CN102283014B - Artificial culture strain of wild edible fungi and culture method of artificial culture strain - Google Patents
Artificial culture strain of wild edible fungi and culture method of artificial culture strain Download PDFInfo
- Publication number
- CN102283014B CN102283014B CN 201110173067 CN201110173067A CN102283014B CN 102283014 B CN102283014 B CN 102283014B CN 201110173067 CN201110173067 CN 201110173067 CN 201110173067 A CN201110173067 A CN 201110173067A CN 102283014 B CN102283014 B CN 102283014B
- Authority
- CN
- China
- Prior art keywords
- strain
- mushroom
- ears
- edible fungi
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 23
- 238000012136 culture method Methods 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 claims abstract description 18
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 34
- 210000005069 ears Anatomy 0.000 claims description 31
- 239000002609 medium Substances 0.000 claims description 23
- 230000001580 bacterial effect Effects 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 241000856147 Pleurotus sp. Species 0.000 claims description 7
- 239000002023 wood Substances 0.000 claims description 7
- 229920000742 Cotton Polymers 0.000 claims description 6
- 238000012364 cultivation method Methods 0.000 claims description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 5
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 229940088417 precipitated calcium carbonate Drugs 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 241000894007 species Species 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 241000222350 Pleurotus Species 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 238000005286 illumination Methods 0.000 description 6
- 239000007640 basal medium Substances 0.000 description 5
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 210000002686 mushroom body Anatomy 0.000 description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
- 235000015278 beef Nutrition 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
- 235000008729 phenylalanine Nutrition 0.000 description 3
- 229960005190 phenylalanine Drugs 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 235000014102 seafood Nutrition 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 239000003147 molecular marker Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 230000002110 toxicologic effect Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000010744 Arachis villosulicarpa Nutrition 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000307222 Clitocybe maxima Species 0.000 description 1
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides an artificial culture strain of wild edible fungi and a culture method of the artificial culture strain. The artificial culture strain of the wild edible fungi is Pleurotussp. P26297; and the collection number of the strain is CGMCC No.4908. By using the Pleurotussp. P26297 as a parent strain, the culture method comprises breeding, fungi growth, fruiting management and fungi picking. The artificial culture strain of the wild edible fungi is obtained by collection of wild saprophytic bacteria and artificial domestication, germplasm resource of the species is protected, a new variety of the edible fungi is developed, the variety structure of the edible fungi is regulated, market variety supply of the edible fungi is enriched, and the strain has relatively high economic and social benefits.
Description
Technical field
The present invention relates to a kind of wild edible fungus artificial cultivation bacterial classification and cultivation method thereof.
Background technology
Wild edible fungus is to be grown in the natural woods, to be in the wild mushroom of wild state fully at natural world, mainly is divided into two classes, saprophytic bacteria and VA Mycorrhizal Fungi, VA Mycorrhizal Fungi also can't manually be cultivated so far, and saprophytic bacteria is to pass through the domestication, and is tame, but also factory culture.The applicant collects a strain wild mushroom from natural forest farm, through after repeatedly taming, now can carry out artificial cultivation.Cultivation strain tentatively predicates Pleurotus according to morphological feature.Because giving birth in its stem, belong to again the macro fungi of Pleurotus, so among the present invention handle in its called after is picked up the ears, after handle was picked up the ears in the self-discovery, through seeking for many years, there are no the discovery report of this bacterium, and artificial cultivation method also had no the bibliographical information of similar research.
Summary of the invention
The object of the present invention is to provide a kind of is sample with wild saprophytic bacteria, through artificial culture, domestication the wild edible fungus artificial cultivation bacterial classification and the cultivation method that form.
Of the present invention picking up the ears (
Pleurotus sp.) P26297, being deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on June 1st, 2011, it is called for short CGMCC, and the address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is: CGMCC No. 4908.
Obvious seafood flavor is arranged after the fruit body maturation of this bacterial classification, detect through the DNA of retrieval edible mushroom illustrated handbook and institute of microbiology of the Chinese Academy of Sciences, confirm that this bacterial strain is a new species of the domestic and international Pleurotus of finding first.
The cultivation method of described wild edible fungus artificial cultivation bacterial classification, with pick up the ears (
Pleurotus sp.) P26297 cultivates for female the kind, sends out bacterium, management of producing mushroom, at last to plucking mushroom.
When adopting test tube to cultivate, mother's kind is inoculated into test-tube culture medium: 4g K
2HPO
4, 2g MgSO
4, 20g agar, the 10g peptone adds water to 1000mL, the pH nature.
When adopting liquid culture, mother's kind is cultivated original seed or cultivated species, original seed or cultivated species are seeded on the planting material for preparing again, the medium that original seed or cultivated species use is liquid nutrient medium: corn flour 40g, sucrose 20g, 4g K
2PO
4, 2g MgSO
4, agar 2g adds water to 1000mL, the pH nature; The planting material C/N ratio is 20-35.
When adopting Bag Material to cultivate, mother's kind is cultivated original seed and cultivated species, cultivated species is seeded on the planting material for preparing again, described planting material is the Bag Material medium: cotton seed hull 48%, wheat bran 18%, wood chip 30%, precipitated calcium carbonate 2%, lime 2%; Above-mentioned moisture content in medium 65-68%; The employed medium of original seed and cultivated species is conventional medium.
Of the present invention picking up the ears (
Pleurotus sp.) P26297 has following characteristic:
1, Toxicological testing:
Toxicological testing is adopted and is given birth to, ripe middle handle is picked up the ears feeding chicken, duck, and the small animals such as dog are tentatively judged middle handle and picked up the ears to be nontoxic spendable edible mushroom.
2, local flavor
The middle handle bright mushroom delicate fragrance of picking up the ears has been boiled one seafood flavor, and mushroom lid mouthfeel is smooth, compares more sustained with mushroom, mushroom handle exocuticle edible, fiber is more, and the mushroom handle is spongy, and mouthfeel is lighter than the mushroom lid, more sustained, dried mushroom has one dense seafood flavor, and the mouthfeel that is used for stewing is sweet.
3, product
Middle handle picks up the ears directly to dry to can be used for list marketing, and the mushroom product are golden yellow, and the mushroom body is larger, and appearance is better, superior in taste; This bacterium contains essential amino acid in eight, and wherein phenyl alanine and tyrosine content surpass mushroom more than 2 times, can be used as raw material of industry source; This bacterium can also as with the germ plasm resource of a kind of high-quality of other classes hybridization of picking up the ears.
Beneficial effect of the present invention:
Wild edible fungus artificial cultivation bacterial classification of the present invention gathers from wild saprophytic bacteria, forms by the domestication; protect the germ plasm resource of these species, developed the edible mushroom new varieties, adjusted the edible fungus variety structure; enriched the kind supply of edible mushroom market, higher economic and social benefit has been arranged.
Description of drawings
Fig. 1 is buddingged in mycelia and the test tube;
Fig. 2 is that bottle is planted fruiting and mushroom volume morphing;
Fig. 3 is the spore electron-microscope scanning;
Fig. 4 is the mushroom body characteristics of soil fruiting and different times.
Embodiment
Of the present invention picking up the ears (
Pleurotus sp.) P26297 is that the wild saprophytic bacteria that gathers natural forest farm does sample, adopts tissue isolation to form through domestication.
In the domestication process, the bacterial classification that separates is placed on (different C/N ratios, temperature, pH, illumination etc.) bacterium cultivation, fruiting under the various environment, mycelial growth is dense, the later stage is linear thereby filter out, and the more stable bacterial strain of heritability is done female the kind.Concrete screening process is as follows:
Tissue is separated the sparse mycelia that obtains, carry out many cultures under different temperature, find that mycelial growth rate is the fastest under 22 ℃ of conditions, mycelia is sturdy.By the screening of several kinds of carbon source, nitrogenous source, add basal medium with peptone 1%.For the examination carbon source be: maltose, starch, lactose, fructose, sucrose, glucose.Their additions in the 1000mL medium are equivalent to the total carbon of 20g glucose.Add basal medium with glucose 2%.For the examination nitrogenous source be: yeast extract, beef extract, peptone, NH
4NO
3, NH
4Cl, KNO
3Their additions in the 1000mL medium are equivalent to the total nitrogen content of 10g peptone.To respectively make 6 kinds of medium with the basal medium combination respectively for 6 kinds of Carbon and nitrogen sources of examination, place 22 ℃ of constant incubator continuous culture 10d, survey colony diameter, find this bacterium take glucose as carbon source, growth is ideal in the medium take peptone as nitrogenous source; Take glucose as carbon source, peptone is that nitrogenous source adds basal medium, C/N is adjusted to 10/1,15/1,20/1,25/1,30/1,35/1,40/1,45/1,50/1,60,1 totally 13 kinds of prescriptions, place 22 ℃ of constant incubator continuous culture 10d, survey colony diameter, observe the mycelia growing way.C/N is 30 o'clock, and the mycelia amount reaches maximum.By the common miscellaneous bacterias such as mould, green mold, wood is mould are carried out the resistance test, filter out the wherein bacterial strain of resistance.Bacterial strain and mushroom, flat mushroom, the clitocybe maxima that screens carried out antagonistic effect, find that the pleurotus edible fungus of this bacterium and test all produces antagonism.Under different intensities of illumination and light application time, induce the mycelia kink to form the mushroom flower bud, as shown in Figure 1, the mushroom flower bud forms the mushroom body of fine strip shape along with growth.Add 30mL medium (for the above-mentioned medium that screens) and make flat board in the 150mL triangular flask, 1 access of bacterium colony with aseptic card punch cut-off footpath 5mm places 22 ℃ of constant temperature dark culturing 25d.After mycelia is covered with flat board fully, place respectively complete darkness, 12h light (3000lx) secretly alternately, continuous culture 15d under continuous illumination (3000lx) condition, observe the record number of buddingging, find that small mushroom bud appears in test tube under the above illumination of 12h.Small mushroom bud is organized separation, obtain new bacterial strain.The method that adopts traditional sorting technique to combine with the dna molecular marker technology, be foundation according to morphological feature, comprise the microstructure of the type of morphological feature, mycelia of fruit body and structure, spore, the microstructure of fruit body etc. tentatively predicate Pleurotus.By dna molecular marker, be defined as Pleurotus again.The foundation that middle handle picks up the ears to name is according to giving birth in the stem, belonging to again the macro fungi of Pleurotus, so handle is picked up the ears in the called after, and it is carried out culture presevation.
Do female the kind with above-mentioned bacterial classification, cultivate original seed and cultivated species, again cultivated species is seeded on the planting material for preparing, through sending out a bacterium, management of producing mushroom, at last to plucking mushroom.With female raw materials such as employed employing cotton seed hulls when cultivating original seed and cultivated species, cow dung powder, wood chip of planting, by different ratio proportionings, the handle plant formulation of the good original seed of mycelial growth, cultivated species and cultivating superior high-yield of picking up the ears in the screening.The handle supporting culture medium prescription of picking up the ears in the discovery, test-tube culture medium is: 4g K
2PO
4, 2g MgSO
4, agar 20g, 1% peptone, water 1000mL, pH nature; Liquid nutrient medium: corn flour, 40g, sucrose 20g, 4g K
2PO
4, 2g MgSO
4, agar 2g, water 1000mL, pH nature; Cultivating in bag medium: cotton seed hull 48%, wheat bran 18%, wood chip 30%, precipitated calcium carbonate 2%, lime 2%; Water content 68%.Pick up the ears the mycelia growth and development to the requirement of temperature and the climatic characteristic of this area according to middle handle, the handle rational cultivation season of picking up the ears in the selection, the sowing time in spring is arranged in February 15, February 28, March 15, March 31; The Summer-sowing arrangement of time is in July 15, July 31, August 15, August 31.The performance of mushroom body different growing stage as shown in Figure 4, middle handle is picked up the ears dry product through country's processed food quality supervision and test Spot detection, the result is as follows: total reducing sugar 43.1%, total fatty acids 1.2%, asparatate 17.0g/kg, serine 8.1 g/kg, glutamic acid 23.7%, glycine 7.1 g/kg, histidine 2.8 g/kg, arginine 6.4 g/kg, threonine 7.4 g/kg, alanine 9.5 g/kg, proline 5.8 g/kg, cystine 0.9 g/kg, trorsine 14 .3 g/kg, valine 7.2 g/kg, methionine 1.7 g/kg, lysine 7.8 g/kg, isoleucine 5.7 g/kg, leucine 9.3 g/kg, phenyl alanine 6.0 g/kg, and with the common high-quality edible mushroom such as mushroom relatively.Phenyl alanine in the essential amino acid that handle is picked up the ears in the discovery, tyrosine surpass mushroom more than 2 times.And by this edible mushroom is detected four kinds of Heavy Metals adsorption capacities, find that this edible mushroom all reaches nuisanceless level to four heavy metal species residual quantity.
Pick up the ears (
Pleurotus sp.) form, the biological property of P26297 mycelia on medium:
The mycelia form as shown in Figure 1, and is similar to flat mushroom, is pure white, dense, aerial hyphae at agar medium.The middle handle fruit body of picking up the ears is sturdy, scattered, and 5～18 centimetres of bacteria cover diameters just are umbrella shape, becomes gradually the edge wave shape wave at last for open and flat, as shown in Figure 4.
The domestication that middle handle is picked up the ears cultivates can adopt following medium: with 4g K2PO4,2g MgSO4,20g agar, 1000mL water, the pH nature is as basal medium, contain in every liter of medium: 20g maltose, starch, lactose, fructose, sucrose, glucose are as carbon source, contain in every liter of medium: 10g yeast extract, beef extract, peptone, NH4NO3, NH4Cl, KNO3 are as nitrogenous source, the following medium of preferred employing: 20g glucose, 10g beef extract, 4g K2PO4,2g MgSO4,20g agar, 1000mL water, pH nature.The C/N ratio that middle handle is picked up the ears can normally be produced between 10-60, preferred 25-40, and optimum C/N ratio is 30.The middle handle mycelia of picking up the ears can be cultivated under 5-35 ℃ of temperature, and killing temperature is 45 ℃ of 20min, and preferred 22 ℃, cultivate 10d at 22 ℃, it is maximum that the mycelia amount reaches.The middle handle mycelia of picking up the ears can be cultivated under the pH4-10 condition, preferred pH5-6, and optimum value is 6.0.Middle handle is picked up the ears spore can be 10-30 ℃ of sprouting, and preferred 15-25 ℃, optimum Germination Temperature is 25 ℃.Middle handle is picked up the ears and can be buddingged under 8-24h illumination, and complete darkness can't be buddingged, and optimum is continuous illumination 12h.The stalks such as the cultivation that middle handle is picked up the ears can wood chip, straw, Caulis Miscanthis floriduli or wooden processing factory offcuts are as carbon source, and as carbon source, C/N ratio is 20-35 with cow dung, cotton seed hulls, wheat bran, groundnut cake etc., and are preferred 30, add a small amount of lime, precipitated calcium carbonate etc.Optimum medium culture is: cotton seed hull 48%, wheat bran 18%, wood chip 30%, precipitated calcium carbonate 2%, lime 2%; Water content 68%.Middle handle picks up the ears to cultivate fruiting can be at 16-35 ℃ of normal fruiting, and preferred 20-28 ℃, optimum fruiting temperature is 25 ℃.
Claims (2)
1. wild edible fungus artificial cultivation bacterial classification is characterized in that: described wild edible fungus artificial cultivation bacterial classification for pick up the ears (
Pleurotus sp.) P26297, preserving number is CGMCC No.4908.
2. the cultivation method of a wild edible fungus artificial cultivation bacterial classification as claimed in claim 1 is characterized in that: with pick up the ears (
Pleurotus sp.) P26297 cultivates for female the kind, sends out bacterium, management of producing mushroom, at last to plucking mushroom; Its supporting culture medium prescription, test-tube culture medium is: 4g K
2PO
4, 2g MgSO
4, agar 20g, 1% peptone, water 1000mL, pH nature; Liquid nutrient medium: corn flour 40g, sucrose 20g, 4g K
2PO
4, 2g MgSO
4, agar 2g, water 1000mL, pH nature; Cultivating in bag medium: cotton seed hull 48%, wheat bran 18%, wood chip 30%, precipitated calcium carbonate 2%, lime 2%; Water content 68%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110173067 CN102283014B (en) | 2011-06-24 | 2011-06-24 | Artificial culture strain of wild edible fungi and culture method of artificial culture strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110173067 CN102283014B (en) | 2011-06-24 | 2011-06-24 | Artificial culture strain of wild edible fungi and culture method of artificial culture strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102283014A CN102283014A (en) | 2011-12-21 |
| CN102283014B true CN102283014B (en) | 2013-01-02 |
Family
ID=45330008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110173067 Expired - Fee Related CN102283014B (en) | 2011-06-24 | 2011-06-24 | Artificial culture strain of wild edible fungi and culture method of artificial culture strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102283014B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104584866B (en) * | 2015-02-05 | 2017-02-22 | 莆田市农业科学研究所 | Off-season cultivation method of wild edible fungus |
| CN105948938B (en) * | 2016-06-08 | 2019-09-24 | 辽宁省微生物科学研究院 | A kind of synthetic media of edible mushroom and its preparation method and application |
| CN109220514B (en) * | 2017-05-23 | 2021-03-23 | 中国科学院微生物研究所 | Separation and artificial domestication cultivation method of new wild edible fungi |
| CN109362482A (en) * | 2018-12-14 | 2019-02-22 | 大连森源菌业有限公司 | A kind of High Yielding Cultivation of Pleurotus ostreatus method |
| CN110622777A (en) * | 2019-10-23 | 2019-12-31 | 江苏农林职业技术学院 | Culture medium of morchella mother strain and preparation method of morchella mother strain |
| CN111990167A (en) * | 2020-07-31 | 2020-11-27 | 中南林业科技大学 | Oyster mushroom culture medium and preparation and use methods thereof |
| CN114480144A (en) * | 2022-03-17 | 2022-05-13 | 石家庄原动力工业技术有限公司 | Artificial cultivation method of wild edible fungi |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878719A (en) * | 2009-05-05 | 2010-11-10 | 何寒 | High-yield and fast cultivation technology of pleurotus edible fungus |
-
2011
- 2011-06-24 CN CN 201110173067 patent/CN102283014B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878719A (en) * | 2009-05-05 | 2010-11-10 | 何寒 | High-yield and fast cultivation technology of pleurotus edible fungus |
Non-Patent Citations (2)
| Title |
|---|
| 王谦 等.黄白侧耳栽培条件优化.《食用菌学报》.2010,(第4期),第26-29页. * |
| 郭惠东 等.白灵侧耳优质高效栽培关键技术研究.《中国食用菌》.2006,第25卷(第3期),第23-25页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102283014A (en) | 2011-12-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102283014B (en) | Artificial culture strain of wild edible fungi and culture method of artificial culture strain | |
| CN106034742B (en) | A method of clitocybe maxima is produced using mulberry bar, bagasse and silkworm excrement | |
| CN100549158C (en) | A kind of campelyco and preparation method thereof | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| Gaitán-Hernández et al. | Obtaining and characterizing Pleurotus ostreatus strains for commercial cultivation under warm environmental conditions | |
| CN102668885B (en) | Pholiota adipose new strain and method for cultivating fruiting body of pholiota adiposa new strain | |
| JP2013226130A (en) | New ganoderma neo-japonicum strain and method of artificial cultivation of the same | |
| CN101491195A (en) | Phlebopus portentosus cultivation method | |
| CN106613349A (en) | Tremella aurantialba substitute cultivation method | |
| JP2009240241A (en) | New strain of pleurotus ostreatus and method for artificially cultivating the same | |
| CN103468591B (en) | Salt-tolerant trichoderma pleuroticola strain and application thereof | |
| CN109749953B (en) | Bacillus cereus, microbial inoculum and preparation method and application thereof | |
| KR101305243B1 (en) | A novel strain pleurotus nebrodensis and method of producing it | |
| KR101312059B1 (en) | A novel strain pleurotus nebrodensis | |
| Priya et al. | Biology and cultivation of black ear mushroom–Auricularia spp | |
| CN104498363A (en) | Growth promoting endophytic beauveria bassiana strain and application thereof | |
| CN107641600A (en) | Suitable for the flat mushroom JK02 bacterial strains and its cultural method of low temperature fruiting and application | |
| CN101849478B (en) | Coprinus atramentarius and cultivation method thereof | |
| CN108770593B (en) | Lepista nuda strain and fruiting body cultivation method thereof | |
| Kumara et al. | Improvement of strains of two oyster mushroom cultivars using duel culture technique | |
| CN110240528A (en) | A kind of microbial organic fertilizer and its application | |
| CN111849842B (en) | Potassium bacteria, potassium bacteria microbial inoculum comprising same and application | |
| CN112673900B (en) | A strain of Rumex crispus and its cultivation, picking and preservation method | |
| CN106222095B (en) | A kind of heat resistance fungus novel species and its application in microbial manure | |
| CN110205258B (en) | Streptomyces bacterial strain PBS9 for preventing and treating potato scab and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: DEZHENYUAN NEW AGRICULTRIAL (LIANYUNGANG) CO., LTD Effective date: 20150115 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Zhengming Inventor after: Yao Yijian Inventor after: Peng Jianping Inventor after: Li Biqiong Inventor after: Lin Junyang Inventor after: Qiu Chunjin Inventor after: Zou Yongqian Inventor before: Chen Zhengming Inventor before: Yao Yijian Inventor before: Peng Jianping Inventor before: Li Biqiong Inventor before: Lin Junyang Inventor before: Qiu Chunjin |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: CHEN ZHENGMING YAO YIJIAN PENG JIANPING LI BIQIONG LIN JUNYANG QIU CHUNJIN TO: CHEN ZHENGMING YAO YIJIAN PENG JIANPING LI BIQIONG LIN JUNYANG QIU CHUNJIN ZOU YONGQIAN |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150115 Address after: 351144 Putian city of Fujian Province, Huangshi Town, Licheng District, Putian Province Patentee after: PUTIAN AGRICULTURAL SCIENCE INSTITUTE Patentee after: DEZHENYUAN NEW AGRICULTURAL (LIANYUNGANG) Co.,Ltd. Address before: 351144 Putian city of Fujian Province, Huangshi Town, Licheng District, Putian Province Patentee before: Putian Agricultural Science Institute |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |