CN102257922A - Novel production and deep processing technology for cordyceps - Google Patents
Novel production and deep processing technology for cordyceps Download PDFInfo
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Abstract
The invention discloses novel production and deep processing technology for cordyceps, belonging to the novel technical fields of biochemistry and the like. According to the adopted technical scheme, the novel production and deep processing technology for cordyceps comprises the following steps of: I, preparing a mother culture; II, transforming the mother culture into an original culture and culturing; III, preparing a suspension liquid strain; IV, preparing a liquid strain; and V, manually culturing a cordyceps strain. The invention has the advantages that: by adopting the novel production and deep processing technology for cordyceps, the yield of Chinese caterpillar fungus can be increased, the price of the Chinese caterpillar fungus can be lowered radically, the pain of diseases is relieved for patients, and another wonderful work is increased for the conventional medicinal library in China.
Description
Technical field
The invention belongs to new technical field such as biochemical industry, production of especially a kind of Cordyceps militaris and deep working technology.
Background technology
Over 100 years, Chinese traditional Chinese medicine circle is spreading always: " Chinese medicine three is big precious, genseng, pilose antler, CORDYCEPS ".Cordyceps sinensis is a kind of flat tonifying yin resistance, rare traditional Chinese medicine with very high health, health care and medical value, it contain many kinds of human body needs, all irreplaceable nutrient component of other any animals and plants, property of medicine gentleness, equal throughout the year edible, the old or young, sick, weak, virtual person is all suitable.Modern medicine is discovered: Chinese caterpillar fungus is rich in cordycepic acid, cordycepin, amino acid, sterol, mannitol, alkaloid, vitamin B1, B2, polysaccharide and mineral matter etc., can improve body's immunity, not only the function to the various internal organs of human body has regulating action, also has some direct disease-resistant function.
Cordyceps sinensis is also named Cordyceps militaris, belongs to Ascomycotina, Clavicipitaceae, Cordyceps, is a kind of medicinal fungus of preciousness.Be the distinctive class valuable ingredient of traditional Chinese medicine of China, have high value medical health care and add economic worth, at home and abroad enjoy high reputation, have the laudatory title of " the legendary jewellery in east ", be the traditional foreign exchange earning commodity of China, so Cordyceps sinensis is a wonderful work in China's traditional medicine treasure-house.
Cordyceps sinensis is grown in the high and cold area in China western part and the west and south, growing environment is special, resource-constrained, along with further investigation both domestic and external for many years, further recognize medical treatment and the nourishing research of Cordyceps sinensis to human body, further recognize the important value that Cordyceps sinensis is had the medical treatment of human body and nourishing, demand constantly increases, because the natural cordyceps resource is originally just few, adds that worm grass resources quantity falls sharply, output constantly descends, disparities between supply and demand are increasingly sharpened, cause the market supply and demand to disconnect, cost an arm and a leg
So it is with short production cycle that people begin to cultivate; there is the Cordyceps militaris of similar medicinal health care function to satisfy consumption demand to natural cordyceps; substitute the deficiency of natural cordyceps, for the development of the Cordyceps militaris of artificial large-scale cultivation provides vast market and good development opportunity.To the artificial introduction and acclimatization of Cordyceps sinensis, among the research work of aspects such as cultivation heredity and application,, open up medicine source biofermentation technique and have great value and development prospect really as a kind of new technology new technology that development potentiality is arranged.
Summary of the invention
The purpose of this invention is to provide a kind of production and deep working technology that can replace Cordyceps sinensis to be used as medicine, can to replace the Cordyceps militaris in natural cordyceps medicine source.
Technical scheme of the present invention is: production of Cordyceps militaris and deep working technology comprise the following steps: that (one) female kind prepares; (2) the female kind changeed original seed and cultivated species: the preparation of (three) suspension bacterial classification, the preparation of (four) liquid spawn; (5) artificial cultivation of Cordyceps militaris spawn.Detailed step is as follows:
One, the female kind prepares
The female kind prepares employing spore separation method, belongs to the sexual propagation method.It is to utilize the mushroom fruit body to launch the principle of spore voluntarily under aseptic condition, and spore is scattered on the synthetic medium, by cultivating, obtains original fungus strain body, and then moves the access slant tube and cultivate.Described female kind prepares and comprises that (1) makes medium: the female kind of (2) test tube prepares: the enlarged culture of the female kind of (3) test tube; (4) go out the grass test; (5) separate tissue is made female the kind.
Two, the female kind changeed original seed and cultivated species
Original seed and cultivated species derive from female expansion of planting, and original seed is the natural cs bacterial strain, after artificial training, propose the high-quality bacterium colony and change solid culture medium over to, obtain the one-level mother through different temperature, humidity, nutrients of each stage and plant.Female kind can only be made bacterial classification and use, and must not directly cultivate, and cultivates so must make the original seed tube, and general original seed also is difficult for directly cultivation, because the original seed source is few, the cost height needs repeatedly tube to cultivate into cultivated species.Female kind changes original seed and cultivated species comprises: (1) culture medium preparation, (2) female tube of planting enlarge.
Three, the preparation of suspension bacterial classification
Suspension belongs to the category of liquid spawn, but being suspended in can only be the dilution of female kind and original seed to a great extent, and liquid spawn is only the rejuvenation breeding of Chinese caterpillar fungus, the suspension effect is not as liquid spawn on identical consumption, the suspension bacterial classification is made fairly simple, invest lessly, the cultivation person of lacking experience can use the suspension manufacturing technology earlier, and the bacterial classification consumption is greatly a bit to improve the infection success rate when just cultivating.Described suspension strain preparation comprises: the preparation of (1) medium, (2) sterilization, (3) inoculated and cultured.
Four, liquid spawn is made
Adopt the single-stage cultivation to be liquid production of hybrid seeds express method again, progressively realize multistage cultural method, realize that the stable high yield cycle short, FFI high-efficiency strain.Single-stage is cultivated with short production cycle, can turn out first Chinese caterpillar fungus liquid spawn in 8-10 days, output is bigger, can produce 1000-2000 milliliters of liquid bacterial classification as required for 1 time, and can produce continuously, cultivation is convenient, can to send out bacterium fast as long as liquid spawn is sprayed onto media surface, be solid send out the bacterium time half, the output height, be 2 times of solid bacterium output, and fundamentally having solved infection time, the success rate height has been stopped assorted bacterium fully with special filter, saccharomycetic infection, success rate needs 6-8 month time up to more than 95% with conventional method plantation Chinese caterpillar fungus, adopts liquid spawn, only need 50-60 days, shortened the Chinese caterpillar fungus production cycle greatly.Described liquid spawn is made and comprises (1) liquid spawn culture medium prescription, (2) inoculated and cultured.
Five, Cordyceps militaris artificial cultivation, described Cordyceps militaris spawn artificial cultivation comprises: (1) is pressed culture medium prescription and is made medium, the bottling of (2) spice; (3) sterilization; (4) inoculation; (5) culturing room cultivates: (6) final-period management; (7) gather.
Effect of the present invention is: by production and the deep working technology of Cordyceps militaris of the present invention, can increase the output of Cordyceps sinensis, fundamentally reduce the price of Cordyceps sinensis, the misery that palliates a disease for extensive patients plays a role, for increasing another wonderful work again in China's traditional medicine treasure-house.
Embodiment
The present invention is described further below in conjunction with embodiment.
Production of Cordyceps militaris and deep working technology comprise the following steps:
One, the female kind prepares, and comprises the following steps:
(1) makes medium
Medium can select following two kinds of prescriptions to make: oats flakes adds water, and heating 1 hour is supplied amount of preparation with adding water after the filter of two-layer gauze in the boiling water-bath, adds the incubator sterilization of packing into after the agar in-depth.
(2) the female kind of test tube prepares
1, the enamel basin of about 25 centimetres of diameters, 4 layers of gauze of last lining, on gauze, put a medium size incubator (medium is housed in the device), and in device, put a stainless steel tripod, for inserting Chinese caterpillar fungus stroma usefulness, above incubator, add a cover a glass bell jar, bind up with gauze together with porcelain dish, sterilization is 1 hour under 147 kpa pressures, and the cooling back is standby.
2, choose fresh, high-quality, maturation, as yet do not distribute the normal Chinese caterpillar fungus stroma of spore clean with flushing with clean water, dry.
3, the package unit with the above-mentioned bacterium of going out removes gauze, put into the inoculating hood strict sterilization together with cordyceps militaris sporocarp 0.1% mercuric chloride liquid (or 75% alcohol) sterile water etc., after the sterilization, under aseptic condition, to the cordyceps militaris sporocarp surface sterilization, and, take bell jar then apart with 0.1% mercuric chloride liquid (or 75% alcohol) with sterile water wash remained on surface soup, cordyceps militaris sporocarp is inserted on tripod, build bell jar again.With gauze that bell jar bottom periphery plug is good, and fall 0.1% mercuric chloride water on the gauze, at last a whole set of spore harvester is taken out from inoculating hood and put into insulating box (about 25 degrees centigrade), spore is fallen, treat to have on the medium Cordyceps Militaris to drop out now, the single or multiple bacterium colonies of picking move to be connected on the test tube slant medium and cultivate after (lucifuge is cultivated under 15 degrees centigrade of one 20 degrees centigrade of constant temperatures) cultivate successfully under aseptic condition.Can move and connect, expand system.
(3), the female enlarged culture of planting of test tube
Through make the female kind of test tube with separating method after, also need further tube to transplant enlarged culture.Female plantation is expanded numerous except increasing greatly the bacterial classification quantity, can also effectively avoid the aging or undergrowth of bacterial classification, prevents or eliminate microbial contamination.
The female enlarged culture based formulas of planting of test tube:
1, peptone 10 grams, glucose 10 grams contain fat silkworm chrysalis 10 grams, whole milk powder 10 gram potassium dihydrogen phosphates 15 grams, agar 2 grams, 1000 milliliters in water.
2, glucose 10 grams, peptone 10 grams, yeast extract 1 gram, potassium dihydrogen phosphate 1 gram, magnesium sulfate 0.5 gram, agar 20 grams, 5 milliliters in growth hormone, 1000 milliliters in water.
3, peeled potatoes 200 grams (liquor), glucose 20 grams (or sucrose 40 grams), potassium dihydrogen phosphate 1.5 grams, agar 20 grams, the vitaminB10 gram increases spirit 0.5 gram, 1000 milliliters in water.Sexual Chinese caterpillar fungus hypha growth is slower, under 20 degrees centigrade constant temperature, generally need cover with the inclined-plane through 3-4 month cultivate one's ability, when seeing the test tube slant medium 2 centimetres of big bacterium colonies of foretelling are arranged, bacterium projection and surface are tortuous uneven, and color is that orange colour illustrates promptly that test tube has been cultivated with mother and finishes.
(4), go out the grass test
The bacterial classification of the sexual separation of Chinese caterpillar fungus must be made the grass test before plantation, the new bacterial classification that separates of Chinese caterpillar fungus not necessarily can both go out grass, therefore must test through the strict grass that goes out, can put into production after the sexual reproduction proterties of mycelia is stable, for go out in the grass test performance position go out grass early, the higher bacterial classification of output will in time expand numerous and preservation.
(5), separate tissue is made female the kind
Selecting for use good, pure, healthy and strong, of the right age natural cs to make asexual hyphostroma and separate, is the key that realizes high yield, high-quality.The asexual cultivation algebraically of Cordyceps militaris is divided into 3 grades of female kind, original seed and cultivated speciess, the female kind is the mycelium that separate tissue is cultivated, original seed and cultivated species are to enlarge to cultivate to form bacterial classification on the female basis of planting of test tube, the female kind should not directly be cultivated usually, tube enlarges and the making original seed and be mainly used in, and as the bacterial classification preservation, original seed then can be used for cultivation and production, also can continue expanding propagation and become cultivated species to be mainly used in cultivation and production.
Two, the female kind changeed original seed and cultivated species, comprises the following steps:
(1), culture medium preparation
1, sucrose 10 grams, peptone 10 grams, agar 15 grams, magnesium sulfate 0.5 grams, 1000 milliliters of vitamin B5 sheets.
2, glucose 20 grams, agar 20 grams, potato 200 grams (liquor), Cobastab .3 sheet, peptone 5 grams, potassium dihydrogen phosphate (reagent) 0.5 gram.
Compound method: now various fuels are taken by weighing equivalent, close and mix well.Agar is melted the heating of 1000 ml waters again, then fuel is poured into, boiling time is 5-10 minute, and regulated value is PH5-6 thereafter, and the packing test tube is standby through putting into the inclined-plane behind the autoclaving while hot.
(2) female tube of planting enlarges
Inoculating hood and all apparatus.Will carry out strict sterilization in advance, the inoculation personnel must wash one's hands in case assorted cingula is gone into the Lysol solution of soap or 2% before inoculation.
The tube operation, mother's kind is planted experimentally a mouthful calcination on alcolhol burner flame, extract tampon simultaneously, then with all instruments also in the alcolhol burner calcination, wait and carry out mother after the cooling and plant and cut tube, instrument is not run into tube wall during tube, in the other test tube central authorities that change over to rapidly of flame, note medium not being scratched, bacterial classification is adhered on the test tube wall, extract inoculating tool out, tampon is screwed.
One female plants test tube and once can expand and connect 25 one 35 test tubes, and inoculation finishes the test tube that connects is labelled, indicates the bacterium name date, the inoculation people, 7-10 props up to a bundle and tightens test tube top with brown paper, puts into incubator after the end and cultivates, for fear of temperature jump, influencing mycelial growth grows, be preferably in the insulating box and cultivate, generally under 20 degrees centigrade～28 degrees centigrade, heat insulating culture is about 10 days, secondary is female plants (can cultivate cultivated species, also can directly plant).
The method of using is moved and is connect the female kind of the Cordyceps militaris secondary that expands system, also can directly be used for producing and cultivate, directly plantation.In general 1 former bacterial classification expands numerous number of times and should not then easily cause spawn degeneration too much.Promptly can make pure culture and preserve, generally must not be above 3 times.
Three, the preparation of suspension bacterial classification comprises the following steps:
(1), medium preparation
1, potassium dihydrogen phosphate 3 grams, salt 3 grams, vitamin B5 sheet, glucose sugar 15 grams, magnesium sulfate 0.5 gram, monosodium glutamate 0.2 gram, analysis for soybean powder 15 grams, 1000 milliliters in water.
2, corn flour 12 grams, dusty yeast 3 grams, potassium dihydrogen phosphate 1 gram, glucose 22 grams, analysis for soybean powder 10 grams, Cobastab, 180-200 milli, magnesium sulfate 0.5 gram, 1000 milliliters in water.
3, glucose sugar 35 grams, peptone 25 grams, potassium dihydrogen phosphate 1 gram, salt 2.5 grams, vitamin B3 sheet.
Compound method: take by weighing by above-mentioned equivalent and to put into pot and boiled 10-15 minute, pour in the measuring cup precipitation then into after 15-20 minute, upper strata liquid is packed in the 500 mL of saline bottles, every bottled amount is the 200-250 milliliter.
(2) sterilization
Use portable high-pressure sterilizing pot, will tighten spiral behind the bottled pot of suspension, the intensification firepower should strengthen gradually, and air pressure reaches 49 kPas of (0.5 kg/cm.) time opens air bleeding valve and discharge cold air.When treating that manometer returns zero, shut air bleeding valve again and heat up again, when temperature is raised to 147 (1.5 kg/cm.) time when keeping cutoff in 40 1 60 minutes cooling to treat that manometer returns zero, open air bleeding valve, discharge entrained steam and take the lid of the pot off, it is stand-by to take out sterilized solution cooling back.
(3) inoculated and cultured
Overall process is all carried out under aseptic condition, and relevant operation measure sees also solid spawn, and every secondary test tube bacterium can connect 4-6 bottle suspension.The suspension that connects is placed on dark place, under 20-28, cultivated 5 days, rock bottle when seeing bottle inner suspension liquid surface adularescent mycelia, make bottle interior mycelia and liquid even, suspension all is under natural temperature mostly, cultivate, be not in the insulating box kind, great majority white hypha occurs and rock not open, and this is a normal phenomenon, the conidium of the Cordyceps Militaris of surface caking has but entered suspension just as pollen is invisible to the naked eye when rocking, and can cultivate use again after 4-5 days after this kind phenomenon occurs.
Four, liquid spawn is made, and comprises the following steps:
(1), liquid spawn culture medium prescription
1, peptone 12 gram, dusty yeast 25 grams, corn flour 10 grams, analysis for soybean powder 10 grams, potassium dihydrogen phosphate 0.5 gram, magnesium sulfate 0.3 gram, monosodium glutamate 0.1 gram, glucose 20 grams, salt 0.3 gram, Cobastab: 1000 milliliters of 100 milligrams, water.
2, glucose 10 gram, potassium dihydrogen phosphate 0.1 gram, magnesium sulfate 1 gram, calcium chloride 1 gram, amino acid composite powder 5 grams, peeled potatoes 250 grams (liquor), cicada pupa 20 grams, water are 1000 milliliters.
3, potassium dihydrogen phosphate 0.05 gram, Cobastab, 1000 milliliters of 1150 milligrams, peptone 15 grams, glucose 25 grams, dusty yeast 10 grams, magnesium sulfate 0.05 gram, salt 8 grams, water.The bottling sterilization.
Select above-mentioned prescription for use, weigh up various raw materials by requirement (this sentences 1000 milliliters of composts or fertilisers of cultivating is example), put into pot, adding 1 300 milliliters of heating of water boiled 5-10 minute, cooling, precipitate 1 hour, get the upper strata stillness of night and pack in the wide-mouth bottle, loading amount is 7/10 of a bottle, it is defoamer that adding 3-6 drips edible oil, culture fluid produces the bubble foam when preventing to ventilate, and bottleneck with replacing the tampon plug good, is placed in the pot, carry out autoclaving, high pressure was sterilized 40-60 minute down for 1 47 kPas, and sterilization knot card naturally cools to below 30 ℃, prepares inoculation.
(2), inoculated and cultured
By nothing bundle operation requirement, access secondary kind and cultivated species get final product in inoculating hood, and the concrete operations main points are made identical with female kind.Be placed under 20 ℃ one 25 ℃ the condition static cultivation after the inoculation 2 days, observing the liquid primary surface does not have heterochromatic, clear can the ventilation, start little throughput earlier, oxygen blast 3 days, restarted the atm number oxygen blast 3-4 days, logical like this oxygen stirs better, in the incubation, at least check once every day, observe the liquid base and whether become turbid, as the explanation of becoming turbid is infected, should destroy, if clear in the liquid base have, the mycelia sheet appears as normally, treats that mycelia sheet diameter reaches 0.3-1 millimeter goods mycelium pellet and is full of the liquid base, illustrates that liquid spawn completes.
Five, the Cordyceps militaris artificial cultivation comprises the following steps:
(1), presses culture medium prescription and make medium
1, fries rice 93%, glucose 2%, peptone 2%, citric acid 0.3%, magnesium sulfate 0.2%, dried silkworm chrysalis meal 2.5%, vitaminize B in addition, 0.01%.
2, sorghum rice 85%, dried silkworm chrysalis meal 10%, sucrose 2%, peptone 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, dusty yeast 0.8%, vitaminize B in addition, 0.01%.
3, millet 95%, glucose 3.5%, yeast extract 1.2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.2%, vitaminize B in addition, 0.01%.
4, sorghum rice 45%, maize pulp 40%, millet 10%, sucrose 2%, peptone 2%, dusty yeast 0.8%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%.
5, rice 50%, wheat bran 25%, and corn flour 10%, glucose (or sucrose) 2%, wood chip (broadleaf tree is advisable) 10%, (the stiff silkworm in available shop of Chinese medicines replaces 12% to dried silkworm chrysalis meal, urea 0.1%, magnesium sulfate 0.9%, vitaminize B in addition, 0.01%.
(2), spice bottling
Above-mentioned medium is chosen any one kind of them, and it is even to take by weighing the equivalent spice, and water content must remain on 60% one 65%, pH value 5-7, be respectively charged in 500 milliliters of Cans, can adorn about siccative 50 grams for every bottle, fuel moisture is meter in addition, available thereafter PEF or polypropylene film seal.
(3), sterilization
Sterilization has two kinds of methods: a kind of is autoclaving; A kind of is normal-pressure sterilization.Here only talk normal-pressure sterilization, normal-pressure sterilization helps big row and produces, capacity is big, sterilization time must remain on and get final product in 8-10 hour, and beginning low temperature carries out 3-4 hour (as quick heating, the too high meeting of temperature causes that bottle is broken cruelly) under 50 ℃, thereafter carrying out 100 ℃ of high temperature burnt greatly to 4-6 hour, the cooling that takes the dish out of the pot after having sterilized, inoculation is prepared in the cooling back, and inoculation temperature must not be above 20 ℃.
(4), inoculation
Under aseptic condition, operate, it is prosperous burning at first to possess a coal briquette stove, extracts liquid spawn with sterile syringe above flame, injects the blake bottle kind that bacterium is crossed in death of monks or nuns equably with every bottle of 5-10 milliliter, inoculation finishes, and changes blake bottle over to the cultivation indoor cultivation.
(5), culturing room cultivates
Before blake bottle enters the chamber, must carry out disinfection in advance earlier, after the sterilization, can be placed on the bedstead taking over the bottle of planting, each bottle stays point space slightly, be beneficial to ventilation and heat, the simultaneously indoor necessary preference temperature that keeps, 15-25 days mycelia can be covered with medium generally speaking, and form fruit body primordium, 45 days one 60 days can be ripe, and Chinese caterpillar fungus is cultivated management can be divided into two stages.
1, temperature: the north winter, careless mycelia all can grow at 6 ℃ one 30 ℃, stopped growing growth retardation more than 30 ℃ below 6 ℃, very be dead, preference temperature is 5 ℃ one 25 ℃, the hyphal development initial stage, room temperature is 15 ℃ one 20 ℃, and the later stage is 23 ℃ one 25 ℃, surpasses 25 ℃ and is difficult for forming stroma.
2 light: in the mycelia stage, the dark hyphal development of pipeline is very fast, but light intensity then is difficult for bearing stroma, and poor growth, enters generative growth phase too early, influences output and quality, must wait mycelia to cover with and could see light at the bottom of a bottle face is pricked bottle.
3, humidity: Chinese caterpillar fungus seals growth in bottle, the air humidity influence is little, but it is long-time dry, also can make water in bottle divide slowly evaporation, influence later stage mycelial growth and fruit body and form, for this reason, will be suitably at flooring water spray humidification, early stage, humidity remained on about 65%, crossed the wet assorted bacterium that easily gives birth to, and was difficult for forming stroma.
4, oxygen: scarlet caterpiller fungus mycelium stage respiratory capacity is less, indoor need not ventilated, need not the specific condition management, can cover with medium 15-25 days mycelia, the growth of Cordyceps militaris will also soon enter generative growth phase by nourishing and growing, the position that this stage puts bottle can not be touched, because dynamic temperature degree variable effect growth.
5, growing way: after mycelia is covered with bottle, and the bulge projection appears, and begin to see that the light annesl goes out careless colour-change period, ventilate at 6-8 o'clock on the drought every day and see light, after treating that mycelia all transfers yellow to by white, can suitably increase scattered light every day, the annesl initial stage, temperature with about 21 ℃ for well, after annesl is finished, temperature remains on about 23 ℃, and humidity remains on more than 85%, and illumination every day colour-change period must not be less than 10 hours.See that Huang promptly ends, light can not shine continuously, and colour-change period needs 2-8 days, promptly begins to form on charge level millet shape original hase (being button) behind the annesl.
(6), final-period management
1, when most of charge level forms original hase, temperature can remain on about 23 ℃, humidity is 85-90%, humidity too Da Yi is brought out medium to produce too much aerial hyphae unfavorable to sporophore growth, the too little medium that makes easily of humidity loses moisture content too early and influences output, for keeping suitable humidity, sprayable water is in ground, 3-4 days every day.
2, sporophore growth stage management
(1) temperature: the optimum temperature that forms cordyceps militaris sporocarp is 20 ℃ one 25 ℃, and therefore, this stage is the energy temperature control to the greatest extent, is beneficial to fruit body and forms and growth, and temperature control is below 25 ℃, and surpassing 28 ℃ can not the strong point stroma.
(2) light: after mycelia is covered with, can increase natural lighting, mycelia gradually becomes orange colour or orange colour by white after seeing light, grain of rice shape orange colour button promptly can appear in media surface behind the mycelia secretion pigment, be fruit body after the button elongation, can not continuous illumination after turning round, continuous illumination can not generate fruit body.
(3) air humidity: the sporophore growth stage will be increased to 85% one 90% to indoor air humidity, distribute to reduce the water in bottle branch, cordyceps militaris sporocarp forms and will lean on moisture content to absorb nutrition, cell is expanded, total cellular score up to ripe Chinese caterpillar fungus after original hase forms does not increase, and the increase of its whole fruit body is each cell absorption moisture content and nutrition and the result that increases, for this reason, moisture content must be in fuel, filled up, just the Chinese caterpillar fungus high yield can be made.
(4) oxygen: even Chinese caterpillar fungus still can normal growth in the bottle of sealing.That is to say that it is less to the demand of oxygen, for this reason, in whole growth process, reduce water in bottle part as far as possible and distribute, need not to remove sealing film, when centimetre high left and right sides of fruit body length to 2 in the bottle, on sealing film, prick several holes with bodkin and get final product.
Again through about 15 days, the Chinese caterpillar fungus stroma can be ripe by above-mentioned condition, and about up to 8 centimetres, the fruit body surface has the yellow powder thing to occur and can gather.
(7), gather
Long to the 5-8 centimeter length, promptly ripe when the Chinese caterpillar fungus stroma, remove sealing film, Jiang's fruit body is extracted, dry or dry all can, preserve as need, moisture content is lower than 3% to prevent moldy metamorphism, seals with bag film after doing.
Claims (6)
1. production of Cordyceps militaris and deep working technology, feature are that the technical scheme that adopts is: production of Cordyceps militaris and deep working technology comprise the following steps: that (one) female kind prepares; (2) the female kind changeed original seed and cultivated species: the preparation of (three) suspension bacterial classification, the preparation of (four) liquid spawn; (5) artificial cultivation of Cordyceps militaris spawn.
2. production of Cordyceps militaris according to claim 1 and deep working technology, feature is that female the kind prepares the spore separation method that adopts, under aseptic condition, utilize the mushroom fruit body to launch the principle of spore voluntarily, spore is scattered on the synthetic medium, by cultivating, obtain original fungus strain body, and then move the access slant tube and cultivate; Described female kind prepares and comprises that (1) makes medium: the female kind of (2) test tube prepares: the enlarged culture of the female kind of (3) test tube; (4) go out the grass test; (5) separate tissue is made female the kind.
3. production of Cordyceps militaris according to claim 1 and deep working technology, feature is that original seed and cultivated species derive from female expansion of planting, original seed is the natural cs bacterial strain, after artificial training, propose the high-quality bacterium colony and change solid culture medium over to, obtain the female kind of one-level through different temperature, humidity, nutrients of each stage; Female kind can only be made bacterial classification and use, must not directly cultivate, so must make the original seed tube cultivates, general original seed also is difficult for directly cultivation, because the original seed source is few, the cost height needs repeatedly tube to cultivate into cultivated species, and female kind changes original seed and cultivated species comprises: (1) culture medium preparation, (2) female tube of planting enlarge.
4. production of Cordyceps militaris according to claim 1 and deep working technology, feature is that described suspension strain preparation comprises: the preparation of (1) medium, (2) sterilization, (3) inoculated and cultured.
5. production of Cordyceps militaris according to claim 1 and deep working technology, feature is that liquid spawn is made as: adopt the single-stage cultivation to be liquid production of hybrid seeds express method again, progressively realize multistage cultural method, realize that the stable high yield cycle short, FFI high-efficiency strain, single-stage is cultivated with short production cycle, can turn out first Chinese caterpillar fungus liquid spawn in 8-10 days, output is bigger, can produce 1000-2000 milliliters of liquid bacterial classification as required for 1 time, and can produce continuously, cultivation is convenient, as long as liquid spawn is sprayed onto media surface, and can to send out bacterium fast, be solid send out the bacterium time half, the output height, be 2 times of solid bacterium output, and fundamentally solved infection time, the success rate height has been stopped assorted bacterium fully with special filter, saccharomycetic infection, success rate is up to more than 95%, need 6-8 month time with conventional method plantation Chinese caterpillar fungus, adopt liquid spawn, only need 50-60 days, shortened the Chinese caterpillar fungus production cycle greatly, described liquid spawn is made and comprises (1) liquid spawn culture medium prescription, (2) inoculated and cultured.
6. production of Cordyceps militaris according to claim 1 and deep working technology, feature is that described Cordyceps militaris spawn artificial cultivation comprises: (1) is pressed culture medium prescription and is made medium, the bottling of (2) spice; (3) sterilization; (4) inoculation; (5) culturing room cultivates: (6) final-period management; (7) gather.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511313A (en) * | 2011-12-29 | 2012-06-27 | 重庆沙科农业开发有限公司 | Cordyceps militaris cultivating greenhouse and Cordyceps militaris cultivating method |
| CN103070009A (en) * | 2012-12-18 | 2013-05-01 | 徐州鸿宇农业科技有限公司 | Method for cultivating silkworm larvae into silkworm cordyceps by simulating natural ecological environment |
| CN103125275A (en) * | 2013-03-15 | 2013-06-05 | 熊艳 | Cultivation method of cordyceps militaris |
| CN103563655A (en) * | 2013-10-17 | 2014-02-12 | 烟台金刚生源生物科技有限公司 | Technique for cultivating living pupation cordyceps |
| CN103688748A (en) * | 2013-12-13 | 2014-04-02 | 福建农林大学 | Epimedium cordyceps militaris cultivation and product processing method |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN104472221A (en) * | 2014-12-22 | 2015-04-01 | 四川藏宝虫草生物科技有限公司 | Method for fermenting mycelia from natural ophiocordyceps sinensis fruiting bodies |
| CN104996166A (en) * | 2015-07-01 | 2015-10-28 | 天津齐心菌类种植有限公司 | Cultivation method for liquid strains |
| CN105230336A (en) * | 2015-08-31 | 2016-01-13 | 佛山市高明区鹏鹄农业发展有限公司 | Method for planting cordyceps flower by microbe |
| CN107173051A (en) * | 2017-05-20 | 2017-09-19 | 天津市东方中滨农业科技有限公司 | A kind of method and its application of many spore crossbreeding of Cordyceps militaris |
| CN111919665A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cordyceps militaris cultivation inoculation method |
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2011
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511313A (en) * | 2011-12-29 | 2012-06-27 | 重庆沙科农业开发有限公司 | Cordyceps militaris cultivating greenhouse and Cordyceps militaris cultivating method |
| CN103070009A (en) * | 2012-12-18 | 2013-05-01 | 徐州鸿宇农业科技有限公司 | Method for cultivating silkworm larvae into silkworm cordyceps by simulating natural ecological environment |
| CN103125275B (en) * | 2013-03-15 | 2015-06-10 | 熊艳 | Cultivation method of cordyceps militaris |
| CN103125275A (en) * | 2013-03-15 | 2013-06-05 | 熊艳 | Cultivation method of cordyceps militaris |
| CN103563655A (en) * | 2013-10-17 | 2014-02-12 | 烟台金刚生源生物科技有限公司 | Technique for cultivating living pupation cordyceps |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN103688748A (en) * | 2013-12-13 | 2014-04-02 | 福建农林大学 | Epimedium cordyceps militaris cultivation and product processing method |
| CN103688748B (en) * | 2013-12-13 | 2015-06-03 | 福建农林大学 | Epimedium cordyceps militaris cultivation and product processing method |
| CN104472221A (en) * | 2014-12-22 | 2015-04-01 | 四川藏宝虫草生物科技有限公司 | Method for fermenting mycelia from natural ophiocordyceps sinensis fruiting bodies |
| CN104996166A (en) * | 2015-07-01 | 2015-10-28 | 天津齐心菌类种植有限公司 | Cultivation method for liquid strains |
| CN105230336A (en) * | 2015-08-31 | 2016-01-13 | 佛山市高明区鹏鹄农业发展有限公司 | Method for planting cordyceps flower by microbe |
| CN107173051A (en) * | 2017-05-20 | 2017-09-19 | 天津市东方中滨农业科技有限公司 | A kind of method and its application of many spore crossbreeding of Cordyceps militaris |
| CN111919665A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cordyceps militaris cultivation inoculation method |
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