 0.024mg/ml.Experimental configuration ratio and the results are shown in Table 7-8;
Table 7 traditional Chinese medicine of the five flavours component uniform design optimum organization configuration dose ratio
Remarks: 1. above-mentioned dosage is original medicine configuration concentration; 2. * four traditional Chinese medicine thing combination+dwarf lilyturf tuber total saponins
Multiple free radical and polycyclic aromatic hydrocarbon effect are removed in the optimum organization of table 8 traditional Chinese medicine of the five flavours component uniform design
Remarks: * four traditional Chinese medicine thing combination+dwarf lilyturf tuber total saponins
Four, conclusion
Choose five traditional Chinese medicinal components such as glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor, astragaloside, dwarf lilyturf tuber total saponins, carry out the research that the Chinese medicinal formulae component is removed free radical and removed the best compatibility of polycyclic aromatic hydrocarbon effect and dose relationship, the result shows: the multiple ratio compatibility of glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside all has remarkable external scavenging action to free radical and polycyclic aromatic hydrocarbon, and effect is better than the simple component.Best of breed take removing DPPH as index is as glycyrrhiza total flavonoid 0.8mg/ml, ginkgo biloba p.e 3.144mg/ml, barren wort total chromocor 0.024mg/ml and astragaloside 0.036mg/ml, and proportion of composing is glycyrrhiza total flavonoid 20%wt, ginkgo biloba p.e 78.5%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt; Best of breed take the removing polycyclic aromatic hydrocarbon as index is as glycyrrhiza total flavonoid 3.144mg/ml, ginkgo biloba p.e 0.8mg/ml, barren wort total chromocor 0.024mg/ml and astragaloside 0.036mg/ml, and proportion of composing is glycyrrhiza total flavonoid 78.5%wt, ginkgo biloba p.e 20%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.On this basis, add dwarf lilyturf tuber total saponins 0.024mg/ml in above-mentioned four traditional Chinese medicine thing ratio, DPPH and polycyclic aromatic hydrocarbon clearance rate all increase than corresponding four traditional Chinese medicine thing combination, and proportion of composing is (1) glycyrrhiza total flavonoid 19.9%wt, ginkgo biloba p.e 78.0%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt, dwarf lilyturf tuber total saponins 0.6%wt;
(2) glycyrrhiza total flavonoid 78.0%wt, ginkgo biloba p.e 19.9%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.Take the comprehensive function of removing DPPH, polycyclic aromatic hydrocarbon, hydroxy radical and ultra-oxygen anion free radical as index, drug action is better than the simple component behind this traditional Chinese medicine of the five flavours component compatibility.
Embodiment one
Take by weighing the 20mg glycyrrhiza total flavonoid, 78.5mg ginkgo biloba p.e, 0.6mg barren wort total chromocor, 0.9mg astragaloside add 100ml 50% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 50% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment two
Take by weighing the 392.5mg glycyrrhiza total flavonoid, 100mg ginkgo biloba p.e, 3mg barren wort total chromocor, 4.5mg astragaloside add 100ml 70% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 70% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment three
Take by weighing the 520mg glycyrrhiza total flavonoid, 400mg ginkgo biloba p.e, 50mg barren wort total chromocor, 30mg astragaloside add 100ml 90% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 90% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment four
Take by weighing the 2.85g glycyrrhiza total flavonoid, 1.4g ginkgo biloba p.e, 500mg barren wort total chromocor, 250mg astragaloside add 200ml 60% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 60% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment five
Take by weighing the 30mg glycyrrhiza total flavonoid, 168mg ginkgo biloba p.e, 1mg barren wort total chromocor, 1mg astragaloside add 100ml 80% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 80% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment six
Take by weighing the 76mg glycyrrhiza total flavonoid, 316mg ginkgo biloba p.e, 4.4mg barren wort total chromocor, 3.6mg astragaloside add 100ml 95% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 95% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment seven
Take by weighing the 500mg glycyrrhiza total flavonoid, 1.3g ginkgo biloba p.e, 6mg barren wort total chromocor, 194mg astragaloside add 200ml 65% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 65% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment eight
Take by weighing the 1.6g glycyrrhiza total flavonoid, 2.2g ginkgo biloba p.e, 120mg barren wort total chromocor, 80mg astragaloside add 250ml 55% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 55% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment nine
Take by weighing the 622mg glycyrrhiza total flavonoid, 162mg ginkgo biloba p.e, 8mg barren wort total chromocor, 8mg astragaloside add 100ml 75% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 75% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment ten
Take by weighing the 1.26g glycyrrhiza total flavonoid, 225mg ginkgo biloba p.e, 4.5mg barren wort total chromocor, 10.5mg astragaloside add 100ml 70% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 70% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Take by weighing the 150mg glycyrrhiza total flavonoid, 125mg ginkgo biloba p.e, 100mg barren wort total chromocor, 125mg astragaloside add 100ml 65% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 65% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Embodiment 12
Take by weighing the 199mg glycyrrhiza total flavonoid, 780mg ginkgo biloba p.e, 6mg barren wort total chromocor, 9mg astragaloside, 6mg dwarf lilyturf tuber total saponins add 100ml 90% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 90% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Take by weighing the 1.56g glycyrrhiza total flavonoid, 398mg ginkgo biloba p.e, 12mg barren wort total chromocor, 18mg astragaloside, 12mg dwarf lilyturf tuber total saponins add 100ml 85% ethanol to sterile chamber, stir to make fully dissolving; Solution is crossed miillpore filter; Then supply 85% ethanol constant volume.Filtrate evenly is sprayed in the 10kg pipe tobacco, and get final product.
Claims (7)
1. cigarette harm reduction additive, comprise composition of plant extracts, described composition of plant extracts comprises glycyrrhiza total flavonoid, ginkgo biloba p.e, barren wort total chromocor and astragaloside, it is characterized in that: the ratio that described composition of plant extracts makes an addition to pipe tobacco is 0.001-0.05%wt, and the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 15-84%wt, ginkgo biloba p.e 15%-84%wt, barren wort total chromocor 0.3-20%wt, astragaloside 0.5-25%wt; Described Four Plants extractive composition has scavenging action to free radical, the polycyclic aromatic hydrocarbon compounds as the flue gas harmful substance.
2. described cigarette harm reduction additive according to claim 1, it is characterized in that: it is glycyrrhiza total flavonoid 19-79%wt, ginkgo biloba p.e 19%-79%wt, barren wort total chromocor 0.6-3%wt, astragaloside 0.9-3%wt that described composition of plant extracts gets proportion of composing.
3. cigarette harm reduction additive according to claim 1 and 2, it is characterized in that: the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 20%wt, ginkgo biloba p.e 78.5%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.
4. cigarette harm reduction additive according to claim 1 and 2, it is characterized in that: the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 78.5%wt, ginkgo biloba p.e 20%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt.
5. described cigarette harm reduction additive according to claim 1 and 2, it is characterized in that: described composition of plant extracts also comprises dwarf lilyturf tuber total saponins, after adding dwarf lilyturf tuber total saponins, five Extracts from Natural Plants strengthen the scavenging action of free radical and the polycyclic aromatic hydrocarbon effect than aforementioned Four Plants extract combination.
6. described cigarette harm reduction additive according to claim 5, it is characterized in that: the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 19.9%wt, ginkgo biloba p.e 78.0%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt, dwarf lilyturf tuber total saponins 0.6%wt.
7. described cigarette harm reduction additive according to claim 5, it is characterized in that: the proportion of composing of described composition of plant extracts is glycyrrhiza total flavonoid 78.0%wt, ginkgo biloba p.e 19.9%wt, barren wort total chromocor 0.6%wt, astragaloside 0.9%wt, dwarf lilyturf tuber total saponins 0.6%wt.
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