CN102305854A - Ultrasensitive and quantitative immunochromatographic device and detection method using same - Google Patents
Ultrasensitive and quantitative immunochromatographic device and detection method using same Download PDFInfo
- Publication number
- CN102305854A CN102305854A CN201110204157A CN201110204157A CN102305854A CN 102305854 A CN102305854 A CN 102305854A CN 201110204157 A CN201110204157 A CN 201110204157A CN 201110204157 A CN201110204157 A CN 201110204157A CN 102305854 A CN102305854 A CN 102305854A
- Authority
- CN
- China
- Prior art keywords
- detection
- reaction tank
- rare
- fluorescent nano
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention belongs to the technical field of biomedicine and discloses an immunochromatographic device and a detection method using the same. The immunochromatographic device is composed of an immunochromatographic test strip and a reaction tank. In the immunochromatographic device, quick, ultrasensitive and quantitative detection of a target substance can be achieved by virtue of time distinguishing detection equipment by taking a rare-earth fluorescence nanoparticle as a marker. Compared with the traditional immunochromatographic test strip, the immunochromatographic device has high sensitivity. The device can be widely applied to quick, qualitative and quantitative detection items in the fields of biology, medicine, food safety and the like.
Description
Technical field
The invention belongs to biomedical sector, be specifically related to the immuno-chromatography detection device and the detection method of target substance content in a kind of qualitative and the detection by quantitative sample.
Background technology
Immunochromatography technique is a kind of easy Fast Detection Technique of phase at the beginning of the eighties in last century, and the immuno-chromatographic test paper strip of being processed by this technology consists of the following components usually: backing and stick on sample pad on the backing, pad, cellulose membrane, absorption pad etc.During detection, sample drop is added on the sample pad, sample can move on chromatography strip through capillary action; In the process of migration; With the label generation specific reaction on the pad, produce immune complex, this immune complex continues migration; Combine with the corresponding antigens/antibody generation specificity of surveyed area on the cellulose membrane, form macroscopic detection band.The trace labelling particle that existing immuno-chromatographic test paper strip product is commonly used has nm of gold, nanometer selenium, latex or the like, wherein uses the most extensive with nm of gold.At present, the immuno-chromatographic test paper strip product is used widely in fields such as medical science detection, food security, drug abuses at the most ripe immunochromatography product of disease.
But the following shortcoming of existing immunochromatography product ubiquity: 1) can not detection by quantitative, can only qualitative detection.2) factor causes sensitivity low because the signal of trace particle is weak, reaction is incomplete etc., and having limited it needs application of highly sensitive detection range at some.3) signal is single, is not suitable for joint inspection.
Some REE (as: Eu
3+, Sm
3+, Dy
3+, Tb
3+Deng) can under optical excitation outside, launch bright characteristic fluorescence with the chelate of some sequestrant formation.This type of fluorescence has long, characteristics such as the Stokes displacement big, PLE broad and emission peak are sharp-pointed of life-span.Serving as a mark based on rare earth chelate compound, (Time resolved fluoroisnmunoassay TRFIA) with advantages such as its high sensitivities, is used widely at the medical science detection range for the time-resolved fluorescence analytical technology of thing.The characteristics such as fluorescence lifetime is long except that having, the Stokes displacement is big for the fluorescent nano particle of processing with rare earth chelate compound, PLE broad and emission peak are sharp-pointed, also have outstanding advantages such as stronger, the anti-photobleaching property of fluorescence signal is stronger, good biocompatibility.
Summary of the invention
The object of the invention is intended to the defective to existing immunochromatography technique, and a kind of qualitative and quantitative immuno-chromatography detection device and detection method highly sensitive, easy and simple to handle are provided.
To achieve these goals, the present invention takes three kinds of means to improve the sensitivity of detection: 1) with the fluorescent nano particle thing that serves as a mark; 2) differentiate detection by quantitative equipment with the time and carry out super sensitivity detection; 3) a kind of reaction tank is provided, makes immune response obtain more thoroughly carrying out.
A technical scheme of the present invention is:
A kind of immunochromatographydetecting detecting test strip is provided, and this test strips comprises backing, and on backing, overlaps sample pad, nitrocellulose filter and the absorption pad of pasting successively, wherein is coated with detection zone and reference region on the nitrocellulose filter, also the Quality Control district can be arranged.Reference region is coated with the rare-earth fluorescent nano particle.Fluorescence signal intensity and reference region fluorescence signal intensity ratio through measuring detection zone are realized detection by quantitative.
A kind of reaction tank is provided, and reaction tank contains the compound and the buffer system of rare-earth fluorescent nano particle-antibody/antigen/haptens/other specificity junction mixture matter.Described specificity junction mixture matter is antigen, antibody, the unexpected specificity junction mixture matter of haptens, like Streptavidin, biotin, albumin A, Protein G.
Aforesaid immuno-chromatographic test paper strip is characterized in that reference region is fixed with the rare-earth fluorescent nano particle.
The rare-earth fluorescent nano particle is characterized in that perhaps adsorbing REE (as: Eu with polymeric material or monox parcel as stated
3+, Sm
3+, Dy
3+, Tb
3+Deng) with the chelate that sequestrant forms, this chelate can be launched bright characteristic fluorescence under ultraviolet excitation.
Aforesaid rare-earth fluorescent nano particle is characterized in that grain diameter is between 10nm-10000nm;
Aforesaid reaction tank is characterized in that the rare-earth fluorescent nano particle can be coupled at through modes such as covalent bond, intermolecular force, hydrophobic effect power between antibody/antigen/haptens/other specificity junction mixture matter.
Aforesaid reaction tank is characterized in that wherein containing the buffer system that is fit to biomolecular reaction, and the pH of this buffer system is between 1-13.
Aforesaid reaction tank is characterized in that content is the powder of freeze-drying, can also be liquid.
The present invention also provides a kind of detection method, it is characterized in that the target substance in the sample at first with antigen, antibody or other bound substances fully reaction takes place in reaction tank.
Aforesaid detection method, after it is characterized in that adding sample, antigen, antibody or other bound substances in target substance in the sample and the reaction tank are reacted 0-1h between 15-60 ℃.
Aforesaid detection method, it is characterized in that fully reaction takes place for compound that antibody/antigen/haptens/other specificity junction mixture matter-rare-earth fluorescent nano particle in target substance and the reaction tank forms after, detect with immuno-chromatographic test paper strip again.
The present invention compared with prior art has following technical advantage:
1) replace trace particles such as traditional nm of gold, latex, nanometer selenium with the rare-earth fluorescent nano particle, the preparation immuno-chromatographic test paper strip both had been fit to qualitative detection, can carry out detection by quantitative again.
2) detection sensitivity increases substantially.
3) both be fit to substance and detected, be fit to multiple detection again.
Embodiment
Although content of the present invention is to combine this instance to describe, can not think restriction to patent of the present invention, scope of the present invention is limited appended claims.In addition, those skilled in the art carries out various changes or modification to the present invention in the appended claims restricted portion, and these changes or modified forms belong to protection scope of the present invention equally.
Embodiment 1 rare-earth fluorescent nano particle-antibody coupling matter preparation
1) get the rare-earth fluorescent nano particle that 100 μ L concentration are 10mg/mL (particle diameter: 300nm) in 900 μ L MES (50mM, Ph6.1) in, the vortex mixing;
2) add 20mg NHS, 20mg EDC, vortex mixing;
3) room temperature reaction 30min;
4) the centrifugal 10min of 10000rpm abandons supernatant;
5) the resuspended particle of vortex;
6) add chloromycetin monoclonal antibody, room temperature reaction 2-4h;
7) add isopyknic 2%BSA, continue reaction 2-4h;
8) the centrifugal 10min of 10000rpm abandons supernatant;
9) add the resuspended liquid of 20mL particle (10mM Tris-HCl, 1% skimmed milk power, 5% trehalose), resuspended particle, subsequent use.
The preparation of embodiment 2 reaction tanks
1) according to the amount in 100 μ L/ holes, in 96 hole microwell plates, adds fluorescent nano particle-chloramphenicol antibody conjugate;
2) ℃ freezing 24h-80;
3) fluorescent nano particle in the freeze drying hole-chloramphenicol antibody conjugate;
4) place dry environment to preserve the fluorescent nano particle after the freeze-drying-chloramphenicol antibody conjugate.
The preparation of embodiment 3 immuno-chromatographic test paper strips
The preparation of sample pad: cellulose membrane is cut into the band of 1.5 * 30cm specification, subsequent use;
The preparation of sample pad: nitrocellulose filter is cut into the band of 2.5 * 30cm specification, and at diverse location spraying chloromycetin-BSA conjugate (detection line), sheep anti mouse two anti-(nature controlling line), the fluorescent nano particles (reference line) of NC film, room temperature is dried;
The preparation of absorption pad: thieving paper is cut into the band of 3.5 * 30cm, subsequent use;
The assembling of test strips: absorption pad, nitrocellulose filter, sample pad are attached on the base plate successively, and are cut into the wide test strips of 5mm, 2-8 ℃ of kept dry.
Embodiment 4 detects residual chloromycetin
1) gets 200 μ L fresh milks in reaction tank, slowly blow and beat 10 times;
2) hatch 3min in 40 ℃;
3) sample pad one end with test strips inserts in the reaction tank;
4) continue to hatch 3min;
5) take out test strips, place the time resolution checkout equipment, read the light signal of detection zone, Quality Control district and reference region, the ratio of the fluorescence signal intensity through measuring detection zone and reference region is confirmed the content of target substance in the sample (chloromycetin); Or under ultraviolet light, observe the fluorescence signal on the nitrocellulose filter, fluorescence signal more a little less than, chloramphenicol residue is high more.
Claims (10)
1. an immuno-chromatography detection device comprises immunochromatographydetecting detecting test strip and reaction tank two parts.Said test strips comprises backing, and on backing, overlaps sample pad, nitrocellulose filter and the absorption pad of pasting successively, wherein is coated with detection zone and reference region on the nitrocellulose filter, also the Quality Control district can be arranged.Reference region is coated with the rare-earth fluorescent nano particle.Fluorescence signal intensity and reference region fluorescence signal intensity ratio through measuring detection zone are realized detection by quantitative.Said reaction tank contains the compound and the buffer system of rare-earth fluorescent nano particle-antibody/antigen/other specificity junction mixture matter.
2. like right 1 described immuno-chromatographic test paper strip, it is characterized in that reference region is fixed with the rare-earth fluorescent nano particle.
3. like right 1 described immuno-chromatographic test paper strip, it is characterized in that testing result pot life resolved detection equipment carries out detection by quantitative, also can be used for qualitative detection.
4. like right 1 described reaction tank, it is characterized in that the rare-earth fluorescent nano particle can be coupled at through modes such as covalent bond, intermolecular force, hydrophobic effect power between antibody/antigen/haptens/other specificity junction mixture matter.Described specificity junction mixture matter is antigen, antibody, the unexpected specificity junction mixture matter of haptens, like Streptavidin, biotin, albumin A, Protein G.
5. like right 1 described reaction tank, it is characterized in that wherein containing the buffer system that is fit to biomolecular reaction, the pH of this buffer system is between 1-13.
6. like right 1 described reaction tank, it is characterized in that content is the powder of freeze-drying, can also be liquid.
7. like right 2 said rare-earth fluorescent nano particles, it is characterized in that perhaps adsorbing REE (as: Eu with polymeric material or monox parcel
3+, Sm
3+, Dy
3+, Tb
3+Deng) with the chelate that sequestrant forms, this chelate can be launched bright characteristic fluorescence under ultraviolet excitation.
8. like right 2 described rare-earth fluorescent nano particles, it is characterized in that grain diameter is between 10nm-10000nm;
9. a detection method is characterized in that the target substance in the sample at first with antigen/antibody/haptens/other specificity junction mixture matter fully reaction takes place in reaction tank, carries out immunochromatography again and detects.
10. like right 9 described detection methods, after it is characterized in that adding sample, antigen/antibody/haptens/other specificity substance in target substance in the sample and the reaction tank reacts 0-1h between 15-60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110204157A CN102305854A (en) | 2011-07-20 | 2011-07-20 | Ultrasensitive and quantitative immunochromatographic device and detection method using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110204157A CN102305854A (en) | 2011-07-20 | 2011-07-20 | Ultrasensitive and quantitative immunochromatographic device and detection method using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102305854A true CN102305854A (en) | 2012-01-04 |
Family
ID=45379735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110204157A Pending CN102305854A (en) | 2011-07-20 | 2011-07-20 | Ultrasensitive and quantitative immunochromatographic device and detection method using same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102305854A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103558378A (en) * | 2013-11-07 | 2014-02-05 | 李鑫 | Rapid detection kit for tomato ringspot viruses |
| CN103558379A (en) * | 2013-11-07 | 2014-02-05 | 曹冬梅 | Rapid detection kit for cucumber green mottle mosaic viruses |
| CN103760368A (en) * | 2013-05-03 | 2014-04-30 | 上海溯源生物技术有限公司 | AFP and Free-beta-HCG fluorescence double-label quantitative detection kit |
| CN104090248A (en) * | 2013-12-24 | 2014-10-08 | 上海容晖生物科技有限公司 | Reagent for quantitative detection of Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay |
| CN104655837A (en) * | 2015-02-27 | 2015-05-27 | 南京微测生物科技有限公司 | Fluorescent quantitative test paper strip for simultaneously detecting algal toxins MC-LR/RR/YR and preparation method and application of fluorescent quantitative test paper strip |
| WO2015143830A1 (en) * | 2014-03-27 | 2015-10-01 | 中国科学院福建物质结构研究所 | Dissolution enhancing time-resolved fluorescence immunoassay for rare earth nano material |
| CN105358980A (en) * | 2013-05-10 | 2016-02-24 | 电化生研株式会社 | Assays for identifying compounds that modulate bitter taste |
| CN105548567A (en) * | 2016-01-18 | 2016-05-04 | 武汉菲恩生物科技有限公司 | Kit for time resolution fluorescent quantitative detection on PCT |
| CN107110859A (en) * | 2014-07-24 | 2017-08-29 | 智能指纹有限公司 | Analyzing device |
| CN107367497A (en) * | 2017-08-15 | 2017-11-21 | 华中农业大学 | A kind of biomolecule detection reagent, preparation method, detection device and application |
| CN107643399A (en) * | 2017-03-06 | 2018-01-30 | 广州佰芮慷生物科技有限公司 | A kind of method for improving immune detection system sensitivity and the device using this method |
| CN107688091A (en) * | 2017-09-06 | 2018-02-13 | 上海容晖生物科技有限公司 | A kind of immune quantitative test paper bar of quick detection cyproheptadine and preparation method thereof |
| CN109444413A (en) * | 2018-09-26 | 2019-03-08 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | A kind of detection device |
| CN112014369A (en) * | 2020-08-25 | 2020-12-01 | 上海市皮肤病医院 | System and method for quickly detecting analyte by using ultrasensitive digital chromatography |
| EP3812746A4 (en) * | 2019-08-26 | 2021-09-01 | Jiangsu University | Fluorescent test paper for measuring mercury ions and measurement method |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2511975Y (en) * | 2001-12-08 | 2002-09-18 | 云南大学 | Early diagnostic indicator papers for prostatic cancer |
| CN1646913A (en) * | 2002-04-10 | 2005-07-27 | 反应生物医学公司 | Sensitive immunochromatographic assay |
| CN1645146A (en) * | 2005-02-03 | 2005-07-27 | 厦门大学 | Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip |
| CN1675545A (en) * | 2002-08-27 | 2005-09-28 | 金伯利-克拉克环球有限公司 | Membrane-based assay devices that utilize time-resolved fluorescence |
| CN1784600A (en) * | 2003-05-02 | 2006-06-07 | 安克塞斯生物公司 | Chromatographic assay system |
| WO2006083367A2 (en) * | 2004-11-23 | 2006-08-10 | Response Biomedical Corporation | Immunoassay employing two-step internal calibration reaction |
| CN101120253A (en) * | 2005-01-28 | 2008-02-06 | 持田制药株式会社 | Immunochromatographic test instrument and semiquantitative method using the same |
| CN101261274A (en) * | 2008-02-02 | 2008-09-10 | 王学生 | Method for combining colloidal gold immune leaching and immunity-chromatography for rapidly detecting medicament residue |
| CN101918838A (en) * | 2007-11-22 | 2010-12-15 | 特拉斯医药有限公司 | Method for evaluation of quality of blood sample |
-
2011
- 2011-07-20 CN CN201110204157A patent/CN102305854A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2511975Y (en) * | 2001-12-08 | 2002-09-18 | 云南大学 | Early diagnostic indicator papers for prostatic cancer |
| CN1646913A (en) * | 2002-04-10 | 2005-07-27 | 反应生物医学公司 | Sensitive immunochromatographic assay |
| CN1675545A (en) * | 2002-08-27 | 2005-09-28 | 金伯利-克拉克环球有限公司 | Membrane-based assay devices that utilize time-resolved fluorescence |
| CN1784600A (en) * | 2003-05-02 | 2006-06-07 | 安克塞斯生物公司 | Chromatographic assay system |
| WO2006083367A2 (en) * | 2004-11-23 | 2006-08-10 | Response Biomedical Corporation | Immunoassay employing two-step internal calibration reaction |
| CN101120253A (en) * | 2005-01-28 | 2008-02-06 | 持田制药株式会社 | Immunochromatographic test instrument and semiquantitative method using the same |
| CN1645146A (en) * | 2005-02-03 | 2005-07-27 | 厦门大学 | Immune chromatography with fluorescent rare earth nanometer particle as marker and detecting testing paper strip |
| CN101918838A (en) * | 2007-11-22 | 2010-12-15 | 特拉斯医药有限公司 | Method for evaluation of quality of blood sample |
| CN101261274A (en) * | 2008-02-02 | 2008-09-10 | 王学生 | Method for combining colloidal gold immune leaching and immunity-chromatography for rapidly detecting medicament residue |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760368A (en) * | 2013-05-03 | 2014-04-30 | 上海溯源生物技术有限公司 | AFP and Free-beta-HCG fluorescence double-label quantitative detection kit |
| CN105358980A (en) * | 2013-05-10 | 2016-02-24 | 电化生研株式会社 | Assays for identifying compounds that modulate bitter taste |
| CN105358980B (en) * | 2013-05-10 | 2018-04-27 | 电化生研株式会社 | The preparation of insoluble carrier granular is coloured with the visual field of fluorochrome mark and uses its immunoassay |
| CN103558379A (en) * | 2013-11-07 | 2014-02-05 | 曹冬梅 | Rapid detection kit for cucumber green mottle mosaic viruses |
| CN103558378A (en) * | 2013-11-07 | 2014-02-05 | 李鑫 | Rapid detection kit for tomato ringspot viruses |
| CN104090248A (en) * | 2013-12-24 | 2014-10-08 | 上海容晖生物科技有限公司 | Reagent for quantitative detection of Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay |
| WO2015143830A1 (en) * | 2014-03-27 | 2015-10-01 | 中国科学院福建物质结构研究所 | Dissolution enhancing time-resolved fluorescence immunoassay for rare earth nano material |
| JP2017509902A (en) * | 2014-03-27 | 2017-04-06 | 中国科学院福建物質結構研究所 | Rare earth nanomaterial dissolution / enhanced time-resolved fluorescence immunoassay |
| US10520495B2 (en) | 2014-03-27 | 2019-12-31 | Fujian Institute Of Research On The Structure Of Matter, Chinese Academic Of Sciences | Dissolution-enhanced time-resolved fluoroimmunoassay based on rare earth nanomaterial |
| CN107110859A (en) * | 2014-07-24 | 2017-08-29 | 智能指纹有限公司 | Analyzing device |
| CN104655837A (en) * | 2015-02-27 | 2015-05-27 | 南京微测生物科技有限公司 | Fluorescent quantitative test paper strip for simultaneously detecting algal toxins MC-LR/RR/YR and preparation method and application of fluorescent quantitative test paper strip |
| CN105548567A (en) * | 2016-01-18 | 2016-05-04 | 武汉菲恩生物科技有限公司 | Kit for time resolution fluorescent quantitative detection on PCT |
| CN107643399A (en) * | 2017-03-06 | 2018-01-30 | 广州佰芮慷生物科技有限公司 | A kind of method for improving immune detection system sensitivity and the device using this method |
| CN107367497A (en) * | 2017-08-15 | 2017-11-21 | 华中农业大学 | A kind of biomolecule detection reagent, preparation method, detection device and application |
| CN107688091A (en) * | 2017-09-06 | 2018-02-13 | 上海容晖生物科技有限公司 | A kind of immune quantitative test paper bar of quick detection cyproheptadine and preparation method thereof |
| CN109444413A (en) * | 2018-09-26 | 2019-03-08 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | A kind of detection device |
| CN109444413B (en) * | 2018-09-26 | 2019-08-27 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | A kind of detection device |
| WO2020062490A1 (en) * | 2018-09-26 | 2020-04-02 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | Detection device |
| EP3812746A4 (en) * | 2019-08-26 | 2021-09-01 | Jiangsu University | Fluorescent test paper for measuring mercury ions and measurement method |
| CN112014369A (en) * | 2020-08-25 | 2020-12-01 | 上海市皮肤病医院 | System and method for quickly detecting analyte by using ultrasensitive digital chromatography |
| CN112014369B (en) * | 2020-08-25 | 2024-02-09 | 上海市皮肤病医院 | System and method for ultrasensitive digital chromatography rapid detection of analytes |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102305854A (en) | Ultrasensitive and quantitative immunochromatographic device and detection method using same | |
| CN109633144B (en) | Fluorescence immunochromatography test strip prepared by using aggregation-induced emission fluorescent microspheres as beacon carrier | |
| AU2016267091B2 (en) | Use of fluorescence for the quick and easy determination of S-Adenosylmethionine, S-Adenosylhomocysteine and Homocysteine | |
| CN108080042A (en) | Micro-fluidic chip of binding time resolved fluorometric technology and its preparation method and application | |
| CN106405071B (en) | 25 hydroxy-vitamine Ds3Fluorescence immune chromatography activation fluorescent latex microballoon | |
| Su et al. | Chromatic immunoassay based on polydiacetylene vesicles | |
| CN101526534A (en) | Fluorescence immune chromatography test paper and preparing method and application thereof | |
| CN104169710A (en) | Method and apparatus for time-resolved fluorescence immunoassay testing | |
| KR101597413B1 (en) | High-sensitive lateral flow immunoassay chip using enzyme-mimetic nanoparticles and Methods sensing using thereof | |
| CN105021595B (en) | Quick diagnosis test strips | |
| CN110133281A (en) | CRP and SAA combined detection kit and preparation method thereof | |
| Bian et al. | Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum | |
| CN107271669A (en) | Propepsin, helicobacter pylori antibody and G17 detection method and its kit | |
| CN107328938A (en) | Propepsin and helicobacter pylori antibody detection method and its kit | |
| CN104777315A (en) | Chemiluminescence immunoassay method for detecting S100 based on gold magnetic particles | |
| WO2019088142A1 (en) | Detection agent for bioassay and signal amplification method using same | |
| Wang et al. | Development of an immunochromatographic test to detect white spot syndrome virus of shrimp | |
| CN102288763B (en) | Immunofluorescence method and special kit for detecting ractopamine based on quantum dots | |
| TW200305718A (en) | Polyelectrolytic internal calibration system of flow-through assays | |
| US20160341723A1 (en) | Au nanoparticles encapsulated in nanocompoites and applications thereof in rapid detection of an analyte | |
| CN106645043A (en) | Kit and method for fast quantitatively detecting small molecule compound | |
| CN106526166A (en) | Rapid detection of lean meat powder in pork | |
| KR101612094B1 (en) | Composition for detecting biomarker comprising target-specific probe and detectable labeling agent-antibody composite and using method of the same | |
| CN106645703A (en) | Kit and method for quickly and quantitatively detecting small-molecular compounds | |
| CN108291908A (en) | With immune and the fast and convenient measurement s-adenosylmethionine of chemical method, AdoHcy and homocysteine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120104 |