CN102250198A - Method for extracting phytosterol from stems of dragon fruits - Google Patents
Method for extracting phytosterol from stems of dragon fruits Download PDFInfo
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- CN102250198A CN102250198A CN2011101288050A CN201110128805A CN102250198A CN 102250198 A CN102250198 A CN 102250198A CN 2011101288050 A CN2011101288050 A CN 2011101288050A CN 201110128805 A CN201110128805 A CN 201110128805A CN 102250198 A CN102250198 A CN 102250198A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention discloses a method for extracting phytosterol from stems of dragon fruits (Latin name: Hylocereus undatus), which is characterized by comprising the following steps of: adding dry dragon fruit stem powder passing a 20-mesh sieve into an extraction kettle, heating to the temperature of between 45 and 55DEG C, introducing mixed fluid of an entrainer and CO2 gas, extracting under the pressure of 25-35Mpa at the flow rate of the mixed fluid of 15-30L/h for 3 to 5 hours, spraying the fluid in the extraction kettle into a cyclone separator with the pressure of 4-10Mpa and the temperature of 25-40DEG C, separating out the solid phytosterol from a fluid phase, and collecting the phytosterol at the bottom of the cyclone separator. The raw material is extracted by a CO2 supercritical fluid extraction technology, the safety and environment friendliness are enhanced, and the extraction efficiency and product purity are improved.
Description
Technical field
The present invention relates to a kind of method of from flue stem end stalk, extracting natural phytosterin.
Background technology
Plant sterol has reducing cholesterol, reducing blood-fat, effect such as anti-oxidant has caused people's great attention, and plant sterol is described as " key of life " world's heart tissue, American Heart Association, BHF, German nutrition medical science and world authoritative institutions such as dietotherapy meeting, Australian heart association and is all recommended energetically by scientists; 47 authorized by states in the whole world also are applied to field of food.Hallikianen (2008) etc. give that the adult of 22 hyperlipidemia takes 0.8,1.6,2.4 respectively, 4 weeks of plant sterol of 3.2g/d, found that the total cholesterol concentration fall of respectively organizing serum is divided into 2.8%, 6.8%, 10.3% and 11.3%, LDL concentration has then descended 1.7%, 5.6%, 9.7% and 10.4% respectively, except that the lowest dose level group, the fall of other group all has statistical significance.The double blind control of Sierksma (2008) etc. is discovered, compares with control group, takes soyasterol 0.8g/d, can significantly reduce TC and LDL in the blood after 3 weeks, but can not cause the decline of other liposoluble vitamin in the blood such as carotene level.Therefore, scientists generally believes that the plant sterol of absorption or additional capacity helps to reduce crowd's evidence of coronary heart diseases now.It has huge market outlook.At present, China's plant sterol mainly is the by product-deodorization distillate with grease production, by obtaining after the processing of methods such as chemicrystallization method, complexometry, molecular distillation method and column adsorption method.
Summary of the invention
Purpose of the present invention is exactly the method by the CO2 supercritical liquid extraction technique, extracts plant sterol from the flue stem end.
The technical solution used in the present invention is:
A kind of method of extracting plant sterol from the flue stem end, described method is: got 20 mesh sieve exsiccant flue stem end powder and add in the extraction kettle, and be warming up to 45～55 ℃, and fed and guide and support agent and CO
2The mixed mixed stream of gas, control pressure is 25～35Mpa, the flow of mixed stream is 15～30L/h, extract after 3～5 hours, fluid in the extraction kettle is sprayed into 4～10Mpa (in preferred 5～7Mpa), 25～40 ℃ Cyclonic separator, solid-state plant sterol separates mutually from fluid separates out, and Cyclonic separator bottom is collected and promptly obtained plant sterol;
Hylocereus undatus has another name called Green Dragon fruit, red dragon fruit, and Latin is called Hylocereus undatus, is Cactaceae triangular prism platymiscium, originates in Central America torrid zone desert area, belongs to the typical tropical plant.Hylocereus undatus is nutritious, function is unique, and it contains rare vegetalitas albumin of general plant and anthocyanidin, abundant VITAMIN and water-soluble meals fiber.Hylocereus undatus is to introduce Taiwan by the Nan Yang, is introduced ground cultivations such as the southern Guangxi in Hainan Province and continent, Guangdong again by the Taiwan improvement.Hylocereus undatus is gained the name like the outer unicorn of flood dragon because of its appearance meat scale.The present invention adopts the dry stem of Hylocereus undatus plant to be used as medicine.
Described guide and support agent be water, ethanol, acetone with volume ratio 1: 4～7: 2～5 blended solution, 5%～20% of the quality that described quality consumption of guiding and supporting agent is a flue stem end powder.
Described mistake 20 mesh sieve exsiccant flue stem end powder are meant its moisture content≤5%, usually raw material flue stem end is carried out dry pre-treatment, make its moisture content≤5%, pulverize the back by pulverizer then and cross 20 mesh sieves, promptly got 20 mesh sieve exsiccant flue stem end powder.
Describedly guide and support agent and normally pump in the pipeline, with the ducted CO in another road
2Be mixed stream after the mixing, main component is CO in the mixed stream
2Usually to be the Flow-rate adjustment of will guide and support agent guide and support agent and be dispersed in CO as far as possible uniformly for guiding and supporting total consumption/reaction times of agent, making preferred way
2In, also can add CO with big flow at the very start but guide and support agent in reaction
2In, effect of extracting is had no significant effect.Owing to guide and support the agent consumption with respect to CO
2Seldom, the flow of mixed stream mainly is exactly CO
2Flow.
Fluid in the described Cyclonic separator is the gas-liquid mixed attitude mutually, be positioned at Cyclonic separator middle and upper part, and by being positioned at the pipeline derivation on Cyclonic separator top, and solid-state plant sterol separates mutually from fluid and separates out, fall within Cyclonic separator bottom, collect from Cyclonic separator bottom and can obtain plant sterol.CO in the described Cyclonic separator in the fluid
2Gas delivery is come out, and can pump into again by air pump to carry out recycle in the extraction kettle.
Beneficial effect of the present invention is:
1. utilize CO
2Supercritical liquid extraction technique advances extraction to raw material, and safer, environmental protection has also improved extraction efficiency and product purity.
2. Hylocereus undatus is a kind of economic fruit, and the present invention utilizes the by product flue stem end in its production, and raw material sources have guaranteed its green as the additive source, pollution-free in natural phant.
Embodiment
The present invention will be further described with specific embodiment below, but protection scope of the present invention is not limited thereto.
Embodiment 1:
Exsiccant flue stem end (moisture 3.5%) crosses 20 mesh sieves after crushed, gets 100 gram powder transfer to extraction kettle.Temperature to 45 ℃ in the rising extraction kettle pumps into CO simultaneously
2With guide and support the agent mixed stream, the volume ratio of guiding and supporting in the agent to water, ethanol, acetone is 1: 7: 2 blended solution, total consumption of guiding and supporting agent is 20g, will guide and support agent to pump in the pipeline, with the ducted CO in another road
2Be mixed stream after the mixing, make the extraction kettle internal pressure reach 25Mpa, the flow of control mixed stream is 15L/h, to raw material abstraction 3 hours, makes fluid spray into the Cyclonic separator after extraction is finished.Cyclonic separator internal gas pressure is that 5Mpa, temperature are 40 ℃, and solid-state plant sterol separates mutually from fluid separates out, and collects and promptly gets natural phytosterin 2.85g.Utilize high performance liquid chromatography to detect, reclaim sterol purity 87.1% reclaiming sterol.
Embodiment 2:
Exsiccant flue stem end (moisture 4%) crosses 20 mesh sieves after crushed, gets 100 gram powder transfer to extraction kettle.Temperature to 50 ℃ in the rising extraction kettle pumps into CO simultaneously
2With guide and support the agent mixed stream, guiding and supporting agent and be water, ethanol, acetone is 1: 4: 5 blended solution with volume ratio, total consumption of guiding and supporting agent is 5g, make the extraction kettle internal pressure reach 35Mpa, and with the flow of 30L/h to raw materials treated extraction 5 hours, make fluid spray into the Cyclonic separator after extraction is finished.Cyclonic separator internal gas pressure is that 5Mpa, temperature are 25 ℃, and solid-state plant sterol separates mutually from fluid separates out, and collects and promptly gets natural phytosterin 3.37g.Utilize high performance liquid chromatography to detect, must reclaim sterol purity is 84.6%.
Embodiment 3:
Exsiccant flue stem end (moisture 5%) behind mistake 20 mesh sieves, is got 100 gram powder transfer to extraction kettle after crushed.Temperature to 55 ℃ in the rising extraction kettle pumps into CO simultaneously
2With guide and support the agent mixed stream, guiding and supporting agent and be water, ethanol, acetone is 1: 5: 4 blended solution with volume ratio, total consumption of guiding and supporting agent is 7g, make the extraction kettle internal pressure reach 30Mpa, and with the flow of 20L/h to raw materials treated extraction 4 hours, make fluid spray into the Cyclonic separator after extraction is finished.Cyclonic separator internal gas pressure is that 7Mpa, temperature are 30 ℃, and solid-state plant sterol separates mutually from fluid separates out, and collects and promptly gets natural phytosterin 3.23g.Utilize high performance liquid chromatography to detect, reclaiming sterol purity is 90.1%.
Claims (3)
1. a method of extracting plant sterol from the flue stem end is characterized in that described method is: got 20 mesh sieve exsiccant flue stem end powder and add in the extraction kettle, and be warming up to 45～55 ℃, and fed and guide and support agent and CO
2The mixed mixed stream of gas, control pressure is 25～35Mpa, the flow of mixed stream is 15～30L/h, extract after 3～5 hours, fluid in the extraction kettle is sprayed in 4～10Mpa, 25～40 ℃ the Cyclonic separator, solid-state plant sterol separates mutually from fluid separates out, and described Cyclonic separator bottom is collected and obtained plant sterol;
Described guide and support agent be water, ethanol, acetone with volume ratio 1: 4～7: 2～5 blended solution, 5%～20% of the quality that described quality consumption of guiding and supporting agent is a flue stem end powder.
2. the method for claim 1 is characterized in that described mistake 20 mesh sieve exsiccant flue stem end powder are meant its moisture content≤5%.
3. the method for claim 1 is characterized in that the CO in the fluid in the described Cyclonic separator
2Gas delivery is come out, and pumps into again by air pump and carries out recycle in the extraction kettle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011101288050A CN102250198B (en) | 2011-05-18 | 2011-05-18 | Method for extracting phytosterol from stems of dragon fruits |
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| CN2011101288050A CN102250198B (en) | 2011-05-18 | 2011-05-18 | Method for extracting phytosterol from stems of dragon fruits |
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| CN102250198A true CN102250198A (en) | 2011-11-23 |
| CN102250198B CN102250198B (en) | 2013-02-27 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402957A (en) * | 2014-11-21 | 2015-03-11 | 河南大学 | Method for extracting purified phytosterol from corn stigma |
| CN105661513A (en) * | 2016-01-21 | 2016-06-15 | 安徽农业大学 | Pitaya stem powder difficult in moisture absorption and production method thereof |
| CN105685869A (en) * | 2016-01-21 | 2016-06-22 | 安徽农业大学 | Dragon fruit stem chewable tablets and preparation method thereof |
| CN107383138A (en) * | 2017-06-27 | 2017-11-24 | 广西南宁桂知科技有限公司 | A kind of method that natural phytosterin is extracted from dragon fruit bough |
| CN107691483A (en) * | 2017-08-31 | 2018-02-16 | 寿光园林建设集团有限公司 | A kind of complex microorganism soil insecticide and preparation method thereof |
| CN110157575A (en) * | 2019-06-10 | 2019-08-23 | 广西桂林市鑫珍生物科技有限责任公司 | A kind of preparation method of dragon fruit sterol vegetarian feast |
| CN113603803A (en) * | 2021-08-27 | 2021-11-05 | 广西壮族自治区农业科学院 | Method for simultaneously extracting phytosterol and polysaccharide from dragon fruit stems |
Citations (2)
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| CN1263103A (en) * | 1999-11-19 | 2000-08-16 | 上海交通大学 | Supercritical fluid extraction method of high-purity sterol |
| CN1631480A (en) * | 2004-11-19 | 2005-06-29 | 中国科学院山西煤炭化学研究所 | Method by extracting samara essence by supercritical CO2 |
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2011
- 2011-05-18 CN CN2011101288050A patent/CN102250198B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1263103A (en) * | 1999-11-19 | 2000-08-16 | 上海交通大学 | Supercritical fluid extraction method of high-purity sterol |
| CN1631480A (en) * | 2004-11-19 | 2005-06-29 | 中国科学院山西煤炭化学研究所 | Method by extracting samara essence by supercritical CO2 |
Non-Patent Citations (1)
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402957A (en) * | 2014-11-21 | 2015-03-11 | 河南大学 | Method for extracting purified phytosterol from corn stigma |
| CN104402957B (en) * | 2014-11-21 | 2016-04-06 | 河南大学 | A kind of method of extraction purification plant sterol from Stigma Maydis |
| CN105661513A (en) * | 2016-01-21 | 2016-06-15 | 安徽农业大学 | Pitaya stem powder difficult in moisture absorption and production method thereof |
| CN105685869A (en) * | 2016-01-21 | 2016-06-22 | 安徽农业大学 | Dragon fruit stem chewable tablets and preparation method thereof |
| CN105661513B (en) * | 2016-01-21 | 2018-07-17 | 安徽农业大学 | A kind of stems of dragon fruits powder not easy to moisture absorption and preparation method thereof |
| CN107383138A (en) * | 2017-06-27 | 2017-11-24 | 广西南宁桂知科技有限公司 | A kind of method that natural phytosterin is extracted from dragon fruit bough |
| CN107691483A (en) * | 2017-08-31 | 2018-02-16 | 寿光园林建设集团有限公司 | A kind of complex microorganism soil insecticide and preparation method thereof |
| CN110157575A (en) * | 2019-06-10 | 2019-08-23 | 广西桂林市鑫珍生物科技有限责任公司 | A kind of preparation method of dragon fruit sterol vegetarian feast |
| CN113603803A (en) * | 2021-08-27 | 2021-11-05 | 广西壮族自治区农业科学院 | Method for simultaneously extracting phytosterol and polysaccharide from dragon fruit stems |
| CN113603803B (en) * | 2021-08-27 | 2022-12-23 | 广西壮族自治区农业科学院 | Method for simultaneously extracting phytosterol and polysaccharide from dragon fruit stems |
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| CN102250198B (en) | 2013-02-27 |
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Effective date of registration: 20170525 Address after: Huang Lin Shan Industrial Zone Gan Yiting Town Road Yiwu City, Zhejiang province 322005 No. 20 Patentee after: Zhejiang Huatai Biological Technology Co., Ltd. Address before: 322005, No. E21, Xinke Road, Yiwu Economic Development Zone, Jinhua, Zhejiang Patentee before: Zhejiang Nongxing Biological Technology Co.,Ltd. |