CN102311868B

CN102311868B - Method for extracting krill oil rich in phospholipids and neutral lipid without using solvent

Info

Publication number: CN102311868B
Application number: CN201110110688.5A
Authority: CN
Inventors: 斯科拉堡斯·卡特瓦斯·帝米垂; 拖若·谷尔雅·若奥; 琼·赖·马里奥
Original assignee: LUNSAR CO Ltd
Current assignee: LUNSAR CO Ltd
Priority date: 2011-04-29
Filing date: 2011-04-29
Publication date: 2017-05-24
Anticipated expiration: 2031-04-29
Also published as: CN102311868A

Abstract

The invention discloses a method for acquiring krill oil composition without using an organic solvent. The process comprises the following steps of: a) placing krills into a steamer, steaming for a period of time at certain temperature to denature protein contained in the krills, so as to obtain a primary solid product and a primary liquid product of the krills, wherein the condition that the primary solid product and the primary liquid product are formed into emulsion is avoided; b) under the condition that temperature is not less than 90 DEG C, taking out the primary solid product and the primary liquid product of the krills from the steamer container; c) separating the primary solid product from the primary liquid product; d) extracting the krill oil rich in neutral lipid from the primary liquid product; and e) squeezing the primary solid product to obtain squeezed liquid namely a secondary liquid product, and producing the krill oil rich in phospholipids from the liquid product. The method has the characteristic that no organic solvent is used in the whole separation and extraction steps. The invention also discloses composition of the krill oil produced by the method disclosed by the invention.

Description

A kind of solvent-free method for extracting the krill oil rich in phosphatide and neutral grease

Technical field

The present invention is on utilizing polyunsaturated fatty acid, DHA and 20 in the fractionating section of phosphatide Carbon 5 alkene acid extracts phosphatide oil, using astaxanthin and the neutral lipid from food production line, in synthesis krill meal fat content Human health is applied in the case of relatively low.The present invention also reveals one for obtaining the program of dry compound, wherein comprising with The phosphatide that DHA and eicosapentaenoic acid are connected is combined with krill oil, protein, and is applied to human health Astaxanthin.

Background technology

Krill abundant marine crustacean equivalent to a group of mean people in krill suborder, existence is being considered as all The original South Pole ocean of the forage reserve of Antarctic ecosystems.Krill, particularly survives in the South Pole and time South Pole region, It is made up of 11 kinds of krill species, as main Antarctic krill and brilliant krill.

In the last few years, huge profit was obtained as the potential source of protein and other dynamic biological products with krill Benefit.(Ellingsen, T.and Mohr, V.1979.Process Bioch.14：14；Suzuki, T.1981. fishes and shrimps protein Extractive technique, London, applied science publishing house) huge to krill expectation is derived from the huge life for being present in South Pole ocean Thing amount, estimates at 10 hundred million to 50 hundred million tons.The quantity of the catch of krill has been considered to that annual 5 hundred million to 10 hundred million tons can be reached, and this is suitable In the annual fishing output in the whole world (Budzinski, E., Bykowski, P.and Dutkiewicz, D.1986.Posibilidades de elaboración y comercialización de productos Preparados a partir de krill del Antartico, FAO Doc.T, c.Pesca (268)：47p).

The content of krill lipid is referred to by several publications and is constituted.(Grantham, G.J.1977.The Southern Ocean.The utilization of Krill.Southern Ocean Fisheries Survey Programme, Rome.FAO GLO/SO/77/3：63p；Budzinski et al.1986.Loc.cit.；Ellingsen and Mohr.1979.Loc.cit.).Lipid content accounts for the 10-26% of krill net weight, and this depends on season, the sexal maturity of krill Degree, also its body size.Usual krill fat content period high is during annual March to June.The phosphorus of female krill Fat content is the twice of male krill.Phospholipid concentration rises, the dramatic decrease after ovulation with the rise at krill age.Krill fat Class distribution research shows the region of Lipid rich in digest tube, between muscle bundle and ectoskeleton.(Saether, O., Ellingsen, T.and Mohr, V.1985.Comp.Biochem.Physiol.81B：609).

The fractionating section of main krill phosphatide includes triglyceride, also phosphatide, sterol and Ester. (Grantham.1977.Loc.cit.；Budzinski et al.1986.Loc.cit) phosphatide average content it is left 69% The right side, triglyceride is 26% or so.In the fractionating section of krill phosphatide, polyunsaturated fatty acid is very abundant, especially there is 20:5 With 22:6, equivalent to close to the 50% of all content of phospholipid.

Krill phosphatide is described by several publications to constitute.Most relevant of which statement has following several：

1st, Gordeev et al statements krill contains the extracted phosphatide of its weight in wet base 5%, more than the one of phospholipid form More than half, wherein lecithin (33-36% of all lipid contents), phosphatidyl-ethanolamine (1517%), lysophosphatidylcholine (3- 4%), other (2-3%) --- while triacylglycerol (32-35%) occupies the majority in without phosphorus composition.In first two phosphatide Prevailing fatty acid residue is multikey unsaturated acids residue (exceeding the 40% of acyl residue), eicosapentaenoic acid residue Quantity (C20:5w3) it is about 13% and 28%.(Gordeev, K.Y.et al.1990.Fatty acid composition of the main phospholipids of the Antarctic krill Euphausia superb.Chemistry of Natural Compounds.26：143-147)

2nd, the lipid composition of Fricke et al. statements krill is lecithin (33-36%), phosphatidyl-ethanolamine (5-6%), triacylglycerol (33-40%), free fatty (8-16%) and sterol (1.4-1.7%).Wax ester and sterol ester are only Have and occur in vestige.Can be defined more than 50 kinds of aliphatic acid, main has 14:0,16:0,16:1 (n-7), 18:1 (n-9), 18:1 (n-7), 20:5 (n-3) and 22:6(n-3).The content of phytanic acid accounts for the 3% of all content of fatty acid.Short chain, middle chain and Hydroxy fatty acid (C≤10) can not be detected.The fractionating section of sterol includes cholesterol, and desmosterol and 22- dehydrogenations courage are solid Alcohol.(Fricke, H.et al.2006.Lipid, sterol and fatty acid composition of Antarctic Krill (Euphausia superba Dana) .Lipids, 19：821-827)

3rd, Falk-Petersen et al statements, the lipid of north and south poles krill class shows its lipid content and season Relevant, fat content is high, and is neutral lipid, either wax ester or triacylglycerol, and major storage is used to breed.The arctic is peaceful Foreign krill and South Pole crystalline substance krill are with those arctic Thysanoessa raschii and South Pole Thysanoessa macrura and south Very big krill is compared, and comprising more wax esters and higher concentration 18:4 (n-3) and 20:5 (n-3), contain in its neutral lipid The 18 of lower ratio:1 (n-9)/(n-7) aliphatic acid.A large amount of leaves in the winter time in the lipid of T.raschii and South Pole crystalline substance krill are green Alcohol illustrates its intake for marine alga in sedimentation material and sea ice.Thysanoessa raschii, T.macrura, and the South Pole are big Krill has the aliphatic acid 18 of height ratio:1 (n-9)/(n-7), shows that it is carnivore (Falk-Petersen, S.et Al.2000.Lipids, trophic relationships, and biodiversity in Arctic and Antarctic krill.Can.J.Fish.Aquat.Sci.57：178-191).

4th, Clarke states the lipid content and composition of Antarctic krill.In summer, magnetic ovary maturity, in lipid total amount Rise, this has been also demonstrated that the krill lipid content come in interim male krill and krill cub when winter rises.Lipid storage master If triacylglycerol and less than 1% wax ester.Aliphatic acid is moderately unsaturated, although the lipid of ovary is fewer, triacylglycerol bag Containing the phytanic acid more than 4%.(Clarke A.1984.Lipid content and composition of Antarctic Krill, Euphausia superba Dana.J.Crust.Biol.4：285-294)

5th, the euphausiids collected on elephant island between Phleger et al. are expressed in 1997 to 1998, south Very big krill, the lipid composition of E.tricantha, E.frigida and Thysanoessa macrura.TL is big in the South Pole Krill highest (16mgg-1 weight in wet bases) in petticoats, the scope in other krill species is 12mg to 15mg.Polar lipid (total fat The 56-81% of matter) and triacylglycerol (12-38%) with only E.tricantha occur a wax ester be major lipids.Cholesterol is Main sterol (80-100% of total sterol amount), desmosterol is quantitatively deputy.1997 T.macrura and the South Pole Large krill includes a greater variety of sterol types, and comprising 24-nordehydrocholesterol (0.1-1.7%), dehydrogenation courage is consolidated Alcohol (1.1-1.5%), brassicasterol (0.5-1.7%), 24- methylenecholesterols (0.1-0.4%), and two kinds of smooth alcohol of department (0.1-0.2%).Monounsaturated fatty acids mainly includes 18:1 (n-9) c (7-21%), 18:1 (n-7) c (3-13%) and 16:1 (n-7) c (2-7%).Main saturated fatty acid is 16 in krill:0 (18-29%), 14:0 (2-15%) and 18:0 (1-13%). Highest eicosapentaenoic acid [EPA, 20:5 (n-3)] and DHA [DHA, 22:6 (n-3)] in Antarctic krill (EPA, 15-21%；DHA, 9-14%) middle appearance is quantitatively fewer than other kinds of krill.With the Antarctic krill of food grass (3.7-9.4%) is compared, in E.tricantha, E.frigida and T.macrura (0.4-0.7% of TFA) 18:4 (n-3) contents are relatively low, and this is also more consistent with food meat and omnivorous factor with it.Polyunsaturated fatty acid (PUFA) 18:5 (n-3), long-chain (VLC-PUFA), C (26) and C (28) PUFA, in not appearing in 1997 sample, but most of It is found in the low layer secondary pollutants of Euphausiacea in 1998.(Phleger, C.F.2002.Interannual and between Species comparison of the lipids, fatty acids and sterols of Antarctic krill from the US AMLR Elephant Island survey area.Comp Biochem Physiol B Biochem Mol Biol.131：733-747)

6th, Kolakowska states 7 kinds of fresh and krill samples after 215 degree (absolute temperature) difference storage period Lipid composition.Fresh krill lipid composition is different from the sample of frost, and this depends on memory period, harvest season, and hair Educate degree.Phosphatide demonstrate its to change it is very sensitive, this is opposite with most resistive triglyceride；Equally destroy glycerine Diester and cholesterol ester, frost program itself only minimal effect lipid composition；But, after 30 days storage periods, free-fat The content of acid almost doubles.After being stored 6 months under 251 ° of K, 70% phosphatide is decomposed, and the quantity of free fatty increases It is 6 to 20 times.Monoglyceride, is not present in fresh krill, occurs after several months frost storage.Childhood, krill was to fat The change of decomposition is more sensitive.Phosphatide of the female krill with mature egg comprising temperature；Only triglyceride is hydrolyzed (Kolakowska A.1986.Lipid composition of fresh and frozen-stored krill.Z Lebensm Unters Forsch.182：475-478).

7th, Bottino describes two kinds of krill purpose lipids compositions.In Antarctic krill, complex lipid is glycerine three Major lipids species before acid esters.In the crystalline substance krill of the South Pole, complex lipid is equally major lipids species, but second is main Inscape is ceride.Two kinds of complex lipids of krill are main by lecithin, also less amount of phosphatidyl-ethanolamine and haemolysis Phosphatidyl choline is constituted.The phosphatide of South Pole crystalline substance krill wants saturation than Antarctic krill.Wax in the crystalline substance krill of the South Pole is mainly oleic acid Esters (84%), palmitic acid (10%) and n- tetradecanols (69%) and n- hexadecanols (28%).(Bottino, N.R.1975.Lipid composition of two species of Antarctic krill：Euphausia superba and E.crystallorophias.Comp Biochem Physiol B.50：479-484)

8th, the WO02/ that EP1997498 and Neptune Technologies ＆ Bioressources Inc. possess 102394 is related to krill oil, consistent with acetone, refines lipid.By filtering, protein and krill material are removed from lipid. The water of acetone and residual is removed by evaporation.Content of phospholipid is 38-50%, and EPA and DHA content are 22-35%, wherein these The major part of omega-3 is attached to phosphatide.

9th, the WO2008/117062 that US2008/0274203 and Aker Biomarine ASA possess.These application explanations With a large amount of phosphatide, the new krill oil composition that astaxanthin ester and/or omega-3 are characterized.Krill oil is with comprising about 30-60% W/w phosphatide and about 20-35%omega-3 saturated fatty acids are characterized, and the wherein major part of these omega-3 is attached to phosphorus Fat.

The polyunsaturated fatty acid of high content in the marine low temperature of the South Pole for keeping plasma membrane in phosphatide fractionating section Flowing is necessary effect.Undersaturated phosphatide highly high is that krill keeps necessity of animal reflection and motion flexible at low temperature Property.

The increase of TL is along with the reduction of phosphatide and the increase of triglycerides in krill.Polyunsaturated fatty acid contains Amount is reduced and shown along with the change of increase (Saether et al.1985.Loc.cit) krill lipid of total lipid content, 0 DEG C when, polyunsaturated fatty acid (20:5,22:6) compare, the krill storage (16 of content of fatty acid:0) will not reduce.These numbers According to showing, it is stored in 0 DEG C of krill, the phenomenon of a large amount of polyunsaturated fatty acid oxidations will not be sent out after shellfish death It is raw.

There are several file descriptions to extract the commercial run of krill oil：

These files include：

1st, Budzinski et al. (1986.Loc.cit.) and Saether et al. (1985.Loc.cit.), description With the process of Solvent Extract methods krill lipid.

2nd, file CA2346979, the ES2306527 or UA75029 that holy Brock University delivers.File describes to use third Ketone from ocean and aquatile, the method that lipid fractionating section is extracted such as in krill.

3rd, the WO2006/106325 that Pro-Bio Group AS are delivered.This documents describes the acquisition phosphatide from krill Process.The process is contained and allows organic solvent exposure krill meal, to produce the liquid comprising lipid.Selectively, can be at this Other organic solvents are added to extract neutral lipid in individual liquid.Remaining fractionating section is the abundant part of phosphatide.

4th, the EP1997498 and WO02/ that Neptune Technologies ＆ Bioressources Inc. are delivered 102394.These files describe to use ketone solvent, preferably acetone, and lipid, or material derived from krill are extracted from krill.

5th, the GB407729 that Johan Olsen Nygaard are delivered.This patent describes the container by heating charge of oil, Grease is extracted from marine organisms, particularly whale and other marine mammals.This file be from our invention it is different, Because we are not to extract lipid using the method for heating container.Our invention extracts phosphatide without heating oil.

6th, WO2009/027692 shows the process of the multi-step for extracting krill and Related product.This process includes Krill is heated approximately at 75 DEG C first, hereafter screening is obtained without phosphatide krill.It is this to be heated to for the second time without phosphatide krill 85 DEG C, pressing is sieved through again.Remaining krill liquid (cream colored) is solidified in the step of heating for first, to remove protein And phosphatide.

This 5 files are that description utilizes organic solvent, particularly acetone to extract krill lipid fractionating section.These sides Method is different from program described herein, because the present invention is not that refining lipid is extracted from krill using organic solvent.

1st, JP58008037, Nippon Suisan Kaisha Ltd.Presents is indicated and extracted from antarctic krill oil The method of eicosapentaenoic acid or derivatives thereof.By traditional approach, krill oil is saponified into free fatty or ester, such as soap Change or alcoholysis, be the product of combination, collecting a main fractions by not engler distillation includes 40% weight or similar material.It is main It is that relatively low unrighted acid is eliminated with urea to want cut.This file and difference of the present invention are consummate lipid During still-process utilization.

2、JP2004024060(Nippon Suisan Kaisha Ltd).Obtained this document describes from krill Take the process of the lipid containing abundant astaxanthin.The process of this profile formulation is that its is short with process difference of the invention Phase target is not the production of astaxanthin.

3、US2008/0274203(Aker Biomarine ASA).This file is described to obtain krill oil, at one Two benches use supercritical fluid extraction program from krill meal.Stage one is by extracting pure postcritical CO₂Or CO₂, also Cosolvent close to 5% removes neutral lipid.Stage two is by using supercritical CO₂It is combined with the ethanol close to 20%, To extract actual krill oil.This file with present invention is different in that this program not use supercritical fluid extraction.

4th, WO2007/080514 (Krill A/S and Alfa Laval Copenhagen A/S) is described from krill The method of middle extraction lipid, wherein the krill of new capture is ground to form into slurry, was slowly heated to less than 60 DEG C, most within 45 minutes Fortunately less than 30 DEG C, hereafter, liquid splits into water phase and oil phase krill, and its a krill oil extract is without using organic Solvent and obtain.The program that this part of file shows is different with the present invention, and a present invention declares mill before process is not heated and arrests krill.This Heating-up temperature is more than 90 degrees Celsius during file and the present invention are also different from the program of the present patent application；Another main distinction is with regard to this Stated in file processes, krill is used to produce emulsification before producing ground slurry heating, hinders centrifugation phosphatide.At this The slurry of invention is not that the direct or indirect further WO2007/080514 for producing uses krill oil to separate ultrasound but this hair It is bright not use any kind of ultrasonic technology.It also proposed a simple extraction, and the present invention is with double extraction principles Based on.

5th, WO2007080515 (Aker Biomarine ASA) describe by less than 60 degree Celsius of temperature with One kind that the method that chemically and physically method decomposes lipid cell membrane to promote low temperature to extract processes krill and obtains krill fat adds Work method.This processing method is carried out in inert gas environment, in case oxidation or fat are rotten with protein.Middle Reason tank is maintained at floor level to reduce the residence time；The oil plant of recovery will freeze to keep stabilization immediately.Due to this instant Program be instead of using the heating more than 90 degrees Celsius, and the krill of capture will not be pulverized to extract lipid, therefore herein The processing method of part description is different from existing invention, in addition, the procedure of existing invention does not use any kind of gas Or Refrigeration Technique.

6th, WO2008/060163 (Pronova Biopharma Norge AS) is described comprising ethanol, methyl alcohol, propyl alcohol Or the program of krill fat is obtained under the supercritical pressure of isopropanol using carbon dioxide.Due to existing program using carry or Without the CO 2 supercritical temperature lipid extraction method of lytic agent, therefore the program that presents is described is with existing invention not Together.

7th, WO2009/027692 (Aker Biomarine ASA) describes a kind of method for producing krill meal.This side Method uses two step cooking programs.The first step, protein and phosphatide are extracted from krill and coagulation is precipitated.Second step, cooks Krill without phosphatide.Afterwards, using the residue fat and astaxanthin of mechanically decoupled method removal krill.In this method In, krill is heated to 60-75 degrees Celsius, by lipid and proteolytic/be distributed in water, to be made krill milk in water.This Kind krill milk is heated to 95-100 degrees Celsius with sediments such as the krill protein and lipid gone in water removal.Due to existing invention Middle krill oil does not have the step of avoiding heating for multiple times with coprecipitation mode separation, and new procedures from shellfish, because The program that this existing invention is used is different from the program that presents is illustrated.

On the ship of some factories, from krill meal machining production line traditional at present, only a small amount of krill oil is produced It is raw.This krill generally oil is rich in neutral fat, and wherein content of phospholipid is very low or even can't detect (＜ 0.5%).Generally, exist During by PROCESS FOR TREATMENT krill of uniting, fresh krill rotates this indirectly heat cooker and heats with conveying worm, afterwards By twin-screw press and drying.The liquid that twin-screw press is squeezed out, insoluble solid is removed by a bottle.Filtering Liquid afterwards is separated by the krill oil and astaxanthin that centrifuge will be enriched in neutral fats.Phosphatide and filtering in this traditional handicraft To solid matter in protein combine.Then, phosphatide is generally all present in krill meal.When krill is rich fatty Phase, the krill of fat content is typically 16-18% in meals.In the same period, using conventionally produced rich in neutral fats Fat krill oil ground krill powder yield is very low, generally accounts for the 0.3-1.0% from raw material krill.This neutral fats krill oil contains rich Rich astaxanthin fishes for situation between 700-1.500 mgs/kg depending on season and fishing ground.

If additionally, using a unconventional krill meal process, can also obtain above-mentioned similar result.Generally In the case of, non-traditional krill meal factory uses synthermal system, two-phase water decanter or three-phase water decanter and dryer.These utensils For well-done krill to be dehydrated and degreasing.Liquid after filtering is inserted in sleeping spiral shell centrifugation to obtain the krill rich in neutral fat Oil, its content of fatty acid is low or even can't detect (＜ 0.5%).In this case, phosphatide is also and the solid matter that is filled into In protein combine.As described above, the krill meal that phosphatide is found.

In this case, the krill oil yield rich in neutral lipid of non-traditional krill meal production line is much lower. The fatty amount of krill period high, yield is about the 0.1~0.4% of undressed krill amount.It is traditional synthermal in this course Cooker system is used for having inherent stirring (scraper).Therefore, treated krill is stirred and pulverizes.The mistake for stirring/milling The protein of krill and moisture are generated emulsified water by journey.Additionally, krill catalysis phosphatide emulsification, because lipid is equivalent to emulsion process Catalyst.For this reason, using non-traditional krill oil production technology, fat content higher enters in decanter bottle Solid portion.In krill meat fertile period, the fat content 1.0~2.5% of the flat liquid part of decanter, resulting Huang Color mucus, fat emulsification scope 0.7~1.6%, krill meal has a fat content range 20~26%, by this non-traditional Technique krill oil recovery rate is very low.

Fatty group of the phosphatide oil rich in neutral fat obtained using traditional and unconventional krill manufacturing technique method Divide and fatty acid component is closely similar：The triglyceride of content about 86-89%, phosphatide does not detect (content ＜ 0.5%), content The DHA and EPA of about 4-6%.By the method with industry application scale related to krill meal drink, make a kind of with DHA And EPA, the krill oil rich in phosphatide and low fat content, have attempted to make an effort.Several different operation temperatures, different torsions Square, high pressure, using two operating procedures, before using second decanter bottle, first decanter bottle is cleaned with mucus, such as The test that electric endochylema is separate has been carried out.But result does not all succeed.

It is conceived to the problem for separating the krill oil rich in phosphatide and the krill oil rich in DHA and EPA, some extracting methods are Through appearance and patented protection.

It is related to a kind of use high temperature to be fractionated and separate the patent application for obtaining krill oil method, its Patent No. CL10211995(Tepual SA).The production process of this krill oil is required to adjust the type of aliphatic acid Control in process of production makes the lipid composition of krill oil temperature, in (95 DEG C) operations of high temperature and relatively low multiple unsaturation Yield under, and the contrast that when operation temperature is reduced (75 DEG C) carry out phospholipid moiety quantum of output, and this krill oil produces The content of the key component correspondence triglyceride of technique is 35~96%.The content of phosphatide is 4~28%.Polyunsaturated fat Acid content is 4~46%.On the whole, this process is used to obtain krill oil, including culinary art krill, extrudes the krill cooked, The moisture that will be extruded isolates insoluble solid matter by two phase place separator, and liquid substance is isolated by centrifuge Oil.In the process, a kind of krill oil can only be obtained.

The content of the invention

Present invention is disclosed a Ge, (land) can obtain simultaneously (sea) or factory on ship：1) it is rich in The krill oil of phosphatide, and the 2) krill oil rich in neutral fat, wherein blue or green containing DHA and EPA polyunsaturated fatty acids and shrimp Element.The content of DHA and the EPA containing phosphatide is particularly high in antarctic krill oil.Due to the double property of the water fat of phosphatide, (its structure one Lipophilic group is contained at end, and one section contains hydrophilic group), it is extraordinary emulsifying agent.Rolling for krill is included with whipping process The emulsification of soluble protein, water, neutral fats and phosphatide.Therefore, once foring emulsion process, only by organic solvent or CO₂Critical extraction just can obtain phosphatide from krill mixture.Example refer to WO02/102394 and US2008/ 0274203.Advantage of the invention is that of avoiding and uses extractor, and the stirring on stream hereinafter mentioned/roll krill.

In the present invention, the new mistake that phosphatide is extracted from fresh krill or other similar shell adding class animals is disclosed Journey.Do not include rolling and stirring krill during being somebody's turn to do.With it, will not also produce the emulsion of phosphatide.First, cook Complete krill, separates its liquid substance for including, and solid-state and liquid part are generated for the first time.In the liquid of first time The oil that body portion includes is rich in neutral fat.The first time solid portion that culinary art is obtained can be separated further, be obtained Second solid-state and liquid part.The oil obtained from second liquid part is equally rich in phosphatide.Secondary solid-state portion Lease making crosses treatment and can obtain low fat krill meal.

Extraction process of the invention is more efficient, and preferably just fished for up in krill is carried out immediately.The invention is best It is to be carried out in overtime work, thus can preferably controls the process extracted, and unnecessary logistics fortune can be significantly decreased The loss caused in defeated process and transport.It is of the invention another advantage is that obtaining product under the similarity condition fished for and process Can just have the feature of finished product.

In process of the present invention, it is also possible to for other similar shellfish, whether cultivate or capture, such as shell-fish Animal, but be not limited to Pandalus borealis, Cervimunida johni, Heterocarpus reedi, Pleuroncodes monodom, Penaeus vannamei, Penaeus monodon, Penaeus stylirostris, Penaeus chinensis, Penaeus.orientalis, Penaeus japonicus, Penaeus indicus, Penaeus merguiensis, Penaeus esculentus, Penaeus setiferus, Macrobrachium spp and Other.Additionally, process as herein described can be used to obtain various fish and/or algae species oil, extract and useful Composition.In such scheme implementation process, krill can be replaced by any fish or algae.

According to the one side of invention, solvent-free production krill oil process includes：

A) cook krill, it is preferably fresh, the time of culinary art and temperature be enough to by krill be transformed into one it is separable just Level solid product and primary liquid product, while to be avoided that krill emulsification in cooking process；

B) primary stage solids product and primary liquid product are separated；And

C) can not during extracting krill oil, the separation and extraction from primary stage solids thing and primary liquid thing respectively Use organic solvent.

In a kind of method that the invention of related fields produces krill oil there is provided solvent-free process, including：

A) krill is cooked in cooker, preferably fresh krill, the time of culinary art can guarantee that the temperature of krill reaches 90 DEG C, And krill is become that the form of primary stage solids product and primary liquid product can be separated into, and generally ensure to avoid krill Emulsification.

B) in the case of ensureing that temperature is not less than 90 DEG C, will be well-done, it has been separated into the phosphorus of solid and liquid form Shrimp is taken out from cooker；

C) primary stage solids product and primary liquid product are separated；And

D) krill oil is extracted from primary stage solids product and primary liquid product respectively, must not be used in said process organic Solvent.

Krill oil is extracted from primary liquid product, primary liquid product will be separated into krill oil and water.

The primary stage solids product obtained by culinary art, is preferably further processed as described above：

A () is squeezed primary stage solids product, to obtain secondary solid product and secondary liquid product；And

B) secondary liquid product is separated into krill oil and water

Preferably as a result, krill oil in primary liquid product rich in neutral grease the krill in secondary liquid product Oil is rich in phosphatide.

The feasible solution that krill oil and water are isolated from secondary liquid product is that oil is separated from the water out with separator Come, or it is by drying that water is evaporated from oil.

Used as a part for existing invention, this invention utilizes equipment means, it is to avoid or Phospholipids generation is reduced as far as possible Emulsification, so that Phospholipids is separated from krill.The equipment be from traditional equipment and selection operation, with avoid stirring, Chopping, and/or grinding, whole all of process step (as cooked, pump etc.), to reduce emulsification and the protein to oil, water With krill phosphatide, this last component is used as emulsifying agent agent.These equipment include screw pump, use screw low speed, Belt Conveying Machine, conveying worm removes serious mistake, chopper or the continuous cooker of similar conveyer belt.

Other equipment may be used, and can avoid the step of excitement and/or grinding, be included in all for the treatment of：

1. the shellfish peeling machine such as krill

2. the shellfish such as krill brush peeling machine

3. the shellfish dise chipper such as krill

4. the shellfish such as krill Slow cure agent machine

5. the shellfish such as krill and low speed dewaterer

6. the shellfish bearder such as krill.

The krill in a kind of method that specific krill oil is extracted from krill of the present invention, the preferably Antarctic Continent south, can Krill is processed in advance immediately on way (at sea) with trawler or Mother Vessel.Further enter on land again Row treatment.Final processing method disclosed by the invention is comprised the following steps：A) marine product of capture is not being stirred and is not being cut Cooked in the case of broken；B) cooked marine product is separated, for example, using two-phase and/or three-phase The separator of water decanter or any other type, separates to marine product, to obtain a part degreasing and go consolidating for water State product, i.e. primary stage solids product, drain liquid, primary liquid product.C) by the degreasing of pressure extrusion part and straining for dehydration The liquid of hydrophone, and/or double helix pressure and/or other pressure apparatus are used, acquisition can be further processed into the dry of low fat Marine organisms food the second solid portion and the second liquid part；D) by centrifugation hydraulic pressure, phosphatide concentration is obtained Marine organisms grease；E) by separating the water decanter liquid that b) step is obtained, the concentration marine organisms grease of neutral lipid is obtained With thick juice.Stirring (for example, cook, inflate etc.) in the device that this stage uses avoids whole step, reaches Reduce the purpose of the oil emulsion containing protein, water and marine organisms phosphatide so that last element serves emulsifying agent Effect.

The present invention also includes that phosphatide, neutral lipid, the purposes of omega-3 and protein compression dry powder and this product are It is the health-oriented products that the mankind use, it can effectively advance health, strengthen growth, promotes immune system, be good health Product.Product disclosed in this invention is non-chemical methods and produces, for example, in the production process for separating krill oil not Use organic solvent, and/or CO₂Intermediary liquid.

Phosphatide concentration shrimp sauce can be broadly defined herein as including following material：

A () content is about the phosphatide of the 30% to 70% of weight；

B () content is about the DHL and EPA of the 10% to 70% of weight；

C () content is about 30% to 70% neutral lipid of weight；

D () content is about the astaxanthin of 200 to 1,500mg/kg；

E () content is about the phosphatidylserine of the 1.5% of weight or more lipid total amount；

F () content is about the lysolecithin equal to or less than the 0.6% of lipid total amount；

G () content is about the FFA equal to or less than the 3.4% of lipid total amount.

In phosphatide (PL) the concentration krill oil that the present invention is obtained, the content of phosphatidylserine (PS) is about 1.5% or more It is many, this solvent used to extract phosphatide (PL) concentration krill oil with those, and/or CO₂The prior art phase such as intermediary liquid Than being unique.In those prior arts, phosphatidylserine (PS) otherwise content be zero, or be less than 0.5%.

Phosphatide (PL) concentration krill oil of the invention is beneficial to health.The content of its phosphatidylserine is about More preferably, 35%-70%w/w is best for 30%-70%w/w, 35%-60%w/w.The content of DHA and EPA adds up to 10%-70% More preferably, 45%-65%w/w is best for w/w, 40%-65%w/w.The content of neutral lipid is about 30%-70%w/w, 40%- More preferably, 45%-65%w/w is best for 65%w/w.The content of astaxanthin is about 200-1, and 500mg/kg, 300-1,200mg/kg are more Good, 400-1,000mg/kg is best.

Neutral lipid can be broadly defined herein as including following material：

A () content is about the neutral lipid of weight 50%-100%；

B () content is about weight 2%-45%DHA and EPA；

(c) content be about weight be or less than weight 10% phosphatidylserine；

D () content is about the astaxanthin of 200-1,500mg/kg；

(e) free fatty acid content be about or less than weight 3.4%.

Neutral lipid astaxanthin of the invention is equally also advantageous for health.The content of its neutral lipid is about 50%- More preferably, 70%-100%w/w is best for 10%w/w, 60%-100%w/w.The content of DHA and EPA adds up to 2%-45%w/w, More preferably, pole 5%-35%w/w is best for 2%-40%w/w.The content of phosphatide is less than 10%w/w, less than 5%w/w more preferably, 2%w/ w.It is best.The content of astaxanthin is about 200-1, and more preferably, 400-1,000mg/kg is best for 500mg/kg, 300-1,200mg/kg.

Phosphatide (PL) of the invention concentrates krill oil, preferably aboard ship (sea) production.So can directly using fresh Raw krill, it is to avoid freezing krill all or in part.Except the freshness of final material mentioned, on ship, production process is also Krill food fresh, that krill oil finally can be further extracted by bank (land) processing factory can be produced.

The method of these preferably aboard ship (sea) production krill oils also has a lot of other important preferential.For example, it is to avoid Raw shrimp will be freezed and be transferred to harbour facilities from offshore activities (on ship), be then transferred to (land) processing factory on the bank.It is of the invention The method of final products is significant in lower cost structure.

Phosphatide of the invention concentrates krill oil, preferably aboard ship (sea) production.This is represented, this method ensure that height is new The use of fresh krill raw in whole or in part, it is to avoid some refrigerating process, cold in original technique that land (bank) implements Freeze the rotten of the phosphatide that occurs in transport and freezing, and/or lipid it is rotten situations such as.

In some replacement concrete operations, process of the invention using only from the marine fresh krill for obtaining, can also not make With the krill of freezing.When this respect is come into effect, it should be noted that avoid or minimize important emulsifying agent of the use containing phosphatide Krill resource.Freezing krill can be defrosted before making.

The present invention illustrates the related environment of strong krill and drives development, and its direct interests includes：

A) the krill seizure amount required for more effectively step causes final products per ton is relatively low.Therefore, with other method Compare, while the output of same final products is ensured, the krill totality seizure amount required for it is relatively low.

B) catch and identical working ability is reduced because of processing on ship.Therefore, during the processing method for being related to other similar, Infrastructure for processing is substantially reduced.

C) the corresponding improvement of the quality of the final products such as freshness and molecular mass, it is meant that ultimate consumer, no matter It is for health or Animal nutrition, it will obtain the interests of the market cost reduction per identical high-quality molecule.

D) present invention ensures relatively low processing cost, and this causes ultimate consumer by by relatively low final products selling price And obtain direct interests.

E) biomass protection equally also ensure that on ship the safety of (sea) value-added concept, nor need extra flower Expense goes to transport the raw material of processing in bank in future (land).

F) present invention allows the safety of (sea) final products on ship.

G) present invention likewise provides more preferable protection of resources.Reason is, seizure be not centered at limited seizure and Process segment rather than whole krill catch season.

The invention further relates to the krill processing of preferably aboard ship (sea) factory's trawler or lash ship.In view of ship Upper processing (on the bank) is processed better than (land) on the bank, present invention relates equally to the krill that (land) processing factory is carried out on the coast Processing.The final editing objective of the processing method that the present invention is announced includes：

A) whole fresh marine organisms are made using non-stirred or method for grinding.

B) decant out the marine organisms of making, such as krill using two-phase decanter, and/or three-phase decanter, and/ Or the solid-liquid separator of any other type, carry out the solid and decanter liquid of fetching portion low fat and dehydration.

D) compressed liquid is extruded to obtain phosphatide concentration marine organisms grease.

E) low fat of part and the decanter liquid of dehydration is obtained in pressing steps b) to obtain neutral lipid concentration ocean Bio-oil and thick juice, for example remaining water is removed from water phase after grease.Thick juice contains substantial amounts of total protein Percentage and other dissolvable krill compositions.

The equipment that this process is used, avoids in whole step or largely avoided stirring (for example, making, inflating Deng), reach the purpose for reducing the oil emulsion containing protein, water and marine organisms phosphatide so that last element is played The effect of emulsifying agent.

(land) processing on ship, including (sea) factory trawler on ship；Or only make processing use or catch and processing Or the lash ship being combined with other effects；Make processing factory's tramper；And/or other marine processing factory's ships；And/or its He carries out the marine processing factory of this specific purposes.Based on above-mentioned processing, by byproduct or final products or both combination The ocean grease of acquisition.

By process of the invention, the fat content of krill food from 5%-15%, protein content from 60%-70%, Moisture is from 6%-10%.

The krill grease synthetic obtained by the present invention, is not exposed to poisonous or nontoxic chemicals in process of production In product.Therefore, according to the food of the food security committee of European Union (EFSA), nutrition and anaphylactogen professional group (Scientific Opinion of the Panel on Dietetic Products Nutrition and Allergies) answer European Union committee member The explanation (The EFSA Journal (2009) .938,1-16) of grease security requirement that can be to being extracted from krill, the present invention Final krill grease can be used safely for the mankind.

The health of krill grease obtained by the present invention is used, be not limited to reduce premenstrual symptoms, preventing hypertension, The health application of control patients blood glucose level, control arthritic symptom, prevention high fat of blood and other modes.This grease can be with The preparation of dermic product, particularly theme or system product are used in, the skin related to necessary fat acid is lacked can be treated Skin disease, such as dry skin, keratodermia, jaundice, acne, corium ulcer, psoriasis, sebum eczema, anaphylaxis skin Scorching and other disease of skin.

Picture brief introduction

Fig. 1 and 2 is the flow chart of the application process.

Fig. 3 is the flow chart for obtaining the abundant krill grease process of neutral lipid consistent with the present invention.

Fig. 4 is the flow chart of synthermal (contherm) process of acquisition krill grease that table 2 below is examined.

The content of the invention is described in detail

1. a kind of method for not using organic solvent to produce krill oil, comprises the following steps：

A) krill is placed in steamer containers and is steamed, the temperature and time of boiling will can ensure that the protein denaturation in krill, And krill is separated into the fluid product and primary stage solids product two parts of primary, and prevent this two parts from producing emulsification to make With；

B) in the case of keeping temperature is not less than 90 DEG C, primary stage solids thing and primary liquid thing are taken from steamer containers Go out；

C) primary stage solids thing and liquids are separated；

D) can not be used during obtaining krill oil, the separation and acquisition from solid product and fluid product respectively Organic solvent.

2. method as claimed in claim 1, krill therein captures in the sea, and described steamed journey is also at sea Carry out.

3. method as claimed in claim 1, the steamed journey is to use the side that indirect steam heating and flowing steam inject What formula was completed.

4. method as claimed in claim 1, by primary stage solids krill product and primary liquid krill product from steamer containers Temperature during middle taking-up is 90 DEG C to 100 DEG C.

5. method as claimed in claim 2, wherein steam carried out in 4 hours after krill captures, and krill exists It is not chilled before steaming.

6. method as claimed in claim 1, steamed journey therein be at one equipped with internal helicoid conveyer and indirectly Carried out on the horizontal steamer containers of steam heater, by the rotationally conveying device by krill from the charging aperture of steamer containers It is sent to discharging opening；

Wherein the rotating speed of Rotatruder is 1-100 per minute turns；

While being steamed using the mode of flowing steam injection steamer containers.

7. the method for claim 1, after krill is steamed, using 3000 turns about per minute or faster water decanter Separated.

8. method as claimed in claim 7, wherein the moment of torsion for separating water decanter used is 1000 to 10000 Newton meter (n.m.)s.

9. methods described as claimed in claim 1, wherein the process that krill oil is extracted from primary stage solids product includes：Will be just Level solid product carries out squeezing and obtains secondary solid product and secondary liquid form product, then secondary liquid form product is carried out into separation must To krill oil and waterborne liquid rich in phosphatide.

10. method as claimed in claim 9, squeezing step therein is completed by a double spiral squeezer, pressure Screw rotating speed is about 2 to 10 turns per minute during squeezing, when toward squeezer conveying, keeps the temperature of primary stage solids product 90 DEG C between 100 DEG C.

11. methods as claimed in claim 9, wherein the separation to secondary liquid product is carried out in whizzer 's.

12. methods as claimed in claim 9, wherein being to the separation method of secondary liquid product：By to secondary liquid Product is centrifuged removal contained humidity so as to obtain the krill oil rich in phosphatide.

13. methods as claimed in claim 1, wherein the step of krill oil is extracted from primary liquid product is included primary Liquids are separated into krill oil and waterborne liquid rich in neutral grease.

14. methods as claimed in claim 10, wherein the separation to primary liquid thing is completed by centrifugal separator.

A kind of 15. krill fluid compositions rich in neutral grease, including：

Weight ratio is 50-100% neutral greases；

Weight is than the DHA and EPA for 2-45%；

Weight is than the phosphatide less than 10%；

Content astaxanthin is 200 to 1500 mgs/kg；

Gross weight is accounted for than 3.4% or more a little less free fatty.

A kind of 16. krill fluid compositions rich in phosphatide, including：

Account for phosphatide of the gross weight than about 30% to 70%；

Account for DHA and EPA of the gross weight than about 10% to 70%；

Account for neutral lipid of the gross weight than about 30% to about 70%；

About 200 to 1500 mgs/kg of the content of astaxanthin；

The content of cephalin accounts for the 1.5% total fat amount of weight at about 1.5 or more of total fat amount；

Lysolecithin accounts for 0.6% or less of total fat amount；And

Free fatty accounts for 3.4% or less of total fat amount；

17. a kind of solid krill products, it is characterised in that the product is according to claim 1 methods described, by squeezing What primary krill solid product production was obtained.

18. a kind of liquid krill oil products, it is characterised in that the krill oil is produced to claim 1 methods described What primary krill fluid product reproduction was obtained.

19. a kind of liquid krill oil products, it is characterised in that the product is given birth to according to method in claim 1 by squeezing What the primary stage solids product of product was obtained.

20. a kind of solid krill meals, it is characterised in that the product is to solid according to production method gained in claim 11 Body product is dried what is obtained.

A kind of 21. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

A) krill is placed in steamer, it is middle at a certain temperature to steam a period of time, make protein denaturation contained by krill, obtain Part degreasing, the primary stage solids product and primary liquid product of dehydration, meanwhile, to avoid primary stage solids product and primary liquid from producing Product form emulsion；

B) in the case of ensureing that temperature is not less than 90 DEG C, by the primary stage solids product and primary of krill from steamer containers Fluid product takes out；

C) the primary stage solids product and primary liquid product are separated；

D) the primary stage solids product that will be separated is squeezed, and is produced so as to obtain the secondary liquid obtained by part squeezing Product and a part of secondary solid product；And

E) the secondary liquid product obtained by squeezing in step d) is centrifuged, so as to obtain not passing through organic solvent The krill fluid composition rich in phosphatide obtained by extracting process.

A kind of 22. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

C) the primary stage solids product and primary liquid product are separated；

D) will be centrifuged according to the primary liquid product obtained by step c), so as to obtain the life of non-organic solvent method Produce krill fluid composition and viscous liquid rich in neutral grease, then by viscous liquid therein selectivity removal.

A kind of 23. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

C) the primary stage solids product and primary liquid product are separated；And

E) the secondary liquid product obtained by the squeezing from step d) is dried treatment, it is molten by non-organic so as to obtain A kind of drying composite containing krill oil that agent method is produced, in the mixture simultaneously containing phosphatide, DHA, EPA, Protein, astaxanthin and neutral grease.

A kind of low krill fluid composition rich in phosphatide of 24. content of beary metal, including krill oil and

A kind of 25. non-organic solvent method production fishes or the method for marine algae extract, comprise the following steps：

A) fish or marine alga or certain part of the two are placed in steamer, a period of time is steamed at a certain temperature, make fish Or contained protein denaturation in marine alga, primary stage solids product and primary liquid product are obtained, meanwhile, to avoid primary stage solids product With primary liquid product formation emulsion；

D) extract of fish or marine alga, but above-mentioned separation are extracted from primary stage solids product and primary liquid product respectively With organic solvent must not be used in extraction step.

Before describing the present invention in detail, the described present invention should be understood to the fortune of the process for being not limited to described in detail herein With.Such invention includes the method that other concrete measures and various sides use.Wording used herein and term are for retouching State, be not used to other kinds of limitation.

Description below is aided with Figs.1 and 2 is reference, will be easier to be understood.

This application provides the system and process of processing marine organisms amount.Krill is the first-selection as marine organisms amount, especially It is krill.The krill or shellfish of other kinds can also system of the invention and process process.For example it is brilliant Krill, E.frigida, E.tricantha, E.vellantini, E.lougirostris, E.lucens, E.similis, E.spinifera, E.recurva, E.pacifica, Thysanoessa macrura, T.vicinia, T.gregaria, T.raschii, T.inermis, Pandalus borealis, Cervimunida johni, Heterocarpus reedi or According to defining herein, krill is preferably able to be processed in the fresh state Pleuroncodes monodom..Additionally, herein The processing of description can from various fishes, and/or algae to obtain grease, extract thing and other useful synthetics.According to above-mentioned Regulation, changes any processing described herein, to substitute krill with fish, and/or algae.

As the results 10 of first-selected krill, Fig.1 is seen.Using traditional approach and instrument, 14,12,10,8,6,5,4, During the seizure krill of 3 or best 2 or 4 hours, fishing boat, lash ship or intermediate processor it is similar or other be adapted to Fish are processed on the ship for implementing processing of the invention.In some concrete operations, krill in 1 hour after being captured or In more preferable 0.5 hour or it is best 20 minutes in, processing on ship krill.In concrete operations of the invention, including ship traction is dragged Net.This trawlnet is for catching krill, and/or reception fishing boat or other krills for processing trawler seizure or other things Kind, preferably received immediately after krill is caught.Trawlnet (the regular fishing tackle being made up of trawlnet, and/or purse seine fishing system Etc.) include the pumping system that the krill that will newly fish for is extracted on ship from trawlnet, it is ensured that processing of the krill in fresh situation.One In a little concrete operations for catching systems, by krill from trawlnet to ship on extract during can be milled and minced, some are just The design of rule trawlnet or pumping system will not cause any destruction (rotten) to this.

In first-selected concrete operations, using conveyer belt or spiral conveyer or helicoidal pump, by fresh krill entirety Or part is sent to producer or makes ship, to avoid stirring krill or emulsified fat containing protein and moisture.Seawater Flowed out from krill, krill is preferably first to be washed with fresh water processing is sent to.

Krill it is whole merely hit with the factory ship of direct, and/or direct steam heating or other heating systems it is processed, For example heat.Can also be using indirectly heat with the injection for directly heating the heating and flowing steam being combined.Culinary art (makes below Used time also includes heating) can be partial or continuous processing.It is preferably used and is cooked to the rotation that steam is directly injected into krill. Also the level culinary art of the rotation of inside can be suitably used.It is low that spiral is so that 1-100rpm, 2-20rpm be more preferable, 5-10rpm is best Speed rotation, emulsified fat is avoided rather than stirring with this.Krill is entered with atmospheric temperature cooks, with least 90 DEG C, possible 90- 100 DEG C, even up to 93-95 DEG C of the temperature in some operations, or not this scope class but to reach protein complete Rotten temperature and leave cookware.Heating krill allows krill to include flowing, and for example the krill through cooking is in order to subsequent Separation is rendered into two-phase：First solid krill part and first liquid krill part.Cooker is not by specific limit System, but based on first-selected operation sequence, in order to avoid emulsified state, cooker does not contain stirring preferably when running.Culinary art phosphorus The practice of shrimp is depending on some but culinary art type these factors for being not limited to use, such as time of batch processing or continuous processing Would is that 10-20 minutes.And the cooking time of Continuous maching has similar time range, these scopes are not causing phosphorus with krill Approximate time in the shrimp phosphatide significantly continuous cooking device of emulsification is consistent.

In some are according to operation, used to avoid grease emulsifying the screw conveyer without krill stirring, helicoidal pump or Other transmission systems, by cooked krill transport to separator or decanter or separation decanter 14, preferably two-phase decantation Device.Can be used any traditional liquid-solid separator that the krill of culinary art is categorized as into the first solid portion and the first liquid portion Point.Reasonably use to whizzers such as decanter separators, suitably accelerate the separation of solid and liquid.It is first at some In the concrete operations of choosing, decanter centrifuge device is used to separate solid and liquid.In concrete measure is substituted, it is possible to use some are such as Level decantation separator etc. substitutes separator.Cooked krill preferably by moment of torsion be 1-10kNn, 1.2-5kNn more preferably, The two-phase decanter that 1.5-3kNm is best, 1.8-2.5kNm is best, speed is 100-10,000rpm, 1 in some operations, More preferably, more preferably, 3,000-4,600r most preferably carries out the dehydration and degreasing of part to 2,000-5,000rpm for 000-8,000rpm, It is isolated to the solid portion and liquid portion of decanter.In some replacement operations, operating speed is in 3.000rpm or more The culinary art krill in decanter high separate.Decanter type does not have and is limited.The moisture of decanter solid is about 40-80%, 50-70% are slightly good, and more preferably, 58-65% is optimal for 55-67%.Table is shown for N ° 1, with relatively low rotary speed (for example 2-10rpm), carry out the processing of direct or indirect heating to krill using helical conveyor, thus obtained decanter solid and The grease and moisture (corresponding to the first solid and the first krill liquid portion respectively) of liquid.The fat content of decanter solid Scope is the wt dry base of 19.8 to 22.4%.Therefore, solid moisture is about the grease of 8% final krill food Content would is that 18.2% to 20.6%.These results represent that simply using two-phase decanter can not obtain preferable, existing The low fat culinary art krill of more krill grease and food again containing the fat content less than 18%.In any case, transported using low speed The fat content and non-emulsified grease of the decanter solid that the cookware for turning is obtained, are less than using what synthermal culinary art was obtained Fat content and non-emulsified grease.The present invention uses low speed rotation cookware, and this can be avoided that emulsification.Additionally, invention process Can cause, be that liquid portion contains more final greases than solid portion.Conversely, rotate at high speed (more than 500rpm) During using equality of temperature degree type cooker, as shown in table 2, emulsion will generally occur, more greases will stay in solid portion and On-liquid part.

The grease and moisture of the decanter solid of table 2. and liquid.Above-mentioned grease and moisture are, equipped with indirect and straight Connect in the cookware of heating system, the krill to being caught in krill fatty acid phase is carried out with low speed rotation helical conveyor Process and obtain.Also reference can be made to Fig.2 flow charts.

Table 1

In some operations of the invention, standardized cookware is used in Continuous maching, its spiral conveyer Low speed rotation speed is preferably 2-10rpm.By said apparatus obtain decanter liquid be not in emulsification, and with pass through The synthermal cooker of tradition obtains decanter liquid phase ratio, and it contains fat content higher.

And then the flow chart of Fig.4, form is shown for N ° 2, when using equality of temperature degree type cooker, decanter solid and The grease and moisture of liquid.The grease for being remained in decanter solid based on a large amount of greases and being stayed in decanter solid The reason in emulsified state, the present invention is not advocated using the device.The krill grease put using repacking is considerably less and final Krill food protein content it is very high, particularly between krill fatty acid phase obtain krill added using the device Work, its protein content is more than 20%.

2., table obtains krill between krill fatty acid phase, the decanter solid obtained using equality of temperature degree type cooker system With the content of grease and moisture in decanter liquid.See Fig.4

Table 2

Although applicant is without being bound by theory, acknowledged is：First-selected decanter described herein and pressure condition Combination so that in culinary art by speed limit rotate device come the formation for reducing emulsification while, in krill grease synthetic The content of triglycerides and phosphatide is also higher.Using the synthermal device of high speed rotating operation, be do not reach it is above-mentioned satisfactory Result.Therefore, low speed rotation speed herein, such as 1-100rpm, 2-20rpm are slightly good, 5-10rpm more preferably, processing phosphorus Stirring is not applied in shrimp, grease emulsifying is avoided with this.

The flow chart of Fig. 2 provides some detailed descriptions on preferred embodiment of the present invention.By cooking and decanting After krill, the decanter liquid containing neutral grease and astaxanthin that will be obtained are put into centrifugal separator, by first time liquid portion It is separated into grease and water.Any traditional Liquid-liquid seperator can be used.Centrifuge can suitably accelerate dividing for liquid From.Seperator can suitably be used.Severe punishment concentration krill grease and the first viscous liquid (flow are obtained by centrifugal separator Viscous liquid 1 in Fig. 1).Oil fraction is put into again more clean centrifugal separator (polishing for confirming in flow charts from Scheming #1), isolate the abundant neutral grease containing astaxanthin with from extra viscous liquid (not representing in flow charts) Krill grease, the phosphatide without (or not detecting).The viscous liquid (viscous liquid 1 in flow chart) of acquisition containing compared with Low protein, most 0.3-0.5%, its not emulsified state is appropriate for being processed further (centrifugation production) and mud.From Heart device can further evaporate viscous liquid.See Fig.3, obtain krill grease processing related to compression solid etc..

In some concrete operations, there is provided obtain the process of the dense oil of neutral lipid.This process include it is independent and Separate equipment：Whizzer, the whizzer for more cleaning, suction pump, pipeline, thermal energy exchange, groove and packing station.Such as This, there is provided comprised the following steps for obtaining the organic solvent lead-free process that krill grease contains composition：

A) a period of time, aboard ship, at a temperature of krill protein content is changed enough krill is made so that part First solid krill carries out the degreasing and dehydration of part, and first liquid krill formation, while avoiding the first solid With the emulsification of first liquid phosphatide.

B) the making ship by first solid of krill and first liquid part from least 90 DEG C is removed.

C) the first solid portion and the first liquid part are separated

D) the first liquid part obtained by step b) is separated, to obtain the nothing containing abundant neutral grease and viscous liquid Solvent organic composition krill oil, it is also possible to optionally therefrom remove viscous liquid.

In the preferred concrete operations of processing of the invention, take out pump or spiral conveyer using spiral or other are not sent out The feeding system of raw stirring, by decanter solids stages or the first solid krill part feeding pressure device 26.Using pressure device, from First solid krill part and the second solid portion squeeze out second liquid krill part.This pressure device is traditional pressure Device, including, such as single pressure device, double helix pressure device or other there is the device of this basic skills.Preferably in continuous process In, it is also possible to being extruded in processing by the gross.The helical pressure device of appropriate suitable treatment and suitable continuous processing by the gross Pressure device with drum pressure device includes：Box pressure device, drum pressure device, pot pressure device, cage pressure device.Certain of concrete measure On the one hand, the first solid portion is extruded using double helix pressure device and the first solid portion for compressing is separated into the second solid-state Part and the second liquid part.By the production line of product of the present invention, using the double helix pressure device with high pressure or other Extrusion system extrudes decanter solid portion, to discharge the grease containing phosphatide being connected with texturising protein.Pressing steps As preferential concrete measure, with the speed of 2-10rpm, or 3-6rpm speed more preferably, 90-96 DEG C of decanter solid provisions temperature Degree, or 93-95 DEG C temperature more preferably, implemented by continuously extruded under the conditions of abundant provisions.Extrusion system is not special Limitation.As described above, decanter solid portion maintains all phosphatide in coagulated protein, the then larger pressure of decanter solid Higher percent, the phosphatide of such as 30%-60% are released to compressed liquid by power.The moisture of compression solid (press cake) contains Amount is about 45-55%, and 48-53% is more preferably.Table 3 was shown in krill fat period, the situation of the different provisions levels of double pressure Under, the grease and moisture of compression solid in the present invention.

Table 3

Comment

Compression solid sample 1-6 is obtained in the case where insufficient decanter solid puts into.

Compression solid sample 7 and 8 is obtained in the case where sufficient decanter solid puts into.

Table shows the synthetic in pressure fluid, the second liquid part and krill fat period for N ° 4.It is public according to institute of the invention The operation and system opened, the fat content of pressure fluid are higher, 3-25%, 5-20% preferably, 8-17% (wet basis number) more preferably, this Depending on seasonal krill fat content and if not emulsification form.Additionally, krill food processing is only applicable double helix pressure Device (rather than preferentially decanter is used).The content of fat is less than 0.5-3% (wet basis number) in compressed liquid, and this depends on krill In content of phospholipid and if not for emulsification state.

Table 4

In an embodiment of the present invention, there is provided a kind of dry compound with phosphatide oil, protein and astaxanthin, should Phosphatide oil contains DHA and EPA, and protein and astaxanthin are obtained in the case where not using seperator to separate by air-drying hydraulic fluid 30 .For most of mankind's health demand levels, related krill of this dry compound 32 equivalent to an individual standard Product demand.Referring to Fig. 2, dry process is carried out under traditional technique using conventional equipment.Matched with it, one Individual step be to obtain the solvent-free organic matter step comprising krill oil ingredient, it is as follows：

A) in a reservoir to krill operation time and temperature be enough to make protein denaturation therein, avoiding solid-state and liquid In the case that state krill part emulsifies, solid-state krill fraction and first liquid krill that first partially skimmed is dehydrated are formed Fraction；

B) first solid-state and liquid krill part are transferred out from container at least 90 DEG C；

C) first solid-state and liquid krill part are separated；

D) the first solid-state krill part separated is compressed, it is partly and a certain amount of with regard to this second solid-state krill of acquisition Squeezed fluid；

E) as the operating result of c) step, squeezed fluid is centrifuged and is dried with obtain one kind have concurrently phosphatide, DHA, The solvent-free dry organic double compound of EPA, protein, astaxanthin and neutral grease.

In embodiment, two-phase decanter bottle and there is twin screw or have a system synergistic working for strong pressure to release Drain the oil the phosphatide of carrying DHA, EPA for containing in fat, and mix astaxanthin and neutral grease.In order to avoid acutely concussion, use Pump or other feedways, the squeezed fluid at a temperature of 25-121 DEG C at a temperature of preferably 50-110 DEG C, preferably exists In 80-100 DEG C of scope, more preferably in 90-96 DEG C of scope of temperature, be drawn into centrifugal treating in separator 34, using helicoidal pump or Other feeding systems, it is to avoid restless, herein, are mixed with the phosphorus of phosphatide, DHA, EPA, protein, astaxanthin and neutral grease 36 Fatty oil is separated.

Separator for Part II liquid being separated into profit is common liquid separating appts, is matched, from Center device is used to accelerate liquid separator.Accelerator in separator is also activated, and in operation, its separator exploitation speed is 4,000-8,000rpm, best automatic cycle are operated under the speed of 4,600-6,800rpm.In any embodiment of the invention In, oil eliminator centrifuge is not particularly limited to, i.e., under conditions of satisfaction is specified, any centrifuge instrument can be used.Phosphorus Shrimp sauce is run with the speed of 5,000-10,000rpm by decontaminating separator, more preferably automatic cycle 6,000-8, Centrifugal treating under the speed of 200rpm, so as to clarified separation.In any of the embodiments of the present invention, purification separation centrifugal device is simultaneously Non-specifically is limited, i.e., any centrifugal device satisfaction is applicable under conditions of specifying.The water 38 separated from separator 34 24 are concentrated in feeding evaporator 22.

As first-selected embodiment, product of the invention, i.e., it is a kind of have phosphatide, DHA, EPA, protein, astaxanthin and in The krill oil of property grease, acquisition be by special special fluid production line, including：One separation sedimentator, one net Separator, pump, pipeline, heat exchanger, fuel tank and a package station, it is entirely different and be different from and other mixed in decanter bottle There is the fluid production line of neutral grease.

Hereafter example 3 and 4, the characteristics of show the krill oil obtained in the present invention：

I) in the present invention, the krill oil product rich in neutral fat is beneficial to man needed for health, and its content includes：

The content of-neutral fat oil is 50100%w/w, preferably 60100% watt/w, most preferably 70100%w/ W,

The content of-DHA and EPA is 245%w/w, preferably 240%w/w, most preferably 535%w/w,

- content of phospholipid is less than 10%w/w, most preferably less than preferably shorter than 5%w/w, 2%w/w,

- content astaxanthin is 2001500 mgs/kg, preferably from 3001200 mgs/kg, most preferably 400 1000 mgs/kg.

II) in the present invention, the krill oil product rich in phosphatide is beneficial to man needed for health, and its content includes：

- total phospholipids content is 30To 70%w/w, preferably 3560%w/w, most preferably 3555%w/w,

- DHA and EPA content are 1070%w/w, preferably 1560%w/w, most preferably 2055%w/w,

- neutral fat content is 3070%w/w, preferably 40%65%w/w, most preferably 4565%w/w,

- content astaxanthin is 2001500 mgs/kg, preferably 3001200 mgs/kg, most preferably 400 1000 mgs/kg.

Produced by technical process of the present invention, two be made up of said components kind fluid product, is to be beneficial to people Class health demand.

From the above, it is contemplated that the component feature of krill oil and the process of this kind of component of acquisition, it can be deduced that such Conclusion, production technology of the present invention is a kind of entirely different, is different from using solvent extraction flow and/or is faced using super Boundary's fluid extraction is fractionated or is a kind of new-type improved production technique by heat fractionation or the production technology of centrifugal treating, and it gives birth to A kind of krill oil with phosphatide, DHA, EPA, protein, astaxanthin and neutral grease is produced.Additionally, with the diet of krill, In any krill season, can there are about 15% highest fat content, about 60% minimum protein content, about 10% Highest moisture.

In embodiment, by the use of antioxidant and/or preservative and/or nitrogen isolation layer, by the life of present disclosure Production. art produces krill oil stable performance.In addition, this kind of krill oil can be stored in plastics or canister, especially hold in stainless steel In device, food grade, pharmaceutical grades and/or the requirement of beauty classes of applications must be met, meanwhile, answer room temperature or refrigeration and lucifuge is protected Deposit.

In embodiment, krill oil provided by the present invention can be applied in dietary supplements and/or health products.This hair The bright pharmaceutical composition and at least one pharmaceutically acceptable biography for also disclosing krill oil of its component including certain effective dose Send agent, excipient, stabilizer, diluent and/or auxiliary addition.In section Example, address krill oil ingredient and protected as light Agent is suitable, and so-called bright protective agent can be used as solarization black frost and/or suntan oil.In section Example, address krill oil and can be used to Enhancing cosmetic industry, says, so-called cosmetics, it is not limited to moisturizing face cream, loose powder, powdery eye shadow, cream matter eye shadow and mouth It is red.In section Example, krill oil ingredient is addressed, blood plasma cholesterol level can be effectively reduced, suppress platelet adhesion reaction, suppressed The formation of atherosclerotic plaque, preventing hypertension controls arthritic symptom, prevents cutaneum carcinoma, strengthens skin breathability, mitigates Premenstrual symptom or control blood sugar level.Additionally, in section Example, health food, pharmaceuticals and toiletry bag are carried containing the present invention The krill oil rich in phosphatide for supplying is also within the scope of the invention.

Embodiment

The present invention is described in detail in conjunction with the embodiments below.The present invention includes but is not limited to following embodiments.

Embodiment 1

The technique that the krill of krill oil and low fat is obtained in the present invention

The one embodiment in the present invention about process is described below.Fig. 2 is the flow of this process Figure.This process is not obviously related to the use of organic matter solvent and/or supercritical fluid carbon dioxide.

Krill is captured in March to May, preferably part in may, in krill meat fertile period, in Austria The fishing ground in gram Buddhist nun archipelago, using pumping system, when capture is to factory's ship container, krill also lives and is dealt with immediately.5th Open form and show that the component of fresh natural whole krill is constituted.

Table 5

Whole krills are focused into venting seawater in sand pit, and are transported in case using belt conveyor, and Stord is used herein Disc Cooker RPH-60 model screw pumps are conveyed at steamer processing.

The operator of following conveyer using indirect steam heating (coming from operator) and scene steam injection (i.e. with Krill live (open) steam is contacted), heated to about 93-95 DEG C with 0 DEG C of krill, the speed of conveyer is preset as 7-8rpm.

The krill for completing immediately using screw pump be extracted into the two-phase decanter of Westfalia CD536 types it is flat in.Connect The operator of conveyer of getting off (is directly steamed using indirect steam heating (coming from operator) and live steam injection with krill Vapour is contacted), heated to about 93-95 DEG C with 0 DEG C of krill, the speed of conveyer is preset as 7-8rpm.The krill for completing Be extracted into immediately two-phase decanter it is flat in.Heating process makes krill enter and maintain and exceedes as a single continuous step 90 DEG C of ideal temperature, the treatment technology unlike including multi source heating and cooling step in the past.

Two-phase decanter bottle is operated with 3100rpm and 2.0kNm, causes a water bottle to be in liquid position phase, at a water bottle In solid-state position phase.1st form shows the liquid of decanter, solid-state position phase composition.

During the liquid phase of decanter bottle is collected into fuel tank under 93-96 DEG C of temperature range, there, it is extracted into screw pump Rotating speed is set in the separation sedimentator of Westfaia SA100 types of 4,600rpm, rotating speed is transported to immediately and is set to 6, In the decontaminating separator of 100rpm, to obtain with the neutral grease but krill oil without phosphatide.During Examples below 3 shows Property fatty oil component and feature.

The solid phase of decanter bottle forms a kind of pressure liquid phase and compression solid under twin-screw extruder.6th form shows The component under both phases is shown.

Table 6

It is less than until compression solid has reached moisture using Atlas Stord Disc Dryer rotary dryers When 10%, compression solid just dries.Examples below 5 shows the krill component obtained from this test.

It is the specific of 4,600rpm that the pressure liquid for being in 93-96 DEG C is mutually extracted into a scheduled rate using screw pump Separate in sedimentator, a scheduled rate is sent into immediately in the specific decontaminating separators of 6,100rpm, phosphorus to be contained to isolate The krill oil of fat.

Shown in the following examples 4 obtained from current experiment with phospholipid moiety close contain DHA and EPA krills The component feature of oil.

Embodiment 2

The flow chart and quantitative analysis of acquisition krill oil and low fat krill powder craft compare in the present invention

Although in the case where no moisture is attached or recovered, the certain natural South Pole of krill of highest fat content Krill, present embodiment shows that in during natural krill is in fat content (estimating 5%) high, production of the present invention The assessment result that the batch production of line is compared.

Following table is in the case where moisture is not attached, in the natural krill side using high lipid content (about 5%w/w) Face, the assessment result that the present invention compares on production technology batch.

Table 7

Table 8

Table 9

Table 10

Table 11

Table 12

Table 13

Table 14

Table 15

Evaporator	Kg/hour
			0

Table 16.

Evaporation drying machine RCD	Kg/hour
			2,032

Table 17.

Table 18

Table 19

Table 20.

Table 21.

Table 22.

Table 23.

Table 24.

DS=solids contents

F=fat contents

M=moistures

MT=tons

Data display in upper table, in process of the present invention, more fat contents is found that in liquid part, therefore also more Easily partly recovered herein.Table 19 is related to content of phospholipid level in pressure liquid phase, the glycerine three of liquid in table 17 and bottle Ester content level is related.

Although in the case where moisture is attached or is recovered, the certain natural South Pole phosphorus of krill of highest fat content Shrimp, table below shows in during natural krill is in fat content (estimate 5%) high, production of the present invention The assessment result that the quantization of line is compared.

Table 25.

Table 26.

Table 27.

Table 28.

Table 29.

Table 30.

Table 31.

Table 32.

Table 33.

Evaporator	Public reprimand/hour
			5.025

Table 34.

Evaporation drying machine	Public reprimand/hour
			2500

Table 35.

Table 36.

Table 37.

Table 38.

Table 39.

Table 40.

Table 41.

Table 42.

Example 3

Krill oil neutral lipid composition of the invention

Table 43

Neutral fat %w/w
	Triglycerides 84.6
Glycerine 4.9
	Free fatty ND
Monoglyceride ND
	Amount to： 89.5
Phosphatide ND ＜ 0.5
	Alpha-tocopherol 0.7
Fatty acid analysis FAME (fatty acid methyl ester) %w/w
	Total sample 8:0 0.0
9:0 0.0
	10:0 0.0
11:0 0.0
	12:0 0.3
13:0 0.0
	14:0 16.3
15.6 0.5
	16:0 18.2
17:0 0.3
	18:0 1.5
19:0 1.0
	20:0 0.0
22:0 0.1
	23:0 0.0
24:0 0.0
	Saturation amounts to 38.3
11:1 0.0
	13:1 0.0
14:1 0.2
	16:1 9.5
17:1 1.2
	18:1cis 14.6
18:1trans 6.4
	20:1 1.2
22:1 0.1
	24:1 0.0

Single unsaturation amounts to 33.2
	18:2 1.3
18:3 (6,9,12) 0.2
	18:3 (9,12,15) 0.6
20:2 0.0
	20:3 (8,11,14) 0.0
20:4 0.0
	20:3 (5,8,11) 0.2
20:5 3.3
	22:2 0.2
22:3 0.0
	22:4 0.0
22:5N3 types 0.1
	22:6 1.1
How unsaturated amounts to 7.1
	Total value all 78.6

ND=is not detected

Example 4

Neutral lipid composition enriches krill oil of the invention

Table 44

Value is the average value of replicate analysis

^*, determined by HPTLC/ density type analyzers

^**, contain pigment.

It is noticeable that the LPC of the mixture reduced levels for therefrom extracting is consistent in step of the invention.Haemolysis Lecithin (LPC) is that phosphatid ylcholine (PC) decomposes the whole extraction process and/or the storage of freezing raw material krill that produce by phase When the general compound that the long-time time limit is constituted.Lower LPC contents can cause the process of very fresh and finite decomposition krill. Being along with a proceeding results of Raw material processing would not be by different deterioration step.This low LPC contents are for we The very unique parameter of invention because our pole fresh feeds process it is fresh in the place in same fishing ground be to shoot RAW krills, work, other operations are extracted from oil on the bank, (but later from the raw material for previously aboard ship having processed in advance Again process on the coast) result of art methods and LPC top grades oil more than 5%.

Total lipid content aliphatic acid is constituted

Table 45

Numerical value is the average value of replicate analysis

The quantitation limit (quantitative limit)=0.06% of fatty acid analysis

Phosphatidic acid synthesis/the lysophosphatidyl choline of aliphatic acid

Table 46

Numerical value is the average value of replicate analysis

The quantitation limit (quantitative limit)=0.06% of fatty acid analysis

The phosphatidic acid composition of aliphatic acid

Table 47

Numerical value is the average value of replicate analysis

The quantitation limit (quantitative limit)=0.06% of fatty acid analysis

The content of triglyceride of aliphatic acid composition

Table 48

Numerical value is the average value of replicate analysis

The quantitation limit (quantitative limit)=0.06% of fatty acid analysis

The sample of the sour fat of particular lipids lipoids composition and aliphatic acid composition, determines high performance thin-layered chromatography (thin Layer) and DR with scanning density quantitatively according to Henderson and Tocher (Henderson, R.J.and Tocher, D.R. (1992) thin-layered chromatography.In lipid analysis：Practical approach (Hamilton, RJ and Hamilton, the U.S., compile in collaboration with), 65- 111, Oxford University Press, Oxford University).

Example 5

Other features of krill oil of the invention rich in phosphatide

Table 49

(^*) calculate repetition values

(^**) calculated according to the Ham methods of modification.(JAOCS 75,1445-1446 (1998)) AOCS suggestion exercises CD1c- 85。

Example 6

The content of beary metal of krill oil of the invention

Table 50

Example 7

The composition of low fat krill meal -- in the krill of southern krill capture

Table 51

Example 8

The use of the krill oil of dietary supplements (health products/meals).

It is supplement essential fatty acid that it describes a fatty foodstuff of richness.This food has been made as biscuit Shape, using krill oil, krill meal or krill dry mixture.

In a bowl, 400 grams of 100 grams of osmanthus lattice oatmeal mixed flours, 500 grams of sugar, 1 egg, this of 150 milliliters Invention krill oil and 10 milliliters of extraction vanilla.Once substantially uniformity, the biscuit of shaping is 25 grams of each weight, and roasting at 160 degree 15 minutes.Cooking time is longer to destroy astaxanthin.

Use will be depending on 18 in the biscuit of dietary amount:2 and 18:Amount of money essential fatty acid needed for 3 grades, and necessary heat Amount.

Example 9

Bright protective agent

Krill oil prepare described in example 3 and 4 can be used for prepare shine black frost and face cream.

A black frost) is shone.

Illustrate two solarization black frost in this example, one with the sun protection factor 5 (SPF5), another with sun protection because 20 (SPF20) of son.

Table 52

B) suntan oil：

The corresponding suntan oil of following explanation, comprising sun-block agent krill oil.

Table 53

Example 10

Use of the cosmetics based on krill oil.

Because krill oil has multiple biological activities, such as EPA and aliphatic acid can be contained as pigment, oxidation resistance Essential fatty acid, can design cosmetics.

A) moisturiser.

Table 54

B) muffin.

It is one elaboration of krill oil based formulation powder adornment of description in this example, wherein comprising 10% (weight/weight Amount).

Table 55

C) eye shadow powder.

It is that the base oil for describing a formula of the invention works out powder eye shadow in this example, wherein comprising 10% (w/w) krill oil.

Table 56

D) eye shadow cream

In this example, the fundamental formular quilt of an eye shadow cream in detail comprising 5.7% (w/w) krill oil of the invention Illustrate.

Table 57

E) muffin.

In this example, the fundamental formular of a muffin in detail comprising 10% (w/w) krill oil of the invention is explained State.

Table 58

F) lipstick.

In this example, the fundamental formular of a lipstick in detail comprising 3% (w/w) krill oil of the invention is explained State.

Table 59

Medical product based on the krill oil of example 11.

Krill and/or offshore oil have been noted can reduce internal cholesterol.It can also suppress platelet adhesion reaction and The formation of bacterial plaque, and reduce vascular endothelial cellular inflammation.It can provide preventing hypertension.It can prevent low-density The oxidation of lipoprotein.Due to increased the inhibitory action of Apolipoprotein B-100 intracellular degradation, it may have a pair of extra-low densities The secretion of lipoprotein.Because it can reduce CIII apolipoprotein Bs, it can also provide the reduction non-lipoprotein of CIII and carry fat egg White B Antithrombin III levels, strengthen the prevention of myocardial infarction.Krill and/or marine oil are adapted to prevention of cardiovascular disease Prevention, using in the relevant coronary artery disease of angiocardiopathy, high fat of blood, hypertension, ischemic disease is (on angina pectoris, the heart Flesh infarct, cerebral ischemia, evidence of the shock without clinical or laboratory ischemic, arrhythmia cordis).

The krill oil capsule of drug ingedient of the present invention composition is containing 1 milliliter of krill oil example 3 and 4 as mentioned.This hair The krill of bright drug regimen mixture thing is dry to include that capsule contains 1 to 5 gram of krill dry mixture.

Claims

A) krill is placed in steamer containers and is steamed, the temperature and time of steaming will can ensure that the protein denaturation in krill, and make Krill is separated into primary liquid product and primary stage solids product two parts, and prevents this two parts from producing emulsification；Between use Steam heating and live steam injection are connect, is heated to 93-95 DEG C with 0 DEG C of krill, the speed of conveyer is preset as 7-8rpm；

B) in the case of keeping temperature is not less than 90 DEG C, primary stage solids product and primary liquid product are taken from steamer containers Go out；

C) primary stage solids product and primary liquid product are separated；

D) can not be used during obtaining krill oil, the separation and acquisition from solid product and fluid product respectively organic Solvent；

The steamed journey is that the mode injected using indirect steam heating and flowing steam is completed；

The process that krill oil is extracted wherein from primary stage solids product includes：Primary stage solids product is carried out into squeezing and obtains secondary solid Body product and secondary liquid form product, then secondary liquid form product is separated the krill oil and aqueous solution for just obtaining being rich in phosphatide Body；

Separation wherein to secondary liquid product is carried out in whizzer.

2. method as claimed in claim 1, krill therein captures in the sea, and described steamed journey is also at sea to carry out 's.

3. method as claimed in claim 2, wherein steam carried out in 4 hours after krill captures, and krill is before steaming It is not chilled.

4. method as claimed in claim 1, steamed journey therein is equipped with internal helicoid conveyer and indirect steam at one Carried out on the horizontal steamer containers of heater, krill is transmitted from the charging aperture of steamer containers by the rotationally conveying device To discharging opening；

Wherein the rotating speed of Rotatruder is 1-100 turns per minute；

5. the method for claim 1, after krill is steamed, using per minute 3,000 turns or faster water decanter by its Carry out step c) separation.

6. method as claimed in claim 5, wherein the moment of torsion for separating water decanter used is 1000 to 10000 Newton meters.

7. method as claimed in claim 1, the squeezing step of wherein primary stage solids product is complete by a double spiral squeezer Into, screw rotating speed is 2 to 10 turns per minute in expressing process, when toward squeezer conveying, keeps the temperature of primary stage solids product Degree is between 90 DEG C to 100 DEG C.

8. method as claimed in claim 1, wherein being to the separation method of secondary liquid product：Enter by secondary liquid product Row centrifugation removes contained humidity so as to obtain the krill oil rich in phosphatide.

9. method as claimed in claim 1, wherein the step of krill oil is extracted from primary liquid product is included primary liquid Product is separated into krill oil and waterborne liquid rich in neutral grease.

10. method as claimed in claim 7, wherein the separation to primary liquid product is completed by centrifugal separator.

A kind of 11. krill fluid compositions rich in neutral grease, method gained according to claim 1, the krill oil combination Thing is obtained in step d) from fluid product, including：

Weight ratio is 50-100% neutral grease；

Weight is than the DHA and EPA for 2-45%；

Weight is than the phosphatide less than 10%；

Content astaxanthin is 200 to 1500 mgs/kg；

Gross weight is accounted for than 3.4% or more a little less free fatty.

A kind of 12. krill fluid compositions rich in phosphatide, method according to claim 1 gained, the krill fluid composition is Obtained from solid product in step d), including：

Account for phosphatide of the gross weight than 30% to 70%；

Account for DHA and EPA of the gross weight than 10% to 70%；

Account for neutral lipid of the gross weight than 30% to 70%；

200 to 1500 mgs/kg of the content of astaxanthin；

The content of cephalin accounts for 1.5% or more of total fat amount；

Lysolecithin accounts for 0.6% or less of total fat amount；And

Free fatty accounts for 3.4% or less of total fat amount.

A kind of 13. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

A) krill is placed in steamer, it is middle at a certain temperature to steam a period of time, make protein denaturation contained by krill, obtain part Degreasing, the primary stage solids product and primary liquid product of dehydration, meanwhile, to avoid primary stage solids product and primary liquid product shape Into emulsion；Using indirect steam heating and live steam injection, heated to 93-95 DEG C with 0 DEG C of krill, the speed of conveyer is pre- It is set to 7-8rpm；

B) in the case of ensureing that temperature is not less than 90 DEG C, by the primary stage solids product and primary liquid of krill from steamer containers Product takes out；

C) the primary stage solids product and primary liquid product are separated；

D) the primary stage solids product that will be separated is squeezed, so as to obtain a part squeezing obtained by secondary liquid product and A part of secondary solid product；And

E) the secondary liquid product obtained by squeezing in step d) is centrifuged, so as to obtain not extracted by organic solvent The krill fluid composition rich in phosphatide obtained by method.

A kind of 14. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

C) the primary stage solids product and primary liquid product are separated；

D) will be centrifuged according to the primary liquid product obtained by step c), so as to obtain the production of non-organic solvent method Krill fluid composition and viscous liquid rich in neutral grease, then by viscous liquid therein selectivity removal.

A kind of 15. methods that krill fluid composition is not produced by organic solvent, are comprised the steps of：

E) the secondary liquid product obtained by the squeezing from step d) is dried treatment, so as to obtain by non-organic solvent side A kind of drying composite containing krill oil that method is produced, contains phosphatide, DHA, EPA, albumen simultaneously in the mixture Matter, astaxanthin and neutral grease.