CN102283315A - Method for producing animal probiotics feed by utilizing compound bacteria-fermented high fiber agricultural and sideline products - Google Patents
Method for producing animal probiotics feed by utilizing compound bacteria-fermented high fiber agricultural and sideline products Download PDFInfo
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Abstract
The invention relates to a method for producing animal probiotics feed by utilizing compound bacteria-fermented high fiber agricultural and sideline products, comprising the following steps of: carrying out impurity removal and smashing on the high fiber agricultural and sideline products, and moistening and steaming after the high fiber agricultural and sideline products are uniformly mixed with auxiliary materials (0-6.0% of ammonia sulphate, and/or 0-3.0% of potassium dihydrogen phosphate, and/or 0-1.0% of calcium chloride and/or 0-1.0% of magnesium sulphate) in proportion; cooling to 20-40 DEG C after steaming is completed, and inoculating robust neurospora and plant lactobacilli, and then carrying out solid state fermentation for 2-5 days at the temperature of 20-40 DEG C; and drying the fermented feed at the temperature of 40-70 DEG C and then smashing to obtain a probiotics feed product rich in carotenoid. The method provided by the invention has the advantages of high production efficiency, low cost and good benefit, pressure of serious shortage of sources of energy feed and protein feed is relieved while the problem of environmental pollution is solved.
Description
Technical field
The present invention be a kind of be raw material with high fiber agricultural byproducts, utilize compound bacteria fermentation production of protein and natural carotenoid content height, low, the nutritious animal probiotic feed of fiber content, belong to farming, secondary product of forestry deep processing field.
Background technology
High fiber agricultural byproducts produce in agricultural product production and process, as rice chaff, rice bran/degrease rice bran, agricultural crop straw, bean dregs, wheat bran, brewex's grains, the tea dregs of rice/cake, rapeseed dregs/cake, stevia rebaudiana dregs, Chinese medicine slag, pomace and Chinese yam skin etc.China is large agricultural country, and the output of high fiber agricultural byproducts is huge, because technical merit is limited, these agricultural byproducts are not utilized effectively always, and utilization rate and economic benefit are low, cause the great wasting of resources, simultaneously environment are also constituted great threat.
Utilize high fiber agricultural byproducts to produce probiotic feed, relevant both at home and abroad research is a lot, mainly be to utilize cellulase producing bacteria, protease-producing strain bacterium and beneficial bacteria of intestinal tract composite fermentation are produced, the bacterial classification of using mainly is a mould, saccharomycete and bacterium three major types, comprise that wood is mould, aspergillus oryzae, aspergillus niger, head mold, candida utili, lactic acid bacteria and bacillus subtilis etc., purpose is intended to agricultural byproducts are utilized again, alleviate the situation that people and animals rob grain, improve the protein content of feed, degraded ANFs wherein improves the bioavailability of feed and the immunity of enhancing livestock and poultry etc.
Sturdy crassa (
Neurospora.crassa), belong to Eumycota, Euascomycetae, the deer horn Zoopagales, excrement shell Cordycepps, crassa belongs to.This bacterium can produce high vigor cellulase, can utilize high fiber agricultural byproducts, as rice chaff, rice bran/degrease rice bran, agricultural crop straw, bean dregs, wheat bran, brewex's grains, the tea dregs of rice/cake, rapeseed dregs/cake, stevia rebaudiana dregs, Chinese medicine slag, pomace and Chinese yam skin etc., but the crude fibre in the efficient degradation raw material becomes compositions such as compound sugar and monose with lignin, improve the palatability of feed, improve dietary protein level, monose and oligosaccharide content, improve the bioavailability of feed etc.Simultaneously, this bacterium can be synthesized the carotenoid of high yield, has peculiar bread fragrance behind the fermenting-ripening, and with some probio, as Lactobacillus plantarum, can symbiosis.This bacteria growing desired nutritional is simple, raw material is easy to get, cost is low, fermentation period is short, the sweat easy operation control, to the higher agricultural byproducts of content of cellulose carry out resource reutilization production added value higher be rich in carotenoid probiotic feed and feed addictive, can improve the utilization rate of resource greatly, have than large economy and social benefit.
Compare with the correlative study and the application of domestic probiotic feed, sturdy crassa is being made no distinction of rank with other strain fermentations aspect the albumen raising rate, but this bacterium is stronger than the adaptability of other bacterial classifications, particularly can utilize the high-cellulose agricultural byproducts to be converted into available Kohlenhydrate, and condition of culture control is more extensive, operate simplyr, this point is that other strain fermentation micro-ecological feeds are incomparable.The high yield carotenoid of this bacterium fermentation is its a big characteristic simultaneously, also at present not find have the collection fiber to decompose in the feed fermentation field, increases percent protein and high yield natural colouring matter bacterial classification.Therefore, the application of this bacterial classification aspect feed fermentation has bigger advantage.
Summary of the invention
The objective of the invention is to utilize the high fiber agricultural byproducts of the compound cooperative fermentation of sturdy crassa and Lactobacillus plantarum, fiber is converted into available Kohlenhydrate, produces protein content and the higher novel probiotic feed that is rich in carotenoid of digestibility, for feed industry provides a kind of production cost low, the production efficiency height, workable, the fermentation technique of high efficiency.
Concrete processing step of the present invention:
(1) high fiber agricultural byproducts are comprised rice chaff, rice bran/degrease rice bran, agricultural crop straw, bean dregs, wheat bran, brewex's grains, the tea dregs of rice/cake, rapeseed dregs/cake, stevia rebaudiana dregs, Chinese medicine slag, pomace and the removal of impurities of Chinese yam skin, pulverization process, promptly 0～6.0% ammonium sulfate and/or 0～3.0% potassium dihydrogen phosphate and/or 0～1.0% calcium chloride and/or 0.～1.0% magnesium sulfate mix in proportion back profit water and steam material with auxiliary material;
(2) be cooled to 20 ~ 40 ℃ after the steaming material is finished, inoculate sturdy vein born of the same parents bacterium and Lactobacillus plantarum simultaneously, 20～40 ℃ of following solid state fermentation 2 ~ 5d;
(3) feed that fermentation is finished places 40 ℃～70 ℃ crushed after being dried, promptly gets the probiotic feed product that is rich in carotenoid.
Main feature of the present invention is a cellulase-producing ability of utilizing sturdy crassa stronger, the crude fibre in the high fiber agricultural byproducts of degrading, simultaneously with the compound cooperative fermentation of Lactobacillus plantarum, improve crude protein in the feed, little peptide, total soluble sugar, compound sugar and contents of monosaccharides, and improve active probiotic quantity in the feed greatly, simultaneously owing to be rich in carotenoid in the ripe spore of thalline, increased natural colouring matter content in the fermented feed, improve its trophic structure, developed a kind of novel probiotic feed.
The invention has the beneficial effects as follows: 1. utilize the high fiber agricultural byproducts of sturdy crassa and Lactobacillus plantarum composite fermentation can reduce crude fiber content significantly, fiber is converted into available Kohlenhydrate, improves its crude protein and carotenoid content, improve feed quality and palatability, improve its digestibility; 2. microbial fermentation probiotic feed, raw material and small investment of production equipment, fermentation time is short, and sweat is easy to control, and the production efficiency height is profitable; 3. utilize the high fiber agricultural byproducts of microbial fermentation to turn waste into wealth, reduce environmental pollution, can alleviate the pressure of protein feeds critical shortage again, can also save a large amount of grains, greatly reduce feed cost, have remarkable economic efficiency and social benefit.
The specific embodiment
Embodiment one:
10～70% rice chaffs, 30～90% rice brans/degrease rice bran and 0.5～4% ammonium sulfate are mixed back profit water steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1.0%, v/w) 4d is cultivated down in 30 ℃ in the back, and 60 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 40% in the finished feed, and the albumen growth rate is 34%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～450mg/kg feed dry weight.
Embodiment two:
0～100% bean dregs, 0～100% rice bran/degrease rice bran and 0.5～3% ammonium sulfate are mixed back profit water steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1.0%, v/w) 3d is cultivated down in 32 ℃ in the back, and 55 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 38%, and active plant lactobacillus number reaches 10
12Cfu/g feed, carotenoid content can reach 380～500mg/kg feed dry weight.
Embodiment three:
10～75% agricultural crop straw powder (straw or wheat stalk etc.), 25～90% rice brans/degrease rice bran and 0.5～4% ammonium sulfate are mixed back profit water steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.0%, w/w) and Lactobacillus plantarum (1.5%, v/w) 5d is cultivated down in 28 ℃ in the back, and 60 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 47% in the finished feed, and the albumen growth rate is 40%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment four:
With 20～75% brewex's grains powder, 25～80% rice brans/degrease rice bran and 1～6% ammonium sulfate, 0.5～3% potassium dihydrogen phosphate, 0.05～1% calcium chloride and 0.02～0.5% magnesium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (3.0%, v/w) 4d is cultivated down in 30 ℃ in the back, and 55 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 42% in the finished feed, and the albumen growth rate is 36%, and active plant lactobacillus number reaches 10
10Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment five:
With 20～85% tea dregs of rice/cake powder (removal Tea Saponin), 15～80% rice brans/degrease rice bran mixes back profit water and steams material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (2.0%, w/w) and Lactobacillus plantarum (3.0%, v/w) 4d is cultivated down in 31 ℃ in the back, and 50 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 38%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment six:
With 20～85% rapeseed dregs/cake powder, 15～80% rice brans/degrease rice bran mixes back profit water and steams material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (2.0%, v/w) 5d is cultivated down in 32 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 40% in the finished feed, and the albumen growth rate is 35%, and active plant lactobacillus number reaches 10
12Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment seven:
With 10～75% stevia rebaudiana dregs, 25～90% rice brans/degrease rice bran and 0.5～4% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (2.5%, v/w) 4d is cultivated down in 28 ℃ in the back, and 50 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 35% in the finished feed, and the albumen growth rate is 30%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 300～350mg/kg feed dry weight.
Embodiment eight:
With 20～85% Chinese medicine ground-slags, 15～80% rice brans/degrease rice bran and 0.5～3% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (2.0%, w/w) and Lactobacillus plantarum (3.0%, v/w) 5d is cultivated down in 30 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 35% in the finished feed, and the albumen growth rate is 30%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment nine:
With 20～80% pomaces, 20～80% rice brans/degrease rice bran and 0.2～4% ammonium sulfate and 0.02～0.5% magnesium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (2.0%, w/w) and Lactobacillus plantarum (3.0%, v/w) 5d is cultivated down in 30 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 35% in the finished feed, and the albumen growth rate is 30%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 320～390mg/kg feed dry weight.
Embodiment ten:
With 20～80% Chinese yam skin powder, 20～80% rice brans/degrease rice bran and 0.5～3% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (2.0%, w/w) and Lactobacillus plantarum (3.0%, v/w) 5d is cultivated down in 32 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 30%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～450mg/kg feed dry weight.
Embodiment 11:
10～70% rice chaffs, 30～90% bean dregs and 0.5～4% ammonium sulfate are mixed back profit water steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1%, v/w) 4d is cultivated down in 36 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 40% in the finished feed, and the albumen growth rate is 38%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 12:
10～75% agricultural crop straw powder (straw or wheat stalk etc.), 25～90% bean dregs and 0.5～4% ammonium sulfate are mixed back profit water steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (2.0%, w/w) and Lactobacillus plantarum (2.0%, v/w) 3d is cultivated down in 30 ℃ in the back, and 65 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 39% in the finished feed, and the albumen growth rate is 35%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 13:
With 20～75% brewex's grains powder, 25～80% bean dregs and 1～6% ammonium sulfate, 0.5～3% potassium dihydrogen phosphate, 0.05～1% calcium chloride and 0.02～0.5% magnesium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (2.0%, v/w) 5d is cultivated down in 32 ℃ in the back, and 65 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 40% in the finished feed, and the albumen growth rate is 34%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 14:
With 20～85% tea dregs of rice/cake powder (removal Tea Saponin), 15～80% bean dregs mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1%, v/w) 4d is cultivated down in 32 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 50% in the finished feed, and the albumen growth rate is 38%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 15:
With 20～85% rapeseed dregs/cake powder, 15～80% bean dregs mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.0%, w/w) and Lactobacillus plantarum (1.5%, v/w) 3d is cultivated down in 35 ℃ in the back, and 55 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 46% in the finished feed, and the albumen growth rate is 32%, and active plant lactobacillus number reaches 10
10Cfu/g feed, carotenoid content can reach 350～450mg/kg feed dry weight.
Embodiment 16:
With 10～75% stevia rebaudiana dregs, 25～90% bean dregs and 0.5～4% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1.0%, v/w) 4d is cultivated down in 32 ℃ in the back, and 45 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 40% in the finished feed, and the albumen growth rate is 38%, and active plant lactobacillus number reaches 10
12Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 17:
With 20～85% Chinese medicine ground-slags, 15～80% bean dregs and 0.5～3% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (1.5%, v/w) 4d is cultivated down in 30 ℃ in the back, and 60 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 35%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～450mg/kg feed dry weight.
Embodiment 18:
With 20～80% pomaces, 20～80% bean dregs and 0.2～4% ammonium sulfate and 0.02～0.5% magnesium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (2.5%, v/w) 4d is cultivated down in 30 ℃ in the back, and 60 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 48% in the finished feed, and the albumen growth rate is 35%, and active plant lactobacillus number reaches 10
10Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 19:
With 20～80% Chinese yam skin powder, 20～80% bean dregs and 0.5～3% ammonium sulfate mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (2.0%, v/w) 4d is cultivated down in 30 ℃ in the back, and 60 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 35%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～500mg/kg feed dry weight.
Embodiment 20:
With 20～85% tea dregs of rice/cake powder (removal Tea Saponin), 15～80% wheat brans mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (1.5%, w/w) and Lactobacillus plantarum (1.5%, v/w) 5d is cultivated down in 31 ℃ in the back, and 50 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 43% in the finished feed, and the albumen growth rate is 40%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 300～350mg/kg feed dry weight.
Embodiment 21:
With 20～85% rapeseed dregs/cake powder, 15～80% wheat brans mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1.0%, v/w) 4d is cultivated down in 32 ℃ in the back, and 55 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 45% in the finished feed, and the albumen growth rate is 39%, and active plant lactobacillus number reaches 10
11Cfu/g feed, carotenoid content can reach 350～400mg/kg feed dry weight.
Embodiment 22:
With 20～90% Chinese yam skins, 10～80% wheat brans mix back profit water and steam material, rub broken after grog is cooled to 20 ~ 40 ℃ rapidly, inoculate sturdy vein born of the same parents bacterium (0.5%, w/w) and Lactobacillus plantarum (1.0%, v/w) 4d is cultivated down in 30 ℃ in the back, and 50 ℃ are drying to obtain the fermentation probiotic feed after the fermentation ends.The crude fibre degradation rate is 50% in the finished feed, and the albumen growth rate is 40%, and active plant lactobacillus number reaches 10
12Cfu/g feed, carotenoid content can reach 350～450mg/kg feed dry weight.
Claims (5)
1. the compound bacteria method that high fiber agricultural byproducts are produced probiotic feed of fermenting is characterized in that:
With the removal of impurities of high fiber agricultural byproducts, pulverization process, promptly 0～6.0% ammonium sulfate and/or 0～3.0% potassium dihydrogen phosphate and/or 0～1.0% calcium chloride and/or 0.～1.0% magnesium sulfate mix back profit water and steam material with auxiliary material;
(2) be cooled to 20 ~ 40 ℃ after the steaming material is finished, inoculate the compound bacteria solid state fermentation simultaneously;
(3) the feed crushed after being dried of fermentation being finished promptly gets the probiotic feed product that is rich in carotenoid.
2. according to the described compound bacteria of claim 1 method that high fiber agricultural byproducts produce probiotic feed of fermenting, it is characterized in that: described high fiber agricultural byproducts comprise rice chaff, rice bran/degrease rice bran, agricultural crop straw, bean dregs, wheat bran, brewex's grains, the tea dregs of rice/cake, rapeseed dregs/cake, stevia rebaudiana dregs, Chinese medicine slag, pomace and Chinese yam skin.
3. according to the described compound bacteria of claim 1 method that high fiber agricultural byproducts produce probiotic feed of fermenting, it is characterized in that: described composite bacteria is sturdy vein born of the same parents bacterium and Lactobacillus plantarum.
4. according to the described compound bacteria of claim 1 method that high fiber agricultural byproducts produce probiotic feed of fermenting, it is characterized in that: described fermentation temperature is 20 ~ 40 ℃, and fermentation time is 2 ~ 5d.
5. according to the described compound bacteria of claim 1 method that high fiber agricultural byproducts produce probiotic feed of fermenting, it is characterized in that: feed fermentation is finished and is placed on 40 ℃～70 ℃ dryings.
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| JP7464244B2 (en) | 2019-11-15 | 2024-04-09 | 国立研究開発法人農業・食品産業技術総合研究機構 | Method for inhibiting fading of carotenoid pigments |
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